Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0005468

Physical Location & Seq

Genomic Coordinates Reverse (-)

36296470 .. 36299572

Gene Structure 3103 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G016310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2037 bp

ATGGCGTTGCTCATCAACTCCAACCCATGGTCATCTCGCCTCCAGTCTTCTCTCTCTTCTCTCTCTCCCTCCATCTCCACCACCACCGTCTCCCAAACCCTCCGCCACATCAAAACCCCAACCCAAGCCCTCAAATTCTTCAACTGGGCCGAATCAATGGGCTTCTCCCACAACGCCCAATCCTTCTTCATGTTGCTTGAGATTTTCGGCCGGAACCGGAATCTCAATGCCGCTAGAAATCTCTTGTTTTCAATTGAGAAGAGGTCCAATGGGAAGGTTAAGCTCGAGGATAGGTTTTTTAATAGCTTGATTAGGAGCTATGGGGAAGCAGGGTTGTTCCAGGAGTCTGTAAAGCTTTTTGGGACGATGAAAACGATGGGGATATCGGCTTCTGTTGTTAGTTTCAATAGTTTGCTTTCGATTTTGCTTAAGAGGGGTAGGACTAGTATGGCTAAGAATGTTTATGATGAAATGCTTGGGATGTATGGAGTTACGCCCGATACGCATACGTTTAATATTTTGATTAGGGGGTTCTGTATGAACTCCATGGTTGATGAGGGGTTTCATTATTTCAAGGAAATGTCAAGGTTGAAATGTGAGCCGGATGTGGTTACTTATAATACGATTGTGGATGGGTTGTGTAGAGCAGGGAAGTTTGAGATTGCGCGAAATGTGGTGAAGGGGATGAGGAAGAGAAGTGGGGAGTTGAATCCGAATGTTGTTACTTATACGACATTGATTAGAGGGTATTGTGTGAAAGAAGATGTTGATGAGGCTTTGTGTGTTTTGGAGGAGATGACTAGTCAAGGGTTGAAACCAAATGAAATTACCTATAATACTCTGCTTAAAGGGCTTTGTGAAGCAAAGAAGCTAGATAAGGTGAAAGAAGTCTTGGGGGGAACAATGAGAGGTGAAGGATTTACTCCTGATACGTGCACGTTCAACACGCTCATGCATTCGCATTGCAATGCAGGAAACTTAGAGGAAGCATTGAAGGTTTTCACAATGATGTCAGAGTTACAGGTCCCTCCGGATTCAGCTACATACAGCGTTTTGATACGCAGTTTGTGTCAGCGAAGAGATTATGGTAAAGCTGAGGAGTTGTTTGATGAGCTATCCAAGAAACAAATCTTGTTAAGTGATCGTGGCTGTACACCTGTTGTTGCCTCATACAAACCTATCTTTGAGTACTTATGTAGTAATGGGAGAACTAAGAAAGCAGATGAAGTGTTTAGGCAGCTACTGAAAAGAGGAACACAAGATCCTCCATCCTACAAGACTTTGATCATGGGGCACTGTAGGGAAGGCACATTTGAAGCTGGCTACAAACTCCTAGTCTTTATGCTTAGAAGGGATTTTGTTCCTGATGTTGAGATTTATGATTCCTTGATTGATGGTCTGTTGGAAAAGGGTAATCCCCTTCTTGCACAACAGACTCTAGAGAAGATGCTGAAGAGCTCTCATCTCCCAAAAACATCTACTTTCCATTCTATACTGGCCGCTCTTTTAGAAATGCATTGTGCCCGTGAATCTGCAAGCTTTATTATTTTAATGTTGGAGAAAAACATTAGACAAAATATTAACCTCTCAACAGATCTTATAAGGCTTCTTTTCAGTGAGGGATTGCGAGATAAAGCATTTGAGGTTGTAGGGATGCTGCATGATAACGGATACTCGATTAAAATAGAAGAAGTGGTCAGTTTCCTTTACCAGAGCAGAAAGCTAATAGAGGCATGTGAAATATTGCGATTTAGTCTGCAGAAGCAAGAAAATGTCAGTATTGATATATTTGACCAAGTTATATTAGGGCTATGTGATATAAATAAGCTTCGAGAAGCATTTGGCTTGTACTATGAACTGATTGAGAATGGCGACCACCAGCAGTTGCCGTGCTTACATCATTTAAAGTCTTCCCTAGAGGTTGCAGGTAGATCAGTTGAAGCTGACTTTGTCTCTAAAAGAATGCCAAAACATCAGCTGGTGGACAAATGTGGGAAGTCAAGACCTCAACTAAGACCATGCTGTACAGAGTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Chal_sti_synt_C Chal_sti_synt_N PPR PPR_1 PPR_2 PPR_3

Protein Analysis

678

Amino Acids

76.78

Weight (kDa)

8.44

Isoelectric Point (pI)

45.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	131 - 179	6.8e-13	PPR repeat family
PPR_long	PF17177	151 - 299	6.5e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	163 - 195	3.6e-08	PPR repeat
PPR_2	PF13041	166 - 215	3.3e-16	PPR repeat family
PPR	PF01535	170 - 199	7.8e-06	PPR repeat
PPR_1	PF12854	199 - 230	1.8e-11	PPR repeat
PPR_2	PF13041	201 - 234	2e-07	PPR repeat family
TPR_24	PF23276	215 - 297	2e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	236 - 267	5e-11	PPR repeat
PPR_3	PF13812	236 - 283	6.8e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	238 - 286	1.5e-19	PPR repeat family
PPR	PF01535	241 - 271	1.1e-07	PPR repeat
PPR_1	PF12854	270 - 293	1.5e-07	PPR repeat
PPR_3	PF13812	302 - 354	1.2e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	306 - 337	1.3e-10	PPR repeat
PPR_2	PF13041	309 - 358	1.8e-16	PPR repeat family
PPR	PF01535	313 - 340	2.8e-06	PPR repeat
PPR_1	PF12854	342 - 371	5.4e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005468)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02060
fragaria_vesca	FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470
malus_domestica	MD02G1218600.v1.1
prunus_persica	Prupe.2G122500_v2.0.a1
pyrus_communis	pycom12652g00050
rosa_chinensis	RchiOBHm_Chr1g0346171
rosa_laevigata	RLG00000028810
rosa_multiflora	Rmu_sc0003809.1_g000024
rosa_roxburghii	Rroxscaffold_4G00308800
rosa_rugosa	Rorug01G0178600
rosa_samantha	Rh1AG197700 Rh1BG162900 Rh1BG163000 Rh1BG163800 Rh1CG182800 Rh1DG193600 Rh1DG193700 Rh1DG194800
rosa_wichuraiana	Rw1G016310 Rw1G016590 Rw1G016620

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	618, 1601
Acc36I	ACCTGC	1 cut(s)	1916
AccB7I	CCANNNNNTGG	1 cut(s)	27
AccBSI	CCGCTC	1 cut(s)	1502
AccII	CGCG	1 cut(s)	667
AccIII	TCCGGA	1 cut(s)	1030
AciI	CCGC	3 cut(s)	103, 231, 1500
AclWI	GGATC	1 cut(s)	1256
AcoI	YGGCCR	2 cut(s)	208, 1497
AcsI	RAATTY	1 cut(s)	134
AcuI	CTGAAG	1 cut(s)	1472
AfaI	GTAC	4 cut(s)	1153, 1190, 1850, 2026
AfiI	CCNNNNNNNGG	1 cut(s)	27
AflII	CTTAAG	1 cut(s)	428
AhlI	ACTAGT	2 cut(s)	443, 800
AjnI	CCWGG	1 cut(s)	339
AjuI	GAANNNNNNNTTGG	2 cut(s)	875, 907
AloI	GAACNNNNNNTCC	2 cut(s)	1246, 1278
Alw21I	GWGCWC	2 cut(s)	938, 1460
Alw26I	GTCTC	2 cut(s)	94, 1957
Alw44I	GTGCAC	1 cut(s)	934
AlwI	GGATC	1 cut(s)	1256
AlwNI	CAGNNNCTG	1 cut(s)	1243
Ama87I	CYCGRG	1 cut(s)	284
Aor13HI	TCCGGA	1 cut(s)	1030
AoxI	GGCC	3 cut(s)	147, 208, 1497
ApaLI	GTGCAC	1 cut(s)	934
ApeKI	GCWGC	2 cut(s)	1237, 1657
ApoI	RAATTY	1 cut(s)	134
ArsI	GACNNNNNNTTYG	2 cut(s)	1320, 1352
Asp700I	GAANNNNTTC	1 cut(s)	998
AspLEI	GCGC	1 cut(s)	667
AspS9I	GGNCC	3 cut(s)	147, 264, 1024
AsuHPI	GGTGA	3 cut(s)	688, 892, 923
AvaI	CYCGRG	1 cut(s)	284
AvaII	GGWCC	2 cut(s)	264, 1024
BaeGI	GKGCMC	3 cut(s)	938, 1296, 1525
BanII	GRGCYC	1 cut(s)	1460
BbsI	GAAGAC	2 cut(s)	39, 1902
Bbv12I	GWGCWC	2 cut(s)	938, 1460
BbvCI	CCTCAGC	1 cut(s)	1095
BbvI	GCAGC	2 cut(s)	1249, 1644
BccI	CCATC	5 cut(s)	80, 370, 626, 1276, 1388
BceAI	ACGGC	1 cut(s)	1873
BciT130I	CCWGG	1 cut(s)	341
BciVI	GTATCC	1 cut(s)	1664
BclI	TGATCA	1 cut(s)	1284
BcoDI	GTCTC	2 cut(s)	94, 1957
BcuI	ACTAGT	2 cut(s)	443, 800
BfaI	CTAG	7 cut(s)	234, 444, 801, 872, 1334, 1439, 1916
BfmI	CTRYAG	2 cut(s)	1297, 1757
BfrI	CTTAAG	1 cut(s)	428
BfuAI	ACCTGC	1 cut(s)	1916
BfuI	GTATCC	1 cut(s)	1664
BglII	AGATCT	1 cut(s)	1594
BisI	GCNGC	4 cut(s)	231, 1238, 1500, 1658
BlsI	GCNGC	4 cut(s)	232, 1239, 1501, 1659
BmcAI	AGTACT	1 cut(s)	1190
Bme1390I	CCNGG	1 cut(s)	341
Bme18I	GGWCC	2 cut(s)	264, 1024
BmeT110I	CYCGRG	1 cut(s)	284
BmgT120I	GGNCC	3 cut(s)	147, 264, 1024
BmiI	GGNNCC	2 cut(s)	215, 1026
BmrFI	CCNGG	1 cut(s)	341
BmrI	ACTGGG	1 cut(s)	154
BmsI	GCATC	2 cut(s)	1437, 1644
BmuI	ACTGGG	1 cut(s)	154
BpiI	GAAGAC	2 cut(s)	39, 1902
BpmI	CTGGAG	1 cut(s)	26
Bpu10I	CCTNAGC	1 cut(s)	1095
BpuEI	CTTGAG	1 cut(s)	218
BsaAI	YACGTR	1 cut(s)	933
BsaJI	CCNNGG	2 cut(s)	26, 546
BsaWI	WCCGGW	2 cut(s)	216, 1030
Bsc4I	CCNNNNNNNGG	1 cut(s)	27
Bse1I	ACTGG	3 cut(s)	43, 149, 1500
Bse3DI	GCAATG	2 cut(s)	961, 973
BseAI	TCCGGA	1 cut(s)	1030
BseBI	CCWGG	1 cut(s)	341
BseDI	CCNNGG	2 cut(s)	26, 546
BseGI	GGATG	6 cut(s)	486, 610, 637, 690, 1268, 1659
BseLI	CCNNNNNNNGG	1 cut(s)	27
BseMI	GCAATG	2 cut(s)	961, 973
BseMII	CTCAG	1 cut(s)	1086
BseNI	ACTGG	3 cut(s)	43, 149, 1500
BseRI	GAGGAG	2 cut(s)	806, 1112
BseSI	GKGCMC	3 cut(s)	938, 1296, 1525
BseX3I	CGGCCG	1 cut(s)	208
BseXI	GCAGC	2 cut(s)	1249, 1644
Bsh1236I	CGCG	1 cut(s)	667
Bsh1285I	CGRYCG	1 cut(s)	211
BshFI	GGCC	3 cut(s)	149, 210, 1499
BsiEI	CGRYCG	1 cut(s)	211
BsiHKAI	GWGCWC	2 cut(s)	938, 1460
BsiHKCI	CYCGRG	1 cut(s)	284
BsiSI	CCGG	4 cut(s)	211, 217, 602, 1031
BslFI	GGGAC	2 cut(s)	376, 1010
BslI	CCNNNNNNNGG	1 cut(s)	27
BsmAI	GTCTC	2 cut(s)	94, 1957
BsmBI	CGTCTC	1 cut(s)	94
BsmFI	GGGAC	2 cut(s)	376, 1010
BsmI	GAATGC	2 cut(s)	955, 1968
BsnI	GGCC	3 cut(s)	149, 210, 1499
BsoBI	CYCGRG	1 cut(s)	284
Bsp1286I	GDGCHC	4 cut(s)	938, 1296, 1460, 1525
Bsp13I	TCCGGA	1 cut(s)	1030
Bsp1407I	TGTACA	2 cut(s)	1151, 2024
Bsp143I	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
Bsp19I	CCATGG	2 cut(s)	26, 546
BspACI	CCGC	3 cut(s)	103, 231, 1500
BspANI	GGCC	3 cut(s)	149, 210, 1499
BspCNI	CTCAG	1 cut(s)	1087
BspEI	TCCGGA	1 cut(s)	1030
BspFNI	CGCG	1 cut(s)	667
BspLI	GGNNCC	2 cut(s)	215, 1026
BspMAI	CTGCAG	1 cut(s)	1761
BspMI	ACCTGC	1 cut(s)	1916
BspPI	GGATC	1 cut(s)	1256
BspQI	GCTCTTC	1 cut(s)	1448
BspTI	CTTAAG	1 cut(s)	428
BsrBI	CCGCTC	1 cut(s)	1502
BsrDI	GCAATG	2 cut(s)	961, 973
BsrGI	TGTACA	2 cut(s)	1151, 2024
BsrI	ACTGG	3 cut(s)	43, 149, 1500
BssECI	CCNNGG	2 cut(s)	26, 546
BssMI	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
BssT1I	CCWWGG	2 cut(s)	26, 546
Bst2UI	CCWGG	1 cut(s)	341
Bst4CI	ACNGT	2 cut(s)	88, 1298
Bst6I	CTCTTC	5 cut(s)	61, 254, 686, 1072, 1448
BstAFI	CTTAAG	1 cut(s)	428
BstAUI	TGTACA	2 cut(s)	1151, 2024
BstBAI	YACGTR	1 cut(s)	933
BstC8I	GCNNGC	2 cut(s)	1321, 1537
BstDEI	CTNAG	6 cut(s)	453, 979, 1095, 1212, 1346, 2012
BstDSI	CCRYGG	2 cut(s)	26, 546
BstF5I	GGATG	6 cut(s)	486, 610, 637, 690, 1268, 1659
BstFNI	CGCG	1 cut(s)	667
BstHHI	GCGC	1 cut(s)	667
BstKTI	GATC	5 cut(s)	1144, 1264, 1287, 1597, 1934
BstMAI	GTCTC	2 cut(s)	94, 1957
BstMBI	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
BstMCI	CGRYCG	1 cut(s)	211
BstMWI	GCNNNNNNNGC	2 cut(s)	502, 850
BstNI	CCWGG	1 cut(s)	341
BstNSI	RCATGY	1 cut(s)	1737
BstSCI	CCNGG	1 cut(s)	339
BstSFI	CTRYAG	2 cut(s)	1297, 1757
BstSLI	GKGCMC	3 cut(s)	938, 1296, 1525
BstUI	CGCG	1 cut(s)	667
BstV1I	GCAGC	2 cut(s)	1249, 1644
BstV2I	GAAGAC	2 cut(s)	39, 1902
BstX2I	RGATCY	2 cut(s)	1261, 1594
BstYI	RGATCY	2 cut(s)	1261, 1594
BstZI	CGGCCG	1 cut(s)	208
BsuI	GTATCC	1 cut(s)	1664
BsuRI	GGCC	3 cut(s)	149, 210, 1499
BtgI	CCRYGG	2 cut(s)	26, 546
BtsCI	GGATG	6 cut(s)	486, 610, 637, 690, 1268, 1659
BtsIMutI	CAGTG	2 cut(s)	1294, 1621
BveI	ACCTGC	1 cut(s)	1916
Cac8I	GCNNGC	2 cut(s)	1321, 1537
CaiI	CAGNNNCTG	1 cut(s)	1243
CfoI	GCGC	1 cut(s)	667
Cfr13I	GGNCC	3 cut(s)	147, 264, 1024
Csp6I	GTAC	4 cut(s)	1152, 1189, 1849, 2025
CviAII	CATG	8 cut(s)	27, 190, 547, 952, 1288, 1661, 1734, 2019
CviQI	GTAC	4 cut(s)	1152, 1189, 1849, 2025
DdeI	CTNAG	6 cut(s)	453, 979, 1095, 1212, 1346, 2012
DpnI	GATC	5 cut(s)	1143, 1263, 1286, 1596, 1933
DpnII	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
DraI	TTTAAA	1 cut(s)	1905
EaeI	YGGCCR	2 cut(s)	208, 1497
EagI	CGGCCG	1 cut(s)	208
Eam1104I	CTCTTC	5 cut(s)	61, 254, 686, 1072, 1448
EarI	CTCTTC	5 cut(s)	61, 254, 686, 1072, 1448
EciI	GGCGGA	1 cut(s)	92
Ecl136II	GAGCTC	1 cut(s)	1458
EclXI	CGGCCG	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	26, 546
Eco24I	GRGCYC	1 cut(s)	1460
Eco32I	GATATC	1 cut(s)	384
Eco47I	GGWCC	2 cut(s)	264, 1024
Eco52I	CGGCCG	1 cut(s)	208
Eco53kI	GAGCTC	1 cut(s)	1458
Eco57I	CTGAAG	1 cut(s)	1472
Eco88I	CYCGRG	1 cut(s)	284
EcoICRI	GAGCTC	1 cut(s)	1458
EcoO109I	RGGNCCY	1 cut(s)	1024
EcoRII	CCWGG	1 cut(s)	339
EcoRV	GATATC	1 cut(s)	384
EcoT14I	CCWWGG	2 cut(s)	26, 546
EcoT22I	ATGCAT	2 cut(s)	957, 1518
EcoT38I	GRGCYC	1 cut(s)	1460
ErhI	CCWWGG	2 cut(s)	26, 546
Esp3I	CGTCTC	1 cut(s)	94
FaeI	CATG	8 cut(s)	30, 193, 550, 955, 1291, 1664, 1737, 2022
FaqI	GGGAC	2 cut(s)	376, 1010
FatI	CATG	8 cut(s)	26, 189, 546, 951, 1287, 1660, 1733, 2018
FbaI	TGATCA	1 cut(s)	1284
Fnu4HI	GCNGC	4 cut(s)	231, 1238, 1500, 1658
FokI	GGATG	6 cut(s)	493, 617, 644, 697, 1255, 1666
FriOI	GRGCYC	1 cut(s)	1460
Fsp4HI	GCNGC	4 cut(s)	231, 1238, 1500, 1658
FspBI	CTAG	7 cut(s)	234, 444, 801, 872, 1334, 1439, 1916
GlaI	GCGC	1 cut(s)	666
GluI	GCNGC	4 cut(s)	231, 1238, 1500, 1658
GsuI	CTGGAG	1 cut(s)	26
HaeIII	GGCC	3 cut(s)	149, 210, 1499
HapII	CCGG	4 cut(s)	211, 217, 602, 1031
HhaI	GCGC	1 cut(s)	667
Hin1II	CATG	8 cut(s)	30, 193, 550, 955, 1291, 1664, 1737, 2022
Hin6I	GCGC	1 cut(s)	665
HinP1I	GCGC	1 cut(s)	665
HindIII	AAGCTT	3 cut(s)	353, 1537, 1826
HinfI	GANTC	8 cut(s)	152, 220, 344, 709, 1034, 1382, 1435, 1529
HpaII	CCGG	4 cut(s)	211, 217, 602, 1031
HphI	GGTGA	3 cut(s)	688, 892, 923
Hpy166II	GTNNAC	3 cut(s)	936, 1154, 1984
Hpy188I	TCNGA	2 cut(s)	714, 1015
Hpy188III	TCNNGA	6 cut(s)	926, 1031, 1364, 1439, 1832, 2001
Hpy8I	GTNNAC	3 cut(s)	936, 1154, 1984
HpyAV	CCTTC	8 cut(s)	193, 268, 673, 908, 988, 1298, 1344, 1430
HpyCH4III	ACNGT	2 cut(s)	88, 1298
HpyCH4IV	ACGT	3 cut(s)	509, 932, 938
HpyF10VI	GCNNNNNNNGC	2 cut(s)	502, 850
HpyF3I	CTNAG	6 cut(s)	453, 979, 1095, 1212, 1346, 2012
HpySE526I	ACGT	3 cut(s)	509, 932, 938
Hsp92II	CATG	8 cut(s)	30, 193, 550, 955, 1291, 1664, 1737, 2022
HspAI	GCGC	1 cut(s)	665
Kpn2I	TCCGGA	1 cut(s)	1030
Ksp22I	TGATCA	1 cut(s)	1284
Kzo9I	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
LguI	GCTCTTC	1 cut(s)	1448
LmnI	GCTCC	1 cut(s)	315
Lsp1109I	GCAGC	2 cut(s)	1249, 1644
LweI	GCATC	2 cut(s)	1437, 1644
MaeI	CTAG	7 cut(s)	234, 444, 801, 872, 1334, 1439, 1916
MaeII	ACGT	3 cut(s)	509, 932, 938
MaeIII	GTNAC	4 cut(s)	490, 610, 721, 1017
MalI	GATC	5 cut(s)	1143, 1263, 1286, 1596, 1933
MbiI	CCGCTC	1 cut(s)	1502
MboI	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
MfeI	CAATTG	1 cut(s)	252
MflI	RGATCY	2 cut(s)	1261, 1594
MhlI	GDGCHC	4 cut(s)	938, 1296, 1460, 1525
MluCI	AATT	3 cut(s)	134, 252, 825
MlyI	GAGTC	2 cut(s)	353, 1429
MmeI	TCCRAC	3 cut(s)	45, 1383, 1536
Mph1103I	ATGCAT	2 cut(s)	957, 1518
MroI	TCCGGA	1 cut(s)	1030
MroXI	GAANNNNTTC	1 cut(s)	998
MslI	CAYNNNNRTG	3 cut(s)	950, 966, 1007
MspA1I	CMGCKG	1 cut(s)	1978
MspCI	CTTAAG	1 cut(s)	428
MspI	CCGG	4 cut(s)	211, 217, 602, 1031
MspR9I	CCNGG	1 cut(s)	341
MunI	CAATTG	1 cut(s)	252
Mva1269I	GAATGC	2 cut(s)	955, 1968
MvaI	CCWGG	1 cut(s)	341
MvnI	CGCG	1 cut(s)	667
MwoI	GCNNNNNNNGC	2 cut(s)	502, 850
NcoI	CCATGG	2 cut(s)	26, 546
NdeII	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
NlaIII	CATG	8 cut(s)	30, 193, 550, 955, 1291, 1664, 1737, 2022
NlaIV	GGNNCC	2 cut(s)	215, 1026
NsiI	ATGCAT	2 cut(s)	957, 1518
NspI	RCATGY	1 cut(s)	1737
PaeR7I	CTCGAG	1 cut(s)	284
PciSI	GCTCTTC	1 cut(s)	1448
PcsI	WCGNNNNNNNCGW	1 cut(s)	371
PctI	GAATGC	2 cut(s)	955, 1968
PdmI	GAANNNNTTC	1 cut(s)	998
PfeI	GAWTC	6 cut(s)	152, 220, 709, 1034, 1382, 1529
PflFI	GACNNNGTC	1 cut(s)	1949
PflMI	CCANNNNNTGG	1 cut(s)	27
PfoI	TCCNGGA	1 cut(s)	339
PkrI	GCNGC	4 cut(s)	232, 1239, 1501, 1659
PleI	GAGTC	2 cut(s)	352, 1429
PpsI	GAGTC	2 cut(s)	352, 1429
Ppu21I	YACGTR	1 cut(s)	933
PpuMI	RGGWCCY	1 cut(s)	1024
PsiI	TTATAA	2 cut(s)	618, 1601
Psp124BI	GAGCTC	1 cut(s)	1460
Psp5II	RGGWCCY	1 cut(s)	1024
Psp6I	CCWGG	1 cut(s)	339
PspGI	CCWGG	1 cut(s)	339
PspN4I	GGNNCC	2 cut(s)	215, 1026
PspPI	GGNCC	3 cut(s)	147, 264, 1024
PspPPI	RGGWCCY	1 cut(s)	1024
PspXI	VCTCGAGB	1 cut(s)	284
PstI	CTGCAG	1 cut(s)	1761
PstNI	CAGNNNCTG	1 cut(s)	1243
PsuI	RGATCY	2 cut(s)	1261, 1594
PsyI	GACNNNGTC	1 cut(s)	1949
PvuII	CAGCTG	1 cut(s)	1978
RsaI	GTAC	4 cut(s)	1153, 1190, 1850, 2026
RsaNI	GTAC	4 cut(s)	1152, 1189, 1849, 2025
RseI	CAYNNNNRTG	3 cut(s)	950, 966, 1007
SacI	GAGCTC	1 cut(s)	1460
SapI	GCTCTTC	1 cut(s)	1448
SatI	GCNGC	4 cut(s)	231, 1238, 1500, 1658
Sau3AI	GATC	5 cut(s)	1141, 1261, 1284, 1594, 1931
Sau96I	GGNCC	3 cut(s)	147, 264, 1024
ScaI	AGTACT	1 cut(s)	1190
SchI	GAGTC	2 cut(s)	353, 1429
ScrFI	CCNGG	1 cut(s)	341
SduI	GDGCHC	4 cut(s)	938, 1296, 1460, 1525
SfaNI	GCATC	2 cut(s)	1437, 1644
SfcI	CTRYAG	2 cut(s)	1297, 1757
Sfr274I	CTCGAG	1 cut(s)	284
SinI	GGWCC	2 cut(s)	264, 1024
SlaI	CTCGAG	1 cut(s)	284
SmiMI	CAYNNNNRTG	3 cut(s)	950, 966, 1007
SmlI	CTYRAG	3 cut(s)	197, 284, 428
SmoI	CTYRAG	3 cut(s)	197, 284, 428
SpeI	ACTAGT	2 cut(s)	443, 800
Sse9I	AATT	3 cut(s)	134, 252, 825
SsiI	CCGC	3 cut(s)	103, 231, 1500
SspI	AATATT	3 cut(s)	517, 1579, 1743
SspMI	CTAG	7 cut(s)	234, 444, 801, 872, 1334, 1439, 1916
SstI	GAGCTC	1 cut(s)	1460
StyD4I	CCNGG	1 cut(s)	339
StyI	CCWWGG	2 cut(s)	26, 546
TaaI	ACNGT	2 cut(s)	88, 1298
TaiI	ACGT	3 cut(s)	512, 935, 941
TaqI	TCGA	4 cut(s)	285, 419, 1676, 1831
TasI	AATT	3 cut(s)	134, 252, 825
TatI	WGTACW	4 cut(s)	1151, 1188, 1848, 2024
TauI	GCSGC	2 cut(s)	233, 1502
TfiI	GAWTC	6 cut(s)	152, 220, 709, 1034, 1382, 1529
TscAI	CASTG	2 cut(s)	1301, 1621
TseI	GCWGC	2 cut(s)	1237, 1657
TspDTI	ATGAA	8 cut(s)	178, 383, 483, 554, 554, 837, 1239, 1869
TspGWI	ACGGA	1 cut(s)	1683
TspRI	CASTG	2 cut(s)	1301, 1621
Tth111I	GACNNNGTC	1 cut(s)	1949
Van91I	CCANNNNNTGG	1 cut(s)	27
Vha464I	CTTAAG	1 cut(s)	428
VneI	GTGCAC	1 cut(s)	934
VpaK11BI	GGWCC	2 cut(s)	264, 1024
XapI	RAATTY	1 cut(s)	134
XbaI	TCTAGA	1 cut(s)	1438
XceI	RCATGY	1 cut(s)	1737
XhoI	CTCGAG	1 cut(s)	284
XmnI	GAANNNNTTC	1 cut(s)	998
XspI	CTAG	7 cut(s)	234, 444, 801, 872, 1334, 1439, 1916
ZrmI	AGTACT	1 cut(s)	1190
Zsp2I	ATGCAT	2 cut(s)	957, 1518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.