Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002065

Physical Location & Seq

Genomic Coordinates Forward (+)

8298001 .. 8306287

Gene Structure 8287 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1103900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5463 bp

ATGGGCACTCCCGACAACAAACTATCCGAGTTAGTTGACATATTCAGGTCTTGGATCCCCAGGAGGTCCGAGCCGCCCAATGTGTCCAGGGACTTTTGGATGCCTGATCAAAGCTGTAGGGTATGTTATGACTGTGACTCTCAATTCACAATATTTAACCGAAGGCATCATTGTCGACTTTGTGGCCGAGTTTTCTGTGCTAAGTGTACTGCAAACTCTATTCCTGCGGCGTCTGATGAGCCAAGGGCTGGAAGAGAAGATTGGGAGCGGATTAGGGTCTGTTATTATTGCTTCAAGCAATGGGAGCAAGGGGTAGCGGCACCCGATAATGGGGCCGCCCCTGCAGCCAGCCCTGGTCTCAGTCCATCACCGTCTGCGACAAGTTTGGCAAGCACCAAGTCCAGCTGCACCTGCCACAGCAGCAGCAGCACTATTGGTTCTACCCCTTATTCGACCGGACCTTACCAGCACGTGCCATATAGTTCTGGTCGTAGCCCTAGTCAATCATCTTCCCAAATTGATTCAGTTCCAGTACAGCAAGACAATGTGACATCGCAGACGAGCATTAGTTCTGATGTAGCCATGGCCGAGCCTTCTCCAAATCAATATGGCTTTTGCTTGAACAGTGATGATGAGGATGATGACTATGGAGTTTATCGTCTAGATTCAGAACCAAGTCATCTCTCTCATGGCAATGACTATTATGGTGCAGTCACTATCGAGGAATTTGCTAGCGTCTATGGACCCCAGAATGTGCATCTTGATGGAGATAACACGAGTTCCTTATTACCTGGGAATTTTGATACAGAGGATGCAGTAGGAATTCAGAAGATTGAGGAAGAACCATACGCACACGATAATGGCGATGAATGTGGAACTTCTCCATATGACCTGCAAAGCACGAATACAGAACCTGTGGATTTCGAGAACAATGGGCTACTTTGGCTCCCTCCGGAGCCTGAGGACGAAGAAGATGAGAGAGAAGCTGTCTTATTTGATGACGACGATTATGATGGTGGTGGTTCTGGTGGTGGTGTAGGGGAGTGGGGTTACCTGGGTTCTTCACATAGCTTTGGTAATGGGGAGTGTCGTACGAGGGAGAAGTCAATTGAGGAGCACAGGAAGGCGATGAAGAATGTGGTGGAAGGTCATTTTAGAGCTTTGGTATCTCAGCTTTTGCAGGTTGAAAATCTACCACTGGGTGATGAAGGCAACAATGAGAGCTGGTTGGACATAATTACTTCGTTGTCGTGGGAAGCTGCCACACTTCTCAAGCCAGATACAAGCAAGGGTGGAGGAATGGACCCTGGGGGATATGTGAAGGTTAAATGCATAGCTTGTGGGCGTCGAACTGATAGTACGGTAGTTAAAGGAGTTGTTTGTAAGAAAAATGTGGCTCATCGGCGAATGACATCAAAAATAGAAAAACCGCGCTTTTTAATCCTTGGAGGAGCTTTGGAATATCAGCGTGTTTCAAACCTCTTGTCAAGTTTCGATACTTTGTTGCAGCAGGAAATGGATCATCTGAAGATGGCAGTAGCAAAGATAGATTCACATCACCCAAGTGTTCTTTTGGTGGAGAAGTCTGTATCTCGGTATGCTCAAGATTACCTTCTTGCGAAAGACATCTCACTTGTTTTGAATATTAAGAGGCCACTTTTAGAGCGAATAGCCCGCTGCACTGGGGCACAAATAGTCCCTTCAATAGATCATCTCACATCACCAAAACTAGGGTTTTGTGACATGTTCCATGTGGAGAAGTTTCTTGAAGTGCATGGCAGTGCTGGGCAAGGTGGGAAGAAATTGACAAAGACTCTGATGTTCTGTGAAGGTTGCCCGAAGCCCTTGGGTGTTACTATATTGCTCCATGGTGCCAATGGGGATGAGTTAAAGAAGGTGAAACATGTTGTCCAATATGGAGTTTTTGCCGCTTATCACTTGGCTCTGGAGACTTCATTTCTAGCTGATGAAGGTGCTTCACTTCCGGAACTTCCTTTGAAGTCCCAGATTACTGTTGCATTACCCGATAAACCATCAAGTATTGACAGGTCTATTTCCACCATACCTGGTTTTAGTGTCCCACCGGCTGGCAAGCCTCAGGGTCCCGATGCTAGCAGGGAACTACAGAAATCTAATCAAAGGTTAATTTCATATAGCAATTCATCTACCACTAGTGGCCCCATTTTGAACATGCAAGGTGCCGATTCAATTTGCTCATCTAAAGCTTGTTCTCAAGCTTTTCTCATAGAACATGCTTTGAGCTCTAGAGAATCTAGATCTCCCTTTACTTCCTTGTCTCCCCAAGAAGAGGATATCACAGAATCTTACCGTAAAGAGCTCCCCTCAATATGTGCCTCTGAAAATAAAATTGATGTGGGTTCTAAAGATTCTTGCCTGGACAATACAGCTCAGGTTGGGGAAGCTTTGGTCAACGGTAGTTTGATCTCTAATTTTCTTGCCACCTCAGAGTCATTAGGACATGGTGGCGGTGCTTTAGCTGCAAATCATGGTGAGACTCCAGAGTTGACATCCATAAAACATCATAGTGATTATCAGAATGAGGAGGTAGGATCTTCAAAGGAAGAGTTTCCACCCTCACCTTCTGACCACCAAAGCATTTTGGTTTCCCTGTCAACACGTTGTGTGTGGAAGGGAACTGTGTGTGAACGGTCCCATCTGTTTCGAATCAAATACTACGGAAGTTTTGATAAGCCTTTGGGGAGGTTCTTACGAGATCATCTGTTTGATCAGAATTACCTTTGTCGTTCATGTGGGATGCCATCAGAAGCGCATGTTCATTGTTATACTCATAGACAAGGGAGCCTTACAATATCTGTTAAGAAGGTCCCAGAGATTTTGCTACCTGGGGAAAGGGAAGGCAAAATTTGGATGTGGCACAGGTGCTTGAAATGTCCTCGAGCCAATGGCTTCCCTCCTGCAACTCGAAGAGTGGTCATGTCAGATGCTGCTTGGGGTTTATCGTTTGGGAAGTTTTTAGAGTTAAGCTTTTCAAATCATGCAGCTGCAAACCGAGTTGCAACTTGTGGTCATTCCCTTCACAGAGATTGTCTTCGGTTTTATGGTTTTGGAAGAATGGTTGCTTGCTTCCGCTATGCCTCCATTCATGTTCATTCTGTTTATCTTCCTCCACAAAAACTCGAATTTAACTATGATAATCAGGAATGGATACAAAAAGAAGCGGAGGAGGTGGGTCATCGGGCAGAGCTTCTCTTTACTGAATTGTGCAATGCTCTTAATCAGATTTTGGAGAAGAGACCAATTTCAGGGACACCAGATGGTGGGAAGAAAGCACCTGAATCAAGTCATCAAATTGTAGAACTTGAAGAGATGCTTCAAAAGGAAAGAGAAGACTTTGAGGAATCATTACGAAAAGCGATGCATAGGGAGGTGAAGTGTGGCCGGCCTGCAATTGATATTCTCGAGATCAATAGATTAAGGAGGCAGTTGCTCTTTCATTCTTACATTTGGGATCAACGCCTAATTCAGGCTGCCAGTTTAAGTAAAAATAGTTTCCAGGAAGGTTTGAGAAGTTCCCTTCCAAAACTCAAGGAGAAACCTATCAGTTCTATGGAGAAGCTTGTCGAGACAAATATAAACTCCAAGCCAGGTAAAGGATTTAGTAGGTGTGATTCCTCTCTTCGGGAAACAAAGCCAGATGTAAGTATTTATCAAGGAGGAGATGTTGGCGGCTTTAGCCAGCCTGAAGGGGAGCAGAAGAAAAATGAAATCGTTCAGAACCTGAATCATAGTAACGAGGCAAAAATTTCCACACGGTCCTCTGAAAATGCCATTGATAAATCTGATCCATTGGAGTCTGGAATAAGTGCACGAAGGGCCCTCTCAGAAGGGAACGAGAGTCTAGTAGTGGCAAATTTATCTGATACCCTTGATGCGGCTTGGACGGGTGAAAGTCACCCAACTAGTACGATTCCTAAAGAGAATGGCTACTCTAAACCTGATTCAACCTTGGTAAATTCTCCGACTATGATGAGAAAAGTTGCATCGAATTCAGATCTGCAAAATTGTGCCGTTGACCAAGCTGGGGTACAGACAACTGCTGCAACTCATTCTCTTAGCTCTACATCATCTCTCAAGGTATTTGACAAAAGTTATTCCCTGAATGCACAAAAGATCATTATTGGTGAATACAATCCCGTCAATGTTCCAATGTTCCGGGAATCGGAAAGGCAAAGCGGTGCCAGGTTGTTACTGCCAATTGGTGTTAATGACACTGTCATCCCTGTTTTTGACGATGAACCCACTAGTGTTATTGCCTATGCACTAGTCTCGCCAGATTATCATGTGCAGATTTCCGAGTCTGAGAGACCAAGAGATGCTATGGATGGTTCAGTCTCAGCACCTCTTTTTGATTCCGCAAATCTTCTCTCACTTAGCTCTTTTGATGAGTCGCTTTCTGAGACCTACAGAAATCTTGGTTCTTCTGATGAGAGCATGTCATCTGTGTCGCGGTCGTGGAGCTCTCAAGCTTTGGACTCACTTTTGTCAAAGGACATTCATGCTAGAGTTTCTTTTACAGATGATGGCCCCCTTGGTAAGGTGAAGTATACCGTGACTTGTTACTATGCAACGCGGTTTGAGGCTTTAAGGAGGACGTGTTGCCCTTCTGAAAGAGATTTCGTGCGATCTCTTAGCCGCTGTAAGAAGTGGGGCGCCCAGGGTGGCAAGAGCAACGTTTTCTTTGCAAAAACCTTGGATGATCGATTTATCATCAAACAAGTTACAAAGATAGAGCTGGAATCATTTATCAAGTTTGCACCATCTTATTTCAAGTATTTGTCTGAGTCAATCAGTACTCGAAGCCCGACTTGCCTGGCAAAGATCCTGGGTATTTATCAGGTTTCGTCAAAGCATGGTAAAGCAGGGAAAGAGTCCAAGATGGATGTTTTAGTAATGGAGAATTTATTATTTAGACGGAATGTCACGCGGCTTTATGATCTGAAGGGATCTTCTCGATCACGTTATAATCCAGATACAAGTGGGAGCAATAAAGTTCTACTCGACCAGAATCTGATTGAAGCAATGCCAACTTCTCCAATTTTTGTGGGGAGCAAGGCAAAGCGGCTACTAGAGAGAGCTGTCTGGAATGATACTGCATTCCTTGCTTCGATTGATGTCATGGACTACTCGTTACTGGTCGGTGTGGACGAGGAAAAGGATGAGCTGGTTCTGGGGATCATTGATTTTATGAGACAGTACACATGGGACAAGCACCTTGAAACTTGGGTGAAGACCTCCGGGATTCTCGGCGGACCCAAGAACACGTCTCCAACCGTGATTTCACCCCAGCAATACAAGAAACGATTCAGGAAAGCTATGACATCTTACTTCCTCATGGTGCCCGATCAGTGGTCTCCCCTAACGATCGTTCCGAGCAGGTCGCAATCTGACCTTGGTGAAGAAACTGCGCAAGATCCGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1821

Amino Acids

201.25

Weight (kDa)

5.84

Isoelectric Point (pI)

50.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	4.9e-17	FYVE zinc finger
Cpn60_TCP1	PF00118	407 - 655	5.8e-29	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1566 - 1732	4.1e-34	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1732 - 1788	3.9e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240
fragaria_vesca	FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530
malus_domestica	MD02G1103900.v1.1 MD15G1224300.v1.1
prunus_persica	Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1
pyrus_communis	pycom02g08170 pycom15g19930 pycom15g19940
rosa_chinensis	RchiOBHm_Chr2g0096101
rosa_laevigata	RLG00000016625
rosa_multiflora	Rmu_sc0005996.1_g000006
rosa_roxburghii	Rroxscaffold_2G00145750
rosa_rugosa	Rorug02G0057000 Rorug02G0057100 Rorug02G0057200 Rorug02G0057200
rosa_samantha	Rh2AG104000 Rh2BG106100 Rh2CG108000 Rh2DG106700
rosa_wichuraiana	Rw2G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	5004
AarI	CACCTGC	1 cut(s)	419
Acc16I	TGCGCA	1 cut(s)	5448
Acc36I	ACCTGC	4 cut(s)	419, 900, 1171, 5406
AccB1I	GGYRCC	6 cut(s)	319, 1871, 2198, 4217, 4691, 5377
AccB7I	CCANNNNNTGG	3 cut(s)	248, 2087, 3299
AccBSI	CCGCTC	1 cut(s)	268
AccI	GTMKAC	2 cut(s)	175, 4586
AccII	CGCG	4 cut(s)	1432, 4489, 4612, 4966
AccIII	TCCGGA	2 cut(s)	952, 1984
AclI	AACGTT	1 cut(s)	4713
AcoI	YGGCCR	3 cut(s)	184, 585, 3418
AcuI	CTGAAG	3 cut(s)	1547, 3744, 5000
AcvI	CACGTG	1 cut(s)	472
AcyI	GRCGYC	3 cut(s)	230, 1345, 4692
AdeI	CACNNNGTG	2 cut(s)	1202, 2642
AfaI	GTAC	8 cut(s)	208, 534, 1093, 1360, 3946, 4068, 4834, 5237
AflIII	ACRYGT	5 cut(s)	1743, 1903, 2636, 4634, 5301
AhdI	GACNNNNNGTC	1 cut(s)	4958
AhlI	ACTAGT	4 cut(s)	2171, 3941, 4283, 4303
AjiI	CACGTC	2 cut(s)	4635, 5304
AjuI	GAANNNNNNNTTGG	4 cut(s)	244, 276, 5302, 5334
AleI	CACNNNNGTG	1 cut(s)	1563
Alw21I	GWGCWC	5 cut(s)	1119, 2264, 2340, 3850, 4502
Alw44I	GTGCAC	1 cut(s)	3846
AlwNI	CAGNNNCTG	4 cut(s)	914, 2966, 3760, 5050
Ama87I	CYCGRG	2 cut(s)	2916, 3440
Aor13HI	TCCGGA	2 cut(s)	952, 1984
AoxI	GGCC	9 cut(s)	184, 333, 585, 1652, 2176, 3418, 3422, 3855, 4564
ApaI	GGGCCC	1 cut(s)	3859
ApaLI	GTGCAC	1 cut(s)	3846
ArsI	GACNNNNNNTTYG	2 cut(s)	1469, 1501
Asp700I	GAANNNNTTC	3 cut(s)	2586, 3750, 5342
AspLEI	GCGC	4 cut(s)	1434, 2791, 4694, 5449
AsuC2I	CCSGG	2 cut(s)	4196, 5278
AsuII	TTCGAA	1 cut(s)	2683
AsuNHI	GCTAGC	2 cut(s)	731, 2111
AvaI	CYCGRG	2 cut(s)	2916, 3440
AvaII	GGWCC	9 cut(s)	66, 458, 743, 1303, 2102, 2670, 2845, 3795, 5291
AxyI	CCTNAGG	2 cut(s)	960, 2097
BaeGI	GKGCMC	5 cut(s)	8, 1690, 3850, 3859, 5382
BamHI	GGATCC	1 cut(s)	54
BanI	GGYRCC	6 cut(s)	319, 1871, 2198, 4217, 4691, 5377
BanII	GRGCYC	4 cut(s)	2264, 2340, 3859, 4502
BauI	CACGAG	1 cut(s)	775
BbrPI	CACGTG	1 cut(s)	472
BbsI	GAAGAC	3 cut(s)	3062, 3375, 5276
Bbv12I	GWGCWC	5 cut(s)	1119, 2264, 2340, 3850, 4502
BceAI	ACGGC	1 cut(s)	4034
BcgI	CGANNNNNNTGC	6 cut(s)	155, 189, 710, 744, 4290, 4324
BciVI	GTATCC	1 cut(s)	3180
BclI	TGATCA	2 cut(s)	106, 2746
BcnI	CCSGG	2 cut(s)	4196, 5278
BcuI	ACTAGT	4 cut(s)	2171, 3941, 4283, 4303
BfmI	CTRYAG	4 cut(s)	115, 342, 2123, 4444
BfoI	RGCGCY	1 cut(s)	4695
BfuAI	ACCTGC	4 cut(s)	419, 900, 1171, 5406
BfuI	GTATCC	1 cut(s)	3180
BglII	AGATCT	2 cut(s)	2276, 4033
BmcAI	AGTACT	1 cut(s)	4834
Bme18I	GGWCC	9 cut(s)	66, 458, 743, 1303, 2102, 2670, 2845, 3795, 5291
BmeRI	GACNNNNNGTC	1 cut(s)	4958
BmeT110I	CYCGRG	2 cut(s)	2916, 3440
BmgBI	CACGTC	2 cut(s)	4635, 5304
BmrI	ACTGGG	2 cut(s)	1208, 1692
BmtI	GCTAGC	2 cut(s)	735, 2115
BmuI	ACTGGG	2 cut(s)	1208, 1692
BpiI	GAAGAC	3 cut(s)	3062, 3375, 5276
BplI	GAGNNNNNCTC	2 cut(s)	2327, 2359
BpmI	CTGGAG	2 cut(s)	1967, 2502
Bpu10I	CCTNAGC	1 cut(s)	2409
Bpu14I	TTCGAA	1 cut(s)	2683
BpuEI	CTTGAG	6 cut(s)	1256, 1587, 2217, 3551, 4097, 4488
BpuMI	CCSGG	2 cut(s)	4196, 5278
Bsa29I	ATCGAT	1 cut(s)	4741
BsaAI	YACGTR	1 cut(s)	472
BsaBI	GATNNNNATC	1 cut(s)	1554
BsaHI	GRCGYC	3 cut(s)	230, 1345, 4692
BsaI	GGTCTC	5 cut(s)	362, 3268, 4339, 4433, 5397
BsaWI	WCCGGW	3 cut(s)	455, 952, 1984
BsaXI	ACNNNNNCTCC	8 cut(s)	930, 960, 3398, 3428, 3687, 3717, 4621, 4651
Bse118I	RCCGGY	2 cut(s)	2083, 3420
Bse1I	ACTGG	5 cut(s)	530, 1203, 1687, 3513, 5178
Bse21I	CCTNAGG	2 cut(s)	960, 2097
Bse3DI	GCAATG	4 cut(s)	305, 700, 3253, 5067
Bse8I	GATNNNNATC	1 cut(s)	1554
BseAI	TCCGGA	2 cut(s)	952, 1984
BseCI	ATCGAT	1 cut(s)	4741
BseJI	GATNNNNATC	1 cut(s)	1554
BseMI	GCAATG	4 cut(s)	305, 700, 3253, 5067
BseNI	ACTGG	5 cut(s)	530, 1203, 1687, 3513, 5178
BseRI	GAGGAG	5 cut(s)	1127, 1464, 2576, 3218, 3711
BseSI	GKGCMC	5 cut(s)	8, 1690, 3850, 3859, 5382
BseYI	CCCAGC	3 cut(s)	1784, 4061, 5325
BsgI	GTGCAG	4 cut(s)	391, 729, 1663, 4346
Bsh1236I	CGCG	4 cut(s)	1432, 4489, 4612, 4966
Bsh1285I	CGRYCG	3 cut(s)	456, 4493, 5406
BshFI	GGCC	9 cut(s)	186, 335, 587, 1654, 2178, 3420, 3424, 3857, 4566
BshNI	GGYRCC	6 cut(s)	319, 1871, 2198, 4217, 4691, 5377
BshVI	ATCGAT	1 cut(s)	4741
BsiEI	CGRYCG	3 cut(s)	456, 4493, 5406
BsiHKAI	GWGCWC	5 cut(s)	1119, 2264, 2340, 3850, 4502
BsiHKCI	CYCGRG	2 cut(s)	2916, 3440
BsiSI	CCGG	7 cut(s)	456, 953, 1985, 2084, 3421, 4195, 5277
BsiWI	CGTACG	1 cut(s)	1091
BslFI	GGGAC	9 cut(s)	104, 1682, 1987, 2063, 2088, 2656, 2831, 3301, 5258
BsmBI	CGTCTC	1 cut(s)	5310
BsmFI	GGGAC	9 cut(s)	104, 1682, 1987, 2063, 2088, 2656, 2831, 3301, 5258
BsmI	GAATGC	2 cut(s)	4147, 5135
BsnI	GGCC	9 cut(s)	186, 335, 587, 1654, 2178, 3420, 3424, 3857, 4566
Bso31I	GGTCTC	5 cut(s)	362, 3268, 4339, 4433, 5397
BsoBI	CYCGRG	2 cut(s)	2916, 3440
Bsp119I	TTCGAA	1 cut(s)	2683
Bsp120I	GGGCCC	1 cut(s)	3855
Bsp1286I	GDGCHC	9 cut(s)	8, 1119, 1690, 2264, 2340, 3850, 3859, 4502, 5382
Bsp13I	TCCGGA	2 cut(s)	952, 1984
Bsp19I	CCATGG	2 cut(s)	582, 1867
BspANI	GGCC	9 cut(s)	186, 335, 587, 1654, 2178, 3420, 3424, 3857, 4566
BspDI	ATCGAT	1 cut(s)	4741
BspEI	TCCGGA	2 cut(s)	952, 1984
BspFNI	CGCG	4 cut(s)	1432, 4489, 4612, 4966
BspMAI	CTGCAG	1 cut(s)	346
BspMI	ACCTGC	4 cut(s)	419, 900, 1171, 5406
BspOI	GCTAGC	2 cut(s)	735, 2115
BspT104I	TTCGAA	1 cut(s)	2683
BspT107I	GGYRCC	6 cut(s)	319, 1871, 2198, 4217, 4691, 5377
BspTNI	GGTCTC	5 cut(s)	362, 3268, 4339, 4433, 5397
BsrBI	CCGCTC	1 cut(s)	268
BsrDI	GCAATG	4 cut(s)	305, 700, 3253, 5067
BsrFI	RCCGGY	2 cut(s)	2083, 3420
BsrI	ACTGG	5 cut(s)	530, 1203, 1687, 3513, 5178
BssAI	RCCGGY	2 cut(s)	2083, 3420
BssNAI	GTATAC	1 cut(s)	4587
BssNI	GRCGYC	3 cut(s)	230, 1345, 4692
BssSI	CACGAG	1 cut(s)	775
BssT1I	CCWWGG	9 cut(s)	242, 582, 1444, 1845, 1867, 3987, 4570, 4731, 5431
Bst1107I	GTATAC	1 cut(s)	4587
Bst2BI	CACGAG	1 cut(s)	775
Bst6I	CTCTTC	7 cut(s)	247, 2301, 2577, 2941, 3266, 3339, 3663
BstACI	GRCGYC	3 cut(s)	230, 1345, 4692
BstAPI	GCANNNNNTGC	1 cut(s)	5141
BstBAI	YACGTR	1 cut(s)	472
BstBI	TTCGAA	1 cut(s)	2683
BstDSI	CCRYGG	2 cut(s)	582, 1867
BstEII	GGTNACC	1 cut(s)	1049
BstENI	CCTNNNNNAGG	2 cut(s)	3503, 4574
BstFNI	CGCG	4 cut(s)	1432, 4489, 4612, 4966
BstH2I	RGCGCY	1 cut(s)	4695
BstHHI	GCGC	4 cut(s)	1434, 2791, 4694, 5449
BstMCI	CGRYCG	3 cut(s)	456, 4493, 5406
BstNSI	RCATGY	6 cut(s)	1747, 1907, 2194, 2255, 2795, 4477
BstPI	GGTNACC	1 cut(s)	1049
BstSFI	CTRYAG	4 cut(s)	115, 342, 2123, 4444
BstSLI	GKGCMC	5 cut(s)	8, 1690, 3850, 3859, 5382
BstUI	CGCG	4 cut(s)	1432, 4489, 4612, 4966
BstV2I	GAAGAC	3 cut(s)	3062, 3375, 5276
BstX2I	RGATCY	7 cut(s)	54, 2276, 2570, 4033, 4860, 4985, 5452
BstYI	RGATCY	7 cut(s)	54, 2276, 2570, 4033, 4860, 4985, 5452
BstZ17I	GTATAC	1 cut(s)	4587
Bsu15I	ATCGAT	1 cut(s)	4741
Bsu36I	CCTNAGG	2 cut(s)	960, 2097
BsuI	GTATCC	1 cut(s)	3180
BsuRI	GGCC	9 cut(s)	186, 335, 587, 1654, 2178, 3420, 3424, 3857, 4566
BsuTUI	ATCGAT	1 cut(s)	4741
BtgI	CCRYGG	2 cut(s)	582, 1867
BtgZI	GCGATG	4 cut(s)	537, 879, 1142, 3410
BtrI	CACGTC	2 cut(s)	4635, 5304
BtsI	GCAGTG	1 cut(s)	1786
BtsIMutI	CAGTG	6 cut(s)	631, 1196, 1680, 1786, 4251, 5393
BveI	ACCTGC	4 cut(s)	419, 900, 1171, 5406
CaiI	CAGNNNCTG	4 cut(s)	914, 2966, 3760, 5050
CfoI	GCGC	4 cut(s)	1434, 2791, 4694, 5449
Cfr10I	RCCGGY	2 cut(s)	2083, 3420
ClaI	ATCGAT	1 cut(s)	4741
CseI	GACGC	3 cut(s)	219, 724, 1334
CsiI	ACCWGGT	1 cut(s)	2065
Csp6I	GTAC	8 cut(s)	207, 533, 1092, 1359, 3945, 4067, 4833, 5236
CspCI	CAANNNNNGTGG	2 cut(s)	5064, 5099
CviQI	GTAC	8 cut(s)	207, 533, 1092, 1359, 3945, 4067, 4833, 5236
DinI	GGCGCC	1 cut(s)	4693
DraIII	CACNNNGTG	2 cut(s)	1202, 2642
DriI	GACNNNNNGTC	1 cut(s)	4958
EaeI	YGGCCR	3 cut(s)	184, 585, 3418
Eam1104I	CTCTTC	7 cut(s)	247, 2301, 2577, 2941, 3266, 3339, 3663
Eam1105I	GACNNNNNGTC	1 cut(s)	4958
EarI	CTCTTC	7 cut(s)	247, 2301, 2577, 2941, 3266, 3339, 3663
EciI	GGCGGA	1 cut(s)	5304
Ecl136II	GAGCTC	3 cut(s)	2262, 2338, 4500
Eco130I	CCWWGG	9 cut(s)	242, 582, 1444, 1845, 1867, 3987, 4570, 4731, 5431
Eco24I	GRGCYC	4 cut(s)	2264, 2340, 3859, 4502
Eco31I	GGTCTC	5 cut(s)	362, 3268, 4339, 4433, 5397
Eco32I	GATATC	1 cut(s)	2314
Eco47I	GGWCC	9 cut(s)	66, 458, 743, 1303, 2102, 2670, 2845, 3795, 5291
Eco53kI	GAGCTC	3 cut(s)	2262, 2338, 4500
Eco57I	CTGAAG	3 cut(s)	1547, 3744, 5000
Eco72I	CACGTG	1 cut(s)	472
Eco81I	CCTNAGG	2 cut(s)	960, 2097
Eco88I	CYCGRG	2 cut(s)	2916, 3440
Eco91I	GGTNACC	1 cut(s)	1049
EcoICRI	GAGCTC	3 cut(s)	2262, 2338, 4500
EcoNI	CCTNNNNNAGG	2 cut(s)	3503, 4574
EcoO109I	RGGNCCY	4 cut(s)	2102, 2845, 3855, 3856
EcoO65I	GGTNACC	1 cut(s)	1049
EcoRI	GAATTC	2 cut(s)	822, 4027
EcoRV	GATATC	1 cut(s)	2314
EcoT14I	CCWWGG	9 cut(s)	242, 582, 1444, 1845, 1867, 3987, 4570, 4731, 5431
EcoT22I	ATGCAT	2 cut(s)	1334, 3402
EcoT38I	GRGCYC	4 cut(s)	2264, 2340, 3859, 4502
EgeI	GGCGCC	1 cut(s)	4693
EheI	GGCGCC	1 cut(s)	4693
ErhI	CCWWGG	9 cut(s)	242, 582, 1444, 1845, 1867, 3987, 4570, 4731, 5431
Esp3I	CGTCTC	1 cut(s)	5310
FalI	AAGNNNNNCTT	8 cut(s)	1596, 1628, 2808, 2840, 3336, 3368, 4831, 4863
FaqI	GGGAC	9 cut(s)	104, 1682, 1987, 2063, 2088, 2656, 2831, 3301, 5258
FauI	CCCGC	1 cut(s)	1682
FauNDI	CATATG	1 cut(s)	886
FbaI	TGATCA	2 cut(s)	106, 2746
FblI	GTMKAC	2 cut(s)	175, 4586
FriOI	GRGCYC	4 cut(s)	2264, 2340, 3859, 4502
FseI	GGCCGGCC	1 cut(s)	3424
FspI	TGCGCA	1 cut(s)	5448
GlaI	GCGC	4 cut(s)	1433, 2790, 4693, 5448
GsaI	CCCAGC	3 cut(s)	1788, 4065, 5329
GsuI	CTGGAG	2 cut(s)	1967, 2502
HaeII	RGCGCY	1 cut(s)	4695
HaeIII	GGCC	9 cut(s)	186, 335, 587, 1654, 2178, 3420, 3424, 3857, 4566
HapII	CCGG	7 cut(s)	456, 953, 1985, 2084, 3421, 4195, 5277
HgaI	GACGC	3 cut(s)	219, 724, 1334
HhaI	GCGC	4 cut(s)	1434, 2791, 4694, 5449
Hin1I	GRCGYC	3 cut(s)	230, 1345, 4692
Hin6I	GCGC	4 cut(s)	1432, 2789, 4692, 5447
HinP1I	GCGC	4 cut(s)	1432, 2789, 4692, 5447
HincII	GTYRAC	6 cut(s)	37, 176, 2431, 2526, 2634, 4054
HindII	GTYRAC	6 cut(s)	37, 176, 2431, 2526, 2634, 4054
HindIII	AAGCTT	6 cut(s)	2223, 2235, 2421, 3004, 3596, 4506
HpaII	CCGG	7 cut(s)	456, 953, 1985, 2084, 3421, 4195, 5277
Hpy99I	CGWCG	2 cut(s)	1007, 1350
HpyCH4IV	ACGT	6 cut(s)	471, 2638, 4634, 4713, 4999, 5303
HpySE526I	ACGT	6 cut(s)	471, 2638, 4634, 4713, 4999, 5303
Hsp92I	GRCGYC	3 cut(s)	230, 1345, 4692
HspAI	GCGC	4 cut(s)	1432, 2789, 4692, 5447
KasI	GGCGCC	1 cut(s)	4691
KflI	GGGWCCC	1 cut(s)	2102
Kpn2I	TCCGGA	2 cut(s)	952, 1984
KroI	GCCGGC	1 cut(s)	3420
KroNI	GCCGGC	1 cut(s)	3422
Ksp22I	TGATCA	2 cut(s)	106, 2746
MabI	ACCWGGT	1 cut(s)	2065
MaeII	ACGT	6 cut(s)	471, 2638, 4634, 4713, 4999, 5303
MbiI	CCGCTC	1 cut(s)	268
MfeI	CAATTG	3 cut(s)	1107, 3429, 4236
MflI	RGATCY	7 cut(s)	54, 2276, 2570, 4033, 4860, 4985, 5452
MhlI	GDGCHC	9 cut(s)	8, 1119, 1690, 2264, 2340, 3850, 3859, 4502, 5382
Mly113I	GGCGCC	1 cut(s)	4692
MmeI	TCCRAC	3 cut(s)	1209, 4025, 5333
Mph1103I	ATGCAT	2 cut(s)	1334, 3402
MroI	TCCGGA	2 cut(s)	952, 1984
MroNI	GCCGGC	1 cut(s)	3420
MroXI	GAANNNNTTC	3 cut(s)	2586, 3750, 5342
MslI	CAYNNNNRTG	6 cut(s)	693, 762, 858, 1563, 1779, 2544
MspA1I	CMGCKG	4 cut(s)	405, 1677, 3023, 4677
MspI	CCGG	7 cut(s)	456, 953, 1985, 2084, 3421, 4195, 5277
MunI	CAATTG	3 cut(s)	1107, 3429, 4236
Mva1269I	GAATGC	2 cut(s)	4147, 5135
MvnI	CGCG	4 cut(s)	1432, 4489, 4612, 4966
NaeI	GCCGGC	1 cut(s)	3422
NarI	GGCGCC	1 cut(s)	4692
NciI	CCSGG	2 cut(s)	4196, 5278
NcoI	CCATGG	2 cut(s)	582, 1867
NdeI	CATATG	1 cut(s)	886
NgoMIV	GCCGGC	1 cut(s)	3420
NheI	GCTAGC	2 cut(s)	731, 2111
NmeAIII	GCCGAG	3 cut(s)	212, 613, 5265
NmuCI	GTSAC	7 cut(s)	134, 547, 712, 1739, 3932, 4591, 4960
NsbI	TGCGCA	1 cut(s)	5448
NsiI	ATGCAT	2 cut(s)	1334, 3402
NspI	RCATGY	6 cut(s)	1747, 1907, 2194, 2255, 2795, 4477
NspV	TTCGAA	1 cut(s)	2683
OliI	CACNNNNGTG	1 cut(s)	1563
PaeR7I	CTCGAG	2 cut(s)	2916, 3440
PaqCI	CACCTGC	1 cut(s)	419
PasI	CCCWGGG	2 cut(s)	1307, 4696
PciI	ACATGT	2 cut(s)	1743, 1903
PcsI	WCGNNNNNNNCGW	3 cut(s)	861, 5184, 5408
PctI	GAATGC	2 cut(s)	4147, 5135
PdiI	GCCGGC	1 cut(s)	3422
PdmI	GAANNNNTTC	3 cut(s)	2586, 3750, 5342
Pfl23II	CGTACG	1 cut(s)	1091
PflFI	GACNNNGTC	1 cut(s)	4253
PflMI	CCANNNNNTGG	3 cut(s)	248, 2087, 3299
PfoI	TCCNGGA	3 cut(s)	3534, 4194, 5276
Ple19I	CGATCG	1 cut(s)	5406
PluTI	GGCGCC	1 cut(s)	4695
PmaCI	CACGTG	1 cut(s)	472
PmlI	CACGTG	1 cut(s)	472
Ppu21I	YACGTR	1 cut(s)	472
PpuMI	RGGWCCY	2 cut(s)	2102, 2845
PscI	ACATGT	2 cut(s)	1743, 1903
PsiI	TTATAA	1 cut(s)	5004
Psp124BI	GAGCTC	3 cut(s)	2264, 2340, 4502
Psp1406I	AACGTT	1 cut(s)	4713
Psp5II	RGGWCCY	2 cut(s)	2102, 2845
PspCI	CACGTG	1 cut(s)	472
PspEI	GGTNACC	1 cut(s)	1049
PspFI	CCCAGC	3 cut(s)	1784, 4061, 5325
PspLI	CGTACG	1 cut(s)	1091
PspOMI	GGGCCC	1 cut(s)	3855
PspPPI	RGGWCCY	2 cut(s)	2102, 2845
PspXI	VCTCGAGB	1 cut(s)	2916
PsrI	GAACNNNNNNTAC	2 cut(s)	1342, 1374
PstI	CTGCAG	1 cut(s)	346
PstNI	CAGNNNCTG	4 cut(s)	914, 2966, 3760, 5050
PsuI	RGATCY	7 cut(s)	54, 2276, 2570, 4033, 4860, 4985, 5452
PsyI	GACNNNGTC	1 cut(s)	4253
PvuI	CGATCG	1 cut(s)	5406
PvuII	CAGCTG	2 cut(s)	405, 3023
RigI	GGCCGGCC	1 cut(s)	3424
RsaI	GTAC	8 cut(s)	208, 534, 1093, 1360, 3946, 4068, 4834, 5237
RsaNI	GTAC	8 cut(s)	207, 533, 1092, 1359, 3945, 4067, 4833, 5236
RseI	CAYNNNNRTG	6 cut(s)	693, 762, 858, 1563, 1779, 2544
SacI	GAGCTC	3 cut(s)	2264, 2340, 4502
SalI	GTCGAC	1 cut(s)	174
ScaI	AGTACT	1 cut(s)	4834
SduI	GDGCHC	9 cut(s)	8, 1119, 1690, 2264, 2340, 3850, 3859, 4502, 5382
SexAI	ACCWGGT	1 cut(s)	2065
SfcI	CTRYAG	4 cut(s)	115, 342, 2123, 4444
SfoI	GGCGCC	1 cut(s)	4693
Sfr274I	CTCGAG	2 cut(s)	2916, 3440
SfuI	TTCGAA	1 cut(s)	2683
SinI	GGWCC	9 cut(s)	66, 458, 743, 1303, 2102, 2670, 2845, 3795, 5291
SlaI	CTCGAG	2 cut(s)	2916, 3440
SmiMI	CAYNNNNRTG	6 cut(s)	693, 762, 858, 1563, 1779, 2544
SmlI	CTYRAG	8 cut(s)	1271, 1602, 2232, 2916, 3440, 3566, 4112, 4503
SmoI	CTYRAG	8 cut(s)	1271, 1602, 2232, 2916, 3440, 3566, 4112, 4503
SpeI	ACTAGT	4 cut(s)	2171, 3941, 4283, 4303
SspDI	GGCGCC	1 cut(s)	4691
SspI	AATATT	2 cut(s)	153, 1645
SstI	GAGCTC	3 cut(s)	2264, 2340, 4502
StyI	CCWWGG	9 cut(s)	242, 582, 1444, 1845, 1867, 3987, 4570, 4731, 5431
TaiI	ACGT	6 cut(s)	474, 2641, 4637, 4716, 5002, 5306
TatI	WGTACW	4 cut(s)	206, 532, 4832, 5235
TscAI	CASTG	6 cut(s)	631, 1203, 1687, 1786, 4258, 5393
TseFI	GTSAC	7 cut(s)	134, 547, 712, 1739, 3932, 4591, 4960
Tsp45I	GTSAC	7 cut(s)	134, 547, 712, 1739, 3932, 4591, 4960
TspGWI	ACGGA	2 cut(s)	2712, 4969
TspRI	CASTG	6 cut(s)	631, 1203, 1687, 1786, 4258, 5393
Tth111I	GACNNNGTC	1 cut(s)	4253
Van91I	CCANNNNNTGG	3 cut(s)	248, 2087, 3299
VneI	GTGCAC	1 cut(s)	3846
VpaK11BI	GGWCC	9 cut(s)	66, 458, 743, 1303, 2102, 2670, 2845, 3795, 5291
XagI	CCTNNNNNAGG	2 cut(s)	3503, 4574
XbaI	TCTAGA	3 cut(s)	661, 2264, 2273
XceI	RCATGY	6 cut(s)	1747, 1907, 2194, 2255, 2795, 4477
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1874
XhoI	CTCGAG	2 cut(s)	2916, 3440
XmiI	GTMKAC	2 cut(s)	175, 4586
XmnI	GAANNNNTTC	3 cut(s)	2586, 3750, 5342
ZrmI	AGTACT	1 cut(s)	4834
Zsp2I	ATGCAT	2 cut(s)	1334, 3402

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.