Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002065

Physical Location & Seq

Genomic Coordinates Forward (+)

8338439 .. 8346808

Gene Structure 8370 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016625

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5463 bp

ATGGGCACCCCTGACAACAAATTATCAGAGATAGTTGACATTTTCAAGTCTTGGATGCCCAGGAGAACCGAGCCAACCAATGTGTCCAGGGACTTTTGGATGCCCGATCACAGCTGTAGGGTATGCTACGACTGTGATTCCCAGTTCACAGTATTTAATCGAAGGCATCATTGTCGCCTTTGTGGCCGTGTGTTCTGCGCTAGGTGTACTGCAAACTCTATTCCTCCCCCATCTGATGAGCCAAGAATTGGTAGGGAAGACGGGGAGAGGATTAGGGTGTGTAATTTTTGCTTCAAGCAATGGGAGCAAGGGATAGCAGCAGTTGATAATGGGCCCCCTGAACCTAGCCCTGGCCTCAGTCCATCGCCATCTACAACAAGCTTGGTCAGTACCAACACAAGCTGCACCTACCACAGTAGTAGCAGCACTGTTGGTTCTACCCCTTATTCGACTGGACCTTACCAGCACGTGCCCTATAGTTCTGGTCTTAGCCCCAGACAATCTTGCCAAGATGATTCAGTTACTGGCCTGCAAGACAATGTAACATCTCAGAGGAGCATTAGTTCTGACGCAGCCATGGCAGAGTCATCTCCCAACCAGTTTGGCTTTTGCATGGACAGGAGTGATGATGAGGATGATGACTATGGTTATCATTCAGATTCAGAATCAAGACATTTCACCCATGCCAATGACTATTATGGTGCAATCAATAACGAGGAGTTTGATAGCGTGTATGAACCACAGAAGGTGCATTCTGATGGAGAAACCACAGATGCAAAAAGTTTGAGCTCCTTTTCACCTGAGAAGTTTGATACACAGGGTGTTGTGGGAACGAAACTTGGGGAAGAATCACATTATCATATTAATGGTGATGAATGTGAAACTTCTCCATATGAGATGGAGACTAAAAATGCAGAACCGGTGGATTTTGAGAATAATGGGCTACTTTGGCTCCCACCAGAACCTGAGGATGAAGAAGATGACAGAGAAGCTGACTTATTTGATGATGATGATGATGATGAGGGTGGTGATGGCGGTGGTGGTGGTCGTGGTGGTCGTGGTCGTGGTGTTGGTGGTGCTACAGGGGAGTGGGGATATCTTCATTCTTCCAACAGTTTTGGTGGTGGGGAGTGTCGTACTAGGGAGAAGTCAATTGAGGAGCACAGGAAAGCCATGAAGAATGTGGTGGAAGGTCATTTTAGAGCTCTGGTATCACAGCTCTTGCAGGTTGAAAACCTGCCTTCGATTGATGAAAATTACAAAGAGACTTGGTTGGACATAATTACATCTTTGTCATGGGAAGCTGCCACACTTCTTAAGCCAGATACGAGCAAAGGGGGAGGAATGGATCCTGGGGGATATGTGAAGGTTAAATGCATAGCTTGTGGGAGTCGGAGCGAGAGCATGGTGGTTAAAGGAGTTGTTTGCAAGAAAAATGTGGCTCATCGGCGAATGACTTCAAAGATAGAAAAACCACGTTTCTTGATCCTTGGAGGAGCTTTGGAATATCAGCGTGTATCAAACCACTTGTCCAGTTTCGATACTTTGTTGCAGCAGGAGATGGACCATCTGAAGATGGCAGTTGCCAAGATCGACGCACATCACCCTAATGTTCTATTGGTGGAGAAGTCTGTATCTCGGTATGCTCAAGATTACCTTCTTGCGAAAGACATATCACTTGTTCTGAATATTAAGAGGCCACTTTTGGAGCGAATATCTCGCTGCACTGGGGCACAAATTGTCCCTTCGATAGATCATCTTACATCACCAAAACTAGGGTATTGCGACATGTTCCATGTGGAGAAATTTTTTGAAGTGCATGGCAGTGCTGGACAAGGTGGGAAGAAATTAACAAAGACTCTGATGTTCTTTGAAGGTTGTCCAAAGCCATTGGGTGTTACTATATTGCTGAAGGGTGCCAATGGGGATGAGTTAAAAAAGGTGAAACATGTTGTGCAGTATGGAGTTTTTGCAGCTTATCACTTGGCTTTGGAGACCTCATTTTTAGCTGATGAAGGAGCCTCCCTTCCAGAACTTCCTCTGAAGTCCGTGGTAACTGTATTGCCTGATAAACCATCGAGTATTGACAGGTCCATTTCCACCATACCTGGTTTTAGTGTACCAGCAGTTGGCAAGCCTCAGAGTTCCAATGCTAGCAGTGAACTGCAGAACTCAAATAAAGGCCTCATTTCAGATAGTGGCTCATTTGCCCAAGTTGCCTCCATTTTAAAGAACGAAGGCACCAATTTGGTCTGCTTGCCTAAAGCTCCATGTTCTCAACCTTCCCCCATAAAACATACCCCGAGTCCTATAGAATATACATCTTCTTTCACTTCCTTGTCTCCCCCAGGACAGGATACGATAGACTCCTGCCATAAAGAGCTTTCTTCAATTTGTGGCTCTGACGACAACAAGGATGTAAGTTCTAAAGAATCTTGTCCAGCCAAAACAGCCAAGGCAGGCGAAGCTTTAGTCCACGATAGTTTGATCTCTAATTCTTTTAGCACCTCAGAGGCATTTGGACATGGTAGTGGCGTTGCTTTAGCTGCAAATCTCAGAGATACTCCAGAACTTCCATCCATAAAATATCATACTGATAATCAGAATGAGGAGGTGGGATCTTCAAAAGAAGAGTTTCCCCCCTCACCTTCAGACCACCAGAGCATTTTGGTTTCCCTATCAACACGTTGTGTGTGGAAAGGAACTGTATGTGAACGGGCCCATCTTTTTCGAATCAAATACTATGGAAGCTTTGATAAGCCTCTGGGGAGGTTTTTACGAGACCATCTGTTTGATCAGGATTACCTCTGTCGTTCATGTGGGATGCCCTCAGAAGCGCATATTCATTGTTATACTCATAGACAAGGAAGCCTTACAATATCTGTTAAGAAACTCCCAGAGATTTTCCTGCCAGGGGAAAAGGAAGGAAAAATATGGATGTGGCATAGGTGCTTGAGATGTCCTCGGACCAGTGGCTTCCCTCCAGCCACTCGTAGAGTTGTAATGTCAGATGCTGCTTGGGGTTTATCTTTTGGGAAATTTCTGGAATTGAGCTTTTCAAATCATGCAGCTGCAAACAGAGTTGCAAGCTGTGGTCATTCCCTACACAGAGATTGTCTTCGGTTTTATGGGTTTGGGAGAATGGTTGCTTGCTTCCGCTATGCCTCGATTCATATTCACTCTGTTTGTCTGCCCCCACCAAAACTTGAATTTTACTATGATAATCAGGAATGGATACAAAAAGAAGCATATGAGGTGGGCAACCGGGCGGAGCTTCTATTTACTGAATTACATAGTGCTCTTCATCAGATTTTACAGAAGATAAAAGTTGCGGAGACACAGGATGGTGGTAAGAAAGCACCTGAATCGACTCATCAAATTGTAGAACTTGAAGGGATGCTTCAAAAGGAAAGGGAAGACTTTGAGAAGTCGTTACAAAAAGTGATAAAGGGGGAGGTGAAATCTGGCCAGCCTGCAATTGATATTCTTGAGATCAATAAATTGCGGAGGCAGTTACTCTTCCACTCTTATATTTTGGACCAGCGCCTGATTCGTGCAGCCAGTCTAAGCAATAATAAATTTCAGGAAGGTTTAACCAGTTCCGTTACCAAACTCAAGGAGAAACCTATTGGCATGGAGAAGGTTGTCAAAACACCAGGAAGAAATTTCAGTAGTTGTACAGCTCTTCCCGAAATCAAGCCTGGTATGAATCTTAATCAAGGAGGAGATGCTGGCTACTTTAGCCATATGGGAGGGGTTCAGAACAGAACTGAAATGGGTCTGGACACAGATCATGGGAATGAGACCTCTGCAGATGTCCTTGATAAATCTGATCCTTTGGAGTCTGAAAAAATTGTACAAATGGGTCTCTCGGAAGAGAGTGAATGTTCGGCTGTGGAAAGTTTATCTGATACCCTTGATGCGGCTTGGACTGGTACAACCCACCCAACTAGTACAACATCTAGAGAGAATGGCTATTCACTTCCTCATCAAACCTTGGTAAAATCATCAACTCTGGTAAAAAATGTTGCATCCGCTGTGGAAAATGGTACTGTTGACCAAGGCGGGGTACAGGCAACTCGTTCTCTTATTTCTACATTTCCTGCTGTCACTAGTTCATTCAGTAAAAGTGCTTCCTTTAACACTCAAAAGCTATGTATTGGTGAGCACAGTCCTGTCTATGTTGCACGGTTCCGGGAACTGGAACGGCATACTGGTGCCAGGTTGCTACTGCCGATTGGTGTTAATGATACAGTTATCCCTGTTTTTGATGATGAGCCCACTAGTGTTATTGCATATACCCTCGTCTCTCCTAATTATCATTTGCAGATTTCTGAGCCGGATAGATCAAAAGAAGCTATTGATTCTGCAGTCTCATTACCGCTTTTTGATTCAGCGAATCTTCTCTCAATGAATGCTTTTGATGAGTCAGTTTCTGAGACCTACAGAGGTCTTAGTTCTTCTGATGAGAGCATCTTATCCATGTCACGTTCACGCAGCTCTGAATCTCTTGTGTCAAAGGATATACATGCTAGAGTATCCTTTACAGATGAGGGGCCCCTCGGTAAGGTGAAGTATACAGTGACTTGTTACTATGCATCGCAGTTTGAGGCATTAAGGAAGGCATGTTGCCCTTCTGAATTAGATTTTGTGCGGTCTCTTAGCCGCTGTAAGAAGTGGGGTGCCCAGGGTGGAAAGAGCAACGTTTTCTTTGCTAAAACCTTGGATGATCGGTTTATCATCAAACAAGTCACAAAGACGGAGCTGGAGTCATTTATCAAGTTTGCACCAGCTTATTTCAAATATTTGTCTGATTCAATCAGTACTAGAAGCCCAACTTGCCTAGCAAAGATCCTGGGTATCTATCAGGTTTCATCAAAGCATGGTAAAGCAGGGAAAGAGACAAAGATGGATGTCTTAGTAATGGAGAATCTATTATTTAGACGGAATGTCTCACGGCTCTATGACCTGAAAGGATCTTCTCGATCACGTTATAATCCAGATACAAGTGGGAGCAATAAAGTGCTACTGGACCAGAATCTGATTGAAGCAATGCCTACTTCACCAATTTTTGTGGGGAACAGGGCAAAGCGGCTATTGGAGAGAGCTGTCTGGAATGATACTGCATTCCTTGCTTCAATCGATGTGATGGACTACTCATTACTGGTGGGAGTGGATGAGGAAAAGCACGAGCTAGTTTTGGGGATCATTGATTTCATGAGGCAATATACATGGGACAAGCACCTTGAAACTTGGGTGAAGGCCTCAGGCATCCTCGGAGGACCAAAGAATACATCTCCAACCGTGATTTCACCCCAGCAGTATAAGAAACGTTTCAGGAAAGCTATGGCCACCTACTTTCTGATGCTTCCCGATCAGTGGTCCCCTGAGGCGATTGTCCCAAGTGGGTCGCAATCTGACCATTTTGAAGAAACTTCCCAAGCTCAATCTGAAGCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1821

Amino Acids

201.07

Weight (kDa)

5.92

Isoelectric Point (pI)

48.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	2.4e-17	FYVE zinc finger
Cpn60_TCP1	PF00118	421 - 670	1.2e-30	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1563 - 1729	2.1e-34	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1728 - 1784	6.7e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240
fragaria_vesca	FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530
malus_domestica	MD02G1103900.v1.1 MD15G1224300.v1.1
prunus_persica	Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1
pyrus_communis	pycom02g08170 pycom15g19930 pycom15g19940
rosa_chinensis	RchiOBHm_Chr2g0096101
rosa_laevigata	RLG00000016625
rosa_multiflora	Rmu_sc0005996.1_g000006
rosa_roxburghii	Rroxscaffold_2G00145750
rosa_rugosa	Rorug02G0057000 Rorug02G0057100 Rorug02G0057200 Rorug02G0057200
rosa_samantha	Rh2AG104000 Rh2BG106100 Rh2CG108000 Rh2DG106700
rosa_wichuraiana	Rw2G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4995
Acc36I	ACCTGC	2 cut(s)	1216, 1245
AccB1I	GGYRCC	5 cut(s)	5, 1916, 2240, 4217, 4682
AccB7I	CCANNNNNTGG	2 cut(s)	248, 2129
AccI	GTMKAC	1 cut(s)	4577
AclI	AACGTT	2 cut(s)	4704, 5332
AclWI	GGATC	8 cut(s)	1343, 1356, 1480, 2626, 3827, 4846, 4984, 5213
AcoI	YGGCCR	3 cut(s)	184, 3466, 5349
AcsI	RAATTY	5 cut(s)	1805, 3038, 3209, 3578, 3664
AcuI	CTGAAG	4 cut(s)	1592, 1931, 2063, 2634
AcvI	CACGTG	1 cut(s)	469
AdeI	CACNNNGTG	2 cut(s)	821, 2690
AflII	CTTAAG	1 cut(s)	1316
AflIII	ACRYGT	3 cut(s)	1788, 1948, 2684
AgeI	ACCGGT	1 cut(s)	919
AhdI	GACNNNNNGTC	1 cut(s)	4949
AhlI	ACTAGT	3 cut(s)	3950, 4112, 4283
AjuI	GAANNNNNNNTTGG	2 cut(s)	5293, 5325
AloI	GAACNNNNNNTCC	2 cut(s)	547, 579
Alw21I	GWGCWC	5 cut(s)	791, 1164, 1207, 3301, 4170
AlwI	GGATC	8 cut(s)	1343, 1356, 1480, 2626, 3827, 4846, 4984, 5213
AlwNI	CAGNNNCTG	6 cut(s)	524, 965, 3014, 3547, 4436, 5041
Ama87I	CYCGRG	1 cut(s)	2302
ApaI	GGGCCC	3 cut(s)	336, 2722, 4560
ApoI	RAATTY	5 cut(s)	1805, 3038, 3209, 3578, 3664
ArsI	GACNNNNNNTTYG	6 cut(s)	1514, 1546, 2457, 2489, 2774, 2806
AseI	ATTAAT	1 cut(s)	864
AsiGI	ACCGGT	1 cut(s)	919
Asp700I	GAANNNNTTC	3 cut(s)	1456, 2634, 5333
AspLEI	GCGC	3 cut(s)	200, 2839, 3546
AsuC2I	CCSGG	2 cut(s)	3266, 4196
AsuII	TTCGAA	1 cut(s)	2731
AsuNHI	GCTAGC	1 cut(s)	2153
AvaI	CYCGRG	1 cut(s)	2302
AvaII	GGWCC	8 cut(s)	455, 1564, 2091, 2967, 3538, 5032, 5282, 5382
AxyI	CCTNAGG	3 cut(s)	966, 5266, 5388
BaeGI	GKGCMC	7 cut(s)	8, 336, 474, 1735, 2722, 4560, 4687
BalI	TGGCCA	2 cut(s)	3468, 5351
BamHI	GGATCC	1 cut(s)	1348
BanI	GGYRCC	5 cut(s)	5, 1916, 2240, 4217, 4682
BanII	GRGCYC	6 cut(s)	336, 791, 1207, 2722, 4281, 4560
BarI	GAAGNNNNNNTAC	2 cut(s)	4117, 4149
BauI	CACGAG	1 cut(s)	5189
BbrPI	CACGTG	1 cut(s)	469
BbsI	GAAGAC	3 cut(s)	264, 3110, 3423
Bbv12I	GWGCWC	5 cut(s)	791, 1164, 1207, 3301, 4170
BceAI	ACGGC	3 cut(s)	171, 4223, 4973
BcgI	CGANNNNNNTGC	4 cut(s)	155, 189, 3348, 3382
BciVI	GTATCC	3 cut(s)	2350, 3228, 4549
BclI	TGATCA	1 cut(s)	2794
BcnI	CCSGG	2 cut(s)	3266, 4196
BcuI	ACTAGT	3 cut(s)	3950, 4112, 4283
BfmI	CTRYAG	8 cut(s)	115, 475, 1080, 2165, 2310, 3810, 4368, 4444
BfoI	RGCGCY	1 cut(s)	3547
BfrI	CTTAAG	1 cut(s)	1316
BfuAI	ACCTGC	2 cut(s)	1216, 1245
BfuI	GTATCC	3 cut(s)	2350, 3228, 4549
BglI	GCCNNNNNGGC	1 cut(s)	183
BmcAI	AGTACT	1 cut(s)	4825
Bme18I	GGWCC	8 cut(s)	455, 1564, 2091, 2967, 3538, 5032, 5282, 5382
BmeRI	GACNNNNNGTC	1 cut(s)	4949
BmeT110I	CYCGRG	1 cut(s)	2302
BmrI	ACTGGG	2 cut(s)	136, 1737
BmtI	GCTAGC	1 cut(s)	2157
BmuI	ACTGGG	2 cut(s)	136, 1737
BpiI	GAAGAC	3 cut(s)	264, 3110, 3423
BplI	GAGNNNNNCTC	2 cut(s)	2947, 2979
BpmI	CTGGAG	3 cut(s)	2550, 2967, 4787
Bpu14I	TTCGAA	1 cut(s)	2731
BpuEI	CTTGAG	4 cut(s)	1632, 2974, 3509, 3599
BpuMI	CCSGG	2 cut(s)	3266, 4196
Bsa29I	ATCGAT	1 cut(s)	5142
BsaAI	YACGTR	1 cut(s)	469
BsaI	GGTCTC	6 cut(s)	1988, 2775, 3797, 3872, 4433, 4662
BsaWI	WCCGGW	1 cut(s)	919
BsaXI	ACNNNNNCTCC	6 cut(s)	547, 577, 936, 966, 4754, 4784
Bse118I	RCCGGY	1 cut(s)	919
Bse21I	CCTNAGG	3 cut(s)	966, 5266, 5388
Bse3DI	GCAATG	2 cut(s)	305, 5058
BseCI	ATCGAT	1 cut(s)	5142
BseMI	GCAATG	2 cut(s)	305, 5058
BseRI	GAGGAG	6 cut(s)	568, 731, 1172, 1509, 2624, 3738
BseSI	GKGCMC	7 cut(s)	8, 336, 474, 1735, 2722, 4560, 4687
BseYI	CCCAGC	1 cut(s)	5316
BsgI	GTGCAG	4 cut(s)	388, 1708, 1976, 3576
BshNI	GGYRCC	5 cut(s)	5, 1916, 2240, 4217, 4682
BshTI	ACCGGT	1 cut(s)	919
BshVI	ATCGAT	1 cut(s)	5142
BsiHKAI	GWGCWC	5 cut(s)	791, 1164, 1207, 3301, 4170
BsiHKCI	CYCGRG	1 cut(s)	2302
BsiSI	CCGG	4 cut(s)	920, 3265, 4195, 4340
BslFI	GGGAC	5 cut(s)	104, 1727, 5249, 5368, 5384
BsmBI	CGTCTC	1 cut(s)	4312
BsmFI	GGGAC	5 cut(s)	104, 1727, 5249, 5368, 5384
BsmI	GAATGC	3 cut(s)	751, 4420, 5126
Bso31I	GGTCTC	6 cut(s)	1988, 2775, 3797, 3872, 4433, 4662
BsoBI	CYCGRG	1 cut(s)	2302
Bsp119I	TTCGAA	1 cut(s)	2731
Bsp120I	GGGCCC	3 cut(s)	332, 2718, 4556
Bsp1407I	TGTACA	2 cut(s)	3677, 3856
Bsp19I	CCATGG	1 cut(s)	576
BspDI	ATCGAT	1 cut(s)	5142
BspHI	TCATGA	1 cut(s)	5217
BspMAI	CTGCAG	3 cut(s)	2169, 3814, 4372
BspMI	ACCTGC	2 cut(s)	1216, 1245
BspOI	GCTAGC	1 cut(s)	2157
BspPI	GGATC	8 cut(s)	1343, 1356, 1480, 2626, 3827, 4846, 4984, 5213
BspQI	GCTCTTC	2 cut(s)	3306, 3690
BspT104I	TTCGAA	1 cut(s)	2731
BspT107I	GGYRCC	5 cut(s)	5, 1916, 2240, 4217, 4682
BspTI	CTTAAG	1 cut(s)	1316
BspTNI	GGTCTC	6 cut(s)	1988, 2775, 3797, 3872, 4433, 4662
BsrDI	GCAATG	2 cut(s)	305, 5058
BsrFI	RCCGGY	1 cut(s)	919
BsrGI	TGTACA	2 cut(s)	3677, 3856
BssAI	RCCGGY	1 cut(s)	919
BssNAI	GTATAC	1 cut(s)	4578
BssSI	CACGAG	1 cut(s)	5189
BssT1I	CCWWGG	6 cut(s)	576, 1489, 2454, 3996, 4060, 4722
Bst1107I	GTATAC	1 cut(s)	4578
Bst2BI	CACGAG	1 cut(s)	5189
Bst6I	CTCTTC	5 cut(s)	2625, 3306, 3524, 3690, 3870
BstAFI	CTTAAG	1 cut(s)	1316
BstAPI	GCANNNNNTGC	1 cut(s)	5132
BstAUI	TGTACA	2 cut(s)	3677, 3856
BstBAI	YACGTR	1 cut(s)	469
BstBI	TTCGAA	1 cut(s)	2731
BstDSI	CCRYGG	2 cut(s)	576, 2049
BstENI	CCTNNNNNAGG	1 cut(s)	4565
BstH2I	RGCGCY	1 cut(s)	3547
BstHHI	GCGC	3 cut(s)	200, 2839, 3546
BstNSI	RCATGY	4 cut(s)	1792, 1952, 4532, 4629
BstSFI	CTRYAG	8 cut(s)	115, 475, 1080, 2165, 2310, 3810, 4368, 4444
BstSLI	GKGCMC	7 cut(s)	8, 336, 474, 1735, 2722, 4560, 4687
BstV2I	GAAGAC	3 cut(s)	264, 3110, 3423
BstX2I	RGATCY	4 cut(s)	1348, 2618, 4851, 4976
BstYI	RGATCY	4 cut(s)	1348, 2618, 4851, 4976
BstZ17I	GTATAC	1 cut(s)	4578
Bsu15I	ATCGAT	1 cut(s)	5142
Bsu36I	CCTNAGG	3 cut(s)	966, 5266, 5388
BsuI	GTATCC	3 cut(s)	2350, 3228, 4549
BsuTUI	ATCGAT	1 cut(s)	5142
BtgI	CCRYGG	2 cut(s)	576, 2049
BtgZI	GCGATG	2 cut(s)	348, 4584
BtsI	GCAGTG	2 cut(s)	1831, 2164
BtsIMutI	CAGTG	7 cut(s)	426, 1725, 1831, 2164, 2977, 4587, 5384
BveI	ACCTGC	2 cut(s)	1216, 1245
CaiI	CAGNNNCTG	6 cut(s)	524, 965, 3014, 3547, 4436, 5041
CciI	TCATGA	1 cut(s)	5217
CfoI	GCGC	3 cut(s)	200, 2839, 3546
Cfr10I	RCCGGY	1 cut(s)	919
ClaI	ATCGAT	1 cut(s)	5142
CseI	GACGC	2 cut(s)	578, 1604
CsiI	ACCWGGT	1 cut(s)	2107
CspAI	ACCGGT	1 cut(s)	919
CspCI	CAANNNNNGTGG	4 cut(s)	1464, 1499, 5055, 5090
DraI	TTTAAA	1 cut(s)	2229
DraIII	CACNNNGTG	2 cut(s)	821, 2690
DriI	GACNNNNNGTC	1 cut(s)	4949
EaeI	YGGCCR	3 cut(s)	184, 3466, 5349
Eam1104I	CTCTTC	5 cut(s)	2625, 3306, 3524, 3690, 3870
Eam1105I	GACNNNNNGTC	1 cut(s)	4949
EarI	CTCTTC	5 cut(s)	2625, 3306, 3524, 3690, 3870
EciI	GGCGGA	1 cut(s)	3284
Ecl136II	GAGCTC	2 cut(s)	789, 1205
Eco130I	CCWWGG	6 cut(s)	576, 1489, 2454, 3996, 4060, 4722
Eco147I	AGGCCT	2 cut(s)	2184, 5264
Eco24I	GRGCYC	6 cut(s)	336, 791, 1207, 2722, 4281, 4560
Eco31I	GGTCTC	6 cut(s)	1988, 2775, 3797, 3872, 4433, 4662
Eco32I	GATATC	1 cut(s)	1097
Eco47I	GGWCC	8 cut(s)	455, 1564, 2091, 2967, 3538, 5032, 5282, 5382
Eco53kI	GAGCTC	2 cut(s)	789, 1205
Eco57I	CTGAAG	4 cut(s)	1592, 1931, 2063, 2634
Eco72I	CACGTG	1 cut(s)	469
Eco81I	CCTNAGG	3 cut(s)	966, 5266, 5388
Eco88I	CYCGRG	1 cut(s)	2302
EcoICRI	GAGCTC	2 cut(s)	789, 1205
EcoNI	CCTNNNNNAGG	1 cut(s)	4565
EcoO109I	RGGNCCY	3 cut(s)	333, 4556, 4557
EcoRV	GATATC	1 cut(s)	1097
EcoT14I	CCWWGG	6 cut(s)	576, 1489, 2454, 3996, 4060, 4722
EcoT22I	ATGCAT	2 cut(s)	1379, 4600
EcoT38I	GRGCYC	6 cut(s)	336, 791, 1207, 2722, 4281, 4560
ErhI	CCWWGG	6 cut(s)	576, 1489, 2454, 3996, 4060, 4722
Esp3I	CGTCTC	1 cut(s)	4312
FalI	AAGNNNNNCTT	8 cut(s)	1641, 1673, 2856, 2888, 3384, 3416, 4822, 4854
FaqI	GGGAC	5 cut(s)	104, 1727, 5249, 5368, 5384
FauI	CCCGC	1 cut(s)	4058
FauNDI	CATATG	3 cut(s)	892, 3250, 3747
FbaI	TGATCA	1 cut(s)	2794
FblI	GTMKAC	1 cut(s)	4577
FriOI	GRGCYC	6 cut(s)	336, 791, 1207, 2722, 4281, 4560
GlaI	GCGC	3 cut(s)	199, 2838, 3545
GsaI	CCCAGC	1 cut(s)	5320
GsuI	CTGGAG	3 cut(s)	2550, 2967, 4787
HaeII	RGCGCY	1 cut(s)	3547
HapII	CCGG	4 cut(s)	920, 3265, 4195, 4340
HgaI	GACGC	2 cut(s)	578, 1604
HhaI	GCGC	3 cut(s)	200, 2839, 3546
Hin6I	GCGC	3 cut(s)	198, 2837, 3544
HinP1I	GCGC	3 cut(s)	198, 2837, 3544
HincII	GTYRAC	2 cut(s)	37, 4057
HindII	GTYRAC	2 cut(s)	37, 4057
HindIII	AAGCTT	3 cut(s)	379, 2466, 2749
HpaII	CCGG	4 cut(s)	920, 3265, 4195, 4340
Hpy99I	CGWCG	1 cut(s)	1598
HpyCH4IV	ACGT	7 cut(s)	468, 1477, 2686, 4489, 4704, 4990, 5332
HpySE526I	ACGT	7 cut(s)	468, 1477, 2686, 4489, 4704, 4990, 5332
HspAI	GCGC	3 cut(s)	198, 2837, 3544
Ksp22I	TGATCA	1 cut(s)	2794
LguI	GCTCTTC	2 cut(s)	3306, 3690
MabI	ACCWGGT	1 cut(s)	2107
MaeII	ACGT	7 cut(s)	468, 1477, 2686, 4489, 4704, 4990, 5332
MfeI	CAATTG	2 cut(s)	1152, 3477
MflI	RGATCY	4 cut(s)	1348, 2618, 4851, 4976
MlsI	TGGCCA	2 cut(s)	3468, 5351
MluNI	TGGCCA	2 cut(s)	3468, 5351
MlyI	GAGTC	9 cut(s)	593, 1399, 1852, 2314, 2360, 3364, 3851, 4436, 4778
MmeI	TCCRAC	4 cut(s)	1134, 1254, 1373, 5324
Mox20I	TGGCCA	2 cut(s)	3468, 5351
Mph1103I	ATGCAT	2 cut(s)	1379, 4600
MroXI	GAANNNNTTC	3 cut(s)	1456, 2634, 5333
MscI	TGGCCA	2 cut(s)	3468, 5351
MslI	CAYNNNNRTG	8 cut(s)	687, 756, 864, 1608, 1824, 2529, 3798, 4215
Msp20I	TGGCCA	2 cut(s)	3468, 5351
MspA1I	CMGCKG	4 cut(s)	114, 3071, 4037, 4668
MspCI	CTTAAG	1 cut(s)	1316
MspI	CCGG	4 cut(s)	920, 3265, 4195, 4340
MunI	CAATTG	2 cut(s)	1152, 3477
Mva1269I	GAATGC	3 cut(s)	751, 4420, 5126
NciI	CCSGG	2 cut(s)	3266, 4196
NcoI	CCATGG	1 cut(s)	576
NdeI	CATATG	3 cut(s)	892, 3250, 3747
NheI	GCTAGC	1 cut(s)	2153
NmuCI	GTSAC	4 cut(s)	4108, 4485, 4582, 4750
NsiI	ATGCAT	2 cut(s)	1379, 4600
NspI	RCATGY	4 cut(s)	1792, 1952, 4532, 4629
NspV	TTCGAA	1 cut(s)	2731
PagI	TCATGA	1 cut(s)	5217
PasI	CCCWGGG	1 cut(s)	4687
PceI	AGGCCT	2 cut(s)	2184, 5264
PciI	ACATGT	2 cut(s)	1788, 1948
PciSI	GCTCTTC	2 cut(s)	3306, 3690
PcsI	WCGNNNNNNNCGW	1 cut(s)	1054
PctI	GAATGC	3 cut(s)	751, 4420, 5126
PdmI	GAANNNNTTC	3 cut(s)	1456, 2634, 5333
PflMI	CCANNNNNTGG	2 cut(s)	248, 2129
PfoI	TCCNGGA	1 cut(s)	4194
PinAI	ACCGGT	1 cut(s)	919
PleI	GAGTC	9 cut(s)	592, 1398, 1852, 2313, 2360, 3364, 3850, 4435, 4777
PmaCI	CACGTG	1 cut(s)	469
PmlI	CACGTG	1 cut(s)	469
PpsI	GAGTC	9 cut(s)	592, 1398, 1852, 2313, 2360, 3364, 3850, 4435, 4777
Ppu21I	YACGTR	1 cut(s)	469
PscI	ACATGT	2 cut(s)	1788, 1948
PshBI	ATTAAT	1 cut(s)	864
PsiI	TTATAA	1 cut(s)	4995
Psp124BI	GAGCTC	2 cut(s)	791, 1207
Psp1406I	AACGTT	2 cut(s)	4704, 5332
PspCI	CACGTG	1 cut(s)	469
PspFI	CCCAGC	1 cut(s)	5316
PspOMI	GGGCCC	3 cut(s)	332, 2718, 4556
PstI	CTGCAG	3 cut(s)	2169, 3814, 4372
PstNI	CAGNNNCTG	6 cut(s)	524, 965, 3014, 3547, 4436, 5041
PsuI	RGATCY	4 cut(s)	1348, 2618, 4851, 4976
PvuII	CAGCTG	2 cut(s)	114, 3071
RseI	CAYNNNNRTG	8 cut(s)	687, 756, 864, 1608, 1824, 2529, 3798, 4215
SacI	GAGCTC	2 cut(s)	791, 1207
SapI	GCTCTTC	2 cut(s)	3306, 3690
ScaI	AGTACT	1 cut(s)	4825
SchI	GAGTC	9 cut(s)	593, 1399, 1852, 2314, 2360, 3364, 3851, 4436, 4778
SexAI	ACCWGGT	1 cut(s)	2107
SfcI	CTRYAG	8 cut(s)	115, 475, 1080, 2165, 2310, 3810, 4368, 4444
SfuI	TTCGAA	1 cut(s)	2731
SinI	GGWCC	8 cut(s)	455, 1564, 2091, 2967, 3538, 5032, 5282, 5382
SmiMI	CAYNNNNRTG	8 cut(s)	687, 756, 864, 1608, 1824, 2529, 3798, 4215
SmlI	CTYRAG	5 cut(s)	1316, 1647, 2953, 3488, 3614
SmoI	CTYRAG	5 cut(s)	1316, 1647, 2953, 3488, 3614
SpeI	ACTAGT	3 cut(s)	3950, 4112, 4283
SseBI	AGGCCT	2 cut(s)	2184, 5264
SspI	AATATT	2 cut(s)	1690, 4805
SstI	GAGCTC	2 cut(s)	791, 1207
StuI	AGGCCT	2 cut(s)	2184, 5264
StyI	CCWWGG	6 cut(s)	576, 1489, 2454, 3996, 4060, 4722
TaiI	ACGT	7 cut(s)	471, 1480, 2689, 4492, 4707, 4993, 5335
TatI	WGTACW	5 cut(s)	206, 3677, 3856, 3953, 4823
TauI	GCSGC	3 cut(s)	3926, 4668, 5095
TscAI	CASTG	7 cut(s)	433, 1732, 1831, 2164, 2977, 4587, 5384
TseFI	GTSAC	4 cut(s)	4108, 4485, 4582, 4750
Tsp45I	GTSAC	4 cut(s)	4108, 4485, 4582, 4750
TspGWI	ACGGA	4 cut(s)	2038, 3592, 4775, 4960
TspRI	CASTG	7 cut(s)	433, 1732, 1831, 2164, 2977, 4587, 5384
Van91I	CCANNNNNTGG	2 cut(s)	248, 2129
Vha464I	CTTAAG	1 cut(s)	1316
VpaK11BI	GGWCC	8 cut(s)	455, 1564, 2091, 2967, 3538, 5032, 5282, 5382
VspI	ATTAAT	1 cut(s)	864
XagI	CCTNNNNNAGG	1 cut(s)	4565
XapI	RAATTY	5 cut(s)	1805, 3038, 3209, 3578, 3664
XbaI	TCTAGA	1 cut(s)	3962
XceI	RCATGY	4 cut(s)	1792, 1952, 4532, 4629
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1180
XmiI	GTMKAC	1 cut(s)	4577
XmnI	GAANNNNTTC	3 cut(s)	1456, 2634, 5333
ZrmI	AGTACT	1 cut(s)	4825
Zsp2I	ATGCAT	2 cut(s)	1379, 4600

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.