Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005638

Physical Location & Seq

Genomic Coordinates Forward (+)

682393 .. 685304

Gene Structure 2912 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1010400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 600 bp

ATGAAGATCACGGCTTTGCTGGTCCTCAAGTGCAATCCAGACGAATCCGACCCCATCATCTTGGCCAACGCCTCCGATACCAGTCACTTCGGCTACTTCCAGAGGTCCAGCGTCAAGGAGTTCATCGTCTTCGTCGGTCGCACCGTCGCCAGACGCACCCCGCCTGGCCAACGCCAGTCCGTCAAGCACGAAGAGTATAAAGTACATGCTTACAACAGCAATGGTCTCTGTGCGCTGGGCTTTATGGATGATCACTATCCCGTTCGAAGTGCATTTTCTCTGCTCAACCAGGTGCTAGATGAATATCAGAAAAAATTTGGTGATTCTTGGAGGACTGCAAAACAGGATGGCACACAACCATGGCCTTATTTGAGTGAGGCTCTGACGAAATTCCAGGATCCTGCAGAGGCTGATAAGTTGTTGAAAATACAAAGGGAATTGGATGAAACAAAAATTATCCTTCATAAGACTATTGATAGTGTGCTTGCACGAGGTGAGAGACTGGACAGTTTAGTTGAGAAGAGTTCAGATCTTAGTGCAGCATCGCAGATGTTCTACAAGCAGGCAAAAAAGACCAATCAGTGTTGTACCATATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006887 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0032940 GO:0032991 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048284 GO:0051179 GO:0051234 GO:0061024 GO:0061025 GO:0071840 GO:0090174 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04130 ko04138 map04130 map04138

Pfam Domains

Protein Families

Longin Synaptobrevin

Protein Analysis

200

Amino Acids

22.61

Weight (kDa)

8.27

Isoelectric Point (pI)

36.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	47 - 111	4.5e-15	Regulated-SNARE-like domain
Synaptobrevin	PF00957	140 - 194	6.7e-14	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005638)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58060 AT5G58060 AT5G58180 AT5G58180
fragaria_vesca	FvH4_1g01250
malus_domestica	MD02G1010400.v1.1 MD15G1155400.v1.1
prunus_persica	Prupe.7G259700_v2.0.a1
pyrus_communis	pycom02g00930 pycom15g13940
rosa_chinensis	RchiOBHm_Chr2g0086021 RchiOBHm_Chr5g0044951
rosa_laevigata	RLG00000015735 RLG00000019563
rosa_multiflora	Rmu_sc0009954.1_g000007 Rmu_sc0011619.1_g000001 Rmu_sc0012115.1_g000001 Rmu_sc0016521.1_g000001 Rmu_ssc0000355.1_g000022
rosa_roxburghii	Rroxscaffold_2G00154840
rosa_rugosa	Rorug01G0465200
rosa_samantha	Rh2AG014400 Rh2BG014900 Rh2CG015500 Rh2DG016800 Rh5BG308700 Rh5DG319100
rosa_wichuraiana	Rw2G001280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	161
AclWI	GGATC	2 cut(s)	392, 405
AcoI	YGGCCR	2 cut(s)	63, 166
AcsI	RAATTY	2 cut(s)	314, 389
AdeI	CACNNNGTG	1 cut(s)	494
AfaI	GTAC	2 cut(s)	204, 589
AgsI	TTSAA	1 cut(s)	424
AjnI	CCWGG	3 cut(s)	163, 288, 393
Alw26I	GTCTC	2 cut(s)	230, 493
AlwI	GGATC	2 cut(s)	392, 405
AlwNI	CAGNNNCTG	1 cut(s)	410
AoxI	GGCC	3 cut(s)	63, 166, 362
ApeKI	GCWGC	1 cut(s)	539
ApoI	RAATTY	2 cut(s)	314, 389
AspLEI	GCGC	1 cut(s)	235
AspS9I	GGNCC	2 cut(s)	22, 105
AsuHPI	GGTGA	2 cut(s)	332, 506
AsuII	TTCGAA	1 cut(s)	265
AvaII	GGWCC	2 cut(s)	22, 105
BalI	TGGCCA	2 cut(s)	65, 168
BamHI	GGATCC	1 cut(s)	397
BauI	CACGAG	1 cut(s)	489
BbsI	GAAGAC	1 cut(s)	121
BbvI	GCAGC	1 cut(s)	551
BccI	CCATC	2 cut(s)	62, 341
BceAI	ACGGC	1 cut(s)	27
BciT130I	CCWGG	3 cut(s)	165, 290, 395
BclI	TGATCA	1 cut(s)	250
BcoDI	GTCTC	2 cut(s)	230, 493
BfaI	CTAG	1 cut(s)	296
BfmI	CTRYAG	1 cut(s)	402
BglII	AGATCT	1 cut(s)	529
BisI	GCNGC	1 cut(s)	540
BlsI	GCNGC	1 cut(s)	541
Bme1390I	CCNGG	3 cut(s)	165, 290, 395
Bme18I	GGWCC	2 cut(s)	22, 105
BmgT120I	GGNCC	2 cut(s)	22, 105
BmiI	GGNNCC	1 cut(s)	399
BmrFI	CCNGG	3 cut(s)	165, 290, 395
BmsI	GCATC	1 cut(s)	551
BpiI	GAAGAC	1 cut(s)	121
BplI	GAGNNNNNCTC	2 cut(s)	364, 396
Bpu14I	TTCGAA	1 cut(s)	265
BpuEI	CTTGAG	1 cut(s)	11
BsaBI	GATNNNNATC	2 cut(s)	255, 303
BsaI	GGTCTC	1 cut(s)	230
BsaJI	CCNNGG	1 cut(s)	359
Bse1I	ACTGG	3 cut(s)	81, 175, 507
Bse3DI	GCAATG	1 cut(s)	226
Bse8I	GATNNNNATC	2 cut(s)	255, 303
BseBI	CCWGG	3 cut(s)	165, 290, 395
BseDI	CCNNGG	1 cut(s)	359
BseGI	GGATG	3 cut(s)	253, 352, 448
BseJI	GATNNNNATC	2 cut(s)	255, 303
BseMI	GCAATG	1 cut(s)	226
BseNI	ACTGG	3 cut(s)	81, 175, 507
BseXI	GCAGC	1 cut(s)	551
BseYI	CCCAGC	1 cut(s)	235
BsgI	GTGCAG	1 cut(s)	558
Bsh1285I	CGRYCG	1 cut(s)	139
BshFI	GGCC	3 cut(s)	65, 168, 364
BsiEI	CGRYCG	1 cut(s)	139
BsmAI	GTCTC	2 cut(s)	230, 493
BsnI	GGCC	3 cut(s)	65, 168, 364
Bso31I	GGTCTC	1 cut(s)	230
Bsp119I	TTCGAA	1 cut(s)	265
Bsp143I	GATC	4 cut(s)	6, 250, 397, 529
Bsp19I	CCATGG	1 cut(s)	359
BspACI	CCGC	1 cut(s)	161
BspANI	GGCC	3 cut(s)	65, 168, 364
BspLI	GGNNCC	1 cut(s)	399
BspMAI	CTGCAG	1 cut(s)	406
BspPI	GGATC	2 cut(s)	392, 405
BspT104I	TTCGAA	1 cut(s)	265
BspTNI	GGTCTC	1 cut(s)	230
BsrDI	GCAATG	1 cut(s)	226
BsrI	ACTGG	3 cut(s)	81, 175, 507
BssECI	CCNNGG	1 cut(s)	359
BssMI	GATC	4 cut(s)	6, 250, 397, 529
BssSI	CACGAG	1 cut(s)	489
BssT1I	CCWWGG	1 cut(s)	359
Bst2BI	CACGAG	1 cut(s)	489
Bst2UI	CCWGG	3 cut(s)	165, 290, 395
Bst4CI	ACNGT	2 cut(s)	145, 509
Bst6I	CTCTTC	2 cut(s)	186, 515
BstBI	TTCGAA	1 cut(s)	265
BstC8I	GCNNGC	2 cut(s)	486, 564
BstDEI	CTNAG	1 cut(s)	533
BstDSI	CCRYGG	1 cut(s)	359
BstF5I	GGATG	3 cut(s)	253, 352, 448
BstHHI	GCGC	1 cut(s)	235
BstKTI	GATC	4 cut(s)	9, 253, 400, 532
BstMAI	GTCTC	2 cut(s)	230, 493
BstMBI	GATC	4 cut(s)	6, 250, 397, 529
BstMCI	CGRYCG	1 cut(s)	139
BstNI	CCWGG	3 cut(s)	165, 290, 395
BstNSI	RCATGY	1 cut(s)	209
BstSCI	CCNGG	3 cut(s)	163, 288, 393
BstSFI	CTRYAG	1 cut(s)	402
BstV1I	GCAGC	1 cut(s)	551
BstV2I	GAAGAC	1 cut(s)	121
BstX2I	RGATCY	2 cut(s)	397, 529
BstXI	CCANNNNNNTGG	1 cut(s)	61
BstYI	RGATCY	2 cut(s)	397, 529
BsuRI	GGCC	3 cut(s)	65, 168, 364
BtgI	CCRYGG	1 cut(s)	359
BtgZI	GCGATG	1 cut(s)	528
BtsCI	GGATG	3 cut(s)	253, 352, 448
BtsIMutI	CAGTG	1 cut(s)	587
Cac8I	GCNNGC	2 cut(s)	486, 564
CaiI	CAGNNNCTG	1 cut(s)	410
CfoI	GCGC	1 cut(s)	235
Cfr13I	GGNCC	2 cut(s)	22, 105
CseI	GACGC	2 cut(s)	100, 162
CsiI	ACCWGGT	1 cut(s)	288
Csp6I	GTAC	2 cut(s)	203, 588
CviAII	CATG	2 cut(s)	206, 360
CviJI	RGCY	8 cut(s)	14, 65, 93, 168, 240, 364, 380, 410
CviKI_1	RGCY	8 cut(s)	14, 65, 93, 168, 240, 364, 380, 410
CviQI	GTAC	2 cut(s)	203, 588
DdeI	CTNAG	1 cut(s)	533
DpnI	GATC	4 cut(s)	8, 252, 399, 531
DpnII	GATC	4 cut(s)	6, 250, 397, 529
DraIII	CACNNNGTG	1 cut(s)	494
EaeI	YGGCCR	2 cut(s)	63, 166
Eam1104I	CTCTTC	2 cut(s)	186, 515
EarI	CTCTTC	2 cut(s)	186, 515
Eco130I	CCWWGG	1 cut(s)	359
Eco31I	GGTCTC	1 cut(s)	230
Eco47I	GGWCC	2 cut(s)	22, 105
EcoRII	CCWGG	3 cut(s)	163, 288, 393
EcoT14I	CCWWGG	1 cut(s)	359
ErhI	CCWWGG	1 cut(s)	359
FaeI	CATG	2 cut(s)	209, 363
FaiI	YATR	6 cut(s)	198, 207, 245, 361, 465, 593
FatI	CATG	2 cut(s)	205, 359
FauI	CCCGC	1 cut(s)	168
FbaI	TGATCA	1 cut(s)	250
Fnu4HI	GCNGC	1 cut(s)	540
FokI	GGATG	3 cut(s)	260, 359, 455
Fsp4HI	GCNGC	1 cut(s)	540
FspBI	CTAG	1 cut(s)	296
GlaI	GCGC	1 cut(s)	234
GluI	GCNGC	1 cut(s)	540
GsaI	CCCAGC	1 cut(s)	239
HaeIII	GGCC	3 cut(s)	65, 168, 364
HgaI	GACGC	2 cut(s)	100, 162
HhaI	GCGC	1 cut(s)	235
Hin1II	CATG	2 cut(s)	209, 363
Hin6I	GCGC	1 cut(s)	233
HinP1I	GCGC	1 cut(s)	233
HinfI	GANTC	2 cut(s)	44, 323
HphI	GGTGA	2 cut(s)	332, 506
Hpy188I	TCNGA	5 cut(s)	49, 76, 309, 384, 529
Hpy188III	TCNNGA	2 cut(s)	38, 100
Hpy99I	CGWCG	2 cut(s)	137, 149
HpyAV	CCTTC	1 cut(s)	470
HpyCH4III	ACNGT	2 cut(s)	145, 509
HpyCH4V	TGCA	6 cut(s)	33, 272, 338, 404, 488, 539
HpyF3I	CTNAG	1 cut(s)	533
Hsp92II	CATG	2 cut(s)	209, 363
HspAI	GCGC	1 cut(s)	233
Ksp22I	TGATCA	1 cut(s)	250
Kzo9I	GATC	4 cut(s)	6, 250, 397, 529
Lsp1109I	GCAGC	1 cut(s)	551
LweI	GCATC	1 cut(s)	551
MabI	ACCWGGT	1 cut(s)	288
MaeI	CTAG	1 cut(s)	296
MaeIII	GTNAC	1 cut(s)	83
MalI	GATC	4 cut(s)	8, 252, 399, 531
MboI	GATC	4 cut(s)	6, 250, 397, 529
MboII	GAAGA	4 cut(s)	16, 121, 203, 532
MflI	RGATCY	2 cut(s)	397, 529
MlsI	TGGCCA	2 cut(s)	65, 168
MluCI	AATT	4 cut(s)	314, 389, 437, 453
MluNI	TGGCCA	2 cut(s)	65, 168
MmeI	TCCRAC	1 cut(s)	72
MnlI	CCTC	7 cut(s)	35, 82, 96, 324, 370, 400, 485
Mox20I	TGGCCA	2 cut(s)	65, 168
MscI	TGGCCA	2 cut(s)	65, 168
MslI	CAYNNNNRTG	1 cut(s)	358
Msp20I	TGGCCA	2 cut(s)	65, 168
MspR9I	CCNGG	3 cut(s)	165, 290, 395
MvaI	CCWGG	3 cut(s)	165, 290, 395
NcoI	CCATGG	1 cut(s)	359
NdeII	GATC	4 cut(s)	6, 250, 397, 529
NlaIII	CATG	2 cut(s)	209, 363
NlaIV	GGNNCC	1 cut(s)	399
NmuCI	GTSAC	1 cut(s)	83
NspI	RCATGY	1 cut(s)	209
NspV	TTCGAA	1 cut(s)	265
PcsI	WCGNNNNNNNCGW	1 cut(s)	141
PfeI	GAWTC	2 cut(s)	44, 323
PfoI	TCCNGGA	1 cut(s)	393
PkrI	GCNGC	1 cut(s)	541
Psp6I	CCWGG	3 cut(s)	163, 288, 393
PspFI	CCCAGC	1 cut(s)	235
PspGI	CCWGG	3 cut(s)	163, 288, 393
PspN4I	GGNNCC	1 cut(s)	399
PspPI	GGNCC	2 cut(s)	22, 105
PstI	CTGCAG	1 cut(s)	406
PstNI	CAGNNNCTG	1 cut(s)	410
PsuI	RGATCY	2 cut(s)	397, 529
RsaI	GTAC	2 cut(s)	204, 589
RsaNI	GTAC	2 cut(s)	203, 588
RseI	CAYNNNNRTG	1 cut(s)	358
SatI	GCNGC	1 cut(s)	540
Sau3AI	GATC	4 cut(s)	6, 250, 397, 529
Sau96I	GGNCC	2 cut(s)	22, 105
ScrFI	CCNGG	3 cut(s)	165, 290, 395
SetI	ASST	3 cut(s)	107, 294, 496
SexAI	ACCWGGT	1 cut(s)	288
SfaNI	GCATC	1 cut(s)	551
SfcI	CTRYAG	1 cut(s)	402
SfuI	TTCGAA	1 cut(s)	265
SinI	GGWCC	2 cut(s)	22, 105
SmiMI	CAYNNNNRTG	1 cut(s)	358
SmlI	CTYRAG	1 cut(s)	26
SmoI	CTYRAG	1 cut(s)	26
Sse9I	AATT	4 cut(s)	314, 389, 437, 453
SsiI	CCGC	1 cut(s)	161
SspMI	CTAG	1 cut(s)	296
StyD4I	CCNGG	3 cut(s)	163, 288, 393
StyI	CCWWGG	1 cut(s)	359
TaaI	ACNGT	2 cut(s)	145, 509
TaqI	TCGA	1 cut(s)	265
TaqII	GACCGA	1 cut(s)	125
TasI	AATT	4 cut(s)	314, 389, 437, 453
TatI	WGTACW	1 cut(s)	202
TfiI	GAWTC	2 cut(s)	44, 323
TscAI	CASTG	1 cut(s)	587
TseFI	GTSAC	1 cut(s)	83
TseI	GCWGC	1 cut(s)	539
Tsp45I	GTSAC	1 cut(s)	83
TspDTI	ATGAA	5 cut(s)	17, 112, 315, 452, 459
TspGWI	ACGGA	1 cut(s)	169
TspRI	CASTG	1 cut(s)	587
VpaK11BI	GGWCC	2 cut(s)	22, 105
XapI	RAATTY	2 cut(s)	314, 389
XceI	RCATGY	1 cut(s)	209
XspI	CTAG	1 cut(s)	296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.