MD17G1131400.v1.1

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008059
Physical Location & Seq
Forward (+)
11587232 .. 11587579
348 bp
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UTR
Exon/CDS
Intron
MD17G1131400.v1.1.491

Sequence Viewer

Length: 348 bp
ATGAAGGGCGTCGTTATAGCGGCAGTGGTGGTGGTGCTGGCCATGTTGGTCTTGGTTGAACCGGGGCATGCCACGGTCACTTGCCAACAAGCAATGTCATCTGTGACTCCTTGCCTTCAATATCTTACCAGCGGCTCTGGCACCCCTCCTGTGGCATGTTGCAGTGGTGTTTCGGGTCTGAGACAGCTCACTCAAACCACCGAAGATAGGCGTACAGCCTGCCAATGCCTCATGGACGCAGCGAATCAAAACCAAGACATTAAGGAAGCTGCTGCGGCTGGCCTTCCCGCGGCATGCCGAGTTCAGATCAACGTTCCAATTTCTAGGAGCGTCGACTGCAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

116

Amino Acids

11.9

Weight (kDa)

7.51

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 10 - 104 2.3e-09 Probable lipid transfer
Tryp_alpha_amyl PF00234 28 - 113 1.1e-11 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 290
AciI CCGC 5 cut(s) 20, 132, 275, 288, 290
AclI AACGTT 1 cut(s) 312
AcoI YGGCCR 1 cut(s) 39
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 3 cut(s) 151, 207, 289
AgsI TTSAA 2 cut(s) 59, 119
AluBI AGCT 2 cut(s) 187, 269
AluI AGCT 2 cut(s) 187, 269
Alw26I GTCTC 1 cut(s) 175
AoxI GGCC 2 cut(s) 39, 280
ApeKI GCWGC 3 cut(s) 239, 269, 272
AsuC2I CCSGG 1 cut(s) 63
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 140
BbvI GCAGC 3 cut(s) 251, 256, 259
BcnI CCSGG 1 cut(s) 63
BcoDI GTCTC 1 cut(s) 175
BfaI CTAG 1 cut(s) 324
BisI GCNGC 7 cut(s) 21, 133, 240, 270, 273, 276, 291
BlsI GCNGC 7 cut(s) 22, 134, 241, 271, 274, 277, 292
Bme1390I CCNGG 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 62, 72, 288
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 207, 289
Bse3DI GCAATG 1 cut(s) 99
BseDI CCNNGG 3 cut(s) 62, 72, 288
BseLI CCNNNNNNNGG 3 cut(s) 151, 207, 289
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 170
BseXI GCAGC 3 cut(s) 251, 256, 259
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 2 cut(s) 41, 282
BshNI GGYRCC 1 cut(s) 140
BsiSI CCGG 1 cut(s) 62
BslI CCNNNNNNNGG 3 cut(s) 151, 207, 289
BsmAI GTCTC 1 cut(s) 175
BsnI GGCC 2 cut(s) 41, 282
Bsp143I GATC 1 cut(s) 306
BspACI CCGC 5 cut(s) 20, 132, 275, 288, 290
BspANI GGCC 2 cut(s) 41, 282
BspCNI CTCAG 1 cut(s) 171
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 99
BssECI CCNNGG 3 cut(s) 62, 72, 288
BssMI GATC 1 cut(s) 306
BssNI GRCGYC 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 76
BstACI GRCGYC 1 cut(s) 9
BstC8I GCNNGC 5 cut(s) 39, 69, 220, 280, 295
BstDEI CTNAG 1 cut(s) 179
BstDSI CCRYGG 2 cut(s) 72, 288
BstFNI CGCG 1 cut(s) 290
BstKTI GATC 1 cut(s) 309
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 1 cut(s) 306
BstMWI GCNNNNNNNGC 3 cut(s) 138, 275, 336
BstNSI RCATGY 3 cut(s) 71, 159, 297
BstSCI CCNGG 1 cut(s) 61
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 3 cut(s) 251, 256, 259
BsuRI GGCC 2 cut(s) 41, 282
BtgI CCRYGG 2 cut(s) 72, 288
BtsI GCAGTG 2 cut(s) 30, 169
BtsIMutI CAGTG 2 cut(s) 30, 169
Cac8I GCNNGC 5 cut(s) 39, 69, 220, 280, 295
Cfr42I CCGCGG 1 cut(s) 291
CseI GACGC 2 cut(s) 245, 319
Csp6I GTAC 1 cut(s) 213
CviAII CATG 5 cut(s) 43, 68, 156, 232, 294
CviJI RGCY 7 cut(s) 41, 135, 187, 218, 269, 278, 282
CviKI_1 RGCY 7 cut(s) 41, 135, 187, 218, 269, 278, 282
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 1 cut(s) 179
DpnI GATC 1 cut(s) 308
DpnII GATC 1 cut(s) 306
EaeI YGGCCR 1 cut(s) 39
FaeI CATG 5 cut(s) 46, 71, 159, 235, 297
FaiI YATR 6 cut(s) 17, 44, 69, 157, 233, 295
FatI CATG 5 cut(s) 42, 67, 155, 231, 293
FauI CCCGC 1 cut(s) 295
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 7 cut(s) 21, 133, 240, 270, 273, 276, 291
Fsp4HI GCNGC 7 cut(s) 21, 133, 240, 270, 273, 276, 291
FspBI CTAG 1 cut(s) 324
GluI GCNGC 7 cut(s) 21, 133, 240, 270, 273, 276, 291
HaeIII GGCC 2 cut(s) 41, 282
HapII CCGG 1 cut(s) 62
HgaI GACGC 2 cut(s) 245, 319
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 5 cut(s) 46, 71, 159, 235, 297
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HinfI GANTC 2 cut(s) 106, 244
HpaII CCGG 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 2 cut(s) 180, 306
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 2 cut(s) 14, 335
HpyAV CCTTC 2 cut(s) 125, 293
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 2 cut(s) 162, 339
HpyF10VI GCNNNNNNNGC 3 cut(s) 138, 275, 336
HpyF3I CTNAG 1 cut(s) 179
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 5 cut(s) 46, 71, 159, 235, 297
KspI CCGCGG 1 cut(s) 291
Kzo9I GATC 1 cut(s) 306
LmnI GCTCC 1 cut(s) 327
LpnPI CCDG 7 cut(s) 23, 75, 123, 142, 162, 232, 264
Lsp1109I GCAGC 3 cut(s) 251, 256, 259
MaeI CTAG 1 cut(s) 324
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 76, 103
MalI GATC 1 cut(s) 308
MboI GATC 1 cut(s) 306
MboII GAAGA 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 1 cut(s) 318
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 100
MnlI CCTC 2 cut(s) 156, 239
Mox20I TGGCCA 1 cut(s) 41
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 261
Msp20I TGGCCA 1 cut(s) 41
MspA1I CMGCKG 2 cut(s) 132, 290
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 138, 275, 336
NciI CCSGG 1 cut(s) 63
NdeII GATC 1 cut(s) 306
NlaIII CATG 5 cut(s) 46, 71, 159, 235, 297
NlaIV GGNNCC 1 cut(s) 142
NmeAIII GCCGAG 1 cut(s) 323
NmuCI GTSAC 2 cut(s) 76, 103
NspI RCATGY 3 cut(s) 71, 159, 297
PaeI GCATGC 2 cut(s) 71, 297
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 7 cut(s) 22, 134, 241, 271, 274, 277, 292
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
Psp1406I AACGTT 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 142
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SacII CCGCGG 1 cut(s) 291
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 7 cut(s) 21, 133, 240, 270, 273, 276, 291
Sau3AI GATC 1 cut(s) 306
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 1 cut(s) 63
SetI ASST 3 cut(s) 189, 271, 315
Sfr303I CCGCGG 1 cut(s) 291
SgrBI CCGCGG 1 cut(s) 291
SphI GCATGC 2 cut(s) 71, 297
Sse9I AATT 1 cut(s) 318
SsiI CCGC 5 cut(s) 20, 132, 275, 288, 290
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 61
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 1 cut(s) 315
TaqI TCGA 1 cut(s) 333
TasI AATT 1 cut(s) 318
TauI GCSGC 4 cut(s) 23, 135, 278, 293
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 30, 169
TseFI GTSAC 2 cut(s) 76, 103
TseI GCWGC 3 cut(s) 239, 269, 272
Tsp45I GTSAC 2 cut(s) 76, 103
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 30, 169
XceI RCATGY 3 cut(s) 71, 159, 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.