MD02G1184600.v1.1

Condensin II non structural maintenance of chromosomes subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006247
Physical Location & Seq
Forward (+)
16732196 .. 16737739
5544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1184600.v1.1.491

Sequence Viewer

Length: 3771 bp
ATGGATAAGCGGCTCCGCTCATCCCTTCAGACCTCCGCCGACGAATTCCTGAGCTCCGCCGCAAAGCAAACCCTAAAATCCTCAAAGCCCTCTCTCAAAACCCTAATCCACGGCGTCAAGCCTTCCTCCGACCTCTCCTCCTCTCTCCCTCTCTCTCTGTCCCACTCCATTTCCCAAGCTATACAATCCTTCAAGAACCTTCTGGAGCCCAACCAAAACCCTAAACCTAAACCTAACTCCCCGCGCTCTCCTCCCACCAAACGGCCCCGGAGGTCTTCTAGGCGGTCCAAGACCCGACCAGAACCTGATTCAGACGACCCAGACCCTAACCCGGAGCGAGAGAGGAAATGCCTTCTTTCGGAACTTCAAGTTTTAGCATTTATCGCGCAATTGTGCATTTCGCATCCCCAGAACGCATTCTCGCCGTTGGATTTGTTGACCGGAGTTCAGGCGCTGCACGACAATCTGATTATTTTGGAGTCGGACTCGGTGCTGGTTTCGGAGATCGCGGGTTTGTGCGAGCAATGGTGGAAGGAGGAGTTGCCGAGTCGAGAATCACTGATTTCTCAGTCGCTCCCTTTCTTGCTCTCGAGATCTTTGACTCTGCAAAAGAAGGCAGATGTTCACAGAGTCTATGCGCTCCGGGAGGCCTTTACTTGCTTTGATTTCGAAGACGAGAGCATTGAGGACTTGAAGCTCTTGTTAATTCGGTGTGTGATTTCGCCGCTGTATCTGAAAGCAGAGGAGGGGAAGAAGTTCCTTGCATTTATGTTCGGGTTGAGTTTGCAGCTGTTGAAGGAAGTTTTGGCAATGATTCGATCTCAGGTTCCGTTTGGTCGAAAGTCAATGCTGGAGGCCTACGGGGACGTCCTGTTTCGGGCTTGGAAAGTGGCGGATGGCGAATCGAGAAGTGAGATCGAGAATGGGTTTTTGCAGGGTTTGATTGAGGGTGCAATACATGCCAGTTCTGGAGCTTTTGCTGCTTCGATTAGAAGGGTTTTGGGAGGGTTTATAAACCAGAGGACCACTGCTGGTGTTGAGAAGCTTCTTTTCCAGCTGTCTGAACCTGTGATATTTCGCTCGCTGCAGGTTGCGAATTCTAATGTTCGTCAAAATGCGCTGCATTTGCTGCTGGACTTGTTTCCTCTTGAAGATCCCGATTCCACAAAAGAAGTGAAAGATTCCTTGCTTGATAAGCAGTTCTTTTTATTGGAGAGATTACTCACCGATGATTGTCCAGACGTGAGAGTGGTTGCGGTGGAAGGGTCTTGTCGAGTGCTTCATCTATTTTGGGAAATCATCCCTTTGAGTACAATCACAAAGCTAATTACCAAAATTGTTGATGATATGTCGCACGATGTATGTCATGAGGTTAGACTTTCCACACTGAATGGAATTATCTACTTGTTTGGAAATCCACAATGCCATCAAATTCTCAACGTTCTTCTGCCCAGACTGGGTCATTTGATGCTGGACAATGTCCTATCAGTAAGAGCTGCAATGGCAGATCTCCTCCTTGTTATAAGGGATATTCCAAATTTCCAGTTCCATAAGGTGGTGAAATTAGATGTATTATTGTCGGACCTTGCAAATGATCAACCTCAAGTTGCTCAGAAAATTACACAACTGCTTATGCCATCATACTTTCCTTCAAAAGTGTCCGTTGAAGCAGCATGTAATCGTTGTGCTACGCTCGTTAAAAGGTCTCCAATGGCTGGAGCCAGGTTTTGTGAATTTGCTGTGTCCCAAGGGTCATCTCTCAAGTCTCTGATGGAACTTGTTCGAGTATTGATTACTTTGGTCTTGCTGCCTAGTAATCTAGATGAAGATCAAATTGAAGGTTTACTTCTTGCTGCTGCCAACCTTTGCAATACCCTAGCAAGCGAGTCAGTTTTCAAGAATGCTCTTAAGGAGTTCTTCGATGGTGAAAAGTTGAAGTGCTTGTTTGCTGCTGCATCTACTCGGTCTGCTCAGTCTTCTGTATTCAACATTGTCTCAACTGTCTCTCCAGATGATCTAGCTGGACTTCTTGACGAATGCATGAACCTGGTCACTAATTGTAGTGGTTTATCCAAAGATGCCAAGAGGCAAGCTGAAGTGAGGTCTGCTCACAAATTGTTGCTGTCATGTGATGGGGTTGATGGTATGCTTGAAGCTCTAACAGCATTCCTGCAGAAAGCTGCCTATCGTTGCCATGTCAAATTTGGCAATGAAATACCAAATCTGAGTGTTTTCTCTGCAAAAAGAAAGAAATCAAAGTCCTCTAGCAAAATTTCAGCTCGATGGAGAAATGTTGGCGGGAAAAAACCAACCAATTTTGAGAACGATTATTCAGTGGCAGTAGGAATGGCCTGGCAAATTAGAGATTTACTTGCATCTGACAACACTCGGAATTCTATATTGGGGTCTCAAGTTCTAGAGTCGTTATTTCAATCCTTAAATGTCATTTCGGAGGTCAGCATTGTGCAGTGCATGCATTGTGAATATATGGACACATCTCCTGTCTTGGCATATACAGCTCTTGCTCTGCATATGTCTCTTCAAAATGCCAGTTCGACTACAACAGATTGCGGTACAAAAAGTAAAGATAGGACTGATTCCACAAGATCTAGTTCTGAGCAGACTAAGTTGGAACATGCATTGGATCACCTAGTTAACTGTGCAGAGAAGCTATTTCGGGCAGATGATGCTGGAGGGTCCGGAAAAATTTCTCCACAACCTAAGCATTCTAGCAAGAAGTCTTCCAACCGTCGTGAGAAAAGACAGAGAGAATCGCATACTGATGCCTCAAGTCCAAGTGATAGTGGATGCGACTATACAGAACCAAAGAAGTTATCAAACAAGGTGAAGATGCTAACTGCAGTCTTAAGGTTCGTGGTTGATGCCGCTACAATAGGTTCTGTTCATCAAATTCAGGAAAGGTGCTTAAAGTTCACATCAGGTTACATAAGATATGTCGTATCTAAGTTGGAGCAACAATCTCGTGAGCCTTTTCAATTTGAGGAGGAGGATTTGAAGGATATGGTTCTCTGTTTGAAGAGCTCCTTCACATATGCAGCCAAGTTACTGAATCTAGCTCTTAAAGATGTTACTGAAGCTTCACCAGCACTCACTGAAGCGTCTGATCTTGCCAACGATCTGCTTGATTTAATCATCTCGATTGAGTTGTACTTGGGTTCTAACTATGTTGCTCATTTGGTGACTGGAGTAAAGTCTTGGCTTCCTGACTTGATTCTAGCTTTAGGATCTGGGCACATTGTAGAACAGATTAAGGGAGAAGTGATGGAAATAACTACAGCTGACCGCATCAGACTCCACTTTCCATCATGGCTTCTGATTTTAGCTAAAACTGAGCTCTCTGAAATAAGTGAAGCTAGGCCAGAAGATGGAGATTCCGAACCAGATGAGTTTCCTTCTTTTAAGAAACTTCTTAACATGATTGTTACATTGTTGAAAGGAAATATCAGCATAAGGAATGTGTTTGGGGTGATTTTCTTGATTAGCGCGATTGTTGGGATGCAAAAGAAGAATTTCGGATTGGTTTTGGGACTCGTACGCTTTGCATGCCGGAAGGTATTCAGGGAAGACGAGAGAGAGTGGGGCAATATGATGTTGGCTTCTCTTCAAGAGCTTTACCCTCAGATTGAGAGAGAAATAGAAGAAGACATTGAAGAAGACAGAAGGGAAAATCTGCGAAATGCAAAGGAATTGCTTGAACCAATTTGGATGTATCATATGTATGAAACTGGGAAGGTTTCTATGGAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Condensin2nSMC

Protein Analysis

1257

Amino Acids

140.1

Weight (kDa)

6.29

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Condensin2nSMC PF12422 237 - 385 4.2e-55 Condensin II non structural maintenance of chromosomes subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1013, 1523
AatII GACGTC 1 cut(s) 870
Acc36I ACCTGC 1 cut(s) 1078
AccB7I CCANNNNNTGG 3 cut(s) 1555, 1715, 3386
AccBSI CCGCTC 1 cut(s) 18
AccII CGCG 4 cut(s) 244, 386, 509, 3506
AccIII TCCGGA 1 cut(s) 2699
AclI AACGTT 1 cut(s) 1440
AclWI GGATC 3 cut(s) 1148, 2652, 3253
AcuI CTGAAG 4 cut(s) 11, 2115, 3114, 3135
AcyI GRCGYC 2 cut(s) 114, 867
AfaI GTAC 4 cut(s) 1312, 2575, 3170, 3555
AfiI CCNNNNNNNGG 8 cut(s) 261, 331, 358, 877, 1457, 1555, 1715, 3386
AflII CTTAAG 2 cut(s) 1907, 2866
AjiI CACGTC 1 cut(s) 1243
AjnI CCWGG 3 cut(s) 1721, 2046, 2351
AjuI GAANNNNNNNTTGG 6 cut(s) 788, 820, 2384, 2416, 3521, 3553
Alw21I GWGCWC 3 cut(s) 56, 3044, 3357
Alw26I GTCTC 6 cut(s) 1710, 1770, 1999, 2008, 2412, 2541
AlwI GGATC 3 cut(s) 1148, 2652, 3253
AlwNI CAGNNNCTG 2 cut(s) 305, 454
Ama87I CYCGRG 1 cut(s) 589
Aor13HI TCCGGA 1 cut(s) 2699
AoxI GGCC 5 cut(s) 263, 648, 855, 2349, 3377
Asp700I GAANNNNTTC 6 cut(s) 416, 755, 1779, 2707, 3530, 3575
AspLEI GCGC 6 cut(s) 246, 388, 454, 640, 1120, 3506
AspS9I GGNCC 5 cut(s) 264, 285, 1023, 1582, 2697
AsuC2I CCSGG 3 cut(s) 268, 332, 644
AsuHPI GGTGA 8 cut(s) 1216, 1570, 1937, 2639, 2857, 3093, 3211, 3499
AsuII TTCGAA 1 cut(s) 669
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 4 cut(s) 285, 1023, 1582, 2697
BaeGI GKGCMC 1 cut(s) 3255
BanII GRGCYC 4 cut(s) 56, 210, 3044, 3357
BauI CACGAG 1 cut(s) 2982
BbsI GAAGAC 7 cut(s) 267, 678, 1968, 2733, 3591, 3669, 3681
Bbv12I GWGCWC 3 cut(s) 56, 3044, 3357
BceAI ACGGC 3 cut(s) 127, 278, 409
BcgI CGANNNNNNTGC 2 cut(s) 2963, 2997
BciT130I CCWGG 3 cut(s) 1723, 2048, 2353
BclI TGATCA 1 cut(s) 1594
BcnI CCSGG 3 cut(s) 268, 332, 644
BcoDI GTCTC 6 cut(s) 1710, 1770, 1999, 2008, 2412, 2541
BfmI CTRYAG 4 cut(s) 1085, 2171, 2859, 3294
BfoI RGCGCY 1 cut(s) 455
BfrI CTTAAG 2 cut(s) 1907, 2866
BfuAI ACCTGC 1 cut(s) 1078
BglII AGATCT 3 cut(s) 593, 1507, 2606
Bme1390I CCNGG 6 cut(s) 268, 332, 644, 1723, 2048, 2353
Bme18I GGWCC 4 cut(s) 285, 1023, 1582, 2697
BmeT110I CYCGRG 1 cut(s) 589
BmgBI CACGTC 1 cut(s) 1243
BmgT120I GGNCC 5 cut(s) 264, 285, 1023, 1582, 2697
BmiI GGNNCC 6 cut(s) 14, 207, 266, 828, 1720, 2698
BmrFI CCNGG 6 cut(s) 268, 332, 644, 1723, 2048, 2353
BmrI ACTGGG 2 cut(s) 1466, 3756
BmuI ACTGGG 2 cut(s) 1466, 3756
BpiI GAAGAC 7 cut(s) 267, 678, 1968, 2733, 3591, 3669, 3681
BplI GAGNNNNNCTC 4 cut(s) 470, 502, 2092, 2124
BpmI CTGGAG 7 cut(s) 224, 872, 990, 1737, 1992, 2712, 3225
Bpu10I CCTNAGC 2 cut(s) 50, 2721
Bpu14I TTCGAA 1 cut(s) 669
BpuEI CTTGAG 4 cut(s) 1587, 1745, 2394, 2773
BpuMI CCSGG 3 cut(s) 268, 332, 644
BsaBI GATNNNNATC 1 cut(s) 1827
BsaHI GRCGYC 2 cut(s) 114, 867
BsaI GGTCTC 2 cut(s) 1710, 2412
BsaJI CCNNGG 3 cut(s) 109, 266, 1747
BsaWI WCCGGW 2 cut(s) 440, 2699
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 1990, 2020
Bsc4I CCNNNNNNNGG 8 cut(s) 261, 331, 358, 877, 1457, 1555, 1715, 3386
Bse1I ACTGG 6 cut(s) 963, 1461, 1543, 2550, 3208, 3751
Bse3DI GCAATG 4 cut(s) 530, 816, 1506, 2215
Bse8I GATNNNNATC 1 cut(s) 1827
BseAI TCCGGA 1 cut(s) 2699
BseBI CCWGG 3 cut(s) 1723, 2048, 2353
BseDI CCNNGG 3 cut(s) 109, 266, 1747
BseGI GGATG 7 cut(s) 20, 403, 901, 1299, 2813, 3522, 3732
BseJI GATNNNNATC 1 cut(s) 1827
BseLI CCNNNNNNNGG 8 cut(s) 261, 331, 358, 877, 1457, 1555, 1715, 3386
BseMI GCAATG 4 cut(s) 530, 816, 1506, 2215
BseMII CTCAG 9 cut(s) 41, 581, 836, 1625, 1985, 2216, 2607, 3342, 3653
BseNI ACTGG 6 cut(s) 963, 1461, 1543, 2550, 3208, 3751
BseRI GAGGAG 8 cut(s) 127, 130, 240, 551, 758, 1502, 3017, 3020
BseSI GKGCMC 1 cut(s) 3255
BsgI GTGCAG 3 cut(s) 440, 2486, 2682
Bsh1236I CGCG 4 cut(s) 244, 386, 509, 3506
BshFI GGCC 5 cut(s) 265, 650, 857, 2351, 3379
BsiHKAI GWGCWC 3 cut(s) 56, 3044, 3357
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 6 cut(s) 268, 332, 441, 643, 2700, 3568
BsiWI CGTACG 1 cut(s) 3553
BslFI GGGAC 4 cut(s) 145, 878, 1729, 3561
BslI CCNNNNNNNGG 8 cut(s) 261, 331, 358, 877, 1457, 1555, 1715, 3386
BsmAI GTCTC 6 cut(s) 1710, 1770, 1999, 2008, 2412, 2541
BsmFI GGGAC 4 cut(s) 145, 878, 1729, 3561
BsmI GAATGC 5 cut(s) 416, 1906, 2042, 2165, 2725
BsnI GGCC 5 cut(s) 265, 650, 857, 2351, 3379
Bso31I GGTCTC 2 cut(s) 1710, 2412
BsoBI CYCGRG 1 cut(s) 589
Bsp119I TTCGAA 1 cut(s) 669
Bsp1286I GDGCHC 5 cut(s) 56, 210, 3044, 3255, 3357
Bsp13I TCCGGA 1 cut(s) 2699
BspANI GGCC 5 cut(s) 265, 650, 857, 2351, 3379
BspCNI CTCAG 9 cut(s) 42, 580, 835, 1624, 1984, 2217, 2608, 3343, 3652
BspEI TCCGGA 1 cut(s) 2699
BspFNI CGCG 4 cut(s) 244, 386, 509, 3506
BspHI TCATGA 1 cut(s) 1366
BspLI GGNNCC 6 cut(s) 14, 207, 266, 828, 1720, 2698
BspMAI CTGCAG 3 cut(s) 1089, 2175, 2863
BspMI ACCTGC 1 cut(s) 1078
BspPI GGATC 3 cut(s) 1148, 2652, 3253
BspQI GCTCTTC 1 cut(s) 3032
BspT104I TTCGAA 1 cut(s) 669
BspTI CTTAAG 2 cut(s) 1907, 2866
BspTNI GGTCTC 2 cut(s) 1710, 2412
BsrBI CCGCTC 1 cut(s) 18
BsrDI GCAATG 4 cut(s) 530, 816, 1506, 2215
BsrI ACTGG 6 cut(s) 963, 1461, 1543, 2550, 3208, 3751
BssECI CCNNGG 3 cut(s) 109, 266, 1747
BssNI GRCGYC 2 cut(s) 114, 867
BssSI CACGAG 1 cut(s) 2982
BssT1I CCWWGG 1 cut(s) 1747
Bst2BI CACGAG 1 cut(s) 2982
Bst2UI CCWGG 3 cut(s) 1723, 2048, 2353
Bst4CI ACNGT 3 cut(s) 2002, 2660, 2750
Bst6I CTCTTC 3 cut(s) 2544, 3032, 3627
BstACI GRCGYC 2 cut(s) 114, 867
BstAFI CTTAAG 2 cut(s) 1907, 2866
BstAPI GCANNNNNTGC 4 cut(s) 959, 1129, 2473, 2687
BstBI TTCGAA 1 cut(s) 669
BstC8I GCNNGC 6 cut(s) 521, 1082, 1882, 2091, 2474, 3565
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 7 cut(s) 20, 403, 901, 1299, 2813, 3522, 3732
BstFNI CGCG 4 cut(s) 244, 386, 509, 3506
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 6 cut(s) 246, 388, 454, 640, 1120, 3506
BstMAI GTCTC 6 cut(s) 1710, 1770, 1999, 2008, 2412, 2541
BstNI CCWGG 3 cut(s) 1723, 2048, 2353
BstNSI RCATGY 5 cut(s) 962, 1677, 2476, 2639, 3567
BstSCI CCNGG 6 cut(s) 266, 330, 642, 1721, 2046, 2351
BstSFI CTRYAG 4 cut(s) 1085, 2171, 2859, 3294
BstSLI GKGCMC 1 cut(s) 3255
BstUI CGCG 4 cut(s) 244, 386, 509, 3506
BstV2I GAAGAC 7 cut(s) 267, 678, 1968, 2733, 3591, 3669, 3681
BstX2I RGATCY 5 cut(s) 593, 1153, 1507, 2606, 3245
BstYI RGATCY 5 cut(s) 593, 1153, 1507, 2606, 3245
BsuRI GGCC 5 cut(s) 265, 650, 857, 2351, 3379
BtgI CCRYGG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 1243
BtsCI GGATG 7 cut(s) 20, 403, 901, 1299, 2813, 3522, 3732
BtsI GCAGTG 2 cut(s) 1026, 2474
BtsIMutI CAGTG 6 cut(s) 557, 1026, 1385, 2340, 2474, 3111
BveI ACCTGC 1 cut(s) 1078
Cac8I GCNNGC 6 cut(s) 521, 1082, 1882, 2091, 2474, 3565
CaiI CAGNNNCTG 2 cut(s) 305, 454
CciI TCATGA 1 cut(s) 1366
CfoI GCGC 6 cut(s) 246, 388, 454, 640, 1120, 3506
Cfr13I GGNCC 5 cut(s) 264, 285, 1023, 1582, 2697
CseI GACGC 2 cut(s) 103, 3108
CsiI ACCWGGT 1 cut(s) 2046
Csp6I GTAC 4 cut(s) 1311, 2574, 3169, 3554
CviQI GTAC 4 cut(s) 1311, 2574, 3169, 3554
Eam1104I CTCTTC 3 cut(s) 2544, 3032, 3627
EarI CTCTTC 3 cut(s) 2544, 3032, 3627
EciI GGCGGA 3 cut(s) 25, 46, 908
Ecl136II GAGCTC 3 cut(s) 54, 3042, 3355
Eco130I CCWWGG 1 cut(s) 1747
Eco147I AGGCCT 2 cut(s) 650, 857
Eco24I GRGCYC 4 cut(s) 56, 210, 3044, 3357
Eco31I GGTCTC 2 cut(s) 1710, 2412
Eco47I GGWCC 4 cut(s) 285, 1023, 1582, 2697
Eco53kI GAGCTC 3 cut(s) 54, 3042, 3355
Eco57I CTGAAG 4 cut(s) 11, 2115, 3114, 3135
Eco88I CYCGRG 1 cut(s) 589
EcoICRI GAGCTC 3 cut(s) 54, 3042, 3355
EcoRI GAATTC 3 cut(s) 44, 1096, 2392
EcoRII CCWGG 3 cut(s) 1721, 2046, 2351
EcoT14I CCWWGG 1 cut(s) 1747
EcoT22I ATGCAT 3 cut(s) 2042, 2478, 2641
EcoT38I GRGCYC 4 cut(s) 56, 210, 3044, 3357
ErhI CCWWGG 1 cut(s) 1747
FalI AAGNNNNNCTT 8 cut(s) 1187, 1219, 1830, 1862, 1901, 1933, 3029, 3061
FaqI GGGAC 4 cut(s) 145, 878, 1729, 3561
FauI CCCGC 3 cut(s) 249, 502, 2291
FauNDI CATATG 3 cut(s) 2532, 3052, 3735
FbaI TGATCA 1 cut(s) 1594
FokI GGATG 7 cut(s) 7, 390, 908, 1286, 2820, 3529, 3739
FriOI GRGCYC 4 cut(s) 56, 210, 3044, 3357
GlaI GCGC 6 cut(s) 245, 387, 453, 639, 1119, 3505
GsuI CTGGAG 7 cut(s) 224, 872, 990, 1737, 1992, 2712, 3225
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 5 cut(s) 265, 650, 857, 2351, 3379
HapII CCGG 6 cut(s) 268, 332, 441, 643, 2700, 3568
HgaI GACGC 2 cut(s) 103, 3108
HhaI GCGC 6 cut(s) 246, 388, 454, 640, 1120, 3506
Hin1I GRCGYC 2 cut(s) 114, 867
Hin6I GCGC 6 cut(s) 244, 386, 452, 638, 1118, 3504
HinP1I GCGC 6 cut(s) 244, 386, 452, 638, 1118, 3504
HincII GTYRAC 2 cut(s) 438, 2656
HindII GTYRAC 2 cut(s) 438, 2656
HindIII AAGCTT 2 cut(s) 1043, 3096
HpaI GTTAAC 1 cut(s) 2656
HpaII CCGG 6 cut(s) 268, 332, 441, 643, 2700, 3568
HphI GGTGA 8 cut(s) 1216, 1570, 1937, 2639, 2857, 3093, 3211, 3499
Hpy166II GTNNAC 5 cut(s) 438, 625, 1844, 2656, 2934
Hpy8I GTNNAC 5 cut(s) 438, 625, 1844, 2656, 2934
Hpy99I CGWCG 2 cut(s) 44, 2754
HpyCH4III ACNGT 3 cut(s) 2002, 2660, 2750
HpyCH4IV ACGT 3 cut(s) 867, 1242, 1440
HpySE526I ACGT 3 cut(s) 867, 1242, 1440
Hsp92I GRCGYC 2 cut(s) 114, 867
HspAI GCGC 6 cut(s) 244, 386, 452, 638, 1118, 3504
Kpn2I TCCGGA 1 cut(s) 2699
Ksp22I TGATCA 1 cut(s) 1594
KspAI GTTAAC 1 cut(s) 2656
LguI GCTCTTC 1 cut(s) 3032
MabI ACCWGGT 1 cut(s) 2046
MaeII ACGT 3 cut(s) 867, 1242, 1440
MaeIII GTNAC 6 cut(s) 2050, 2942, 3063, 3088, 3199, 3442
MbiI CCGCTC 1 cut(s) 18
MfeI CAATTG 1 cut(s) 389
MflI RGATCY 5 cut(s) 593, 1153, 1507, 2606, 3245
MhlI GDGCHC 5 cut(s) 56, 210, 3044, 3255, 3357
MlyI GAGTC 9 cut(s) 479, 488, 556, 595, 639, 1895, 2429, 3306, 3543
MmeI TCCRAC 7 cut(s) 153, 408, 462, 1560, 2610, 2769, 2949
Mph1103I ATGCAT 3 cut(s) 2042, 2478, 2641
MroI TCCGGA 1 cut(s) 2699
MroXI GAANNNNTTC 6 cut(s) 416, 755, 1779, 2707, 3530, 3575
MslI CAYNNNNRTG 2 cut(s) 2781, 3738
MspA1I CMGCKG 4 cut(s) 727, 790, 1057, 3299
MspCI CTTAAG 2 cut(s) 1907, 2866
MspI CCGG 6 cut(s) 268, 332, 441, 643, 2700, 3568
MspR9I CCNGG 6 cut(s) 268, 332, 644, 1723, 2048, 2353
MunI CAATTG 1 cut(s) 389
Mva1269I GAATGC 5 cut(s) 416, 1906, 2042, 2165, 2725
MvaI CCWGG 3 cut(s) 1723, 2048, 2353
MvnI CGCG 4 cut(s) 244, 386, 509, 3506
NciI CCSGG 3 cut(s) 268, 332, 644
NdeI CATATG 3 cut(s) 2532, 3052, 3735
NlaIV GGNNCC 6 cut(s) 14, 207, 266, 828, 1720, 2698
NmeAIII GCCGAG 1 cut(s) 570
NmuCI GTSAC 2 cut(s) 2050, 3199
NsiI ATGCAT 3 cut(s) 2042, 2478, 2641
NspI RCATGY 5 cut(s) 962, 1677, 2476, 2639, 3567
NspV TTCGAA 1 cut(s) 669
PaeI GCATGC 2 cut(s) 2476, 3567
PaeR7I CTCGAG 1 cut(s) 589
PagI TCATGA 1 cut(s) 1366
PceI AGGCCT 2 cut(s) 650, 857
PciSI GCTCTTC 1 cut(s) 3032
PctI GAATGC 5 cut(s) 416, 1906, 2042, 2165, 2725
PdmI GAANNNNTTC 6 cut(s) 416, 755, 1779, 2707, 3530, 3575
Pfl23II CGTACG 1 cut(s) 3553
PflFI GACNNNGTC 2 cut(s) 1458, 1478
PflMI CCANNNNNTGG 3 cut(s) 1555, 1715, 3386
PfoI TCCNGGA 1 cut(s) 642
PleI GAGTC 9 cut(s) 479, 487, 555, 595, 638, 1894, 2428, 3306, 3543
PpsI GAGTC 9 cut(s) 479, 487, 555, 595, 638, 1894, 2428, 3306, 3543
PsiI TTATAA 2 cut(s) 1013, 1523
Psp124BI GAGCTC 3 cut(s) 56, 3044, 3357
Psp1406I AACGTT 1 cut(s) 1440
Psp6I CCWGG 3 cut(s) 1721, 2046, 2351
PspGI CCWGG 3 cut(s) 1721, 2046, 2351
PspLI CGTACG 1 cut(s) 3553
PspN4I GGNNCC 6 cut(s) 14, 207, 266, 828, 1720, 2698
PspPI GGNCC 5 cut(s) 264, 285, 1023, 1582, 2697
PstI CTGCAG 3 cut(s) 1089, 2175, 2863
PstNI CAGNNNCTG 2 cut(s) 305, 454
PsuI RGATCY 5 cut(s) 593, 1153, 1507, 2606, 3245
PsyI GACNNNGTC 2 cut(s) 1458, 1478
PvuII CAGCTG 3 cut(s) 790, 1057, 3299
RsaI GTAC 4 cut(s) 1312, 2575, 3170, 3555
RsaNI GTAC 4 cut(s) 1311, 2574, 3169, 3554
RseI CAYNNNNRTG 2 cut(s) 2781, 3738
SacI GAGCTC 3 cut(s) 56, 3044, 3357
SapI GCTCTTC 1 cut(s) 3032
Sau96I GGNCC 5 cut(s) 264, 285, 1023, 1582, 2697
SchI GAGTC 9 cut(s) 479, 488, 556, 595, 639, 1895, 2429, 3306, 3543
ScrFI CCNGG 6 cut(s) 268, 332, 644, 1723, 2048, 2353
SduI GDGCHC 5 cut(s) 56, 210, 3044, 3255, 3357
SexAI ACCWGGT 1 cut(s) 2046
SfcI CTRYAG 4 cut(s) 1085, 2171, 2859, 3294
Sfr274I CTCGAG 1 cut(s) 589
SfuI TTCGAA 1 cut(s) 669
SinI GGWCC 4 cut(s) 285, 1023, 1582, 2697
SlaI CTCGAG 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 2781, 3738
SmlI CTYRAG 7 cut(s) 589, 1602, 1760, 1907, 2409, 2788, 2866
SmoI CTYRAG 7 cut(s) 589, 1602, 1760, 1907, 2409, 2788, 2866
SphI GCATGC 2 cut(s) 2476, 3567
SseBI AGGCCT 2 cut(s) 650, 857
SstI GAGCTC 3 cut(s) 56, 3044, 3357
StuI AGGCCT 2 cut(s) 650, 857
StyD4I CCNGG 6 cut(s) 266, 330, 642, 1721, 2046, 2351
StyI CCWWGG 1 cut(s) 1747
TaaI ACNGT 3 cut(s) 2002, 2660, 2750
TaiI ACGT 3 cut(s) 870, 1245, 1443
TaqII GACCGA 1 cut(s) 1953
TatI WGTACW 2 cut(s) 1310, 3168
TauI GCSGC 4 cut(s) 13, 62, 727, 2888
TscAI CASTG 6 cut(s) 564, 1033, 1392, 2340, 2474, 3118
TseFI GTSAC 2 cut(s) 2050, 3199
Tsp45I GTSAC 2 cut(s) 2050, 3199
TspDTI ATGAA 6 cut(s) 1271, 1839, 2057, 2226, 2894, 3756
TspGWI ACGGA 2 cut(s) 819, 1651
TspRI CASTG 6 cut(s) 564, 1033, 1392, 2340, 2474, 3118
Tth111I GACNNNGTC 2 cut(s) 1458, 1478
Van91I CCANNNNNTGG 3 cut(s) 1555, 1715, 3386
Vha464I CTTAAG 2 cut(s) 1907, 2866
VpaK11BI GGWCC 4 cut(s) 285, 1023, 1582, 2697
XbaI TCTAGA 2 cut(s) 1819, 2416
XceI RCATGY 5 cut(s) 962, 1677, 2476, 2639, 3567
XcmI CCANNNNNNNNNTGG 1 cut(s) 2201
XhoI CTCGAG 1 cut(s) 589
XmnI GAANNNNTTC 6 cut(s) 416, 755, 1779, 2707, 3530, 3575
ZraI GACGTC 1 cut(s) 868
Zsp2I ATGCAT 3 cut(s) 2042, 2478, 2641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.