Rh3AG118800

Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002737
Physical Location & Seq
Forward (+)
10211238 .. 10213950
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG118800.1

Sequence Viewer

Length: 900 bp
ATGGCGTCGGTGGGCCAGGCCGCGGAGGTGTACGAGCCGAAGCCACTCCAAGTCTGGCGAGCTCTGATGAACTGGTTGGGCTTCTTCTTCCAGATCATCGTTCAGATCCTCAGAGGCGCCCCCTGCCTCGCTCATTTCCTGTCGTACGTTCCTCTTCTCGCTTCTTCTTCTTCTTCTTTCAGGCCCTTGCCGGTCAGTGAAATCCCTCTCAACGACACGTCGTCCTCCGCTCACGCCAATCTCGAAAACGGCGTCGTAACCGACGATCACCGCCTTCAAAAGCTCACGGTAGTTCTTGACTTAGATGAGACGCTAGTGTGCGCGTACGAAACATCTAGCTTGCCTGCTATTGTTCGGACCCAAGCAACGGATGCTGGGTTGAAGTGGTTTGAGATAGAATGTGTATCTTCAGACAAGGAGTCTGATGGTAAACCGAAGATCAGTTATGTGACAGTGTTTGAACGTCCTGGTCTGCACGAGTTCTTGAAACGGATTTGTGAATTTGCTGATCTTGTCCTATTTACTGCTGGTCTTGAAGGATATGCTAGACCACTTGTTGACAGAATTGATGTGGAGAGCAGATTTAAGCTTCGTCTTTATCGGCCTTCTACAGTTACCACGGAAAACCGGGATCATGTGAAAGATCTGTCTTGTATATCGAAAGATCTCTGTCGAATTGTTATTGTTGACAACAACCCATTTAGTTTTCTGCTGCAACCGGTGAATGGAGTTCCATGTATTCCCTTTTCTGCTGGACATCCATATGATGATCAGCTCTTGGAGGTGCTGCTTCCACTCCTCCAGCACCTCGCCCAGCAAAAGGATGTGAGGCCTGTGCTTTATGAGAGATTCCACATGCCCGAATGGTTTCAAATGCATGGAGTTACTGTTTCTGGTTAG

Protein Analysis

299

Amino Acids

33.67

Weight (kDa)

5.48

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 95 - 277 4e-42 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 230
AccII CGCG 2 cut(s) 23, 323
AciI CCGC 4 cut(s) 21, 23, 228, 271
AclWI GGATC 2 cut(s) 100, 639
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 1 cut(s) 393
AcyI GRCGYC 3 cut(s) 5, 117, 252
AfaI GTAC 3 cut(s) 32, 146, 326
AfiI CCNNNNNNNGG 4 cut(s) 22, 367, 725, 820
AflIII ACRYGT 1 cut(s) 216
AgeI ACCGGT 1 cut(s) 718
AgsI TTSAA 6 cut(s) 278, 382, 461, 487, 536, 872
AhdI GACNNNNNGTC 2 cut(s) 220, 418
AjiI CACGTC 1 cut(s) 219
AjnI CCWGG 2 cut(s) 15, 466
AluBI AGCT 5 cut(s) 62, 283, 339, 589, 775
AluI AGCT 5 cut(s) 62, 283, 339, 589, 775
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 2 cut(s) 100, 639
AoxI GGCC 5 cut(s) 13, 18, 182, 602, 830
ApeKI GCWGC 2 cut(s) 712, 787
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AsiGI ACCGGT 1 cut(s) 718
Asp700I GAANNNNTTC 1 cut(s) 867
AspLEI GCGC 2 cut(s) 119, 323
AspS9I GGNCC 3 cut(s) 13, 183, 357
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 2 cut(s) 260, 733
AvaII GGWCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 64
BauI CACGAG 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 2 cut(s) 699, 774
BccI CCATC 1 cut(s) 419
BceAI ACGGC 1 cut(s) 265
BciT130I CCWGG 2 cut(s) 17, 468
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 3 cut(s) 314, 336, 546
BfmI CTRYAG 1 cut(s) 609
BfoI RGCGCY 1 cut(s) 120
BglII AGATCT 2 cut(s) 643, 664
BisI GCNGC 3 cut(s) 21, 713, 788
BlsI GCNGC 3 cut(s) 22, 714, 789
Bme1390I CCNGG 3 cut(s) 17, 468, 629
Bme18I GGWCC 1 cut(s) 357
BmeRI GACNNNNNGTC 2 cut(s) 220, 418
BmgBI CACGTC 1 cut(s) 219
BmgT120I GGNCC 3 cut(s) 13, 183, 357
BmiI GGNNCC 2 cut(s) 118, 359
BmrFI CCNGG 3 cut(s) 17, 468, 629
BmsI GCATC 1 cut(s) 361
BpmI CTGGAG 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 629
BsaHI GRCGYC 3 cut(s) 5, 117, 252
BsaJI CCNNGG 2 cut(s) 21, 618
BsaWI WCCGGW 1 cut(s) 718
BsaXI ACNNNNNCTCC 1 cut(s) 873
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 367, 725, 820
Bse118I RCCGGY 2 cut(s) 190, 718
Bse1I ACTGG 1 cut(s) 77
BseBI CCWGG 2 cut(s) 17, 468
BseDI CCNNGG 2 cut(s) 21, 618
BseGI GGATG 3 cut(s) 376, 757, 829
BseLI CCNNNNNNNGG 4 cut(s) 22, 367, 725, 820
BseMII CTCAG 1 cut(s) 124
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 1 cut(s) 788
BseXI GCAGC 2 cut(s) 699, 774
BseYI CCCAGC 2 cut(s) 374, 813
BsgI GTGCAG 1 cut(s) 458
Bsh1236I CGCG 2 cut(s) 23, 323
BshFI GGCC 5 cut(s) 15, 20, 184, 604, 832
BshNI GGYRCC 1 cut(s) 116
BshTI ACCGGT 1 cut(s) 718
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 3 cut(s) 191, 628, 719
BsiWI CGTACG 2 cut(s) 144, 324
BslI CCNNNNNNNGG 4 cut(s) 22, 367, 725, 820
BsmAI GTCTC 1 cut(s) 302
BsmBI CGTCTC 1 cut(s) 302
BsnI GGCC 5 cut(s) 15, 20, 184, 604, 832
Bsp1286I GDGCHC 1 cut(s) 64
Bsp143I GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
BspACI CCGC 4 cut(s) 21, 23, 228, 271
BspANI GGCC 5 cut(s) 15, 20, 184, 604, 832
BspCNI CTCAG 1 cut(s) 123
BspFNI CGCG 2 cut(s) 23, 323
BspLI GGNNCC 2 cut(s) 118, 359
BspPI GGATC 2 cut(s) 100, 639
BspT107I GGYRCC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 230
BsrFI RCCGGY 2 cut(s) 190, 718
BsrI ACTGG 1 cut(s) 77
BssAI RCCGGY 2 cut(s) 190, 718
BssECI CCNNGG 2 cut(s) 21, 618
BssMI GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
BssNI GRCGYC 3 cut(s) 5, 117, 252
BssSI CACGAG 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 476
Bst2UI CCWGG 2 cut(s) 17, 468
Bst4CI ACNGT 4 cut(s) 289, 454, 613, 889
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 3 cut(s) 5, 117, 252
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 3 cut(s) 60, 341, 345
BstDEI CTNAG 2 cut(s) 110, 301
BstDSI CCRYGG 2 cut(s) 21, 618
BstF5I GGATG 3 cut(s) 376, 757, 829
BstFNI CGCG 2 cut(s) 23, 323
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 2 cut(s) 119, 323
BstKTI GATC 9 cut(s) 96, 108, 268, 441, 511, 634, 646, 667, 772
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
BstMWI GCNNNNNNNGC 2 cut(s) 123, 371
BstNI CCWGG 2 cut(s) 17, 468
BstNSI RCATGY 1 cut(s) 859
BstSCI CCNGG 3 cut(s) 15, 466, 627
BstSFI CTRYAG 1 cut(s) 609
BstUI CGCG 2 cut(s) 23, 323
BstV1I GCAGC 2 cut(s) 699, 774
BstX2I RGATCY 3 cut(s) 105, 643, 664
BstYI RGATCY 3 cut(s) 105, 643, 664
BsuRI GGCC 5 cut(s) 15, 20, 184, 604, 832
BtgI CCRYGG 2 cut(s) 21, 618
BtrI CACGTC 1 cut(s) 219
BtsCI GGATG 3 cut(s) 376, 757, 829
BtsIMutI CAGTG 2 cut(s) 202, 459
Cac8I GCNNGC 3 cut(s) 60, 341, 345
CfoI GCGC 2 cut(s) 119, 323
Cfr10I RCCGGY 2 cut(s) 190, 718
Cfr13I GGNCC 3 cut(s) 13, 183, 357
Cfr42I CCGCGG 1 cut(s) 24
CseI GACGC 2 cut(s) 241, 319
Csp6I GTAC 3 cut(s) 31, 145, 325
CspAI ACCGGT 1 cut(s) 718
CviAII CATG 4 cut(s) 635, 735, 856, 878
CviQI GTAC 3 cut(s) 31, 145, 325
DdeI CTNAG 2 cut(s) 110, 301
DinI GGCGCC 1 cut(s) 118
DpnI GATC 9 cut(s) 95, 107, 267, 440, 510, 633, 645, 666, 771
DpnII GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
DriI GACNNNNNGTC 2 cut(s) 220, 418
Eam1104I CTCTTC 1 cut(s) 159
Eam1105I GACNNNNNGTC 2 cut(s) 220, 418
EarI CTCTTC 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 62
Eco147I AGGCCT 1 cut(s) 832
Eco24I GRGCYC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 183
EcoRII CCWGG 2 cut(s) 15, 466
EcoT22I ATGCAT 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 64
EgeI GGCGCC 1 cut(s) 118
EheI GGCGCC 1 cut(s) 118
Esp3I CGTCTC 1 cut(s) 302
FaeI CATG 4 cut(s) 638, 738, 859, 881
FatI CATG 4 cut(s) 634, 734, 855, 877
FauNDI CATATG 1 cut(s) 763
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 3 cut(s) 21, 713, 788
FokI GGATG 3 cut(s) 383, 744, 836
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 3 cut(s) 21, 713, 788
FspBI CTAG 3 cut(s) 314, 336, 546
GlaI GCGC 2 cut(s) 118, 322
GluI GCNGC 3 cut(s) 21, 713, 788
GsaI CCCAGC 2 cut(s) 378, 817
GsuI CTGGAG 1 cut(s) 785
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 5 cut(s) 15, 20, 184, 604, 832
HapII CCGG 3 cut(s) 191, 628, 719
HgaI GACGC 2 cut(s) 241, 319
HhaI GCGC 2 cut(s) 119, 323
Hin1I GRCGYC 3 cut(s) 5, 117, 252
Hin1II CATG 4 cut(s) 638, 738, 859, 881
Hin6I GCGC 2 cut(s) 117, 321
HinP1I GCGC 2 cut(s) 117, 321
HincII GTYRAC 2 cut(s) 559, 688
HindII GTYRAC 2 cut(s) 559, 688
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 2 cut(s) 419, 849
HpaII CCGG 3 cut(s) 191, 628, 719
HphI GGTGA 2 cut(s) 260, 733
Hpy166II GTNNAC 4 cut(s) 31, 431, 559, 688
Hpy188I TCNGA 6 cut(s) 66, 105, 113, 357, 412, 424
Hpy188III TCNNGA 5 cut(s) 91, 242, 296, 484, 533
Hpy8I GTNNAC 4 cut(s) 31, 431, 559, 688
Hpy99I CGWCG 4 cut(s) 10, 223, 257, 266
HpyAV CCTTC 3 cut(s) 284, 530, 615
HpyCH4III ACNGT 4 cut(s) 289, 454, 613, 889
HpyCH4IV ACGT 3 cut(s) 147, 218, 463
HpyCH4V TGCA 3 cut(s) 475, 715, 877
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 371
HpyF3I CTNAG 2 cut(s) 110, 301
HpySE526I ACGT 3 cut(s) 147, 218, 463
Hsp92I GRCGYC 3 cut(s) 5, 117, 252
Hsp92II CATG 4 cut(s) 638, 738, 859, 881
HspAI GCGC 2 cut(s) 117, 321
KasI GGCGCC 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 769
KspI CCGCGG 1 cut(s) 24
Kzo9I GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
Lsp1109I GCAGC 2 cut(s) 699, 774
LweI GCATC 1 cut(s) 361
MaeI CTAG 3 cut(s) 314, 336, 546
MaeII ACGT 3 cut(s) 147, 218, 463
MaeIII GTNAC 4 cut(s) 256, 448, 613, 883
MalI GATC 9 cut(s) 95, 107, 267, 440, 510, 633, 645, 666, 771
MbiI CCGCTC 1 cut(s) 230
MboI GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
MboII GAAGA 9 cut(s) 76, 79, 146, 156, 159, 162, 165, 399, 448
MflI RGATCY 3 cut(s) 105, 643, 664
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 3 cut(s) 500, 564, 675
Mly113I GGCGCC 1 cut(s) 117
MlyI GAGTC 1 cut(s) 428
Mph1103I ATGCAT 1 cut(s) 879
MroXI GAANNNNTTC 1 cut(s) 867
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 762
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 191, 628, 719
MspR9I CCNGG 3 cut(s) 17, 468, 629
MvaI CCWGG 2 cut(s) 17, 468
MvnI CGCG 2 cut(s) 23, 323
MwoI GCNNNNNNNGC 2 cut(s) 123, 371
NarI GGCGCC 1 cut(s) 117
NciI CCSGG 1 cut(s) 629
NdeI CATATG 1 cut(s) 763
NdeII GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
NlaIII CATG 4 cut(s) 638, 738, 859, 881
NlaIV GGNNCC 2 cut(s) 118, 359
NmuCI GTSAC 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 1 cut(s) 859
PceI AGGCCT 1 cut(s) 832
PcsI WCGNNNNNNNCGW 3 cut(s) 240, 249, 261
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 1 cut(s) 849
Pfl23II CGTACG 2 cut(s) 144, 324
PinAI ACCGGT 1 cut(s) 718
PkrI GCNGC 3 cut(s) 22, 714, 789
PleI GAGTC 1 cut(s) 427
PluTI GGCGCC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 15, 466
PspFI CCCAGC 2 cut(s) 374, 813
PspGI CCWGG 2 cut(s) 15, 466
PspLI CGTACG 2 cut(s) 144, 324
PspN4I GGNNCC 2 cut(s) 118, 359
PspPI GGNCC 3 cut(s) 13, 183, 357
PsuI RGATCY 3 cut(s) 105, 643, 664
RsaI GTAC 3 cut(s) 32, 146, 326
RsaNI GTAC 3 cut(s) 31, 145, 325
RseI CAYNNNNRTG 1 cut(s) 762
SacI GAGCTC 1 cut(s) 64
SacII CCGCGG 1 cut(s) 24
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 3 cut(s) 21, 713, 788
Sau3AI GATC 9 cut(s) 93, 105, 265, 438, 508, 631, 643, 664, 769
Sau96I GGNCC 3 cut(s) 13, 183, 357
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 3 cut(s) 17, 468, 629
SduI GDGCHC 1 cut(s) 64
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 1 cut(s) 609
SfoI GGCGCC 1 cut(s) 118
Sfr303I CCGCGG 1 cut(s) 24
SgrBI CCGCGG 1 cut(s) 24
SinI GGWCC 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 762
Sse9I AATT 3 cut(s) 500, 564, 675
SseBI AGGCCT 1 cut(s) 832
SsiI CCGC 4 cut(s) 21, 23, 228, 271
SspDI GGCGCC 1 cut(s) 116
SspMI CTAG 3 cut(s) 314, 336, 546
SstI GAGCTC 1 cut(s) 64
StuI AGGCCT 1 cut(s) 832
StyD4I CCNGG 3 cut(s) 15, 466, 627
TaaI ACNGT 4 cut(s) 289, 454, 613, 889
TaiI ACGT 3 cut(s) 150, 221, 466
TaqI TCGA 3 cut(s) 243, 659, 673
TasI AATT 3 cut(s) 500, 564, 675
TauI GCSGC 1 cut(s) 23
TfiI GAWTC 1 cut(s) 849
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 2 cut(s) 202, 459
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 2 cut(s) 712, 787
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 1 cut(s) 83
TspGWI ACGGA 3 cut(s) 383, 505, 635
TspRI CASTG 2 cut(s) 202, 459
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 1 cut(s) 500
XceI RCATGY 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 3 cut(s) 314, 336, 546
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.