MD01G1182500.v1.1

Cell number regulator 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007068
Physical Location & Seq
Reverse (-)
28117262 .. 28119753
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1182500.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGCGGATGGGACTGCGCACTCGCGGTATGTGAAGCTGACGAAAGAACAAGCGCCGGCGGAGGACATTAATCCCGGCGAGCTCAATCAGCCCATCGAAGTTCCTCAGGTTTATGGGTTGAATGTTCACAGGTGTAACGAATGTGGACAGCCTTTACCTGACAGCTATCAGGCCCCTGCAGATGAGCCTTGGACAACCGGAATTTTTGGCTGTGCTGAAGATAGAGAAAGTTGCTTGACAGGGTTATTTTGTCCATGTGTTCTGTTTGGGCGCAATGTTGAGAGCCTAAGAGACGATACCCCTTGGAACAGACCGTGCATTTGTCATGCCGTTTGTATTGAAGGTGGTATGACTCTGGCAGCATTAACTGCAATCTTCCATGGCATTGACCCAAGGACTTCATTTCTTATTTGCGAGGGTCTGTTATTTTCTTGGTGGATGTGTGGGATATATACAGGTCTTGTCCGGCAATCCTTGCAGAAGAAGTATCATCTAAAGAACTCGCCGTGTGACCCATGCTTGACACACTGCTGCTTGCACTGGTGCGCCTTGTGCCAAGAGCACAGGGAGATGAAGGGACGCCTCTCGGACAATGCTGTGATGCCAATGACTGTCGTCAACCCGCCCCAAGTTCAACAGATGAAGTCTGCTGACGACAACCAGGATTCTGCTGCACCATCCTCTACCGACAATACCAACAATAATGGCCACACCAATATGGAGATGCAGGCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

246

Amino Acids

27.17

Weight (kDa)

5.17

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 64 - 190 3e-27 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 18
AccII CGCG 1 cut(s) 25
AciI CCGC 4 cut(s) 5, 25, 59, 623
AcoI YGGCCR 1 cut(s) 706
AcsI RAATTY 1 cut(s) 201
AcuI CTGAAG 1 cut(s) 237
AcyI GRCGYC 1 cut(s) 580
AgsI TTSAA 3 cut(s) 121, 341, 635
AjnI CCWGG 1 cut(s) 660
AluBI AGCT 3 cut(s) 37, 82, 165
AluI AGCT 3 cut(s) 37, 82, 165
Alw21I GWGCWC 2 cut(s) 84, 564
Alw26I GTCTC 1 cut(s) 285
AoxI GGCC 2 cut(s) 171, 706
ApeKI GCWGC 3 cut(s) 359, 531, 671
ApoI RAATTY 1 cut(s) 201
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AseI ATTAAT 1 cut(s) 69
AspLEI GCGC 4 cut(s) 19, 55, 273, 548
AspS9I GGNCC 1 cut(s) 172
AsuC2I CCSGG 1 cut(s) 75
AxyI CCTNAGG 1 cut(s) 105
BalI TGGCCA 1 cut(s) 708
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 84, 564
BbvI GCAGC 3 cut(s) 371, 518, 658
BccI CCATC 3 cut(s) 2, 101, 685
BceAI ACGGC 2 cut(s) 314, 490
BciT130I CCWGG 1 cut(s) 662
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 285
BfmI CTRYAG 1 cut(s) 177
BfoI RGCGCY 1 cut(s) 56
BisI GCNGC 3 cut(s) 360, 532, 672
BlsI GCNGC 3 cut(s) 361, 533, 673
Bme1390I CCNGG 2 cut(s) 75, 662
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 2 cut(s) 75, 662
BmsI GCATC 2 cut(s) 591, 714
BoxI GACNNNNGTC 1 cut(s) 614
BpuMI CCSGG 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 580
BsaJI CCNNGG 4 cut(s) 188, 302, 379, 392
BsaWI WCCGGW 1 cut(s) 197
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 545
Bse21I CCTNAGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 1 cut(s) 662
BseDI CCNNGG 4 cut(s) 188, 302, 379, 392
BseGI GGATG 3 cut(s) 13, 444, 677
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 1 cut(s) 545
BseXI GCAGC 3 cut(s) 371, 518, 658
BsgI GTGCAG 1 cut(s) 657
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 2 cut(s) 173, 708
BsiHKAI GWGCWC 2 cut(s) 84, 564
BsiSI CCGG 4 cut(s) 56, 75, 198, 466
BslFI GGGAC 2 cut(s) 25, 591
BsmAI GTCTC 1 cut(s) 285
BsmBI CGTCTC 1 cut(s) 285
BsmFI GGGAC 2 cut(s) 25, 591
BsnI GGCC 2 cut(s) 173, 708
Bsp1286I GDGCHC 2 cut(s) 84, 564
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 4 cut(s) 5, 25, 59, 623
BspANI GGCC 2 cut(s) 173, 708
BspCNI CTCAG 1 cut(s) 118
BspFNI CGCG 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 545
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 4 cut(s) 188, 302, 379, 392
BssNI GRCGYC 1 cut(s) 580
BssT1I CCWWGG 4 cut(s) 188, 302, 379, 392
Bst2UI CCWGG 1 cut(s) 662
Bst4CI ACNGT 2 cut(s) 315, 613
BstACI GRCGYC 1 cut(s) 580
BstAPI GCANNNNNTGC 2 cut(s) 368, 475
BstC8I GCNNGC 4 cut(s) 57, 80, 536, 729
BstDEI CTNAG 2 cut(s) 105, 287
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 3 cut(s) 13, 444, 677
BstFNI CGCG 1 cut(s) 25
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 4 cut(s) 19, 55, 273, 548
BstMAI GTCTC 1 cut(s) 285
BstMWI GCNNNNNNNGC 4 cut(s) 88, 368, 475, 552
BstNI CCWGG 1 cut(s) 662
BstPAI GACNNNNGTC 1 cut(s) 614
BstSCI CCNGG 2 cut(s) 73, 660
BstSFI CTRYAG 1 cut(s) 177
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 3 cut(s) 371, 518, 658
Bsu36I CCTNAGG 1 cut(s) 105
BsuRI GGCC 2 cut(s) 173, 708
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 3 cut(s) 13, 444, 677
BtsI GCAGTG 1 cut(s) 526
BtsIMutI CAGTG 2 cut(s) 526, 538
Cac8I GCNNGC 4 cut(s) 57, 80, 536, 729
CfoI GCGC 4 cut(s) 19, 55, 273, 548
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 172
CseI GACGC 1 cut(s) 588
CviAII CATG 4 cut(s) 255, 326, 380, 516
DdeI CTNAG 2 cut(s) 105, 287
EaeI YGGCCR 1 cut(s) 706
EciI GGCGGA 2 cut(s) 20, 74
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 4 cut(s) 188, 302, 379, 392
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 237
Eco81I CCTNAGG 1 cut(s) 105
EcoICRI GAGCTC 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 660
EcoT14I CCWWGG 4 cut(s) 188, 302, 379, 392
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 4 cut(s) 188, 302, 379, 392
Esp3I CGTCTC 1 cut(s) 285
FaeI CATG 4 cut(s) 258, 329, 383, 519
FaqI GGGAC 2 cut(s) 25, 591
FatI CATG 4 cut(s) 254, 325, 379, 515
FauI CCCGC 1 cut(s) 630
Fnu4HI GCNGC 3 cut(s) 360, 532, 672
FokI GGATG 3 cut(s) 20, 451, 664
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 3 cut(s) 360, 532, 672
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 4 cut(s) 18, 54, 272, 547
GluI GCNGC 3 cut(s) 360, 532, 672
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 173, 708
HapII CCGG 4 cut(s) 56, 75, 198, 466
HgaI GACGC 1 cut(s) 588
HhaI GCGC 4 cut(s) 19, 55, 273, 548
Hin1I GRCGYC 1 cut(s) 580
Hin1II CATG 4 cut(s) 258, 329, 383, 519
Hin6I GCGC 4 cut(s) 17, 53, 271, 546
HinP1I GCGC 4 cut(s) 17, 53, 271, 546
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HinfI GANTC 2 cut(s) 352, 665
HpaII CCGG 4 cut(s) 56, 75, 198, 466
Hpy166II GTNNAC 3 cut(s) 127, 146, 619
Hpy188I TCNGA 1 cut(s) 589
Hpy8I GTNNAC 3 cut(s) 127, 146, 619
HpyAV CCTTC 2 cut(s) 335, 568
HpyCH4III ACNGT 2 cut(s) 315, 613
HpyCH4V TGCA 7 cut(s) 179, 318, 371, 478, 538, 674, 727
HpyF10VI GCNNNNNNNGC 4 cut(s) 88, 368, 475, 552
HpyF3I CTNAG 2 cut(s) 105, 287
Hsp92I GRCGYC 1 cut(s) 580
Hsp92II CATG 4 cut(s) 258, 329, 383, 519
HspAI GCGC 4 cut(s) 17, 53, 271, 546
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Lsp1109I GCAGC 3 cut(s) 371, 518, 658
LweI GCATC 2 cut(s) 591, 714
MaeIII GTNAC 2 cut(s) 134, 509
MboII GAAGA 3 cut(s) 230, 367, 493
MhlI GDGCHC 2 cut(s) 84, 564
MlsI TGGCCA 1 cut(s) 708
MluCI AATT 1 cut(s) 201
MluNI TGGCCA 1 cut(s) 708
MlyI GAGTC 1 cut(s) 346
MnlI CCTC 5 cut(s) 55, 114, 409, 593, 691
Mox20I TGGCCA 1 cut(s) 708
MreI CGCCGGCG 1 cut(s) 55
MroNI GCCGGC 1 cut(s) 55
MscI TGGCCA 1 cut(s) 708
MseI TTAA 2 cut(s) 69, 365
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 1 cut(s) 708
MspI CCGG 4 cut(s) 56, 75, 198, 466
MspR9I CCNGG 2 cut(s) 75, 662
MvaI CCWGG 1 cut(s) 662
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 4 cut(s) 88, 368, 475, 552
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 75
NcoI CCATGG 1 cut(s) 379
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 4 cut(s) 258, 329, 383, 519
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 509
NsbI TGCGCA 1 cut(s) 18
PdiI GCCGGC 1 cut(s) 57
PfeI GAWTC 1 cut(s) 665
PkrI GCNGC 3 cut(s) 361, 533, 673
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PshAI GACNNNNGTC 1 cut(s) 614
PshBI ATTAAT 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 660
PspGI CCWGG 1 cut(s) 660
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 1 cut(s) 172
PstI CTGCAG 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 716
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 69, 365
SatI GCNGC 3 cut(s) 360, 532, 672
Sau96I GGNCC 1 cut(s) 172
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 75, 662
SduI GDGCHC 2 cut(s) 84, 564
SetI ASST 8 cut(s) 39, 84, 111, 134, 160, 167, 346, 460
SfaNI GCATC 2 cut(s) 591, 714
SfcI CTRYAG 1 cut(s) 177
SgrAI CRCCGGYG 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 716
Sse9I AATT 1 cut(s) 201
SsiI CCGC 4 cut(s) 5, 25, 59, 623
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 73, 660
StyI CCWWGG 4 cut(s) 188, 302, 379, 392
TaaI ACNGT 2 cut(s) 315, 613
TaqI TCGA 1 cut(s) 96
TasI AATT 1 cut(s) 201
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 2 cut(s) 69, 365
Tru9I TTAA 2 cut(s) 69, 365
TscAI CASTG 2 cut(s) 533, 545
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 3 cut(s) 359, 531, 671
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 3 cut(s) 390, 587, 656
TspRI CASTG 2 cut(s) 533, 545
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.