MD01G1162700.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007679
Physical Location & Seq
Forward (+)
26756983 .. 26758929
1947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1162700.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCTTCCTCTTCTTTCATGGCCACTTTTACCTTGGCCTTCCTTGTGCTCATGAGCACCACCAGTGCTCAACTTTCAACAAATTTCTACTCTAGTTCTTGCCCTAGGCTCTTCTCAACCGTGAAATCCACCGTGCAATCCGCTATACAAAAAGAGGCCCGAATGGGTGCCTCTCTTCTTCGGCTCCACTTCCATGACTGTTTTGTTAATGGATGTGACGGGTCGCTGCTCCTTGACGACACATCGAGCTTTACCGGAGAGAAAAATGCAGCTCCTAATCGGAATTCTGCACGGGGATTTGACGTGGTTGACAACATAAAGTCCGCAGTGGAAAACGTGTGCCCCGGCGTGGTGTCATGTGCTGATATATTAGCCATTGCTGCCAGAGACTCCGTTGTCACTCTTGGAGGGCCAAGTTGGAACGTTAAGTTGGGAAGAAGAGACGCAAGGACTGCAAGCCAAGCCGCTGCTAACAACAACATCCCCCCTCCAACCCGAAACCTAAACCAGCTCATCTCTACATTCAGTGCTGTTGGTCTTTCCACCAGAGACTTGGTTGCTTTATCCGGATCCCATACAATTGGGCAAGCAAGGTGCACATCATTCAGACCCCGCATATACAACGAGACCAATCTGGATAGTTCCTTGGCTCAAACAAGGCGATCCAACTGTCCAAGAACCTCTGGCTCAGGAGATAACAATTTGGCTCCACTTGACCTTCAAACCCCTACTTCTTTCGACAACAACTACTTCAAAAACCTAATCCAGAATAAAGGTCTCCTCCACTCTGACCAGCAGCTCTTCAATGGTGGTTCCACCGATTCGATAGTGAGAGCCTACAGCAACAGCTACAACGCCTTCAGTTCTGACTTTGCGAGTGCTATGATCAAGATGGGAGACATCAAACCTCTCACTGGATCCAGTGGGGAGATTAGGAAGAATTGTAGGAAGACCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

320

Amino Acids

34.24

Weight (kDa)

9.19

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 282 6.1e-89 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 167
AccIII TCCGGA 1 cut(s) 566
AciI CCGC 4 cut(s) 141, 324, 465, 613
AclI AACGTT 1 cut(s) 423
AclWI GGATC 5 cut(s) 564, 577, 657, 912, 925
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 2 cut(s) 82, 283
AcuI CTGAAG 1 cut(s) 844
AfiI CCNNNNNNNGG 2 cut(s) 349, 914
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 4 cut(s) 78, 722, 754, 805
AjiI CACGTC 1 cut(s) 304
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AluBI AGCT 5 cut(s) 249, 272, 511, 799, 849
AluI AGCT 5 cut(s) 249, 272, 511, 799, 849
Alw21I GWGCWC 4 cut(s) 51, 59, 70, 599
Alw26I GTCTC 6 cut(s) 381, 435, 543, 620, 782, 891
Alw44I GTGCAC 1 cut(s) 595
AlwI GGATC 5 cut(s) 564, 577, 657, 912, 925
Aor13HI TCCGGA 1 cut(s) 566
AoxI GGCC 4 cut(s) 21, 36, 156, 410
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 5 cut(s) 226, 269, 380, 467, 796
ApoI RAATTY 2 cut(s) 82, 283
AspA2I CCTAGG 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 157, 410
AsuC2I CCSGG 1 cut(s) 345
AvrII CCTAGG 1 cut(s) 104
BaeGI GKGCMC 2 cut(s) 344, 599
BalI TGGCCA 1 cut(s) 23
BamHI GGATCC 2 cut(s) 569, 917
BanI GGYRCC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 956
Bbv12I GWGCWC 4 cut(s) 51, 59, 70, 599
BbvI GCAGC 5 cut(s) 213, 281, 367, 454, 808
BccI CCATC 1 cut(s) 886
BclI TGATCA 1 cut(s) 885
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 6 cut(s) 381, 435, 543, 620, 782, 891
BfaI CTAG 2 cut(s) 93, 105
BfmI CTRYAG 1 cut(s) 838
BisI GCNGC 6 cut(s) 227, 270, 381, 465, 468, 797
BlnI CCTAGG 1 cut(s) 104
BlsI GCNGC 6 cut(s) 228, 271, 382, 466, 469, 798
Bme1390I CCNGG 1 cut(s) 345
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 2 cut(s) 157, 410
BmiI GGNNCC 6 cut(s) 169, 185, 571, 708, 814, 919
BmrFI CCNGG 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 688
BpuMI CCSGG 1 cut(s) 345
BsaI GGTCTC 2 cut(s) 620, 782
BsaJI CCNNGG 4 cut(s) 33, 104, 343, 645
BsaWI WCCGGW 2 cut(s) 254, 566
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 914
Bse1I ACTGG 3 cut(s) 63, 919, 921
Bse3DI GCAATG 1 cut(s) 375
BseAI TCCGGA 1 cut(s) 566
BseDI CCNNGG 4 cut(s) 33, 104, 343, 645
BseGI GGATG 2 cut(s) 218, 480
BseLI CCNNNNNNNGG 2 cut(s) 349, 914
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 702
BseNI ACTGG 3 cut(s) 63, 919, 921
BseRI GAGGAG 1 cut(s) 770
BseSI GKGCMC 2 cut(s) 344, 599
BseXI GCAGC 5 cut(s) 213, 281, 367, 454, 808
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 4 cut(s) 23, 38, 158, 412
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 4 cut(s) 51, 59, 70, 599
BsiSI CCGG 3 cut(s) 255, 345, 567
BslI CCNNNNNNNGG 2 cut(s) 349, 914
BsmAI GTCTC 6 cut(s) 381, 435, 543, 620, 782, 891
BsmBI CGTCTC 1 cut(s) 435
BsnI GGCC 4 cut(s) 23, 38, 158, 412
Bso31I GGTCTC 2 cut(s) 620, 782
Bsp1286I GDGCHC 5 cut(s) 51, 59, 70, 344, 599
Bsp13I TCCGGA 1 cut(s) 566
Bsp143I GATC 4 cut(s) 569, 662, 885, 917
BspACI CCGC 4 cut(s) 141, 324, 465, 613
BspANI GGCC 4 cut(s) 23, 38, 158, 412
BspCNI CTCAG 1 cut(s) 701
BspEI TCCGGA 1 cut(s) 566
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 6 cut(s) 169, 185, 571, 708, 814, 919
BspPI GGATC 5 cut(s) 564, 577, 657, 912, 925
BspQI GCTCTTC 2 cut(s) 116, 806
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 2 cut(s) 620, 782
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 3 cut(s) 63, 919, 921
BssECI CCNNGG 4 cut(s) 33, 104, 343, 645
BssMI GATC 4 cut(s) 569, 662, 885, 917
BssT1I CCWWGG 3 cut(s) 33, 104, 645
Bst4CI ACNGT 4 cut(s) 121, 133, 200, 671
Bst6I CTCTTC 5 cut(s) 16, 116, 180, 433, 806
BstAPI GCANNNNNTGC 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 457, 588
BstDEI CTNAG 1 cut(s) 688
BstF5I GGATG 2 cut(s) 218, 480
BstKTI GATC 4 cut(s) 572, 665, 888, 920
BstMAI GTCTC 6 cut(s) 381, 435, 543, 620, 782, 891
BstMBI GATC 4 cut(s) 569, 662, 885, 917
BstMWI GCNNNNNNNGC 3 cut(s) 380, 452, 461
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 838
BstSLI GKGCMC 2 cut(s) 344, 599
BstV1I GCAGC 5 cut(s) 213, 281, 367, 454, 808
BstV2I GAAGAC 1 cut(s) 956
BstX2I RGATCY 2 cut(s) 569, 917
BstXI CCANNNNNNTGG 2 cut(s) 553, 581
BstYI RGATCY 2 cut(s) 569, 917
BsuRI GGCC 4 cut(s) 23, 38, 158, 412
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 2 cut(s) 218, 480
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 5 cut(s) 70, 333, 532, 912, 928
Cac8I GCNNGC 2 cut(s) 457, 588
CciI TCATGA 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 157, 410
CseI GACGC 1 cut(s) 452
CviAII CATG 4 cut(s) 19, 52, 194, 357
DdeI CTNAG 1 cut(s) 688
DpnI GATC 4 cut(s) 571, 664, 887, 919
DpnII GATC 4 cut(s) 569, 662, 885, 917
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 5 cut(s) 16, 116, 180, 433, 806
EarI CTCTTC 5 cut(s) 16, 116, 180, 433, 806
Eco130I CCWWGG 3 cut(s) 33, 104, 645
Eco31I GGTCTC 2 cut(s) 620, 782
Eco57I CTGAAG 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 283
EcoT14I CCWWGG 3 cut(s) 33, 104, 645
ErhI CCWWGG 3 cut(s) 33, 104, 645
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 4 cut(s) 22, 55, 197, 360
FatI CATG 4 cut(s) 18, 51, 193, 356
FauI CCCGC 1 cut(s) 620
FbaI TGATCA 1 cut(s) 885
Fnu4HI GCNGC 6 cut(s) 227, 270, 381, 465, 468, 797
FokI GGATG 2 cut(s) 225, 467
Fsp4HI GCNGC 6 cut(s) 227, 270, 381, 465, 468, 797
FspBI CTAG 2 cut(s) 93, 105
GluI GCNGC 6 cut(s) 227, 270, 381, 465, 468, 797
HaeIII GGCC 4 cut(s) 23, 38, 158, 412
HapII CCGG 3 cut(s) 255, 345, 567
HgaI GACGC 1 cut(s) 452
Hin1II CATG 4 cut(s) 22, 55, 197, 360
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 2 cut(s) 389, 821
HpaII CCGG 3 cut(s) 255, 345, 567
Hpy166II GTNNAC 2 cut(s) 310, 597
Hpy188I TCNGA 4 cut(s) 282, 608, 790, 868
Hpy188III TCNNGA 6 cut(s) 52, 567, 635, 690, 766, 889
Hpy8I GTNNAC 2 cut(s) 310, 597
HpyAV CCTTC 3 cut(s) 49, 728, 868
HpyCH4III ACNGT 4 cut(s) 121, 133, 200, 671
HpyCH4IV ACGT 3 cut(s) 303, 336, 423
HpyCH4V TGCA 5 cut(s) 136, 269, 290, 455, 597
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 452, 461
HpyF3I CTNAG 1 cut(s) 688
HpySE526I ACGT 3 cut(s) 303, 336, 423
Hsp92II CATG 4 cut(s) 22, 55, 197, 360
Kpn2I TCCGGA 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 885
Kzo9I GATC 4 cut(s) 569, 662, 885, 917
LguI GCTCTTC 2 cut(s) 116, 806
LmnI GCTCC 4 cut(s) 189, 234, 277, 712
Lsp1109I GCAGC 5 cut(s) 213, 281, 367, 454, 808
MaeI CTAG 2 cut(s) 93, 105
MaeII ACGT 3 cut(s) 303, 336, 423
MaeIII GTNAC 2 cut(s) 215, 397
MalI GATC 4 cut(s) 571, 664, 887, 919
MboI GATC 4 cut(s) 569, 662, 885, 917
MboII GAAGA 8 cut(s) 3, 103, 167, 170, 447, 450, 793, 949
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 2 cut(s) 569, 917
MhlI GDGCHC 5 cut(s) 51, 59, 70, 344, 599
MlsI TGGCCA 1 cut(s) 23
MluCI AATT 5 cut(s) 82, 283, 579, 700, 940
MluNI TGGCCA 1 cut(s) 23
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 3 cut(s) 398, 515, 690
MnlI CCTC 8 cut(s) 19, 148, 181, 401, 498, 691, 791, 918
Mox20I TGGCCA 1 cut(s) 23
MroI TCCGGA 1 cut(s) 566
MscI TGGCCA 1 cut(s) 23
MseI TTAA 2 cut(s) 207, 426
MslI CAYNNNNRTG 1 cut(s) 192
Msp20I TGGCCA 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 3 cut(s) 255, 345, 567
MspR9I CCNGG 1 cut(s) 345
MunI CAATTG 1 cut(s) 579
MwoI GCNNNNNNNGC 3 cut(s) 380, 452, 461
NciI CCSGG 1 cut(s) 345
NdeII GATC 4 cut(s) 569, 662, 885, 917
NlaIII CATG 4 cut(s) 22, 55, 197, 360
NlaIV GGNNCC 6 cut(s) 169, 185, 571, 708, 814, 919
NmuCI GTSAC 2 cut(s) 215, 397
PagI TCATGA 1 cut(s) 51
PciSI GCTCTTC 2 cut(s) 116, 806
PfeI GAWTC 1 cut(s) 821
PkrI GCNGC 6 cut(s) 228, 271, 382, 466, 469, 798
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
Psp1406I AACGTT 1 cut(s) 423
PspN4I GGNNCC 6 cut(s) 169, 185, 571, 708, 814, 919
PspPI GGNCC 2 cut(s) 157, 410
PsuI RGATCY 2 cut(s) 569, 917
RseI CAYNNNNRTG 1 cut(s) 192
SapI GCTCTTC 2 cut(s) 116, 806
SaqAI TTAA 2 cut(s) 207, 426
SatI GCNGC 6 cut(s) 227, 270, 381, 465, 468, 797
Sau3AI GATC 4 cut(s) 569, 662, 885, 917
Sau96I GGNCC 2 cut(s) 157, 410
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 5 cut(s) 51, 59, 70, 344, 599
SfcI CTRYAG 1 cut(s) 838
SmiMI CAYNNNNRTG 1 cut(s) 192
Sse9I AATT 5 cut(s) 82, 283, 579, 700, 940
SsiI CCGC 4 cut(s) 141, 324, 465, 613
SspMI CTAG 2 cut(s) 93, 105
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 3 cut(s) 33, 104, 645
TaaI ACNGT 4 cut(s) 121, 133, 200, 671
TaiI ACGT 3 cut(s) 306, 339, 426
TaqI TCGA 3 cut(s) 245, 738, 824
TasI AATT 5 cut(s) 82, 283, 579, 700, 940
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 1 cut(s) 821
Tru1I TTAA 2 cut(s) 207, 426
Tru9I TTAA 2 cut(s) 207, 426
TscAI CASTG 5 cut(s) 70, 333, 532, 919, 928
TseFI GTSAC 2 cut(s) 215, 397
TseI GCWGC 5 cut(s) 226, 269, 380, 467, 796
Tsp45I GTSAC 2 cut(s) 215, 397
TspDTI ATGAA 1 cut(s) 7
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 5 cut(s) 70, 333, 532, 919, 928
VneI GTGCAC 1 cut(s) 595
XapI RAATTY 2 cut(s) 82, 283
XcmI CCANNNNNNNNNTGG 2 cut(s) 31, 550
XmaJI CCTAGG 1 cut(s) 104
XspI CTAG 2 cut(s) 93, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.