Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002065

Physical Location & Seq

Genomic Coordinates Forward (+)

7834117 .. 7843009

Gene Structure 8893 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G007990.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5457 bp

ATGGGCACCCCTGACAACAAATTGTCAGAGATAGTTGACATTTTCAAGTCTTGGATGCCCAGGAGAACCGAGCCAACCAATGTGTCCAGGGACTTTTGGATGCCCGATCACAGCTGTAGGGTATGCTACGACTGTGATTCCCAGTTCACAGTATTTAATCGAAGGCATCATTGCCGCCTTTGTGGCCGAGTTTTCTGCGCTAGGTGTACTGCAAACACTATTCCTGCCCCATCTGATGAGCCAAGAGTTGGTAGGGAAGACGGGGAGAGGATTAGGGTCTGTAATTTTTGCTTCAAGCAATGGGAGCAAGGGATAGCAGCAGTTGATAATGGGCCCCCTGAACCTAGCCCTGGCCTCAGTCCATCACCATCTACAACAAGCTTGGTCAGTACCAACACAAGCTGCACCTACCACAGTAGCAGCAGCACTGTTGGTTCTACCCCTTATTCGACTGGACCTTACCAGCACGTGCCCTATAGTTCTGGTCTTAGCCCCAGACAATCTTGCCAAGATGATTCAGTTACTGGCCTGCAAGACAATGTAACATCTCAGAGGAGCATTAGTTCTGACACTGCCATGGCAGAGTCGTCTCCCAACCAGTTTGGCTTTTGCATGGACAGGAGTGATGATGAGGATGATGACTATGGTTATCATTCAGATTCAGAATCAAGACATTTCACCCATGCCAATGACTATTATGGTGCAATCAATAACGAGGAGTTTGATAGCGTGTATGAACCACAGAAGGTGCATTCTGATGGAGAAACCACAGATGCAAAAAGTTTGAGCTCCTTTTCACCTGAGAAGTTTGATACACAGAGTGTAGTGGGAACGAAAGTTGGGGAAGAATCAGATTATCGTATTAATTGTGATGAATGTGAAACTTCTCCATATGAGATGGAGACTACAAATGCAGAACCGGTGGATTTTGAGAATAATGGGCTACTTTGGCTCCCACCAGAACCTGAGGATGAAGAAGATGACAGAGAAGCTGCCTTATTTGAGGATGATGATGACGATGAGGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGCTACAGGGGAGTGGGGATATCTTCATTCTTCAAACAGTTTTGGTGGTGGGGAGTGTCGTACTAGGGAGAAGTCAATTGAGGAGCACAGGAAGGCCATGAAGAATGTGGTGGAAGGTCATTTTAGAGCTCTGGTATCTCAGCTCTTGCAGGTTGAAAACCTGCCTTCGATTGATGAAAATTACAAAGAGACTTGGTTGGACATAATTACGTCTTTGTCATGGGAAGCTGCCACACTTCTTAAGCCAGATACGAGCAAAGGGGGAGGAATGGATCCTGGGGGATATGTGAAGGTTAAATGCATAGCTTGTGGGAGTCGGAGCGAGAGCATGGTGGTTAAAGGAGTTGTTTGCAAGAAAAATGTGGCTCATCGGCGAATGACTTCAAAGATAGAAAAACCACGTTTCTTGATCCTTGGAGGAGCTTTGGAATATCAGCGTGTATCAAACCACTTGTCCAGTTTCGATACTTTGTTGCAGCAGGAGATGGACCATCTGAAGATGGCAGTTGCTAAGATCGATGTACATCACCCTAATGTTTTATTGGTGGAGAAGTCTGTATCTCGGTATGCTCAAGATTACCTTCTTGCGAAAGACATATCACTCGTTCTGAATATTAAGAGGCCACTTTTGGAGCGAATATCTCGCTGCACTGGGGCACAAATTGTCCCTTCGATAGATCATCTTACATCACCAAAACTAGGGTATTGCGACATGTTCCATGTGGAGAAATTTTTTGAAGTGCATGGCAGTGCTGGACAAGGTGGGAAGAAATTAACAAAGACTCTGATGTTCTTTGAAGGTTGTCCAAAGCCATTGGGTGTTACTATATTGCTGAAGGGTGCCAATGGGGATGAGTTAAAAAAGGTGAAACATGTTGTGCAGTATGGAGTTTTTGCAGCTTATCACTTGGCTTTGGAGACCTCATTTTTAGCTGATGAAGGAGCTTCCCTTCCAGAACTTCCTCTGAAGTCTGTGGTAACTGTATTGCCTGATAAACCGTCGAGTATTGACAGGTCCATTTCCACCATACCTGGTTTTAGTGTACCAGCAGCTGGCAAGCCTCAGAGTTCCAATGCTAGCAGTGAACTGCAGAACTCAAATAAAGGCCTCATTTCAGATAGTGGCTCATTTGCCCAAGTTGCCTCCATTTTAAAGAACGAAGGCACCAATTTGGTCTGCTTACCTAAAGCTCCGTGTTCTCAACCTTCCCCCATAAAACATACCCCGAGTCCTATAGAATATACATCTTCTTTCACTTCCTTGTCTCCCCCAGGACAGGATACGACAGACTCCTACCATAAAGAGCTTTCTTCAATTTGTGGCTCTGAGGACAACAAGGATGTAAGTTCTAAAGAATCTTGTCCAGCCAAAACAGCCAAGGCAGGCGAAGCTTTAGTTCACGATAGTTTGATCTCTAATTCTTTTAGCACCTCAGAGGCATTTGGACATGGTGGTGGAATTGGCAATGCTGATGGCGTTGCTTTAGCTGCAAATCTCAGTGAGACTCCAGAGCTTCCATCCATAAAATATCATACTGATAATCAGAATGAGGAGGTGGGATCTTCAAAAGAAGAGTTTCCCCCCTCACCTTCAGACCACCAGAGCATTTTGGTTTCCCTATCAACACGTTGTGTGTGGAAAGGAACTGTATGTGAACGAGCCCATCTTTTTCGAATCAAATACTATGGAAGCTTTGATAAGCCTCTGGGGAGGTTTTTACGAGACCATCTTTTTGATCAGGGTTACCTCTGTCGTTCATGTGGGATGCCCTCAGAAGCGCATATTCATTGTTATACTCATAGACAAGGAAGCCTTACAATATCTGTTAAGAAACTCCCAGAGATTTTCCTGCCAGGGGAAAAGGAAGGAAAAATATGGATGTGGCATAGGTGCTTGAGATGTCCTCGGACCAGTGGCTTCCCTCCAGCCACTCGTAGAGTTGTAATGTCAGATGCTGCTTGGGGTTTATCTTTTGGGAAATTTCTGGAATTGAGCTTTTCAAATCATGCAGCTGCAAACAGAGTTGCAAGCTGTGGTCATTCCCTGCACAGAGATTGTCTTCGGTTTTATGGGTTTGGGAGAATGGTTGCTTGCTTCCGCTATGCCTCAATTCATATTCACTCTGTTTGTCTGCCCCCACCAAAACTTGAATTTTACTATGATAATCAGGAATGGATACAAAAAGAAGCATACGAGGTGGGCAACCGGGTGGAGCTTCTATTTACTGAATTACATAGTGCTCTTCATCAGATTTTACAGAAGATAAAAGTTGTGGAGACACAGGATGGTGGTAAGAAAGCACCTGAATCAACTCATCAAATTGTAGAACTTGAAGGGATGCTTCAAAAGGAAAGGGAAGACTTTGAGAAATCATTACAAAAAGTGATAAAGGAGGAGGTGAAATCTGGCCAGCCTGCAATTGATATTCTTGAGATCAATAAATTGCGGAGGCAGTTACTCTTCCATTCTTATGTTTGGGACCAGCGCCTGATTCGTGCAGCCGGTCTAAGCAATAATAAATTTCAGGAAGGTTTAACCAGTTCCGTTACCAAACTCAAGGAGAAACCTATTGGCATGGAGAAGGTTGTCAAAACACCAGGAAGAAATTTCAGTAGTTGTACAGCTCTTCCCGAAATCAAGCCTGGTATAAATCTTAATCAAGGAGGAGATGCTGGCTACTTTAGCCAGATGGGAGGGGTTCAGAACAGAACTGAAATGGGTCTGGACACAGATCATGGGAATGAGACCTCTGCAAATGTCCTTGATAAATCTGATCCTTTGGAATCTGAAAAAATTGTACAAATGGGTCTCTCGGAAGAGAATGAATGTTCGGCTGTGGAAAGTTTATCTGATACCCTTGATGCGGCTTGGACTGGTACAACCCACCCAACTAGTACAACATCTAGAGAGAATGGCTATTCGCTTCCTCATCCAACCTTGGTAAAATCATCAGCTGTGGTAAAAAATGTTGCATCCGCTGTGGAAAATGGTACTGTTGACCATGGTGGGGTACAGGCAACTCGTTCTCTTATTTCTACATTTCCTGCTGTCACTAGTTCATTCAGTAAAAGTGCTTCCTTTAACACTCAAAAGCTATGTATTGGTGGTGAGCACAGTCCTGTCTATGTTGCACGGTTCCGGGAACTGGAACGGCATACTGGTGCCAGGTTGCTACTGCCGATTGGTGTTAATGATACAGTTATCCCTGTTTTTGATGATGAGCCCACTAGTGTTATTGCATATACCCTCGTCTCTCCTAATTATCATTTGCAGATTTCTGAGCCTGATAGACCAATTGATTCTGCAGTCTCATTACCGCTTTTTGATTCAGCAAATCTTCTCTCACTGAATGCTTTTGATGAGTCAGTTTCTGAGACCTACAGAGGTCTTAGTTCTTCTGATGAGAGCATCTTATCCATGTCACATTCACGGAGCTCTGAATCTCTTGTGTCAAAGGATATACATGCTAGAGTATCCTTTACAGATGAGGGGCCCCTCGGTAAGGTGAAGTATACAGTGACTTGTTACTACGCATCGCAGTTTGAGGCATTAAGGAAGGCATGTTGCCCTTCCGAATTAGATTTTGTGCGGTCTCTTAGCCGCTGTAAGAAGTGGGGTGCCCAGGGTGGAAAGAGCAACGTTTTCTTTGCTAAAACCTTGGATGATCGGTTTATCATCAAACAAGTCACAAAGACGGAGCTGGAGTCATTTATCAAGTTTGCACCAGCTTATTTCAAATATTTGTCTCATTCAATCAGTACTAGAAGCCCAACTTGCCTAGCAAAGATCCTGGGTATCTATCAGGTTTCATCAAAACATGGTAAAGCAGGGAAAGAGACAAAGATGGATGTCTTAGTAATGGAGAATCTATTATTTAGACGGAATGTCTCACGGCTCTATGACCTGAAAGGATCTTCTCGATCACGTTATAATCCAGACACAAGTGGAAGCAATAAAGTGCTGCTGGACCAGAATCTGATTGAGGCAATGCCTACTTCTCCAATTTTTTTGGGGAACAGGGCAAAGCGGCTATTGGAGAGAGCTGTCTGGAATGATACTGCATTCCTTGCTTCAATCGATGTGATGGACTACTCATTACTGGTTGGAGTGGATGAGGAAAAGCACGAGCTAGTTTTGGGGATCATTGATTTCATGAGGCAATATACATGGGACAAGCACCTTGAAACTTGGGTGAAGGCCTCAGGCATCCTCGGAGGACCGAAGAATACATCTCCAACCGTGATTTCACCCCAGCAGTATAAGAAACGTTTCAGGAAAGCTATGGCCACTTACTTTCTGATGCTTCCCGATCAGTGGTCGCCTGAGGCGATTGTCCCAAGTGGGTCGCAATCTGACCATTTTGAAGAAACTTCCCAAGCTCAATCTGAAGCCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1818

Amino Acids

200.61

Weight (kDa)

5.85

Isoelectric Point (pI)

48.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	3.8e-17	FYVE zinc finger
Cpn60_TCP1	PF00118	415 - 664	4.1e-30	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1561 - 1727	1.2e-34	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1726 - 1782	6.7e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240
fragaria_vesca	FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530
malus_domestica	MD02G1103900.v1.1 MD15G1224300.v1.1
prunus_persica	Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1
pyrus_communis	pycom02g08170 pycom15g19930 pycom15g19940
rosa_chinensis	RchiOBHm_Chr2g0096101
rosa_laevigata	RLG00000016625
rosa_multiflora	Rmu_sc0005996.1_g000006
rosa_roxburghii	Rroxscaffold_2G00145750
rosa_rugosa	Rorug02G0057000 Rorug02G0057100 Rorug02G0057200 Rorug02G0057200
rosa_samantha	Rh2AG104000 Rh2BG106100 Rh2CG108000 Rh2DG106700
rosa_wichuraiana	Rw2G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4989
Acc36I	ACCTGC	2 cut(s)	1198, 1227
AccB1I	GGYRCC	5 cut(s)	5, 1898, 2222, 4220, 4676
AccB7I	CCANNNNNTGG	2 cut(s)	248, 2111
AccI	GTMKAC	1 cut(s)	4571
AciI	CCGC	9 cut(s)	175, 3157, 3505, 3923, 4035, 4376, 4648, 4660, 5086
AclI	AACGTT	2 cut(s)	4698, 5326
AclWI	GGATC	8 cut(s)	1325, 1338, 1462, 2626, 3827, 4840, 4978, 5207
AcoI	YGGCCR	3 cut(s)	184, 3466, 5343
AcsI	RAATTY	5 cut(s)	1787, 3038, 3209, 3578, 3664
AcuI	CTGAAG	4 cut(s)	1574, 1913, 2045, 2634
AcvI	CACGTG	1 cut(s)	469
AdeI	CACNNNGTG	2 cut(s)	821, 2690
AflII	CTTAAG	1 cut(s)	1298
AflIII	ACRYGT	3 cut(s)	1770, 1930, 2684
AgeI	ACCGGT	1 cut(s)	919
AhdI	GACNNNNNGTC	1 cut(s)	4943
AhlI	ACTAGT	3 cut(s)	3950, 4112, 4286
AjuI	GAANNNNNNNTTGG	2 cut(s)	5287, 5319
AloI	GAACNNNNNNTCC	2 cut(s)	547, 579
Alw21I	GWGCWC	6 cut(s)	791, 1146, 1189, 3301, 4173, 4496
AlwI	GGATC	8 cut(s)	1325, 1338, 1462, 2626, 3827, 4840, 4978, 5207
AlwNI	CAGNNNCTG	7 cut(s)	524, 965, 2111, 3014, 3547, 4430, 5035
Ama87I	CYCGRG	1 cut(s)	2284
ApaI	GGGCCC	2 cut(s)	336, 4554
ApoI	RAATTY	5 cut(s)	1787, 3038, 3209, 3578, 3664
ArsI	GACNNNNNNTTYG	4 cut(s)	1496, 1528, 2774, 2806
AseI	ATTAAT	1 cut(s)	864
AsiGI	ACCGGT	1 cut(s)	919
Asp700I	GAANNNNTTC	3 cut(s)	1438, 2634, 5327
AspLEI	GCGC	3 cut(s)	200, 2839, 3546
AsuC2I	CCSGG	2 cut(s)	3266, 4199
AsuII	TTCGAA	1 cut(s)	2731
AsuNHI	GCTAGC	1 cut(s)	2135
AvaI	CYCGRG	1 cut(s)	2284
AvaII	GGWCC	7 cut(s)	455, 1546, 2073, 2967, 3538, 5026, 5276
AxyI	CCTNAGG	3 cut(s)	966, 5260, 5382
BaeGI	GKGCMC	6 cut(s)	8, 336, 474, 1717, 4554, 4681
BalI	TGGCCA	2 cut(s)	3468, 5345
BamHI	GGATCC	1 cut(s)	1330
BanI	GGYRCC	5 cut(s)	5, 1898, 2222, 4220, 4676
BanII	GRGCYC	7 cut(s)	336, 791, 1189, 2722, 4284, 4496, 4554
BarI	GAAGNNNNNNTAC	2 cut(s)	4117, 4149
BauI	CACGAG	1 cut(s)	5183
BbrPI	CACGTG	1 cut(s)	469
BbsI	GAAGAC	3 cut(s)	264, 3110, 3423
Bbv12I	GWGCWC	6 cut(s)	791, 1146, 1189, 3301, 4173, 4496
BceAI	ACGGC	2 cut(s)	4226, 4967
BcgI	CGANNNNNNTGC	2 cut(s)	177, 211
BciVI	GTATCC	3 cut(s)	2332, 3228, 4543
BclI	TGATCA	1 cut(s)	2794
BcnI	CCSGG	2 cut(s)	3266, 4199
BcuI	ACTAGT	3 cut(s)	3950, 4112, 4286
BfmI	CTRYAG	7 cut(s)	115, 475, 1062, 2147, 2292, 4362, 4438
BfoI	RGCGCY	1 cut(s)	3547
BfrI	CTTAAG	1 cut(s)	1298
BfuAI	ACCTGC	2 cut(s)	1198, 1227
BfuI	GTATCC	3 cut(s)	2332, 3228, 4543
BglI	GCCNNNNNGGC	1 cut(s)	183
BmcAI	AGTACT	1 cut(s)	4819
Bme18I	GGWCC	7 cut(s)	455, 1546, 2073, 2967, 3538, 5026, 5276
BmeRI	GACNNNNNGTC	1 cut(s)	4943
BmeT110I	CYCGRG	1 cut(s)	2284
BmrI	ACTGGG	2 cut(s)	136, 1719
BmtI	GCTAGC	1 cut(s)	2139
BmuI	ACTGGG	2 cut(s)	136, 1719
BpiI	GAAGAC	3 cut(s)	264, 3110, 3423
BplI	GAGNNNNNCTC	2 cut(s)	2947, 2979
BpmI	CTGGAG	3 cut(s)	2550, 2967, 4781
Bpu14I	TTCGAA	1 cut(s)	2731
BpuEI	CTTGAG	4 cut(s)	1614, 2974, 3509, 3599
BpuMI	CCSGG	2 cut(s)	3266, 4199
Bsa29I	ATCGAT	2 cut(s)	1575, 5136
BsaAI	YACGTR	1 cut(s)	469
BsaBI	GATNNNNATC	1 cut(s)	1581
BsaI	GGTCTC	6 cut(s)	1970, 2775, 3797, 3872, 4427, 4656
BsaWI	WCCGGW	1 cut(s)	919
BsaXI	ACNNNNNCTCC	6 cut(s)	547, 577, 936, 966, 4748, 4778
Bse118I	RCCGGY	2 cut(s)	919, 3560
Bse21I	CCTNAGG	3 cut(s)	966, 5260, 5382
Bse3DI	GCAATG	4 cut(s)	169, 305, 2530, 5052
Bse8I	GATNNNNATC	1 cut(s)	1581
BseCI	ATCGAT	2 cut(s)	1575, 5136
BseJI	GATNNNNATC	1 cut(s)	1581
BseMI	GCAATG	4 cut(s)	169, 305, 2530, 5052
BseRI	GAGGAG	7 cut(s)	568, 731, 1154, 1491, 2624, 3467, 3738
BseSI	GKGCMC	6 cut(s)	8, 336, 474, 1717, 4554, 4681
BseYI	CCCAGC	1 cut(s)	5310
BsgI	GTGCAG	5 cut(s)	388, 1690, 1958, 3089, 3576
BshNI	GGYRCC	5 cut(s)	5, 1898, 2222, 4220, 4676
BshTI	ACCGGT	1 cut(s)	919
BshVI	ATCGAT	2 cut(s)	1575, 5136
BsiHKAI	GWGCWC	6 cut(s)	791, 1146, 1189, 3301, 4173, 4496
BsiHKCI	CYCGRG	1 cut(s)	2284
BsiSI	CCGG	4 cut(s)	920, 3265, 3561, 4198
BslFI	GGGAC	5 cut(s)	104, 1709, 3551, 5243, 5378
BsmBI	CGTCTC	2 cut(s)	594, 4315
BsmFI	GGGAC	5 cut(s)	104, 1709, 3551, 5243, 5378
BsmI	GAATGC	3 cut(s)	751, 4414, 5120
Bso31I	GGTCTC	6 cut(s)	1970, 2775, 3797, 3872, 4427, 4656
BsoBI	CYCGRG	1 cut(s)	2284
Bsp119I	TTCGAA	1 cut(s)	2731
Bsp120I	GGGCCC	2 cut(s)	332, 4550
Bsp1407I	TGTACA	3 cut(s)	1579, 3677, 3856
Bsp19I	CCATGG	2 cut(s)	576, 4060
BspACI	CCGC	9 cut(s)	175, 3157, 3505, 3923, 4035, 4376, 4648, 4660, 5086
BspDI	ATCGAT	2 cut(s)	1575, 5136
BspHI	TCATGA	1 cut(s)	5211
BspMAI	CTGCAG	2 cut(s)	2151, 4366
BspMI	ACCTGC	2 cut(s)	1198, 1227
BspOI	GCTAGC	1 cut(s)	2139
BspPI	GGATC	8 cut(s)	1325, 1338, 1462, 2626, 3827, 4840, 4978, 5207
BspQI	GCTCTTC	2 cut(s)	3306, 3690
BspT104I	TTCGAA	1 cut(s)	2731
BspT107I	GGYRCC	5 cut(s)	5, 1898, 2222, 4220, 4676
BspTI	CTTAAG	1 cut(s)	1298
BspTNI	GGTCTC	6 cut(s)	1970, 2775, 3797, 3872, 4427, 4656
BsrDI	GCAATG	4 cut(s)	169, 305, 2530, 5052
BsrFI	RCCGGY	2 cut(s)	919, 3560
BsrGI	TGTACA	3 cut(s)	1579, 3677, 3856
BssAI	RCCGGY	2 cut(s)	919, 3560
BssNAI	GTATAC	1 cut(s)	4572
BssSI	CACGAG	1 cut(s)	5183
BssT1I	CCWWGG	6 cut(s)	576, 1471, 2436, 3996, 4060, 4716
Bst1107I	GTATAC	1 cut(s)	4572
Bst2BI	CACGAG	1 cut(s)	5183
Bst6I	CTCTTC	5 cut(s)	2625, 3306, 3524, 3690, 3870
BstAFI	CTTAAG	1 cut(s)	1298
BstAPI	GCANNNNNTGC	1 cut(s)	5126
BstAUI	TGTACA	3 cut(s)	1579, 3677, 3856
BstBAI	YACGTR	1 cut(s)	469
BstBI	TTCGAA	1 cut(s)	2731
BstDSI	CCRYGG	2 cut(s)	576, 4060
BstEII	GGTNACC	1 cut(s)	2801
BstENI	CCTNNNNNAGG	1 cut(s)	4559
BstH2I	RGCGCY	1 cut(s)	3547
BstHHI	GCGC	3 cut(s)	200, 2839, 3546
BstNSI	RCATGY	4 cut(s)	1774, 1934, 4526, 4623
BstPI	GGTNACC	1 cut(s)	2801
BstSFI	CTRYAG	7 cut(s)	115, 475, 1062, 2147, 2292, 4362, 4438
BstSLI	GKGCMC	6 cut(s)	8, 336, 474, 1717, 4554, 4681
BstV2I	GAAGAC	3 cut(s)	264, 3110, 3423
BstX2I	RGATCY	4 cut(s)	1330, 2618, 4845, 4970
BstYI	RGATCY	4 cut(s)	1330, 2618, 4845, 4970
BstZ17I	GTATAC	1 cut(s)	4572
Bsu15I	ATCGAT	2 cut(s)	1575, 5136
Bsu36I	CCTNAGG	3 cut(s)	966, 5260, 5382
BsuI	GTATCC	3 cut(s)	2332, 3228, 4543
BsuTUI	ATCGAT	2 cut(s)	1575, 5136
BtgI	CCRYGG	2 cut(s)	576, 4060
BtgZI	GCGATG	1 cut(s)	4578
BtsI	GCAGTG	3 cut(s)	570, 1813, 2146
BveI	ACCTGC	2 cut(s)	1198, 1227
CaiI	CAGNNNCTG	7 cut(s)	524, 965, 2111, 3014, 3547, 4430, 5035
CciI	TCATGA	1 cut(s)	5211
CfoI	GCGC	3 cut(s)	200, 2839, 3546
Cfr10I	RCCGGY	2 cut(s)	919, 3560
ClaI	ATCGAT	2 cut(s)	1575, 5136
CsiI	ACCWGGT	1 cut(s)	2089
CspAI	ACCGGT	1 cut(s)	919
CspCI	CAANNNNNGTGG	2 cut(s)	1446, 1481
DraI	TTTAAA	1 cut(s)	2211
DraIII	CACNNNGTG	2 cut(s)	821, 2690
DriI	GACNNNNNGTC	1 cut(s)	4943
EaeI	YGGCCR	3 cut(s)	184, 3466, 5343
Eam1104I	CTCTTC	5 cut(s)	2625, 3306, 3524, 3690, 3870
Eam1105I	GACNNNNNGTC	1 cut(s)	4943
EarI	CTCTTC	5 cut(s)	2625, 3306, 3524, 3690, 3870
Ecl136II	GAGCTC	3 cut(s)	789, 1187, 4494
Eco130I	CCWWGG	6 cut(s)	576, 1471, 2436, 3996, 4060, 4716
Eco147I	AGGCCT	2 cut(s)	2166, 5258
Eco24I	GRGCYC	7 cut(s)	336, 791, 1189, 2722, 4284, 4496, 4554
Eco31I	GGTCTC	6 cut(s)	1970, 2775, 3797, 3872, 4427, 4656
Eco32I	GATATC	1 cut(s)	1079
Eco47I	GGWCC	7 cut(s)	455, 1546, 2073, 2967, 3538, 5026, 5276
Eco53kI	GAGCTC	3 cut(s)	789, 1187, 4494
Eco57I	CTGAAG	4 cut(s)	1574, 1913, 2045, 2634
Eco72I	CACGTG	1 cut(s)	469
Eco81I	CCTNAGG	3 cut(s)	966, 5260, 5382
Eco88I	CYCGRG	1 cut(s)	2284
Eco91I	GGTNACC	1 cut(s)	2801
EcoICRI	GAGCTC	3 cut(s)	789, 1187, 4494
EcoNI	CCTNNNNNAGG	1 cut(s)	4559
EcoO109I	RGGNCCY	3 cut(s)	333, 4550, 4551
EcoO65I	GGTNACC	1 cut(s)	2801
EcoRV	GATATC	1 cut(s)	1079
EcoT14I	CCWWGG	6 cut(s)	576, 1471, 2436, 3996, 4060, 4716
EcoT22I	ATGCAT	1 cut(s)	1361
EcoT38I	GRGCYC	7 cut(s)	336, 791, 1189, 2722, 4284, 4496, 4554
ErhI	CCWWGG	6 cut(s)	576, 1471, 2436, 3996, 4060, 4716
Esp3I	CGTCTC	2 cut(s)	594, 4315
FalI	AAGNNNNNCTT	8 cut(s)	1623, 1655, 2856, 2888, 3384, 3416, 4816, 4848
FaqI	GGGAC	5 cut(s)	104, 1709, 3551, 5243, 5378
FauNDI	CATATG	1 cut(s)	892
FbaI	TGATCA	1 cut(s)	2794
FblI	GTMKAC	1 cut(s)	4571
FriOI	GRGCYC	7 cut(s)	336, 791, 1189, 2722, 4284, 4496, 4554
GlaI	GCGC	3 cut(s)	199, 2838, 3545
GsaI	CCCAGC	1 cut(s)	5314
GsuI	CTGGAG	3 cut(s)	2550, 2967, 4781
HaeII	RGCGCY	1 cut(s)	3547
HapII	CCGG	4 cut(s)	920, 3265, 3561, 4198
HhaI	GCGC	3 cut(s)	200, 2839, 3546
Hin6I	GCGC	3 cut(s)	198, 2837, 3544
HinP1I	GCGC	3 cut(s)	198, 2837, 3544
HincII	GTYRAC	2 cut(s)	37, 4057
HindII	GTYRAC	2 cut(s)	37, 4057
HindIII	AAGCTT	3 cut(s)	379, 2448, 2749
HpaII	CCGG	4 cut(s)	920, 3265, 3561, 4198
Hpy166II	GTNNAC	9 cut(s)	37, 147, 207, 2102, 2144, 2458, 2714, 4057, 4572
Hpy8I	GTNNAC	9 cut(s)	37, 147, 207, 2102, 2144, 2458, 2714, 4057, 4572
Hpy99I	CGWCG	1 cut(s)	2062
HpyCH4IV	ACGT	7 cut(s)	468, 1268, 1459, 2686, 4698, 4984, 5326
HpySE526I	ACGT	7 cut(s)	468, 1268, 1459, 2686, 4698, 4984, 5326
HspAI	GCGC	3 cut(s)	198, 2837, 3544
Ksp22I	TGATCA	1 cut(s)	2794
LguI	GCTCTTC	2 cut(s)	3306, 3690
MabI	ACCWGGT	1 cut(s)	2089
MaeII	ACGT	7 cut(s)	468, 1268, 1459, 2686, 4698, 4984, 5326
MfeI	CAATTG	3 cut(s)	1134, 3477, 4353
MflI	RGATCY	4 cut(s)	1330, 2618, 4845, 4970
MlsI	TGGCCA	2 cut(s)	3468, 5345
MluNI	TGGCCA	2 cut(s)	3468, 5345
MlyI	GAGTC	8 cut(s)	593, 1381, 1834, 2296, 2342, 2557, 4430, 4772
MmeI	TCCRAC	5 cut(s)	1236, 1355, 4016, 5143, 5318
Mox20I	TGGCCA	2 cut(s)	3468, 5345
Mph1103I	ATGCAT	1 cut(s)	1361
MroXI	GAANNNNTTC	3 cut(s)	1438, 2634, 5327
MscI	TGGCCA	2 cut(s)	3468, 5345
MslI	CAYNNNNRTG	9 cut(s)	575, 687, 756, 1590, 1806, 2511, 3528, 3798, 4218
Msp20I	TGGCCA	2 cut(s)	3468, 5345
MspA1I	CMGCKG	6 cut(s)	114, 2111, 3071, 4013, 4037, 4662
MspCI	CTTAAG	1 cut(s)	1298
MspI	CCGG	4 cut(s)	920, 3265, 3561, 4198
MunI	CAATTG	3 cut(s)	1134, 3477, 4353
Mva1269I	GAATGC	3 cut(s)	751, 4414, 5120
NciI	CCSGG	2 cut(s)	3266, 4199
NcoI	CCATGG	2 cut(s)	576, 4060
NdeI	CATATG	1 cut(s)	892
NheI	GCTAGC	1 cut(s)	2135
NmeAIII	GCCGAG	1 cut(s)	212
NmuCI	GTSAC	4 cut(s)	4108, 4479, 4576, 4744
NsiI	ATGCAT	1 cut(s)	1361
NspI	RCATGY	4 cut(s)	1774, 1934, 4526, 4623
NspV	TTCGAA	1 cut(s)	2731
PagI	TCATGA	1 cut(s)	5211
PasI	CCCWGGG	1 cut(s)	4681
PceI	AGGCCT	2 cut(s)	2166, 5258
PciI	ACATGT	2 cut(s)	1770, 1930
PciSI	GCTCTTC	2 cut(s)	3306, 3690
PcsI	WCGNNNNNNNCGW	1 cut(s)	5384
PctI	GAATGC	3 cut(s)	751, 4414, 5120
PdmI	GAANNNNTTC	3 cut(s)	1438, 2634, 5327
PflMI	CCANNNNNTGG	2 cut(s)	248, 2111
PfoI	TCCNGGA	1 cut(s)	4197
PinAI	ACCGGT	1 cut(s)	919
PleI	GAGTC	8 cut(s)	592, 1380, 1834, 2295, 2342, 2557, 4429, 4771
PmaCI	CACGTG	1 cut(s)	469
PmlI	CACGTG	1 cut(s)	469
PpsI	GAGTC	8 cut(s)	592, 1380, 1834, 2295, 2342, 2557, 4429, 4771
Ppu21I	YACGTR	1 cut(s)	469
PscI	ACATGT	2 cut(s)	1770, 1930
PshBI	ATTAAT	1 cut(s)	864
PsiI	TTATAA	1 cut(s)	4989
Psp124BI	GAGCTC	3 cut(s)	791, 1189, 4496
Psp1406I	AACGTT	2 cut(s)	4698, 5326
PspCI	CACGTG	1 cut(s)	469
PspEI	GGTNACC	1 cut(s)	2801
PspFI	CCCAGC	1 cut(s)	5310
PspOMI	GGGCCC	2 cut(s)	332, 4550
PstI	CTGCAG	2 cut(s)	2151, 4366
PstNI	CAGNNNCTG	7 cut(s)	524, 965, 2111, 3014, 3547, 4430, 5035
PsuI	RGATCY	4 cut(s)	1330, 2618, 4845, 4970
PvuII	CAGCTG	4 cut(s)	114, 2111, 3071, 4013
RseI	CAYNNNNRTG	9 cut(s)	575, 687, 756, 1590, 1806, 2511, 3528, 3798, 4218
SacI	GAGCTC	3 cut(s)	791, 1189, 4496
SapI	GCTCTTC	2 cut(s)	3306, 3690
ScaI	AGTACT	1 cut(s)	4819
SchI	GAGTC	8 cut(s)	593, 1381, 1834, 2296, 2342, 2557, 4430, 4772
SexAI	ACCWGGT	1 cut(s)	2089
SfcI	CTRYAG	7 cut(s)	115, 475, 1062, 2147, 2292, 4362, 4438
SfuI	TTCGAA	1 cut(s)	2731
SinI	GGWCC	7 cut(s)	455, 1546, 2073, 2967, 3538, 5026, 5276
SmiMI	CAYNNNNRTG	9 cut(s)	575, 687, 756, 1590, 1806, 2511, 3528, 3798, 4218
SmlI	CTYRAG	5 cut(s)	1298, 1629, 2953, 3488, 3614
SmoI	CTYRAG	5 cut(s)	1298, 1629, 2953, 3488, 3614
SpeI	ACTAGT	3 cut(s)	3950, 4112, 4286
SseBI	AGGCCT	2 cut(s)	2166, 5258
SsiI	CCGC	9 cut(s)	175, 3157, 3505, 3923, 4035, 4376, 4648, 4660, 5086
SspI	AATATT	2 cut(s)	1672, 4799
SstI	GAGCTC	3 cut(s)	791, 1189, 4496
StuI	AGGCCT	2 cut(s)	2166, 5258
StyI	CCWWGG	6 cut(s)	576, 1471, 2436, 3996, 4060, 4716
TaiI	ACGT	7 cut(s)	471, 1271, 1462, 2689, 4701, 4987, 5329
TaqII	GACCGA	1 cut(s)	5293
TatI	WGTACW	6 cut(s)	206, 1579, 3677, 3856, 3953, 4817
TauI	GCSGC	4 cut(s)	177, 3926, 4662, 5089
TseFI	GTSAC	4 cut(s)	4108, 4479, 4576, 4744
Tsp45I	GTSAC	4 cut(s)	4108, 4479, 4576, 4744
TspGWI	ACGGA	5 cut(s)	2241, 3592, 4504, 4769, 4954
Van91I	CCANNNNNTGG	2 cut(s)	248, 2111
Vha464I	CTTAAG	1 cut(s)	1298
VpaK11BI	GGWCC	7 cut(s)	455, 1546, 2073, 2967, 3538, 5026, 5276
VspI	ATTAAT	1 cut(s)	864
XagI	CCTNNNNNAGG	1 cut(s)	4559
XapI	RAATTY	5 cut(s)	1787, 3038, 3209, 3578, 3664
XbaI	TCTAGA	1 cut(s)	3962
XceI	RCATGY	4 cut(s)	1774, 1934, 4526, 4623
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1162
XmiI	GTMKAC	1 cut(s)	4571
XmnI	GAANNNNTTC	3 cut(s)	1438, 2634, 5327
ZrmI	AGTACT	1 cut(s)	4819
Zsp2I	ATGCAT	1 cut(s)	1361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.