pycom02g17710

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008059
Physical Location & Seq
Forward (+)
15972003 .. 15972464
462 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g17710.1

Sequence Viewer

Length: 354 bp
ATGATGAAGGGCATTGTGATCTCAATGTTGGTTGTGCTAGCCATGGCTCATCTAATGGTACAGCAAGGAGAGGCCATTGTCAATTGTGGCCAAGTGGACTCAAACTTGGCACCTTGCGTTTCCTACCTGTCCAAACGGGGTAGTCTTGACCCTGCTTGCTGCGCCGGGGTGCAGAACATCAAGAACCTCGCCCAAACCACAGCTGATAAGCAGGCTGCCTGTGAATGCTGTAAGGCAGCGGCGAACCGCTTCCCCAATGTTGACGAAGTTACAGCTTCTACTCTCCCAGGCAAGTGTGGGATTGCGATGAATATCCCCATCTCAAAGACCACCAACTGCCAGAGCATAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2 Tryp_alpha_amyl

Protein Analysis

118

Amino Acids

12.22

Weight (kDa)

8.09

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 29 - 113 6.8e-11 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 109
AciI CCGC 2 cut(s) 239, 247
AcoI YGGCCR 1 cut(s) 88
AfaI GTAC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 2 cut(s) 203, 275
AluI AGCT 2 cut(s) 203, 275
AoxI GGCC 2 cut(s) 72, 88
ApeKI GCWGC 3 cut(s) 159, 215, 236
Asp700I GAANNNNTTC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 166
AsuNHI GCTAGC 1 cut(s) 37
BalI TGGCCA 1 cut(s) 90
BanI GGYRCC 1 cut(s) 109
BbvI GCAGC 3 cut(s) 146, 202, 248
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 1 cut(s) 288
BcnI CCSGG 1 cut(s) 166
BfaI CTAG 1 cut(s) 38
BisI GCNGC 4 cut(s) 160, 216, 237, 240
BlsI GCNGC 4 cut(s) 161, 217, 238, 241
Bme1390I CCNGG 2 cut(s) 166, 288
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 2 cut(s) 166, 288
BmtI GCTAGC 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 311
BsaJI CCNNGG 3 cut(s) 42, 165, 286
Bse8I GATNNNNATC 1 cut(s) 311
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 3 cut(s) 42, 165, 286
BseJI GATNNNNATC 1 cut(s) 311
BseXI GCAGC 3 cut(s) 146, 202, 248
BsgI GTGCAG 1 cut(s) 191
BshFI GGCC 2 cut(s) 74, 90
BshNI GGYRCC 1 cut(s) 109
BsiSI CCGG 1 cut(s) 165
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 2 cut(s) 74, 90
Bsp143I GATC 1 cut(s) 18
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 2 cut(s) 239, 247
BspANI GGCC 2 cut(s) 74, 90
BspLI GGNNCC 1 cut(s) 111
BspOI GCTAGC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 109
BssECI CCNNGG 3 cut(s) 42, 165, 286
BssMI GATC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 288
BstC8I GCNNGC 3 cut(s) 39, 157, 213
BstDSI CCRYGG 1 cut(s) 42
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 2 cut(s) 164, 286
BstV1I GCAGC 3 cut(s) 146, 202, 248
BsuRI GGCC 2 cut(s) 74, 90
BtgI CCRYGG 1 cut(s) 42
BtgZI GCGATG 1 cut(s) 320
Cac8I GCNNGC 3 cut(s) 39, 157, 213
CfoI GCGC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 59
CviAII CATG 1 cut(s) 43
CviJI RGCY 7 cut(s) 41, 47, 74, 90, 203, 215, 275
CviKI_1 RGCY 7 cut(s) 41, 47, 74, 90, 203, 215, 275
CviQI GTAC 1 cut(s) 59
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 46
FaiI YATR 2 cut(s) 44, 347
FatI CATG 1 cut(s) 42
Fnu4HI GCNGC 4 cut(s) 160, 216, 237, 240
Fsp4HI GCNGC 4 cut(s) 160, 216, 237, 240
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 163
GluI GCNGC 4 cut(s) 160, 216, 237, 240
HaeIII GGCC 2 cut(s) 74, 90
HapII CCGG 1 cut(s) 165
HhaI GCGC 1 cut(s) 164
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 1 cut(s) 98
HpaII CCGG 1 cut(s) 165
Hpy166II GTNNAC 2 cut(s) 97, 262
Hpy188III TCNNGA 2 cut(s) 146, 181
Hpy8I GTNNAC 2 cut(s) 97, 262
HpyCH4V TGCA 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 162
Kzo9I GATC 1 cut(s) 18
LpnPI CCDG 7 cut(s) 140, 165, 178, 197, 232, 273, 300
Lsp1109I GCAGC 3 cut(s) 146, 202, 248
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 268
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MfeI CAATTG 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 90
MluCI AATT 1 cut(s) 82
MluNI TGGCCA 1 cut(s) 90
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 2 cut(s) 64, 197
Mox20I TGGCCA 1 cut(s) 90
MroXI GAANNNNTTC 1 cut(s) 248
MscI TGGCCA 1 cut(s) 90
Msp20I TGGCCA 1 cut(s) 90
MspA1I CMGCKG 2 cut(s) 203, 239
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 2 cut(s) 166, 288
MunI CAATTG 1 cut(s) 82
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 161
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 42
NdeII GATC 1 cut(s) 18
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 1 cut(s) 111
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 248
PkrI GCNGC 4 cut(s) 161, 217, 238, 241
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
SatI GCNGC 4 cut(s) 160, 216, 237, 240
Sau3AI GATC 1 cut(s) 18
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 2 cut(s) 166, 288
SetI ASST 5 cut(s) 115, 129, 189, 205, 277
Sse9I AATT 1 cut(s) 82
SsiI CCGC 2 cut(s) 239, 247
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 164, 286
StyI CCWWGG 1 cut(s) 42
TasI AATT 1 cut(s) 82
TauI GCSGC 1 cut(s) 242
TseI GCWGC 3 cut(s) 159, 215, 236
TspDTI ATGAA 2 cut(s) 20, 323
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.