Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002065

Physical Location & Seq

Genomic Coordinates Forward (+)

18238983 .. 18247288

Gene Structure 8306 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1224300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5445 bp

ATGGGCACCCCCGACAACAAACTCTCCGAGTTAGTTGACGTATTCAAGTCTTGGATTCCCAGGAGGTCCGAGCCGCCCAATGTGTCCAGGGACTTTTGGATGCCTGATCAGAGCTGTAGGGTATGTTATGACTGTGACTCTCAGTTCACAATATTTAACCGAAGGCATCATTGTCGACTTTGTGGCCGAGTTTTCTGTGCCAAGTGTACTGCAAACTCTATTCCTGCGGTGTCCGATGAGCCAAGGGTTGGAAGGGAAGATTGGGAGCGGATTAGGGTCTGTAATTATTGCTTCGAGCAATGGGAGCAAGGGGGAGCCGCACCCAATAATGCTGCCACCCCCGCAGCCAGCCCTGGTCTCAGTCCATCACAATCCGTGACAAGTTTGGCAAGCACCAAGTCCAGCTGCACCTGCCACAGCAGCAGCAGCACTATTGGTTCTACCCCTTACTCGACTGGACCTTACCAGCGTGTGCCATATAGTTCTGGTCAGAGCCCTAGTCAATCATCTTCCGAAATTTATTCAGTTCCGGTACAGCAAGACATTGTGACATCGCAGATGAGCATTAGTTCTGATGTAGCCATGGCTGAGCCGTCTCCAAATCTATATGGCTTTTGTATGAACAGTGATGATGAGGATGATGACTATGGAGTTTATCGTTTAGATTCAGAACCAAGTCATATCTCTCATGGCAATGACTATTATGGTGCAGTCACTATTGAGGAATTTGCTAGCGTCTATGGACCCCAGAATGTGCATCTTGACGGAGATAACACGAGTTCCTTATTATCTGGGAGTTTTGATACACAGGATGTAGTGGGAATTCAGAAAATTGAGGAAGAACAATATGAACACGATATCGGTGATGAATGTGGAACTTCTGCATATGACCTGCAAAGCACGAATACCGAACCTGTGGATTTCGAGAACAATGGGCTACTTTGGCTCCCTCCGGAGCCTGAGGATGAAGAAGATGAGAGAGAAGCTGTCTTATTTGATGACGACGATTATGATGGTGGTGGTGCAGGGGAGTGGGGATATCTGGGTTCTTCAAATGGCATTGGTAATGGGGAGTGTCGTACGAGGGAGAAATCAATTGAGGAGCACAGGAAGGCAATGAAGAATGTGGTGGAAGGTCATTTTAGGGCTTTGGTATCTCAGCTTTTGCAGGTTGAAAACCTACCACGGGGTGATGAAGGCAACAATGAGAGCTGGTTGGACATAATTACTTCGTTGTCTTGGGAAGCTGCCATGCTTCTCAAGCCAGATATGAGCAAGGGTGGAGGAATGGACCCTGGGGGATACGTGAAGGTTAAATGTATAGCTTGCGGGCGTCGAATTGATAGTACGGTGGTAAAAGGAGTTGTTTGTAAGAAAAATGTGGCTCATCGGCGAATGACATCACAAATAGAAAAACCACGCTTTTTAATCCTTGGAGGAGCTTTGGAATATCAGCGTGTTTCAAACCTCTTGTCAAGTTTTGATACTTTGTTGCAGCAGGAAATGGATCATCTGAAAATGGCAGTTGCAAAGATAGACTCACATCACCCTAATGTCCTTTTGGTGGAGAAGTCTGTATCTCGGTATGCTCAAGATTACCTTCTTGCAAAGGACATCTCACTTGTTCTGAATATTAAGAGGCCACTTTTAGAGCGAATAGCCCGCTGCACTGGGGCACAAATAGTCCCTTCAATAGATCATCTCACATCACCAAAACTAGGGTTTTGTGACATGTTCAATGTGAAGAAGTTTCTTGAAGTGCATGGCAGTGCTGGGCAAGGTGGGAAGAAATTGACAAAGACTCTGATGTTCTTCGAAGGTTGCCCGAAGCCCTTGGGTGTTACCATAATGCTCCACGGTGCCAATGGGGATGAGTTGAAGAAGGTGAAACATGTTGTCCAATATGGAGTTTTTGCTGCTTATCACTTGGCTCTGGAGACTTCATTTTTAGCTGATGAAGGTGCTTCACTTCCAGAACTTCCTTTGAAGTCTGCGATTAATGTTGCATTACCCGATAAACCATCAAGTGTTGACAGGTCTATTTCCACCATACCTGGTTTTAGTGTCCCACCGGCTGGCATGCCTCAGGGTCCCAATGCTAGCAGTGAGCTACAGAAATCTTTTCAAGGGTTAATTACAGATAGCAATTCATCTACCACTAGTGGCCCCATTTTGAACCTGCAAGGTGCCGATTCAATTTGCTCATCTAAAGCTTGTTCTCAAGCTTTTCTCATAGAACATGCTTCGAGCTCTAAAGAATCTAGATCTCTCTTTACTTCCTTGTCTCCTCCCCAAGAGGATATCACAGAATCCTACCATAAAGAGCTCCCCTCAATATGTGCCTCTGAAAATAAAATTGATGTGGGTTCTAAAGATTCTTGCCTGGACAATTCAGCTCAGGCTGGGGAAGCTTTGGTCAACAATGGTTTAATCTCTAATTCTCTTGCCACCTCAGAGGCATTAGGACATGGTGGCGGTGCTTTAGCTGCAACTCTTGGTGAGACTCCAGAATTGACGTCCATAAAACATCATAGTGATAATCAGAATGAGGAGGTAGGATCTTCAAAGGAAGAGTTTCTGCCCTCACCTTCTGACCACCAAAGCATTTTGGTTTCCCTGTCAACACGTTGTGTGTGGAAGGGAACTGTCTGTGAACGGTCCCATCTGTGTCGAATCAAATATTACGGAAGTTTTGATAAGCCTTTGGGGAGGTTCTTACGAGATCATCTATTTGATCAGAATTACCTTTGTCGTTCATGTGGGATGCCATCAGAAGCGCATGTTCATAGTTATACTCATAGACAAGGGAGCCTTACAATATCCGTTAAGAAGCTCCCAGAGATTTTGCTACCGGGGGAAAGGGAAGGAAAAATTTGGATGTGGCACAGGTGCTTGAAATGTCCTCGAACCAATGGCTTCCCTCCTGCAACTCGAAGAGTAGTCATGTCAGATGCTGCTTGGGGTTTGTCATTTGGGAAGTTTTTGGAGTTAAGCTTTTCAAATCATGCAGCTGCAAACAGAGTTGCAACTTGTGGTCATTCCCTTCACAGAGATTGTCTTCGGTTTTATGGTTTCGGAAGAATGGTTGCTTGCTTCAGCTATGCCTCCATTCATGTTCATTCTGTTTATCTTCCACCACAAAAACTCGAATTTCACAATGATAATCAGGAATGGATACAAAAAGAAGCAGAGGAGGTGGGTCATCGGGCAGAGCTTCTCTTTACTGAATTGCGCAATGCTCTTAATCATATTCTGGAGAAGAGACCAATTTCAGGGACAGCGGATGGTGGTAAGAAAGCACCTGAATCAAGTCAAATTGTAGAACTTGAAGAGATGCTTCAAAAGGAAAGAGAAGACTTTGAGGAATCATTACGAAAAACGATGCATAAGGAGGTGAAGTTTGGCCAGCCTGCAATTGATATTCTCGAGATCAATAGATTAAGGAGGCAGTTGCTCTTTCACTCTTACATTTGGGATCAACACCTAATTCAGGCTGCCAGTTTAAGTAAAAAAAGTTTCCAGGAAGGTTTGAGCAGTTCCCTTCCAAAACTCAAGGAGAAACCTATCAGTTCTATGGAGAAGCTTGTCAACACAAATATAAATTCCATGCCAGGTAAAGGATTAAGTAGTAGTGATTCCTCTCTTCGGGAAACAAAGCCTGATGTAAGTATTTATCAAGGAGGAGATGTTGGCAGCTTTAGCCAGCCTGGAGGGGAGCAGAACAAAAATGAAATGGTTCAGGACCCGAATCATAGTAATGAGGCAAAAAATTTCACACCTTCCTCTGACAATTCCATTGATAAATCTGATCCATTGGAATCTGGAACAAGTGTACGAAGGACCCTCTCAGAAGGGAACGAGAGTCCAGTAGTGGCGAATTTATCTGATACCCTTGATGCAGCTTGGACTGGTGAAAGTCACCCAACAAGTACCATACCTAAAGAGAATGGCTACTCTCTACCTGATTCAACCTTTGTAAATTCTGCAACTGTGTTGAGAAAAGTTGAATCAAATTCAGATTTGCAAAATTGTGCTGTTGACCAAGCTGGGGTACAGGCAACTGCTCCAACTCATTCTCTTAGCTCTAAATCTCTCAAGGTGTTTGACAAAAATTATTCCTTTAATTCTCAAAAGATCATCATTGGTGAATACAATCCCGTCAATGTTCCAATGTTTCGGGAATTGGAAAGGCAAAGCCGTGCCAGACTGTTACTGCCCATTGGTGTTAATGACACTGTCATCCCTGTTTTTGACGATGAACCCACTAGTGTTATTGCATATGCACTTGTCTCGTCAGATTACCATTTGCAAATTTCCGAGTCTGAGAGACCAAGAGATGCTCTGGATAGTTCAGTCTCATTACCTCTTTTTGATTCCGCAAATCTTCTCTCACTTAGCTCTTTTGATGAGTCGCTTTCTGAGACCTACAGAAATCTTGGTTCTTCTGATGAGGGCATGTTATCTGTGTCACGGTCGCGGAGCTCTCAAGCTTTGGACTCACTTTTGTCAAAGGACATTCATGCTAGAGTTTCTTTTACAGATGGTGGCACCCTTGGGAAGGTGAAGTATACAGTGACATGTTACTACGCAACGCGGTTTGAGGCTTTGAGGAGGACGTGTTGCCCTTCTGAAATAGATTTCGTACGATCTCTTAGCCGTTGTAAGAAGTGGGGCGCCCAGGGTGGCAAGAGCAATGTTTTCTTTGCAAAAACCTTGGATGATCGGTTTATCATTAAACAAGTTACAAAGACAGAGCTGGAATCATTTATCAAGTTTGCACCGTCTTATTTCAAGTATTTGTCCGAGTCAATCAGTACTAGAAGCCCAACTTGCCTGGCAAAGATCCTGGGTATTTATCAGGTTTCGTCAAAGCATGGTAAAGCAGGGAAAGAGTCCAAGATGGATGTTTTAGTAATGGAGAATTTATTATTTAGACGGAATGTCACGCGGCTTTATGATCTGAAAGGATCTTCTCGATCACGTTATAATCCAGATACAAGTGGGAGCAATAAAGTTCTACTCGACCAGAACCTGATTGAAGCAATGCCAACTTCTCCAATTTTTGTGGGGAGCAAGGCAAAGCGGCTACTAGAGAGGGCTGTCTGGAATGATACTGCGTTCCTTGCTTCGATAGATGTCATGGACTACTCGTTACTGGTCGGCGTGGATGAGGAAAAGGATGAGCTAGTTCTGGGGATCATAGATTTTATGAGGCAATACACATGGGACAAGCACCTTGAAACTTGGGTGAAGACCTCAGGAATTCTCGGCGGAGCCAAGAACACGTCTCCAACCGTGATTTCTCCCCAGCAATACAAGAAACGATTCAGGAAAGCTATGACAACTTATTTCCTCATGGTACCCGATCAGTGGTCTCCCCTAACGATCGTTCCAAGCGGGTCGCAGTCTGACGTTGGTGAAGAAAATGCGCAAGATCCGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0008150 GO:0008152 GO:0008289 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1815

Amino Acids

200.25

Weight (kDa)

5.66

Isoelectric Point (pI)

51.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	2.4e-17	FYVE zinc finger
Cpn60_TCP1	PF00118	404 - 651	1.5e-28	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1560 - 1726	1.2e-34	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1726 - 1782	2.7e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240 AT4G33240
fragaria_vesca	FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530 FvH4_1g09530
malus_domestica	MD02G1103900.v1.1 MD15G1224300.v1.1
prunus_persica	Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1 Prupe.7G191000_v2.0.a1
pyrus_communis	pycom02g08170 pycom15g19930 pycom15g19940
rosa_chinensis	RchiOBHm_Chr2g0096101
rosa_laevigata	RLG00000016625
rosa_multiflora	Rmu_sc0005996.1_g000006
rosa_roxburghii	Rroxscaffold_2G00145750
rosa_rugosa	Rorug02G0057000 Rorug02G0057100 Rorug02G0057200 Rorug02G0057200
rosa_samantha	Rh2AG104000 Rh2BG106100 Rh2CG108000 Rh2DG106700
rosa_wichuraiana	Rw2G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4986
AarI	CACCTGC	1 cut(s)	419
AatII	GACGTC	1 cut(s)	2519
Acc16I	TGCGCA	2 cut(s)	3233, 5430
Acc36I	ACCTGC	4 cut(s)	419, 900, 1159, 2187
Acc65I	GGTACC	1 cut(s)	5359
AccB1I	GGYRCC	6 cut(s)	5, 1859, 2186, 4547, 4673, 5359
AccB7I	CCANNNNNTGG	2 cut(s)	248, 2075
AccBSI	CCGCTC	1 cut(s)	268
AccI	GTMKAC	2 cut(s)	175, 4568
AccII	CGCG	3 cut(s)	4477, 4594, 4948
AccIII	TCCGGA	1 cut(s)	952
AclWI	GGATC	8 cut(s)	1515, 2566, 3483, 3803, 4837, 4975, 5204, 5429
AcoI	YGGCCR	2 cut(s)	184, 3403
AcuI	CTGAAG	1 cut(s)	3079
AcyI	GRCGYC	3 cut(s)	1333, 2516, 4674
AdeI	CACNNNGTG	2 cut(s)	1190, 2630
AflIII	ACRYGT	6 cut(s)	1731, 1891, 2624, 4577, 4616, 5283
AhdI	GACNNNNNGTC	1 cut(s)	4940
AhlI	ACTAGT	2 cut(s)	2159, 4265
AjiI	CACGTC	2 cut(s)	4617, 5286
AjuI	GAANNNNNNNTTGG	4 cut(s)	244, 276, 5284, 5316
Alw21I	GWGCWC	4 cut(s)	1107, 2252, 2328, 4484
AlwI	GGATC	8 cut(s)	1515, 2566, 3483, 3803, 4837, 4975, 5204, 5429
AlwNI	CAGNNNCTG	2 cut(s)	2954, 5032
Ama87I	CYCGRG	1 cut(s)	3425
Aor13HI	TCCGGA	1 cut(s)	952
AoxI	GGCC	4 cut(s)	184, 1640, 2164, 3403
ArsI	GACNNNNNNTTYG	2 cut(s)	1457, 1489
AseI	ATTAAT	1 cut(s)	1998
Asp700I	GAANNNNTTC	3 cut(s)	2574, 3735, 5324
Asp718I	GGTACC	1 cut(s)	5359
AspLEI	GCGC	4 cut(s)	2779, 3234, 4676, 5431
AspS9I	GGNCC	9 cut(s)	66, 458, 743, 1291, 2090, 2165, 2658, 3742, 3840
AsuC2I	CCSGG	1 cut(s)	2853
AsuII	TTCGAA	1 cut(s)	1815
AsuNHI	GCTAGC	2 cut(s)	731, 2099
AvaI	CYCGRG	1 cut(s)	3425
AvaII	GGWCC	8 cut(s)	66, 458, 743, 1291, 2090, 2658, 3742, 3840
AxyI	CCTNAGG	3 cut(s)	960, 2085, 5257
BaeGI	GKGCMC	2 cut(s)	8, 1678
BalI	TGGCCA	1 cut(s)	3405
BanI	GGYRCC	6 cut(s)	5, 1859, 2186, 4547, 4673, 5359
BanII	GRGCYC	4 cut(s)	497, 2252, 2328, 4484
BauI	CACGAG	1 cut(s)	775
BbsI	GAAGAC	3 cut(s)	3050, 3360, 5258
Bbv12I	GWGCWC	4 cut(s)	1107, 2252, 2328, 4484
BccI	CCATC	8 cut(s)	373, 1007, 2029, 2670, 2776, 3278, 4535, 4894
BceAI	ACGGC	3 cut(s)	577, 4182, 4641
BcgI	CGANNNNNNTGC	4 cut(s)	155, 189, 4272, 4306
BciVI	GTATCC	2 cut(s)	1295, 3168
BclI	TGATCA	2 cut(s)	106, 2734
BcnI	CCSGG	1 cut(s)	2853
BcuI	ACTAGT	2 cut(s)	2159, 4265
BfmI	CTRYAG	3 cut(s)	115, 2111, 4426
BfoI	RGCGCY	1 cut(s)	4677
BfuAI	ACCTGC	4 cut(s)	419, 900, 1159, 2187
BfuI	GTATCC	2 cut(s)	1295, 3168
BglII	AGATCT	1 cut(s)	2264
BlpI	GCTNAGC	1 cut(s)	588
BmcAI	AGTACT	1 cut(s)	4816
Bme18I	GGWCC	8 cut(s)	66, 458, 743, 1291, 2090, 2658, 3742, 3840
BmeRI	GACNNNNNGTC	1 cut(s)	4940
BmeT110I	CYCGRG	1 cut(s)	3425
BmgBI	CACGTC	2 cut(s)	4617, 5286
BmgT120I	GGNCC	9 cut(s)	66, 458, 743, 1291, 2090, 2165, 2658, 3742, 3840
BmrI	ACTGGG	1 cut(s)	1680
BmsI	GCATC	9 cut(s)	90, 175, 766, 2754, 2941, 3324, 3372, 3886, 4327
BmtI	GCTAGC	2 cut(s)	735, 2103
BmuI	ACTGGG	1 cut(s)	1680
BpiI	GAAGAC	3 cut(s)	3050, 3360, 5258
BplI	GAGNNNNNCTC	2 cut(s)	2315, 2347
BpmI	CTGGAG	4 cut(s)	1955, 2490, 3275, 3729
Bpu10I	CCTNAGC	1 cut(s)	2397
Bpu1102I	GCTNAGC	1 cut(s)	588
Bpu14I	TTCGAA	1 cut(s)	1815
BpuEI	CTTGAG	6 cut(s)	1244, 1575, 2205, 3536, 4079, 4470
BpuMI	CCSGG	1 cut(s)	2853
BsaAI	YACGTR	1 cut(s)	1306
BsaHI	GRCGYC	3 cut(s)	1333, 2516, 4674
BsaI	GGTCTC	5 cut(s)	362, 3256, 4321, 4415, 5379
BsaWI	WCCGGW	2 cut(s)	529, 952
Bse118I	RCCGGY	1 cut(s)	2071
Bse1I	ACTGG	6 cut(s)	460, 1675, 3498, 3866, 3913, 5160
Bse21I	CCTNAGG	3 cut(s)	960, 2085, 5257
Bse3DI	GCAATG	6 cut(s)	305, 700, 1122, 3241, 4699, 5049
BseAI	TCCGGA	1 cut(s)	952
BseMI	GCAATG	6 cut(s)	305, 700, 1122, 3241, 4699, 5049
BseNI	ACTGG	6 cut(s)	460, 1675, 3498, 3866, 3913, 5160
BseRI	GAGGAG	7 cut(s)	1115, 1452, 2277, 2564, 3206, 3696, 4624
BseSI	GKGCMC	2 cut(s)	8, 1678
BseYI	CCCAGC	4 cut(s)	1772, 2402, 4046, 5307
BsgI	GTGCAG	4 cut(s)	391, 729, 1044, 1651
Bsh1236I	CGCG	3 cut(s)	4477, 4594, 4948
Bsh1285I	CGRYCG	2 cut(s)	4475, 5388
BshFI	GGCC	4 cut(s)	186, 1642, 2166, 3405
BshNI	GGYRCC	6 cut(s)	5, 1859, 2186, 4547, 4673, 5359
BsiEI	CGRYCG	2 cut(s)	4475, 5388
BsiHKAI	GWGCWC	4 cut(s)	1107, 2252, 2328, 4484
BsiHKCI	CYCGRG	1 cut(s)	3425
BsiSI	CCGG	4 cut(s)	530, 953, 2072, 2852
BsiWI	CGTACG	2 cut(s)	1079, 4642
BslFI	GGGAC	7 cut(s)	104, 1670, 2051, 2076, 2644, 3289, 5240
BsmBI	CGTCTC	2 cut(s)	600, 5292
BsmFI	GGGAC	7 cut(s)	104, 1670, 2051, 2076, 2644, 3289, 5240
BsnI	GGCC	4 cut(s)	186, 1642, 2166, 3405
Bso31I	GGTCTC	5 cut(s)	362, 3256, 4321, 4415, 5379
BsoBI	CYCGRG	1 cut(s)	3425
Bsp119I	TTCGAA	1 cut(s)	1815
Bsp1286I	GDGCHC	7 cut(s)	8, 497, 1107, 1678, 2252, 2328, 4484
Bsp13I	TCCGGA	1 cut(s)	952
Bsp1720I	GCTNAGC	1 cut(s)	588
Bsp19I	CCATGG	1 cut(s)	582
BspANI	GGCC	4 cut(s)	186, 1642, 2166, 3405
BspEI	TCCGGA	1 cut(s)	952
BspFNI	CGCG	3 cut(s)	4477, 4594, 4948
BspMI	ACCTGC	4 cut(s)	419, 900, 1159, 2187
BspOI	GCTAGC	2 cut(s)	735, 2103
BspPI	GGATC	8 cut(s)	1515, 2566, 3483, 3803, 4837, 4975, 5204, 5429
BspT104I	TTCGAA	1 cut(s)	1815
BspT107I	GGYRCC	6 cut(s)	5, 1859, 2186, 4547, 4673, 5359
BspTNI	GGTCTC	5 cut(s)	362, 3256, 4321, 4415, 5379
BsrBI	CCGCTC	1 cut(s)	268
BsrDI	GCAATG	6 cut(s)	305, 700, 1122, 3241, 4699, 5049
BsrFI	RCCGGY	1 cut(s)	2071
BsrI	ACTGG	6 cut(s)	460, 1675, 3498, 3866, 3913, 5160
BssAI	RCCGGY	1 cut(s)	2071
BssNAI	GTATAC	1 cut(s)	4569
BssNI	GRCGYC	3 cut(s)	1333, 2516, 4674
BssSI	CACGAG	1 cut(s)	775
BssT1I	CCWWGG	6 cut(s)	242, 582, 1432, 1833, 4552, 4713
Bst1107I	GTATAC	1 cut(s)	4569
Bst2BI	CACGAG	1 cut(s)	775
Bst6I	CTCTTC	5 cut(s)	2565, 2929, 3254, 3324, 3648
BstACI	GRCGYC	3 cut(s)	1333, 2516, 4674
BstBAI	YACGTR	1 cut(s)	1306
BstBI	TTCGAA	1 cut(s)	1815
BstDSI	CCRYGG	3 cut(s)	582, 1184, 1855
BstENI	CCTNNNNNAGG	2 cut(s)	3488, 4556
BstFNI	CGCG	3 cut(s)	4477, 4594, 4948
BstH2I	RGCGCY	1 cut(s)	4677
BstHHI	GCGC	4 cut(s)	2779, 3234, 4676, 5431
BstMCI	CGRYCG	2 cut(s)	4475, 5388
BstNSI	RCATGY	7 cut(s)	1735, 1895, 2083, 2243, 2783, 4459, 4581
BstSFI	CTRYAG	3 cut(s)	115, 2111, 4426
BstSLI	GKGCMC	2 cut(s)	8, 1678
BstUI	CGCG	3 cut(s)	4477, 4594, 4948
BstV2I	GAAGAC	3 cut(s)	3050, 3360, 5258
BstX2I	RGATCY	5 cut(s)	2264, 2558, 4842, 4967, 5434
BstYI	RGATCY	5 cut(s)	2264, 2558, 4842, 4967, 5434
BstZ17I	GTATAC	1 cut(s)	4569
Bsu36I	CCTNAGG	3 cut(s)	960, 2085, 5257
BsuI	GTATCC	2 cut(s)	1295, 3168
BsuRI	GGCC	4 cut(s)	186, 1642, 2166, 3405
BtgI	CCRYGG	3 cut(s)	582, 1184, 1855
BtgZI	GCGATG	1 cut(s)	537
BtrI	CACGTC	2 cut(s)	4617, 5286
BtsI	GCAGTG	2 cut(s)	1774, 2110
BtsIMutI	CAGTG	7 cut(s)	631, 1668, 1774, 2110, 4233, 4578, 5375
BveI	ACCTGC	4 cut(s)	419, 900, 1159, 2187
CaiI	CAGNNNCTG	2 cut(s)	2954, 5032
CfoI	GCGC	4 cut(s)	2779, 3234, 4676, 5431
Cfr10I	RCCGGY	1 cut(s)	2071
Cfr13I	GGNCC	9 cut(s)	66, 458, 743, 1291, 2090, 2165, 2658, 3742, 3840
CseI	GACGC	2 cut(s)	724, 1322
CsiI	ACCWGGT	1 cut(s)	2053
CspCI	CAANNNNNGTGG	2 cut(s)	5046, 5081
DinI	GGCGCC	1 cut(s)	4675
DraIII	CACNNNGTG	2 cut(s)	1190, 2630
DriI	GACNNNNNGTC	1 cut(s)	4940
EaeI	YGGCCR	2 cut(s)	184, 3403
Eam1104I	CTCTTC	5 cut(s)	2565, 2929, 3254, 3324, 3648
Eam1105I	GACNNNNNGTC	1 cut(s)	4940
EarI	CTCTTC	5 cut(s)	2565, 2929, 3254, 3324, 3648
EciI	GGCGGA	1 cut(s)	5286
Ecl136II	GAGCTC	3 cut(s)	2250, 2326, 4482
Eco130I	CCWWGG	6 cut(s)	242, 582, 1432, 1833, 4552, 4713
Eco24I	GRGCYC	4 cut(s)	497, 2252, 2328, 4484
Eco31I	GGTCTC	5 cut(s)	362, 3256, 4321, 4415, 5379
Eco32I	GATATC	3 cut(s)	859, 1040, 2302
Eco47I	GGWCC	8 cut(s)	66, 458, 743, 1291, 2090, 2658, 3742, 3840
Eco53kI	GAGCTC	3 cut(s)	2250, 2326, 4482
Eco57I	CTGAAG	1 cut(s)	3079
Eco81I	CCTNAGG	3 cut(s)	960, 2085, 5257
Eco88I	CYCGRG	1 cut(s)	3425
EcoICRI	GAGCTC	3 cut(s)	2250, 2326, 4482
EcoNI	CCTNNNNNAGG	2 cut(s)	3488, 4556
EcoO109I	RGGNCCY	3 cut(s)	2090, 3742, 3840
EcoRI	GAATTC	2 cut(s)	822, 5262
EcoRV	GATATC	3 cut(s)	859, 1040, 2302
EcoT14I	CCWWGG	6 cut(s)	242, 582, 1432, 1833, 4552, 4713
EcoT22I	ATGCAT	1 cut(s)	3387
EcoT38I	GRGCYC	4 cut(s)	497, 2252, 2328, 4484
EgeI	GGCGCC	1 cut(s)	4675
EheI	GGCGCC	1 cut(s)	4675
ErhI	CCWWGG	6 cut(s)	242, 582, 1432, 1833, 4552, 4713
Esp3I	CGTCTC	2 cut(s)	600, 5292
FalI	AAGNNNNNCTT	8 cut(s)	1584, 1616, 2796, 2828, 3321, 3353, 4813, 4845
FaqI	GGGAC	7 cut(s)	104, 1670, 2051, 2076, 2644, 3289, 5240
FauI	CCCGC	4 cut(s)	349, 1322, 1670, 5390
FauNDI	CATATG	2 cut(s)	886, 4279
FbaI	TGATCA	2 cut(s)	106, 2734
FblI	GTMKAC	2 cut(s)	175, 4568
FriOI	GRGCYC	4 cut(s)	497, 2252, 2328, 4484
FspI	TGCGCA	2 cut(s)	3233, 5430
GlaI	GCGC	4 cut(s)	2778, 3233, 4675, 5430
GsaI	CCCAGC	4 cut(s)	1776, 2406, 4050, 5311
GsuI	CTGGAG	4 cut(s)	1955, 2490, 3275, 3729
HaeII	RGCGCY	1 cut(s)	4677
HaeIII	GGCC	4 cut(s)	186, 1642, 2166, 3405
HapII	CCGG	4 cut(s)	530, 953, 2072, 2852
HgaI	GACGC	2 cut(s)	724, 1322
HhaI	GCGC	4 cut(s)	2779, 3234, 4676, 5431
Hin1I	GRCGYC	3 cut(s)	1333, 2516, 4674
Hin6I	GCGC	4 cut(s)	2777, 3232, 4674, 5429
HinP1I	GCGC	4 cut(s)	2777, 3232, 4674, 5429
HincII	GTYRAC	7 cut(s)	37, 176, 2032, 2419, 2622, 3589, 4039
HindII	GTYRAC	7 cut(s)	37, 176, 2032, 2419, 2622, 3589, 4039
HindIII	AAGCTT	6 cut(s)	2211, 2223, 2409, 2992, 3581, 4488
HpaII	CCGG	4 cut(s)	530, 953, 2072, 2852
Hpy99I	CGWCG	2 cut(s)	1007, 1338
HpyCH4IV	ACGT	8 cut(s)	39, 1305, 2516, 2626, 4616, 4981, 5285, 5412
HpySE526I	ACGT	8 cut(s)	39, 1305, 2516, 2626, 4616, 4981, 5285, 5412
Hsp92I	GRCGYC	3 cut(s)	1333, 2516, 4674
HspAI	GCGC	4 cut(s)	2777, 3232, 4674, 5429
KasI	GGCGCC	1 cut(s)	4673
KflI	GGGWCCC	1 cut(s)	2090
Kpn2I	TCCGGA	1 cut(s)	952
KpnI	GGTACC	1 cut(s)	5363
Ksp22I	TGATCA	2 cut(s)	106, 2734
LweI	GCATC	9 cut(s)	90, 175, 766, 2754, 2941, 3324, 3372, 3886, 4327
MabI	ACCWGGT	1 cut(s)	2053
MaeII	ACGT	8 cut(s)	39, 1305, 2516, 2626, 4616, 4981, 5285, 5412
MbiI	CCGCTC	1 cut(s)	268
MfeI	CAATTG	2 cut(s)	1095, 3414
MflI	RGATCY	5 cut(s)	2264, 2558, 4842, 4967, 5434
MhlI	GDGCHC	7 cut(s)	8, 497, 1107, 1678, 2252, 2328, 4484
MlsI	TGGCCA	1 cut(s)	3405
MluNI	TGGCCA	1 cut(s)	3405
Mly113I	GGCGCC	1 cut(s)	4674
MmeI	TCCRAC	4 cut(s)	229, 1197, 4091, 5315
Mox20I	TGGCCA	1 cut(s)	3405
Mph1103I	ATGCAT	1 cut(s)	3387
MroI	TCCGGA	1 cut(s)	952
MroXI	GAANNNNTTC	3 cut(s)	2574, 3735, 5324
MscI	TGGCCA	1 cut(s)	3405
MslI	CAYNNNNRTG	5 cut(s)	693, 1551, 1767, 2532, 3756
Msp20I	TGGCCA	1 cut(s)	3405
MspA1I	CMGCKG	4 cut(s)	405, 1665, 3011, 3281
MspI	CCGG	4 cut(s)	530, 953, 2072, 2852
MunI	CAATTG	2 cut(s)	1095, 3414
MvnI	CGCG	3 cut(s)	4477, 4594, 4948
NarI	GGCGCC	1 cut(s)	4674
NciI	CCSGG	1 cut(s)	2853
NcoI	CCATGG	1 cut(s)	582
NdeI	CATATG	2 cut(s)	886, 4279
NheI	GCTAGC	2 cut(s)	731, 2099
NmeAIII	GCCGAG	2 cut(s)	212, 5247
NmuCI	GTSAC	9 cut(s)	134, 376, 547, 712, 1727, 3917, 4467, 4573, 4942
NsbI	TGCGCA	2 cut(s)	3233, 5430
NsiI	ATGCAT	1 cut(s)	3387
NspI	RCATGY	7 cut(s)	1735, 1895, 2083, 2243, 2783, 4459, 4581
NspV	TTCGAA	1 cut(s)	1815
PaeI	GCATGC	1 cut(s)	2083
PaeR7I	CTCGAG	1 cut(s)	3425
PaqCI	CACCTGC	1 cut(s)	419
PasI	CCCWGGG	2 cut(s)	1295, 4678
PciI	ACATGT	3 cut(s)	1731, 1891, 4577
PdmI	GAANNNNTTC	3 cut(s)	2574, 3735, 5324
Pfl23II	CGTACG	2 cut(s)	1079, 4642
PflFI	GACNNNGTC	1 cut(s)	4235
PflMI	CCANNNNNTGG	2 cut(s)	248, 2075
PfoI	TCCNGGA	1 cut(s)	3519
Ple19I	CGATCG	1 cut(s)	5388
PluTI	GGCGCC	1 cut(s)	4677
Ppu21I	YACGTR	1 cut(s)	1306
PpuMI	RGGWCCY	3 cut(s)	2090, 3742, 3840
PscI	ACATGT	3 cut(s)	1731, 1891, 4577
PshBI	ATTAAT	1 cut(s)	1998
PsiI	TTATAA	1 cut(s)	4986
Psp124BI	GAGCTC	3 cut(s)	2252, 2328, 4484
Psp5II	RGGWCCY	3 cut(s)	2090, 3742, 3840
PspFI	CCCAGC	4 cut(s)	1772, 2402, 4046, 5307
PspLI	CGTACG	2 cut(s)	1079, 4642
PspPI	GGNCC	9 cut(s)	66, 458, 743, 1291, 2090, 2165, 2658, 3742, 3840
PspPPI	RGGWCCY	3 cut(s)	2090, 3742, 3840
PstNI	CAGNNNCTG	2 cut(s)	2954, 5032
PsuI	RGATCY	5 cut(s)	2264, 2558, 4842, 4967, 5434
PsyI	GACNNNGTC	1 cut(s)	4235
PvuI	CGATCG	1 cut(s)	5388
PvuII	CAGCTG	2 cut(s)	405, 3011
RseI	CAYNNNNRTG	5 cut(s)	693, 1551, 1767, 2532, 3756
SacI	GAGCTC	3 cut(s)	2252, 2328, 4484
SalI	GTCGAC	1 cut(s)	174
Sau96I	GGNCC	9 cut(s)	66, 458, 743, 1291, 2090, 2165, 2658, 3742, 3840
ScaI	AGTACT	1 cut(s)	4816
SduI	GDGCHC	7 cut(s)	8, 497, 1107, 1678, 2252, 2328, 4484
SexAI	ACCWGGT	1 cut(s)	2053
SfaNI	GCATC	9 cut(s)	90, 175, 766, 2754, 2941, 3324, 3372, 3886, 4327
SfcI	CTRYAG	3 cut(s)	115, 2111, 4426
SfoI	GGCGCC	1 cut(s)	4675
Sfr274I	CTCGAG	1 cut(s)	3425
SfuI	TTCGAA	1 cut(s)	1815
SinI	GGWCC	8 cut(s)	66, 458, 743, 1291, 2090, 2658, 3742, 3840
SlaI	CTCGAG	1 cut(s)	3425
SmiMI	CAYNNNNRTG	5 cut(s)	693, 1551, 1767, 2532, 3756
SmlI	CTYRAG	7 cut(s)	1259, 1590, 2220, 3425, 3551, 4094, 4485
SmoI	CTYRAG	7 cut(s)	1259, 1590, 2220, 3425, 3551, 4094, 4485
SpeI	ACTAGT	2 cut(s)	2159, 4265
SphI	GCATGC	1 cut(s)	2083
SspDI	GGCGCC	1 cut(s)	4673
SspI	AATATT	3 cut(s)	153, 1633, 2681
SstI	GAGCTC	3 cut(s)	2252, 2328, 4484
StyI	CCWWGG	6 cut(s)	242, 582, 1432, 1833, 4552, 4713
TaiI	ACGT	8 cut(s)	42, 1308, 2519, 2629, 4619, 4984, 5288, 5415
TatI	WGTACW	2 cut(s)	206, 4814
TauI	GCSGC	4 cut(s)	76, 320, 4951, 5086
TscAI	CASTG	7 cut(s)	631, 1675, 1774, 2110, 4240, 4578, 5375
TseFI	GTSAC	9 cut(s)	134, 376, 547, 712, 1727, 3917, 4467, 4573, 4942
Tsp45I	GTSAC	9 cut(s)	134, 376, 547, 712, 1727, 3917, 4467, 4573, 4942
TspGWI	ACGGA	5 cut(s)	364, 780, 2700, 2812, 4951
TspRI	CASTG	7 cut(s)	631, 1675, 1774, 2110, 4240, 4578, 5375
Tth111I	GACNNNGTC	1 cut(s)	4235
Van91I	CCANNNNNTGG	2 cut(s)	248, 2075
VpaK11BI	GGWCC	8 cut(s)	66, 458, 743, 1291, 2090, 2658, 3742, 3840
VspI	ATTAAT	1 cut(s)	1998
XagI	CCTNNNNNAGG	2 cut(s)	3488, 4556
XbaI	TCTAGA	1 cut(s)	2261
XceI	RCATGY	7 cut(s)	1735, 1895, 2083, 2243, 2783, 4459, 4581
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1862
XhoI	CTCGAG	1 cut(s)	3425
XmiI	GTMKAC	2 cut(s)	175, 4568
XmnI	GAANNNNTTC	3 cut(s)	2574, 3735, 5324
ZraI	GACGTC	1 cut(s)	2517
ZrmI	AGTACT	1 cut(s)	4816
Zsp2I	ATGCAT	1 cut(s)	3387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.