MD02G1205300.v1.1

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005437
Physical Location & Seq
Forward (+)
20530493 .. 20534275
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1205300.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGTTTGGAACATTTGTTTTGCCTGCATCCTATGGTTATGTGACACGCGTCCAAGCCATGCAGATGCAGTCAAATCGTTGCCTCCAGAAAGTCGTGGACCAATGGAGGGGGACACGTGTAAATATCTGGGCCGGCGCTCCCTCTCCAAGGCAACTAGTATATAAGGGCAACCAAACGAAACGAAACGAAGCAATTAGGCCGTTCTGGAATTCTCTGGTCATATTCTTCTCCTCCACTCTCTCAATCTCTCAAACCCTCGTTTCATTTCCTTCTACCAGAGGGATAAACACAAACAAAATGCAGAGTGAAAGAGACAATAAAGGTAGTGTTTCTCGGTCACGCGGATTGTTAGATAGTTTTCGGGGCTTTGGGTCCCGGAGAAGTATGTTCCCTAGCCTCTTTGGTGGAAGAGATCCATTTGATGATCCCTTCTTTAATCACCCGATTGGTAGCATGTTCGAGTCTAGTATTTTCGGTCCAAGTTCTGTTTCTAGTGATACACCGGAGAGTGGTAGAGCCAATGAAATATCAGTAGAAGAACTGAACTCTGATGATGAGGGAGGGGATGATATAGTTACTGGGGATGAGAGAGATAACTGTGGAAAGCAATCTGTTTCGAGTAAAGAACCATCTGTTGAGCATCCAGATGATGTTTTGGACGAAGAAAGGAAAAGCAAGGATGAGACATATAGAAATGACCGCAATAAGGTGGAAGGCACTCAGACCCAAGTCCGTGGTTTATCTTTTCAGACTTGCAGAGTCACATATGGTGGTGTAGATGGAGCATATTACACTTCTACGAGAACCAGAAGGGCGGGCAGTGATGGCGTGGTGCTTGAGGAGGCCAAAGAAGCAGATAAAACAACCGGTCAAGCAACACATAGAATCTCGAGAGGACTTTATGACAAGGGTCATTCTGTTACAAGGAAGCTCAAGTCAGATGGGAAGGTGGATATGTTGCAAACTTTACACAATTTAAATGAAGATGAGCTTCCTGGTTTTGAAGAAGCATGGACTGGTAATGTTGGAGGGCACTTGCCCGGCTGGAGATATGAATTTGATATGCATGGCAATACCAGTTCCTCCACTAGCAGAGAGCAGAGTAGAGGAGAGGCGTTTTCAGGAGTGGGGCTTCTTCCATCTTCTGAGCACGTGCAAAGCAGCAGAGGAATGGAATCAGACAACGCAACAAAGACTACCTCCGATGGGAGGACCAAAAGGGTTGTCAGAATCAATATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

415

Amino Acids

45.96

Weight (kDa)

6.3

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlf1IP PF10248 195 - 340 3.6e-18 Myelodysplasia-myeloid leukemia factor 1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 48, 342
AciI CCGC 3 cut(s) 342, 700, 815
AclWI GGATC 2 cut(s) 407, 419
AcsI RAATTY 2 cut(s) 208, 1055
AcvI CACGTG 2 cut(s) 116, 1153
AfiI CCNNNNNNNGG 6 cut(s) 106, 147, 509, 706, 1206, 1209
AflIII ACRYGT 3 cut(s) 46, 113, 115
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 1 cut(s) 1004
AhlI ACTAGT 1 cut(s) 154
AjnI CCWGG 1 cut(s) 994
AjuI GAANNNNNNNTTGG 2 cut(s) 472, 504
AluBI AGCT 2 cut(s) 931, 991
AluI AGCT 2 cut(s) 931, 991
Alw21I GWGCWC 1 cut(s) 1152
Alw26I GTCTC 2 cut(s) 306, 677
AlwI GGATC 2 cut(s) 407, 419
Ama87I CYCGRG 1 cut(s) 889
AoxI GGCC 3 cut(s) 129, 197, 843
ApeKI GCWGC 1 cut(s) 1161
ApoI RAATTY 2 cut(s) 208, 1055
ArsI GACNNNNNNTTYG 2 cut(s) 1209, 1241
AsiGI ACCGGT 1 cut(s) 866
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 5 cut(s) 97, 129, 372, 476, 1212
AsuC2I CCSGG 2 cut(s) 376, 1041
AsuHPI GGTGA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 889
AvaII GGWCC 4 cut(s) 97, 372, 476, 1212
BaeGI GKGCMC 1 cut(s) 1035
BbrPI CACGTG 2 cut(s) 116, 1153
Bbv12I GWGCWC 1 cut(s) 1152
BbvI GCAGC 1 cut(s) 1173
BccI CCATC 6 cut(s) 637, 773, 818, 935, 1147, 1199
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 56, 90
BciT130I CCWGG 1 cut(s) 996
BcnI CCSGG 2 cut(s) 376, 1041
BcoDI GTCTC 2 cut(s) 306, 677
BcuI ACTAGT 1 cut(s) 154
BfaI CTAG 5 cut(s) 155, 393, 465, 492, 1089
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 1 cut(s) 1162
BlsI GCNGC 1 cut(s) 1163
Bme1390I CCNGG 3 cut(s) 376, 996, 1041
Bme18I GGWCC 4 cut(s) 97, 372, 476, 1212
BmeT110I CYCGRG 1 cut(s) 889
BmgT120I GGNCC 5 cut(s) 97, 129, 372, 476, 1212
BmiI GGNNCC 2 cut(s) 373, 374
BmrFI CCNGG 3 cut(s) 376, 996, 1041
BmrI ACTGGG 1 cut(s) 588
BmsI GCATC 3 cut(s) 35, 54, 649
BmuI ACTGGG 1 cut(s) 588
BoxI GACNNNNGTC 3 cut(s) 47, 728, 909
BpmI CTGGAG 2 cut(s) 68, 1066
BpuEI CTTGAG 2 cut(s) 857, 917
BpuMI CCSGG 2 cut(s) 376, 1041
BsaAI YACGTR 2 cut(s) 116, 1153
BsaJI CCNNGG 2 cut(s) 146, 733
BsaWI WCCGGW 2 cut(s) 502, 866
Bsc4I CCNNNNNNNGG 6 cut(s) 106, 147, 509, 706, 1206, 1209
Bse118I RCCGGY 2 cut(s) 131, 866
Bse1I ACTGG 3 cut(s) 583, 1021, 1077
BseBI CCWGG 1 cut(s) 996
BseDI CCNNGG 2 cut(s) 146, 733
BseGI GGATG 5 cut(s) 26, 571, 589, 640, 685
BseLI CCNNNNNNNGG 6 cut(s) 106, 147, 509, 706, 1206, 1209
BseMII CTCAG 2 cut(s) 734, 1137
BseNI ACTGG 3 cut(s) 583, 1021, 1077
BseRI GAGGAG 3 cut(s) 220, 854, 1122
BseSI GKGCMC 1 cut(s) 1035
BseXI GCAGC 1 cut(s) 1173
Bsh1236I CGCG 2 cut(s) 48, 342
BshFI GGCC 3 cut(s) 131, 199, 845
BshTI ACCGGT 1 cut(s) 866
BsiHKAI GWGCWC 1 cut(s) 1152
BsiHKCI CYCGRG 1 cut(s) 889
BsiSI CCGG 5 cut(s) 132, 376, 503, 867, 1041
BslFI GGGAC 2 cut(s) 124, 358
BslI CCNNNNNNNGG 6 cut(s) 106, 147, 509, 706, 1206, 1209
BsmAI GTCTC 2 cut(s) 306, 677
BsmFI GGGAC 2 cut(s) 124, 358
BsnI GGCC 3 cut(s) 131, 199, 845
BsoBI CYCGRG 1 cut(s) 889
Bsp1286I GDGCHC 2 cut(s) 1035, 1152
Bsp143I GATC 2 cut(s) 412, 424
BspACI CCGC 3 cut(s) 342, 700, 815
BspANI GGCC 3 cut(s) 131, 199, 845
BspCNI CTCAG 2 cut(s) 733, 1138
BspFNI CGCG 2 cut(s) 48, 342
BspLI GGNNCC 2 cut(s) 373, 374
BspPI GGATC 2 cut(s) 407, 419
BsrFI RCCGGY 2 cut(s) 131, 866
BsrI ACTGG 3 cut(s) 583, 1021, 1077
BssAI RCCGGY 2 cut(s) 131, 866
BssECI CCNNGG 2 cut(s) 146, 733
BssMI GATC 2 cut(s) 412, 424
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 996
Bst4CI ACNGT 1 cut(s) 599
Bst6I CTCTTC 1 cut(s) 403
BstBAI YACGTR 2 cut(s) 116, 1153
BstC8I GCNNGC 3 cut(s) 24, 133, 817
BstDEI CTNAG 2 cut(s) 720, 1146
BstDSI CCRYGG 1 cut(s) 733
BstENI CCTNNNNNAGG 1 cut(s) 145
BstF5I GGATG 5 cut(s) 26, 571, 589, 640, 685
BstFNI CGCG 2 cut(s) 48, 342
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 2 cut(s) 415, 427
BstMAI GTCTC 2 cut(s) 306, 677
BstMBI GATC 2 cut(s) 412, 424
BstMWI GCNNNNNNNGC 2 cut(s) 825, 851
BstNI CCWGG 1 cut(s) 996
BstNSI RCATGY 1 cut(s) 457
BstPAI GACNNNNGTC 3 cut(s) 47, 728, 909
BstSCI CCNGG 3 cut(s) 374, 994, 1039
BstSLI GKGCMC 1 cut(s) 1035
BstUI CGCG 2 cut(s) 48, 342
BstV1I GCAGC 1 cut(s) 1173
BstX2I RGATCY 1 cut(s) 412
BstXI CCANNNNNNTGG 1 cut(s) 734
BstYI RGATCY 1 cut(s) 412
BsuRI GGCC 3 cut(s) 131, 199, 845
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 5 cut(s) 26, 571, 589, 640, 685
BtsI GCAGTG 1 cut(s) 826
BtsIMutI CAGTG 1 cut(s) 826
Cac8I GCNNGC 3 cut(s) 24, 133, 817
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 2 cut(s) 131, 866
Cfr13I GGNCC 5 cut(s) 97, 129, 372, 476, 1212
CseI GACGC 1 cut(s) 37
CspAI ACCGGT 1 cut(s) 866
CviAII CATG 4 cut(s) 58, 454, 1011, 1067
DdeI CTNAG 2 cut(s) 720, 1146
DpnI GATC 2 cut(s) 414, 426
DpnII GATC 2 cut(s) 412, 424
DraI TTTAAA 1 cut(s) 978
Eam1104I CTCTTC 1 cut(s) 403
EarI CTCTTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 146
Eco47I GGWCC 4 cut(s) 97, 372, 476, 1212
Eco72I CACGTG 2 cut(s) 116, 1153
Eco88I CYCGRG 1 cut(s) 889
EcoNI CCTNNNNNAGG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 372
EcoRI GAATTC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 994
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 1068
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 4 cut(s) 61, 457, 1014, 1070
FalI AAGNNNNNCTT 2 cut(s) 975, 1007
FaqI GGGAC 2 cut(s) 124, 358
FatI CATG 4 cut(s) 57, 453, 1010, 1066
FauI CCCGC 1 cut(s) 808
FauNDI CATATG 1 cut(s) 766
Fnu4HI GCNGC 1 cut(s) 1162
FokI GGATG 5 cut(s) 13, 578, 596, 627, 692
Fsp4HI GCNGC 1 cut(s) 1162
FspBI CTAG 5 cut(s) 155, 393, 465, 492, 1089
GlaI GCGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 1162
GsuI CTGGAG 2 cut(s) 68, 1066
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 3 cut(s) 131, 199, 845
HapII CCGG 5 cut(s) 132, 376, 503, 867, 1041
HgaI GACGC 1 cut(s) 37
HhaI GCGC 1 cut(s) 137
Hin1II CATG 4 cut(s) 61, 457, 1014, 1070
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HinfI GANTC 5 cut(s) 461, 759, 885, 1175, 1230
HpaII CCGG 5 cut(s) 132, 376, 503, 867, 1041
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 8 cut(s) 550, 723, 750, 940, 1147, 1180, 1204, 1229
Hpy188III TCNNGA 6 cut(s) 85, 205, 644, 889, 891, 1122
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 5 cut(s) 279, 439, 707, 804, 940
HpyCH4III ACNGT 1 cut(s) 599
HpyCH4IV ACGT 2 cut(s) 115, 1152
HpyCH4V TGCA 8 cut(s) 26, 61, 67, 301, 756, 961, 1066, 1156
HpyF10VI GCNNNNNNNGC 2 cut(s) 825, 851
HpyF3I CTNAG 2 cut(s) 720, 1146
HpySE526I ACGT 2 cut(s) 115, 1152
Hsp92II CATG 4 cut(s) 61, 457, 1014, 1070
HspAI GCGC 1 cut(s) 135
KflI GGGWCCC 1 cut(s) 372
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Kzo9I GATC 2 cut(s) 412, 424
LmnI GCTCC 2 cut(s) 142, 782
Lsp1109I GCAGC 1 cut(s) 1173
LweI GCATC 3 cut(s) 35, 54, 649
MaeI CTAG 5 cut(s) 155, 393, 465, 492, 1089
MaeII ACGT 2 cut(s) 115, 1152
MaeIII GTNAC 5 cut(s) 40, 336, 574, 760, 919
MalI GATC 2 cut(s) 414, 426
MboI GATC 2 cut(s) 412, 424
MboII GAAGA 8 cut(s) 217, 420, 548, 674, 995, 1016, 1127, 1134
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 2 cut(s) 1035, 1152
MluCI AATT 4 cut(s) 192, 208, 973, 1055
MluI ACGCGT 1 cut(s) 46
MlyI GAGTC 2 cut(s) 470, 768
MmeI TCCRAC 1 cut(s) 1006
Mph1103I ATGCAT 1 cut(s) 1068
MroNI GCCGGC 1 cut(s) 131
MseI TTAA 2 cut(s) 435, 977
MslI CAYNNNNRTG 2 cut(s) 62, 645
MspI CCGG 5 cut(s) 132, 376, 503, 867, 1041
MspR9I CCNGG 3 cut(s) 376, 996, 1041
MvaI CCWGG 1 cut(s) 996
MvnI CGCG 2 cut(s) 48, 342
MwoI GCNNNNNNNGC 2 cut(s) 825, 851
NaeI GCCGGC 1 cut(s) 133
NciI CCSGG 2 cut(s) 376, 1041
NdeI CATATG 1 cut(s) 766
NdeII GATC 2 cut(s) 412, 424
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 4 cut(s) 61, 457, 1014, 1070
NlaIV GGNNCC 2 cut(s) 373, 374
NmuCI GTSAC 3 cut(s) 40, 336, 760
NsiI ATGCAT 1 cut(s) 1068
NspI RCATGY 1 cut(s) 457
PaeR7I CTCGAG 1 cut(s) 889
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 3 cut(s) 885, 1175, 1230
PfoI TCCNGGA 1 cut(s) 374
PinAI ACCGGT 1 cut(s) 866
PkrI GCNGC 1 cut(s) 1163
PleI GAGTC 2 cut(s) 469, 767
PmaCI CACGTG 2 cut(s) 116, 1153
PmlI CACGTG 2 cut(s) 116, 1153
PpsI GAGTC 2 cut(s) 469, 767
Ppu21I YACGTR 2 cut(s) 116, 1153
PpuMI RGGWCCY 1 cut(s) 372
PshAI GACNNNNGTC 3 cut(s) 47, 728, 909
Psp5II RGGWCCY 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 994
PspCI CACGTG 2 cut(s) 116, 1153
PspGI CCWGG 1 cut(s) 994
PspN4I GGNNCC 2 cut(s) 373, 374
PspPI GGNCC 5 cut(s) 97, 129, 372, 476, 1212
PspPPI RGGWCCY 1 cut(s) 372
PsuI RGATCY 1 cut(s) 412
RseI CAYNNNNRTG 2 cut(s) 62, 645
SaqAI TTAA 2 cut(s) 435, 977
SatI GCNGC 1 cut(s) 1162
Sau3AI GATC 2 cut(s) 412, 424
Sau96I GGNCC 5 cut(s) 97, 129, 372, 476, 1212
SchI GAGTC 2 cut(s) 470, 768
ScrFI CCNGG 3 cut(s) 376, 996, 1041
SduI GDGCHC 2 cut(s) 1035, 1152
SetI ASST 8 cut(s) 118, 325, 711, 933, 951, 993, 1155, 1202
SfaNI GCATC 3 cut(s) 35, 54, 649
Sfr274I CTCGAG 1 cut(s) 889
SinI GGWCC 4 cut(s) 97, 372, 476, 1212
SlaI CTCGAG 1 cut(s) 889
SmiI ATTTAAAT 1 cut(s) 978
SmiMI CAYNNNNRTG 2 cut(s) 62, 645
SmlI CTYRAG 3 cut(s) 836, 889, 932
SmoI CTYRAG 3 cut(s) 836, 889, 932
SpeI ACTAGT 1 cut(s) 154
Sse9I AATT 4 cut(s) 192, 208, 973, 1055
SsiI CCGC 3 cut(s) 342, 700, 815
SspMI CTAG 5 cut(s) 155, 393, 465, 492, 1089
StyD4I CCNGG 3 cut(s) 374, 994, 1039
StyI CCWWGG 1 cut(s) 146
SwaI ATTTAAAT 1 cut(s) 978
TaaI ACNGT 1 cut(s) 599
TaiI ACGT 2 cut(s) 118, 1155
TaqI TCGA 3 cut(s) 459, 617, 890
TaqII GACCGA 2 cut(s) 324, 464
TasI AATT 4 cut(s) 192, 208, 973, 1055
TfiI GAWTC 3 cut(s) 885, 1175, 1230
Tru1I TTAA 2 cut(s) 435, 977
Tru9I TTAA 2 cut(s) 435, 977
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 3 cut(s) 40, 336, 760
TseI GCWGC 1 cut(s) 1161
Tsp45I GTSAC 3 cut(s) 40, 336, 760
TspDTI ATGAA 4 cut(s) 252, 537, 996, 1068
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 1 cut(s) 826
VpaK11BI GGWCC 4 cut(s) 97, 372, 476, 1212
XagI CCTNNNNNAGG 1 cut(s) 145
XapI RAATTY 2 cut(s) 208, 1055
XceI RCATGY 1 cut(s) 457
XhoI CTCGAG 1 cut(s) 889
XspI CTAG 5 cut(s) 155, 393, 465, 492, 1089
Zsp2I ATGCAT 1 cut(s) 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.