Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0002065

Physical Location & Seq

Genomic Coordinates Forward (+)

8972348 .. 8980636

Gene Structure 8289 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG104000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5463 bp

ATGGGCACCCCTGACAACAAATTGTCAGAGATAGTTGACATTTTCAAGTCTTGGATGCCCAGGAGAACCGAGCCAACCAATGTGTCCAGGGACTTTTGGATGCCCGATCACAGCTGTAGGGTATGCTACGACTGTGATTCCCAGTTCACAGTATTTAATCGAAGGCATCATTGCCGCCTTTGTGGCCGAGTTTTCTGCGCTAGGTGTACTGCAAACACTATTCCTGCCCCATCTGATGAGCCAAGAATTGGTAGGGAAGACGGGGAGAGGATTAGGGTCTGTAATTTTTGCTTCAAGCAATGGGAGCAAGGGATAGCAGCAGTTGATAATGGGCCCCCTGAACCTAGCCCTGGCCTCAGTCCATCACCATCTACAACAAGCTTGGTCAGTACCAACACAAGCTGCACCTACCACAGTAGCAGCAGCACTGTTGGTTCTACCCCTTATTCGACTGGACCTTACCAGCACGTGCCCTATAGTTCTGGTCTTAGCCCCAGACAATCTTGCCAAGATGATTCAGTTACTGGCCTGCAAGACAATGTAACATCTCAGAGGAGCATTAGTTCTGACACTGCCATGGCAGAGTCGTCTCCCAACCAGTTTGGCTTTTGCATGGACAGGAGTGATGATGAGGATGATGACTATGGTTATCATTCAGATTCAGAATCAAGACATTTCACCCATGCCAATGACTATTATGGTGCAATCAATAACGAGGAGTTTGATAGTGTGTATGAACCACAGAAGGTGCATTCTGATGGAGAAACCACAGATGCAAAATGTTTGAGCTCCTTTTCACCTGAGAACTTTGATACACAGAGTGTAGTGGGAACGAAACTTGGGGAAGAATCAGATTATCATATTAATTGTGATGAATGTGAAACTTCTCCATATGAGATGGAGACTACAAATGCAGAACCGGTGGATTTTGAGAATAATGGGCTACTTTGGCTCCCACCAGAACCTGAGGATGAAGAAGATGACAGAGAAGCTGCCTTATTTGAGGATGATGACGACGATGAGGGTGGTGATGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGCTACAGGGGAGTGGGGATATCTTCATTCTTCAAACAGTTTTGGTGGTGGGGAGTGTCGTACTAGGGAGAAGTCAATTGAGGAGCACAGGAAGGCCATGAAGAATGTGGTGGAAGGTCATTTTAGAGCTCTGGTATCTCAGCTCTTGCAGGTTGAAAACCTGCCTTCGATTGATGAAAATTACAAAGAGACTTGGTTGGACATAATTACGTCTTTGTCATGGGAAGCTGCCACACTTCTTAAGCCAGATACGAGCAAAGGGGGAGGAATGGATCCTGGGGGATATGTGAAGGTTAAATGCATAGCTTGTGGGAGTCGGAGCGAGAGCATGGTGGTTAAAGGAGTTGTTTGCAAGAAAAACGTGGCTCATCGACGAATGACTTCAAAGATAGAAAAACCACGTTTCTTGATCCTTGGAGGAGCTTTGGAATATCAGCGTGTATCAAACCACTTGTCCAGTTTCGATACTTTGTTGCAGCAGGAGATGGACCATCTGAAGATGGCAGTTGCTAAGATCGATGCACATCACCCTAATGTTTTATTGGTGGAGAAGTCTGTATCTCGGTATGCTCAAGATTACCTTCTTGCGAAAGACATATCACTCGTTCTGAATATTAAGAGGCCACTTTTGGAGCGAATATCTCGCTGCACTGGGGCACAAATTGTCCCTTCGATAGATCATCTTACATCACCAAAACTAGGGTATTGCGACATGTTCCATGTGGAGAAATTTTTTGAAGTGCATGGCAGTGCTGGACAAGGTGGGAAGAAATTAACAAAGACTCTGATGTTCTTTGAAGGTTGTCCAAAGCCATTGGGTGTTACTATATTGCTGAAGGGTGCCAATGGGGATGAGTTAAAAAAGGTGAAACATGTTGTGCAGTATGGAGTTTTTGCAGCTTATCACTTGGCTTTGGAGACCTCATTTTTAGCTGATGAAGGAGCTTCCCTTCCAGAACTTCCTCTGAAGTCTGTGGTAACTGTATTGCCTGATAAACCGTCGAGTATTGACAGGTCCATTTCGACCATACCTGGTTTTAGTGTACCAGCAGCTGGCAAGCCTCAGAGTTCCAATGCTAGCAGTGAACTGCAGAACTCAAATAAAGGCCTCATTTCAGATAGTGGCTCATTTGCCCAAGTTGCCTCCATTTTAAAGAATGAAGGCACCAATTTGGTCTGCTTACCTAAAGCTCCATGTTCTCAACCTTCCCCCATAAAACATACCCCGAGTCCTATAGAATATACATCTTCTTTCACTTCCTTGTCTCCCCCAGGACAGGATACGACAGACTCCTGCCATAAAGAGCTTTCTTCAATTTGTGGCTCTGAGGACAACAAGGATGTAAGTTCTAAAGAATCTTGTCCAGCCAAAACAGCCAAGGCAGGCGAAGCTTTAGTTCACGATAGTTTGATCTCTAATTCTTTTAGCACCTCAGAGGCATTTGGACATGGTGGTGGAATTGGCAATGCTGATGGCGTTGCTTTAGCTGCAAATCTCAGAGAGACTCCAGAGCTTCCATCCATAAAATATCATACTGATAATCAGAATGAGGAGGTGGGATCTTCAAAAGAAGAGTTTCCCCCCTCACCTTCAGACCACCAGAGCATTTTGGTTTCCCTATCAACACGTTGTGTGTGGAAAGGAACTGTATGTGAACGAGCCCATCTTTTTCGAATCAAATACTATGGAAGCTTTGATAAGCCTCTGGGGAGGTTTTTACGAGACCATCTTTTTGATCAGGGTTACCTCTGTCGTTCATGTGGGATGCCCTCAGAAGCGCATATTCATTGTTATACTCATAGACAAGGAAGCCTTACAATATCTGTTAAGAAACTCCCTGAGATTTTCCTGCCAGGGGAAAAGGAAGGAAAAATATGGATGTGGCATAGGTGCTTGAGATGTCCTCGGACCAGTGGCTTCCCTCCAGCCACTCGTAGAGTTGTAATGTCAGATGCTGCTTGGGGTTTATCTTTTGGGAAATTTCTGGAATTGAGCTTTTCAAATCATGCAGCTGCAAACAGAGTTGCAAGCTGTGGTCATTCCCTGCACAGAGATTGTCTTCGGTTTTATGGGTTTGGGAGAATGGTTGCTTGCTTCCGCTATGCCTCAATTCATATTCACTCTGTTTGTCTGCCCCCACCAAAACTTGAATTTTACTATGATAATCAGGAATGGATACAAAAAGAAGCATACGAGGTGGGCAACCGGGCGGAGCTTCTATTTACTGAATTACATAGTGCTCTTCATCAGATTTTACAGAAGATAAAAGTTGTGGAGACACAGGATGGTGGTAAGAAAGCACCTGAATCGACTCATCAAATTGTAGAACTTGAAGGGATGCTTCAAAAGGAAAGGGAAGACTTTGAGAAATCATTACAAAAAGTGATAAAGGGGGAGGTGAAATCTGGCCAGCCTGCAATTGATATTCTTGAGATCAATAAATTGCGGAGGCAGTTACTCTTCCATTCTTATGTTTGGGACCAGCGCCTGATTCGTGCAGCCAGTCTAAGCAATAATAAATTTCAGGTAGGTTTAACCAGTTCCGTTACCAAACTCAAGGAGAAACCTATTGGCATGGAGACGGTTGTCAAAACACCAGGAAGAAATTTCAGTAGTTGTACAGCTCTTCCCGAAATCAAGCCTGGTATAAATCTTAATCAAGGAGGAGATGCTGGCTACTTTAGCCAGATGGGAGGGGTTCAGAACAGAACTGAAATGGGTCTGGACACAGATCATGGGAATGAGACCTCTGCAAATGTCCTTGATAAATCTGATCCTTTGGAGTCTGAAAAAATTGTACAAATGGGTCTCTCGGAAGAGAATGAATGTTCGGCTGTGGAAAGTTTATCTGATACCCTTGATGCGGCTTGGACTGGTACAACCCACCCAACTAGTACAACATCTAGAGAGAATGGCTATTCACTTCCTCATCCAACGTTGGTAAAATCATCAACTGTGGTAAAAAATGTTGCATCCGCTGTGGAAAATGGTACTGTTGACCAAGGTGGGGTACAGGCAACTCGTTCTCTTATTTCTACATTTCCTGCTGTCACTAGTTCATTCAGTAAAAGTGCTTCCTTTAACACTCAAAAGCTATGTATTGGTGGTGAGCACAGTCCTGTCTATGTTGCACGGTTCCGGGAACTGGAACGGCATACTGGTGCCAGGTTGCTACTGCCGATTGGTGTTAATGATACAGTTATCCCTGTTTTTGATGATGAGCCCACTAGTGTTATTGCATATACTCTCGTCTCTCCTAATTATCATTTGCAGATTTCTGAGCCTGATAGACCAATTGATTCTGCAGTCTCATTACCGCTTTTTGATTCAGCGAATCTTCTCTCACTGAATGCTTTTGATGAGTCAGTTTCTGAGACCTACAGAGGTCTTAGTTCTTCTGATGAGAGCATCTTATCCATGTCACATTCGCGGAGCTCTGAATCTCTTGTGTCAAAGGATATACATGCTAGAGTATCCTTCACAGATGAGGGGCCCCTCGGTAAGGTGAAGTATACAGTGACTTGTTACTACGCATCGCAGTTTGAGGCATTAAGGAAGGCATGTTGCCCTTCCGAATTAGATTTTGTGCGGTCTCTTAGCCGCTGTAAGAAGTGGGGTGCCCAGGGTGGAAAGAGCAACGTTTTCTTTGCTAAGACCTTGGATGATCGGTTTATCATCAAACAAGTCACAAAGACGGAGCTGGAGTCATTTATCAAGTTTGCACCAGCTTATTTCAAATATTTGTCTGATTCAATCAGTTCTAGAAGCCCAACTTGCCTAGCAAAGATCCTGGGTATCTATCAGGTTTCATCAAAACATGGTAAAGCAGGGAAAGAGACAAAGATGGATGTCTTAGTAATGGAGAATCTATTATTTAGACGGAATGTCTCACGGCTCTATGACCTGAAAGGATCTTCTCGATCACGTTATAATCCAGACACAAGTGGAAGCAATAAAGTGCTACTGGACCAGAATCTGATTGAAGCAATGCCTACTTCTCCAATTTTTTTGGGGAACAGGGCAAAGCGGCTATTGGAGAGAGCTGTCTGGAATGATACTGCATTCCTTGCTGCAATCGATGTGATGGACTACTCGTTACTGGTTGGAGTGGATGAGGAAAAGCACGAGCTAGTTTTGGGGATCATTGATTTCATGAGGCAATATACATGGGACAAGCACCTTGAAACTTGGGTGAAGGCCTCAGGCATCCTCGGAGGACCGAAGAATACATCTCCAACCGTGATTTCACCCCAGCAGTATAAGAAACGTTTCAGGAAAGCTATGGCCACTTACTTTCTGATGCTTCCCGATCAGTGGTCGCCTGAGGCGATTGTCCCAAGTGGGTCGCAATCTGACCATTTTGAAGAAACTTCCCAAGCTCAATCTGAAGCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1820

Amino Acids

200.61

Weight (kDa)

5.8

Isoelectric Point (pI)

48.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	3.5e-17	FYVE zinc finger
Cpn60_TCP1	PF00118	417 - 666	1.2e-30	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1563 - 1729	3.3e-34	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1728 - 1784	6.7e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240
fragaria_vesca	FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530
malus_domestica	MD02G1103900.v1.1 MD15G1224300.v1.1
prunus_persica	Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1
pyrus_communis	pycom02g08170 pycom15g19930 pycom15g19940
rosa_chinensis	RchiOBHm_Chr2g0096101
rosa_laevigata	RLG00000016625
rosa_multiflora	Rmu_sc0005996.1_g000006
rosa_roxburghii	Rroxscaffold_2G00145750
rosa_rugosa	Rorug02G0057000 Rorug02G0057100 Rorug02G0057200 Rorug02G0057200
rosa_samantha	Rh2AG104000 Rh2BG106100 Rh2CG108000 Rh2DG106700
rosa_wichuraiana	Rw2G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4995
Acc36I	ACCTGC	2 cut(s)	1204, 1233
AccB1I	GGYRCC	5 cut(s)	5, 1904, 2228, 4226, 4682
AccB7I	CCANNNNNTGG	2 cut(s)	248, 2117
AccI	GTMKAC	1 cut(s)	4577
AccII	CGCG	1 cut(s)	4495
AclI	AACGTT	3 cut(s)	4001, 4704, 5332
AclWI	GGATC	8 cut(s)	1331, 1344, 1468, 2632, 3833, 4846, 4984, 5213
AcoI	YGGCCR	3 cut(s)	184, 3472, 5349
AcsI	RAATTY	5 cut(s)	1793, 3044, 3215, 3584, 3670
AcuI	CTGAAG	4 cut(s)	1580, 1919, 2051, 2640
AcvI	CACGTG	1 cut(s)	469
AdeI	CACNNNGTG	2 cut(s)	821, 2696
AflII	CTTAAG	1 cut(s)	1304
AflIII	ACRYGT	3 cut(s)	1776, 1936, 2690
AgeI	ACCGGT	1 cut(s)	919
AhdI	GACNNNNNGTC	1 cut(s)	4949
AhlI	ACTAGT	3 cut(s)	3956, 4118, 4292
AjuI	GAANNNNNNNTTGG	2 cut(s)	5293, 5325
AloI	GAACNNNNNNTCC	2 cut(s)	547, 579
Alw21I	GWGCWC	6 cut(s)	791, 1152, 1195, 3307, 4179, 4502
AlwI	GGATC	8 cut(s)	1331, 1344, 1468, 2632, 3833, 4846, 4984, 5213
AlwNI	CAGNNNCTG	7 cut(s)	524, 965, 2117, 3020, 3553, 4436, 5041
Ama87I	CYCGRG	1 cut(s)	2290
ApaI	GGGCCC	2 cut(s)	336, 4560
ApoI	RAATTY	5 cut(s)	1793, 3044, 3215, 3584, 3670
ArsI	GACNNNNNNTTYG	4 cut(s)	1502, 1534, 2780, 2812
AseI	ATTAAT	1 cut(s)	864
AsiGI	ACCGGT	1 cut(s)	919
Asp700I	GAANNNNTTC	3 cut(s)	1444, 2640, 5333
AspLEI	GCGC	3 cut(s)	200, 2845, 3552
AsuC2I	CCSGG	2 cut(s)	3272, 4205
AsuII	TTCGAA	1 cut(s)	2737
AsuNHI	GCTAGC	1 cut(s)	2141
AvaI	CYCGRG	1 cut(s)	2290
AvaII	GGWCC	7 cut(s)	455, 1552, 2079, 2973, 3544, 5032, 5282
AxyI	CCTNAGG	3 cut(s)	966, 5266, 5388
BaeGI	GKGCMC	6 cut(s)	8, 336, 474, 1723, 4560, 4687
BalI	TGGCCA	2 cut(s)	3474, 5351
BamHI	GGATCC	1 cut(s)	1336
BanI	GGYRCC	5 cut(s)	5, 1904, 2228, 4226, 4682
BanII	GRGCYC	7 cut(s)	336, 791, 1195, 2728, 4290, 4502, 4560
BarI	GAAGNNNNNNTAC	2 cut(s)	4123, 4155
BauI	CACGAG	1 cut(s)	5189
BbrPI	CACGTG	1 cut(s)	469
BbsI	GAAGAC	3 cut(s)	264, 3116, 3429
Bbv12I	GWGCWC	6 cut(s)	791, 1152, 1195, 3307, 4179, 4502
BceAI	ACGGC	2 cut(s)	4232, 4973
BcgI	CGANNNNNNTGC	4 cut(s)	177, 211, 3354, 3388
BciVI	GTATCC	3 cut(s)	2338, 3234, 4549
BclI	TGATCA	1 cut(s)	2800
BcnI	CCSGG	2 cut(s)	3272, 4205
BcuI	ACTAGT	3 cut(s)	3956, 4118, 4292
BfmI	CTRYAG	7 cut(s)	115, 475, 1068, 2153, 2298, 4368, 4444
BfoI	RGCGCY	1 cut(s)	3553
BfrI	CTTAAG	1 cut(s)	1304
BfuAI	ACCTGC	2 cut(s)	1204, 1233
BfuI	GTATCC	3 cut(s)	2338, 3234, 4549
BglI	GCCNNNNNGGC	1 cut(s)	183
Bme18I	GGWCC	7 cut(s)	455, 1552, 2079, 2973, 3544, 5032, 5282
BmeRI	GACNNNNNGTC	1 cut(s)	4949
BmeT110I	CYCGRG	1 cut(s)	2290
BmrI	ACTGGG	2 cut(s)	136, 1725
BmtI	GCTAGC	1 cut(s)	2145
BmuI	ACTGGG	2 cut(s)	136, 1725
BoxI	GACNNNNGTC	1 cut(s)	3650
BpiI	GAAGAC	3 cut(s)	264, 3116, 3429
BplI	GAGNNNNNCTC	2 cut(s)	2953, 2985
BpmI	CTGGAG	3 cut(s)	2556, 2973, 4787
Bpu14I	TTCGAA	1 cut(s)	2737
BpuEI	CTTGAG	4 cut(s)	1620, 2980, 3515, 3605
BpuMI	CCSGG	2 cut(s)	3272, 4205
Bsa29I	ATCGAT	2 cut(s)	1581, 5142
BsaAI	YACGTR	1 cut(s)	469
BsaBI	GATNNNNATC	1 cut(s)	1587
BsaI	GGTCTC	6 cut(s)	1976, 2781, 3803, 3878, 4433, 4662
BsaWI	WCCGGW	1 cut(s)	919
BsaXI	ACNNNNNCTCC	6 cut(s)	547, 577, 936, 966, 4754, 4784
Bse118I	RCCGGY	1 cut(s)	919
Bse21I	CCTNAGG	3 cut(s)	966, 5266, 5388
Bse3DI	GCAATG	4 cut(s)	169, 305, 2536, 5058
Bse8I	GATNNNNATC	1 cut(s)	1587
BseCI	ATCGAT	2 cut(s)	1581, 5142
BseJI	GATNNNNATC	1 cut(s)	1587
BseMI	GCAATG	4 cut(s)	169, 305, 2536, 5058
BseRI	GAGGAG	6 cut(s)	568, 731, 1160, 1497, 2630, 3744
BseSI	GKGCMC	6 cut(s)	8, 336, 474, 1723, 4560, 4687
BseYI	CCCAGC	1 cut(s)	5316
BsgI	GTGCAG	5 cut(s)	388, 1696, 1964, 3095, 3582
Bsh1236I	CGCG	1 cut(s)	4495
BshNI	GGYRCC	5 cut(s)	5, 1904, 2228, 4226, 4682
BshTI	ACCGGT	1 cut(s)	919
BshVI	ATCGAT	2 cut(s)	1581, 5142
BsiHKAI	GWGCWC	6 cut(s)	791, 1152, 1195, 3307, 4179, 4502
BsiHKCI	CYCGRG	1 cut(s)	2290
BsiSI	CCGG	3 cut(s)	920, 3271, 4204
BslFI	GGGAC	5 cut(s)	104, 1715, 3557, 5249, 5384
BsmBI	CGTCTC	3 cut(s)	594, 3638, 4321
BsmFI	GGGAC	5 cut(s)	104, 1715, 3557, 5249, 5384
BsmI	GAATGC	3 cut(s)	751, 4420, 5126
Bso31I	GGTCTC	6 cut(s)	1976, 2781, 3803, 3878, 4433, 4662
BsoBI	CYCGRG	1 cut(s)	2290
Bsp119I	TTCGAA	1 cut(s)	2737
Bsp120I	GGGCCC	2 cut(s)	332, 4556
Bsp1407I	TGTACA	2 cut(s)	3683, 3862
Bsp19I	CCATGG	1 cut(s)	576
BspDI	ATCGAT	2 cut(s)	1581, 5142
BspFNI	CGCG	1 cut(s)	4495
BspHI	TCATGA	1 cut(s)	5217
BspMAI	CTGCAG	2 cut(s)	2157, 4372
BspMI	ACCTGC	2 cut(s)	1204, 1233
BspOI	GCTAGC	1 cut(s)	2145
BspPI	GGATC	8 cut(s)	1331, 1344, 1468, 2632, 3833, 4846, 4984, 5213
BspQI	GCTCTTC	2 cut(s)	3312, 3696
BspT104I	TTCGAA	1 cut(s)	2737
BspT107I	GGYRCC	5 cut(s)	5, 1904, 2228, 4226, 4682
BspTI	CTTAAG	1 cut(s)	1304
BspTNI	GGTCTC	6 cut(s)	1976, 2781, 3803, 3878, 4433, 4662
BsrDI	GCAATG	4 cut(s)	169, 305, 2536, 5058
BsrFI	RCCGGY	1 cut(s)	919
BsrGI	TGTACA	2 cut(s)	3683, 3862
BssAI	RCCGGY	1 cut(s)	919
BssNAI	GTATAC	1 cut(s)	4578
BssSI	CACGAG	1 cut(s)	5189
BssT1I	CCWWGG	5 cut(s)	576, 1477, 2442, 4066, 4722
Bst1107I	GTATAC	1 cut(s)	4578
Bst2BI	CACGAG	1 cut(s)	5189
Bst6I	CTCTTC	5 cut(s)	2631, 3312, 3530, 3696, 3876
BstAFI	CTTAAG	1 cut(s)	1304
BstAPI	GCANNNNNTGC	1 cut(s)	5132
BstAUI	TGTACA	2 cut(s)	3683, 3862
BstBAI	YACGTR	1 cut(s)	469
BstBI	TTCGAA	1 cut(s)	2737
BstDSI	CCRYGG	1 cut(s)	576
BstEII	GGTNACC	1 cut(s)	2807
BstENI	CCTNNNNNAGG	1 cut(s)	4565
BstFNI	CGCG	1 cut(s)	4495
BstH2I	RGCGCY	1 cut(s)	3553
BstHHI	GCGC	3 cut(s)	200, 2845, 3552
BstNSI	RCATGY	4 cut(s)	1780, 1940, 4532, 4629
BstPAI	GACNNNNGTC	1 cut(s)	3650
BstPI	GGTNACC	1 cut(s)	2807
BstSFI	CTRYAG	7 cut(s)	115, 475, 1068, 2153, 2298, 4368, 4444
BstSLI	GKGCMC	6 cut(s)	8, 336, 474, 1723, 4560, 4687
BstUI	CGCG	1 cut(s)	4495
BstV2I	GAAGAC	3 cut(s)	264, 3116, 3429
BstX2I	RGATCY	4 cut(s)	1336, 2624, 4851, 4976
BstYI	RGATCY	4 cut(s)	1336, 2624, 4851, 4976
BstZ17I	GTATAC	1 cut(s)	4578
Bsu15I	ATCGAT	2 cut(s)	1581, 5142
Bsu36I	CCTNAGG	3 cut(s)	966, 5266, 5388
BsuI	GTATCC	3 cut(s)	2338, 3234, 4549
BsuTUI	ATCGAT	2 cut(s)	1581, 5142
BtgI	CCRYGG	1 cut(s)	576
BtgZI	GCGATG	1 cut(s)	4584
BtsI	GCAGTG	3 cut(s)	570, 1819, 2152
BtsIMutI	CAGTG	9 cut(s)	426, 570, 1713, 1819, 2152, 2983, 4409, 4587, 5384
BveI	ACCTGC	2 cut(s)	1204, 1233
CaiI	CAGNNNCTG	7 cut(s)	524, 965, 2117, 3020, 3553, 4436, 5041
CciI	TCATGA	1 cut(s)	5217
CfoI	GCGC	3 cut(s)	200, 2845, 3552
Cfr10I	RCCGGY	1 cut(s)	919
ClaI	ATCGAT	2 cut(s)	1581, 5142
CsiI	ACCWGGT	1 cut(s)	2095
CspAI	ACCGGT	1 cut(s)	919
CspCI	CAANNNNNGTGG	2 cut(s)	1452, 1487
DraI	TTTAAA	1 cut(s)	2217
DraIII	CACNNNGTG	2 cut(s)	821, 2696
DriI	GACNNNNNGTC	1 cut(s)	4949
EaeI	YGGCCR	3 cut(s)	184, 3472, 5349
Eam1104I	CTCTTC	5 cut(s)	2631, 3312, 3530, 3696, 3876
Eam1105I	GACNNNNNGTC	1 cut(s)	4949
EarI	CTCTTC	5 cut(s)	2631, 3312, 3530, 3696, 3876
EciI	GGCGGA	1 cut(s)	3290
Ecl136II	GAGCTC	3 cut(s)	789, 1193, 4500
Eco130I	CCWWGG	5 cut(s)	576, 1477, 2442, 4066, 4722
Eco147I	AGGCCT	2 cut(s)	2172, 5264
Eco24I	GRGCYC	7 cut(s)	336, 791, 1195, 2728, 4290, 4502, 4560
Eco31I	GGTCTC	6 cut(s)	1976, 2781, 3803, 3878, 4433, 4662
Eco32I	GATATC	1 cut(s)	1085
Eco47I	GGWCC	7 cut(s)	455, 1552, 2079, 2973, 3544, 5032, 5282
Eco53kI	GAGCTC	3 cut(s)	789, 1193, 4500
Eco57I	CTGAAG	4 cut(s)	1580, 1919, 2051, 2640
Eco72I	CACGTG	1 cut(s)	469
Eco81I	CCTNAGG	3 cut(s)	966, 5266, 5388
Eco88I	CYCGRG	1 cut(s)	2290
Eco91I	GGTNACC	1 cut(s)	2807
EcoICRI	GAGCTC	3 cut(s)	789, 1193, 4500
EcoNI	CCTNNNNNAGG	1 cut(s)	4565
EcoO109I	RGGNCCY	3 cut(s)	333, 4556, 4557
EcoO65I	GGTNACC	1 cut(s)	2807
EcoRV	GATATC	1 cut(s)	1085
EcoT14I	CCWWGG	5 cut(s)	576, 1477, 2442, 4066, 4722
EcoT22I	ATGCAT	1 cut(s)	1367
EcoT38I	GRGCYC	7 cut(s)	336, 791, 1195, 2728, 4290, 4502, 4560
ErhI	CCWWGG	5 cut(s)	576, 1477, 2442, 4066, 4722
Esp3I	CGTCTC	3 cut(s)	594, 3638, 4321
FalI	AAGNNNNNCTT	8 cut(s)	1629, 1661, 2862, 2894, 3390, 3422, 4822, 4854
FaqI	GGGAC	5 cut(s)	104, 1715, 3557, 5249, 5384
FauNDI	CATATG	1 cut(s)	892
FbaI	TGATCA	1 cut(s)	2800
FblI	GTMKAC	1 cut(s)	4577
FriOI	GRGCYC	7 cut(s)	336, 791, 1195, 2728, 4290, 4502, 4560
GlaI	GCGC	3 cut(s)	199, 2844, 3551
GsaI	CCCAGC	1 cut(s)	5320
GsuI	CTGGAG	3 cut(s)	2556, 2973, 4787
HaeII	RGCGCY	1 cut(s)	3553
HapII	CCGG	3 cut(s)	920, 3271, 4204
HhaI	GCGC	3 cut(s)	200, 2845, 3552
Hin6I	GCGC	3 cut(s)	198, 2843, 3550
HinP1I	GCGC	3 cut(s)	198, 2843, 3550
HincII	GTYRAC	2 cut(s)	37, 4063
HindII	GTYRAC	2 cut(s)	37, 4063
HindIII	AAGCTT	3 cut(s)	379, 2454, 2755
HpaII	CCGG	3 cut(s)	920, 3271, 4204
Hpy166II	GTNNAC	9 cut(s)	37, 147, 207, 2108, 2150, 2464, 2720, 4063, 4578
Hpy8I	GTNNAC	9 cut(s)	37, 147, 207, 2108, 2150, 2464, 2720, 4063, 4578
Hpy99I	CGWCG	3 cut(s)	1019, 1440, 2068
HpyCH4IV	ACGT	9 cut(s)	468, 1274, 1425, 1465, 2692, 4001, 4704, 4990, 5332
HpySE526I	ACGT	9 cut(s)	468, 1274, 1425, 1465, 2692, 4001, 4704, 4990, 5332
HspAI	GCGC	3 cut(s)	198, 2843, 3550
Ksp22I	TGATCA	1 cut(s)	2800
LguI	GCTCTTC	2 cut(s)	3312, 3696
MabI	ACCWGGT	1 cut(s)	2095
MaeII	ACGT	9 cut(s)	468, 1274, 1425, 1465, 2692, 4001, 4704, 4990, 5332
MfeI	CAATTG	3 cut(s)	1140, 3483, 4359
MflI	RGATCY	4 cut(s)	1336, 2624, 4851, 4976
MlsI	TGGCCA	2 cut(s)	3474, 5351
MluNI	TGGCCA	2 cut(s)	3474, 5351
MmeI	TCCRAC	5 cut(s)	1242, 1361, 4022, 5149, 5324
Mox20I	TGGCCA	2 cut(s)	3474, 5351
Mph1103I	ATGCAT	1 cut(s)	1367
MroXI	GAANNNNTTC	3 cut(s)	1444, 2640, 5333
MscI	TGGCCA	2 cut(s)	3474, 5351
MslI	CAYNNNNRTG	9 cut(s)	575, 687, 756, 1596, 1812, 2517, 3534, 3804, 4224
Msp20I	TGGCCA	2 cut(s)	3474, 5351
MspA1I	CMGCKG	5 cut(s)	114, 2117, 3077, 4043, 4668
MspCI	CTTAAG	1 cut(s)	1304
MspI	CCGG	3 cut(s)	920, 3271, 4204
MunI	CAATTG	3 cut(s)	1140, 3483, 4359
Mva1269I	GAATGC	3 cut(s)	751, 4420, 5126
MvnI	CGCG	1 cut(s)	4495
NciI	CCSGG	2 cut(s)	3272, 4205
NcoI	CCATGG	1 cut(s)	576
NdeI	CATATG	1 cut(s)	892
NheI	GCTAGC	1 cut(s)	2141
NmeAIII	GCCGAG	1 cut(s)	212
NmuCI	GTSAC	4 cut(s)	4114, 4485, 4582, 4750
NsiI	ATGCAT	1 cut(s)	1367
NspI	RCATGY	4 cut(s)	1780, 1940, 4532, 4629
NspV	TTCGAA	1 cut(s)	2737
PagI	TCATGA	1 cut(s)	5217
PasI	CCCWGGG	1 cut(s)	4687
PceI	AGGCCT	2 cut(s)	2172, 5264
PciI	ACATGT	2 cut(s)	1776, 1936
PciSI	GCTCTTC	2 cut(s)	3312, 3696
PcsI	WCGNNNNNNNCGW	1 cut(s)	5390
PctI	GAATGC	3 cut(s)	751, 4420, 5126
PdmI	GAANNNNTTC	3 cut(s)	1444, 2640, 5333
PflMI	CCANNNNNTGG	2 cut(s)	248, 2117
PfoI	TCCNGGA	1 cut(s)	4203
PinAI	ACCGGT	1 cut(s)	919
PmaCI	CACGTG	1 cut(s)	469
PmlI	CACGTG	1 cut(s)	469
Ppu21I	YACGTR	1 cut(s)	469
PscI	ACATGT	2 cut(s)	1776, 1936
PshAI	GACNNNNGTC	1 cut(s)	3650
PshBI	ATTAAT	1 cut(s)	864
PsiI	TTATAA	1 cut(s)	4995
Psp124BI	GAGCTC	3 cut(s)	791, 1195, 4502
Psp1406I	AACGTT	3 cut(s)	4001, 4704, 5332
PspCI	CACGTG	1 cut(s)	469
PspEI	GGTNACC	1 cut(s)	2807
PspFI	CCCAGC	1 cut(s)	5316
PspOMI	GGGCCC	2 cut(s)	332, 4556
PstI	CTGCAG	2 cut(s)	2157, 4372
PstNI	CAGNNNCTG	7 cut(s)	524, 965, 2117, 3020, 3553, 4436, 5041
PsuI	RGATCY	4 cut(s)	1336, 2624, 4851, 4976
PvuII	CAGCTG	3 cut(s)	114, 2117, 3077
RseI	CAYNNNNRTG	9 cut(s)	575, 687, 756, 1596, 1812, 2517, 3534, 3804, 4224
SacI	GAGCTC	3 cut(s)	791, 1195, 4502
SapI	GCTCTTC	2 cut(s)	3312, 3696
SexAI	ACCWGGT	1 cut(s)	2095
SfcI	CTRYAG	7 cut(s)	115, 475, 1068, 2153, 2298, 4368, 4444
SfuI	TTCGAA	1 cut(s)	2737
SinI	GGWCC	7 cut(s)	455, 1552, 2079, 2973, 3544, 5032, 5282
SmiMI	CAYNNNNRTG	9 cut(s)	575, 687, 756, 1596, 1812, 2517, 3534, 3804, 4224
SmlI	CTYRAG	5 cut(s)	1304, 1635, 2959, 3494, 3620
SmoI	CTYRAG	5 cut(s)	1304, 1635, 2959, 3494, 3620
SpeI	ACTAGT	3 cut(s)	3956, 4118, 4292
SseBI	AGGCCT	2 cut(s)	2172, 5264
SspI	AATATT	2 cut(s)	1678, 4805
SstI	GAGCTC	3 cut(s)	791, 1195, 4502
StuI	AGGCCT	2 cut(s)	2172, 5264
StyI	CCWWGG	5 cut(s)	576, 1477, 2442, 4066, 4722
TaiI	ACGT	9 cut(s)	471, 1277, 1428, 1468, 2695, 4004, 4707, 4993, 5335
TaqII	GACCGA	1 cut(s)	5299
TatI	WGTACW	4 cut(s)	206, 3683, 3862, 3959
TauI	GCSGC	4 cut(s)	177, 3932, 4668, 5095
TscAI	CASTG	9 cut(s)	433, 577, 1720, 1819, 2152, 2983, 4416, 4587, 5384
TseFI	GTSAC	4 cut(s)	4114, 4485, 4582, 4750
Tsp45I	GTSAC	4 cut(s)	4114, 4485, 4582, 4750
TspGWI	ACGGA	3 cut(s)	3598, 4775, 4960
TspRI	CASTG	9 cut(s)	433, 577, 1720, 1819, 2152, 2983, 4416, 4587, 5384
Van91I	CCANNNNNTGG	2 cut(s)	248, 2117
Vha464I	CTTAAG	1 cut(s)	1304
VpaK11BI	GGWCC	7 cut(s)	455, 1552, 2079, 2973, 3544, 5032, 5282
VspI	ATTAAT	1 cut(s)	864
XagI	CCTNNNNNAGG	1 cut(s)	4565
XapI	RAATTY	5 cut(s)	1793, 3044, 3215, 3584, 3670
XbaI	TCTAGA	2 cut(s)	3968, 4826
XceI	RCATGY	4 cut(s)	1780, 1940, 4532, 4629
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1168
XmiI	GTMKAC	1 cut(s)	4577
XmnI	GAANNNNTTC	3 cut(s)	1444, 2640, 5333
Zsp2I	ATGCAT	1 cut(s)	1367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.