RchiOBHm_Chr1g0350241

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005437
Physical Location & Seq
Reverse (-)
43417832 .. 43421081
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57612

Sequence Viewer

Length: 918 bp
ATGCAAAGCGAAAGGCAAGGCAGACGCGGTTTCTCTCGCTCAGATGACCTGTTAAATAGTTTCCCGGGATTTGGATCTCGTAGAAGCATGTTGCCTAGCCTCTTCAGTGGAAGGGATCCATTTGATGATCCCTTCTTCAGTCGGCCGCTTGGAGACATGTTTGAGTCTAGCATGTTCCGTCCAAGTGCTGCTTCTAGTGATACACCAAAGAGTGGTAGAGATGGGGGAATCATAGTAGAAGAACTAGACTCTGATGATGACGGAGAGGAAGATGAGGTTACTGGAGATGGGAGAGATAAGAGGAAGCAACGAACTGTGTCAAGCGAAGAGCCATCTGTTGAGCATCCTGATGATGATGATATGGATGAAGAGACAGGCAAGAACACGAGTAAAAGAGGTGATTTCAACAAGGTGCAAGGCACACAGCCCCAGGGTCATAGTGCCTCTGTTCAGAGTTGCAGAGTCACATATGGTGGTGTAGATGGAGCATATTACACTTCTACAAGAACCAGAAGGGCAGGCAATGATGGCGTGAAGATTGAGGAGACCAAAGAAGCAGATAGGACAACTGGTCGAGCAACACATAAGATATCTAGAGGAATTCATGATAAGGGTCATTCTGTTACAAGGAAGCTTAACCCAGATGGAAAGGTGGATATGTTGCAAACTTTACACAATCTGAATGAAGATGAGCTTGCTGGTTTTGAAGAAGCATGGAATGGTAATGTTAAGGTGGACTTACCTGGCTGGAAAGAATTTGACAACCTTGGTGAACGGAAGGGCAATGCACCTTGGAGTGCTGGGCTCCTTCAATCTTCAGAGCAACCACAAAGCTCCAGATGGATTAGACCAGGGAATGTAGCCGGGACTACTTCTGATGGGAGAACCAAGAAGGTTGTTAGAATCAATATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

305

Amino Acids

33.7

Weight (kDa)

5.27

Isoelectric Point (pI)

56.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlf1IP PF10248 93 - 241 7.4e-22 Myelodysplasia-myeloid leukemia factor 1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 212
AccII CGCG 1 cut(s) 27
AciI CCGC 2 cut(s) 27, 146
AclWI GGATC 4 cut(s) 82, 110, 122, 123
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 2 cut(s) 600, 755
AcuI CTGAAG 3 cut(s) 88, 121, 801
AfiI CCNNNNNNNGG 2 cut(s) 71, 212
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 3 cut(s) 406, 707, 812
AhdI GACNNNNNGTC 1 cut(s) 570
AjnI CCWGG 3 cut(s) 429, 742, 850
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 3 cut(s) 634, 694, 834
AluI AGCT 3 cut(s) 634, 694, 834
Alw26I GTCTC 3 cut(s) 147, 365, 539
AlwI GGATC 4 cut(s) 82, 110, 122, 123
Ama87I CYCGRG 1 cut(s) 64
AoxI GGCC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 2 cut(s) 600, 755
AsuC2I CCSGG 3 cut(s) 65, 66, 865
AsuHPI GGTGA 2 cut(s) 410, 782
AvaI CYCGRG 1 cut(s) 64
BamHI GGATCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 807
BauI CACGAG 1 cut(s) 385
BbvI GCAGC 1 cut(s) 175
BccI CCATC 8 cut(s) 215, 281, 340, 476, 521, 638, 834, 872
BciT130I CCWGG 3 cut(s) 431, 744, 852
BcnI CCSGG 3 cut(s) 65, 66, 865
BcoDI GTCTC 3 cut(s) 147, 365, 539
BfaI CTAG 5 cut(s) 96, 168, 195, 245, 594
BisI GCNGC 2 cut(s) 146, 189
BlsI GCNGC 2 cut(s) 147, 190
Bme1390I CCNGG 6 cut(s) 65, 66, 431, 744, 852, 865
BmeRI GACNNNNNGTC 1 cut(s) 570
BmeT110I CYCGRG 1 cut(s) 64
BmiI GGNNCC 2 cut(s) 117, 806
BmrFI CCNGG 6 cut(s) 65, 66, 431, 744, 852, 865
BmsI GCATC 1 cut(s) 352
BpmI CTGGAG 2 cut(s) 303, 820
BpuMI CCSGG 3 cut(s) 65, 66, 865
BsaBI GATNNNNATC 1 cut(s) 73
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 6 cut(s) 64, 429, 430, 766, 791, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 212
Bse1I ACTGG 2 cut(s) 286, 574
Bse3DI GCAATG 2 cut(s) 529, 790
Bse8I GATNNNNATC 1 cut(s) 73
BseBI CCWGG 3 cut(s) 431, 744, 852
BseDI CCNNGG 6 cut(s) 64, 429, 430, 766, 791, 851
BseGI GGATG 2 cut(s) 343, 370
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 2 cut(s) 71, 212
BseMI GCAATG 2 cut(s) 529, 790
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 2 cut(s) 286, 574
BseRI GAGGAG 1 cut(s) 557
BseX3I CGGCCG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 175
BseYI CCCAGC 1 cut(s) 800
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 146
BshFI GGCC 1 cut(s) 145
BsiEI CGRYCG 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 64
BsiSI CCGG 2 cut(s) 65, 864
BslFI GGGAC 1 cut(s) 880
BslI CCNNNNNNNGG 2 cut(s) 71, 212
BsmAI GTCTC 3 cut(s) 147, 365, 539
BsmFI GGGAC 1 cut(s) 880
BsnI GGCC 1 cut(s) 145
Bso31I GGTCTC 1 cut(s) 539
BsoBI CYCGRG 1 cut(s) 64
Bsp1286I GDGCHC 1 cut(s) 807
Bsp143I GATC 3 cut(s) 74, 115, 127
BspACI CCGC 2 cut(s) 27, 146
BspANI GGCC 1 cut(s) 145
BspCNI CTCAG 1 cut(s) 53
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 117, 806
BspPI GGATC 4 cut(s) 82, 110, 122, 123
BspQI GCTCTTC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 539
BsrDI GCAATG 2 cut(s) 529, 790
BsrI ACTGG 2 cut(s) 286, 574
BssECI CCNNGG 6 cut(s) 64, 429, 430, 766, 791, 851
BssMI GATC 3 cut(s) 74, 115, 127
BssSI CACGAG 1 cut(s) 385
BssT1I CCWWGG 2 cut(s) 766, 791
Bst2BI CACGAG 1 cut(s) 385
Bst2UI CCWGG 3 cut(s) 431, 744, 852
Bst4CI ACNGT 1 cut(s) 316
Bst6I CTCTTC 3 cut(s) 107, 321, 363
BstC8I GCNNGC 2 cut(s) 520, 696
BstDEI CTNAG 1 cut(s) 40
BstF5I GGATG 2 cut(s) 343, 370
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 3 cut(s) 77, 118, 130
BstMAI GTCTC 3 cut(s) 147, 365, 539
BstMBI GATC 3 cut(s) 74, 115, 127
BstMCI CGRYCG 1 cut(s) 146
BstMWI GCNNNNNNNGC 1 cut(s) 528
BstNI CCWGG 3 cut(s) 431, 744, 852
BstNSI RCATGY 3 cut(s) 91, 160, 175
BstSCI CCNGG 6 cut(s) 63, 64, 429, 742, 850, 863
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 2 cut(s) 74, 115
BstYI RGATCY 2 cut(s) 74, 115
BstZI CGGCCG 1 cut(s) 143
BsuRI GGCC 1 cut(s) 145
BtsCI GGATG 2 cut(s) 343, 370
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 2 cut(s) 520, 696
CciI TCATGA 1 cut(s) 604
Cfr9I CCCGGG 1 cut(s) 64
CseI GACGC 1 cut(s) 33
CviAII CATG 5 cut(s) 88, 157, 172, 605, 714
DdeI CTNAG 1 cut(s) 40
DpnI GATC 3 cut(s) 76, 117, 129
DpnII GATC 3 cut(s) 74, 115, 127
DriI GACNNNNNGTC 1 cut(s) 570
EaeI YGGCCR 1 cut(s) 143
EagI CGGCCG 1 cut(s) 143
Eam1104I CTCTTC 3 cut(s) 107, 321, 363
Eam1105I GACNNNNNGTC 1 cut(s) 570
EarI CTCTTC 3 cut(s) 107, 321, 363
EclXI CGGCCG 1 cut(s) 143
Eco130I CCWWGG 2 cut(s) 766, 791
Eco24I GRGCYC 1 cut(s) 807
Eco31I GGTCTC 1 cut(s) 539
Eco32I GATATC 1 cut(s) 591
Eco52I CGGCCG 1 cut(s) 143
Eco57I CTGAAG 3 cut(s) 88, 121, 801
Eco88I CYCGRG 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 600
EcoRII CCWGG 3 cut(s) 429, 742, 850
EcoRV GATATC 1 cut(s) 591
EcoT14I CCWWGG 2 cut(s) 766, 791
EcoT38I GRGCYC 1 cut(s) 807
ErhI CCWWGG 2 cut(s) 766, 791
FaeI CATG 5 cut(s) 91, 160, 175, 608, 717
FalI AAGNNNNNCTT 6 cut(s) 175, 207, 678, 710, 722, 754
FaqI GGGAC 1 cut(s) 880
FatI CATG 5 cut(s) 87, 156, 171, 604, 713
FauNDI CATATG 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 146, 189
FokI GGATG 2 cut(s) 330, 377
FriOI GRGCYC 1 cut(s) 807
Fsp4HI GCNGC 2 cut(s) 146, 189
FspBI CTAG 5 cut(s) 96, 168, 195, 245, 594
GluI GCNGC 2 cut(s) 146, 189
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 2 cut(s) 303, 820
HaeIII GGCC 1 cut(s) 145
HapII CCGG 2 cut(s) 65, 864
HgaI GACGC 1 cut(s) 33
Hin1II CATG 5 cut(s) 91, 160, 175, 608, 717
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 164, 228, 248, 462, 903
HpaII CCGG 2 cut(s) 65, 864
HphI GGTGA 2 cut(s) 410, 782
Hpy166II GTNNAC 2 cut(s) 736, 773
Hpy188I TCNGA 6 cut(s) 43, 253, 453, 681, 820, 877
Hpy188III TCNNGA 4 cut(s) 347, 594, 605, 837
Hpy8I GTNNAC 2 cut(s) 736, 773
HpyAV CCTTC 6 cut(s) 105, 142, 507, 772, 818, 886
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4V TGCA 5 cut(s) 4, 415, 459, 664, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 528
HpyF3I CTNAG 1 cut(s) 40
Hsp92II CATG 5 cut(s) 91, 160, 175, 608, 717
Kzo9I GATC 3 cut(s) 74, 115, 127
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 3 cut(s) 485, 810, 839
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 1 cut(s) 352
MaeI CTAG 5 cut(s) 96, 168, 195, 245, 594
MaeIII GTNAC 3 cut(s) 277, 463, 622
MalI GATC 3 cut(s) 76, 117, 129
MboI GATC 3 cut(s) 74, 115, 127
MflI RGATCY 2 cut(s) 74, 115
MhlI GDGCHC 1 cut(s) 807
MluCI AATT 2 cut(s) 600, 755
MlyI GAGTC 3 cut(s) 173, 242, 471
MnlI CCTC 8 cut(s) 110, 259, 268, 294, 389, 454, 535, 590
MseI TTAA 3 cut(s) 53, 636, 729
MslI CAYNNNNRTG 1 cut(s) 348
MspI CCGG 2 cut(s) 65, 864
MspR9I CCNGG 6 cut(s) 65, 66, 431, 744, 852, 865
MvaI CCWGG 3 cut(s) 431, 744, 852
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 528
NciI CCSGG 3 cut(s) 65, 66, 865
NdeI CATATG 1 cut(s) 469
NdeII GATC 3 cut(s) 74, 115, 127
NlaIII CATG 5 cut(s) 91, 160, 175, 608, 717
NlaIV GGNNCC 2 cut(s) 117, 806
NmuCI GTSAC 1 cut(s) 463
NspI RCATGY 3 cut(s) 91, 160, 175
PagI TCATGA 1 cut(s) 604
PasI CCCWGGG 1 cut(s) 430
PciI ACATGT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 228, 903
PflMI CCANNNNNTGG 1 cut(s) 212
PkrI GCNGC 2 cut(s) 147, 190
PleI GAGTC 3 cut(s) 172, 242, 470
PpsI GAGTC 3 cut(s) 172, 242, 470
PscI ACATGT 1 cut(s) 156
Psp6I CCWGG 3 cut(s) 429, 742, 850
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 3 cut(s) 429, 742, 850
PspN4I GGNNCC 2 cut(s) 117, 806
PsuI RGATCY 2 cut(s) 74, 115
RseI CAYNNNNRTG 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 3 cut(s) 53, 636, 729
SatI GCNGC 2 cut(s) 146, 189
Sau3AI GATC 3 cut(s) 74, 115, 127
SchI GAGTC 3 cut(s) 173, 242, 471
ScrFI CCNGG 6 cut(s) 65, 66, 431, 744, 852, 865
SduI GDGCHC 1 cut(s) 807
SfaNI GCATC 1 cut(s) 352
SmaI CCCGGG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 348
Sse9I AATT 2 cut(s) 600, 755
SsiI CCGC 2 cut(s) 27, 146
SspMI CTAG 5 cut(s) 96, 168, 195, 245, 594
StyD4I CCNGG 6 cut(s) 63, 64, 429, 742, 850, 863
StyI CCWWGG 2 cut(s) 766, 791
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 574
TasI AATT 2 cut(s) 600, 755
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 2 cut(s) 228, 903
Tru1I TTAA 3 cut(s) 53, 636, 729
Tru9I TTAA 3 cut(s) 53, 636, 729
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 3 cut(s) 381, 593, 699
TspGWI ACGGA 3 cut(s) 167, 276, 790
TspMI CCCGGG 1 cut(s) 64
TspRI CASTG 1 cut(s) 112
Van91I CCANNNNNTGG 1 cut(s) 212
XapI RAATTY 2 cut(s) 600, 755
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 3 cut(s) 91, 160, 175
XmaI CCCGGG 1 cut(s) 64
XspI CTAG 5 cut(s) 96, 168, 195, 245, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.