Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005468

Physical Location & Seq

Genomic Coordinates Reverse (-)

25399601 .. 25404447

Gene Structure 4847 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1218600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGCCGCCAATCCCATTTCTCATAGACGAGCATTGGCACTCCTTAGACAGCAATTCTCCAGCCCTACCCTCAACTCTCAGCGAGCTTCTCTTCTCAGGTGCCGTTCCTCTCAGAAAACCCAGAACCACAAAGACGATGAACAACTGCAGAACAATACCCAACGACCCAAATTGTCAACCCCCAAAACCAAAAGGGCTAAGGATATGGCCAAGCTTATCGACTCCATGCCGTGGTCAACTGAGCTCGAATCTTCTCTCTCTACAATCGCTTCCTCTCTCTCCAAAACGACAGTGCACCAAACTCTCCACCTTATCAAAGCCCCCAGCAAAGCCCTTCAATTTTTCAAGTGGGCGGAGGTAATGGGTTTCTCTCACAATGACCAATCCTACTTCTTGATGCTTGAAATCTTGGGTCGCAATCGAAATCTCAATGCTGCTCGAAATTTCTTGTTTTCGATTGAAAAGAAGTCTAATGGGGCAGTTAAGCTCGAGGACCGGTTCTTTAATAGCTTGATAAGGAACTACGGTCGAGCTGGGCTGTTCCAAGAATCTATAAAGCTTTTTAGTACAATGAAGTCGATTGGTGTTTCGCCTTCTGTTGTTTCGTTCAATAGTCTGCTGTTGATTTTGCTTAGAAAGGGGCGGACCAATATGGCCAAGAATGTGTATGATGAAATGGTTAGTATGTATGGAGCTACACCAGATACTTGTACGTTTAATACTTTGATTAGAGGGTTTTGTATGAACTCCATGGTTGATGAAGGTTTTCGCTTTTTCAAAGAAATGTCGCGTTTCAAATGTGATCCAGATGTCATTACCTATAATACTCTTGTTGATGGATTGTGTAGAGCAGGGAAGGTTGGTATTGCCCATAATGTGGTGAAGGGCATGAGTAAGAGAAGTGCAGATTTGAATCCAAATATTGTTACTTATACGACCTTGATTAGAGGTTATTGTATGAAACAAGAGATTGATGAGGCTTTATGTGTTTTGGAGGAGATGACTAGCCAAGGGTTGAAACCAAATGGAATTACCTGCAATACTCTAATTAAGGGGCTTTGTGAAGCGCATAAGCTAGATAAGATAAAAGACATTTTGGAAGGAACAATGAGCGGTGGAGAATTTACTCCTGATACTTGTACGTTTAACACACTGATGCATTCGCATTGCAATGCAGGAAACTTGGATGAAGCATTGAAGGTTTTTGCAAAGATGTCAGAGCTAAAGGTCCCACCAGATTCAGCTACATACAGTGTTTTGATACGCAGTTTGTGTCAACGAGGGGATTACAGTAGAGCAGAGGAGTTGTTCGATGAGCTATCAAAGAAAGAAATACTGTTAAGTGATGACGGTTGTAAGCCTCTTGTTGCCTCTTATAACCCTATTTTTGAGTATTTGTGTAGTAAGGGGAAAATTAAAAAGGCCGAGGCAGTGTTTAGGCAGCTAATGAGAAGAGGAACGCAAGACCCTGTGTCCTATAAGACTTTGATCATGGGCCATTGTAAGGAAGGTACATTTGAAACCGGGTATGAACTCCTAGTCTGGATGCTTAGAAGGGATTTTGTGCCTGATGTCGAAATTTATGAATCCTTGATTGGTGGTCTCTTGCAGAAGGGTAAGGCCCTTCTTGCTCAACAGACGCTAGAGAAGATGCTGAAAAGCTCTCATCTCCCAAAAACGTGTACATTCCATTGTATACTAGCAGAACTTTTAGAAAAGAATTGTGCCCTAGAATCTGCCAATTGTGTTATTTTAATGCTGGAGAGAAAAATTAGACAAAATATTAATCTCTCAACACATCTTGTAAGGCTACTTTTCAGCAGTGGGTTGCGTGACAAAGCATTTGACATTGTAGGGATGCTCCATAAGAATGGATACTCAATTAAAATGGAAGAAGTGGTTCATTTTCTTTGCCAGAGAAGAAAGCTGTTAGACGCATGCAAAATGTTGCAATTCAGTCTGCAGAAGCATCAAAGTGTCAGTATTGATATTTTCAACCAAGTCATCGAAGGGCTTTGTAATATCAATAAACCTTCAGAAGCATTTGGCTTGTACTATGAACTGGTTGAGAATGCAGGCTATCAGCAATTTCCCTGCTTAGGATCCTTAAAATCTGCACTAGAGATTGCAGGAAAATCAGTTGAAGCTGAGTTTCTATCTAAAGGGATGCCAGGAGAGCAGTCGCTAGACAAATCCCATAGGTCGAGACCTCAACTGAGCATGCTGAACAGAATGAATGTTATTGTTCTCAATATATGGAATGGGCCATCACATGGTTGTATAGTCTTGTGGAGTTTCCAGAAAAATTCCTTCGCATCGAAAGTTGTGTTCTTGGCATGGTACACGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Chal_sti_synt_C Chal_sti_synt_N PPR PPR_1 PPR_2 PPR_3

Protein Analysis

784

Amino Acids

88.31

Weight (kDa)

9.09

Isoelectric Point (pI)

41.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	168 - 207	6.2e-07	PPR repeat family
PPR_2	PF13041	198 - 247	9.9e-12	PPR repeat family
PPR_1	PF12854	231 - 263	1.4e-10	PPR repeat
PPR_2	PF13041	234 - 283	2.3e-17	PPR repeat family
PPR	PF01535	238 - 267	1.2e-06	PPR repeat
PPR_long	PF17177	248 - 367	2.8e-09	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	259 - 316	2.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	267 - 297	3.3e-11	PPR repeat
PPR_2	PF13041	269 - 303	2.6e-06	PPR repeat family
TPR_24	PF23276	283 - 404	9e-10	Fungal tetratrico peptide repeats
PPR_3	PF13812	302 - 351	1.6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	304 - 335	5.2e-10	PPR repeat
PPR_2	PF13041	306 - 354	4.8e-17	PPR repeat family
PPR	PF01535	309 - 339	9.6e-07	PPR repeat
PPR_3	PF13812	330 - 387	9.7e-08	Pentatricopeptide repeat domain
PPR_3	PF13812	374 - 422	8.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	376 - 406	8.4e-11	PPR repeat
PPR_2	PF13041	377 - 426	4.2e-17	PPR repeat family
PPR	PF01535	381 - 408	1.2e-06	PPR repeat
PPR_long	PF17177	393 - 486	3.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	410 - 439	2e-07	PPR repeat
PPR	PF01535	416 - 443	1e-05	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005468)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02060
fragaria_vesca	FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470
malus_domestica	MD02G1218600.v1.1
prunus_persica	Prupe.2G122500_v2.0.a1
pyrus_communis	pycom12652g00050
rosa_chinensis	RchiOBHm_Chr1g0346171
rosa_laevigata	RLG00000028810
rosa_multiflora	Rmu_sc0003809.1_g000024
rosa_roxburghii	Rroxscaffold_4G00308800
rosa_rugosa	Rorug01G0178600
rosa_samantha	Rh1AG197700 Rh1BG162900 Rh1BG163000 Rh1BG163800 Rh1CG182800 Rh1DG193600 Rh1DG193700 Rh1DG194800
rosa_wichuraiana	Rw1G016310 Rw1G016590 Rw1G016620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1377
Acc36I	ACCTGC	1 cut(s)	1043
AccB1I	GGYRCC	1 cut(s)	99
AccB7I	CCANNNNNTGG	3 cut(s)	231, 877, 2273
AccBSI	CCGCTC	1 cut(s)	1113
AccI	GTMKAC	1 cut(s)	1696
AccII	CGCG	1 cut(s)	790
AciI	CCGC	4 cut(s)	6, 353, 643, 1113
AclWI	GGATC	3 cut(s)	797, 2097, 2110
AcoI	YGGCCR	3 cut(s)	3, 207, 654
AcsI	RAATTY	4 cut(s)	442, 1121, 1578, 2305
AcuI	CTGAAG	1 cut(s)	2019
AfaI	GTAC	7 cut(s)	568, 712, 1141, 1513, 1684, 2054, 2342
AfiI	CCNNNNNNNGG	5 cut(s)	231, 877, 1504, 2099, 2273
AflIII	ACRYGT	1 cut(s)	1679
AgeI	ACCGGT	1 cut(s)	495
AhdI	GACNNNNNGTC	1 cut(s)	1471
AjnI	CCWGG	1 cut(s)	2170
AjuI	GAANNNNNNNTTGG	2 cut(s)	1578, 1610
Alw21I	GWGCWC	2 cut(s)	246, 297
Alw26I	GTCTC	2 cut(s)	1607, 2200
Alw44I	GTGCAC	1 cut(s)	293
AlwI	GGATC	3 cut(s)	797, 2097, 2110
Ama87I	CYCGRG	1 cut(s)	488
AoxI	GGCC	7 cut(s)	3, 207, 654, 1422, 1495, 1620, 2264
ApaLI	GTGCAC	1 cut(s)	293
ApeKI	GCWGC	2 cut(s)	434, 1441
ApoI	RAATTY	4 cut(s)	442, 1121, 1578, 2305
ArsI	GACNNNNNNTTYG	2 cut(s)	1826, 1858
AseI	ATTAAT	1 cut(s)	1785
AsiGI	ACCGGT	1 cut(s)	495
Asp700I	GAANNNNTTC	2 cut(s)	765, 1899
AspLEI	GCGC	1 cut(s)	1069
AspS9I	GGNCC	6 cut(s)	493, 645, 1228, 1495, 1621, 2264
AsuC2I	CCSGG	1 cut(s)	1525
AsuHPI	GGTGA	1 cut(s)	892
AvaI	CYCGRG	1 cut(s)	488
AvaII	GGWCC	3 cut(s)	493, 645, 1228
BaeGI	GKGCMC	2 cut(s)	297, 1729
BaeI	ACNNNNGTAYC	1 cut(s)	2332
BalI	TGGCCA	2 cut(s)	209, 656
BamHI	GGATCC	1 cut(s)	2102
BanI	GGYRCC	1 cut(s)	99
BanII	GRGCYC	1 cut(s)	246
BauI	CACGAG	1 cut(s)	2344
Bbv12I	GWGCWC	2 cut(s)	246, 297
BbvI	GCAGC	2 cut(s)	421, 1453
BccI	CCATC	2 cut(s)	830, 2275
BceAI	ACGGC	2 cut(s)	87, 214
BciT130I	CCWGG	1 cut(s)	2172
BciVI	GTATCC	1 cut(s)	1868
BclI	TGATCA	1 cut(s)	1488
BcnI	CCSGG	1 cut(s)	1525
BcoDI	GTCTC	2 cut(s)	1607, 2200
BfaI	CTAG	8 cut(s)	1005, 1076, 1538, 1643, 1700, 1730, 2120, 2186
BfmI	CTRYAG	2 cut(s)	146, 1961
BfuAI	ACCTGC	1 cut(s)	1043
BfuI	GTATCC	1 cut(s)	1868
BisI	GCNGC	3 cut(s)	6, 435, 1442
BlsI	GCNGC	3 cut(s)	7, 436, 1443
Bme1390I	CCNGG	2 cut(s)	1525, 2172
Bme18I	GGWCC	3 cut(s)	493, 645, 1228
BmeRI	GACNNNNNGTC	1 cut(s)	1471
BmeT110I	CYCGRG	1 cut(s)	488
BmgT120I	GGNCC	6 cut(s)	493, 645, 1228, 1495, 1621, 2264
BmiI	GGNNCC	3 cut(s)	101, 1230, 2104
BmrFI	CCNGG	2 cut(s)	1525, 2172
BmsI	GCATC	8 cut(s)	387, 1146, 1536, 1641, 1848, 1978, 2157, 2324
BpmI	CTGGAG	2 cut(s)	43, 1781
Bpu10I	CCTNAGC	2 cut(s)	198, 2098
BpuMI	CCSGG	1 cut(s)	1525
BsaBI	GATNNNNATC	1 cut(s)	912
BsaI	GGTCTC	2 cut(s)	1607, 2200
BsaJI	CCNNGG	4 cut(s)	230, 750, 1009, 1425
BsaWI	WCCGGW	1 cut(s)	495
Bsc4I	CCNNNNNNNGG	5 cut(s)	231, 877, 1504, 2099, 2273
Bse118I	RCCGGY	1 cut(s)	495
Bse1I	ACTGG	1 cut(s)	2067
Bse3DI	GCAATG	2 cut(s)	1165, 1177
Bse8I	GATNNNNATC	1 cut(s)	912
BseBI	CCWGG	1 cut(s)	2172
BseDI	CCNNGG	4 cut(s)	230, 750, 1009, 1425
BseGI	GGATG	4 cut(s)	1192, 1551, 1863, 2172
BseJI	GATNNNNATC	1 cut(s)	912
BseLI	CCNNNNNNNGG	5 cut(s)	231, 877, 1504, 2099, 2273
BseMI	GCAATG	2 cut(s)	1165, 1177
BseMII	CTCAG	6 cut(s)	92, 109, 125, 231, 2139, 2207
BseNI	ACTGG	1 cut(s)	2067
BseRI	GAGGAG	2 cut(s)	1010, 1316
BseSI	GKGCMC	2 cut(s)	297, 1729
BseXI	GCAGC	2 cut(s)	421, 1453
BseYI	CCCAGC	2 cut(s)	323, 533
BsgI	GTGCAG	2 cut(s)	924, 2100
Bsh1236I	CGCG	1 cut(s)	790
Bsh1285I	CGRYCG	1 cut(s)	529
BshFI	GGCC	7 cut(s)	5, 209, 656, 1424, 1497, 1622, 2266
BshNI	GGYRCC	1 cut(s)	99
BshTI	ACCGGT	1 cut(s)	495
BsiEI	CGRYCG	1 cut(s)	529
BsiHKAI	GWGCWC	2 cut(s)	246, 297
BsiHKCI	CYCGRG	1 cut(s)	488
BsiSI	CCGG	2 cut(s)	496, 1524
BslFI	GGGAC	1 cut(s)	1214
BslI	CCNNNNNNNGG	5 cut(s)	231, 877, 1504, 2099, 2273
BsmAI	GTCTC	2 cut(s)	1607, 2200
BsmFI	GGGAC	1 cut(s)	1214
BsmI	GAATGC	2 cut(s)	1159, 2077
BsnI	GGCC	7 cut(s)	5, 209, 656, 1424, 1497, 1622, 2266
Bso31I	GGTCTC	2 cut(s)	1607, 2200
BsoBI	CYCGRG	1 cut(s)	488
Bsp1286I	GDGCHC	3 cut(s)	246, 297, 1729
Bsp1407I	TGTACA	1 cut(s)	1682
Bsp143I	GATC	3 cut(s)	802, 1488, 2102
Bsp19I	CCATGG	1 cut(s)	750
BspACI	CCGC	4 cut(s)	6, 353, 643, 1113
BspANI	GGCC	7 cut(s)	5, 209, 656, 1424, 1497, 1622, 2266
BspCNI	CTCAG	6 cut(s)	91, 108, 124, 232, 2140, 2208
BspFNI	CGCG	1 cut(s)	790
BspLI	GGNNCC	3 cut(s)	101, 1230, 2104
BspMAI	CTGCAG	2 cut(s)	150, 1965
BspMI	ACCTGC	1 cut(s)	1043
BspPI	GGATC	3 cut(s)	797, 2097, 2110
BspT107I	GGYRCC	1 cut(s)	99
BspTNI	GGTCTC	2 cut(s)	1607, 2200
BsrBI	CCGCTC	1 cut(s)	1113
BsrDI	GCAATG	2 cut(s)	1165, 1177
BsrFI	RCCGGY	1 cut(s)	495
BsrGI	TGTACA	1 cut(s)	1682
BsrI	ACTGG	1 cut(s)	2067
BssAI	RCCGGY	1 cut(s)	495
BssECI	CCNNGG	4 cut(s)	230, 750, 1009, 1425
BssMI	GATC	3 cut(s)	802, 1488, 2102
BssNAI	GTATAC	1 cut(s)	1697
BssSI	CACGAG	1 cut(s)	2344
BssT1I	CCWWGG	2 cut(s)	750, 1009
Bst1107I	GTATAC	1 cut(s)	1697
Bst2BI	CACGAG	1 cut(s)	2344
Bst2UI	CCWGG	1 cut(s)	2172
Bst4CI	ACNGT	6 cut(s)	292, 527, 1253, 1292, 1338, 1352
Bst6I	CTCTTC	2 cut(s)	96, 1447
BstAUI	TGTACA	1 cut(s)	1682
BstC8I	GCNNGC	4 cut(s)	84, 1939, 2077, 2222
BstDSI	CCRYGG	2 cut(s)	230, 750
BstF5I	GGATG	4 cut(s)	1192, 1551, 1863, 2172
BstFNI	CGCG	1 cut(s)	790
BstHHI	GCGC	1 cut(s)	1069
BstKTI	GATC	3 cut(s)	805, 1491, 2105
BstMAI	GTCTC	2 cut(s)	1607, 2200
BstMBI	GATC	3 cut(s)	802, 1488, 2102
BstMCI	CGRYCG	1 cut(s)	529
BstMWI	GCNNNNNNNGC	2 cut(s)	1628, 2176
BstNI	CCWGG	1 cut(s)	2172
BstNSI	RCATGY	2 cut(s)	1941, 2224
BstSCI	CCNGG	2 cut(s)	1523, 2170
BstSFI	CTRYAG	2 cut(s)	146, 1961
BstSLI	GKGCMC	2 cut(s)	297, 1729
BstUI	CGCG	1 cut(s)	790
BstV1I	GCAGC	2 cut(s)	421, 1453
BstX2I	RGATCY	1 cut(s)	2102
BstXI	CCANNNNNNTGG	1 cut(s)	1871
BstYI	RGATCY	1 cut(s)	2102
BstZ17I	GTATAC	1 cut(s)	1697
BsuI	GTATCC	1 cut(s)	1868
BsuRI	GGCC	7 cut(s)	5, 209, 656, 1424, 1497, 1622, 2266
BtgI	CCRYGG	2 cut(s)	230, 750
BtsCI	GGATG	4 cut(s)	1192, 1551, 1863, 2172
BtsI	GCAGTG	2 cut(s)	1437, 1828
BtsIMutI	CAGTG	5 cut(s)	297, 1151, 1258, 1437, 1828
BveI	ACCTGC	1 cut(s)	1043
Cac8I	GCNNGC	4 cut(s)	84, 1939, 2077, 2222
CfoI	GCGC	1 cut(s)	1069
Cfr10I	RCCGGY	1 cut(s)	495
Cfr13I	GGNCC	6 cut(s)	493, 645, 1228, 1495, 1621, 2264
CseI	GACGC	2 cut(s)	1648, 1943
Csp6I	GTAC	7 cut(s)	567, 711, 1140, 1512, 1683, 2053, 2341
CspAI	ACCGGT	1 cut(s)	495
CviAII	CATG	8 cut(s)	226, 751, 889, 1492, 1938, 2221, 2273, 2337
CviQI	GTAC	7 cut(s)	567, 711, 1140, 1512, 1683, 2053, 2341
DpnI	GATC	3 cut(s)	804, 1490, 2104
DpnII	GATC	3 cut(s)	802, 1488, 2102
DriI	GACNNNNNGTC	1 cut(s)	1471
EaeI	YGGCCR	3 cut(s)	3, 207, 654
Eam1104I	CTCTTC	2 cut(s)	96, 1447
Eam1105I	GACNNNNNGTC	1 cut(s)	1471
EarI	CTCTTC	2 cut(s)	96, 1447
EciI	GGCGGA	2 cut(s)	368, 658
Ecl136II	GAGCTC	1 cut(s)	244
Eco130I	CCWWGG	2 cut(s)	750, 1009
Eco24I	GRGCYC	1 cut(s)	246
Eco31I	GGTCTC	2 cut(s)	1607, 2200
Eco47I	GGWCC	3 cut(s)	493, 645, 1228
Eco53kI	GAGCTC	1 cut(s)	244
Eco57I	CTGAAG	1 cut(s)	2019
Eco88I	CYCGRG	1 cut(s)	488
EcoICRI	GAGCTC	1 cut(s)	244
EcoO109I	RGGNCCY	2 cut(s)	1228, 1621
EcoRII	CCWGG	1 cut(s)	2170
EcoT14I	CCWWGG	2 cut(s)	750, 1009
EcoT22I	ATGCAT	1 cut(s)	1161
EcoT38I	GRGCYC	1 cut(s)	246
ErhI	CCWWGG	2 cut(s)	750, 1009
FaeI	CATG	8 cut(s)	229, 754, 892, 1495, 1941, 2224, 2276, 2340
FaqI	GGGAC	1 cut(s)	1214
FatI	CATG	8 cut(s)	225, 750, 888, 1491, 1937, 2220, 2272, 2336
FbaI	TGATCA	1 cut(s)	1488
FblI	GTMKAC	1 cut(s)	1696
Fnu4HI	GCNGC	3 cut(s)	6, 435, 1442
FokI	GGATG	4 cut(s)	1199, 1558, 1870, 2179
FriOI	GRGCYC	1 cut(s)	246
Fsp4HI	GCNGC	3 cut(s)	6, 435, 1442
FspBI	CTAG	8 cut(s)	1005, 1076, 1538, 1643, 1700, 1730, 2120, 2186
GlaI	GCGC	1 cut(s)	1068
GluI	GCNGC	3 cut(s)	6, 435, 1442
GsaI	CCCAGC	2 cut(s)	327, 537
GsuI	CTGGAG	2 cut(s)	43, 1781
HaeIII	GGCC	7 cut(s)	5, 209, 656, 1424, 1497, 1622, 2266
HapII	CCGG	2 cut(s)	496, 1524
HgaI	GACGC	2 cut(s)	1648, 1943
HhaI	GCGC	1 cut(s)	1069
Hin1II	CATG	8 cut(s)	229, 754, 892, 1495, 1941, 2224, 2276, 2340
Hin6I	GCGC	1 cut(s)	1067
HinP1I	GCGC	1 cut(s)	1067
HincII	GTYRAC	3 cut(s)	177, 237, 1277
HindII	GTYRAC	3 cut(s)	177, 237, 1277
HindIII	AAGCTT	2 cut(s)	212, 557
HinfI	GANTC	7 cut(s)	221, 248, 548, 913, 1238, 1586, 1733
HpaII	CCGG	2 cut(s)	496, 1524
HphI	GGTGA	1 cut(s)	892
Hpy166II	GTNNAC	7 cut(s)	177, 237, 295, 1277, 1683, 1697, 2343
Hpy188I	TCNGA	3 cut(s)	114, 1219, 2038
Hpy188III	TCNNGA	6 cut(s)	394, 806, 1130, 1543, 2205, 2299
Hpy8I	GTNNAC	7 cut(s)	177, 237, 295, 1277, 1683, 1697, 2343
HpyCH4III	ACNGT	6 cut(s)	292, 527, 1253, 1292, 1338, 1352
HpyCH4IV	ACGT	3 cut(s)	713, 1142, 1679
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1628, 2176
HpySE526I	ACGT	3 cut(s)	713, 1142, 1679
Hsp92II	CATG	8 cut(s)	229, 754, 892, 1495, 1941, 2224, 2276, 2340
HspAI	GCGC	1 cut(s)	1067
Ksp22I	TGATCA	1 cut(s)	1488
Kzo9I	GATC	3 cut(s)	802, 1488, 2102
LmnI	GCTCC	2 cut(s)	692, 1866
Lsp1109I	GCAGC	2 cut(s)	421, 1453
LweI	GCATC	8 cut(s)	387, 1146, 1536, 1641, 1848, 1978, 2157, 2324
MaeI	CTAG	8 cut(s)	1005, 1076, 1538, 1643, 1700, 1730, 2120, 2186
MaeII	ACGT	3 cut(s)	713, 1142, 1679
MaeIII	GTNAC	2 cut(s)	925, 1832
MalI	GATC	3 cut(s)	804, 1490, 2104
MbiI	CCGCTC	1 cut(s)	1113
MboI	GATC	3 cut(s)	802, 1488, 2102
MboII	GAAGA	6 cut(s)	83, 243, 1464, 1660, 1904, 1932
MfeI	CAATTG	1 cut(s)	1741
MflI	RGATCY	1 cut(s)	2102
MhlI	GDGCHC	3 cut(s)	246, 297, 1729
MlsI	TGGCCA	2 cut(s)	209, 656
MluNI	TGGCCA	2 cut(s)	209, 656
MlyI	GAGTC	1 cut(s)	215
Mox20I	TGGCCA	2 cut(s)	209, 656
Mph1103I	ATGCAT	1 cut(s)	1161
MroXI	GAANNNNTTC	2 cut(s)	765, 1899
MscI	TGGCCA	2 cut(s)	209, 656
MslI	CAYNNNNRTG	4 cut(s)	1154, 1170, 1869, 1974
Msp20I	TGGCCA	2 cut(s)	209, 656
MspI	CCGG	2 cut(s)	496, 1524
MspR9I	CCNGG	2 cut(s)	1525, 2172
MunI	CAATTG	1 cut(s)	1741
Mva1269I	GAATGC	2 cut(s)	1159, 2077
MvaI	CCWGG	1 cut(s)	2172
MvnI	CGCG	1 cut(s)	790
MwoI	GCNNNNNNNGC	2 cut(s)	1628, 2176
NciI	CCSGG	1 cut(s)	1525
NcoI	CCATGG	1 cut(s)	750
NdeII	GATC	3 cut(s)	802, 1488, 2102
NlaIII	CATG	8 cut(s)	229, 754, 892, 1495, 1941, 2224, 2276, 2340
NlaIV	GGNNCC	3 cut(s)	101, 1230, 2104
NmeAIII	GCCGAG	1 cut(s)	1450
NmuCI	GTSAC	1 cut(s)	1832
NsiI	ATGCAT	1 cut(s)	1161
NspI	RCATGY	2 cut(s)	1941, 2224
PaeI	GCATGC	2 cut(s)	1941, 2224
PaeR7I	CTCGAG	1 cut(s)	488
PctI	GAATGC	2 cut(s)	1159, 2077
PdmI	GAANNNNTTC	2 cut(s)	765, 1899
PfeI	GAWTC	6 cut(s)	248, 548, 913, 1238, 1586, 1733
PflMI	CCANNNNNTGG	3 cut(s)	231, 877, 2273
PinAI	ACCGGT	1 cut(s)	495
PkrI	GCNGC	3 cut(s)	7, 436, 1443
PleI	GAGTC	1 cut(s)	215
PpsI	GAGTC	1 cut(s)	215
PpuMI	RGGWCCY	1 cut(s)	1228
PshBI	ATTAAT	1 cut(s)	1785
PsiI	TTATAA	1 cut(s)	1377
Psp124BI	GAGCTC	1 cut(s)	246
Psp5II	RGGWCCY	1 cut(s)	1228
Psp6I	CCWGG	1 cut(s)	2170
PspFI	CCCAGC	2 cut(s)	323, 533
PspGI	CCWGG	1 cut(s)	2170
PspN4I	GGNNCC	3 cut(s)	101, 1230, 2104
PspPI	GGNCC	6 cut(s)	493, 645, 1228, 1495, 1621, 2264
PspPPI	RGGWCCY	1 cut(s)	1228
PspXI	VCTCGAGB	1 cut(s)	488
PstI	CTGCAG	2 cut(s)	150, 1965
PsuI	RGATCY	1 cut(s)	2102
RsaI	GTAC	7 cut(s)	568, 712, 1141, 1513, 1684, 2054, 2342
RsaNI	GTAC	7 cut(s)	567, 711, 1140, 1512, 1683, 2053, 2341
RseI	CAYNNNNRTG	4 cut(s)	1154, 1170, 1869, 1974
SacI	GAGCTC	1 cut(s)	246
SatI	GCNGC	3 cut(s)	6, 435, 1442
Sau3AI	GATC	3 cut(s)	802, 1488, 2102
Sau96I	GGNCC	6 cut(s)	493, 645, 1228, 1495, 1621, 2264
SchI	GAGTC	1 cut(s)	215
ScrFI	CCNGG	2 cut(s)	1525, 2172
SduI	GDGCHC	3 cut(s)	246, 297, 1729
SfaNI	GCATC	8 cut(s)	387, 1146, 1536, 1641, 1848, 1978, 2157, 2324
SfcI	CTRYAG	2 cut(s)	146, 1961
Sfr274I	CTCGAG	1 cut(s)	488
SinI	GGWCC	3 cut(s)	493, 645, 1228
SlaI	CTCGAG	1 cut(s)	488
SmiMI	CAYNNNNRTG	4 cut(s)	1154, 1170, 1869, 1974
SmlI	CTYRAG	1 cut(s)	488
SmoI	CTYRAG	1 cut(s)	488
SphI	GCATGC	2 cut(s)	1941, 2224
SsiI	CCGC	4 cut(s)	6, 353, 643, 1113
SspI	AATATT	2 cut(s)	922, 1783
SspMI	CTAG	8 cut(s)	1005, 1076, 1538, 1643, 1700, 1730, 2120, 2186
SstI	GAGCTC	1 cut(s)	246
StyD4I	CCNGG	2 cut(s)	1523, 2170
StyI	CCWWGG	2 cut(s)	750, 1009
TaaI	ACNGT	6 cut(s)	292, 527, 1253, 1292, 1338, 1352
TaiI	ACGT	3 cut(s)	716, 1145, 1682
TatI	WGTACW	3 cut(s)	566, 1682, 2052
TauI	GCSGC	1 cut(s)	8
TfiI	GAWTC	6 cut(s)	248, 548, 913, 1238, 1586, 1733
TscAI	CASTG	5 cut(s)	297, 1158, 1258, 1437, 1828
TseFI	GTSAC	1 cut(s)	1832
TseI	GCWGC	2 cut(s)	434, 1441
Tsp45I	GTSAC	1 cut(s)	1832
TspRI	CASTG	5 cut(s)	297, 1158, 1258, 1437, 1828
Van91I	CCANNNNNTGG	3 cut(s)	231, 877, 2273
VneI	GTGCAC	1 cut(s)	293
VpaK11BI	GGWCC	3 cut(s)	493, 645, 1228
VspI	ATTAAT	1 cut(s)	1785
XapI	RAATTY	4 cut(s)	442, 1121, 1578, 2305
XceI	RCATGY	2 cut(s)	1941, 2224
XhoI	CTCGAG	1 cut(s)	488
XmiI	GTMKAC	1 cut(s)	1696
XmnI	GAANNNNTTC	2 cut(s)	765, 1899
XspI	CTAG	8 cut(s)	1005, 1076, 1538, 1643, 1700, 1730, 2120, 2186
Zsp2I	ATGCAT	1 cut(s)	1161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.