MD13G1258300.v1.1

Cell number regulator 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007068
Physical Location & Seq
Reverse (-)
30948076 .. 30948640
565 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1258300.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGCGGACGGAACTGCGCACTCGCGGTATGTGAAGCTGACAAAGGATCAAGCGCCGGCCGAGGACATTAAGCCCGGCGAGCTCAATCGGCCCATCGAAGTTCCCCAGTTGAATGTTCACAGGTGTCATGAATGCGGACAACCTTTACCCGAGAGCTATCAGCCCCCTGGAGATGAGCCTTGGACAACCGGAATTTTTGGCTGTGCTGAAGATAGAGAAAGTTGCTTGACAGGGTTATTTTGTCCATGTGTTCTGTTTGGGCGCAATGTTGAGAGCCTAAGAGACGATACCCCTTGGAACAGACCATGCATTTGTCATGCCGTTTGTATTGAAGGTGGTATGGCTCTGGCAGCATTAACTGCAATCTTCCATGACATTGACCCAAGGACTTCATTTCTTATTTGCGAGTGTCTGTTATTTTCTTGGTGGATGTGTGGGGTATATACAGGTCTTGTCTTGCAATCCTTGCAGAAGAAGTATCATCTACAGAACTCGCCGTGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

169

Amino Acids

18.89

Weight (kDa)

5.44

Isoelectric Point (pI)

56.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 61 - 166 1.1e-14 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 18
AccII CGCG 1 cut(s) 25
AciI CCGC 3 cut(s) 5, 25, 135
AclWI GGATC 1 cut(s) 54
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 228
AgsI TTSAA 2 cut(s) 112, 332
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 3 cut(s) 37, 82, 156
AluI AGCT 3 cut(s) 37, 82, 156
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 1 cut(s) 54
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 57, 89
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 1 cut(s) 192
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
AspLEI GCGC 3 cut(s) 19, 55, 264
AspS9I GGNCC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 75
AvaI CYCGRG 1 cut(s) 149
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 362
BccI CCATC 1 cut(s) 101
BceAI ACGGC 2 cut(s) 305, 481
BciT130I CCWGG 1 cut(s) 168
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 485
BfoI RGCGCY 1 cut(s) 56
BisI GCNGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 352
Bme1390I CCNGG 2 cut(s) 75, 168
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 90
BmrFI CCNGG 2 cut(s) 75, 168
BmrI ACTGGG 1 cut(s) 100
BmuI ACTGGG 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 189
BpuMI CCSGG 1 cut(s) 75
BsaJI CCNNGG 6 cut(s) 60, 166, 179, 293, 383, 497
BsaWI WCCGGW 1 cut(s) 188
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 106
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 6 cut(s) 60, 166, 179, 293, 383, 497
BseGI GGATG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 271
BseNI ACTGG 1 cut(s) 106
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 25
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 91
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 3 cut(s) 56, 75, 189
BsmAI GTCTC 1 cut(s) 276
BsmBI CGTCTC 1 cut(s) 276
BsmI GAATGC 1 cut(s) 137
BsnI GGCC 2 cut(s) 59, 91
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 1 cut(s) 46
BspACI CCGC 3 cut(s) 5, 25, 135
BspANI GGCC 2 cut(s) 59, 91
BspFNI CGCG 1 cut(s) 25
BspHI TCATGA 1 cut(s) 127
BspPI GGATC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 106
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 6 cut(s) 60, 166, 179, 293, 383, 497
BssMI GATC 1 cut(s) 46
BssT1I CCWWGG 3 cut(s) 179, 293, 383
Bst2UI CCWGG 1 cut(s) 168
BstAPI GCANNNNNTGC 2 cut(s) 359, 466
BstC8I GCNNGC 2 cut(s) 57, 80
BstDEI CTNAG 1 cut(s) 278
BstDSI CCRYGG 1 cut(s) 497
BstF5I GGATG 1 cut(s) 435
BstFNI CGCG 1 cut(s) 25
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 3 cut(s) 19, 55, 264
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 1 cut(s) 46
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 5 cut(s) 79, 88, 350, 359, 466
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 2 cut(s) 73, 166
BstSFI CTRYAG 1 cut(s) 485
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 1 cut(s) 362
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 91
BtgI CCRYGG 1 cut(s) 497
BtsCI GGATG 1 cut(s) 435
Cac8I GCNNGC 2 cut(s) 57, 80
CciI TCATGA 1 cut(s) 127
CfoI GCGC 3 cut(s) 19, 55, 264
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 90
CviAII CATG 5 cut(s) 128, 246, 306, 317, 371
DdeI CTNAG 1 cut(s) 278
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 57
EagI CGGCCG 1 cut(s) 57
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 3 cut(s) 179, 293, 383
Eco24I GRGCYC 1 cut(s) 84
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 3 cut(s) 179, 293, 383
EcoT22I ATGCAT 1 cut(s) 311
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 179, 293, 383
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 5 cut(s) 131, 249, 309, 320, 374
FaiI YATR 9 cut(s) 30, 129, 247, 307, 318, 341, 372, 442, 444
FatI CATG 5 cut(s) 127, 245, 305, 316, 370
Fnu4HI GCNGC 1 cut(s) 351
FokI GGATG 1 cut(s) 442
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 351
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 3 cut(s) 18, 54, 263
GluI GCNGC 1 cut(s) 351
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 59, 91
HapII CCGG 3 cut(s) 56, 75, 189
HhaI GCGC 3 cut(s) 19, 55, 264
Hin1II CATG 5 cut(s) 131, 249, 309, 320, 374
Hin6I GCGC 3 cut(s) 17, 53, 262
HinP1I GCGC 3 cut(s) 17, 53, 262
HpaII CCGG 3 cut(s) 56, 75, 189
Hpy166II GTNNAC 1 cut(s) 118
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 1 cut(s) 118
HpyAV CCTTC 1 cut(s) 326
HpyCH4V TGCA 4 cut(s) 309, 362, 460, 469
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 88, 350, 359, 466
HpyF3I CTNAG 1 cut(s) 278
Hsp92II CATG 5 cut(s) 131, 249, 309, 320, 374
HspAI GCGC 3 cut(s) 17, 53, 262
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 46
Lsp1109I GCAGC 1 cut(s) 362
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 3 cut(s) 221, 358, 484
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 1 cut(s) 192
MnlI CCTC 1 cut(s) 55
Mph1103I ATGCAT 1 cut(s) 311
MroNI GCCGGC 1 cut(s) 55
MseI TTAA 2 cut(s) 69, 356
MspI CCGG 3 cut(s) 56, 75, 189
MspR9I CCNGG 2 cut(s) 75, 168
Mva1269I GAATGC 1 cut(s) 137
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 5 cut(s) 79, 88, 350, 359, 466
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 75
NdeII GATC 1 cut(s) 46
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 5 cut(s) 131, 249, 309, 320, 374
NmeAIII GCCGAG 1 cut(s) 85
NsbI TGCGCA 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 311
PagI TCATGA 1 cut(s) 127
PctI GAATGC 1 cut(s) 137
PdiI GCCGGC 1 cut(s) 57
PkrI GCNGC 1 cut(s) 352
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspPI GGNCC 1 cut(s) 90
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 69, 356
SatI GCNGC 1 cut(s) 351
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 75, 168
SduI GDGCHC 1 cut(s) 84
SetI ASST 7 cut(s) 39, 84, 125, 145, 158, 337, 451
SfcI CTRYAG 1 cut(s) 485
Sse9I AATT 1 cut(s) 192
SsiI CCGC 3 cut(s) 5, 25, 135
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 73, 166
StyI CCWWGG 3 cut(s) 179, 293, 383
TaqI TCGA 1 cut(s) 96
TasI AATT 1 cut(s) 192
Tru1I TTAA 2 cut(s) 69, 356
Tru9I TTAA 2 cut(s) 69, 356
TseI GCWGC 1 cut(s) 350
TspDTI ATGAA 2 cut(s) 144, 381
TspGWI ACGGA 1 cut(s) 24
XapI RAATTY 1 cut(s) 192
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.