MD07G1121800.v1.1

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005437
Physical Location & Seq
Forward (+)
15597980 .. 15600680
2701 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1121800.v1.1.491

Sequence Viewer

Length: 786 bp
ATGCAAAGTGAAAGAGACACTAGAGATGGTGTTTCTCGATCACATGGATTGTTTGATAGTTTCCAGGGATTTGGGTCCAGAAGAAGCATGTTCCCTAGCCTTTTTGGTGGAAGGGATCCATTTGGTGATCCGTTCTTCAGTCGCCCGATTGGTAGCATCTTCGAGTCTAGTATGTTCAGTCCAAGTTCCGTTTCTAGTGATACACCGGGGAGTGGTAGAGACAACAAAATATCAGTTTCAGGCAAAGAACCATCTATTGACCATCCAGATGATGTTGTGGACGAGAGGAAAGGCAAGGATGTGACGTATAGAAATGACCGCAACAATGTGGAAGGCGCACAGTCTCAGGGCCATAATTTCTCTGTTCAGACTTGCAGAGTCACATGTGGTGATGTAGATGGAGCGTATTACACTTCTACAAGAACTAGAAGGGCAGGCGGTGATGGCGTAGCGCTTGAGGAGACCAAAGAAGCAGATAAGACAACTGGTCAAGCAACACGTAGGGTTTCGAGAGGACTTCATGACAAGGGCCATTCTGTTACAAGGAAGCTTACGTCAGATGGAAGGGTGGATGTGTTGCAAACTTTACACAATTTAAATGAAGATGAGCTTCCTGGTTTTGAAGTAGCATGGAATGGTAATGTTGAAGGTCACTTGCCTAGCTGGAAACATGAGTTTGATATGCATGGTTCCGGTAGCAGAGAACAGATGGGAAATGCGTTTTTGGCAGGGGCTTCTTCCACCTTCAGAGCAAACGCAAAGCACCAGAGGAATGGGATCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

262

Amino Acids

28.62

Weight (kDa)

6.56

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlf1IP PF10248 41 - 212 1.8e-15 Myelodysplasia-myeloid leukemia factor 1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 319, 438
AclWI GGATC 4 cut(s) 110, 122, 123, 785
AcuI CTGAAG 2 cut(s) 121, 730
AfeI AGCGCT 1 cut(s) 453
AfiI CCNNNNNNNGG 1 cut(s) 212
AflIII ACRYGT 2 cut(s) 383, 497
AgsI TTSAA 2 cut(s) 623, 647
AhdI GACNNNNNGTC 1 cut(s) 486
AjnI CCWGG 2 cut(s) 63, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 3 cut(s) 550, 610, 663
AluI AGCT 3 cut(s) 550, 610, 663
Alw26I GTCTC 4 cut(s) 9, 213, 348, 455
AlwI GGATC 4 cut(s) 110, 122, 123, 785
Aor51HI AGCGCT 1 cut(s) 453
AoxI GGCC 2 cut(s) 349, 529
AspLEI GCGC 2 cut(s) 338, 454
AspS9I GGNCC 3 cut(s) 75, 349, 529
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 3 cut(s) 137, 401, 452
AvaII GGWCC 1 cut(s) 75
BamHI GGATCC 1 cut(s) 115
BccI CCATC 7 cut(s) 20, 259, 270, 392, 437, 554, 703
BciT130I CCWGG 2 cut(s) 65, 615
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 4 cut(s) 9, 213, 348, 455
BfaI CTAG 6 cut(s) 21, 96, 168, 195, 426, 660
BfoI RGCGCY 1 cut(s) 455
Bme1390I CCNGG 3 cut(s) 65, 207, 615
Bme18I GGWCC 1 cut(s) 75
BmeRI GACNNNNNGTC 1 cut(s) 486
BmgT120I GGNCC 3 cut(s) 75, 349, 529
BmiI GGNNCC 3 cut(s) 76, 117, 691
BmrFI CCNGG 3 cut(s) 65, 207, 615
BmsI GCATC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 207
BsaAI YACGTR 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 2 cut(s) 64, 206
BsaWI WCCGGW 1 cut(s) 692
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse1I ACTGG 1 cut(s) 490
BseBI CCWGG 2 cut(s) 65, 615
BseDI CCNNGG 2 cut(s) 64, 206
BseGI GGATG 3 cut(s) 262, 304, 577
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 473
BshFI GGCC 2 cut(s) 351, 531
BsiSI CCGG 2 cut(s) 206, 693
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 4 cut(s) 9, 213, 348, 455
BsnI GGCC 2 cut(s) 351, 531
Bso31I GGTCTC 1 cut(s) 455
Bsp143I GATC 4 cut(s) 38, 115, 127, 777
BspACI CCGC 2 cut(s) 319, 438
BspANI GGCC 2 cut(s) 351, 531
BspCNI CTCAG 1 cut(s) 358
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 3 cut(s) 76, 117, 691
BspPI GGATC 4 cut(s) 110, 122, 123, 785
BspTNI GGTCTC 1 cut(s) 455
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 64, 206
BssMI GATC 4 cut(s) 38, 115, 127, 777
Bst2UI CCWGG 2 cut(s) 65, 615
Bst4CI ACNGT 1 cut(s) 342
BstBAI YACGTR 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 436
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 3 cut(s) 262, 304, 577
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 2 cut(s) 338, 454
BstKTI GATC 4 cut(s) 41, 118, 130, 780
BstMAI GTCTC 4 cut(s) 9, 213, 348, 455
BstMBI GATC 4 cut(s) 38, 115, 127, 777
BstMWI GCNNNNNNNGC 2 cut(s) 444, 725
BstNI CCWGG 2 cut(s) 65, 615
BstNSI RCATGY 2 cut(s) 91, 387
BstSCI CCNGG 3 cut(s) 63, 205, 613
BstX2I RGATCY 1 cut(s) 115
BstXI CCANNNNNNTGG 2 cut(s) 71, 773
BstYI RGATCY 1 cut(s) 115
BsuRI GGCC 2 cut(s) 351, 531
BtsCI GGATG 3 cut(s) 262, 304, 577
Cac8I GCNNGC 1 cut(s) 436
CciI TCATGA 1 cut(s) 520
CfoI GCGC 2 cut(s) 338, 454
Cfr13I GGNCC 3 cut(s) 75, 349, 529
CviAII CATG 7 cut(s) 44, 88, 384, 521, 630, 671, 686
CviJI RGCY 7 cut(s) 99, 351, 531, 550, 610, 663, 734
CviKI_1 RGCY 7 cut(s) 99, 351, 531, 550, 610, 663, 734
DdeI CTNAG 1 cut(s) 345
DpnI GATC 4 cut(s) 40, 117, 129, 779
DpnII GATC 4 cut(s) 38, 115, 127, 777
DraI TTTAAA 1 cut(s) 597
DriI GACNNNNNGTC 1 cut(s) 486
Eam1105I GACNNNNNGTC 1 cut(s) 486
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 75
Eco47III AGCGCT 1 cut(s) 453
Eco57I CTGAAG 2 cut(s) 121, 730
EcoRII CCWGG 2 cut(s) 63, 613
EcoT22I ATGCAT 1 cut(s) 687
FaeI CATG 7 cut(s) 47, 91, 387, 524, 633, 674, 689
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FatI CATG 7 cut(s) 43, 87, 383, 520, 629, 670, 685
FokI GGATG 3 cut(s) 249, 311, 584
FspBI CTAG 6 cut(s) 21, 96, 168, 195, 426, 660
GlaI GCGC 2 cut(s) 337, 453
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 2 cut(s) 351, 531
HapII CCGG 2 cut(s) 206, 693
HhaI GCGC 2 cut(s) 338, 454
Hin1II CATG 7 cut(s) 47, 91, 387, 524, 633, 674, 689
Hin6I GCGC 2 cut(s) 336, 452
HinP1I GCGC 2 cut(s) 336, 452
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 2 cut(s) 164, 378
HpaII CCGG 2 cut(s) 206, 693
HphI GGTGA 3 cut(s) 137, 401, 452
Hpy166II GTNNAC 1 cut(s) 280
Hpy188I TCNGA 4 cut(s) 369, 559, 749, 782
Hpy188III TCNNGA 5 cut(s) 36, 78, 266, 510, 521
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 6 cut(s) 105, 326, 423, 558, 641, 754
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 3 cut(s) 305, 499, 554
HpyCH4V TGCA 4 cut(s) 4, 375, 580, 685
HpyF10VI GCNNNNNNNGC 2 cut(s) 444, 725
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 3 cut(s) 305, 499, 554
Hsp92II CATG 7 cut(s) 47, 91, 387, 524, 633, 674, 689
HspAI GCGC 2 cut(s) 336, 452
Kzo9I GATC 4 cut(s) 38, 115, 127, 777
LmnI GCTCC 1 cut(s) 401
LweI GCATC 1 cut(s) 165
MaeI CTAG 6 cut(s) 21, 96, 168, 195, 426, 660
MaeII ACGT 3 cut(s) 305, 499, 554
MaeIII GTNAC 4 cut(s) 301, 379, 538, 650
MalI GATC 4 cut(s) 40, 117, 129, 779
MboI GATC 4 cut(s) 38, 115, 127, 777
MboII GAAGA 5 cut(s) 93, 127, 151, 614, 729
MflI RGATCY 1 cut(s) 115
MluCI AATT 2 cut(s) 355, 592
MlyI GAGTC 2 cut(s) 173, 387
MnlI CCTC 4 cut(s) 279, 451, 506, 762
Mph1103I ATGCAT 1 cut(s) 687
MseI TTAA 1 cut(s) 596
MslI CAYNNNNRTG 1 cut(s) 267
MspI CCGG 2 cut(s) 206, 693
MspR9I CCNGG 3 cut(s) 65, 207, 615
MvaI CCWGG 2 cut(s) 65, 615
MwoI GCNNNNNNNGC 2 cut(s) 444, 725
NciI CCSGG 1 cut(s) 207
NdeII GATC 4 cut(s) 38, 115, 127, 777
NlaIII CATG 7 cut(s) 47, 91, 387, 524, 633, 674, 689
NlaIV GGNNCC 3 cut(s) 76, 117, 691
NmuCI GTSAC 3 cut(s) 301, 379, 650
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 2 cut(s) 91, 387
PagI TCATGA 1 cut(s) 520
PciI ACATGT 1 cut(s) 383
PleI GAGTC 2 cut(s) 172, 386
PpsI GAGTC 2 cut(s) 172, 386
Ppu21I YACGTR 1 cut(s) 500
PscI ACATGT 1 cut(s) 383
Psp6I CCWGG 2 cut(s) 63, 613
PspGI CCWGG 2 cut(s) 63, 613
PspN4I GGNNCC 3 cut(s) 76, 117, 691
PspPI GGNCC 3 cut(s) 75, 349, 529
PsuI RGATCY 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 596
Sau3AI GATC 4 cut(s) 38, 115, 127, 777
Sau96I GGNCC 3 cut(s) 75, 349, 529
SchI GAGTC 2 cut(s) 173, 387
ScrFI CCNGG 3 cut(s) 65, 207, 615
SetI ASST 8 cut(s) 308, 502, 552, 557, 612, 652, 665, 746
SfaNI GCATC 1 cut(s) 165
SinI GGWCC 1 cut(s) 75
SmiI ATTTAAAT 1 cut(s) 597
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 2 cut(s) 355, 592
SsiI CCGC 2 cut(s) 319, 438
SspMI CTAG 6 cut(s) 21, 96, 168, 195, 426, 660
StyD4I CCNGG 3 cut(s) 63, 205, 613
SwaI ATTTAAAT 1 cut(s) 597
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 3 cut(s) 308, 502, 557
TaqI TCGA 3 cut(s) 37, 162, 509
TasI AATT 2 cut(s) 355, 592
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TseFI GTSAC 3 cut(s) 301, 379, 650
Tsp45I GTSAC 3 cut(s) 301, 379, 650
TspDTI ATGAA 2 cut(s) 509, 615
TspGWI ACGGA 2 cut(s) 120, 178
VpaK11BI GGWCC 1 cut(s) 75
XceI RCATGY 2 cut(s) 91, 387
XspI CTAG 6 cut(s) 21, 96, 168, 195, 426, 660
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.