Rorug01G0465200

Belongs to the synaptobrevin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0005638
Physical Location & Seq
Forward (+)
55781471 .. 55791498
10028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0465200.1

Sequence Viewer

Length: 6630 bp
ATGTTGGCGGCGAGTTCCGGCGGTGGTGGTTCCGTTCTCCAACTCCGGACCAAACCTTCCGTCCTCGCTTCACCTCAGCTCAATGCCTCGCCGATAGCGAGGCTAAGCACCAGAGCCACTGCTCGCTCTGGTTCCAAAGCTATTGCGAAGAAGTTCTTCGGGACGAGATTGAGGGCCGCCGGATCCGACAGGCTGCACTTATGGCGATCCGACGGTCCCGGCCGCTCGCCGAAGCTGCGGGTGGTGGTCCGGAACATGCTCTCCGCCGTGCCGGAGAAGCCGCTCGGGTTATACGACCCGGCGATGGATAAGGACTCGTGCGGTGTCGGCTTCGTGGCGGAATTGTCCGGTGAAAGCAGCCGTAAAACGATTACGGATGCTCTGGAAATGTTGGTGCGGATGACACATAGAGGAGCTTGTGGATGTGAAACAAACACTGGTGATGGAGCTGGTGTTCTTGTGGCGATTCCTCACGATTTCTACAAGGAGGCTGCCAAAGATAATGGGTTTGAGCTTCCACCGCTAGGTGAGTATGCTGTTGGTATGCTCTACTTGCCTACATCGGAAAGTCGTAGAGAGGAAAGCAAAAATGTGTTTACAAAGGTTGCCGAGTCTCTTGGCCATACTGTTCTTGGGTGGCGATCAGTTCCCACTGATAACTCAGATTTGGGGAATTCTGCTCTCCAGACAGAACCTGTTATTGAGCAAGTGTTCCTTACTCCAACTCCGAGGTCGAAAGTTGATCTTGAGCGCCAGATGTACATACTTAGAAGGGTTTCAATGGTTGCCATCCGTGCTGCATTAAACCTTCAACATGGTGGTGCTAAAGATTTCTACATATGTTCACTTTCCTCGAGGACGATTGTTTACAAGGGTCAGCTGAAACCCGAACAGCTGAAGGGTTACTATTATGCAGATCTTGGCAATGAAAGGTTTACGAGCTACATGGCCCTGGTACATTCTCGATTCTCAACAAATACTTTCCCCAGCTGGGACCGTGCTCAGCCCATGCGTGTCATTGGCCACAATGGAGAAATCAACACCCTTAGAGGAAATGTTAACTGGATGAAGGCACGTGAGGGACTGCTTAAGTGTACTGAACTTGGTTTATCAAAAAATGAGTTAAAGAAGCTGTTACCAATTGTGGATGCCAGTTCATCCGATTCAGGAGCTTTTGATGGTGTCCTTGAGCTTCTAGTTCGAGCTGGCAGAAGCCTCCCTGAAGCTATTATGATGATGATTCCTGAAGCATGGCAAAATGACAAGAACATGGATCCTGATAGGAGAGCACTGTATGAATATTTCTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTAATATCATTTACTGATGGACGCTATCTGGGAGCTACATTGGATCGGAATGGGCTCCGACCGGGTCGCTTTTACGTCACCCACAGTGGACGAGTTATTATGGCCAGTGAAGTTGGGGTTGTAGACGTACCTCCAGAAGATGTCTGCAGGAAAGGAAGACTTAACCCTGGAATGATGCTTTTGGTGGATTTTGAGAATCATATAGTGGTCGATGATGAAGCCTTGAAGCAGCAATATTCACTGGCAAGGCCGTATGGGGAGTGGCTTAAAAGGCAGAAGATTGAGCTCAAGGACATAGTTGATTCTGTTCATGAATCTGACAGGGTCCCTCCATCCATAGCTGGAGTTGTTCCTGCATCTACTGATGATGACAACATGGAAAACATGGGAGTCCATGGTTTACTGGCACCACTGAAGGCTTTTGGTTACACAGTTGAAGCCTTGGAAATGTTGTTGCTTCCTATGGCAATAGATGGCGTAGAGGCTCTTGGGTCAATGGGAAATGATACCCCGTTGGCTGTTATGTCCAATAGAGAAAAGCTTACATTTGAGTATTTCAAGCAAATGTTCGCCCAAGTAACAAACCCACCAATTGATCCAATTCGAGAAAAGGTAGTCACGTCTATGGAATGTATGATCGGTCCAGAAGGTGATCTAACAGAAACCACTGAAGAACAATGCCGTCGTCTCTCACTCAAAGGTCCTCTTTTAAGGATTGAGGAAATGGAGGCGATCAAAAAAATGAATTACAGAGGTTGGCGCTGTAAGGTTCTTGACATAACTTACTCCAAGGAACATGGTAGAAAGGGATTGGAGGAGACCTTGGATAGGATCTGTGCTGAAGCACGTGAGGCAATTAAAAAGGGATATACCACACTGGTGCTTTCAGACAGAGCCTTCTCCCCAAAACGTGTTGCTGTAAGCTCACTCTTGGCTGTTGGTGCTGTTCATCAACATCTAGTTAAAAATCTTGAGCGCACGCGAGTAGGGTTGATAATTGAATCTGCTGAGCCGCGTGAGGTACACCATTTCTGTACGTTAGTTGGATTCGGTGCGGATGCCATCTGCCCCTACCTGGCTGTAGAGGCTATTTGGAGACTGCAGGTTGATGGAAAGATCCCACCAAAATCTAATGGTACAATATATTCCAAGGATGAACTGGTTAAAAAGTACTTCAAAGCTAGCAACTATGGAATGCAGAAGGTTCTCGCCAAGATGGGGATATCTACTTTGGCCTCTTACAAGGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCATCTGAAGTGATTGAGAGGTGCTTTGCAGGAACTCCTAGTAGAGTTGAGGGGGCAACATTTGAGATGCTTGCTCGTGATGGACTTCATCTGCATGATTTGGCGTTTCCCTCTCGGGCTTTTCCTCCGGGTAGTGCAGAAGCTGTGGCACTACCAAATCCAGGGGATTATCATTGGAGAAAAGGTGGTGAAGTTCACCTAAATGATCCTTTTGCTATTTCCAAGCTTCAGGAGGCTGCCAGAACTAACAGTGTGGCGGCATATAAAGAATACTCCAAGCTCATTCATCAATTAAATAAAGCCTGCAATCTCCGTGGTCTTCTGAAATTTAAAAACACAGAGCAGCAAATTCACTTGGATGAAGTGGAACCTGCCAGTGAGATTGTTAAACGGTTCTGTACTGGGGCAATGAGTTATGGATCAATATCATTGGAGGCACACACTACACTGGCCATTGCTATGAACAGAATGGGAGGAAAGTCGAATACAGGTGAGGGAGGTGAGCAGCCCTCTCGTATGGAGCCGCTTCCTGATGGTTCAAGGAATCCAAAGAGGAGCGCAATTAAGCAGGTTGCAAGTGGGAGATTTGGAGTTTCAAGTTACTATCTTACAAATGCTGATGAACTCCAAATAAAAATGGCTCAGGGGGCCAAGCCCGGTGAAGGAGGTGAGCTTCCTGGTCACAAGGTTATTGGAGACATTGCTGTTACCAGGAATTCCACTGCTGGTGTGGGACTTATCAGCCCACCTCCCCATCATGATATCTACTCAATTGAAGACCTTGCTCAATTAATTCATGATCTTAAGAATGCTAACCCAGGGGCTCGAATCAGCGTCAAGTTAGTGTCTGAAGCTGGTGTAGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCCGACCATGTTTTAATTGCCGGACATGATGGAGGCACTGGGGCCTCCAGATGGACCGGCATTAAGAATGCTGGGCTTCCATGGGAACTTGGCTTGGCTGAGACCCATCAAACCCTGGTTGCTAATGATCTTCGTGGCCGCACAGTTCTCCAGACAGATGGCCAACTAAAAACTGGAAGAGATGTGGCCATAGCCGCACTTCTTGGTGCAGAAGAGTTTGGGTTCAGCACGGCACCCCTCATAACCCTTGGCTGCATTATGATGCGGAAGTGCCACAAGAACACCTGTCCTGTCGGAATTGCAACCCAAGATCCAGTACTTCGTGAGAAGTTTGCCGGAGAACCTGAACATGTTATCAACTTTTTCTTCATGGTAGCTGAGGAGGTGAGAGAGATAATGTCACAACTTGGTTTTCGAACTTTAAATGAAATGGTTGGTCGTTCAGATATGCTTGAAGTCGACAAAGAAGTTACTAAAGGCAATGAGAAGCTGGACAACATTGATCTTTCCCTGTTACTTAGACCTGCTGCAGACATTCGTCCAGAAGCTTCACAATATTGTGTTCAGAAACAGGACCATGGGTTGGACATGGCTCTTGACCACAAACTTATTTCGCTGTCCAGTTCTGCTATAGAGAAGGCTCTTCCTGCATACTTTGAAACACCAATTTGCAATGTGAATCGTGCTGTTGGAACAATGCTTAGTCATGAGGTGACCAAACGTTACAACAGGGAAGGGCTTCCAGCAGACACCATTCATATCAAATTCAACGGAAGTGCAGGCCAGAGCCTTGGAGCTTTCCTCTGCCCTGGCATCACGCTTGAGCTTGAAGGTGACAGCAATGATTACGTTGGTAAAGGATTGTCTGGTGGCAAGATCATAGTTTATCCCCCCAAGGGAAGCAAGTTTGACCCAAAAGAAAACATTGTCATTGGTAATGTAGCTCTTTATGGTGCCACTAGTGGGGAGGCATATTTCAATGGAATGGCAGCAGAAAGATTCTGTGTTCGCAATTCAGGGGCAAGGGCAGTAGTTGAAGGTGTCGGTGATCATGGCTGTGAGTACATGACAGGTGGCACTGTTGTTGTGCTTGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGCATAGCCTACGTTCTTGATGTGGATGGAAAATTTTTGTCCCGATGCAATCCTGAGCTTGTAGATCTTGATAAAGTTGAAGGAGAAGACAGTATGACTCTTAAAATGATGATACAGCAGCATCAGCGTCATACAAAAAGCTTGCTGGCCAGTGAAGTACTGGCTGATTTTGAGAATCTTTTGCCTAAATTCATCAAGGTTATCCCCAGAGAGTACAAGCGTGCACTTGCAAACCTGAGGGAGGAGGCCTCCAAACAGACAGTTGATCAAGCTGATGAAGAAGCTCAGGAGCAAGAGGAAGAGGTGGAAATAAAGAAGAAAGATGCATTTGAAGAACTTAAGAAGATGGCATCTGCATCTTTAAATGGGACATCTAATCAGGTAGAAGATGCTGAAACATTGAAGAGGCCTAGTGAGGTCAGCAAAGCTGTTAAACACCGGGGTTTCATTTCTTATGAGCGTGAGGGCGTTCAATACAGGGATCCCAAAGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGTCCACTTGTGAATACGCAGTCAGCGCGTTGCATGGACTGTGGCACTCCTTTCTGCCATCAGGAAAACACTGGGTGTCCTCTTGGTAACAAAATACCTGAGTTCAATGAATTGGTGTACCAGAATAGGTGGCATGAAGCATTAGAGAGACTCCTCGAGACAAATAACTTCCCTGAGTTTACTGGTCGAGTTTGCCCAGCACCTTGTGAAGGTTCATGTGTTCTGGGTATTATTGAGAATCCTGTCTCTATTAAAAGCATTGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGTTGGATGGTGCCACGTCCTCCACTCAAGAGAACTGGGAAAAAGGTTGCCATTGTCGGAAGTGGGCCTGCTGGGCTAGCTGCTGCTGATCAACTAAACAGAATAGGTCATACGGTGACAGTTTACGAGCGTGCTGATAGAATTGGAGGGCTGATGATGTATGGAGTGCCTAATATGAAGGCTGACAAAGTTGATATAGTTCAACGGCGGGTTAACCTTATGGCCGAGGAAGGTGTCAACTTTGTGGTCAATGCTAATGTTGGAAATGATCCTTCCTACTCGTTTGATTGCCTTCGGGAGGAGAACAATGCGATTATTTTAGCAGTAGGCGCAACGAAGCCAAGGGACCTTCCCGTACCTGGGCGAGAGCTGTCAGGAGTACATTTTGCTATGGAGTTTCTTCATGCAAACACCAAAAGCTTGCTTGATAGCAATCTCAAGAATGGTAACTACATTTCTGCCAAGGGTAAGAAGGTTGTGGTCATTGGTGGAGGTGACACTGGCACTGATTGTATTGGAACCTCTGTTCGCCATGGCTGTACTAATGTCATAAATTTGGAGCTTCTCCCTGAGCCACCACGAACAAGGGCACCAGGAAACCCTTGGCCACAGTGGCCTCGTATATTCCGAGTAGATTATGGGCATGCAGAAGTTGCAGCCAAGTTTGGCAAAGATCCAAGAACTTATGAGGTGCTGACTAAGAGGTTTGTGGGAGATGAGAATGGGGTTGTGAAGGGACTTGAGGTAGTACGTGTCAAATGGGAGAAGGATGCTACTGGCAAGTTCCAATTTAAGGAAATCGAGGGCTCTGAGGAGATAATTGAGACCGATCTTGTCCTTCTAGCAATGGGGTTCCTTGGACCTGAGGCGGTGATTGCAGAGAAGTTGGGCTTGGAGCGTGACAATCGCTCAAACTTCAAGGCAGATTATGGTCGGTTCTCAACCAATGTGGATGGGGTCTTTGCTGCTGGGGATTGCCGGCGTGGTCAATCCCTGGTGGTGTGGGCAATCTCAGAGGGACGGCAAGCAGCTGCACAGGTTGACAACTACCTCTGCAAGGAAGAAGAAGACCACACAAACAGCAACGGGAGCCCTGAATCCGATCTATTGAAGAGGCATCAAGAAATCAGCAAGAGCCATAAGACAGTTGCAAGCACACCATAG

Protein Analysis

2209

Amino Acids

242.1

Weight (kDa)

6.34

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase_2 PF00310 107 - 534 4.5e-184 Glutamine amidotransferases class-II
Glu_syn_central PF04898 584 - 872 2.3e-111 Glutamate synthase central domain
Glu_synthase PF01645 939 - 1307 5.3e-150 Conserved region in glutamate synthase
GXGXG PF01493 1391 - 1576 7.6e-89 GXGXG motif
Fer4_20 PF14691 1712 - 1823 6.7e-22 Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2 PF07992 1836 - 2162 1.4e-26 Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8 PF13450 1840 - 1875 1.9e-08 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 853
Acc36I ACCTGC 4 cut(s) 2431, 2992, 3172, 4039
AccB1I GGYRCC 5 cut(s) 1744, 3739, 4460, 5476, 6055
AccB7I CCANNNNNTGG 2 cut(s) 1328, 4090
AccBSI CCGCTC 2 cut(s) 225, 283
AccI GTMKAC 2 cut(s) 1461, 3966
AccII CGCG 3 cut(s) 2320, 2353, 5200
AccIII TCCGGA 2 cut(s) 45, 249
AclI AACGTT 1 cut(s) 4228
AcsI RAATTY 8 cut(s) 673, 2939, 2961, 3328, 4271, 4670, 4826, 6019
AcuI CTGAAG 9 cut(s) 917, 1242, 1266, 1772, 2028, 2199, 2640, 2825, 3483
AcvI CACGTG 2 cut(s) 1076, 2186
AdeI CACNNNGTG 2 cut(s) 5247, 5378
AflII CTTAAG 3 cut(s) 1088, 3416, 4976
AflIII ACRYGT 3 cut(s) 2248, 3856, 6217
AhlI ACTAGT 1 cut(s) 4466
AjiI CACGTC 2 cut(s) 1959, 5483
AjuI GAANNNNNNNTTGG 2 cut(s) 6341, 6373
AleI CACNNNNGTG 1 cut(s) 2216
AloI GAACNNNNNNTCC 6 cut(s) 245, 277, 438, 470, 5976, 6008
Alw21I GWGCWC 5 cut(s) 1003, 1291, 1626, 2590, 4864
Alw44I GTGCAC 2 cut(s) 2586, 4860
AlwNI CAGNNNCTG 2 cut(s) 695, 2756
Ama87I CYCGRG 4 cut(s) 284, 853, 2727, 5327
Aor13HI TCCGGA 2 cut(s) 45, 249
ApaLI GTGCAC 2 cut(s) 2586, 4860
ApoI RAATTY 8 cut(s) 673, 2939, 2961, 3328, 4271, 4670, 4826, 6019
ArsI GACNNNNNNTTYG 4 cut(s) 45, 77, 5441, 5473
AseI ATTAAT 1 cut(s) 3404
Asp700I GAANNNNTTC 4 cut(s) 152, 155, 775, 4245
AspLEI GCGC 6 cut(s) 753, 2100, 2316, 3173, 5200, 5798
AsuC2I CCSGG 6 cut(s) 219, 299, 1401, 2742, 3270, 5078
AsuII TTCGAA 1 cut(s) 3922
AsuNHI GCTAGC 2 cut(s) 2519, 5542
AvaI CYCGRG 4 cut(s) 284, 853, 2727, 5327
AxyI CCTNAGG 2 cut(s) 4874, 6330
BaeGI GKGCMC 3 cut(s) 2590, 4864, 6058
BalI TGGCCA 8 cut(s) 621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
BamHI GGATCC 3 cut(s) 182, 1273, 5119
BanI GGYRCC 5 cut(s) 1744, 3739, 4460, 5476, 6055
BanII GRGCYC 5 cut(s) 1395, 1626, 3439, 6275, 6560
BauI CACGAG 2 cut(s) 316, 2688
BbrPI CACGTG 2 cut(s) 1076, 2186
BbsI GAAGAC 5 cut(s) 1501, 2924, 3396, 4731, 6540
Bbv12I GWGCWC 5 cut(s) 1003, 1291, 1626, 2590, 4864
BbvCI CCTCAGC 2 cut(s) 75, 3885
BceAI ACGGC 7 cut(s) 251, 345, 1573, 2004, 3753, 5687, 6503
BcgI CGANNNNNNTGC 2 cut(s) 3107, 3141
BclI TGATCA 3 cut(s) 4555, 4903, 5554
BcnI CCSGG 6 cut(s) 219, 299, 1401, 2742, 3270, 5078
BcuI ACTAGT 1 cut(s) 4466
BfmI CTRYAG 5 cut(s) 1483, 2418, 2438, 4035, 4137
BfoI RGCGCY 2 cut(s) 754, 2101
BfrI CTTAAG 3 cut(s) 1088, 3416, 4976
BfuAI ACCTGC 4 cut(s) 2431, 2992, 3172, 4039
BglI GCCNNNNNGGC 2 cut(s) 5539, 6079
BglII AGATCT 2 cut(s) 916, 4702
BlpI GCTNAGC 3 cut(s) 104, 1002, 2346
BmcAI AGTACT 3 cut(s) 2510, 3825, 4797
BmeT110I CYCGRG 4 cut(s) 284, 853, 2727, 5327
BmgBI CACGTC 2 cut(s) 1959, 5483
BmrI ACTGGG 4 cut(s) 3024, 3555, 5253, 5511
BmtI GCTAGC 2 cut(s) 2523, 5546
BmuI ACTGGG 4 cut(s) 3024, 3555, 5253, 5511
BoxI GACNNNNGTC 1 cut(s) 4044
BpiI GAAGAC 5 cut(s) 1501, 2924, 3396, 4731, 6540
BplI GAGNNNNNCTC 6 cut(s) 3107, 3139, 4293, 4325, 4871, 4903
BpmI CTGGAG 5 cut(s) 668, 1455, 1701, 3538, 3641
Bpu10I CCTNAGC 6 cut(s) 75, 3255, 3885, 4692, 4923, 6036
Bpu1102I GCTNAGC 3 cut(s) 104, 1002, 2346
Bpu14I TTCGAA 1 cut(s) 3922
BpuEI CTTGAG 8 cut(s) 767, 1208, 1610, 2330, 4349, 5477, 5888, 6227
BpuMI CCSGG 6 cut(s) 219, 299, 1401, 2742, 3270, 5078
BsaAI YACGTR 3 cut(s) 1076, 2186, 6218
BsaBI GATNNNNATC 2 cut(s) 3037, 5898
BsaI GGTCTC 3 cut(s) 2150, 3602, 6284
BsaWI WCCGGW 3 cut(s) 45, 249, 347
BsaXI ACNNNNNCTCC 8 cut(s) 245, 275, 438, 468, 709, 739, 2782, 2812
Bse118I RCCGGY 2 cut(s) 3563, 6444
Bse21I CCTNAGG 2 cut(s) 4874, 6330
Bse3DI GCAATG 8 cut(s) 931, 3027, 3066, 3312, 3994, 4186, 4354, 6318
Bse8I GATNNNNATC 2 cut(s) 3037, 5898
BseAI TCCGGA 2 cut(s) 45, 249
BseJI GATNNNNATC 2 cut(s) 3037, 5898
BseMI GCAATG 8 cut(s) 931, 3027, 3066, 3312, 3994, 4186, 4354, 6318
BseRI GAGGAG 8 cut(s) 426, 2168, 3181, 3902, 4895, 5315, 5780, 6293
BseSI GKGCMC 3 cut(s) 2590, 4864, 6058
BseX3I CGGCCG 1 cut(s) 220
BseYI CCCAGC 6 cut(s) 986, 990, 3578, 5368, 5537, 6434
BsgI GTGCAG 5 cut(s) 179, 2769, 3735, 4305, 6483
Bsh1236I CGCG 3 cut(s) 2320, 2353, 5200
Bsh1285I CGRYCG 2 cut(s) 223, 1400
BshNI GGYRCC 5 cut(s) 1744, 3739, 4460, 5476, 6055
BsiEI CGRYCG 2 cut(s) 223, 1400
BsiHKAI GWGCWC 5 cut(s) 1003, 1291, 1626, 2590, 4864
BsiHKCI CYCGRG 4 cut(s) 284, 853, 2727, 5327
BsmBI CGTCTC 1 cut(s) 2030
BsmI GAATGC 3 cut(s) 2538, 3427, 3580
Bso31I GGTCTC 3 cut(s) 2150, 3602, 6284
BsoBI CYCGRG 4 cut(s) 284, 853, 2727, 5327
Bsp119I TTCGAA 1 cut(s) 3922
Bsp13I TCCGGA 2 cut(s) 45, 249
Bsp1407I TGTACA 1 cut(s) 759
Bsp1720I GCTNAGC 3 cut(s) 104, 1002, 2346
Bsp19I CCATGG 5 cut(s) 1322, 1732, 3587, 4084, 5998
BspEI TCCGGA 2 cut(s) 45, 249
BspFNI CGCG 3 cut(s) 2320, 2353, 5200
BspHI TCATGA 4 cut(s) 1648, 3370, 3409, 4213
BspMAI CTGCAG 3 cut(s) 1487, 2442, 4039
BspMI ACCTGC 4 cut(s) 2431, 2992, 3172, 4039
BspOI GCTAGC 2 cut(s) 2523, 5546
BspQI GCTCTTC 1 cut(s) 4155
BspT104I TTCGAA 1 cut(s) 3922
BspT107I GGYRCC 5 cut(s) 1744, 3739, 4460, 5476, 6055
BspTI CTTAAG 3 cut(s) 1088, 3416, 4976
BspTNI GGTCTC 3 cut(s) 2150, 3602, 6284
BsrBI CCGCTC 2 cut(s) 225, 283
BsrDI GCAATG 8 cut(s) 931, 3027, 3066, 3312, 3994, 4186, 4354, 6318
BsrFI RCCGGY 2 cut(s) 3563, 6444
BsrGI TGTACA 1 cut(s) 759
BssAI RCCGGY 2 cut(s) 3563, 6444
BssSI CACGAG 2 cut(s) 316, 2688
Bst2BI CACGAG 2 cut(s) 316, 2688
Bst6I CTCTTC 6 cut(s) 3679, 3714, 4155, 4932, 5036, 6572
BstAFI CTTAAG 3 cut(s) 1088, 3416, 4976
BstAPI GCANNNNNTGC 1 cut(s) 6119
BstAUI TGTACA 1 cut(s) 759
BstBAI YACGTR 3 cut(s) 1076, 2186, 6218
BstBI TTCGAA 1 cut(s) 3922
BstDSI CCRYGG 6 cut(s) 1322, 1732, 2926, 3587, 4084, 5998
BstEII GGTNACC 1 cut(s) 4219
BstENI CCTNNNNNAGG 5 cut(s) 2164, 4877, 5379, 5762, 6521
BstFNI CGCG 3 cut(s) 2320, 2353, 5200
BstH2I RGCGCY 2 cut(s) 754, 2101
BstHHI GCGC 6 cut(s) 753, 2100, 2316, 3173, 5200, 5798
BstMCI CGRYCG 2 cut(s) 223, 1400
BstNSI RCATGY 4 cut(s) 259, 3509, 3860, 6113
BstPAI GACNNNNGTC 1 cut(s) 4044
BstPI GGTNACC 1 cut(s) 4219
BstSFI CTRYAG 5 cut(s) 1483, 2418, 2438, 4035, 4137
BstSLI GKGCMC 3 cut(s) 2590, 4864, 6058
BstUI CGCG 3 cut(s) 2320, 2353, 5200
BstV2I GAAGAC 5 cut(s) 1501, 2924, 3396, 4731, 6540
BstX2I RGATCY 9 cut(s) 182, 916, 1273, 2169, 2454, 3817, 4702, 5119, 6139
BstXI CCANNNNNNTGG 2 cut(s) 3665, 4298
BstYI RGATCY 9 cut(s) 182, 916, 1273, 2169, 2454, 3817, 4702, 5119, 6139
BstZI CGGCCG 1 cut(s) 220
Bsu36I CCTNAGG 2 cut(s) 4874, 6330
BtgI CCRYGG 6 cut(s) 1322, 1732, 2926, 3587, 4084, 5998
BtgZI GCGATG 1 cut(s) 317
BtrI CACGTC 2 cut(s) 1959, 5483
BtsI GCAGTG 2 cut(s) 117, 3333
BveI ACCTGC 4 cut(s) 2431, 2992, 3172, 4039
CaiI CAGNNNCTG 2 cut(s) 695, 2756
CciI TCATGA 4 cut(s) 1648, 3370, 3409, 4213
CfoI GCGC 6 cut(s) 753, 2100, 2316, 3173, 5200, 5798
Cfr10I RCCGGY 2 cut(s) 3563, 6444
CseI GACGC 3 cut(s) 1368, 3436, 4754
CsiI ACCWGGT 1 cut(s) 5170
CspCI CAANNNNNGTGG 4 cut(s) 2908, 2943, 6396, 6431
DraI TTTAAA 3 cut(s) 2944, 3930, 5001
DraIII CACNNNGTG 2 cut(s) 5247, 5378
EagI CGGCCG 1 cut(s) 220
Eam1104I CTCTTC 6 cut(s) 3679, 3714, 4155, 4932, 5036, 6572
EarI CTCTTC 6 cut(s) 3679, 3714, 4155, 4932, 5036, 6572
EciI GGCGGA 2 cut(s) 253, 353
Ecl136II GAGCTC 1 cut(s) 1624
EclXI CGGCCG 1 cut(s) 220
Eco147I AGGCCT 2 cut(s) 4886, 5047
Eco24I GRGCYC 5 cut(s) 1395, 1626, 3439, 6275, 6560
Eco31I GGTCTC 3 cut(s) 2150, 3602, 6284
Eco32I GATATC 2 cut(s) 2562, 3376
Eco52I CGGCCG 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 1624
Eco57I CTGAAG 9 cut(s) 917, 1242, 1266, 1772, 2028, 2199, 2640, 2825, 3483
Eco72I CACGTG 2 cut(s) 1076, 2186
Eco81I CCTNAGG 2 cut(s) 4874, 6330
Eco88I CYCGRG 4 cut(s) 284, 853, 2727, 5327
Eco91I GGTNACC 1 cut(s) 4219
EcoICRI GAGCTC 1 cut(s) 1624
EcoNI CCTNNNNNAGG 5 cut(s) 2164, 4877, 5379, 5762, 6521
EcoO109I RGGNCCY 4 cut(s) 1663, 2039, 3549, 5812
EcoO65I GGTNACC 1 cut(s) 4219
EcoRI GAATTC 2 cut(s) 673, 3328
EcoRV GATATC 2 cut(s) 2562, 3376
EcoT22I ATGCAT 1 cut(s) 4966
EcoT38I GRGCYC 5 cut(s) 1395, 1626, 3439, 6275, 6560
Esp3I CGTCTC 1 cut(s) 2030
FauI CCCGC 2 cut(s) 231, 5667
FauNDI CATATG 1 cut(s) 839
FbaI TGATCA 3 cut(s) 4555, 4903, 5554
FblI GTMKAC 2 cut(s) 1461, 3966
FriOI GRGCYC 5 cut(s) 1395, 1626, 3439, 6275, 6560
GlaI GCGC 6 cut(s) 752, 2099, 2315, 3172, 5199, 5797
GsaI CCCAGC 6 cut(s) 990, 994, 3582, 5372, 5541, 6438
GsuI CTGGAG 5 cut(s) 668, 1455, 1701, 3538, 3641
HaeII RGCGCY 2 cut(s) 754, 2101
HgaI GACGC 3 cut(s) 1368, 3436, 4754
HhaI GCGC 6 cut(s) 753, 2100, 2316, 3173, 5200, 5798
Hin6I GCGC 6 cut(s) 751, 2098, 2314, 3171, 5198, 5796
HinP1I GCGC 6 cut(s) 751, 2098, 2314, 3171, 5198, 5796
HincII GTYRAC 5 cut(s) 1060, 3967, 5680, 5704, 6508
HindII GTYRAC 5 cut(s) 1060, 3967, 5680, 5704, 6508
HindIII AAGCTT 6 cut(s) 1877, 2601, 2837, 4053, 4777, 5884
HpaI GTTAAC 2 cut(s) 1060, 5680
Hpy99I CGWCG 2 cut(s) 215, 2025
HspAI GCGC 6 cut(s) 751, 2098, 2314, 3171, 5198, 5796
KflI GGGWCCC 1 cut(s) 1663
Kpn2I TCCGGA 2 cut(s) 45, 249
KroI GCCGGC 1 cut(s) 6444
KroNI GCCGGC 1 cut(s) 6446
Ksp22I TGATCA 3 cut(s) 4555, 4903, 5554
KspAI GTTAAC 2 cut(s) 1060, 5680
LguI GCTCTTC 1 cut(s) 4155
MabI ACCWGGT 1 cut(s) 5170
MbiI CCGCTC 2 cut(s) 225, 283
MfeI CAATTG 3 cut(s) 1140, 1929, 3384
MflI RGATCY 9 cut(s) 182, 916, 1273, 2169, 2454, 3817, 4702, 5119, 6139
MlsI TGGCCA 8 cut(s) 621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MluNI TGGCCA 8 cut(s) 621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MlyI GAGTC 5 cut(s) 308, 620, 1737, 4729, 5316
Mox20I TGGCCA 8 cut(s) 621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
Mph1103I ATGCAT 1 cut(s) 4966
MroI TCCGGA 2 cut(s) 45, 249
MroNI GCCGGC 1 cut(s) 6444
MroXI GAANNNNTTC 4 cut(s) 152, 155, 775, 4245
MscI TGGCCA 8 cut(s) 621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MslI CAYNNNNRTG 9 cut(s) 819, 1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
Msp20I TGGCCA 8 cut(s) 621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MspA1I CMGCKG 4 cut(s) 880, 895, 990, 6497
MspCI CTTAAG 3 cut(s) 1088, 3416, 4976
MunI CAATTG 3 cut(s) 1140, 1929, 3384
Mva1269I GAATGC 3 cut(s) 2538, 3427, 3580
MvnI CGCG 3 cut(s) 2320, 2353, 5200
NaeI GCCGGC 1 cut(s) 6446
NciI CCSGG 6 cut(s) 219, 299, 1401, 2742, 3270, 5078
NcoI CCATGG 5 cut(s) 1322, 1732, 3587, 4084, 5998
NdeI CATATG 1 cut(s) 839
NgoMIV GCCGGC 1 cut(s) 6444
NheI GCTAGC 2 cut(s) 2519, 5542
NmeAIII GCCGAG 2 cut(s) 634, 5716
NmuCI GTSAC 9 cut(s) 1414, 1954, 3293, 3906, 4219, 4340, 5581, 5960, 6365
NsiI ATGCAT 1 cut(s) 4966
NspI RCATGY 4 cut(s) 259, 3509, 3860, 6113
NspV TTCGAA 1 cut(s) 3922
OliI CACNNNNGTG 1 cut(s) 2216
PaeI GCATGC 2 cut(s) 3509, 6113
PaeR7I CTCGAG 2 cut(s) 853, 5327
PagI TCATGA 4 cut(s) 1648, 3370, 3409, 4213
PasI CCCWGGG 1 cut(s) 3431
PceI AGGCCT 2 cut(s) 4886, 5047
PciI ACATGT 1 cut(s) 3856
PciSI GCTCTTC 1 cut(s) 4155
PcsI WCGNNNNNNNCGW 3 cut(s) 95, 291, 6091
PctI GAATGC 3 cut(s) 2538, 3427, 3580
PdiI GCCGGC 1 cut(s) 6446
PdmI GAANNNNTTC 4 cut(s) 152, 155, 775, 4245
PflFI GACNNNGTC 2 cut(s) 1401, 1661
PflMI CCANNNNNTGG 2 cut(s) 1328, 4090
PleI GAGTC 5 cut(s) 308, 619, 1736, 4729, 5316
PmaCI CACGTG 2 cut(s) 1076, 2186
PmlI CACGTG 2 cut(s) 1076, 2186
PpsI GAGTC 5 cut(s) 308, 619, 1736, 4729, 5316
Ppu21I YACGTR 3 cut(s) 1076, 2186, 6218
PpuMI RGGWCCY 3 cut(s) 1663, 2039, 5812
PscI ACATGT 1 cut(s) 3856
PshAI GACNNNNGTC 1 cut(s) 4044
PshBI ATTAAT 1 cut(s) 3404
Psp124BI GAGCTC 1 cut(s) 1626
Psp1406I AACGTT 1 cut(s) 4228
Psp5II RGGWCCY 3 cut(s) 1663, 2039, 5812
PspCI CACGTG 2 cut(s) 1076, 2186
PspEI GGTNACC 1 cut(s) 4219
PspFI CCCAGC 6 cut(s) 986, 990, 3578, 5368, 5537, 6434
PspPPI RGGWCCY 3 cut(s) 1663, 2039, 5812
PspXI VCTCGAGB 1 cut(s) 853
PstI CTGCAG 3 cut(s) 1487, 2442, 4039
PstNI CAGNNNCTG 2 cut(s) 695, 2756
PsuI RGATCY 9 cut(s) 182, 916, 1273, 2169, 2454, 3817, 4702, 5119, 6139
PsyI GACNNNGTC 2 cut(s) 1401, 1661
PvuII CAGCTG 4 cut(s) 880, 895, 990, 6497
RseI CAYNNNNRTG 9 cut(s) 819, 1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
SacI GAGCTC 1 cut(s) 1626
SalI GTCGAC 1 cut(s) 3965
SapI GCTCTTC 1 cut(s) 4155
ScaI AGTACT 3 cut(s) 2510, 3825, 4797
SchI GAGTC 5 cut(s) 308, 620, 1737, 4729, 5316
SexAI ACCWGGT 1 cut(s) 5170
SfcI CTRYAG 5 cut(s) 1483, 2418, 2438, 4035, 4137
SfiI GGCCNNNNNGGCC 1 cut(s) 6079
Sfr274I CTCGAG 2 cut(s) 853, 5327
SfuI TTCGAA 1 cut(s) 3922
SlaI CTCGAG 2 cut(s) 853, 5327
SmiMI CAYNNNNRTG 9 cut(s) 819, 1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
SpeI ACTAGT 1 cut(s) 4466
SphI GCATGC 2 cut(s) 3509, 6113
SseBI AGGCCT 2 cut(s) 4886, 5047
SspI AATATT 3 cut(s) 1301, 1574, 4064
SstI GAGCTC 1 cut(s) 1626
StuI AGGCCT 2 cut(s) 4886, 5047
TaqII GACCGA 2 cut(s) 1967, 6308
TauI GCSGC 9 cut(s) 11, 179, 225, 283, 2353, 2873, 3139, 3648, 3704
TseFI GTSAC 9 cut(s) 1414, 1954, 3293, 3906, 4219, 4340, 5581, 5960, 6365
Tsp45I GTSAC 9 cut(s) 1414, 1954, 3293, 3906, 4219, 4340, 5581, 5960, 6365
TspGWI ACGGA 6 cut(s) 22, 49, 389, 782, 2915, 4293
Tth111I GACNNNGTC 2 cut(s) 1401, 1661
Van91I CCANNNNNTGG 2 cut(s) 1328, 4090
Vha464I CTTAAG 3 cut(s) 1088, 3416, 4976
VneI GTGCAC 2 cut(s) 2586, 4860
VspI ATTAAT 1 cut(s) 3404
XagI CCTNNNNNAGG 5 cut(s) 2164, 4877, 5379, 5762, 6521
XapI RAATTY 8 cut(s) 673, 2939, 2961, 3328, 4271, 4670, 4826, 6019
XceI RCATGY 4 cut(s) 259, 3509, 3860, 6113
XcmI CCANNNNNNNNNTGG 6 cut(s) 629, 2494, 3340, 3677, 4795, 6066
XhoI CTCGAG 2 cut(s) 853, 5327
XmiI GTMKAC 2 cut(s) 1461, 3966
XmnI GAANNNNTTC 4 cut(s) 152, 155, 775, 4245
ZrmI AGTACT 3 cut(s) 2510, 3825, 4797
Zsp2I ATGCAT 1 cut(s) 4966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.