MD07G1230700.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007679
Physical Location & Seq
Forward (+)
30507239 .. 30509107
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1230700.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCTTCCTCTTCTGTCATGGCCATTTTGACCTTGGCTTTCCTTATGCTTATGAGAACTACTAACGCTCAACTTTCAACAAAATTTTACTCTAGCTCCTGCCCAAAGCTCTTCTCCACTGTGAAATCCACCGTGCATTCCGCTATACAAAAAGAGACTCGAATGGGTGCCTCTCTTCTTCGTCTCCACTTTCACGACTGTTTTGTTAATGGATGTGACGGATCGTTGCTCCTTGACGACACATCGAGCTTTACTGGAGAGAAAAATGCAGTTCCTAATAAGAATTCTGCGCGGGGATTTAACGTGGTTGACAAAATAAAGTCCGCAGTAGAAAACGCGTGCCCTGGCGTGGTGTCATGTGCTGATATATTGGCCATTGCTGCCAGAGAATCAGTTACCGTTCTTGGAGGGCCAAGTTGGGACGTTAAGTTGGGAAGAAGAGACACAAGGACTGCAAGCCAAGCTGCTGCTAACAACAGCATCCCCCCTCCAACCCGAAACCTAAACCAGCTCGTCTCTACATTCAATGCTCTTGGCCTTTCCACCAGAGACTTGGTTGCTTTATCCGGATCCCATACAATTGGGCAAGCAAGGTGCACAACATTCAGGCCTCGCATATACAACGAGACCAATCTGGACAGTTCCTTGGCTCAAACAAGGCAATCCAACTGTCCAAGAACCTCTGGCTCAGGAGACAACAATTTGGCTCCACTTGATCTGAAAAGCCCTACTTTTTTTGACAACAGCTACTTCAAAAACCTAATCCAGAACAAAGGTCTCCTCCATTCTGACCAGCAGCTCTTCAATGGTGGTTCCACCGATTCAATAGTGCGAGCCTACAGCAACAGCTACAACACCTTCAGTTCTGACTTTGCGAAGGCCATGATCAAGATGGGGGACATCAAACCTCTTACTGGATCCAATGGGGAGATTAGGAAGAATTGTAGGAAGCCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

320

Amino Acids

34.45

Weight (kDa)

9.47

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 282 8.4e-87 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccII CGCG 2 cut(s) 292, 338
AccIII TCCGGA 1 cut(s) 566
AciI CCGC 3 cut(s) 141, 292, 324
AclWI GGATC 5 cut(s) 229, 564, 577, 912, 925
AcoI YGGCCR 2 cut(s) 21, 372
AcsI RAATTY 2 cut(s) 83, 283
AcuI CTGAAG 1 cut(s) 844
AfiI CCNNNNNNNGG 2 cut(s) 349, 914
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 5 cut(s) 78, 526, 754, 805, 825
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 8 cut(s) 96, 109, 249, 464, 511, 747, 799, 849
AluI AGCT 8 cut(s) 96, 109, 249, 464, 511, 747, 799, 849
Alw21I GWGCWC 1 cut(s) 599
Alw26I GTCTC 8 cut(s) 149, 188, 435, 520, 543, 620, 687, 782
Alw44I GTGCAC 1 cut(s) 595
AlwI GGATC 5 cut(s) 229, 564, 577, 912, 925
Aor13HI TCCGGA 1 cut(s) 566
AoxI GGCC 6 cut(s) 21, 372, 410, 535, 608, 879
ApaLI GTGCAC 1 cut(s) 595
ApeKI GCWGC 4 cut(s) 380, 464, 467, 796
ApoI RAATTY 2 cut(s) 83, 283
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 410
BaeGI GKGCMC 2 cut(s) 344, 599
BalI TGGCCA 2 cut(s) 23, 374
BamHI GGATCC 2 cut(s) 569, 917
BanI GGYRCC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 599
BbvI GCAGC 4 cut(s) 367, 451, 454, 808
BccI CCATC 1 cut(s) 886
BciT130I CCWGG 1 cut(s) 345
BclI TGATCA 1 cut(s) 885
BcoDI GTCTC 8 cut(s) 149, 188, 435, 520, 543, 620, 687, 782
BfaI CTAG 1 cut(s) 93
BfmI CTRYAG 1 cut(s) 838
BisI GCNGC 4 cut(s) 381, 465, 468, 797
BlsI GCNGC 4 cut(s) 382, 466, 469, 798
Bme1390I CCNGG 1 cut(s) 345
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 5 cut(s) 169, 571, 708, 814, 919
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 489
BpmI CTGGAG 1 cut(s) 276
Bpu10I CCTNAGC 1 cut(s) 688
BsaI GGTCTC 2 cut(s) 620, 782
BsaJI CCNNGG 3 cut(s) 33, 343, 645
BsaWI WCCGGW 1 cut(s) 566
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 914
Bse1I ACTGG 2 cut(s) 259, 919
Bse3DI GCAATG 1 cut(s) 375
BseAI TCCGGA 1 cut(s) 566
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 3 cut(s) 33, 343, 645
BseGI GGATG 2 cut(s) 218, 480
BseLI CCNNNNNNNGG 2 cut(s) 349, 914
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 702
BseNI ACTGG 2 cut(s) 259, 919
BseRI GAGGAG 1 cut(s) 770
BseSI GKGCMC 2 cut(s) 344, 599
BseXI GCAGC 4 cut(s) 367, 451, 454, 808
Bsh1236I CGCG 2 cut(s) 292, 338
BshFI GGCC 6 cut(s) 23, 374, 412, 537, 610, 881
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 599
BsiSI CCGG 1 cut(s) 567
BslFI GGGAC 2 cut(s) 434, 911
BslI CCNNNNNNNGG 2 cut(s) 349, 914
BsmAI GTCTC 8 cut(s) 149, 188, 435, 520, 543, 620, 687, 782
BsmBI CGTCTC 2 cut(s) 188, 520
BsmFI GGGAC 2 cut(s) 434, 911
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 6 cut(s) 23, 374, 412, 537, 610, 881
Bso31I GGTCTC 2 cut(s) 620, 782
Bsp1286I GDGCHC 2 cut(s) 344, 599
Bsp13I TCCGGA 1 cut(s) 566
Bsp143I GATC 5 cut(s) 221, 569, 715, 885, 917
BspACI CCGC 3 cut(s) 141, 292, 324
BspANI GGCC 6 cut(s) 23, 374, 412, 537, 610, 881
BspCNI CTCAG 1 cut(s) 701
BspEI TCCGGA 1 cut(s) 566
BspFNI CGCG 2 cut(s) 292, 338
BspLI GGNNCC 5 cut(s) 169, 571, 708, 814, 919
BspPI GGATC 5 cut(s) 229, 564, 577, 912, 925
BspQI GCTCTTC 2 cut(s) 116, 806
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 2 cut(s) 620, 782
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 2 cut(s) 259, 919
BssECI CCNNGG 3 cut(s) 33, 343, 645
BssMI GATC 5 cut(s) 221, 569, 715, 885, 917
BssT1I CCWWGG 2 cut(s) 33, 645
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 6 cut(s) 121, 133, 200, 400, 641, 671
Bst6I CTCTTC 5 cut(s) 16, 116, 180, 433, 806
BstC8I GCNNGC 4 cut(s) 340, 457, 588, 834
BstDEI CTNAG 1 cut(s) 688
BstF5I GGATG 2 cut(s) 218, 480
BstFNI CGCG 2 cut(s) 292, 338
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 5 cut(s) 224, 572, 718, 888, 920
BstMAI GTCTC 8 cut(s) 149, 188, 435, 520, 543, 620, 687, 782
BstMBI GATC 5 cut(s) 221, 569, 715, 885, 917
BstMWI GCNNNNNNNGC 2 cut(s) 380, 461
BstNI CCWGG 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 838
BstSLI GKGCMC 2 cut(s) 344, 599
BstUI CGCG 2 cut(s) 292, 338
BstV1I GCAGC 4 cut(s) 367, 451, 454, 808
BstX2I RGATCY 2 cut(s) 569, 917
BstXI CCANNNNNNTGG 2 cut(s) 553, 581
BstYI RGATCY 2 cut(s) 569, 917
BsuRI GGCC 6 cut(s) 23, 374, 412, 537, 610, 881
BtsCI GGATG 2 cut(s) 218, 480
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 4 cut(s) 340, 457, 588, 834
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 410
CviAII CATG 3 cut(s) 19, 357, 883
DdeI CTNAG 1 cut(s) 688
DpnI GATC 5 cut(s) 223, 571, 717, 887, 919
DpnII GATC 5 cut(s) 221, 569, 715, 885, 917
EaeI YGGCCR 2 cut(s) 21, 372
Eam1104I CTCTTC 5 cut(s) 16, 116, 180, 433, 806
EarI CTCTTC 5 cut(s) 16, 116, 180, 433, 806
Eco130I CCWWGG 2 cut(s) 33, 645
Eco147I AGGCCT 1 cut(s) 610
Eco31I GGTCTC 2 cut(s) 620, 782
Eco57I CTGAAG 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 343
EcoT14I CCWWGG 2 cut(s) 33, 645
ErhI CCWWGG 2 cut(s) 33, 645
Esp3I CGTCTC 2 cut(s) 188, 520
FaeI CATG 3 cut(s) 22, 360, 886
FalI AAGNNNNNCTT 2 cut(s) 715, 747
FaqI GGGAC 2 cut(s) 434, 911
FatI CATG 3 cut(s) 18, 356, 882
FauI CCCGC 1 cut(s) 285
FbaI TGATCA 1 cut(s) 885
Fnu4HI GCNGC 4 cut(s) 381, 465, 468, 797
FokI GGATG 2 cut(s) 225, 467
Fsp4HI GCNGC 4 cut(s) 381, 465, 468, 797
FspBI CTAG 1 cut(s) 93
GlaI GCGC 1 cut(s) 291
GluI GCNGC 4 cut(s) 381, 465, 468, 797
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 6 cut(s) 23, 374, 412, 537, 610, 881
HapII CCGG 1 cut(s) 567
HhaI GCGC 1 cut(s) 292
Hin1II CATG 3 cut(s) 22, 360, 886
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 3 cut(s) 157, 389, 821
HpaII CCGG 1 cut(s) 567
Hpy166II GTNNAC 2 cut(s) 310, 597
Hpy188I TCNGA 3 cut(s) 720, 790, 868
Hpy188III TCNNGA 6 cut(s) 194, 567, 635, 690, 766, 889
Hpy8I GTNNAC 2 cut(s) 310, 597
HpyAV CCTTC 2 cut(s) 868, 871
HpyCH4III ACNGT 6 cut(s) 121, 133, 200, 400, 641, 671
HpyCH4IV ACGT 2 cut(s) 303, 423
HpyCH4V TGCA 4 cut(s) 136, 269, 455, 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 461
HpyF3I CTNAG 1 cut(s) 688
HpySE526I ACGT 2 cut(s) 303, 423
Hsp92II CATG 3 cut(s) 22, 360, 886
HspAI GCGC 1 cut(s) 290
Kpn2I TCCGGA 1 cut(s) 566
Ksp22I TGATCA 1 cut(s) 885
Kzo9I GATC 5 cut(s) 221, 569, 715, 885, 917
LguI GCTCTTC 2 cut(s) 116, 806
LmnI GCTCC 3 cut(s) 101, 234, 712
Lsp1109I GCAGC 4 cut(s) 367, 451, 454, 808
LweI GCATC 1 cut(s) 489
MaeI CTAG 1 cut(s) 93
MaeII ACGT 2 cut(s) 303, 423
MaeIII GTNAC 2 cut(s) 215, 394
MalI GATC 5 cut(s) 223, 571, 717, 887, 919
MboI GATC 5 cut(s) 221, 569, 715, 885, 917
MboII GAAGA 8 cut(s) 3, 103, 167, 170, 447, 450, 793, 949
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 2 cut(s) 569, 917
MhlI GDGCHC 2 cut(s) 344, 599
MlsI TGGCCA 2 cut(s) 23, 374
MluCI AATT 5 cut(s) 83, 283, 579, 700, 940
MluI ACGCGT 1 cut(s) 336
MluNI TGGCCA 2 cut(s) 23, 374
MlyI GAGTC 1 cut(s) 151
MmeI TCCRAC 2 cut(s) 515, 690
MnlI CCTC 8 cut(s) 19, 181, 401, 498, 621, 691, 791, 918
Mox20I TGGCCA 2 cut(s) 23, 374
MroI TCCGGA 1 cut(s) 566
MscI TGGCCA 2 cut(s) 23, 374
MseI TTAA 3 cut(s) 207, 300, 426
Msp20I TGGCCA 2 cut(s) 23, 374
MspI CCGG 1 cut(s) 567
MspR9I CCNGG 1 cut(s) 345
MunI CAATTG 1 cut(s) 579
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 2 cut(s) 292, 338
MwoI GCNNNNNNNGC 2 cut(s) 380, 461
NdeII GATC 5 cut(s) 221, 569, 715, 885, 917
NlaIII CATG 3 cut(s) 22, 360, 886
NlaIV GGNNCC 5 cut(s) 169, 571, 708, 814, 919
NmuCI GTSAC 1 cut(s) 215
PceI AGGCCT 1 cut(s) 610
PciSI GCTCTTC 2 cut(s) 116, 806
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 2 cut(s) 389, 821
PkrI GCNGC 4 cut(s) 382, 466, 469, 798
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 5 cut(s) 169, 571, 708, 814, 919
PspPI GGNCC 1 cut(s) 410
PsuI RGATCY 2 cut(s) 569, 917
SapI GCTCTTC 2 cut(s) 116, 806
SaqAI TTAA 3 cut(s) 207, 300, 426
SatI GCNGC 4 cut(s) 381, 465, 468, 797
Sau3AI GATC 5 cut(s) 221, 569, 715, 885, 917
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 1 cut(s) 151
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 2 cut(s) 344, 599
SfaNI GCATC 1 cut(s) 489
SfcI CTRYAG 1 cut(s) 838
Sse9I AATT 5 cut(s) 83, 283, 579, 700, 940
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 3 cut(s) 141, 292, 324
SspMI CTAG 1 cut(s) 93
StuI AGGCCT 1 cut(s) 610
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 2 cut(s) 33, 645
TaaI ACNGT 6 cut(s) 121, 133, 200, 400, 641, 671
TaiI ACGT 2 cut(s) 306, 426
TaqI TCGA 2 cut(s) 160, 245
TasI AATT 5 cut(s) 83, 283, 579, 700, 940
TfiI GAWTC 2 cut(s) 389, 821
Tru1I TTAA 3 cut(s) 207, 300, 426
Tru9I TTAA 3 cut(s) 207, 300, 426
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 4 cut(s) 380, 464, 467, 796
Tsp45I GTSAC 1 cut(s) 215
TspGWI ACGGA 1 cut(s) 234
TspRI CASTG 1 cut(s) 124
VneI GTGCAC 1 cut(s) 595
XapI RAATTY 2 cut(s) 83, 283
XcmI CCANNNNNNNNNTGG 3 cut(s) 31, 550, 889
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.