Rw0G018810

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00847
OG0005437
Physical Location & Seq
Forward (+)
178859 .. 183558
4700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G018810.1

Sequence Viewer

Length: 870 bp
ATGCAAAGCGAAAGGCAAGGCAGACGCGGTTTCTCTCGCTCAGATGACCTGTTAAATAGTTTCCCGGGATTTGGATCTCGTAGAAGCATGTTGCCTAGCCTCTTTAGTGGAAGGGATCCATTTGATGATCCCTTCTTCAGTCGGCCGCTTGGAGACATGTTTGAGTCTAGCATGTTCCGTCCAAGTGCTGCTTCTAGTGATACACCAAAGAGTGGTAGAGATGGGGGAATCATAGTAGAAGAACTAGACTCTGATGATGATGGAGAGGAAGATGAGGTTACTGGAGATGGGAGAGATAAGAGGAAGCAACGAACTGTGTCAAGCGAAGAGCCATCTGTTGAGCATCCGGATGATGATGATATGGATGAAGAGACAGGCAAGAACACGAGTAAAAGAGGTGATTTCAACAAGGTGCAAGGCACACAGCCCCAGGGTCGTAGTGCCTCTGTTCAGAGTTGCAGAGTCACATATGGTGGTGTAGATGGAGCATATTACACTTCTACAAGAACCAGAAGGGCAGGCAATGATGGCGTGAAGATTGAGGAGACCAAAGAAGCAGATAGGACAACTGGTCGAGCAACACATAAGATATCTAGAGGAATTCATGATAAGGGTCATTCTGTTACAAGGAAGCTTAACCCAGATGGAAAGGTGGATGTGTTGCAAACTTTACACAATCTGAATGAAGATGAGCTTGCTGGTTTTGAAGAAGCATGGAATGGTAATGTTAAGGTGGACTTACCTGGCTGGAAGAATTTGACAACCTTGCAAGCACAAAGCTCCAGAGGGATCAGACCAGACAATGTTATTGTCTGCAAGACTACTACTGATGGGAGAACCAAAAAGGTTGTTAGAATCAATATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

289

Amino Acids

31.98

Weight (kDa)

5.45

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlf1IP PF10248 93 - 241 5.4e-21 Myelodysplasia-myeloid leukemia factor 1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 212
AccII CGCG 1 cut(s) 27
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 2 cut(s) 27, 146
AclWI GGATC 5 cut(s) 82, 110, 122, 123, 797
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 2 cut(s) 600, 754
AcuI CTGAAG 1 cut(s) 121
AfiI CCNNNNNNNGG 2 cut(s) 71, 212
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 2 cut(s) 406, 707
AhdI GACNNNNNGTC 1 cut(s) 570
AjnI CCWGG 2 cut(s) 429, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 3 cut(s) 634, 694, 780
AluI AGCT 3 cut(s) 634, 694, 780
Alw26I GTCTC 3 cut(s) 147, 365, 539
AlwI GGATC 5 cut(s) 82, 110, 122, 123, 797
Ama87I CYCGRG 1 cut(s) 64
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 2 cut(s) 600, 754
AsuC2I CCSGG 2 cut(s) 65, 66
AsuHPI GGTGA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 64
BamHI GGATCC 1 cut(s) 115
BauI CACGAG 1 cut(s) 385
BbvI GCAGC 1 cut(s) 175
BccI CCATC 8 cut(s) 215, 254, 281, 340, 476, 521, 638, 824
BciT130I CCWGG 2 cut(s) 431, 744
BcnI CCSGG 2 cut(s) 65, 66
BcoDI GTCTC 3 cut(s) 147, 365, 539
BfaI CTAG 5 cut(s) 96, 168, 195, 245, 594
BisI GCNGC 2 cut(s) 146, 189
BlsI GCNGC 2 cut(s) 147, 190
Bme1390I CCNGG 4 cut(s) 65, 66, 431, 744
BmeRI GACNNNNNGTC 1 cut(s) 570
BmeT110I CYCGRG 1 cut(s) 64
BmiI GGNNCC 1 cut(s) 117
BmrFI CCNGG 4 cut(s) 65, 66, 431, 744
BmsI GCATC 1 cut(s) 352
BpmI CTGGAG 2 cut(s) 303, 766
BpuMI CCSGG 2 cut(s) 65, 66
BsaBI GATNNNNATC 1 cut(s) 73
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 3 cut(s) 64, 429, 430
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 212
Bse1I ACTGG 2 cut(s) 286, 574
Bse3DI GCAATG 1 cut(s) 529
Bse8I GATNNNNATC 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 2 cut(s) 431, 744
BseDI CCNNGG 3 cut(s) 64, 429, 430
BseGI GGATG 4 cut(s) 343, 355, 370, 661
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 2 cut(s) 71, 212
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 2 cut(s) 286, 574
BseRI GAGGAG 1 cut(s) 557
BseX3I CGGCCG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 146
BshFI GGCC 1 cut(s) 145
BsiEI CGRYCG 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 64
BsiSI CCGG 2 cut(s) 65, 347
BslI CCNNNNNNNGG 2 cut(s) 71, 212
BsmAI GTCTC 3 cut(s) 147, 365, 539
BsnI GGCC 1 cut(s) 145
Bso31I GGTCTC 1 cut(s) 539
BsoBI CYCGRG 1 cut(s) 64
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 4 cut(s) 74, 115, 127, 789
BspACI CCGC 2 cut(s) 27, 146
BspANI GGCC 1 cut(s) 145
BspCNI CTCAG 1 cut(s) 53
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 1 cut(s) 117
BspPI GGATC 5 cut(s) 82, 110, 122, 123, 797
BspQI GCTCTTC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 529
BsrI ACTGG 2 cut(s) 286, 574
BssECI CCNNGG 3 cut(s) 64, 429, 430
BssMI GATC 4 cut(s) 74, 115, 127, 789
BssSI CACGAG 1 cut(s) 385
Bst2BI CACGAG 1 cut(s) 385
Bst2UI CCWGG 2 cut(s) 431, 744
Bst4CI ACNGT 1 cut(s) 316
Bst6I CTCTTC 2 cut(s) 321, 363
BstC8I GCNNGC 3 cut(s) 520, 696, 771
BstDEI CTNAG 1 cut(s) 40
BstF5I GGATG 4 cut(s) 343, 355, 370, 661
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 4 cut(s) 77, 118, 130, 792
BstMAI GTCTC 3 cut(s) 147, 365, 539
BstMBI GATC 4 cut(s) 74, 115, 127, 789
BstMCI CGRYCG 1 cut(s) 146
BstMWI GCNNNNNNNGC 1 cut(s) 528
BstNI CCWGG 2 cut(s) 431, 744
BstNSI RCATGY 3 cut(s) 91, 160, 175
BstSCI CCNGG 4 cut(s) 63, 64, 429, 742
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 2 cut(s) 74, 115
BstYI RGATCY 2 cut(s) 74, 115
BstZI CGGCCG 1 cut(s) 143
BsuRI GGCC 1 cut(s) 145
BtsCI GGATG 4 cut(s) 343, 355, 370, 661
Cac8I GCNNGC 3 cut(s) 520, 696, 771
CciI TCATGA 1 cut(s) 604
Cfr9I CCCGGG 1 cut(s) 64
CseI GACGC 1 cut(s) 33
CviAII CATG 5 cut(s) 88, 157, 172, 605, 714
CviJI RGCY 8 cut(s) 99, 145, 331, 427, 634, 694, 747, 780
CviKI_1 RGCY 8 cut(s) 99, 145, 331, 427, 634, 694, 747, 780
DdeI CTNAG 1 cut(s) 40
DpnI GATC 4 cut(s) 76, 117, 129, 791
DpnII GATC 4 cut(s) 74, 115, 127, 789
DriI GACNNNNNGTC 1 cut(s) 570
EaeI YGGCCR 1 cut(s) 143
EagI CGGCCG 1 cut(s) 143
Eam1104I CTCTTC 2 cut(s) 321, 363
Eam1105I GACNNNNNGTC 1 cut(s) 570
EarI CTCTTC 2 cut(s) 321, 363
EclXI CGGCCG 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 539
Eco32I GATATC 1 cut(s) 591
Eco52I CGGCCG 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 600
EcoRII CCWGG 2 cut(s) 429, 742
EcoRV GATATC 1 cut(s) 591
FaeI CATG 5 cut(s) 91, 160, 175, 608, 717
FalI AAGNNNNNCTT 6 cut(s) 175, 207, 678, 710, 722, 754
FatI CATG 5 cut(s) 87, 156, 171, 604, 713
FauNDI CATATG 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 146, 189
FokI GGATG 4 cut(s) 330, 362, 377, 668
Fsp4HI GCNGC 2 cut(s) 146, 189
FspBI CTAG 5 cut(s) 96, 168, 195, 245, 594
GluI GCNGC 2 cut(s) 146, 189
GsuI CTGGAG 2 cut(s) 303, 766
HaeIII GGCC 1 cut(s) 145
HapII CCGG 2 cut(s) 65, 347
HgaI GACGC 1 cut(s) 33
Hin1II CATG 5 cut(s) 91, 160, 175, 608, 717
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 164, 228, 248, 462, 855
HpaII CCGG 2 cut(s) 65, 347
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 5 cut(s) 43, 253, 453, 681, 794
Hpy188III TCNNGA 4 cut(s) 347, 594, 605, 783
Hpy8I GTNNAC 1 cut(s) 736
HpyAV CCTTC 3 cut(s) 105, 142, 507
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4V TGCA 6 cut(s) 4, 415, 459, 664, 769, 816
HpyF10VI GCNNNNNNNGC 1 cut(s) 528
HpyF3I CTNAG 1 cut(s) 40
Hsp92II CATG 5 cut(s) 91, 160, 175, 608, 717
Kpn2I TCCGGA 1 cut(s) 346
Kzo9I GATC 4 cut(s) 74, 115, 127, 789
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 485, 785
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 1 cut(s) 352
MaeI CTAG 5 cut(s) 96, 168, 195, 245, 594
MaeIII GTNAC 3 cut(s) 277, 463, 622
MalI GATC 4 cut(s) 76, 117, 129, 791
MboI GATC 4 cut(s) 74, 115, 127, 789
MboII GAAGA 9 cut(s) 127, 251, 281, 338, 380, 547, 698, 719, 763
MflI RGATCY 2 cut(s) 74, 115
MluCI AATT 2 cut(s) 600, 754
MlyI GAGTC 3 cut(s) 173, 242, 471
MnlI CCTC 9 cut(s) 110, 259, 268, 294, 389, 454, 535, 590, 779
MroI TCCGGA 1 cut(s) 346
MseI TTAA 3 cut(s) 53, 636, 729
MslI CAYNNNNRTG 1 cut(s) 348
MspI CCGG 2 cut(s) 65, 347
MspR9I CCNGG 4 cut(s) 65, 66, 431, 744
MvaI CCWGG 2 cut(s) 431, 744
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 528
NciI CCSGG 2 cut(s) 65, 66
NdeI CATATG 1 cut(s) 469
NdeII GATC 4 cut(s) 74, 115, 127, 789
NlaIII CATG 5 cut(s) 91, 160, 175, 608, 717
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 1 cut(s) 463
NspI RCATGY 3 cut(s) 91, 160, 175
PagI TCATGA 1 cut(s) 604
PasI CCCWGGG 1 cut(s) 430
PciI ACATGT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 228, 855
PflMI CCANNNNNTGG 1 cut(s) 212
PkrI GCNGC 2 cut(s) 147, 190
PleI GAGTC 3 cut(s) 172, 242, 470
PpsI GAGTC 3 cut(s) 172, 242, 470
PscI ACATGT 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 429, 742
PspGI CCWGG 2 cut(s) 429, 742
PspN4I GGNNCC 1 cut(s) 117
PsuI RGATCY 2 cut(s) 74, 115
RseI CAYNNNNRTG 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 3 cut(s) 53, 636, 729
SatI GCNGC 2 cut(s) 146, 189
Sau3AI GATC 4 cut(s) 74, 115, 127, 789
SchI GAGTC 3 cut(s) 173, 242, 471
ScrFI CCNGG 4 cut(s) 65, 66, 431, 744
SfaNI GCATC 1 cut(s) 352
SmaI CCCGGG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 348
Sse9I AATT 2 cut(s) 600, 754
SsiI CCGC 2 cut(s) 27, 146
SspMI CTAG 5 cut(s) 96, 168, 195, 245, 594
StyD4I CCNGG 4 cut(s) 63, 64, 429, 742
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 574
TasI AATT 2 cut(s) 600, 754
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 2 cut(s) 228, 855
Tru1I TTAA 3 cut(s) 53, 636, 729
Tru9I TTAA 3 cut(s) 53, 636, 729
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 3 cut(s) 381, 593, 699
TspGWI ACGGA 1 cut(s) 167
TspMI CCCGGG 1 cut(s) 64
Van91I CCANNNNNTGG 1 cut(s) 212
XapI RAATTY 2 cut(s) 600, 754
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 3 cut(s) 91, 160, 175
XmaI CCCGGG 1 cut(s) 64
XspI CTAG 5 cut(s) 96, 168, 195, 245, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.