MD07G1253100.v1.1

Cell number regulator 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007068
Physical Location & Seq
Reverse (-)
32061189 .. 32064338
3150 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1253100.v1.1.491

Sequence Viewer

Length: 732 bp
ATGGCGGACGGAACTGCGCACTCGCGGTATGTGAAGCTGACGAAGGATCAAGCGCCGGCCGAGGACATTAAGCCCGGCGAGCTCAATCAGCCCATCGAAGTTCCCCAGTCGAATGTTCACAGGTGTCACCAATGCGGACAGCCTTTACCCGAGAGCTATCTGCCCCCTAGAGATGAGCCTTGGACAACCGGAATTTTTGGCTGTGCTGAAGATAGAGAAAGTTGCTTGACAGGGTTATTTTGTCCATGTGTTCTGTTTGGGCGCAATGTTATGAGCCAAAGCGTAAGCAATGAGACCCCTTGGCACAGACCATGCATTTCTCATGCTGCTTGTGTTTGTATTGAAGGTGGTTTGGCTCTGGCAGCATTAACTGCAATCTTCCATGGTGTTGACCCATGGATTTCAATTACTATTTGCGAGGGTCTGTTATGTTCTTGGTGGATGCGTGTGGTATTTGCAGGTCTTGTCCGGCAAGCCTTGCAGAAGAAGTATCATCTACAGAACTCACCGTGTCACCCATGCTTGACACACTCCTGCTTGCACTGGTGCGCCTTGTGCCAAGAGCACAGGGAGATGAAGGGACGCCTCTCGGACAATTCTGTGATGCCAATGACCGTCGTCAACCCACCTCAAGTTCAAGAGATGAAGTCTGCTGACGACAACCAGGACTCTGCTTCACCATCCTCTACCAACAATAGTGGCCACACCGATCTCCAGATGCAGGCGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

244

Amino Acids

26.86

Weight (kDa)

5.91

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 61 - 191 7.2e-23 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 18
Acc36I ACCTGC 1 cut(s) 449
AccII CGCG 1 cut(s) 25
AciI CCGC 3 cut(s) 5, 25, 135
AclWI GGATC 1 cut(s) 54
AcoI YGGCCR 2 cut(s) 57, 700
AcsI RAATTY 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 583
AfiI CCNNNNNNNGG 1 cut(s) 721
AgsI TTSAA 3 cut(s) 344, 405, 638
AjnI CCWGG 1 cut(s) 663
AluBI AGCT 3 cut(s) 37, 82, 156
AluI AGCT 3 cut(s) 37, 82, 156
Alw21I GWGCWC 2 cut(s) 84, 567
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 1 cut(s) 54
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 57, 700
ApeKI GCWGC 2 cut(s) 326, 362
ApoI RAATTY 1 cut(s) 192
AspLEI GCGC 4 cut(s) 19, 55, 264, 551
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 4 cut(s) 119, 498, 506, 669
AvaI CYCGRG 1 cut(s) 149
BalI TGGCCA 1 cut(s) 702
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 84, 567
BbvI GCAGC 2 cut(s) 313, 374
BccI CCATC 2 cut(s) 101, 688
BciT130I CCWGG 1 cut(s) 665
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 497
BfoI RGCGCY 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 449
BisI GCNGC 2 cut(s) 327, 363
BlsI GCNGC 2 cut(s) 328, 364
Bme1390I CCNGG 2 cut(s) 75, 665
BmeT110I CYCGRG 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 75, 665
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 3 cut(s) 432, 594, 708
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 617
BpmI CTGGAG 1 cut(s) 698
BpuEI CTTGAG 1 cut(s) 615
BpuMI CCSGG 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 583
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 5 cut(s) 60, 179, 299, 382, 395
BsaWI WCCGGW 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 2 cut(s) 106, 548
Bse3DI GCAATG 2 cut(s) 271, 295
BseBI CCWGG 1 cut(s) 665
BseDI CCNNGG 5 cut(s) 60, 179, 299, 382, 395
BseGI GGATG 2 cut(s) 447, 680
BseLI CCNNNNNNNGG 1 cut(s) 721
BseMI GCAATG 2 cut(s) 271, 295
BseNI ACTGG 2 cut(s) 106, 548
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 313, 374
Bsh1236I CGCG 1 cut(s) 25
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 702
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 84, 567
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 4 cut(s) 56, 75, 189, 469
BslFI GGGAC 1 cut(s) 594
BslI CCNNNNNNNGG 1 cut(s) 721
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 594
BsnI GGCC 2 cut(s) 59, 702
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 84, 567
Bsp143I GATC 2 cut(s) 46, 709
Bsp19I CCATGG 2 cut(s) 382, 395
BspACI CCGC 3 cut(s) 5, 25, 135
BspANI GGCC 2 cut(s) 59, 702
BspFNI CGCG 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 449
BspPI GGATC 1 cut(s) 54
BspTNI GGTCTC 1 cut(s) 287
BsrDI GCAATG 2 cut(s) 271, 295
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 2 cut(s) 106, 548
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 5 cut(s) 60, 179, 299, 382, 395
BssMI GATC 2 cut(s) 46, 709
BssNI GRCGYC 1 cut(s) 583
BssT1I CCWWGG 4 cut(s) 179, 299, 382, 395
Bst2UI CCWGG 1 cut(s) 665
Bst4CI ACNGT 2 cut(s) 510, 616
BstACI GRCGYC 1 cut(s) 583
BstAPI GCANNNNNTGC 2 cut(s) 371, 478
BstC8I GCNNGC 5 cut(s) 57, 80, 474, 539, 723
BstDSI CCRYGG 2 cut(s) 382, 395
BstF5I GGATG 2 cut(s) 447, 680
BstFNI CGCG 1 cut(s) 25
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 4 cut(s) 19, 55, 264, 551
BstKTI GATC 2 cut(s) 49, 712
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 2 cut(s) 46, 709
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 6 cut(s) 79, 88, 362, 371, 478, 555
BstNI CCWGG 1 cut(s) 665
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 2 cut(s) 73, 663
BstSFI CTRYAG 1 cut(s) 497
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 313, 374
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 702
BtgI CCRYGG 2 cut(s) 382, 395
BtsCI GGATG 2 cut(s) 447, 680
BtsIMutI CAGTG 1 cut(s) 541
BveI ACCTGC 1 cut(s) 449
Cac8I GCNNGC 5 cut(s) 57, 80, 474, 539, 723
CfoI GCGC 4 cut(s) 19, 55, 264, 551
Cfr10I RCCGGY 1 cut(s) 55
CseI GACGC 1 cut(s) 591
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CviAII CATG 6 cut(s) 246, 312, 323, 383, 396, 519
DpnI GATC 2 cut(s) 48, 711
DpnII GATC 2 cut(s) 46, 709
EaeI YGGCCR 2 cut(s) 57, 700
EagI CGGCCG 1 cut(s) 57
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 4 cut(s) 179, 299, 382, 395
Eco24I GRGCYC 1 cut(s) 84
Eco31I GGTCTC 1 cut(s) 287
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 663
EcoT14I CCWWGG 4 cut(s) 179, 299, 382, 395
EcoT22I ATGCAT 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 4 cut(s) 179, 299, 382, 395
FaeI CATG 6 cut(s) 249, 315, 326, 386, 399, 522
FaiI YATR 9 cut(s) 30, 247, 272, 313, 324, 384, 397, 430, 520
FaqI GGGAC 1 cut(s) 594
FatI CATG 6 cut(s) 245, 311, 322, 382, 395, 518
Fnu4HI GCNGC 2 cut(s) 327, 363
FokI GGATG 2 cut(s) 454, 667
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 327, 363
FspBI CTAG 1 cut(s) 168
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 4 cut(s) 18, 54, 263, 550
GluI GCNGC 2 cut(s) 327, 363
GsuI CTGGAG 1 cut(s) 698
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 59, 702
HapII CCGG 4 cut(s) 56, 75, 189, 469
HgaI GACGC 1 cut(s) 591
HhaI GCGC 4 cut(s) 19, 55, 264, 551
Hin1I GRCGYC 1 cut(s) 583
Hin1II CATG 6 cut(s) 249, 315, 326, 386, 399, 522
Hin6I GCGC 4 cut(s) 17, 53, 262, 549
HinP1I GCGC 4 cut(s) 17, 53, 262, 549
HincII GTYRAC 2 cut(s) 391, 622
HindII GTYRAC 2 cut(s) 391, 622
HinfI GANTC 1 cut(s) 668
HpaII CCGG 4 cut(s) 56, 75, 189, 469
HphI GGTGA 4 cut(s) 119, 498, 506, 669
Hpy166II GTNNAC 3 cut(s) 118, 391, 622
Hpy188I TCNGA 1 cut(s) 592
Hpy188III TCNNGA 2 cut(s) 638, 715
Hpy8I GTNNAC 3 cut(s) 118, 391, 622
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 3 cut(s) 37, 338, 571
HpyCH4III ACNGT 2 cut(s) 510, 616
HpyCH4V TGCA 6 cut(s) 315, 374, 458, 481, 541, 721
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 88, 362, 371, 478, 555
Hsp92I GRCGYC 1 cut(s) 583
Hsp92II CATG 6 cut(s) 249, 315, 326, 386, 399, 522
HspAI GCGC 4 cut(s) 17, 53, 262, 549
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Kzo9I GATC 2 cut(s) 46, 709
Lsp1109I GCAGC 2 cut(s) 313, 374
LweI GCATC 3 cut(s) 432, 594, 708
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 125, 512
MalI GATC 2 cut(s) 48, 711
MboI GATC 2 cut(s) 46, 709
MboII GAAGA 3 cut(s) 221, 370, 496
MhlI GDGCHC 2 cut(s) 84, 567
MlsI TGGCCA 1 cut(s) 702
MluCI AATT 3 cut(s) 192, 405, 595
MluNI TGGCCA 1 cut(s) 702
MlyI GAGTC 1 cut(s) 662
MnlI CCTC 5 cut(s) 55, 412, 596, 639, 694
Mox20I TGGCCA 1 cut(s) 702
Mph1103I ATGCAT 1 cut(s) 317
MroNI GCCGGC 1 cut(s) 55
MscI TGGCCA 1 cut(s) 702
MseI TTAA 2 cut(s) 69, 368
Msp20I TGGCCA 1 cut(s) 702
MspI CCGG 4 cut(s) 56, 75, 189, 469
MspR9I CCNGG 2 cut(s) 75, 665
MvaI CCWGG 1 cut(s) 665
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 6 cut(s) 79, 88, 362, 371, 478, 555
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 75
NcoI CCATGG 2 cut(s) 382, 395
NdeII GATC 2 cut(s) 46, 709
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 6 cut(s) 249, 315, 326, 386, 399, 522
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 125, 512
NsbI TGCGCA 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 317
PdiI GCCGGC 1 cut(s) 57
PkrI GCNGC 2 cut(s) 328, 364
PleI GAGTC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 662
PshAI GACNNNNGTC 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 663
PspGI CCWGG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 69, 368
SatI GCNGC 2 cut(s) 327, 363
Sau3AI GATC 2 cut(s) 46, 709
SchI GAGTC 1 cut(s) 662
ScrFI CCNGG 2 cut(s) 75, 665
SduI GDGCHC 2 cut(s) 84, 567
SetI ASST 7 cut(s) 39, 84, 125, 158, 349, 463, 631
SfaNI GCATC 3 cut(s) 432, 594, 708
SfcI CTRYAG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 630
SmoI CTYRAG 1 cut(s) 630
Sse9I AATT 3 cut(s) 192, 405, 595
SsiI CCGC 3 cut(s) 5, 25, 135
SspMI CTAG 1 cut(s) 168
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 73, 663
StyI CCWWGG 4 cut(s) 179, 299, 382, 395
TaaI ACNGT 2 cut(s) 510, 616
TaqI TCGA 2 cut(s) 96, 110
TasI AATT 3 cut(s) 192, 405, 595
Tru1I TTAA 2 cut(s) 69, 368
Tru9I TTAA 2 cut(s) 69, 368
TscAI CASTG 1 cut(s) 548
TseFI GTSAC 2 cut(s) 125, 512
TseI GCWGC 2 cut(s) 326, 362
Tsp45I GTSAC 2 cut(s) 125, 512
TspDTI ATGAA 2 cut(s) 590, 659
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 1 cut(s) 548
XapI RAATTY 1 cut(s) 192
XspI CTAG 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.