Rw2G032600

Condensin II non structural maintenance of chromosomes subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006247
Physical Location & Seq
Forward (+)
53670460 .. 53676154
5695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032600.1

Sequence Viewer

Length: 2799 bp
ATGGAGAAGCGGCTCCGCTCCTCTCTCCAATCCTCCGCCGAAGAGTTTCTGACCTCCGCCACCAAGCTCAGCCTCAAATCCTCAAAGCCCTCTCTCAAAACCCTAATCCACAAAATCAAACCCTCCTCCGACCTCTCCTCCTCTCTCCCCATCTCTCTCTCCCACTCCATTTCCGACGCCATCCTCTCCTTCCAGACCCTTCTTCTTCAACCCAACCCCAACCCCAACCCTAGATCCCCTCGCTCTCCCCCCACCAAGCGCCCCCGCAGGTCCAGCCGGAGCTCCAAGACCCGACCCGACCGCGAACCCGAACCAGACCTCCATCCGGACCCGAATCCTGAAGAGGAGAAGCATCAACTGCTCTCGAAGCTTCAGATTCTCGCCTGTATCGCGCAACTATGCGCCGGGCATCCGGCGAAGGCGTTCCAGCCGTTGGATTTGTTGCCGGGAGTTCAAGCGCTTCACGACAGTCTGATAATTCTGGAGTCGGACTCGGCGCTGGTATCGGCGATTGCGAGCTTGTGCGAGCAGTGGTGGAAGGAGGAGTTGCCGGGCCGAGAATCGCTGATATCTCAGTCGCTCCCTTTCTTGCTCTCGAGATCTCTCACTCTGAACAAGAAGGCAGACGTCCACAGAGTCTACGCCCTCCGCGAGGCTTTCACCTGCTTCGATTTCGAGGACGAGAGCATTGAGGACTTGAAGCTGTTGCTAATCCGGTGCGTGATTTCGCCGCTGTTTCTGAAAACAGAGGAGGGGAGGAAGTTTCTGGCGTTTCTGTTTGGGTTGAGTTCGCAACTGTTGAAGGAGGTTTTGGCTGTAATCAAGTCTCAGATTCCGTTTGGAAGGAAGTCGATGCTGGAGGCCTACGGTGACGTTTTGTTCCGGGCTTGGAAAGTGGCAGAGGGGGAGCTGAGGAGTGAGATTGAGAACGGGTTCTTGCAGGTTTTGATTGAGGGTGCTATACACGCCAGCTCTGGAGGATTTGCTGCTTCTATTAGAAGGGTTTTGGGAGGGTTTATAAACCAGAGGACCACTGCTGGTGTCGAGAAGCTTCTTTTCCAGCTCTCGGAGCCTGTCATATTTCGCTCTTTGCAGGTTGCAAATTCTAATGTTCGTCAAAATGCGCTGCATTTACTCTTGGACTTGTTCCCTCTTGAAGATCCTGATTCGACTAAAGAGGTTAAAGATTCATTGCTTGATAAGCAGTTCTTTTTATTGGGGAAATTACTTGTAGATGATTGTCCAGAGGTGAGAGTGGTTGCAGTGGAAGGTTCTTGTCGTATTCTTCATCTGTTTTGGGAAGTAATCCCTTCGCCTACCATCACAAAGTTAGTTACTAGAATTTTTGATGATATGTCACGTGATACAAGTCATGAGGTTAGGCTTTCCACACTGAATGGCACTATGTATTTGTTTGGCAATCCTCTCTGTCATCAATTTCTCAATGTGCTTCTACCCAGATTGGGGCATTTGATGATGGACAATGTCCTATCAATAAGAGTTGCCATGGTAGAACTCCTCCTTCTCATAAGGGATATTCAAAACTTCCATTTCCATAAGGTAGTGAAGTTAGATGTACTATTATCTACCCTTGAAAATGATCAATCTCAAGTTGCTCAGAAAATTACAAGGTTGCTTATGCCATCATACTTTCCCTCAAAAGAAACTGTTGAAACAGCATGCAATCGTTGTACTACACTTGTAAAAAGGTCTCCGATGGCTGGAGCAAGGTTTTGTGAATTTGCTGCGTCCCAAGGGGCATCTCTTAAGTCCCTGATGGAACTTGTTAGGGTAATGATTACTTTGGTCCTTCCACCTAATAATCTGGATGAACAACAAGTTGAGGGTTTACTTACTTCTTCTTCCAACCTTTGCAATACCCTAGCTGGTGATCCAGTTTTCAAGAAGGAGCTTAAAGAATTCTTTGATGGTGAAAAGTTGAAGTACTTGATTGCTGCCGCACCTACCGGGCCTGCTCAGTCTTGTGTATTCAACATTGTTTCAACTGTCTCTCCTGATGATGTGGCTGGACTTCTTGATGAATGTATGCATCTGGTGACTAATTGTAGTGGTTTATCCAAAAATGTGGAGAAGCAATCTGAAGTGAGGTCTGCTCACAATTTGTTGCTGTCTTGTGATGCGATTGATGGTATGCTGGAAGCTCTAACAGCACTTCTGCAGAAATCAGCCTATCGTTGCCATATTAAATATGGCAGTGAAATTAAGAACTTAAGTGTTTTCTCTGCGAAAAGAAAGAAATCAAAGTCCTCTGGCAAAATTTCAGCTCGACAGAAACATGTTAGTGGAAAAAAAGTCACCAGTTTTGAGGACGATTATTCAGTCGCGGCAGGAATAGCCTGGCAAATCAGGGACTTGCTTGTATCTGTCAATACTCGGAAGGCTATATTGGGATCTCAAATTCTAGAATCTTTACTAATATGTCTAAAGGTCATTTCTGAGGTCAGCATTGTGCAGTGCATGGATTATGAATATGTGGACACATTTCCTGTTTCAGCATATACAGCTCTTGCGCTGCATTTGTCGCTTCAAAATGTCTCCTCCAATAAGAATGACGGGACGGATTCATCAGGGTCTACTTTGGAGCAGACCGAGTTGGAACAGGCATTGGATCATGTGCTTAATTGTACAGAGAAGCTATGTGGAATAGGCGATTCTGGACAGTCTAGCAGAGTTTATTCAGAATCTAAGCATGCTAGTGATAAGTCTGCGAGTCGTCGTGAAAAAAGGCACAGAGAACCTCAAACACATGCCTCCATTGCAAGTGATAGTGGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Condensin2nSMC

Protein Analysis

932

Amino Acids

103.26

Weight (kDa)

7.53

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Condensin2nSMC PF12422 239 - 387 2.1e-56 Condensin II non structural maintenance of chromosomes subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1019
AarI CACCTGC 1 cut(s) 671
AatII GACGTC 1 cut(s) 630
Acc36I ACCTGC 4 cut(s) 258, 671, 931, 1084
AccB7I CCANNNNNTGG 1 cut(s) 433
AccBSI CCGCTC 1 cut(s) 18
AccI GTMKAC 2 cut(s) 639, 2594
AccII CGCG 4 cut(s) 303, 392, 651, 2345
AccIII TCCGGA 1 cut(s) 325
AclWI GGATC 5 cut(s) 228, 1154, 1886, 2419, 2637
AcsI RAATTY 6 cut(s) 1102, 1341, 1739, 1919, 2277, 2418
AcuI CTGAAG 3 cut(s) 356, 360, 2121
AcvI CACGTG 1 cut(s) 1361
AcyI GRCGYC 2 cut(s) 177, 627
AfaI GTAC 4 cut(s) 1578, 1693, 1946, 2647
AfeI AGCGCT 1 cut(s) 459
AfiI CCNNNNNNNGG 7 cut(s) 325, 433, 652, 1066, 1463, 1464, 1721
AflII CTTAAG 2 cut(s) 1766, 2230
AflIII ACRYGT 1 cut(s) 2296
AjnI CCWGG 1 cut(s) 2357
AjuI GAANNNNNNNTTGG 4 cut(s) 794, 826, 2390, 2422
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 4 cut(s) 831, 1716, 2014, 2560
AlwI GGATC 5 cut(s) 228, 1154, 1886, 2419, 2637
Ama87I CYCGRG 1 cut(s) 595
Aor13HI TCCGGA 1 cut(s) 325
Aor51HI AGCGCT 1 cut(s) 459
AoxI GGCC 3 cut(s) 553, 861, 1970
ApeKI GCWGC 5 cut(s) 986, 1126, 1745, 1955, 2533
ApoI RAATTY 6 cut(s) 1102, 1341, 1739, 1919, 2277, 2418
Asp700I GAANNNNTTC 3 cut(s) 45, 422, 932
AspLEI GCGC 7 cut(s) 261, 394, 404, 460, 499, 1126, 2533
AspS9I GGNCC 6 cut(s) 270, 328, 553, 1029, 1807, 1970
AsuC2I CCSGG 5 cut(s) 406, 447, 552, 884, 1969
AsuHPI GGTGA 7 cut(s) 652, 881, 1261, 1901, 1943, 2068, 2308
AvaI CYCGRG 1 cut(s) 595
AvaII GGWCC 4 cut(s) 270, 328, 1029, 1807
BanII GRGCYC 1 cut(s) 284
BbrPI CACGTG 1 cut(s) 1361
Bbv12I GWGCWC 1 cut(s) 284
BbvCI CCTCAGC 1 cut(s) 911
BbvI GCAGC 5 cut(s) 973, 1113, 1732, 1942, 2520
BceAI ACGGC 1 cut(s) 415
BciT130I CCWGG 1 cut(s) 2359
BclI TGATCA 1 cut(s) 1600
BcnI CCSGG 5 cut(s) 406, 447, 552, 884, 1969
BcoDI GTCTC 4 cut(s) 831, 1716, 2014, 2560
BfaI CTAG 6 cut(s) 231, 1338, 1883, 2423, 2685, 2715
BfmI CTRYAG 1 cut(s) 2177
BfoI RGCGCY 3 cut(s) 262, 461, 500
BfrI CTTAAG 2 cut(s) 1766, 2230
BfuAI ACCTGC 4 cut(s) 258, 671, 931, 1084
BglII AGATCT 1 cut(s) 599
BisI GCNGC 9 cut(s) 11, 731, 987, 1127, 1746, 1956, 1959, 2346, 2534
BlpI GCTNAGC 1 cut(s) 68
BlsI GCNGC 9 cut(s) 12, 732, 988, 1128, 1747, 1957, 1960, 2347, 2535
BmcAI AGTACT 1 cut(s) 1946
Bme1390I CCNGG 6 cut(s) 406, 447, 552, 884, 1969, 2359
Bme18I GGWCC 4 cut(s) 270, 328, 1029, 1807
BmeT110I CYCGRG 1 cut(s) 595
BmgT120I GGNCC 6 cut(s) 270, 328, 553, 1029, 1807, 1970
BmiI GGNNCC 3 cut(s) 14, 330, 1071
BmrFI CCNGG 6 cut(s) 406, 447, 552, 884, 1969, 2359
BmsI GCATC 6 cut(s) 361, 418, 843, 1769, 2059, 2128
BplI GAGNNNNNCTC 4 cut(s) 476, 508, 2098, 2130
BpmI CTGGAG 4 cut(s) 503, 878, 996, 1743
Bpu10I CCTNAGC 1 cut(s) 911
Bpu1102I GCTNAGC 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 1593
BpuMI CCSGG 5 cut(s) 406, 447, 552, 884, 1969
BsaAI YACGTR 1 cut(s) 1361
BsaHI GRCGYC 2 cut(s) 177, 627
BsaI GGTCTC 1 cut(s) 1716
BsaJI CCNNGG 2 cut(s) 1506, 1753
BsaWI WCCGGW 2 cut(s) 325, 714
BsaXI ACNNNNNCTCC 6 cut(s) 122, 152, 303, 333, 1996, 2026
Bsc4I CCNNNNNNNGG 7 cut(s) 325, 433, 652, 1066, 1463, 1464, 1721
Bse1I ACTGG 2 cut(s) 1895, 2319
Bse3DI GCAATG 2 cut(s) 1190, 2775
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 1 cut(s) 2359
BseDI CCNNGG 2 cut(s) 1506, 1753
BseGI GGATG 4 cut(s) 180, 322, 409, 1834
BseLI CCNNNNNNNGG 7 cut(s) 325, 433, 652, 1066, 1463, 1464, 1721
BseMI GCAATG 2 cut(s) 1190, 2775
BseMII CTCAG 7 cut(s) 82, 587, 842, 902, 1631, 1991, 2448
BseNI ACTGG 2 cut(s) 1895, 2319
BseXI GCAGC 5 cut(s) 973, 1113, 1732, 1942, 2520
BsgI GTGCAG 1 cut(s) 2492
Bsh1236I CGCG 4 cut(s) 303, 392, 651, 2345
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 3 cut(s) 555, 863, 1972
BsiEI CGRYCG 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 595
BsiSI CCGG 9 cut(s) 277, 326, 405, 413, 446, 551, 715, 883, 1968
BslFI GGGAC 4 cut(s) 1735, 1756, 2384, 2590
BslI CCNNNNNNNGG 7 cut(s) 325, 433, 652, 1066, 1463, 1464, 1721
BsmAI GTCTC 4 cut(s) 831, 1716, 2014, 2560
BsmFI GGGAC 4 cut(s) 1735, 1756, 2384, 2590
BsnI GGCC 3 cut(s) 555, 863, 1972
Bso31I GGTCTC 1 cut(s) 1716
BsoBI CYCGRG 1 cut(s) 595
Bsp1286I GDGCHC 1 cut(s) 284
Bsp13I TCCGGA 1 cut(s) 325
Bsp1407I TGTACA 1 cut(s) 2645
Bsp143I GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
Bsp1720I GCTNAGC 1 cut(s) 68
Bsp19I CCATGG 1 cut(s) 1506
BspANI GGCC 3 cut(s) 555, 863, 1972
BspCNI CTCAG 7 cut(s) 81, 586, 841, 903, 1630, 1990, 2449
BspEI TCCGGA 1 cut(s) 325
BspFNI CGCG 4 cut(s) 303, 392, 651, 2345
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 3 cut(s) 14, 330, 1071
BspMAI CTGCAG 1 cut(s) 2181
BspMI ACCTGC 4 cut(s) 258, 671, 931, 1084
BspPI GGATC 5 cut(s) 228, 1154, 1886, 2419, 2637
BspTI CTTAAG 2 cut(s) 1766, 2230
BspTNI GGTCTC 1 cut(s) 1716
BsrBI CCGCTC 1 cut(s) 18
BsrDI GCAATG 2 cut(s) 1190, 2775
BsrGI TGTACA 1 cut(s) 2645
BsrI ACTGG 2 cut(s) 1895, 2319
BssECI CCNNGG 2 cut(s) 1506, 1753
BssMI GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
BssNI GRCGYC 2 cut(s) 177, 627
BssT1I CCWWGG 2 cut(s) 1506, 1753
Bst2UI CCWGG 1 cut(s) 2359
Bst4CI ACNGT 6 cut(s) 470, 798, 869, 1669, 2008, 2682
Bst6I CTCTTC 2 cut(s) 36, 336
BstACI GRCGYC 2 cut(s) 177, 627
BstAFI CTTAAG 2 cut(s) 1766, 2230
BstAPI GCANNNNNTGC 1 cut(s) 358
BstAUI TGTACA 1 cut(s) 2645
BstBAI YACGTR 1 cut(s) 1361
BstC8I GCNNGC 6 cut(s) 517, 527, 970, 1681, 1974, 2712
BstDEI CTNAG 8 cut(s) 68, 573, 828, 911, 1617, 1977, 2457, 2706
BstDSI CCRYGG 1 cut(s) 1506
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 4 cut(s) 180, 322, 409, 1834
BstFNI CGCG 4 cut(s) 303, 392, 651, 2345
BstH2I RGCGCY 3 cut(s) 262, 461, 500
BstHHI GCGC 7 cut(s) 261, 394, 404, 460, 499, 1126, 2533
BstKTI GATC 7 cut(s) 236, 602, 1162, 1603, 1894, 2414, 2632
BstMAI GTCTC 4 cut(s) 831, 1716, 2014, 2560
BstMBI GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
BstMCI CGRYCG 1 cut(s) 301
BstNI CCWGG 1 cut(s) 2359
BstNSI RCATGY 4 cut(s) 1683, 2300, 2714, 2771
BstSCI CCNGG 6 cut(s) 404, 445, 550, 882, 1967, 2357
BstSFI CTRYAG 1 cut(s) 2177
BstUI CGCG 4 cut(s) 303, 392, 651, 2345
BstV1I GCAGC 5 cut(s) 973, 1113, 1732, 1942, 2520
BstX2I RGATCY 4 cut(s) 233, 599, 1159, 2411
BstXI CCANNNNNNTGG 1 cut(s) 2086
BstYI RGATCY 4 cut(s) 233, 599, 1159, 2411
BsuRI GGCC 3 cut(s) 555, 863, 1972
BtgI CCRYGG 1 cut(s) 1506
BtsCI GGATG 4 cut(s) 180, 322, 409, 1834
BtsI GCAGTG 5 cut(s) 536, 1032, 1269, 2221, 2480
BtsIMutI CAGTG 6 cut(s) 536, 1032, 1269, 1391, 2221, 2480
BveI ACCTGC 4 cut(s) 258, 671, 931, 1084
Cac8I GCNNGC 6 cut(s) 517, 527, 970, 1681, 1974, 2712
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 7 cut(s) 261, 394, 404, 460, 499, 1126, 2533
Cfr13I GGNCC 6 cut(s) 270, 328, 553, 1029, 1807, 1970
CseI GACGC 2 cut(s) 185, 1737
Csp6I GTAC 4 cut(s) 1577, 1692, 1945, 2646
CviAII CATG 8 cut(s) 1373, 1507, 1680, 2297, 2479, 2633, 2711, 2768
CviQI GTAC 4 cut(s) 1577, 1692, 1945, 2646
DdeI CTNAG 8 cut(s) 68, 573, 828, 911, 1617, 1977, 2457, 2706
DpnI GATC 7 cut(s) 235, 601, 1161, 1602, 1893, 2413, 2631
DpnII GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
Eam1104I CTCTTC 2 cut(s) 36, 336
EarI CTCTTC 2 cut(s) 36, 336
EciI GGCGGA 2 cut(s) 25, 46
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 1506, 1753
Eco147I AGGCCT 1 cut(s) 863
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 1716
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 4 cut(s) 270, 328, 1029, 1807
Eco47III AGCGCT 1 cut(s) 459
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 3 cut(s) 356, 360, 2121
Eco72I CACGTG 1 cut(s) 1361
Eco88I CYCGRG 1 cut(s) 595
EcoICRI GAGCTC 1 cut(s) 282
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 1919
EcoRII CCWGG 1 cut(s) 2357
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 2 cut(s) 1506, 1753
EcoT22I ATGCAT 1 cut(s) 2052
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 1506, 1753
FaeI CATG 8 cut(s) 1376, 1510, 1683, 2300, 2482, 2636, 2714, 2771
FalI AAGNNNNNCTT 2 cut(s) 1193, 1225
FaqI GGGAC 4 cut(s) 1735, 1756, 2384, 2590
FatI CATG 8 cut(s) 1372, 1506, 1679, 2296, 2478, 2632, 2710, 2767
FauI CCCGC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 1600
FblI GTMKAC 2 cut(s) 639, 2594
Fnu4HI GCNGC 9 cut(s) 11, 731, 987, 1127, 1746, 1956, 1959, 2346, 2534
FokI GGATG 4 cut(s) 167, 309, 396, 1841
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 9 cut(s) 11, 731, 987, 1127, 1746, 1956, 1959, 2346, 2534
FspBI CTAG 6 cut(s) 231, 1338, 1883, 2423, 2685, 2715
GlaI GCGC 7 cut(s) 260, 393, 403, 459, 498, 1125, 2532
GluI GCNGC 9 cut(s) 11, 731, 987, 1127, 1746, 1956, 1959, 2346, 2534
GsuI CTGGAG 4 cut(s) 503, 878, 996, 1743
HaeII RGCGCY 3 cut(s) 262, 461, 500
HaeIII GGCC 3 cut(s) 555, 863, 1972
HapII CCGG 9 cut(s) 277, 326, 405, 413, 446, 551, 715, 883, 1968
HgaI GACGC 2 cut(s) 185, 1737
HhaI GCGC 7 cut(s) 261, 394, 404, 460, 499, 1126, 2533
Hin1I GRCGYC 2 cut(s) 177, 627
Hin1II CATG 8 cut(s) 1376, 1510, 1683, 2300, 2482, 2636, 2714, 2771
Hin6I GCGC 7 cut(s) 259, 392, 402, 458, 497, 1124, 2531
HinP1I GCGC 7 cut(s) 259, 392, 402, 458, 497, 1124, 2531
HindIII AAGCTT 2 cut(s) 368, 1049
HpaII CCGG 9 cut(s) 277, 326, 405, 413, 446, 551, 715, 883, 1968
HphI GGTGA 7 cut(s) 652, 881, 1261, 1901, 1943, 2068, 2308
Hpy166II GTNNAC 5 cut(s) 631, 640, 1850, 2497, 2595
Hpy8I GTNNAC 5 cut(s) 631, 640, 1850, 2497, 2595
Hpy99I CGWCG 2 cut(s) 179, 2739
HpyCH4III ACNGT 6 cut(s) 470, 798, 869, 1669, 2008, 2682
HpyCH4IV ACGT 3 cut(s) 627, 873, 1360
HpyF3I CTNAG 8 cut(s) 68, 573, 828, 911, 1617, 1977, 2457, 2706
HpySE526I ACGT 3 cut(s) 627, 873, 1360
Hsp92I GRCGYC 2 cut(s) 177, 627
Hsp92II CATG 8 cut(s) 1376, 1510, 1683, 2300, 2482, 2636, 2714, 2771
HspAI GCGC 7 cut(s) 259, 392, 402, 458, 497, 1124, 2531
Kpn2I TCCGGA 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 1600
Kzo9I GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
Lsp1109I GCAGC 5 cut(s) 973, 1113, 1732, 1942, 2520
LweI GCATC 6 cut(s) 361, 418, 843, 1769, 2059, 2128
MaeI CTAG 6 cut(s) 231, 1338, 1883, 2423, 2685, 2715
MaeII ACGT 3 cut(s) 627, 873, 1360
MaeIII GTNAC 5 cut(s) 869, 1333, 1356, 2056, 2314
MalI GATC 7 cut(s) 235, 601, 1161, 1602, 1893, 2413, 2631
MbiI CCGCTC 1 cut(s) 18
MboI GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
MboII GAAGA 8 cut(s) 53, 194, 197, 353, 1169, 1277, 1851, 1854
MflI RGATCY 4 cut(s) 233, 599, 1159, 2411
MhlI GDGCHC 1 cut(s) 284
MlyI GAGTC 4 cut(s) 485, 494, 645, 2740
MmeI TCCRAC 6 cut(s) 153, 198, 414, 468, 1890, 2595
Mph1103I ATGCAT 1 cut(s) 2052
MroI TCCGGA 1 cut(s) 325
MroXI GAANNNNTTC 3 cut(s) 45, 422, 932
MseI TTAA 7 cut(s) 1182, 1767, 1914, 2205, 2223, 2231, 2640
MslI CAYNNNNRTG 2 cut(s) 2301, 2715
MspA1I CMGCKG 1 cut(s) 733
MspCI CTTAAG 2 cut(s) 1766, 2230
MspI CCGG 9 cut(s) 277, 326, 405, 413, 446, 551, 715, 883, 1968
MspR9I CCNGG 6 cut(s) 406, 447, 552, 884, 1969, 2359
MvaI CCWGG 1 cut(s) 2359
MvnI CGCG 4 cut(s) 303, 392, 651, 2345
NciI CCSGG 5 cut(s) 406, 447, 552, 884, 1969
NcoI CCATGG 1 cut(s) 1506
NdeII GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
NlaIII CATG 8 cut(s) 1376, 1510, 1683, 2300, 2482, 2636, 2714, 2771
NlaIV GGNNCC 3 cut(s) 14, 330, 1071
NmeAIII GCCGAG 2 cut(s) 473, 581
NmuCI GTSAC 4 cut(s) 869, 1356, 2056, 2314
NsiI ATGCAT 1 cut(s) 2052
NspI RCATGY 4 cut(s) 1683, 2300, 2714, 2771
PaeI GCATGC 2 cut(s) 1683, 2714
PaeR7I CTCGAG 1 cut(s) 595
PagI TCATGA 1 cut(s) 1372
PaqCI CACCTGC 1 cut(s) 671
PceI AGGCCT 1 cut(s) 863
PciI ACATGT 1 cut(s) 2296
PcsI WCGNNNNNNNCGW 2 cut(s) 512, 648
PdmI GAANNNNTTC 3 cut(s) 45, 422, 932
PflFI GACNNNGTC 1 cut(s) 1484
PflMI CCANNNNNTGG 1 cut(s) 433
PkrI GCNGC 9 cut(s) 12, 732, 988, 1128, 1747, 1957, 1960, 2347, 2535
PleI GAGTC 4 cut(s) 485, 493, 644, 2739
PmaCI CACGTG 1 cut(s) 1361
PmlI CACGTG 1 cut(s) 1361
PpsI GAGTC 4 cut(s) 485, 493, 644, 2739
Ppu21I YACGTR 1 cut(s) 1361
PscI ACATGT 1 cut(s) 2296
PsiI TTATAA 1 cut(s) 1019
Psp124BI GAGCTC 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 2357
PspCI CACGTG 1 cut(s) 1361
PspGI CCWGG 1 cut(s) 2357
PspN4I GGNNCC 3 cut(s) 14, 330, 1071
PspPI GGNCC 6 cut(s) 270, 328, 553, 1029, 1807, 1970
PstI CTGCAG 1 cut(s) 2181
PsuI RGATCY 4 cut(s) 233, 599, 1159, 2411
PsyI GACNNNGTC 1 cut(s) 1484
RsaI GTAC 4 cut(s) 1578, 1693, 1946, 2647
RsaNI GTAC 4 cut(s) 1577, 1692, 1945, 2646
RseI CAYNNNNRTG 2 cut(s) 2301, 2715
SacI GAGCTC 1 cut(s) 284
SaqAI TTAA 7 cut(s) 1182, 1767, 1914, 2205, 2223, 2231, 2640
SatI GCNGC 9 cut(s) 11, 731, 987, 1127, 1746, 1956, 1959, 2346, 2534
Sau3AI GATC 7 cut(s) 233, 599, 1159, 1600, 1891, 2411, 2629
Sau96I GGNCC 6 cut(s) 270, 328, 553, 1029, 1807, 1970
ScaI AGTACT 1 cut(s) 1946
SchI GAGTC 4 cut(s) 485, 494, 645, 2740
ScrFI CCNGG 6 cut(s) 406, 447, 552, 884, 1969, 2359
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 6 cut(s) 361, 418, 843, 1769, 2059, 2128
SfcI CTRYAG 1 cut(s) 2177
Sfr274I CTCGAG 1 cut(s) 595
SinI GGWCC 4 cut(s) 270, 328, 1029, 1807
SlaI CTCGAG 1 cut(s) 595
SmiMI CAYNNNNRTG 2 cut(s) 2301, 2715
SmlI CTYRAG 4 cut(s) 595, 1608, 1766, 2230
SmoI CTYRAG 4 cut(s) 595, 1608, 1766, 2230
SphI GCATGC 2 cut(s) 1683, 2714
SseBI AGGCCT 1 cut(s) 863
SspMI CTAG 6 cut(s) 231, 1338, 1883, 2423, 2685, 2715
SstI GAGCTC 1 cut(s) 284
StuI AGGCCT 1 cut(s) 863
StyD4I CCNGG 6 cut(s) 404, 445, 550, 882, 1967, 2357
StyI CCWWGG 2 cut(s) 1506, 1753
TaaI ACNGT 6 cut(s) 470, 798, 869, 1669, 2008, 2682
TaiI ACGT 3 cut(s) 630, 876, 1363
TaqI TCGA 8 cut(s) 365, 596, 669, 675, 851, 1044, 1169, 2287
TaqII GACCGA 1 cut(s) 2624
TatI WGTACW 4 cut(s) 1576, 1691, 1944, 2645
TauI GCSGC 4 cut(s) 13, 733, 1961, 2348
Tru1I TTAA 7 cut(s) 1182, 1767, 1914, 2205, 2223, 2231, 2640
Tru9I TTAA 7 cut(s) 1182, 1767, 1914, 2205, 2223, 2231, 2640
TscAI CASTG 6 cut(s) 536, 1039, 1269, 1398, 2221, 2480
TseFI GTSAC 4 cut(s) 869, 1356, 2056, 2314
TseI GCWGC 5 cut(s) 986, 1126, 1745, 1955, 2533
Tsp45I GTSAC 4 cut(s) 869, 1356, 2056, 2314
TspDTI ATGAA 6 cut(s) 1179, 1277, 1845, 2055, 2502, 2574
TspGWI ACGGA 2 cut(s) 825, 2594
TspRI CASTG 6 cut(s) 536, 1039, 1269, 1398, 2221, 2480
Tth111I GACNNNGTC 1 cut(s) 1484
Van91I CCANNNNNTGG 1 cut(s) 433
Vha464I CTTAAG 2 cut(s) 1766, 2230
VpaK11BI GGWCC 4 cut(s) 270, 328, 1029, 1807
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 6 cut(s) 1102, 1341, 1739, 1919, 2277, 2418
XbaI TCTAGA 1 cut(s) 2422
XceI RCATGY 4 cut(s) 1683, 2300, 2714, 2771
XcmI CCANNNNNNNNNTGG 1 cut(s) 2207
XhoI CTCGAG 1 cut(s) 595
XmiI GTMKAC 2 cut(s) 639, 2594
XmnI GAANNNNTTC 3 cut(s) 45, 422, 932
XspI CTAG 6 cut(s) 231, 1338, 1883, 2423, 2685, 2715
ZraI GACGTC 1 cut(s) 628
ZrmI AGTACT 1 cut(s) 1946
Zsp2I ATGCAT 1 cut(s) 2052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.