MD01G1228500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014384
Physical Location & Seq
Reverse (-)
31819506 .. 31820315
810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1228500.v1.1.491

Sequence Viewer

Length: 810 bp
ATGTCGCCGCAAGAAGTAGTCAGCAACAACCTCCAAACGCTACCCGATCTCCTCCTGTCGGGGCTCTCCATCTGCTTCCTCTTCTCCTTCTCCGCCGGAGCTACCCTCTTCTCCAAAACCCCATTTCTCCATTTTCCTTCCAACCCTCGGCGCCTATTCAAAATCCCCACCATGTCCCTCCACCACCACCACCAGCAGCACCGCTTCCCCACCCCACAGTCCCTCTCCGAGTGGCTCAAGCCTCGATTGCTCTCCGACTCTCTGGCCTCTTGGGGGGTCAAGCCCGGGACCAAGAACGTCCACAACCTCTGGCTCGAGCTCTCCGAAGGCGAAAGCTCTTTAGCCGATTCATCCCCTCCGGTTCGAACGGTTCAGGTTGTGACGGTACGGATCATCGACAATAAATCCCGGGTTTTGGTCGAATCCCACCAAGAATTATCGGATGGCAGCGTCAGGAGCCGAGGCCGGCCATTGTCAGAGAAAATGAAGCCGGGAGAGGACCACGAATCTGCGGCGAACCGGGCGGTTATGGAAGAGCTCGGTTCAATATTGAATTCGGAAAGCAGCGGAATTGTGAAGATTGTTCCAGGTTCTTATGAGAAGAGGGTGGAGGAGCGGCATTCGATGTCGTACCCGGGGTTGCCGGCGTGTTATGTGCTGCACTCGGTGGATGCTTGGGTGGAGGGCTTGCCGGATGGTGAGTTTTGCACCGGGGAGGAGCACGAGTATTCGGATGGCGGAGCGATGAGCATTGCTCACGAGGCTGTGTACGTCAAGAAGCATTTTTGGAAATGGGTTAGTCTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

270

Amino Acids

30.02

Weight (kDa)

6.16

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014384)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24460
fragaria_vesca FvH4_7g32940
malus_domestica MD01G1228500.v1.1 MD07G1299300.v1.1
prunus_persica Prupe.2G319100_v2.0.a1
pyrus_communis pycom01g23660
rosa_chinensis RchiOBHm_Chr1g0382901
rosa_laevigata RLG00000026150
rosa_multiflora Rmu_co8243775.1_g000001 Rmu_sc0010366.1_g000004
rosa_roxburghii Rroxscaffold_4G00277790
rosa_rugosa Rorug01G0437200
rosa_samantha Rh1AG462200 Rh1BG419100 Rh1CG432900 Rh1DG451300
rosa_wichuraiana Rw1G040960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 150
AccBSI CCGCTC 1 cut(s) 616
AciI CCGC 8 cut(s) 8, 93, 202, 512, 524, 567, 616, 738
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 467
AcsI RAATTY 1 cut(s) 553
AcyI GRCGYC 1 cut(s) 151
AdeI CACNNNGTG 1 cut(s) 667
AfaI GTAC 3 cut(s) 387, 632, 770
AfiI CCNNNNNNNGG 4 cut(s) 58, 147, 273, 415
AgsI TTSAA 3 cut(s) 160, 546, 553
AjnI CCWGG 1 cut(s) 586
AluBI AGCT 4 cut(s) 101, 319, 336, 538
AluI AGCT 4 cut(s) 101, 319, 336, 538
Alw21I GWGCWC 3 cut(s) 321, 540, 723
AlwI GGATC 1 cut(s) 398
Ama87I CYCGRG 4 cut(s) 284, 314, 408, 634
AoxI GGCC 3 cut(s) 264, 463, 467
ApeKI GCWGC 4 cut(s) 196, 447, 564, 658
ApoI RAATTY 1 cut(s) 553
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 2 cut(s) 288, 499
AsuC2I CCSGG 9 cut(s) 285, 286, 409, 410, 492, 521, 635, 636, 712
AsuHPI GGTGA 1 cut(s) 710
AsuII TTCGAA 1 cut(s) 364
AvaI CYCGRG 4 cut(s) 284, 314, 408, 634
AvaII GGWCC 2 cut(s) 288, 499
BanI GGYRCC 1 cut(s) 150
BanII GRGCYC 3 cut(s) 66, 321, 540
BauI CACGAG 2 cut(s) 722, 758
Bbv12I GWGCWC 3 cut(s) 321, 540, 723
BbvI GCAGC 4 cut(s) 208, 459, 576, 645
BccI CCATC 4 cut(s) 77, 437, 689, 728
BciT130I CCWGG 1 cut(s) 588
BcnI CCSGG 9 cut(s) 285, 286, 409, 410, 492, 521, 635, 636, 712
BfoI RGCGCY 1 cut(s) 154
BisI GCNGC 7 cut(s) 8, 197, 448, 513, 565, 617, 659
BlsI GCNGC 7 cut(s) 9, 198, 449, 514, 566, 618, 660
Bme18I GGWCC 2 cut(s) 288, 499
BmeT110I CYCGRG 4 cut(s) 284, 314, 408, 634
BmgT120I GGNCC 2 cut(s) 288, 499
BmiI GGNNCC 3 cut(s) 152, 289, 458
BmsI GCATC 1 cut(s) 661
BplI GAGNNNNNCTC 4 cut(s) 90, 122, 739, 771
Bpu14I TTCGAA 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 9 cut(s) 285, 286, 409, 410, 492, 521, 635, 636, 712
BsaHI GRCGYC 1 cut(s) 151
BsaJI CCNNGG 7 cut(s) 146, 284, 408, 460, 634, 635, 711
BsaWI WCCGGW 1 cut(s) 358
BsaXI ACNNNNNCTCC 4 cut(s) 33, 63, 710, 740
Bsc4I CCNNNNNNNGG 4 cut(s) 58, 147, 273, 415
Bse118I RCCGGY 2 cut(s) 465, 643
Bse3DI GCAATG 1 cut(s) 750
BseBI CCWGG 1 cut(s) 588
BseDI CCNNGG 7 cut(s) 146, 284, 408, 460, 634, 635, 711
BseGI GGATG 5 cut(s) 350, 448, 676, 700, 739
BseLI CCNNNNNNNGG 4 cut(s) 58, 147, 273, 415
BseMI GCAATG 1 cut(s) 750
BseRI GAGGAG 3 cut(s) 41, 626, 731
BseXI GCAGC 4 cut(s) 208, 459, 576, 645
BsgI GTGCAG 1 cut(s) 644
BshFI GGCC 3 cut(s) 266, 465, 469
BshNI GGYRCC 1 cut(s) 150
BsiHKAI GWGCWC 3 cut(s) 321, 540, 723
BsiHKCI CYCGRG 4 cut(s) 284, 314, 408, 634
BslFI GGGAC 3 cut(s) 160, 205, 301
BslI CCNNNNNNNGG 4 cut(s) 58, 147, 273, 415
BsmFI GGGAC 3 cut(s) 160, 205, 301
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 3 cut(s) 266, 465, 469
BsoBI CYCGRG 4 cut(s) 284, 314, 408, 634
Bsp119I TTCGAA 1 cut(s) 364
Bsp1286I GDGCHC 4 cut(s) 66, 321, 540, 723
Bsp143I GATC 2 cut(s) 46, 390
BspACI CCGC 8 cut(s) 8, 93, 202, 512, 524, 567, 616, 738
BspANI GGCC 3 cut(s) 266, 465, 469
BspLI GGNNCC 3 cut(s) 152, 289, 458
BspPI GGATC 1 cut(s) 398
BspQI GCTCTTC 1 cut(s) 528
BspT104I TTCGAA 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 150
BsrBI CCGCTC 1 cut(s) 616
BsrDI GCAATG 1 cut(s) 750
BsrFI RCCGGY 2 cut(s) 465, 643
BssAI RCCGGY 2 cut(s) 465, 643
BssECI CCNNGG 7 cut(s) 146, 284, 408, 460, 634, 635, 711
BssMI GATC 2 cut(s) 46, 390
BssNI GRCGYC 1 cut(s) 151
BssSI CACGAG 2 cut(s) 722, 758
Bst2BI CACGAG 2 cut(s) 722, 758
Bst2UI CCWGG 1 cut(s) 588
Bst4CI ACNGT 3 cut(s) 219, 370, 385
Bst6I CTCTTC 4 cut(s) 86, 113, 528, 596
BstACI GRCGYC 1 cut(s) 151
BstBI TTCGAA 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 467, 645, 689
BstF5I GGATG 5 cut(s) 350, 448, 676, 700, 739
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 2 cut(s) 49, 393
BstMBI GATC 2 cut(s) 46, 390
BstMWI GCNNNNNNNGC 4 cut(s) 247, 456, 521, 761
BstNI CCWGG 1 cut(s) 588
BstV1I GCAGC 4 cut(s) 208, 459, 576, 645
BsuRI GGCC 3 cut(s) 266, 465, 469
BtgZI GCGATG 1 cut(s) 758
BtsCI GGATG 5 cut(s) 350, 448, 676, 700, 739
Cac8I GCNNGC 3 cut(s) 467, 645, 689
CfoI GCGC 1 cut(s) 153
Cfr10I RCCGGY 2 cut(s) 465, 643
Cfr13I GGNCC 2 cut(s) 288, 499
Cfr9I CCCGGG 3 cut(s) 284, 408, 634
CseI GACGC 1 cut(s) 439
Csp6I GTAC 3 cut(s) 386, 631, 769
CviAII CATG 1 cut(s) 172
CviQI GTAC 3 cut(s) 386, 631, 769
DinI GGCGCC 1 cut(s) 152
DpnI GATC 2 cut(s) 48, 392
DpnII GATC 2 cut(s) 46, 390
DraIII CACNNNGTG 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 467
Eam1104I CTCTTC 4 cut(s) 86, 113, 528, 596
EarI CTCTTC 4 cut(s) 86, 113, 528, 596
EciI GGCGGA 2 cut(s) 82, 753
Ecl136II GAGCTC 2 cut(s) 319, 538
Eco24I GRGCYC 3 cut(s) 66, 321, 540
Eco47I GGWCC 2 cut(s) 288, 499
Eco53kI GAGCTC 2 cut(s) 319, 538
Eco88I CYCGRG 4 cut(s) 284, 314, 408, 634
EcoICRI GAGCTC 2 cut(s) 319, 538
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 586
EcoT38I GRGCYC 3 cut(s) 66, 321, 540
EgeI GGCGCC 1 cut(s) 152
EheI GGCGCC 1 cut(s) 152
FaeI CATG 1 cut(s) 175
FaiI YATR 4 cut(s) 173, 530, 597, 654
FaqI GGGAC 3 cut(s) 160, 205, 301
FatI CATG 1 cut(s) 171
Fnu4HI GCNGC 7 cut(s) 8, 197, 448, 513, 565, 617, 659
FokI GGATG 5 cut(s) 337, 455, 683, 707, 746
FriOI GRGCYC 3 cut(s) 66, 321, 540
FseI GGCCGGCC 1 cut(s) 469
Fsp4HI GCNGC 7 cut(s) 8, 197, 448, 513, 565, 617, 659
GlaI GCGC 1 cut(s) 152
GluI GCNGC 7 cut(s) 8, 197, 448, 513, 565, 617, 659
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 3 cut(s) 266, 465, 469
HgaI GACGC 1 cut(s) 439
HhaI GCGC 1 cut(s) 153
Hin1I GRCGYC 1 cut(s) 151
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HinfI GANTC 4 cut(s) 257, 347, 422, 506
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 2 cut(s) 301, 769
Hpy188I TCNGA 7 cut(s) 229, 256, 325, 442, 478, 559, 733
Hpy188III TCNNGA 4 cut(s) 454, 758, 775, 803
Hpy8I GTNNAC 2 cut(s) 301, 769
HpyAV CCTTC 3 cut(s) 97, 147, 320
HpyCH4III ACNGT 3 cut(s) 219, 370, 385
HpyCH4IV ACGT 2 cut(s) 297, 771
HpyCH4V TGCA 2 cut(s) 661, 708
HpyF10VI GCNNNNNNNGC 4 cut(s) 247, 456, 521, 761
HpySE526I ACGT 2 cut(s) 297, 771
Hsp92I GRCGYC 1 cut(s) 151
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 1 cut(s) 151
KasI GGCGCC 1 cut(s) 150
KroI GCCGGC 2 cut(s) 465, 643
KroNI GCCGGC 2 cut(s) 467, 645
Kzo9I GATC 2 cut(s) 46, 390
LguI GCTCTTC 1 cut(s) 528
LmnI GCTCC 5 cut(s) 98, 456, 613, 718, 740
Lsp1109I GCAGC 4 cut(s) 208, 459, 576, 645
LweI GCATC 1 cut(s) 661
MaeII ACGT 2 cut(s) 297, 771
MaeIII GTNAC 1 cut(s) 379
MalI GATC 2 cut(s) 48, 392
MbiI CCGCTC 1 cut(s) 616
MboI GATC 2 cut(s) 46, 390
MboII GAAGA 5 cut(s) 73, 100, 545, 589, 613
MhlI GDGCHC 4 cut(s) 66, 321, 540, 723
MluCI AATT 3 cut(s) 434, 553, 570
Mly113I GGCGCC 1 cut(s) 151
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 2 cut(s) 165, 279
MroNI GCCGGC 2 cut(s) 465, 643
MspA1I CMGCKG 1 cut(s) 567
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 1 cut(s) 588
MwoI GCNNNNNNNGC 4 cut(s) 247, 456, 521, 761
NaeI GCCGGC 2 cut(s) 467, 645
NarI GGCGCC 1 cut(s) 151
NciI CCSGG 9 cut(s) 285, 286, 409, 410, 492, 521, 635, 636, 712
NdeII GATC 2 cut(s) 46, 390
NgoMIV GCCGGC 2 cut(s) 465, 643
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 3 cut(s) 152, 289, 458
NmeAIII GCCGAG 2 cut(s) 127, 485
NmuCI GTSAC 1 cut(s) 379
NspV TTCGAA 1 cut(s) 364
PaeR7I CTCGAG 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 528
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PctI GAATGC 1 cut(s) 619
PdiI GCCGGC 2 cut(s) 467, 645
PfeI GAWTC 3 cut(s) 347, 422, 506
PkrI GCNGC 7 cut(s) 9, 198, 449, 514, 566, 618, 660
PleI GAGTC 1 cut(s) 251
PluTI GGCGCC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 251
Psp124BI GAGCTC 2 cut(s) 321, 540
Psp6I CCWGG 1 cut(s) 586
PspGI CCWGG 1 cut(s) 586
PspN4I GGNNCC 3 cut(s) 152, 289, 458
PspPI GGNCC 2 cut(s) 288, 499
PspXI VCTCGAGB 1 cut(s) 314
RigI GGCCGGCC 1 cut(s) 469
RsaI GTAC 3 cut(s) 387, 632, 770
RsaNI GTAC 3 cut(s) 386, 631, 769
SacI GAGCTC 2 cut(s) 321, 540
SapI GCTCTTC 1 cut(s) 528
SatI GCNGC 7 cut(s) 8, 197, 448, 513, 565, 617, 659
Sau3AI GATC 2 cut(s) 46, 390
Sau96I GGNCC 2 cut(s) 288, 499
SchI GAGTC 1 cut(s) 251
SduI GDGCHC 4 cut(s) 66, 321, 540, 723
SfaNI GCATC 1 cut(s) 661
SfoI GGCGCC 1 cut(s) 152
Sfr274I CTCGAG 1 cut(s) 314
SfuI TTCGAA 1 cut(s) 364
SinI GGWCC 2 cut(s) 288, 499
SlaI CTCGAG 1 cut(s) 314
SmaI CCCGGG 3 cut(s) 286, 410, 636
SmlI CTYRAG 3 cut(s) 236, 314, 803
SmoI CTYRAG 3 cut(s) 236, 314, 803
Sse9I AATT 3 cut(s) 434, 553, 570
SsiI CCGC 8 cut(s) 8, 93, 202, 512, 524, 567, 616, 738
SspDI GGCGCC 1 cut(s) 150
SspI AATATT 1 cut(s) 549
SstI GAGCTC 2 cut(s) 321, 540
TaaI ACNGT 3 cut(s) 219, 370, 385
TaiI ACGT 2 cut(s) 300, 774
TaqI TCGA 6 cut(s) 244, 315, 364, 396, 420, 623
TasI AATT 3 cut(s) 434, 553, 570
TauI GCSGC 3 cut(s) 10, 515, 619
TfiI GAWTC 3 cut(s) 347, 422, 506
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 4 cut(s) 196, 447, 564, 658
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 339, 500
TspGWI ACGGA 1 cut(s) 403
TspMI CCCGGG 3 cut(s) 284, 408, 634
VpaK11BI GGWCC 2 cut(s) 288, 499
XapI RAATTY 1 cut(s) 553
XhoI CTCGAG 1 cut(s) 314
XmaI CCCGGG 3 cut(s) 284, 408, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.