MD07G1253200.v1.1

Cell number regulator 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007068
Physical Location & Seq
Reverse (-)
32065186 .. 32067643
2458 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1253200.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGCGGACGGAACTGCGCACTCGCGGTATGTGAAGCTGACGAAGGATCAAGCGCCGGCCGAGGACATTAAGCCCGGCGAGCTCAATCAGCCCATCGAAGTTCCCCAGTTGAATGTTCACAGGTGTCACGAATGTGGACAGCCTTTACCCGAGAGCTATCAGCCCCCTGGAGATGAGCCTTGGACAACCGGAATTTTTGGCTGTGCTGAAGATAGAGAAAGTTGCTTGACAGGGTTATTTTGTCCATGTGTTCTGTTTGGGCGCAATGTTGAGAGCCTAAGAGACGATACCCCTTGGAACAGACCATGCATTTGTCATGCCGTTTGTATTGAAGGTGGTATGGCTCTGGCAGCATTAACTGCAATCTTCCATGGCATTGACCCAAGGACTTCATTTCTTATTTGCGAGGGTCTGTTATTTTCTTGGTGGATGTGTGGGGTATATACAGGTCTTGTCCGGCAATCCTTGCAGAAGAAGTATCATCTACAGAACTCGCCGTGTGACCCATGCTTGACACACTGCTGCTTGCACTGGTGCGCCTTGTGCCAAGAGCACAGGGAGATGAAGGGACGCCTCTCGGACAATGCTGTGATGCCAATGACCGTCGTCAACCCACCCCAAGTTCAACAGATGAAGTCTGCTGACGACAACCAGGACTCCGCTTCACCCTCCTCTACCAACAACAATGGCCACACAGATATGGAGATGCAGGCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

240

Amino Acids

26.55

Weight (kDa)

5.27

Isoelectric Point (pI)

45.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 61 - 187 3.9e-27 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 18
AccII CGCG 1 cut(s) 25
AciI CCGC 3 cut(s) 5, 25, 660
AclWI GGATC 1 cut(s) 54
AcoI YGGCCR 2 cut(s) 57, 688
AcsI RAATTY 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 228
AcyI GRCGYC 1 cut(s) 571
AgsI TTSAA 3 cut(s) 112, 332, 626
AjnI CCWGG 2 cut(s) 166, 651
AleI CACNNNNGTG 1 cut(s) 132
AluBI AGCT 3 cut(s) 37, 82, 156
AluI AGCT 3 cut(s) 37, 82, 156
Alw21I GWGCWC 2 cut(s) 84, 555
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 1 cut(s) 54
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 2 cut(s) 57, 688
ApeKI GCWGC 2 cut(s) 350, 522
ApoI RAATTY 1 cut(s) 192
ArsI GACNNNNNNTTYG 2 cut(s) 294, 326
AspLEI GCGC 4 cut(s) 19, 55, 264, 539
AsuC2I CCSGG 1 cut(s) 75
AsuHPI GGTGA 1 cut(s) 657
AvaI CYCGRG 1 cut(s) 149
BalI TGGCCA 1 cut(s) 690
BanII GRGCYC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 84, 555
BbvI GCAGC 2 cut(s) 362, 509
BccI CCATC 1 cut(s) 101
BceAI ACGGC 2 cut(s) 305, 481
BciT130I CCWGG 2 cut(s) 168, 653
BcnI CCSGG 1 cut(s) 75
BcoDI GTCTC 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 485
BfoI RGCGCY 1 cut(s) 56
BisI GCNGC 2 cut(s) 351, 523
BlsI GCNGC 2 cut(s) 352, 524
Bme1390I CCNGG 3 cut(s) 75, 168, 653
BmeT110I CYCGRG 1 cut(s) 149
BmrFI CCNGG 3 cut(s) 75, 168, 653
BmrI ACTGGG 1 cut(s) 100
BmsI GCATC 2 cut(s) 582, 696
BmuI ACTGGG 1 cut(s) 100
BoxI GACNNNNGTC 1 cut(s) 605
BpmI CTGGAG 1 cut(s) 189
BpuMI CCSGG 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 571
BsaJI CCNNGG 6 cut(s) 60, 166, 179, 293, 370, 383
BsaWI WCCGGW 1 cut(s) 188
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 2 cut(s) 106, 536
Bse3DI GCAATG 1 cut(s) 271
BseBI CCWGG 2 cut(s) 168, 653
BseDI CCNNGG 6 cut(s) 60, 166, 179, 293, 370, 383
BseGI GGATG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 271
BseNI ACTGG 2 cut(s) 106, 536
BseRI GAGGAG 1 cut(s) 661
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 2 cut(s) 362, 509
Bsh1236I CGCG 1 cut(s) 25
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 59, 690
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 84, 555
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 4 cut(s) 56, 75, 189, 457
BslFI GGGAC 1 cut(s) 582
BsmAI GTCTC 1 cut(s) 276
BsmBI CGTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 582
BsnI GGCC 2 cut(s) 59, 690
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 84, 555
Bsp143I GATC 1 cut(s) 46
Bsp19I CCATGG 1 cut(s) 370
BspACI CCGC 3 cut(s) 5, 25, 660
BspANI GGCC 2 cut(s) 59, 690
BspFNI CGCG 1 cut(s) 25
BspPI GGATC 1 cut(s) 54
BsrDI GCAATG 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 2 cut(s) 106, 536
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 6 cut(s) 60, 166, 179, 293, 370, 383
BssMI GATC 1 cut(s) 46
BssNI GRCGYC 1 cut(s) 571
BssT1I CCWWGG 4 cut(s) 179, 293, 370, 383
Bst2UI CCWGG 2 cut(s) 168, 653
Bst4CI ACNGT 1 cut(s) 604
BstACI GRCGYC 1 cut(s) 571
BstAPI GCANNNNNTGC 2 cut(s) 359, 466
BstC8I GCNNGC 4 cut(s) 57, 80, 527, 711
BstDEI CTNAG 1 cut(s) 278
BstDSI CCRYGG 1 cut(s) 370
BstF5I GGATG 1 cut(s) 435
BstFNI CGCG 1 cut(s) 25
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 4 cut(s) 19, 55, 264, 539
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 1 cut(s) 46
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 6 cut(s) 79, 88, 350, 359, 466, 543
BstNI CCWGG 2 cut(s) 168, 653
BstPAI GACNNNNGTC 1 cut(s) 605
BstSCI CCNGG 3 cut(s) 73, 166, 651
BstSFI CTRYAG 1 cut(s) 485
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 2 cut(s) 362, 509
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 59, 690
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 1 cut(s) 435
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 2 cut(s) 517, 529
Cac8I GCNNGC 4 cut(s) 57, 80, 527, 711
CfoI GCGC 4 cut(s) 19, 55, 264, 539
Cfr10I RCCGGY 1 cut(s) 55
CseI GACGC 1 cut(s) 579
CviAII CATG 5 cut(s) 246, 306, 317, 371, 507
DdeI CTNAG 1 cut(s) 278
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
EaeI YGGCCR 2 cut(s) 57, 688
EagI CGGCCG 1 cut(s) 57
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 82
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 4 cut(s) 179, 293, 370, 383
Eco24I GRGCYC 1 cut(s) 84
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 166, 651
EcoT14I CCWWGG 4 cut(s) 179, 293, 370, 383
EcoT22I ATGCAT 1 cut(s) 311
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 4 cut(s) 179, 293, 370, 383
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 5 cut(s) 249, 309, 320, 374, 510
FaqI GGGAC 1 cut(s) 582
FatI CATG 5 cut(s) 245, 305, 316, 370, 506
Fnu4HI GCNGC 2 cut(s) 351, 523
FokI GGATG 1 cut(s) 442
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 351, 523
FspI TGCGCA 1 cut(s) 18
GlaI GCGC 4 cut(s) 18, 54, 263, 538
GluI GCNGC 2 cut(s) 351, 523
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 59, 690
HapII CCGG 4 cut(s) 56, 75, 189, 457
HgaI GACGC 1 cut(s) 579
HhaI GCGC 4 cut(s) 19, 55, 264, 539
Hin1I GRCGYC 1 cut(s) 571
Hin1II CATG 5 cut(s) 249, 309, 320, 374, 510
Hin6I GCGC 4 cut(s) 17, 53, 262, 537
HinP1I GCGC 4 cut(s) 17, 53, 262, 537
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 1 cut(s) 656
HpaII CCGG 4 cut(s) 56, 75, 189, 457
HphI GGTGA 1 cut(s) 657
Hpy166II GTNNAC 3 cut(s) 118, 137, 610
Hpy188I TCNGA 1 cut(s) 580
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 3 cut(s) 118, 137, 610
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 3 cut(s) 37, 326, 559
HpyCH4III ACNGT 1 cut(s) 604
HpyCH4V TGCA 5 cut(s) 309, 362, 469, 529, 709
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 88, 350, 359, 466, 543
HpyF3I CTNAG 1 cut(s) 278
Hsp92I GRCGYC 1 cut(s) 571
Hsp92II CATG 5 cut(s) 249, 309, 320, 374, 510
HspAI GCGC 4 cut(s) 17, 53, 262, 537
KroI GCCGGC 1 cut(s) 55
KroNI GCCGGC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 46
Lsp1109I GCAGC 2 cut(s) 362, 509
LweI GCATC 2 cut(s) 582, 696
MaeIII GTNAC 2 cut(s) 125, 500
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 3 cut(s) 221, 358, 484
MhlI GDGCHC 2 cut(s) 84, 555
MlsI TGGCCA 1 cut(s) 690
MluCI AATT 1 cut(s) 192
MluNI TGGCCA 1 cut(s) 690
MlyI GAGTC 1 cut(s) 650
MnlI CCTC 5 cut(s) 55, 400, 584, 679, 682
Mox20I TGGCCA 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 311
MroNI GCCGGC 1 cut(s) 55
MscI TGGCCA 1 cut(s) 690
MseI TTAA 2 cut(s) 69, 356
MslI CAYNNNNRTG 2 cut(s) 132, 698
Msp20I TGGCCA 1 cut(s) 690
MspI CCGG 4 cut(s) 56, 75, 189, 457
MspR9I CCNGG 3 cut(s) 75, 168, 653
MvaI CCWGG 2 cut(s) 168, 653
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 6 cut(s) 79, 88, 350, 359, 466, 543
NaeI GCCGGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 75
NcoI CCATGG 1 cut(s) 370
NdeII GATC 1 cut(s) 46
NgoMIV GCCGGC 1 cut(s) 55
NlaIII CATG 5 cut(s) 249, 309, 320, 374, 510
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 125, 500
NsbI TGCGCA 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 311
OliI CACNNNNGTG 1 cut(s) 132
PdiI GCCGGC 1 cut(s) 57
PkrI GCNGC 2 cut(s) 352, 524
PleI GAGTC 1 cut(s) 650
PpsI GAGTC 1 cut(s) 650
PshAI GACNNNNGTC 1 cut(s) 605
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 2 cut(s) 166, 651
PspGI CCWGG 2 cut(s) 166, 651
RseI CAYNNNNRTG 2 cut(s) 132, 698
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 69, 356
SatI GCNGC 2 cut(s) 351, 523
Sau3AI GATC 1 cut(s) 46
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 3 cut(s) 75, 168, 653
SduI GDGCHC 2 cut(s) 84, 555
SetI ASST 6 cut(s) 39, 84, 125, 158, 337, 451
SfaNI GCATC 2 cut(s) 582, 696
SfcI CTRYAG 1 cut(s) 485
SmiMI CAYNNNNRTG 2 cut(s) 132, 698
Sse9I AATT 1 cut(s) 192
SsiI CCGC 3 cut(s) 5, 25, 660
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 3 cut(s) 73, 166, 651
StyI CCWWGG 4 cut(s) 179, 293, 370, 383
TaaI ACNGT 1 cut(s) 604
TaqI TCGA 1 cut(s) 96
TasI AATT 1 cut(s) 192
Tru1I TTAA 2 cut(s) 69, 356
Tru9I TTAA 2 cut(s) 69, 356
TscAI CASTG 2 cut(s) 524, 536
TseFI GTSAC 2 cut(s) 125, 500
TseI GCWGC 2 cut(s) 350, 522
Tsp45I GTSAC 2 cut(s) 125, 500
TspDTI ATGAA 3 cut(s) 381, 578, 647
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 2 cut(s) 524, 536
XapI RAATTY 1 cut(s) 192
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.