Rh2BG014900

Regulated-SNARE-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005638
Physical Location & Seq
Forward (+)
1105841 .. 1110963
5123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG014900.1

Sequence Viewer

Length: 492 bp
ATGAAGATCACGGCGTTGCTGGTCCTCAAGTGCAACCCGGAAGGATCCGACCCGGTCATATTGGCCAACGCCTCCGATACCTCCCACTTCGGCTACTTCCAGAGGTCCAGCGTCAAGGAGTTCATCGTCTTCGTCGGCCGCACCGTCGCCAAACGCACCCCTCCCGGTCAACGTCAGTCCGTCAAGCACGAAGAGTATAAAGTACATGCTTACAGCAGCAATGGACTCTGTGCATTGGGCTTTATGGATGATCACTATCCGGTTAGAAGTGCGTTTTCGCTGCTGAACCAGGTGTTAGATGAATATCAGAAAAAATTTGGTGATTCTTGGAGGACTGCACAACAGGACAGCACAGAATCGTGGCCCTACCTAAATGAGGCTCTAACAAGATTCCAGGACCCGGCAGAAGCTGATAAGTTGTTGAAAATTCAGAGGGAATTGGATGAAACAAAGATTATTCTTGTGAGTATTGTGCCTTCAGCTACATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 ko04138 map04130 map04138
Pfam Domains
Protein Families
Longin Synaptobrevin

Protein Analysis

163

Amino Acids

18.4

Weight (kDa)

6.42

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 47 - 112 1.4e-15 Regulated-SNARE-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 139
AclWI GGATC 2 cut(s) 39, 52
AcoI YGGCCR 2 cut(s) 63, 136
AcsI RAATTY 2 cut(s) 314, 426
AcuI CTGAAG 1 cut(s) 462
AfaI GTAC 1 cut(s) 204
AfiI CCNNNNNNNGG 2 cut(s) 376, 400
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 2 cut(s) 288, 393
AluBI AGCT 2 cut(s) 410, 482
AluI AGCT 2 cut(s) 410, 482
AlwI GGATC 2 cut(s) 39, 52
AlwNI CAGNNNCTG 1 cut(s) 410
AoxI GGCC 3 cut(s) 63, 136, 362
ApeKI GCWGC 2 cut(s) 216, 280
ApoI RAATTY 2 cut(s) 314, 426
AspS9I GGNCC 4 cut(s) 22, 105, 363, 397
AsuC2I CCSGG 4 cut(s) 38, 53, 165, 401
AsuHPI GGTGA 1 cut(s) 332
AvaII GGWCC 3 cut(s) 22, 105, 397
BalI TGGCCA 1 cut(s) 65
BamHI GGATCC 1 cut(s) 44
BarI GAAGNNNNNNTAC 2 cut(s) 460, 492
BbsI GAAGAC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 228, 267
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 290, 395
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 4 cut(s) 38, 53, 165, 401
BisI GCNGC 3 cut(s) 139, 217, 281
BlsI GCNGC 3 cut(s) 140, 218, 282
Bme1390I CCNGG 6 cut(s) 38, 53, 165, 290, 395, 401
Bme18I GGWCC 3 cut(s) 22, 105, 397
BmgT120I GGNCC 4 cut(s) 22, 105, 363, 397
BmiI GGNNCC 2 cut(s) 46, 399
BmrFI CCNGG 6 cut(s) 38, 53, 165, 290, 395, 401
BpiI GAAGAC 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 11
BpuMI CCSGG 4 cut(s) 38, 53, 165, 401
BsaBI GATNNNNATC 2 cut(s) 255, 303
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 400
Bse3DI GCAATG 1 cut(s) 226
Bse8I GATNNNNATC 2 cut(s) 255, 303
BseBI CCWGG 2 cut(s) 290, 395
BseGI GGATG 2 cut(s) 253, 448
BseJI GATNNNNATC 2 cut(s) 255, 303
BseLI CCNNNNNNNGG 2 cut(s) 376, 400
BseMI GCAATG 1 cut(s) 226
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 2 cut(s) 228, 267
BsgI GTGCAG 1 cut(s) 321
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 3 cut(s) 65, 138, 364
BsiEI CGRYCG 1 cut(s) 139
BsiSI CCGG 5 cut(s) 38, 53, 165, 260, 401
BslI CCNNNNNNNGG 2 cut(s) 376, 400
BsnI GGCC 3 cut(s) 65, 138, 364
Bsp143I GATC 3 cut(s) 6, 44, 250
BspACI CCGC 1 cut(s) 139
BspANI GGCC 3 cut(s) 65, 138, 364
BspLI GGNNCC 2 cut(s) 46, 399
BspPI GGATC 2 cut(s) 39, 52
BsrDI GCAATG 1 cut(s) 226
BssMI GATC 3 cut(s) 6, 44, 250
Bst2UI CCWGG 2 cut(s) 290, 395
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 186
BstENI CCTNNNNNAGG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 253, 448
BstKTI GATC 3 cut(s) 9, 47, 253
BstMBI GATC 3 cut(s) 6, 44, 250
BstMCI CGRYCG 1 cut(s) 139
BstNI CCWGG 2 cut(s) 290, 395
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 6 cut(s) 36, 51, 163, 288, 393, 399
BstV1I GCAGC 2 cut(s) 228, 267
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 44
BstYI RGATCY 1 cut(s) 44
BstZI CGGCCG 1 cut(s) 136
BsuRI GGCC 3 cut(s) 65, 138, 364
BtsCI GGATG 2 cut(s) 253, 448
CaiI CAGNNNCTG 1 cut(s) 410
Cfr13I GGNCC 4 cut(s) 22, 105, 363, 397
CseI GACGC 1 cut(s) 100
CsiI ACCWGGT 1 cut(s) 288
Csp6I GTAC 1 cut(s) 203
CviAII CATG 1 cut(s) 206
CviJI RGCY 8 cut(s) 65, 93, 138, 240, 364, 380, 410, 482
CviKI_1 RGCY 8 cut(s) 65, 93, 138, 240, 364, 380, 410, 482
CviQI GTAC 1 cut(s) 203
DpnI GATC 3 cut(s) 8, 46, 252
DpnII GATC 3 cut(s) 6, 44, 250
EaeI YGGCCR 2 cut(s) 63, 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EclXI CGGCCG 1 cut(s) 136
Eco47I GGWCC 3 cut(s) 22, 105, 397
Eco52I CGGCCG 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 462
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 288, 393
FaeI CATG 1 cut(s) 209
FaiI YATR 4 cut(s) 59, 198, 207, 245
FatI CATG 1 cut(s) 205
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 3 cut(s) 139, 217, 281
FokI GGATG 2 cut(s) 260, 455
Fsp4HI GCNGC 3 cut(s) 139, 217, 281
GluI GCNGC 3 cut(s) 139, 217, 281
HaeIII GGCC 3 cut(s) 65, 138, 364
HapII CCGG 5 cut(s) 38, 53, 165, 260, 401
HgaI GACGC 1 cut(s) 100
Hin1II CATG 1 cut(s) 209
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HinfI GANTC 4 cut(s) 225, 323, 356, 390
HpaII CCGG 5 cut(s) 38, 53, 165, 260, 401
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 4 cut(s) 49, 76, 309, 432
Hpy188III TCNNGA 2 cut(s) 100, 489
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 2 cut(s) 137, 149
HpyAV CCTTC 2 cut(s) 35, 486
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 3 cut(s) 33, 233, 338
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 3 cut(s) 6, 44, 250
Lsp1109I GCAGC 2 cut(s) 228, 267
MabI ACCWGGT 1 cut(s) 288
MaeII ACGT 1 cut(s) 172
MalI GATC 3 cut(s) 8, 46, 252
MboI GATC 3 cut(s) 6, 44, 250
MboII GAAGA 3 cut(s) 16, 121, 203
MflI RGATCY 1 cut(s) 44
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 3 cut(s) 314, 426, 437
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 8 cut(s) 35, 82, 91, 96, 171, 324, 370, 426
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 65
MspI CCGG 5 cut(s) 38, 53, 165, 260, 401
MspR9I CCNGG 6 cut(s) 38, 53, 165, 290, 395, 401
MvaI CCWGG 2 cut(s) 290, 395
NciI CCSGG 4 cut(s) 38, 53, 165, 401
NdeII GATC 3 cut(s) 6, 44, 250
NlaIII CATG 1 cut(s) 209
NlaIV GGNNCC 2 cut(s) 46, 399
NspI RCATGY 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PfeI GAWTC 3 cut(s) 323, 356, 390
PflFI GACNNNGTC 1 cut(s) 53
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 3 cut(s) 140, 218, 282
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 397
Psp5II RGGWCCY 1 cut(s) 397
Psp6I CCWGG 2 cut(s) 288, 393
PspGI CCWGG 2 cut(s) 288, 393
PspN4I GGNNCC 2 cut(s) 46, 399
PspPI GGNCC 4 cut(s) 22, 105, 363, 397
PspPPI RGGWCCY 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 53
RsaI GTAC 1 cut(s) 204
RsaNI GTAC 1 cut(s) 203
SatI GCNGC 3 cut(s) 139, 217, 281
Sau3AI GATC 3 cut(s) 6, 44, 250
Sau96I GGNCC 4 cut(s) 22, 105, 363, 397
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 6 cut(s) 38, 53, 165, 290, 395, 401
SetI ASST 7 cut(s) 83, 107, 175, 294, 372, 412, 484
SexAI ACCWGGT 1 cut(s) 288
SinI GGWCC 3 cut(s) 22, 105, 397
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 3 cut(s) 314, 426, 437
SsiI CCGC 1 cut(s) 139
StyD4I CCNGG 6 cut(s) 36, 51, 163, 288, 393, 399
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 175
TasI AATT 3 cut(s) 314, 426, 437
TatI WGTACW 1 cut(s) 202
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 3 cut(s) 323, 356, 390
TseI GCWGC 2 cut(s) 216, 280
TspDTI ATGAA 4 cut(s) 17, 112, 315, 459
TspGWI ACGGA 1 cut(s) 169
Tth111I GACNNNGTC 1 cut(s) 53
VpaK11BI GGWCC 3 cut(s) 22, 105, 397
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 2 cut(s) 314, 426
XceI RCATGY 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.