MD02G1211600.v1.1

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008059
Physical Location & Seq
Forward (+)
22607113 .. 22607418
306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1211600.v1.1.491

Sequence Viewer

Length: 306 bp
ATGATGAAGGGCATTGTGATCTCAATGTTGGTTGTGCTAGCCATGGCTCATCTAATGGTGCAGCAAGGAGAGGCCATTGTCAATTGTGGCCAAGTGGACTCGAACTTGGCACCTTGCATTTCCTACCTGTCCAAACGGGGTAGTCTTGACCCTGCTTGCTGCGCTGGGGTGCAAAATATCAAGAACCTCGCCCAAACCACAGCTGATAAGCAAGCCGCTTGCGAATGCTGTATGGCAGCGAGAACCGCTTCCCCAATGTTAACGAAGTTACAGCTTCTACTCTCCCAGGCAAGTGTGGGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

102

Amino Acids

10.56

Weight (kDa)

8.21

Isoelectric Point (pI)

41.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 29 - 85 9.3e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AciI CCGC 2 cut(s) 216, 246
AcoI YGGCCR 1 cut(s) 88
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 2 cut(s) 203, 274
AluI AGCT 2 cut(s) 203, 274
AoxI GGCC 2 cut(s) 72, 88
ApeKI GCWGC 3 cut(s) 61, 159, 236
Asp700I GAANNNNTTC 1 cut(s) 247
AspLEI GCGC 1 cut(s) 164
AsuNHI GCTAGC 1 cut(s) 37
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 109
BbvI GCAGC 3 cut(s) 73, 146, 248
BciT130I CCWGG 1 cut(s) 287
BfaI CTAG 1 cut(s) 38
BisI GCNGC 4 cut(s) 62, 160, 216, 237
BlsI GCNGC 4 cut(s) 63, 161, 217, 238
Bme1390I CCNGG 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 287
BmtI GCTAGC 1 cut(s) 41
BsaJI CCNNGG 2 cut(s) 42, 285
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 42, 285
BseXI GCAGC 3 cut(s) 73, 146, 248
BseYI CCCAGC 1 cut(s) 164
BsgI GTGCAG 1 cut(s) 80
BshFI GGCC 2 cut(s) 74, 90
BshNI GGYRCC 1 cut(s) 109
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 2 cut(s) 74, 90
Bsp143I GATC 1 cut(s) 18
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 2 cut(s) 216, 246
BspANI GGCC 2 cut(s) 74, 90
BspLI GGNNCC 1 cut(s) 111
BspOI GCTAGC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 109
BssECI CCNNGG 2 cut(s) 42, 285
BssMI GATC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 287
BstC8I GCNNGC 4 cut(s) 39, 157, 213, 220
BstDSI CCRYGG 1 cut(s) 42
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 2 cut(s) 161, 245
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstV1I GCAGC 3 cut(s) 73, 146, 248
BsuRI GGCC 2 cut(s) 74, 90
BtgI CCRYGG 1 cut(s) 42
Cac8I GCNNGC 4 cut(s) 39, 157, 213, 220
CfoI GCGC 1 cut(s) 164
CviAII CATG 1 cut(s) 43
CviJI RGCY 7 cut(s) 41, 47, 74, 90, 203, 215, 274
CviKI_1 RGCY 7 cut(s) 41, 47, 74, 90, 203, 215, 274
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 46
FaiI YATR 2 cut(s) 44, 233
FatI CATG 1 cut(s) 42
Fnu4HI GCNGC 4 cut(s) 62, 160, 216, 237
Fsp4HI GCNGC 4 cut(s) 62, 160, 216, 237
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 163
GluI GCNGC 4 cut(s) 62, 160, 216, 237
GsaI CCCAGC 1 cut(s) 168
HaeIII GGCC 2 cut(s) 74, 90
HhaI GCGC 1 cut(s) 164
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HinfI GANTC 1 cut(s) 98
HpaI GTTAAC 1 cut(s) 261
Hpy166II GTNNAC 2 cut(s) 97, 261
Hpy188III TCNNGA 2 cut(s) 146, 181
Hpy8I GTNNAC 2 cut(s) 97, 261
HpyCH4V TGCA 3 cut(s) 61, 117, 172
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 245
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 162
KspAI GTTAAC 1 cut(s) 261
Kzo9I GATC 1 cut(s) 18
LpnPI CCDG 5 cut(s) 140, 150, 165, 272, 299
Lsp1109I GCAGC 3 cut(s) 73, 146, 248
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 267
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MfeI CAATTG 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 1 cut(s) 82
MluNI TGGCCA 1 cut(s) 90
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 2 cut(s) 64, 197
Mox20I TGGCCA 1 cut(s) 90
MroXI GAANNNNTTC 1 cut(s) 247
MscI TGGCCA 1 cut(s) 90
MseI TTAA 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 287
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 2 cut(s) 161, 245
NcoI CCATGG 1 cut(s) 42
NdeII GATC 1 cut(s) 18
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 1 cut(s) 111
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 247
PkrI GCNGC 4 cut(s) 63, 161, 217, 238
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 285
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 203
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 4 cut(s) 62, 160, 216, 237
Sau3AI GATC 1 cut(s) 18
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 287
SetI ASST 5 cut(s) 115, 129, 189, 205, 276
Sse9I AATT 1 cut(s) 82
SsiI CCGC 2 cut(s) 216, 246
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 1 cut(s) 42
TaqI TCGA 1 cut(s) 101
TasI AATT 1 cut(s) 82
TauI GCSGC 1 cut(s) 218
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TseI GCWGC 3 cut(s) 61, 159, 236
TspDTI ATGAA 1 cut(s) 20
XmnI GAANNNNTTC 1 cut(s) 247
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.