Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008059

Physical Location & Seq

Genomic Coordinates Forward (+)

11263341 .. 11263688

Gene Structure 348 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1144900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 348 bp

ATGAAGCGCGTTGTTGTAGCGGCAGTGGCAGTGGTGTTGGCCATGTTGGTCTTGGTTGAACCGGGGCATGCCACGGTCACTTGCCAACAAGCAGTGTCATCTCTGACTCCTTGCCTTCCATATCTCACCAGCGGCGCTGAAACCCCGCCTGCGGCGTGTTGCAGTGGGGTTTCGGGTCTGAAACTGCTCACTCAAACCACCGAAGATAGGCGTACAGCCTGCCAATGCCTTAAGGACGCGGCAAGTCAGAACCAAGGCATTAGGGAAGACGCTGCTGCTGGTCTCCCTGCGTCGTGTCAAGTTCAAATCAACGTTCCCATTTCTAGGAGCGTTGACTGCGACCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Tryp_alpha_amyl

Protein Analysis

116

Amino Acids

11.87

Weight (kDa)

5.65

Isoelectric Point (pI)

48.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LTP_2	PF14368	10 - 104	1.7e-09	Probable lipid transfer
Tryp_alpha_amyl	PF00234	28 - 113	2.1e-13	Protease inhibitor/seed storage/LTP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008059)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59330
fragaria_vesca	FvH4_7g10020
malus_domestica	MD02G1211600.v1.1 MD07G1105600.v1.1 MD09G1144900.v1.1 MD17G1131400.v1.1
prunus_persica	Prupe.2G129200_v2.0.a1 Prupe.3G183000_v2.0.a1
pyrus_communis	pycom02g17710 pycom07g09010 pycom07g09080 pycom09g06430 pycom17g12420
rosa_chinensis	RchiOBHm_Chr1g0347601
rosa_laevigata	RLG00000028734
rosa_multiflora	Rmu_sc0006589.1_g000010 Rmu_sc0010621.1_g000005
rosa_roxburghii	Rroxscaffold_4G00307610
rosa_rugosa	Rorug01G0188200
rosa_samantha	Rh1AG205100 Rh1BG171000 Rh1CG190400 Rh1DG201200
rosa_wichuraiana	Rw0G018180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	9, 239
AciI	CCGC	5 cut(s)	20, 132, 146, 152, 239
AclI	AACGTT	1 cut(s)	312
AcoI	YGGCCR	1 cut(s)	39
AfaI	GTAC	1 cut(s)	214
AfiI	CCNNNNNNNGG	3 cut(s)	151, 207, 324
AflII	CTTAAG	1 cut(s)	230
AgsI	TTSAA	2 cut(s)	59, 305
Alw26I	GTCTC	1 cut(s)	287
AoxI	GGCC	1 cut(s)	39
ApeKI	GCWGC	2 cut(s)	272, 275
AspLEI	GCGC	2 cut(s)	9, 137
AsuC2I	CCSGG	1 cut(s)	63
AsuHPI	GGTGA	1 cut(s)	118
BalI	TGGCCA	1 cut(s)	41
BbsI	GAAGAC	1 cut(s)	273
BbvI	GCAGC	2 cut(s)	259, 262
BcnI	CCSGG	1 cut(s)	63
BcoDI	GTCTC	1 cut(s)	287
BfaI	CTAG	1 cut(s)	324
BfoI	RGCGCY	1 cut(s)	138
BfrI	CTTAAG	1 cut(s)	230
BisI	GCNGC	6 cut(s)	21, 133, 153, 240, 273, 276
BlsI	GCNGC	6 cut(s)	22, 134, 154, 241, 274, 277
Bme1390I	CCNGG	1 cut(s)	63
BmrFI	CCNGG	1 cut(s)	63
BpiI	GAAGAC	1 cut(s)	273
BpuMI	CCSGG	1 cut(s)	63
BsaI	GGTCTC	1 cut(s)	287
BsaJI	CCNNGG	3 cut(s)	62, 72, 253
Bsc4I	CCNNNNNNNGG	3 cut(s)	151, 207, 324
Bse1I	ACTGG	1 cut(s)	343
BseDI	CCNNGG	3 cut(s)	62, 72, 253
BseLI	CCNNNNNNNGG	3 cut(s)	151, 207, 324
BseNI	ACTGG	1 cut(s)	343
BseXI	GCAGC	2 cut(s)	259, 262
Bsh1236I	CGCG	2 cut(s)	9, 239
BshFI	GGCC	1 cut(s)	41
BsiSI	CCGG	1 cut(s)	62
BslI	CCNNNNNNNGG	3 cut(s)	151, 207, 324
BsmAI	GTCTC	1 cut(s)	287
BsnI	GGCC	1 cut(s)	41
Bso31I	GGTCTC	1 cut(s)	287
BspACI	CCGC	5 cut(s)	20, 132, 146, 152, 239
BspANI	GGCC	1 cut(s)	41
BspFNI	CGCG	2 cut(s)	9, 239
BspTI	CTTAAG	1 cut(s)	230
BspTNI	GGTCTC	1 cut(s)	287
BsrI	ACTGG	1 cut(s)	343
BssECI	CCNNGG	3 cut(s)	62, 72, 253
BssT1I	CCWWGG	1 cut(s)	253
Bst4CI	ACNGT	1 cut(s)	76
BstAFI	CTTAAG	1 cut(s)	230
BstC8I	GCNNGC	3 cut(s)	69, 150, 220
BstDSI	CCRYGG	1 cut(s)	72
BstFNI	CGCG	2 cut(s)	9, 239
BstH2I	RGCGCY	1 cut(s)	138
BstHHI	GCGC	2 cut(s)	9, 137
BstMAI	GTCTC	1 cut(s)	287
BstMWI	GCNNNNNNNGC	2 cut(s)	26, 336
BstNSI	RCATGY	1 cut(s)	71
BstSCI	CCNGG	1 cut(s)	61
BstUI	CGCG	2 cut(s)	9, 239
BstV1I	GCAGC	2 cut(s)	259, 262
BstV2I	GAAGAC	1 cut(s)	273
BsuRI	GGCC	1 cut(s)	41
BtgI	CCRYGG	1 cut(s)	72
BtsI	GCAGTG	4 cut(s)	30, 36, 99, 169
BtsIMutI	CAGTG	4 cut(s)	30, 36, 99, 169
Cac8I	GCNNGC	3 cut(s)	69, 150, 220
CfoI	GCGC	2 cut(s)	9, 137
CseI	GACGC	3 cut(s)	245, 278, 279
Csp6I	GTAC	1 cut(s)	213
CviAII	CATG	2 cut(s)	43, 68
CviJI	RGCY	2 cut(s)	41, 218
CviKI_1	RGCY	2 cut(s)	41, 218
CviQI	GTAC	1 cut(s)	213
EaeI	YGGCCR	1 cut(s)	39
Eco130I	CCWWGG	1 cut(s)	253
Eco31I	GGTCTC	1 cut(s)	287
EcoT14I	CCWWGG	1 cut(s)	253
ErhI	CCWWGG	1 cut(s)	253
FaeI	CATG	2 cut(s)	46, 71
FaiI	YATR	3 cut(s)	44, 69, 121
FatI	CATG	2 cut(s)	42, 67
FauI	CCCGC	1 cut(s)	153
Fnu4HI	GCNGC	6 cut(s)	21, 133, 153, 240, 273, 276
Fsp4HI	GCNGC	6 cut(s)	21, 133, 153, 240, 273, 276
FspBI	CTAG	1 cut(s)	324
GlaI	GCGC	2 cut(s)	8, 136
GluI	GCNGC	6 cut(s)	21, 133, 153, 240, 273, 276
HaeII	RGCGCY	1 cut(s)	138
HaeIII	GGCC	1 cut(s)	41
HapII	CCGG	1 cut(s)	62
HgaI	GACGC	3 cut(s)	245, 278, 279
HhaI	GCGC	2 cut(s)	9, 137
Hin1II	CATG	2 cut(s)	46, 71
Hin6I	GCGC	2 cut(s)	7, 135
HinP1I	GCGC	2 cut(s)	7, 135
HincII	GTYRAC	1 cut(s)	334
HindII	GTYRAC	1 cut(s)	334
HinfI	GANTC	1 cut(s)	106
HpaII	CCGG	1 cut(s)	62
HphI	GGTGA	1 cut(s)	118
Hpy166II	GTNNAC	1 cut(s)	334
Hpy188I	TCNGA	3 cut(s)	105, 180, 249
Hpy8I	GTNNAC	1 cut(s)	334
Hpy99I	CGWCG	1 cut(s)	295
HpyAV	CCTTC	1 cut(s)	125
HpyCH4III	ACNGT	1 cut(s)	76
HpyCH4IV	ACGT	1 cut(s)	312
HpyCH4V	TGCA	1 cut(s)	162
HpyF10VI	GCNNNNNNNGC	2 cut(s)	26, 336
HpySE526I	ACGT	1 cut(s)	312
Hsp92II	CATG	2 cut(s)	46, 71
HspAI	GCGC	2 cut(s)	7, 135
LmnI	GCTCC	1 cut(s)	327
LpnPI	CCDG	6 cut(s)	75, 142, 162, 232, 264, 300
Lsp1109I	GCAGC	2 cut(s)	259, 262
MaeI	CTAG	1 cut(s)	324
MaeII	ACGT	1 cut(s)	312
MaeIII	GTNAC	1 cut(s)	76
MboII	GAAGA	2 cut(s)	215, 278
MlsI	TGGCCA	1 cut(s)	41
MluNI	TGGCCA	1 cut(s)	41
MlyI	GAGTC	1 cut(s)	100
Mox20I	TGGCCA	1 cut(s)	41
MscI	TGGCCA	1 cut(s)	41
MseI	TTAA	1 cut(s)	231
Msp20I	TGGCCA	1 cut(s)	41
MspA1I	CMGCKG	1 cut(s)	132
MspCI	CTTAAG	1 cut(s)	230
MspI	CCGG	1 cut(s)	62
MspR9I	CCNGG	1 cut(s)	63
MvnI	CGCG	2 cut(s)	9, 239
MwoI	GCNNNNNNNGC	2 cut(s)	26, 336
NciI	CCSGG	1 cut(s)	63
NlaIII	CATG	2 cut(s)	46, 71
NmuCI	GTSAC	1 cut(s)	76
NspI	RCATGY	1 cut(s)	71
PaeI	GCATGC	1 cut(s)	71
PkrI	GCNGC	6 cut(s)	22, 134, 154, 241, 274, 277
PleI	GAGTC	1 cut(s)	100
PpsI	GAGTC	1 cut(s)	100
Psp1406I	AACGTT	1 cut(s)	312
RsaI	GTAC	1 cut(s)	214
RsaNI	GTAC	1 cut(s)	213
SaqAI	TTAA	1 cut(s)	231
SatI	GCNGC	6 cut(s)	21, 133, 153, 240, 273, 276
SchI	GAGTC	1 cut(s)	100
ScrFI	CCNGG	1 cut(s)	63
SetI	ASST	1 cut(s)	315
SmlI	CTYRAG	1 cut(s)	230
SmoI	CTYRAG	1 cut(s)	230
SphI	GCATGC	1 cut(s)	71
SsiI	CCGC	5 cut(s)	20, 132, 146, 152, 239
SspMI	CTAG	1 cut(s)	324
StyD4I	CCNGG	1 cut(s)	61
StyI	CCWWGG	1 cut(s)	253
TaaI	ACNGT	1 cut(s)	76
TaiI	ACGT	1 cut(s)	315
TauI	GCSGC	4 cut(s)	23, 135, 155, 242
Tru1I	TTAA	1 cut(s)	231
Tru9I	TTAA	1 cut(s)	231
TscAI	CASTG	4 cut(s)	30, 36, 99, 169
TseFI	GTSAC	1 cut(s)	76
TseI	GCWGC	2 cut(s)	272, 275
Tsp45I	GTSAC	1 cut(s)	76
TspDTI	ATGAA	1 cut(s)	17
TspRI	CASTG	4 cut(s)	30, 36, 99, 169
Vha464I	CTTAAG	1 cut(s)	230
XceI	RCATGY	1 cut(s)	71
XcmI	CCANNNNNNNNNTGG	1 cut(s)	49
XspI	CTAG	1 cut(s)	324

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.