Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007105

Physical Location & Seq

Genomic Coordinates Reverse (-)

27287662 .. 27291066

Gene Structure 3405 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1168600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 912 bp

ATGGGAACCAACCACAAGAGACTGATCGGAGAGCTACATGAAGGCCAGAAGACGGCGGCGGAGCTTCAGATTCTGCTCCAGAACCCGTACGGAGATCGTGTCTTGGCGGAGGAGCTTTTGATGAAGATCATGACGACATTCGCAGAGACTCTTTCTGTTCTGACTGCTTCTGATGGCCATGAGGATCACCAGTCTGCTGCTTCCGGTGAGGTTCATCAGGTCAAGCCGGAACCTTCCCAAGTGGAGCACTCGCATTGCGGTGACCGGAGCTCTGAAGATTCCGGTGAGAGTCGAAAGTCTCAGGCTTTCAAGGATCGGAGAGGTTGCTACAAGAGGAGAAAGACATGTCAATCATGGACGGTAACCTCGACCAAAATTGAAGATGGACAGGCTTGGAGAAAATATGGCCAAAAGATAATCCTCAATGCTCCATATCCAAGGGCTTACTTCAGATGCACAAGAAAGTTTGACCAAGGTTGCAAGGCAACCAAACAAGTCCAACAAATCCAAGACAACCCACTCACGTACCAAACCACCTACATCGGCGAGCACACGTGCAGAGCCATGATCAAGCCTTCACCAATGATCATCGGCTCTAATCCCAGGGACTCTCAGACTGTTAGTTCAGAATCTGGGACCCCACATCACCAAAACCCTAGTTTCTTTGGCACATCCGCACCGTCAATTCTCATTGCGAAGCAAGAAGATTACAAAGTGGGGACACCCAGTGATGTAACGGACAACAGTATGTGGCATGATTTGAAGGATCACTTGGATTTATCAGAGACTGTCGGGATCTGCGTGTCTTCCAACGAGGATGTGGTTTCAAACATGCACTGGGATGAGGACATGGATTATGTGGTGAAGTCTATTAATTTTGAGGAAGATTTTAATTTTGAGAGTTTCCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

304

Amino Acids

34.23

Weight (kDa)

5.71

Isoelectric Point (pI)

44.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	127 - 186	1.7e-23	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40750 AT3G56400
fragaria_vesca	FvH4_7g26030
malus_domestica	MD01G1168600.v1.1 MD07G1234700.v1.1
prunus_persica	Prupe.2G265000_v2.0.a1
pyrus_communis	pycom01g18160 pycom07g21230
rosa_chinensis	RchiOBHm_Chr1g0372441 RchiOBHm_Chr1g0372521
rosa_laevigata	RLG00000026893 RLG00000026898
rosa_multiflora	Rmu_co8027390.1_g000001 Rmu_co8028224.1_g000001 Rmu_sc0001755.1_g000001
rosa_roxburghii	Rroxscaffold_4G00284920
rosa_rugosa	Rorug01G0371900
rosa_samantha	Rh1AG380400 Rh1AG381100 Rh1BG344000 Rh1BG344500 Rh1CG357400 Rh1DG375200 Rh1DG375700
rosa_wichuraiana	Rw1G033450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	5 cut(s)	56, 59, 107, 258, 675
AclWI	GGATC	4 cut(s)	192, 321, 774, 803
AcoI	YGGCCR	2 cut(s)	175, 406
AcuI	CTGAAG	3 cut(s)	50, 294, 433
AcvI	CACGTG	1 cut(s)	555
AdeI	CACNNNGTG	1 cut(s)	728
AfaI	GTAC	2 cut(s)	89, 527
AfiI	CCNNNNNNNGG	1 cut(s)	52
AflIII	ACRYGT	2 cut(s)	344, 552
AgsI	TTSAA	4 cut(s)	310, 380, 763, 828
AjnI	CCWGG	1 cut(s)	602
AjuI	GAANNNNNNNTTGG	2 cut(s)	755, 787
AluBI	AGCT	4 cut(s)	34, 64, 115, 270
AluI	AGCT	4 cut(s)	34, 64, 115, 270
Alw21I	GWGCWC	3 cut(s)	249, 272, 552
Alw26I	GTCTC	4 cut(s)	13, 140, 303, 779
AlwI	GGATC	4 cut(s)	192, 321, 774, 803
AlwNI	CAGNNNCTG	3 cut(s)	73, 632, 788
AoxI	GGCC	3 cut(s)	43, 175, 406
ApeKI	GCWGC	1 cut(s)	197
AseI	ATTAAT	1 cut(s)	873
AspS9I	GGNCC	1 cut(s)	636
AsuHPI	GGTGA	7 cut(s)	179, 218, 272, 296, 570, 638, 874
AvaII	GGWCC	1 cut(s)	636
BaeI	ACNNNNGTAYC	2 cut(s)	509, 542
BalI	TGGCCA	2 cut(s)	177, 408
BanII	GRGCYC	1 cut(s)	272
BbrPI	CACGTG	1 cut(s)	555
BbsI	GAAGAC	2 cut(s)	56, 798
Bbv12I	GWGCWC	3 cut(s)	249, 272, 552
BbvI	GCAGC	1 cut(s)	184
BccI	CCATC	2 cut(s)	167, 377
BceAI	ACGGC	1 cut(s)	69
BciT130I	CCWGG	1 cut(s)	604
BclI	TGATCA	2 cut(s)	567, 585
BcoDI	GTCTC	4 cut(s)	13, 140, 303, 779
BfaI	CTAG	1 cut(s)	657
BisI	GCNGC	2 cut(s)	57, 198
BlsI	GCNGC	2 cut(s)	58, 199
Bme1390I	CCNGG	1 cut(s)	604
Bme18I	GGWCC	1 cut(s)	636
BmgT120I	GGNCC	1 cut(s)	636
BmiI	GGNNCC	4 cut(s)	7, 231, 637, 638
BmrFI	CCNGG	1 cut(s)	604
BmrI	ACTGGG	2 cut(s)	720, 847
BmsI	GCATC	1 cut(s)	443
BmuI	ACTGGG	2 cut(s)	720, 847
BpiI	GAAGAC	2 cut(s)	56, 798
BpmI	CTGGAG	1 cut(s)	62
BsaAI	YACGTR	2 cut(s)	525, 555
BsaBI	GATNNNNATC	1 cut(s)	125
BsaJI	CCNNGG	4 cut(s)	437, 472, 602, 603
BsaWI	WCCGGW	3 cut(s)	203, 264, 281
BsaXI	ACNNNNNCTCC	2 cut(s)	84, 114
Bsc4I	CCNNNNNNNGG	1 cut(s)	52
Bse1I	ACTGG	3 cut(s)	190, 726, 842
Bse3DI	GCAATG	2 cut(s)	253, 690
Bse8I	GATNNNNATC	1 cut(s)	125
BseBI	CCWGG	1 cut(s)	604
BseDI	CCNNGG	4 cut(s)	437, 472, 602, 603
BseGI	GGATG	3 cut(s)	671, 823, 847
BseJI	GATNNNNATC	1 cut(s)	125
BseLI	CCNNNNNNNGG	1 cut(s)	52
BseMI	GCAATG	2 cut(s)	253, 690
BseMII	CTCAG	2 cut(s)	314, 626
BseNI	ACTGG	3 cut(s)	190, 726, 842
BseRI	GAGGAG	2 cut(s)	125, 349
BseXI	GCAGC	1 cut(s)	184
BsgI	GTGCAG	1 cut(s)	577
BshFI	GGCC	3 cut(s)	45, 177, 408
BsiHKAI	GWGCWC	3 cut(s)	249, 272, 552
BsiSI	CCGG	4 cut(s)	204, 227, 265, 282
BsiWI	CGTACG	1 cut(s)	87
BslFI	GGGAC	3 cut(s)	620, 649, 733
BslI	CCNNNNNNNGG	1 cut(s)	52
BsmAI	GTCTC	4 cut(s)	13, 140, 303, 779
BsmFI	GGGAC	3 cut(s)	620, 649, 733
BsnI	GGCC	3 cut(s)	45, 177, 408
Bsp1286I	GDGCHC	3 cut(s)	249, 272, 552
Bsp143I	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
BspACI	CCGC	5 cut(s)	56, 59, 107, 258, 675
BspANI	GGCC	3 cut(s)	45, 177, 408
BspCNI	CTCAG	2 cut(s)	313, 625
BspHI	TCATGA	1 cut(s)	129
BspLI	GGNNCC	4 cut(s)	7, 231, 637, 638
BspPI	GGATC	4 cut(s)	192, 321, 774, 803
BsrDI	GCAATG	2 cut(s)	253, 690
BsrI	ACTGG	3 cut(s)	190, 726, 842
BssECI	CCNNGG	4 cut(s)	437, 472, 602, 603
BssMI	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
BssT1I	CCWWGG	2 cut(s)	437, 472
Bst2UI	CCWGG	1 cut(s)	604
Bst4CI	ACNGT	5 cut(s)	361, 619, 681, 746, 790
BstBAI	YACGTR	2 cut(s)	525, 555
BstC8I	GCNNGC	1 cut(s)	548
BstDEI	CTNAG	2 cut(s)	300, 612
BstEII	GGTNACC	2 cut(s)	260, 361
BstF5I	GGATG	3 cut(s)	671, 823, 847
BstKTI	GATC	9 cut(s)	27, 97, 129, 187, 316, 570, 588, 769, 798
BstMAI	GTCTC	4 cut(s)	13, 140, 303, 779
BstMBI	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
BstNI	CCWGG	1 cut(s)	604
BstNSI	RCATGY	2 cut(s)	348, 835
BstPI	GGTNACC	2 cut(s)	260, 361
BstSCI	CCNGG	1 cut(s)	602
BstV1I	GCAGC	1 cut(s)	184
BstV2I	GAAGAC	2 cut(s)	56, 798
BstX2I	RGATCY	1 cut(s)	795
BstYI	RGATCY	1 cut(s)	795
BsuRI	GGCC	3 cut(s)	45, 177, 408
BtsCI	GGATG	3 cut(s)	671, 823, 847
BtsIMutI	CAGTG	2 cut(s)	733, 835
Cac8I	GCNNGC	1 cut(s)	548
CaiI	CAGNNNCTG	3 cut(s)	73, 632, 788
CciI	TCATGA	1 cut(s)	129
Cfr13I	GGNCC	1 cut(s)	636
Csp6I	GTAC	2 cut(s)	88, 526
CviAII	CATG	9 cut(s)	38, 130, 179, 345, 354, 565, 755, 832, 850
CviQI	GTAC	2 cut(s)	88, 526
DdeI	CTNAG	2 cut(s)	300, 612
DpnI	GATC	9 cut(s)	26, 96, 128, 186, 315, 569, 587, 768, 797
DpnII	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
DraIII	CACNNNGTG	1 cut(s)	728
EaeI	YGGCCR	2 cut(s)	175, 406
EciI	GGCGGA	2 cut(s)	74, 122
Ecl136II	GAGCTC	1 cut(s)	270
Eco130I	CCWWGG	2 cut(s)	437, 472
Eco24I	GRGCYC	1 cut(s)	272
Eco47I	GGWCC	1 cut(s)	636
Eco53kI	GAGCTC	1 cut(s)	270
Eco57I	CTGAAG	3 cut(s)	50, 294, 433
Eco72I	CACGTG	1 cut(s)	555
Eco91I	GGTNACC	2 cut(s)	260, 361
EcoICRI	GAGCTC	1 cut(s)	270
EcoO109I	RGGNCCY	1 cut(s)	636
EcoO65I	GGTNACC	2 cut(s)	260, 361
EcoRII	CCWGG	1 cut(s)	602
EcoT14I	CCWWGG	2 cut(s)	437, 472
EcoT38I	GRGCYC	1 cut(s)	272
ErhI	CCWWGG	2 cut(s)	437, 472
FaeI	CATG	9 cut(s)	41, 133, 182, 348, 357, 568, 758, 835, 853
FalI	AAGNNNNNCTT	2 cut(s)	755, 787
FaqI	GGGAC	3 cut(s)	620, 649, 733
FatI	CATG	9 cut(s)	37, 129, 178, 344, 353, 564, 754, 831, 849
FbaI	TGATCA	2 cut(s)	567, 585
Fnu4HI	GCNGC	2 cut(s)	57, 198
FokI	GGATG	3 cut(s)	658, 830, 854
FriOI	GRGCYC	1 cut(s)	272
Fsp4HI	GCNGC	2 cut(s)	57, 198
FspBI	CTAG	1 cut(s)	657
GluI	GCNGC	2 cut(s)	57, 198
GsuI	CTGGAG	1 cut(s)	62
HaeIII	GGCC	3 cut(s)	45, 177, 408
HapII	CCGG	4 cut(s)	204, 227, 265, 282
Hin1II	CATG	9 cut(s)	41, 133, 182, 348, 357, 568, 758, 835, 853
HinfI	GANTC	6 cut(s)	70, 148, 278, 289, 608, 629
HpaII	CCGG	4 cut(s)	204, 227, 265, 282
HphI	GGTGA	7 cut(s)	179, 218, 272, 296, 570, 638, 874
Hpy188III	TCNNGA	3 cut(s)	79, 130, 793
HpyAV	CCTTC	4 cut(s)	35, 243, 585, 757
HpyCH4III	ACNGT	5 cut(s)	361, 619, 681, 746, 790
HpyCH4IV	ACGT	2 cut(s)	524, 554
HpyCH4V	TGCA	4 cut(s)	456, 480, 558, 835
HpyF3I	CTNAG	2 cut(s)	300, 612
HpySE526I	ACGT	2 cut(s)	524, 554
Hsp92II	CATG	9 cut(s)	41, 133, 182, 348, 357, 568, 758, 835, 853
KflI	GGGWCCC	1 cut(s)	636
Ksp22I	TGATCA	2 cut(s)	567, 585
Kzo9I	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
LmnI	GCTCC	6 cut(s)	61, 81, 112, 244, 267, 433
Lsp1109I	GCAGC	1 cut(s)	184
LweI	GCATC	1 cut(s)	443
MaeI	CTAG	1 cut(s)	657
MaeII	ACGT	2 cut(s)	524, 554
MaeIII	GTNAC	3 cut(s)	260, 361, 733
MalI	GATC	9 cut(s)	26, 96, 128, 186, 315, 569, 587, 768, 797
MboI	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
MboII	GAAGA	7 cut(s)	61, 136, 287, 392, 716, 798, 896
MflI	RGATCY	1 cut(s)	795
MhlI	GDGCHC	3 cut(s)	249, 272, 552
MlsI	TGGCCA	2 cut(s)	177, 408
MluCI	AATT	4 cut(s)	375, 684, 874, 892
MluNI	TGGCCA	2 cut(s)	177, 408
MlyI	GAGTC	3 cut(s)	142, 298, 602
MmeI	TCCRAC	2 cut(s)	523, 834
Mox20I	TGGCCA	2 cut(s)	177, 408
MscI	TGGCCA	2 cut(s)	177, 408
MseI	TTAA	2 cut(s)	873, 891
MslI	CAYNNNNRTG	2 cut(s)	258, 840
Msp20I	TGGCCA	2 cut(s)	177, 408
MspI	CCGG	4 cut(s)	204, 227, 265, 282
MspR9I	CCNGG	1 cut(s)	604
MvaI	CCWGG	1 cut(s)	604
NdeII	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
NlaIII	CATG	9 cut(s)	41, 133, 182, 348, 357, 568, 758, 835, 853
NlaIV	GGNNCC	4 cut(s)	7, 231, 637, 638
NmuCI	GTSAC	1 cut(s)	260
NspI	RCATGY	2 cut(s)	348, 835
PagI	TCATGA	1 cut(s)	129
PasI	CCCWGGG	1 cut(s)	603
PciI	ACATGT	1 cut(s)	344
PcsI	WCGNNNNNNNCGW	2 cut(s)	365, 798
PfeI	GAWTC	3 cut(s)	70, 278, 629
Pfl23II	CGTACG	1 cut(s)	87
PkrI	GCNGC	2 cut(s)	58, 199
PleI	GAGTC	3 cut(s)	142, 297, 602
PmaCI	CACGTG	1 cut(s)	555
PmlI	CACGTG	1 cut(s)	555
PpsI	GAGTC	3 cut(s)	142, 297, 602
Ppu21I	YACGTR	2 cut(s)	525, 555
PpuMI	RGGWCCY	1 cut(s)	636
PscI	ACATGT	1 cut(s)	344
PshBI	ATTAAT	1 cut(s)	873
Psp124BI	GAGCTC	1 cut(s)	272
Psp5II	RGGWCCY	1 cut(s)	636
Psp6I	CCWGG	1 cut(s)	602
PspCI	CACGTG	1 cut(s)	555
PspEI	GGTNACC	2 cut(s)	260, 361
PspGI	CCWGG	1 cut(s)	602
PspLI	CGTACG	1 cut(s)	87
PspN4I	GGNNCC	4 cut(s)	7, 231, 637, 638
PspPI	GGNCC	1 cut(s)	636
PspPPI	RGGWCCY	1 cut(s)	636
PstNI	CAGNNNCTG	3 cut(s)	73, 632, 788
PsuI	RGATCY	1 cut(s)	795
RsaI	GTAC	2 cut(s)	89, 527
RsaNI	GTAC	2 cut(s)	88, 526
RseI	CAYNNNNRTG	2 cut(s)	258, 840
SacI	GAGCTC	1 cut(s)	272
SaqAI	TTAA	2 cut(s)	873, 891
SatI	GCNGC	2 cut(s)	57, 198
Sau3AI	GATC	9 cut(s)	24, 94, 126, 184, 313, 567, 585, 766, 795
Sau96I	GGNCC	1 cut(s)	636
SchI	GAGTC	3 cut(s)	142, 298, 602
ScrFI	CCNGG	1 cut(s)	604
SduI	GDGCHC	3 cut(s)	249, 272, 552
SfaNI	GCATC	1 cut(s)	443
SinI	GGWCC	1 cut(s)	636
SmiMI	CAYNNNNRTG	2 cut(s)	258, 840
Sse9I	AATT	4 cut(s)	375, 684, 874, 892
SsiI	CCGC	5 cut(s)	56, 59, 107, 258, 675
SspMI	CTAG	1 cut(s)	657
SstI	GAGCTC	1 cut(s)	272
StyD4I	CCNGG	1 cut(s)	602
StyI	CCWWGG	2 cut(s)	437, 472
TaaI	ACNGT	5 cut(s)	361, 619, 681, 746, 790
TaiI	ACGT	2 cut(s)	527, 557
TaqI	TCGA	2 cut(s)	292, 368
TasI	AATT	4 cut(s)	375, 684, 874, 892
TauI	GCSGC	1 cut(s)	59
TfiI	GAWTC	3 cut(s)	70, 278, 629
Tru1I	TTAA	2 cut(s)	873, 891
Tru9I	TTAA	2 cut(s)	873, 891
TscAI	CASTG	2 cut(s)	733, 842
TseFI	GTSAC	1 cut(s)	260
TseI	GCWGC	1 cut(s)	197
Tsp45I	GTSAC	1 cut(s)	260
TspDTI	ATGAA	3 cut(s)	54, 137, 203
TspGWI	ACGGA	2 cut(s)	105, 752
TspRI	CASTG	2 cut(s)	733, 842
VpaK11BI	GGWCC	1 cut(s)	636
VspI	ATTAAT	1 cut(s)	873
XceI	RCATGY	2 cut(s)	348, 835
XcmI	CCANNNNNNNNNTGG	1 cut(s)	817
XspI	CTAG	1 cut(s)	657

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.