Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005347

Physical Location & Seq

Genomic Coordinates Reverse (-)

26487555 .. 26491144

Gene Structure 3590 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1158100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

ATGGACGCCGACTCTTTCAACCCCAACAGCGCCTTGAGTGACACCCGCTTCTCCGACCTCAAGCCGCCGCTCTCCGAACCGGTTCTCGAAGCCCTATCCCAAGGCGGGTTCGAGTTCTGCACTCCCGTTCAAGCCGCCACAATCCCCTTACTATGCAGCTTCAAGGACGTGGCGGTGGACGCCGCCACTGGCTCAGGCAAAACCCTAGCCTTTGTGGTCCCGATGGTGGAGATACTCCGGCGAGTTTCAACCACTCCAAAACCTCAAGAGGTGATGGGAATGATTATATCTCCGACTAGGGAGCTGTCTTCGCAGATATACAATGTGGCGAAACCCTTCATTTCGACGCTGCCGAATTTCAAGTGCGTCCTTTTGGTTGGTGGAGGCCAAGTGAAATCCGACGTGAAACAAATCGAGGAGGAAGGAGCCAACTTGTTGATCGGCACGCCGGGAAGACTATTCGACATTATGGAACGCATTGATGGCTTGGATTTGCGGAACCTTGAGATTTTGATCTTAGATGAGGCCGATAGGCTATTGGATATGGGGTTCCAGAAGCAGTTAAATGATATTATGTCTCGATTACCGAAGCTTCGTAGAACTGGTCTGTTTTCAGCTACCCAAACTGAGGCAGTTGAAGAACTGGCCAGAGCAGGACTGAGGAATCCTGTGAGGGTTGAAGTACGAGCTGAAACAAAATCGAGTGATTCGGCGTTAAGGAAAAAATTAGCCTCTTCTAAAACACCTTCTGGCCTTGCCCTTGAGTATCTGGAGTGTGAGGGAGACAAAAAACCGTCACAGCTTGTGGATCTTCTTGTTAAGAATAAGTCTAACAAGACCATAGTATACTTCATGACTTGTGCTTGTGTTGACTACTGGGGACTTGTTCTTCCATTGCTTGGTCCTTTGAAGGGTTTCCCTTTGATTGCTCTACATGGGAAGATGAAGCAGGCCGCAAGGGACAAAGCTTTGGCCTCATTTACATCTCTCTCAAGTGGCGTCCTTCTGTGTACTGATGTTGCTGCGCGAGGGCTCGACATTCCAGGCGTTGACTGTATAGTGCAGTATGATACCCCTCAAGATCCAAATGTGTTCGTGCATAGAGTTGGCCGAACAGCTCGTATGGGTAGACAAGGAAGTGCGATTGTTTTTCTATTGCCAAAGGAGGAAGCTTATGTAGAATTCCTACGGATAAGAAGAGTTCCTCTACAGGAAAGGAAATGCTCTAATGATGTTGCTGATGTTGTTCCTCAGATCCGATCAGCTGCAAAAAAGGACCGCGATGTTATGGAAAAGGGGCTCAGAGCATTTGTTTCTTACATGCGTGCATATAAAGAACATCACTGCTCGTACATTTTCAGGTGGAAAGAACTTGAAGTCGGGAAGTTGGGCATGGGATTCGGACTACTGCAGCTCCCTGCAATGCCTGAGGTAAAGCACCACTCGCTTTCCACAGAGGGTTTCATCCCTGTTGAAGACATCAACTTGGAGGAGATCAAATTTAAGGATAAATCTCGCGAGAAGCAAAGAAAGAGGAATCTACAATCGAAGAAAGAAGCAAAACAGCAAGAACCGAAACCTCAAAAAACCAAAAAGATCCAAAATGCTTCAGATGCTGCAATGAGGAAGAAAACGGGTAAGCAGAGGCGTGCTATCCAGACAGCTGAAGATGAAGATGAGTTGGCAAGAGAATACCGCTTGCTAAAGAAGCTCAAAAAGGGGGCCATCGACGAAAGTGAATTTGCAAAGTTAACAGGAACAGAAGACTTGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003727 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008187 GO:0008266 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

592

Amino Acids

65.87

Weight (kDa)

9.07

Isoelectric Point (pI)

42.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	41 - 214	2.5e-42	DEAD/DEAH box helicase
Helicase_C	PF00271	264 - 376	2e-25	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	426 - 475	1.6e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G71280 AT1G71280 AT1G71370 AT5G05450
fragaria_vesca	FvH4_6g01590 FvH4_6g01590 FvH4_6g01590 FvH4_6g03480 FvH4_7g25130 FvH4_7g25130 FvH4_7g25130
malus_domestica	MD01G1158100.v1.1 MD07G1226800.v1.1
prunus_persica	Prupe.2G256400_v2.0.a1
pyrus_communis	pycom01g17480 pycom07g20540
rosa_chinensis	RchiOBHm_Chr1g0371021 RchiOBHm_Chr1g0371031
rosa_laevigata	RLG00000026991
rosa_multiflora	Rmu_co8450187.1_g000001 Rmu_sc0001018.1_g000027 Rmu_sc0003272.1_g000004
rosa_roxburghii	Rroxscaffold_4G00286030
rosa_rugosa	Rorug01G0360900
rosa_samantha	Rh1AG371200 Rh1BG334700 Rh1CG348000 Rh1DG365900
rosa_wichuraiana	Rw1G032650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	899
AccBSI	CCGCTC	1 cut(s)	70
AccI	GTMKAC	2 cut(s)	846, 1129
AccII	CGCG	3 cut(s)	1027, 1281, 1518
AclWI	GGATC	4 cut(s)	816, 1076, 1249, 1591
AcoI	YGGCCR	2 cut(s)	645, 1108
AcsI	RAATTY	4 cut(s)	355, 1181, 1499, 1739
AcuI	CTGAAG	2 cut(s)	1593, 1686
AcyI	GRCGYC	3 cut(s)	6, 180, 999
AfaI	GTAC	3 cut(s)	684, 1012, 1352
AfiI	CCNNNNNNNGG	5 cut(s)	105, 226, 628, 899, 911
AgeI	ACCGGT	1 cut(s)	79
AjiI	CACGTC	2 cut(s)	169, 403
AjnI	CCWGG	1 cut(s)	1042
AloI	GAACNNNNNNTCC	2 cut(s)	533, 565
Alw26I	GTCTC	2 cut(s)	582, 777
AlwI	GGATC	4 cut(s)	816, 1076, 1249, 1591
AlwNI	CAGNNNCTG	1 cut(s)	1616
AoxI	GGCC	8 cut(s)	385, 525, 645, 751, 951, 972, 1108, 1722
ApeKI	GCWGC	6 cut(s)	156, 349, 1022, 1265, 1411, 1616
ApoI	RAATTY	4 cut(s)	355, 1181, 1499, 1739
AsiGI	ACCGGT	1 cut(s)	79
Asp700I	GAANNNNTTC	3 cut(s)	81, 335, 914
AspLEI	GCGC	2 cut(s)	32, 1027
AspS9I	GGNCC	4 cut(s)	217, 902, 1276, 1722
AsuC2I	CCSGG	1 cut(s)	450
AsuHPI	GGTGA	1 cut(s)	283
AvaII	GGWCC	3 cut(s)	217, 902, 1276
AxyI	CCTNAGG	1 cut(s)	1428
BalI	TGGCCA	1 cut(s)	647
BanII	GRGCYC	2 cut(s)	1035, 1302
BbsI	GAAGAC	4 cut(s)	300, 460, 1482, 1770
BbvI	GCAGC	6 cut(s)	168, 336, 1009, 1252, 1423, 1603
BccI	CCATC	4 cut(s)	217, 268, 476, 1733
BcgI	CGANNNNNNTGC	2 cut(s)	1381, 1415
BciT130I	CCWGG	1 cut(s)	1044
BcnI	CCSGG	1 cut(s)	450
BcoDI	GTCTC	2 cut(s)	582, 777
BfaI	CTAG	2 cut(s)	206, 297
BfmI	CTRYAG	2 cut(s)	1208, 1409
BfoI	RGCGCY	1 cut(s)	33
Bme1390I	CCNGG	2 cut(s)	450, 1044
Bme18I	GGWCC	3 cut(s)	217, 902, 1276
BmgBI	CACGTC	2 cut(s)	169, 403
BmgT120I	GGNCC	4 cut(s)	217, 902, 1276, 1722
BmiI	GGNNCC	5 cut(s)	219, 427, 500, 551, 1723
BmrFI	CCNGG	2 cut(s)	450, 1044
BmrI	ACTGGG	1 cut(s)	886
BmsI	GCATC	1 cut(s)	1603
BmuI	ACTGGG	1 cut(s)	886
BpiI	GAAGAC	4 cut(s)	300, 460, 1482, 1770
BpmI	CTGGAG	1 cut(s)	791
Bpu10I	CCTNAGC	1 cut(s)	193
BpuEI	CTTGAG	7 cut(s)	44, 55, 249, 524, 782, 976, 1062
BpuMI	CCSGG	1 cut(s)	450
BsaBI	GATNNNNATC	1 cut(s)	512
BsaHI	GRCGYC	3 cut(s)	6, 180, 999
BsaJI	CCNNGG	1 cut(s)	100
BsaWI	WCCGGW	1 cut(s)	79
BsaXI	ACNNNNNCTCC	4 cut(s)	375, 405, 1398, 1428
Bsc4I	CCNNNNNNNGG	5 cut(s)	105, 226, 628, 899, 911
Bse118I	RCCGGY	1 cut(s)	79
Bse1I	ACTGG	4 cut(s)	193, 607, 648, 881
Bse21I	CCTNAGG	1 cut(s)	1428
Bse3DI	GCAATG	3 cut(s)	893, 1428, 1626
Bse8I	GATNNNNATC	1 cut(s)	512
BseBI	CCWGG	1 cut(s)	1044
BseDI	CCNNGG	1 cut(s)	100
BseGI	GGATG	1 cut(s)	1464
BseJI	GATNNNNATC	1 cut(s)	512
BseLI	CCNNNNNNNGG	5 cut(s)	105, 226, 628, 899, 911
BseMI	GCAATG	3 cut(s)	893, 1428, 1626
BseMII	CTCAG	6 cut(s)	207, 618, 650, 1265, 1315, 1419
BseNI	ACTGG	4 cut(s)	193, 607, 648, 881
BseRI	GAGGAG	2 cut(s)	431, 1505
BseXI	GCAGC	6 cut(s)	168, 336, 1009, 1252, 1423, 1603
BsgI	GTGCAG	2 cut(s)	103, 1082
Bsh1236I	CGCG	3 cut(s)	1027, 1281, 1518
BshFI	GGCC	8 cut(s)	387, 527, 647, 753, 953, 974, 1110, 1724
BshTI	ACCGGT	1 cut(s)	79
BsiSI	CCGG	3 cut(s)	80, 238, 449
BslFI	GGGAC	3 cut(s)	203, 894, 974
BslI	CCNNNNNNNGG	5 cut(s)	105, 226, 628, 899, 911
BsmAI	GTCTC	2 cut(s)	582, 777
BsmFI	GGGAC	3 cut(s)	203, 894, 974
BsnI	GGCC	8 cut(s)	387, 527, 647, 753, 953, 974, 1110, 1724
Bsp1286I	GDGCHC	2 cut(s)	1035, 1302
Bsp143I	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
Bsp68I	TCGCGA	1 cut(s)	1518
BspANI	GGCC	8 cut(s)	387, 527, 647, 753, 953, 974, 1110, 1724
BspCNI	CTCAG	6 cut(s)	206, 619, 651, 1264, 1314, 1420
BspFNI	CGCG	3 cut(s)	1027, 1281, 1518
BspHI	TCATGA	1 cut(s)	852
BspLI	GGNNCC	5 cut(s)	219, 427, 500, 551, 1723
BspMAI	CTGCAG	1 cut(s)	1413
BspPI	GGATC	4 cut(s)	816, 1076, 1249, 1591
BsrBI	CCGCTC	1 cut(s)	70
BsrDI	GCAATG	3 cut(s)	893, 1428, 1626
BsrFI	RCCGGY	1 cut(s)	79
BsrI	ACTGG	4 cut(s)	193, 607, 648, 881
BssAI	RCCGGY	1 cut(s)	79
BssECI	CCNNGG	1 cut(s)	100
BssMI	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
BssNAI	GTATAC	1 cut(s)	847
BssNI	GRCGYC	3 cut(s)	6, 180, 999
BssT1I	CCWWGG	1 cut(s)	100
Bst1107I	GTATAC	1 cut(s)	847
Bst2UI	CCWGG	1 cut(s)	1044
Bst4CI	ACNGT	2 cut(s)	795, 1055
Bst6I	CTCTTC	2 cut(s)	739, 1192
BstACI	GRCGYC	3 cut(s)	6, 180, 999
BstC8I	GCNNGC	5 cut(s)	446, 951, 1326, 1650, 1700
BstDEI	CTNAG	7 cut(s)	193, 517, 627, 659, 1251, 1301, 1428
BstF5I	GGATG	1 cut(s)	1464
BstFNI	CGCG	3 cut(s)	1027, 1281, 1518
BstH2I	RGCGCY	1 cut(s)	33
BstHHI	GCGC	2 cut(s)	32, 1027
BstKTI	GATC	8 cut(s)	441, 516, 811, 1084, 1257, 1262, 1497, 1599
BstMAI	GTCTC	2 cut(s)	582, 777
BstMBI	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
BstMWI	GCNNNNNNNGC	6 cut(s)	179, 310, 483, 1444, 1613, 1708
BstNI	CCWGG	1 cut(s)	1044
BstNSI	RCATGY	1 cut(s)	1324
BstSCI	CCNGG	2 cut(s)	448, 1042
BstSFI	CTRYAG	2 cut(s)	1208, 1409
BstUI	CGCG	3 cut(s)	1027, 1281, 1518
BstV1I	GCAGC	6 cut(s)	168, 336, 1009, 1252, 1423, 1603
BstV2I	GAAGAC	4 cut(s)	300, 460, 1482, 1770
BstX2I	RGATCY	4 cut(s)	808, 1081, 1254, 1596
BstYI	RGATCY	4 cut(s)	808, 1081, 1254, 1596
BstZ17I	GTATAC	1 cut(s)	847
Bsu36I	CCTNAGG	1 cut(s)	1428
BsuRI	GGCC	8 cut(s)	387, 527, 647, 753, 953, 974, 1110, 1724
BtgZI	GCGATG	1 cut(s)	1296
BtrI	CACGTC	2 cut(s)	169, 403
BtsCI	GGATG	1 cut(s)	1464
BtsI	GCAGTG	1 cut(s)	1342
BtsIMutI	CAGTG	2 cut(s)	186, 1342
BtuMI	TCGCGA	1 cut(s)	1518
Cac8I	GCNNGC	5 cut(s)	446, 951, 1326, 1650, 1700
CaiI	CAGNNNCTG	1 cut(s)	1616
CciI	TCATGA	1 cut(s)	852
CfoI	GCGC	2 cut(s)	32, 1027
Cfr10I	RCCGGY	1 cut(s)	79
Cfr13I	GGNCC	4 cut(s)	217, 902, 1276, 1722
CseI	GACGC	5 cut(s)	14, 188, 355, 355, 988
Csp6I	GTAC	3 cut(s)	683, 1011, 1351
CspAI	ACCGGT	1 cut(s)	79
CviAII	CATG	4 cut(s)	853, 935, 1321, 1393
CviQI	GTAC	3 cut(s)	683, 1011, 1351
DdeI	CTNAG	7 cut(s)	193, 517, 627, 659, 1251, 1301, 1428
DpnI	GATC	8 cut(s)	440, 515, 810, 1083, 1256, 1261, 1496, 1598
DpnII	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
EaeI	YGGCCR	2 cut(s)	645, 1108
Eam1104I	CTCTTC	2 cut(s)	739, 1192
EarI	CTCTTC	2 cut(s)	739, 1192
Eco130I	CCWWGG	1 cut(s)	100
Eco24I	GRGCYC	2 cut(s)	1035, 1302
Eco47I	GGWCC	3 cut(s)	217, 902, 1276
Eco57I	CTGAAG	2 cut(s)	1593, 1686
Eco81I	CCTNAGG	1 cut(s)	1428
EcoRI	GAATTC	1 cut(s)	1181
EcoRII	CCWGG	1 cut(s)	1042
EcoT14I	CCWWGG	1 cut(s)	100
EcoT38I	GRGCYC	2 cut(s)	1035, 1302
ErhI	CCWWGG	1 cut(s)	100
FaeI	CATG	4 cut(s)	856, 938, 1324, 1396
FaqI	GGGAC	3 cut(s)	203, 894, 974
FatI	CATG	4 cut(s)	852, 934, 1320, 1392
FauI	CCCGC	2 cut(s)	53, 98
FblI	GTMKAC	2 cut(s)	846, 1129
FokI	GGATG	1 cut(s)	1451
FriOI	GRGCYC	2 cut(s)	1035, 1302
FspBI	CTAG	2 cut(s)	206, 297
GlaI	GCGC	2 cut(s)	31, 1026
GsuI	CTGGAG	1 cut(s)	791
HaeII	RGCGCY	1 cut(s)	33
HaeIII	GGCC	8 cut(s)	387, 527, 647, 753, 953, 974, 1110, 1724
HapII	CCGG	3 cut(s)	80, 238, 449
HgaI	GACGC	5 cut(s)	14, 188, 355, 355, 988
HhaI	GCGC	2 cut(s)	32, 1027
Hin1I	GRCGYC	3 cut(s)	6, 180, 999
Hin1II	CATG	4 cut(s)	856, 938, 1324, 1396
Hin6I	GCGC	2 cut(s)	30, 1025
HinP1I	GCGC	2 cut(s)	30, 1025
HincII	GTYRAC	3 cut(s)	871, 1051, 1752
HindII	GTYRAC	3 cut(s)	871, 1051, 1752
HindIII	AAGCTT	3 cut(s)	590, 966, 1170
HinfI	GANTC	5 cut(s)	11, 664, 707, 1398, 1537
HpaI	GTTAAC	1 cut(s)	1752
HpaII	CCGG	3 cut(s)	80, 238, 449
HphI	GGTGA	1 cut(s)	283
Hpy166II	GTNNAC	7 cut(s)	178, 847, 871, 1011, 1051, 1130, 1752
Hpy188I	TCNGA	9 cut(s)	55, 76, 294, 400, 1254, 1259, 1304, 1403, 1612
Hpy8I	GTNNAC	7 cut(s)	178, 847, 871, 1011, 1051, 1130, 1752
Hpy99I	CGWCG	3 cut(s)	349, 404, 1733
HpyAV	CCTTC	5 cut(s)	346, 416, 756, 904, 1013
HpyCH4III	ACNGT	2 cut(s)	795, 1055
HpyCH4IV	ACGT	2 cut(s)	168, 402
HpyF10VI	GCNNNNNNNGC	6 cut(s)	179, 310, 483, 1444, 1613, 1708
HpyF3I	CTNAG	7 cut(s)	193, 517, 627, 659, 1251, 1301, 1428
HpySE526I	ACGT	2 cut(s)	168, 402
Hsp92I	GRCGYC	3 cut(s)	6, 180, 999
Hsp92II	CATG	4 cut(s)	856, 938, 1324, 1396
HspAI	GCGC	2 cut(s)	30, 1025
KspAI	GTTAAC	1 cut(s)	1752
Kzo9I	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
LmnI	GCTCC	3 cut(s)	301, 425, 1419
Lsp1109I	GCAGC	6 cut(s)	168, 336, 1009, 1252, 1423, 1603
LweI	GCATC	1 cut(s)	1603
MaeI	CTAG	2 cut(s)	206, 297
MaeII	ACGT	2 cut(s)	168, 402
MaeIII	GTNAC	2 cut(s)	38, 795
MalI	GATC	8 cut(s)	440, 515, 810, 1083, 1256, 1261, 1496, 1598
MbiI	CCGCTC	1 cut(s)	70
MboI	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
MflI	RGATCY	4 cut(s)	808, 1081, 1254, 1596
MhlI	GDGCHC	2 cut(s)	1035, 1302
MlsI	TGGCCA	1 cut(s)	647
MluCI	AATT	5 cut(s)	355, 725, 1181, 1499, 1739
MluNI	TGGCCA	1 cut(s)	647
MlyI	GAGTC	1 cut(s)	5
MmeI	TCCRAC	3 cut(s)	78, 317, 423
Mox20I	TGGCCA	1 cut(s)	647
MroXI	GAANNNNTTC	3 cut(s)	81, 335, 914
MscI	TGGCCA	1 cut(s)	647
MseI	TTAA	5 cut(s)	563, 716, 819, 1503, 1751
Msp20I	TGGCCA	1 cut(s)	647
MspA1I	CMGCKG	2 cut(s)	1265, 1664
MspI	CCGG	3 cut(s)	80, 238, 449
MspR9I	CCNGG	2 cut(s)	450, 1044
MvaI	CCWGG	1 cut(s)	1044
MvnI	CGCG	3 cut(s)	1027, 1281, 1518
MwoI	GCNNNNNNNGC	6 cut(s)	179, 310, 483, 1444, 1613, 1708
NciI	CCSGG	1 cut(s)	450
NdeII	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
NlaIII	CATG	4 cut(s)	856, 938, 1324, 1396
NlaIV	GGNNCC	5 cut(s)	219, 427, 500, 551, 1723
NmuCI	GTSAC	2 cut(s)	38, 795
NruI	TCGCGA	1 cut(s)	1518
NspI	RCATGY	1 cut(s)	1324
PagI	TCATGA	1 cut(s)	852
PcsI	WCGNNNNNNNCGW	1 cut(s)	350
PdmI	GAANNNNTTC	3 cut(s)	81, 335, 914
PfeI	GAWTC	4 cut(s)	664, 707, 1398, 1537
PflMI	CCANNNNNTGG	1 cut(s)	899
PinAI	ACCGGT	1 cut(s)	79
PleI	GAGTC	1 cut(s)	5
PpsI	GAGTC	1 cut(s)	5
Psp6I	CCWGG	1 cut(s)	1042
PspGI	CCWGG	1 cut(s)	1042
PspN4I	GGNNCC	5 cut(s)	219, 427, 500, 551, 1723
PspPI	GGNCC	4 cut(s)	217, 902, 1276, 1722
PstI	CTGCAG	1 cut(s)	1413
PstNI	CAGNNNCTG	1 cut(s)	1616
PsuI	RGATCY	4 cut(s)	808, 1081, 1254, 1596
PvuII	CAGCTG	2 cut(s)	1265, 1664
RruI	TCGCGA	1 cut(s)	1518
RsaI	GTAC	3 cut(s)	684, 1012, 1352
RsaNI	GTAC	3 cut(s)	683, 1011, 1351
SaqAI	TTAA	5 cut(s)	563, 716, 819, 1503, 1751
Sau3AI	GATC	8 cut(s)	438, 513, 808, 1081, 1254, 1259, 1494, 1596
Sau96I	GGNCC	4 cut(s)	217, 902, 1276, 1722
SchI	GAGTC	1 cut(s)	5
ScrFI	CCNGG	2 cut(s)	450, 1044
SduI	GDGCHC	2 cut(s)	1035, 1302
SfaNI	GCATC	1 cut(s)	1603
SfcI	CTRYAG	2 cut(s)	1208, 1409
SinI	GGWCC	3 cut(s)	217, 902, 1276
SmlI	CTYRAG	7 cut(s)	34, 59, 264, 503, 761, 991, 1077
SmoI	CTYRAG	7 cut(s)	34, 59, 264, 503, 761, 991, 1077
Sse9I	AATT	5 cut(s)	355, 725, 1181, 1499, 1739
SspMI	CTAG	2 cut(s)	206, 297
StyD4I	CCNGG	2 cut(s)	448, 1042
StyI	CCWWGG	1 cut(s)	100
TaaI	ACNGT	2 cut(s)	795, 1055
TaiI	ACGT	2 cut(s)	171, 405
TasI	AATT	5 cut(s)	355, 725, 1181, 1499, 1739
TatI	WGTACW	1 cut(s)	1010
TauI	GCSGC	5 cut(s)	67, 70, 137, 185, 956
TfiI	GAWTC	4 cut(s)	664, 707, 1398, 1537
Tru1I	TTAA	5 cut(s)	563, 716, 819, 1503, 1751
Tru9I	TTAA	5 cut(s)	563, 716, 819, 1503, 1751
TscAI	CASTG	2 cut(s)	193, 1349
TseFI	GTSAC	2 cut(s)	38, 795
TseI	GCWGC	6 cut(s)	156, 349, 1022, 1265, 1411, 1616
Tsp45I	GTSAC	2 cut(s)	38, 795
TspDTI	ATGAA	5 cut(s)	328, 841, 959, 1453, 1686
TspGWI	ACGGA	1 cut(s)	1204
TspRI	CASTG	2 cut(s)	193, 1349
Van91I	CCANNNNNTGG	1 cut(s)	899
VpaK11BI	GGWCC	3 cut(s)	217, 902, 1276
XapI	RAATTY	4 cut(s)	355, 1181, 1499, 1739
XceI	RCATGY	1 cut(s)	1324
XmiI	GTMKAC	2 cut(s)	846, 1129
XmnI	GAANNNNTTC	3 cut(s)	81, 335, 914
XspI	CTAG	2 cut(s)	206, 297

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.