Rh1AG381100
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0007105
Physical Location & Seq
Forward (+)
61272671 .. 61295024
22354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG381100.1

Sequence Viewer

Length: 852 bp
ATGGAAGAAGTTCATATGTGGAAGTCTGCTCTAACTGAAGCCACCAACTTATCTGGGTGGGATTCTCGTAATTACAAAATCCTCAAGTCGGATCATATGTTCATGGCATATGTGCCATGGTCTGAGTATCGCTTCATTGAAGAGGGAAAGCTGGTCAATGAACGGTATTTCACTGAGGCCACATTTGAGATTGTAGTAGAAAATAGAGTTGATAGACATGGTTTCGGGACAGTAATAAAACGCCATTGCATCACAAGTTGCGGAGTTCGTTTCTTTCATGGTGATTACATGGAGGAGCAAATTCTAACCATCAGCGAAACCAAATCTCATAATGACAGCTGTGACATCCTTTCGGTTAGTAGGAAAGGAAAAGAGGGGGTTGGGGTGCGGTTGGAGGAGTCTCCAGCGGAAGGCAGCTCTGCCGGTGAGAGCAAGAAGAGGCCAGGTTCAGAGGATCAGAAGGGTTCCAACAAGCGAAGGGCTTACTTCAGATGTACCCGCAAGTATGATCAAGGTTGCCAAGCAACCAAACGAGTTCAACAAGTCCAAGACACCCCAAAGGAGTACGAAATTACCTACAAAGGAAACCACACGTGCGTAAACATGATGATGATCCTGGGAGCCTCCGATCCTGCTACTTGGCCGACCCCAGTTTGCAGTAAGAAAGAACATGGCGATCTCTCTAGCTCACTGTCCCTGATTCCTTTCGTTCCGGAGAAGAAAGAAGAGTGCAAGGAGGGGACGACGGCGAGTGGTCTGGTGGGCAACGTTAACGATACTAGTGATATGTGGACGGATTTTGAGTCGCCGGAGTTTGTGTTCCCGGATTTAGGGACTGAACAGTCACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

283

Amino Acids

32.08

Weight (kDa)

5.59

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 149 - 199 1.6e-12 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 841
AccIII TCCGGA 1 cut(s) 712
AciI CCGC 4 cut(s) 261, 388, 407, 499
AclI AACGTT 1 cut(s) 768
AclWI GGATC 4 cut(s) 99, 462, 607, 623
AcoI YGGCCR 1 cut(s) 641
AcsI RAATTY 1 cut(s) 300
AcuI CTGAAG 2 cut(s) 57, 472
AcvI CACGTG 1 cut(s) 594
AfaI GTAC 2 cut(s) 496, 566
AfiI CCNNNNNNNGG 3 cut(s) 88, 410, 830
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 2 cut(s) 140, 539
AhlI ACTAGT 1 cut(s) 779
AjnI CCWGG 2 cut(s) 442, 615
AloI GAACNNNNNNTCC 4 cut(s) 83, 115, 803, 835
AluBI AGCT 4 cut(s) 151, 339, 417, 687
AluI AGCT 4 cut(s) 151, 339, 417, 687
Alw26I GTCTC 1 cut(s) 405
AlwI GGATC 4 cut(s) 99, 462, 607, 623
Aor13HI TCCGGA 1 cut(s) 712
AoxI GGCC 3 cut(s) 177, 440, 641
ApeKI GCWGC 1 cut(s) 414
ApoI RAATTY 1 cut(s) 300
Asp700I GAANNNNTTC 1 cut(s) 9
AsuC2I CCSGG 1 cut(s) 824
AsuHPI GGTGA 3 cut(s) 293, 437, 837
BbrPI CACGTG 1 cut(s) 594
BbvI GCAGC 1 cut(s) 426
BccI CCATC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 762
BciT130I CCWGG 2 cut(s) 444, 617
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 824
BcoDI GTCTC 1 cut(s) 405
BcuI ACTAGT 1 cut(s) 779
BfaI CTAG 2 cut(s) 684, 780
BisI GCNGC 1 cut(s) 415
BlsI GCNGC 1 cut(s) 416
Bme1390I CCNGG 3 cut(s) 444, 617, 824
BmiI GGNNCC 2 cut(s) 466, 622
BmrFI CCNGG 3 cut(s) 444, 617, 824
BmrI ACTGGG 1 cut(s) 644
BmsI GCATC 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 644
BpmI CTGGAG 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 68
BpuMI CCSGG 1 cut(s) 824
BsaAI YACGTR 1 cut(s) 594
BsaBI GATNNNNATC 1 cut(s) 611
BsaJI CCNNGG 2 cut(s) 116, 616
BsaWI WCCGGW 1 cut(s) 712
BsaXI ACNNNNNCTCC 2 cut(s) 803, 833
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 410, 830
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 1 cut(s) 650
Bse3DI GCAATG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 611
BseAI TCCGGA 1 cut(s) 712
BseBI CCWGG 2 cut(s) 444, 617
BseDI CCNNGG 2 cut(s) 116, 616
BseGI GGATG 1 cut(s) 345
BseJI GATNNNNATC 1 cut(s) 611
BseLI CCNNNNNNNGG 3 cut(s) 88, 410, 830
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 2 cut(s) 114, 165
BseNI ACTGG 1 cut(s) 650
BseRI GAGGAG 2 cut(s) 308, 410
BseXI GCAGC 1 cut(s) 426
BshFI GGCC 3 cut(s) 179, 442, 643
BsiSI CCGG 4 cut(s) 423, 713, 809, 824
BslFI GGGAC 4 cut(s) 241, 679, 754, 847
BslI CCNNNNNNNGG 3 cut(s) 88, 410, 830
BsmAI GTCTC 1 cut(s) 405
BsmFI GGGAC 4 cut(s) 241, 679, 754, 847
BsnI GGCC 3 cut(s) 179, 442, 643
Bsp13I TCCGGA 1 cut(s) 712
Bsp143I GATC 6 cut(s) 91, 454, 508, 612, 628, 676
Bsp19I CCATGG 1 cut(s) 116
BspACI CCGC 4 cut(s) 261, 388, 407, 499
BspANI GGCC 3 cut(s) 179, 442, 643
BspCNI CTCAG 2 cut(s) 115, 166
BspEI TCCGGA 1 cut(s) 712
BspLI GGNNCC 2 cut(s) 466, 622
BspPI GGATC 4 cut(s) 99, 462, 607, 623
BsrDI GCAATG 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 1 cut(s) 650
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 2 cut(s) 116, 616
BssMI GATC 6 cut(s) 91, 454, 508, 612, 628, 676
BssT1I CCWWGG 1 cut(s) 116
Bst2UI CCWGG 2 cut(s) 444, 617
Bst4CI ACNGT 4 cut(s) 165, 232, 693, 843
Bst6I CTCTTC 3 cut(s) 135, 431, 720
BstBAI YACGTR 1 cut(s) 594
BstDEI CTNAG 2 cut(s) 123, 174
BstDSI CCRYGG 1 cut(s) 116
BstF5I GGATG 1 cut(s) 345
BstKTI GATC 6 cut(s) 94, 457, 511, 615, 631, 679
BstMAI GTCTC 1 cut(s) 405
BstMBI GATC 6 cut(s) 91, 454, 508, 612, 628, 676
BstNI CCWGG 2 cut(s) 444, 617
BstSCI CCNGG 3 cut(s) 442, 615, 822
BstV1I GCAGC 1 cut(s) 426
BsuRI GGCC 3 cut(s) 179, 442, 643
BtgI CCRYGG 1 cut(s) 116
BtsCI GGATG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 171, 689
Cfr10I RCCGGY 1 cut(s) 422
Csp6I GTAC 2 cut(s) 495, 565
CviAII CATG 7 cut(s) 103, 117, 218, 278, 289, 604, 671
CviQI GTAC 2 cut(s) 495, 565
DdeI CTNAG 2 cut(s) 123, 174
DpnI GATC 6 cut(s) 93, 456, 510, 614, 630, 678
DpnII GATC 6 cut(s) 91, 454, 508, 612, 628, 676
DrdI GACNNNNNNGTC 1 cut(s) 841
DseDI GACNNNNNNGTC 1 cut(s) 841
EaeI YGGCCR 1 cut(s) 641
Eam1104I CTCTTC 3 cut(s) 135, 431, 720
EarI CTCTTC 3 cut(s) 135, 431, 720
Eco130I CCWWGG 1 cut(s) 116
Eco57I CTGAAG 2 cut(s) 57, 472
Eco72I CACGTG 1 cut(s) 594
EcoRII CCWGG 2 cut(s) 442, 615
EcoT14I CCWWGG 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 116
FaeI CATG 7 cut(s) 106, 120, 221, 281, 292, 607, 674
FaqI GGGAC 4 cut(s) 241, 679, 754, 847
FatI CATG 7 cut(s) 102, 116, 217, 277, 288, 603, 670
FauI CCCGC 1 cut(s) 506
FauNDI CATATG 3 cut(s) 15, 96, 109
FbaI TGATCA 1 cut(s) 508
Fnu4HI GCNGC 1 cut(s) 415
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 1 cut(s) 415
FspBI CTAG 2 cut(s) 684, 780
GluI GCNGC 1 cut(s) 415
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 3 cut(s) 179, 442, 643
HapII CCGG 4 cut(s) 423, 713, 809, 824
Hin1II CATG 7 cut(s) 106, 120, 221, 281, 292, 607, 674
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 4 cut(s) 62, 398, 700, 803
HpaI GTTAAC 1 cut(s) 772
HpaII CCGG 4 cut(s) 423, 713, 809, 824
HphI GGTGA 3 cut(s) 293, 437, 837
Hpy166II GTNNAC 3 cut(s) 601, 772, 792
Hpy188I TCNGA 6 cut(s) 91, 124, 451, 459, 491, 628
Hpy188III TCNNGA 2 cut(s) 226, 713
Hpy8I GTNNAC 3 cut(s) 601, 772, 792
Hpy99I CGWCG 1 cut(s) 748
HpyAV CCTTC 3 cut(s) 404, 454, 471
HpyCH4III ACNGT 4 cut(s) 165, 232, 693, 843
HpyCH4IV ACGT 2 cut(s) 593, 768
HpyCH4V TGCA 3 cut(s) 249, 657, 732
HpyF3I CTNAG 2 cut(s) 123, 174
HpySE526I ACGT 2 cut(s) 593, 768
Hsp92II CATG 7 cut(s) 106, 120, 221, 281, 292, 607, 674
Kpn2I TCCGGA 1 cut(s) 712
Ksp22I TGATCA 1 cut(s) 508
KspAI GTTAAC 1 cut(s) 772
Kzo9I GATC 6 cut(s) 91, 454, 508, 612, 628, 676
LmnI GCTCC 2 cut(s) 295, 620
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 1 cut(s) 258
MaeI CTAG 2 cut(s) 684, 780
MaeII ACGT 2 cut(s) 593, 768
MaeIII GTNAC 2 cut(s) 341, 843
MalI GATC 6 cut(s) 93, 456, 510, 614, 630, 678
MboI GATC 6 cut(s) 91, 454, 508, 612, 628, 676
MboII GAAGA 5 cut(s) 17, 152, 448, 730, 737
MluCI AATT 3 cut(s) 70, 300, 570
MlyI GAGTC 2 cut(s) 407, 812
MmeI TCCRAC 3 cut(s) 69, 372, 492
MroI TCCGGA 1 cut(s) 712
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 1 cut(s) 771
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 2 cut(s) 339, 407
MspI CCGG 4 cut(s) 423, 713, 809, 824
MspR9I CCNGG 3 cut(s) 444, 617, 824
MvaI CCWGG 2 cut(s) 444, 617
NciI CCSGG 1 cut(s) 824
NcoI CCATGG 1 cut(s) 116
NdeI CATATG 3 cut(s) 15, 96, 109
NdeII GATC 6 cut(s) 91, 454, 508, 612, 628, 676
NlaIII CATG 7 cut(s) 106, 120, 221, 281, 292, 607, 674
NlaIV GGNNCC 2 cut(s) 466, 622
NmuCI GTSAC 2 cut(s) 341, 843
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 2 cut(s) 62, 700
PfoI TCCNGGA 1 cut(s) 822
PkrI GCNGC 1 cut(s) 416
PleI GAGTC 2 cut(s) 406, 811
PmaCI CACGTG 1 cut(s) 594
PmlI CACGTG 1 cut(s) 594
PpsI GAGTC 2 cut(s) 406, 811
Ppu21I YACGTR 1 cut(s) 594
Psp1406I AACGTT 1 cut(s) 768
Psp6I CCWGG 2 cut(s) 442, 615
PspCI CACGTG 1 cut(s) 594
PspGI CCWGG 2 cut(s) 442, 615
PspN4I GGNNCC 2 cut(s) 466, 622
PvuII CAGCTG 1 cut(s) 339
RsaI GTAC 2 cut(s) 496, 566
RsaNI GTAC 2 cut(s) 495, 565
RseI CAYNNNNRTG 1 cut(s) 608
SaqAI TTAA 1 cut(s) 771
SatI GCNGC 1 cut(s) 415
Sau3AI GATC 6 cut(s) 91, 454, 508, 612, 628, 676
SchI GAGTC 2 cut(s) 407, 812
ScrFI CCNGG 3 cut(s) 444, 617, 824
SetI ASST 9 cut(s) 153, 341, 419, 448, 517, 578, 596, 689, 771
SfaNI GCATC 1 cut(s) 258
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
SpeI ACTAGT 1 cut(s) 779
Sse9I AATT 3 cut(s) 70, 300, 570
SsiI CCGC 4 cut(s) 261, 388, 407, 499
SspMI CTAG 2 cut(s) 684, 780
StyD4I CCNGG 3 cut(s) 442, 615, 822
StyI CCWWGG 1 cut(s) 116
TaaI ACNGT 4 cut(s) 165, 232, 693, 843
TaiI ACGT 2 cut(s) 596, 771
TasI AATT 3 cut(s) 70, 300, 570
TfiI GAWTC 2 cut(s) 62, 700
Tru1I TTAA 1 cut(s) 771
Tru9I TTAA 1 cut(s) 771
TscAI CASTG 2 cut(s) 178, 696
TseFI GTSAC 2 cut(s) 341, 843
TseI GCWGC 1 cut(s) 414
Tsp45I GTSAC 2 cut(s) 341, 843
TspDTI ATGAA 4 cut(s) 91, 124, 174, 266
TspGWI ACGGA 1 cut(s) 809
TspRI CASTG 2 cut(s) 178, 696
XapI RAATTY 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 2 cut(s) 684, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.