Rh2AG014400

Regulated-SNARE-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005638
Physical Location & Seq
Forward (+)
1126482 .. 1129512
3031 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG014400.1

Sequence Viewer

Length: 483 bp
ATGAAGATCACGGCGTTGCTGGTCCTCAAGTGCAACCCGGAAGGATCCGACCCGGTCATATTGGCCAACGCCTCCGATACCTCCCATTTCGGCTACTTCCAGAGGTCCAGCGTCAAGGAGTTCATCGTCTTCGTCGGCCGCACCGTCGCCAAACGCACCCCTCCCGGTCAACGCCAGTCCGTCAAGCACGAAGAGTATAAAGTACATGCTTACAACAGCAATGGACTCTGTGCATTGGGCTTTATGGATGATCACTATCCGGTTAGAAGTGCGTTTTCGCTGCTGAACCAGGTGTTAGATGAATATCAGAAAAAATTTGGTGATTCTTGGAGGACTGCACAACAGGACAGCACAGAATCGTGGCCCTACCTAAATGAGGCTCTAACAAGATTCCAGGTGTGTCTGCAAATCTGTTTTCTTTCTTATTATATAAGCTGGAATCATAAGACATTAAAGTGGATGAAGTTTAATGAGCTCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 ko04138 map04130 map04138
Pfam Domains
Protein Families
Longin Synaptobrevin

Protein Analysis

160

Amino Acids

18.58

Weight (kDa)

8.65

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 47 - 112 1.6e-15 Regulated-SNARE-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 139
AclWI GGATC 2 cut(s) 39, 52
AcoI YGGCCR 2 cut(s) 63, 136
AcsI RAATTY 1 cut(s) 314
AfaI GTAC 1 cut(s) 204
AfiI CCNNNNNNNGG 1 cut(s) 376
AjnI CCWGG 2 cut(s) 288, 393
AluBI AGCT 2 cut(s) 435, 475
AluI AGCT 2 cut(s) 435, 475
Alw21I GWGCWC 1 cut(s) 477
AlwI GGATC 2 cut(s) 39, 52
AoxI GGCC 3 cut(s) 63, 136, 362
ApeKI GCWGC 1 cut(s) 280
ApoI RAATTY 1 cut(s) 314
AspS9I GGNCC 3 cut(s) 22, 105, 363
AsuC2I CCSGG 3 cut(s) 38, 53, 165
AsuHPI GGTGA 1 cut(s) 332
AvaII GGWCC 2 cut(s) 22, 105
BalI TGGCCA 1 cut(s) 65
BamHI GGATCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 1 cut(s) 477
BbvI GCAGC 1 cut(s) 267
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 290, 395
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 3 cut(s) 38, 53, 165
BisI GCNGC 2 cut(s) 139, 281
BlsI GCNGC 2 cut(s) 140, 282
Bme1390I CCNGG 5 cut(s) 38, 53, 165, 290, 395
Bme18I GGWCC 2 cut(s) 22, 105
BmgT120I GGNCC 3 cut(s) 22, 105, 363
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 5 cut(s) 38, 53, 165, 290, 395
BpiI GAAGAC 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 3 cut(s) 38, 53, 165
BsaBI GATNNNNATC 2 cut(s) 255, 303
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 226
Bse8I GATNNNNATC 2 cut(s) 255, 303
BseBI CCWGG 2 cut(s) 290, 395
BseGI GGATG 2 cut(s) 253, 465
BseJI GATNNNNATC 2 cut(s) 255, 303
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 226
BseNI ACTGG 1 cut(s) 175
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 1 cut(s) 267
BsgI GTGCAG 1 cut(s) 321
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 3 cut(s) 65, 138, 364
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 477
BsiSI CCGG 4 cut(s) 38, 53, 165, 260
BslI CCNNNNNNNGG 1 cut(s) 376
BsnI GGCC 3 cut(s) 65, 138, 364
Bsp1286I GDGCHC 1 cut(s) 477
Bsp143I GATC 3 cut(s) 6, 44, 250
BspACI CCGC 1 cut(s) 139
BspANI GGCC 3 cut(s) 65, 138, 364
BspLI GGNNCC 1 cut(s) 46
BspPI GGATC 2 cut(s) 39, 52
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 1 cut(s) 175
BssMI GATC 3 cut(s) 6, 44, 250
Bst2UI CCWGG 2 cut(s) 290, 395
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 186
BstENI CCTNNNNNAGG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 253, 465
BstKTI GATC 3 cut(s) 9, 47, 253
BstMBI GATC 3 cut(s) 6, 44, 250
BstMCI CGRYCG 1 cut(s) 139
BstNI CCWGG 2 cut(s) 290, 395
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 5 cut(s) 36, 51, 163, 288, 393
BstV1I GCAGC 1 cut(s) 267
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
BstZI CGGCCG 1 cut(s) 136
BsuRI GGCC 3 cut(s) 65, 138, 364
BtsCI GGATG 2 cut(s) 253, 465
BtsIMutI CAGTG 1 cut(s) 478
Cfr13I GGNCC 3 cut(s) 22, 105, 363
CseI GACGC 1 cut(s) 100
CsiI ACCWGGT 1 cut(s) 288
Csp6I GTAC 1 cut(s) 203
CviAII CATG 1 cut(s) 206
CviJI RGCY 8 cut(s) 65, 93, 138, 240, 364, 380, 435, 475
CviKI_1 RGCY 8 cut(s) 65, 93, 138, 240, 364, 380, 435, 475
CviQI GTAC 1 cut(s) 203
DpnI GATC 3 cut(s) 8, 46, 252
DpnII GATC 3 cut(s) 6, 44, 250
EaeI YGGCCR 2 cut(s) 63, 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 475
EclXI CGGCCG 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 477
Eco47I GGWCC 2 cut(s) 22, 105
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 475
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoRII CCWGG 2 cut(s) 288, 393
EcoT38I GRGCYC 1 cut(s) 477
FaeI CATG 1 cut(s) 209
FaiI YATR 7 cut(s) 59, 198, 207, 245, 429, 431, 444
FatI CATG 1 cut(s) 205
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 2 cut(s) 139, 281
FokI GGATG 2 cut(s) 260, 472
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 2 cut(s) 139, 281
GluI GCNGC 2 cut(s) 139, 281
HaeIII GGCC 3 cut(s) 65, 138, 364
HapII CCGG 4 cut(s) 38, 53, 165, 260
HgaI GACGC 1 cut(s) 100
Hin1II CATG 1 cut(s) 209
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HinfI GANTC 5 cut(s) 225, 323, 356, 390, 439
HpaII CCGG 4 cut(s) 38, 53, 165, 260
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 3 cut(s) 49, 76, 309
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 2 cut(s) 137, 149
HpyAV CCTTC 1 cut(s) 35
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4V TGCA 4 cut(s) 33, 233, 338, 406
Hsp92II CATG 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 3 cut(s) 6, 44, 250
LmnI GCTCC 1 cut(s) 480
Lsp1109I GCAGC 1 cut(s) 267
MabI ACCWGGT 1 cut(s) 288
MalI GATC 3 cut(s) 8, 46, 252
MboI GATC 3 cut(s) 6, 44, 250
MboII GAAGA 3 cut(s) 16, 121, 203
MflI RGATCY 1 cut(s) 44
MhlI GDGCHC 1 cut(s) 477
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 1 cut(s) 314
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 7 cut(s) 35, 82, 91, 96, 171, 324, 370
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 2 cut(s) 452, 468
MslI CAYNNNNRTG 1 cut(s) 454
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 4 cut(s) 38, 53, 165, 260
MspR9I CCNGG 5 cut(s) 38, 53, 165, 290, 395
MvaI CCWGG 2 cut(s) 290, 395
NciI CCSGG 3 cut(s) 38, 53, 165
NdeII GATC 3 cut(s) 6, 44, 250
NlaIII CATG 1 cut(s) 209
NlaIV GGNNCC 1 cut(s) 46
NspI RCATGY 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 4 cut(s) 323, 356, 390, 439
PflFI GACNNNGTC 1 cut(s) 53
PkrI GCNGC 2 cut(s) 140, 282
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 477
Psp6I CCWGG 2 cut(s) 288, 393
PspGI CCWGG 2 cut(s) 288, 393
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 3 cut(s) 22, 105, 363
PsuI RGATCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 53
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
RseI CAYNNNNRTG 1 cut(s) 454
SacI GAGCTC 1 cut(s) 477
SaqAI TTAA 2 cut(s) 452, 468
SatI GCNGC 2 cut(s) 139, 281
Sau3AI GATC 3 cut(s) 6, 44, 250
Sau96I GGNCC 3 cut(s) 22, 105, 363
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 5 cut(s) 38, 53, 165, 290, 395
SduI GDGCHC 1 cut(s) 477
SetI ASST 7 cut(s) 83, 107, 294, 372, 399, 437, 477
SexAI ACCWGGT 1 cut(s) 288
SinI GGWCC 2 cut(s) 22, 105
SmiMI CAYNNNNRTG 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 1 cut(s) 314
SsiI CCGC 1 cut(s) 139
SstI GAGCTC 1 cut(s) 477
StyD4I CCNGG 5 cut(s) 36, 51, 163, 288, 393
TaaI ACNGT 1 cut(s) 145
TasI AATT 1 cut(s) 314
TatI WGTACW 1 cut(s) 202
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 4 cut(s) 323, 356, 390, 439
Tru1I TTAA 2 cut(s) 452, 468
Tru9I TTAA 2 cut(s) 452, 468
TseI GCWGC 1 cut(s) 280
TspDTI ATGAA 4 cut(s) 17, 112, 315, 476
TspGWI ACGGA 1 cut(s) 169
Tth111I GACNNNGTC 1 cut(s) 53
VpaK11BI GGWCC 2 cut(s) 22, 105
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 1 cut(s) 314
XceI RCATGY 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.