Rroxscaffold_4G00304960

Myelodysplasia-myeloid leukemia factor 1-interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005437
Physical Location & Seq
Forward (+)
25602377 .. 25605684
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304960.1

Sequence Viewer

Length: 870 bp
ATGCAAAGCGAAAGGCAAGGCAGACGCGGTTTCTCTCGCTCAGATGACCTGTTAAATAGTTTCCCGGGATTTGGATCTCGTAGAAGCATGTTGCCTAGCCTCTTCAGTGGAAGGGATCCATTCGATGATCCCTTCTTCAGTCGGCCGCTTGGAGACATGTTTGAGTGTAGCATGTTCGGTCCAAGTGCTGCTTCTAGTGATACACCACAGAGTGGTAGAGATGGGGGAATCATAGTAGAAGAACTAGACTCTGATGATGATGGAGATGGAGATGAGGTTACTGGAGATGGGAGAGATAAGAGGAAGCAACGAGCTGTGTCAAGCGAAGAGCCATCTGTTGAGCATCCGGATGATGATGATATGGATGGCAAGAACACGAGTAAAAGAGGTGATTTCAACAAGGTGCAAGGCACACAGCCCCAGGGTCGTAGTGCCTCTGTTCAGAGTTGCAGAGTGAAGATTGAGGAGACCAAAGAAGCAGATAGGACAACTGGTCGAGCAACACATAAGATATCTAGAGGAATTCATGATAAGGGTCATTCTGTTACAAGGAAGCTTAACCCAGATGGAAAGGTGGATGTGTTGCAAACTCTACACAATCTGAATGAAGATGAGCTTGCTGGTTTTGAAGAAGCATGGAATGGTAATGTTAAGGTGGACTTACCTGGCTGGAAAGAATTTGACAACCCTGGACAGTGGTATGGCATTGGCGTTTCGGCAGGTGAACGGAAGGGCAATGCACCTTGGAGTGCTGGGCTCCTTCAATCTTCAGAGCAACCACAAAGCTCCAGAGGGATTAGACCAGGGAATGTAACCGGGACTACCTCTGATGGGAGAACCAAGAAGGTTGTTAGAATCAATATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
Mlf1IP

Protein Analysis

289

Amino Acids

31.62

Weight (kDa)

5.23

Isoelectric Point (pI)

54.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlf1IP PF10248 150 - 215 3.8e-12 Myelodysplasia-myeloid leukemia factor 1-interacting protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 710
Acc36I ACCTGC 1 cut(s) 710
AccB7I CCANNNNNTGG 1 cut(s) 212
AccII CGCG 1 cut(s) 27
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 2 cut(s) 27, 146
AclWI GGATC 4 cut(s) 82, 110, 122, 123
AcoI YGGCCR 1 cut(s) 143
AcsI RAATTY 2 cut(s) 522, 677
AcuI CTGAAG 3 cut(s) 88, 121, 753
AdeI CACNNNGTG 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 71, 212, 831
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 3 cut(s) 397, 629, 764
AhdI GACNNNNNGTC 1 cut(s) 492
AjnI CCWGG 4 cut(s) 420, 664, 688, 802
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 4 cut(s) 314, 556, 616, 786
AluI AGCT 4 cut(s) 314, 556, 616, 786
Alw26I GTCTC 2 cut(s) 147, 461
AlwI GGATC 4 cut(s) 82, 110, 122, 123
Ama87I CYCGRG 1 cut(s) 64
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 2 cut(s) 522, 677
AspS9I GGNCC 1 cut(s) 179
AsuC2I CCSGG 3 cut(s) 65, 66, 817
AsuHPI GGTGA 2 cut(s) 401, 734
AvaI CYCGRG 1 cut(s) 64
AvaII GGWCC 1 cut(s) 179
BamHI GGATCC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 759
BauI CACGAG 1 cut(s) 376
BbvI GCAGC 1 cut(s) 175
BccI CCATC 8 cut(s) 215, 254, 260, 281, 340, 359, 560, 824
BciT130I CCWGG 4 cut(s) 422, 666, 690, 804
BcnI CCSGG 3 cut(s) 65, 66, 817
BcoDI GTCTC 2 cut(s) 147, 461
BfaI CTAG 4 cut(s) 96, 195, 245, 516
BfuAI ACCTGC 1 cut(s) 710
BisI GCNGC 2 cut(s) 146, 189
BlsI GCNGC 2 cut(s) 147, 190
Bme1390I CCNGG 7 cut(s) 65, 66, 422, 666, 690, 804, 817
Bme18I GGWCC 1 cut(s) 179
BmeRI GACNNNNNGTC 1 cut(s) 492
BmeT110I CYCGRG 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 179
BmiI GGNNCC 2 cut(s) 117, 758
BmrFI CCNGG 7 cut(s) 65, 66, 422, 666, 690, 804, 817
BmsI GCATC 1 cut(s) 352
BpmI CTGGAG 2 cut(s) 303, 772
BpuMI CCSGG 3 cut(s) 65, 66, 817
BsaBI GATNNNNATC 1 cut(s) 73
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 6 cut(s) 64, 420, 421, 688, 743, 803
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 212, 831
Bse1I ACTGG 2 cut(s) 286, 496
Bse3DI GCAATG 1 cut(s) 742
Bse8I GATNNNNATC 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 4 cut(s) 422, 666, 690, 804
BseDI CCNNGG 6 cut(s) 64, 420, 421, 688, 743, 803
BseGI GGATG 4 cut(s) 343, 355, 370, 583
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 3 cut(s) 71, 212, 831
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 2 cut(s) 286, 496
BseRI GAGGAG 1 cut(s) 479
BseX3I CGGCCG 1 cut(s) 143
BseXI GCAGC 1 cut(s) 175
BseYI CCCAGC 1 cut(s) 752
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 1 cut(s) 146
BshFI GGCC 1 cut(s) 145
BsiEI CGRYCG 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 64
BsiSI CCGG 3 cut(s) 65, 347, 816
BslFI GGGAC 1 cut(s) 832
BslI CCNNNNNNNGG 3 cut(s) 71, 212, 831
BsmAI GTCTC 2 cut(s) 147, 461
BsmFI GGGAC 1 cut(s) 832
BsnI GGCC 1 cut(s) 145
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 64
Bsp1286I GDGCHC 1 cut(s) 759
Bsp13I TCCGGA 1 cut(s) 346
Bsp143I GATC 3 cut(s) 74, 115, 127
BspACI CCGC 2 cut(s) 27, 146
BspANI GGCC 1 cut(s) 145
BspCNI CTCAG 1 cut(s) 53
BspEI TCCGGA 1 cut(s) 346
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 117, 758
BspMI ACCTGC 1 cut(s) 710
BspPI GGATC 4 cut(s) 82, 110, 122, 123
BspQI GCTCTTC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 742
BsrI ACTGG 2 cut(s) 286, 496
BssECI CCNNGG 6 cut(s) 64, 420, 421, 688, 743, 803
BssMI GATC 3 cut(s) 74, 115, 127
BssSI CACGAG 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 743
Bst2BI CACGAG 1 cut(s) 376
Bst2UI CCWGG 4 cut(s) 422, 666, 690, 804
Bst4CI ACNGT 1 cut(s) 696
Bst6I CTCTTC 2 cut(s) 107, 321
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 1 cut(s) 40
BstF5I GGATG 4 cut(s) 343, 355, 370, 583
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 3 cut(s) 77, 118, 130
BstMAI GTCTC 2 cut(s) 147, 461
BstMBI GATC 3 cut(s) 74, 115, 127
BstMCI CGRYCG 1 cut(s) 146
BstNI CCWGG 4 cut(s) 422, 666, 690, 804
BstNSI RCATGY 3 cut(s) 91, 160, 175
BstSCI CCNGG 7 cut(s) 63, 64, 420, 664, 688, 802, 815
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 2 cut(s) 74, 115
BstYI RGATCY 2 cut(s) 74, 115
BstZI CGGCCG 1 cut(s) 143
BsuRI GGCC 1 cut(s) 145
BtsCI GGATG 4 cut(s) 343, 355, 370, 583
BtsIMutI CAGTG 2 cut(s) 112, 701
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 1 cut(s) 618
CciI TCATGA 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 179
Cfr9I CCCGGG 1 cut(s) 64
CseI GACGC 1 cut(s) 33
CviAII CATG 5 cut(s) 88, 157, 172, 527, 636
DdeI CTNAG 1 cut(s) 40
DpnI GATC 3 cut(s) 76, 117, 129
DpnII GATC 3 cut(s) 74, 115, 127
DraIII CACNNNGTG 1 cut(s) 212
DriI GACNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 143
EagI CGGCCG 1 cut(s) 143
Eam1104I CTCTTC 2 cut(s) 107, 321
Eam1105I GACNNNNNGTC 1 cut(s) 492
EarI CTCTTC 2 cut(s) 107, 321
EclXI CGGCCG 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 759
Eco31I GGTCTC 1 cut(s) 461
Eco32I GATATC 1 cut(s) 513
Eco47I GGWCC 1 cut(s) 179
Eco52I CGGCCG 1 cut(s) 143
Eco57I CTGAAG 3 cut(s) 88, 121, 753
Eco88I CYCGRG 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 522
EcoRII CCWGG 4 cut(s) 420, 664, 688, 802
EcoRV GATATC 1 cut(s) 513
EcoT14I CCWWGG 1 cut(s) 743
EcoT38I GRGCYC 1 cut(s) 759
ErhI CCWWGG 1 cut(s) 743
FaeI CATG 5 cut(s) 91, 160, 175, 530, 639
FalI AAGNNNNNCTT 6 cut(s) 175, 207, 600, 632, 644, 676
FaqI GGGAC 1 cut(s) 832
FatI CATG 5 cut(s) 87, 156, 171, 526, 635
Fnu4HI GCNGC 2 cut(s) 146, 189
FokI GGATG 4 cut(s) 330, 362, 377, 590
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 2 cut(s) 146, 189
FspBI CTAG 4 cut(s) 96, 195, 245, 516
GluI GCNGC 2 cut(s) 146, 189
GsaI CCCAGC 1 cut(s) 756
GsuI CTGGAG 2 cut(s) 303, 772
HaeIII GGCC 1 cut(s) 145
HapII CCGG 3 cut(s) 65, 347, 816
HgaI GACGC 1 cut(s) 33
Hin1II CATG 5 cut(s) 91, 160, 175, 530, 639
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 3 cut(s) 228, 248, 855
HpaII CCGG 3 cut(s) 65, 347, 816
HphI GGTGA 2 cut(s) 401, 734
Hpy166II GTNNAC 2 cut(s) 658, 725
Hpy188I TCNGA 6 cut(s) 43, 253, 444, 603, 772, 829
Hpy188III TCNNGA 4 cut(s) 347, 516, 527, 789
Hpy8I GTNNAC 2 cut(s) 658, 725
HpyAV CCTTC 5 cut(s) 105, 142, 724, 770, 838
HpyCH4III ACNGT 1 cut(s) 696
HpyCH4V TGCA 5 cut(s) 4, 406, 450, 586, 740
HpyF3I CTNAG 1 cut(s) 40
Hsp92II CATG 5 cut(s) 91, 160, 175, 530, 639
Kpn2I TCCGGA 1 cut(s) 346
Kzo9I GATC 3 cut(s) 74, 115, 127
LguI GCTCTTC 1 cut(s) 321
LmnI GCTCC 2 cut(s) 762, 791
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 1 cut(s) 352
MaeI CTAG 4 cut(s) 96, 195, 245, 516
MaeIII GTNAC 3 cut(s) 277, 544, 811
MalI GATC 3 cut(s) 76, 117, 129
MboI GATC 3 cut(s) 74, 115, 127
MboII GAAGA 8 cut(s) 94, 127, 251, 338, 469, 620, 641, 759
MflI RGATCY 2 cut(s) 74, 115
MhlI GDGCHC 1 cut(s) 759
MluCI AATT 2 cut(s) 522, 677
MlyI GAGTC 1 cut(s) 242
MnlI CCTC 9 cut(s) 110, 268, 294, 380, 445, 457, 512, 785, 835
MroI TCCGGA 1 cut(s) 346
MseI TTAA 3 cut(s) 53, 558, 651
MslI CAYNNNNRTG 1 cut(s) 348
MspI CCGG 3 cut(s) 65, 347, 816
MspR9I CCNGG 7 cut(s) 65, 66, 422, 666, 690, 804, 817
MvaI CCWGG 4 cut(s) 422, 666, 690, 804
MvnI CGCG 1 cut(s) 27
NciI CCSGG 3 cut(s) 65, 66, 817
NdeII GATC 3 cut(s) 74, 115, 127
NlaIII CATG 5 cut(s) 91, 160, 175, 530, 639
NlaIV GGNNCC 2 cut(s) 117, 758
NspI RCATGY 3 cut(s) 91, 160, 175
PagI TCATGA 1 cut(s) 526
PaqCI CACCTGC 1 cut(s) 710
PasI CCCWGGG 1 cut(s) 421
PciI ACATGT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 228, 855
PflMI CCANNNNNTGG 1 cut(s) 212
PkrI GCNGC 2 cut(s) 147, 190
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PscI ACATGT 1 cut(s) 156
Psp6I CCWGG 4 cut(s) 420, 664, 688, 802
PspFI CCCAGC 1 cut(s) 752
PspGI CCWGG 4 cut(s) 420, 664, 688, 802
PspN4I GGNNCC 2 cut(s) 117, 758
PspPI GGNCC 1 cut(s) 179
PsuI RGATCY 2 cut(s) 74, 115
RseI CAYNNNNRTG 1 cut(s) 348
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 3 cut(s) 53, 558, 651
SatI GCNGC 2 cut(s) 146, 189
Sau3AI GATC 3 cut(s) 74, 115, 127
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 7 cut(s) 65, 66, 422, 666, 690, 804, 817
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 1 cut(s) 352
SinI GGWCC 1 cut(s) 179
SmaI CCCGGG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 348
Sse9I AATT 2 cut(s) 522, 677
SsiI CCGC 2 cut(s) 27, 146
SspMI CTAG 4 cut(s) 96, 195, 245, 516
StyD4I CCNGG 7 cut(s) 63, 64, 420, 664, 688, 802, 815
StyI CCWWGG 1 cut(s) 743
TaaI ACNGT 1 cut(s) 696
TaqI TCGA 2 cut(s) 123, 496
TaqII GACCGA 1 cut(s) 167
TasI AATT 2 cut(s) 522, 677
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 2 cut(s) 228, 855
Tru1I TTAA 3 cut(s) 53, 558, 651
Tru9I TTAA 3 cut(s) 53, 558, 651
TscAI CASTG 2 cut(s) 112, 701
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 2 cut(s) 515, 621
TspGWI ACGGA 1 cut(s) 742
TspMI CCCGGG 1 cut(s) 64
TspRI CASTG 2 cut(s) 112, 701
Van91I CCANNNNNTGG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 179
XapI RAATTY 2 cut(s) 522, 677
XbaI TCTAGA 1 cut(s) 515
XceI RCATGY 3 cut(s) 91, 160, 175
XmaI CCCGGG 1 cut(s) 64
XspI CTAG 4 cut(s) 96, 195, 245, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.