Rw1G016620

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005468
Physical Location & Seq
Forward (+)
36851965 .. 36855532
3568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016620.1

Sequence Viewer

Length: 2247 bp
ATGGCCGCCAAACCCATTTCCCGGCGACCAGCCTTGACAGTTCTCCGAGAGCAATTCCTCAGACCCAACCGCAACTCCAAACCTTCTCCTCCTCCTCCTCCTCTCAGGTCCTCCGATGCCGAAAAGCCCAAATCCCAAGGCAGCAAAACCGCCAAAGCCATGGCGTCGCTCATCAACTCCAACCCGTGGTCATCTCACCTCCAGTCTTCTCTCTCTTCTCTCTCTCCCTCCATCTCCACCACCACCGTCTCCCAAACCCTCCGCCGCATCAAAACCCCAACCCAAGCCCTCAAGTTCTTCAACTGGGCCGAATCAATGGGCTTCTCCCACAACGCCCAATCCTTCTTCATGTTGCTTGAGATCTTCGGCCGGAACCGGAATCTCAATGCCGCTAGAAATCTCTTGTTTTCGATTGAGAAGAGGTCCAATGGGAAGGTTAAGCTCGAGGACCGGTTTTTTAATAGCTTGATTAGGAGCTATGGGGAGGCAGGGTTGTTCCAGGAGTCTGTAAAGCTTTTTGGGACGATGAAGTCAATGGGGATATCGGCTTCTGTTGTTACTTTCAATAGTTTGCTTTCGATTTTGCTTAAGAGGGGTAGGACTAGTATGGCTAAGAATGTTTATGATGAAATGCTTGGGATGTATGGAGTTACGCCGGATACGCATACGTTTAATATTTTGATTAGAGGGTTCTGTATGAACTCCATGGTTGATGAGGGGTTTCATTATTTCAAGGAAATGTCACGGTTGAAATGTGAGCCGGATGTGGTTACTTATAATACGATTGTGGATGGGTTGTGTAGAGCAGGGAAGGTTGAGATTGCGCGAAATGTGGTGAAGGGTATGAGGAAGAGAAGTGGGGAGTTGAATCCGAATGTTGTTACTTATACGACATTGATTAGAGGGTATTGTGTGAAAGAAGATGTTGATGAGGCTTTGTGTGTTTTGGAGGAGATGACTAGTCAAGGGTTGAAACCAAATGAAATTACCTATAATACTCTGCTTAAAGGGCTTTGTGAAGCAAAGAAGCTAGATAAGGTGAAAGAAGTCTTGGAGGGAACAATGAGAGGTGAAGGATTTACTCCTGATACGTGCACGTTCAACACGCTCATGCATTCGCATTGCAATGCAGGAAACTTAGAGGAAGCATTGAAGGTTTTCACAATGATGTCAGAGTTACAGGTCCCTCCGGATTCAGCTACATACAGCGTTTTGATACGCAGTTTGTGTCAGCGAAGAGATTATGGTAAAGCTGAGGAGTTGTTTGATGAGCTATCCAAGAAACAAATCTTGTTAAGTGATCGTGGCTGTACACCTGTTGTTGCCTCATACAAACCTATCTTTGAGTACTTATGTAGTAATGGGAGAACTAAGAAAGCAGATGAAGTGTTTAGGCAGCTACTGAAAAGAGGAACACAAGATCCTCCATCCTACAAGACTTTGATCATGGGGCACTGTAGGGAAAGCACATTTGAAGCTGGCTACAAACTCCTAGTCTTTATGCTTAGAAGGGATTTTGTTCCTGATGTTGAGATTTATGATTCCTTGATTGATGGTCTGTTGGAAAAGGGTAATCCCCTTCTTGCACAACAGACTCTAGAGAAGATGCTGAAGAGCTCTCATCTCCCAAAAACATCTACTTTCCATTCTATACTCGCCGCTCTTTTAGAAATGCATTGTGCCCGTGAATCTGCAAGCTTTATTATTTTAATGTTGGAGAAAAACATTAGACAAAATATTAACCTCTCAACAGATCTTATAAGGCTTCTTTTCAGTGAGGGATTGCGAGATAAAGCATTTGAGGTTGTAGGGATGCTGCATGATAACGGATACTCGATTAAAATAGAAGAAGTGGTCAGTTTCCTTTACCAGAGCAGAAAGCTAATAGAGGCATGTGAAATATTGCGATTTAGTCTGCAGAAGCAAGAAAATGTCAGTATTGATATATTTGACCAAGTTATATTAGGGCTATGTGATATAAATAAGCTTCGAGAAGCATTTGGCTTGTACTATGAACTGATTGAGAATGGCGACCACCAGCAGTTGCCGTGCTTACATCATTTAAAGTCTTCCCTAGAGGTTGCAGGTAGATCAGTTGAAGCTGACTTTGTCTCTAAAAGAATGCCAAAACATCAGCTGGTGGACAAATGTGGGAAGTCAAGACCTCAACTATGCTACCCACTTCTCTTCGGCGCTTGCAGATTTTGTGTTGCCCTGCACCCCTGCTCTGCAGTGCTTGCCAGATAG

Protein Analysis

748

Amino Acids

84.38

Weight (kDa)

8.96

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 153 - 192 4.9e-07 PPR repeat family
PPR_2 PF13041 184 - 232 2.5e-13 PPR repeat family
PPR_long PF17177 206 - 354 1.1e-13 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 216 - 248 4e-08 PPR repeat
PPR_2 PF13041 219 - 268 3.8e-16 PPR repeat family
PPR PF01535 223 - 252 8.8e-06 PPR repeat
PPR_1 PF12854 252 - 283 2.2e-12 PPR repeat
PPR_2 PF13041 254 - 287 1.5e-07 PPR repeat family
TPR_24 PF23276 268 - 352 1.5e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 289 - 320 5.6e-11 PPR repeat
PPR_3 PF13812 289 - 336 7.6e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 291 - 339 1.7e-19 PPR repeat family
PPR PF01535 294 - 324 1.2e-07 PPR repeat
PPR_3 PF13812 315 - 371 1.3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 323 - 351 9.8e-08 PPR repeat
PPR_3 PF13812 355 - 407 1.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 359 - 390 1.5e-10 PPR repeat
PPR_2 PF13041 362 - 411 2e-16 PPR repeat family
PPR PF01535 366 - 393 3.2e-06 PPR repeat
PPR_1 PF12854 395 - 424 6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 777, 1760
AasI GACNNNNNNGTC 1 cut(s) 529
Acc36I ACCTGC 1 cut(s) 2075
AccB7I CCANNNNNTGG 1 cut(s) 186
AccBSI CCGCTC 1 cut(s) 1661
AccII CGCG 1 cut(s) 826
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 7 cut(s) 6, 70, 150, 262, 265, 390, 1659
AclWI GGATC 1 cut(s) 1415
AcoI YGGCCR 2 cut(s) 3, 367
AcuI CTGAAG 1 cut(s) 1631
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 3 cut(s) 1312, 1349, 2009
AfiI CCNNNNNNNGG 2 cut(s) 21, 186
AflII CTTAAG 1 cut(s) 587
AgeI ACCGGT 1 cut(s) 450
AhlI ACTAGT 2 cut(s) 602, 959
AjnI CCWGG 1 cut(s) 498
AjuI GAANNNNNNNTTGG 2 cut(s) 1034, 1066
AloI GAACNNNNNNTCC 2 cut(s) 1405, 1437
Alw21I GWGCWC 2 cut(s) 1097, 1619
Alw26I GTCTC 2 cut(s) 253, 2116
Alw44I GTGCAC 1 cut(s) 1093
AlwI GGATC 1 cut(s) 1415
AlwNI CAGNNNCTG 1 cut(s) 1402
Ama87I CYCGRG 1 cut(s) 443
Aor13HI TCCGGA 1 cut(s) 1189
AoxI GGCC 3 cut(s) 3, 306, 367
ApaLI GTGCAC 1 cut(s) 1093
ApeKI GCWGC 3 cut(s) 141, 1396, 1816
ArsI GACNNNNNNTTYG 2 cut(s) 1479, 1511
AsiGI ACCGGT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 1157
AspLEI GCGC 2 cut(s) 826, 2195
AspS9I GGNCC 5 cut(s) 108, 306, 423, 448, 1183
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 4 cut(s) 188, 847, 1051, 1082
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 4 cut(s) 108, 423, 448, 1183
BaeGI GKGCMC 3 cut(s) 1097, 1455, 1684
BanII GRGCYC 1 cut(s) 1619
BbsI GAAGAC 2 cut(s) 198, 2061
Bbv12I GWGCWC 2 cut(s) 1097, 1619
BbvCI CCTCAGC 1 cut(s) 1254
BbvI GCAGC 3 cut(s) 153, 1408, 1803
BccI CCATC 4 cut(s) 239, 785, 1435, 1547
BceAI ACGGC 1 cut(s) 2032
BciT130I CCWGG 1 cut(s) 500
BciVI GTATCC 2 cut(s) 652, 1823
BclI TGATCA 1 cut(s) 1443
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 2 cut(s) 253, 2116
BcuI ACTAGT 2 cut(s) 602, 959
BfaI CTAG 7 cut(s) 393, 603, 960, 1031, 1493, 1598, 2075
BfmI CTRYAG 3 cut(s) 1456, 1916, 2229
BfoI RGCGCY 1 cut(s) 2196
BfrI CTTAAG 1 cut(s) 587
BfuAI ACCTGC 1 cut(s) 2075
BfuI GTATCC 2 cut(s) 652, 1823
BglII AGATCT 2 cut(s) 360, 1753
BisI GCNGC 7 cut(s) 6, 142, 265, 390, 1397, 1659, 1817
BlsI GCNGC 7 cut(s) 7, 143, 266, 391, 1398, 1660, 1818
BmcAI AGTACT 1 cut(s) 1349
Bme1390I CCNGG 2 cut(s) 22, 500
Bme18I GGWCC 4 cut(s) 108, 423, 448, 1183
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 5 cut(s) 108, 306, 423, 448, 1183
BmiI GGNNCC 2 cut(s) 374, 1185
BmrFI CCNGG 2 cut(s) 22, 500
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 4 cut(s) 106, 276, 1596, 1803
BmuI ACTGGG 1 cut(s) 313
BpiI GAAGAC 2 cut(s) 198, 2061
BpmI CTGGAG 1 cut(s) 185
Bpu10I CCTNAGC 1 cut(s) 1254
BpuEI CTTGAG 2 cut(s) 275, 377
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 1092
BsaHI GRCGYC 1 cut(s) 164
BsaJI CCNNGG 4 cut(s) 136, 159, 185, 705
BsaWI WCCGGW 3 cut(s) 375, 450, 1189
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 476, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 186
Bse118I RCCGGY 1 cut(s) 450
Bse1I ACTGG 2 cut(s) 202, 308
Bse3DI GCAATG 2 cut(s) 1120, 1132
BseAI TCCGGA 1 cut(s) 1189
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 4 cut(s) 136, 159, 185, 705
BseGI GGATG 5 cut(s) 645, 769, 796, 1427, 1818
BseLI CCNNNNNNNGG 2 cut(s) 21, 186
BseMI GCAATG 2 cut(s) 1120, 1132
BseMII CTCAG 3 cut(s) 73, 118, 1245
BseNI ACTGG 2 cut(s) 202, 308
BseRI GAGGAG 7 cut(s) 78, 81, 84, 87, 90, 965, 1271
BseSI GKGCMC 3 cut(s) 1097, 1455, 1684
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 3 cut(s) 153, 1408, 1803
BsgI GTGCAG 1 cut(s) 2201
Bsh1236I CGCG 1 cut(s) 826
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 5, 308, 369
BshTI ACCGGT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 2 cut(s) 1097, 1619
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 7 cut(s) 22, 370, 376, 451, 656, 761, 1190
BslFI GGGAC 2 cut(s) 535, 1169
BslI CCNNNNNNNGG 2 cut(s) 21, 186
BsmAI GTCTC 2 cut(s) 253, 2116
BsmBI CGTCTC 1 cut(s) 253
BsmFI GGGAC 2 cut(s) 535, 1169
BsmI GAATGC 2 cut(s) 1114, 2127
BsnI GGCC 3 cut(s) 5, 308, 369
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 4 cut(s) 1097, 1455, 1619, 1684
Bsp13I TCCGGA 1 cut(s) 1189
Bsp1407I TGTACA 1 cut(s) 1310
Bsp143I GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
Bsp19I CCATGG 2 cut(s) 159, 705
BspACI CCGC 7 cut(s) 6, 70, 150, 262, 265, 390, 1659
BspANI GGCC 3 cut(s) 5, 308, 369
BspCNI CTCAG 3 cut(s) 72, 117, 1246
BspEI TCCGGA 1 cut(s) 1189
BspFNI CGCG 1 cut(s) 826
BspLI GGNNCC 2 cut(s) 374, 1185
BspMAI CTGCAG 2 cut(s) 1920, 2233
BspMI ACCTGC 1 cut(s) 2075
BspPI GGATC 1 cut(s) 1415
BspQI GCTCTTC 1 cut(s) 1607
BspTI CTTAAG 1 cut(s) 587
BsrBI CCGCTC 1 cut(s) 1661
BsrDI GCAATG 2 cut(s) 1120, 1132
BsrFI RCCGGY 1 cut(s) 450
BsrGI TGTACA 1 cut(s) 1310
BsrI ACTGG 2 cut(s) 202, 308
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 4 cut(s) 136, 159, 185, 705
BssMI GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 3 cut(s) 136, 159, 705
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 4 cut(s) 40, 247, 747, 1457
Bst6I CTCTTC 6 cut(s) 220, 413, 845, 1231, 1607, 2192
BstACI GRCGYC 1 cut(s) 164
BstAFI CTTAAG 1 cut(s) 587
BstAPI GCANNNNNTGC 1 cut(s) 2237
BstAUI TGTACA 1 cut(s) 1310
BstBAI YACGTR 1 cut(s) 1092
BstC8I GCNNGC 4 cut(s) 1480, 1696, 2197, 2238
BstDEI CTNAG 7 cut(s) 59, 104, 612, 1138, 1254, 1371, 1505
BstDSI CCRYGG 3 cut(s) 159, 185, 705
BstF5I GGATG 5 cut(s) 645, 769, 796, 1427, 1818
BstFNI CGCG 1 cut(s) 826
BstH2I RGCGCY 1 cut(s) 2196
BstHHI GCGC 2 cut(s) 826, 2195
BstKTI GATC 6 cut(s) 363, 1303, 1423, 1446, 1756, 2093
BstMAI GTCTC 2 cut(s) 253, 2116
BstMBI GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 3 cut(s) 661, 1009, 2237
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 1 cut(s) 1896
BstSCI CCNGG 2 cut(s) 20, 498
BstSFI CTRYAG 3 cut(s) 1456, 1916, 2229
BstSLI GKGCMC 3 cut(s) 1097, 1455, 1684
BstUI CGCG 1 cut(s) 826
BstV1I GCAGC 3 cut(s) 153, 1408, 1803
BstV2I GAAGAC 2 cut(s) 198, 2061
BstX2I RGATCY 3 cut(s) 360, 1420, 1753
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 3 cut(s) 360, 1420, 1753
BstZI CGGCCG 1 cut(s) 367
BsuI GTATCC 2 cut(s) 652, 1823
BsuRI GGCC 3 cut(s) 5, 308, 369
BtgI CCRYGG 3 cut(s) 159, 185, 705
BtsCI GGATG 5 cut(s) 645, 769, 796, 1427, 1818
BtsI GCAGTG 1 cut(s) 2238
BtsIMutI CAGTG 3 cut(s) 1453, 1780, 2238
BveI ACCTGC 1 cut(s) 2075
Cac8I GCNNGC 4 cut(s) 1480, 1696, 2197, 2238
CaiI CAGNNNCTG 1 cut(s) 1402
CfoI GCGC 2 cut(s) 826, 2195
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 5 cut(s) 108, 306, 423, 448, 1183
CseI GACGC 1 cut(s) 153
Csp6I GTAC 3 cut(s) 1311, 1348, 2008
CspAI ACCGGT 1 cut(s) 450
CviAII CATG 7 cut(s) 160, 349, 706, 1111, 1447, 1820, 1893
CviQI GTAC 3 cut(s) 1311, 1348, 2008
DdeI CTNAG 7 cut(s) 59, 104, 612, 1138, 1254, 1371, 1505
DpnI GATC 6 cut(s) 362, 1302, 1422, 1445, 1755, 2092
DpnII GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
DraI TTTAAA 1 cut(s) 2064
DrdI GACNNNNNNGTC 1 cut(s) 529
DseDI GACNNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 2 cut(s) 3, 367
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 6 cut(s) 220, 413, 845, 1231, 1607, 2192
EarI CTCTTC 6 cut(s) 220, 413, 845, 1231, 1607, 2192
EciI GGCGGA 1 cut(s) 251
Ecl136II GAGCTC 1 cut(s) 1617
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 3 cut(s) 136, 159, 705
Eco24I GRGCYC 1 cut(s) 1619
Eco32I GATATC 1 cut(s) 543
Eco47I GGWCC 4 cut(s) 108, 423, 448, 1183
Eco52I CGGCCG 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 1617
Eco57I CTGAAG 1 cut(s) 1631
Eco88I CYCGRG 1 cut(s) 443
EcoICRI GAGCTC 1 cut(s) 1617
EcoO109I RGGNCCY 2 cut(s) 108, 1183
EcoRII CCWGG 1 cut(s) 498
EcoRV GATATC 1 cut(s) 543
EcoT14I CCWWGG 3 cut(s) 136, 159, 705
EcoT22I ATGCAT 2 cut(s) 1116, 1677
EcoT38I GRGCYC 1 cut(s) 1619
ErhI CCWWGG 3 cut(s) 136, 159, 705
Esp3I CGTCTC 1 cut(s) 253
FaeI CATG 7 cut(s) 163, 352, 709, 1114, 1450, 1823, 1896
FaqI GGGAC 2 cut(s) 535, 1169
FatI CATG 7 cut(s) 159, 348, 705, 1110, 1446, 1819, 1892
FbaI TGATCA 1 cut(s) 1443
Fnu4HI GCNGC 7 cut(s) 6, 142, 265, 390, 1397, 1659, 1817
FokI GGATG 5 cut(s) 652, 776, 803, 1414, 1825
FriOI GRGCYC 1 cut(s) 1619
Fsp4HI GCNGC 7 cut(s) 6, 142, 265, 390, 1397, 1659, 1817
FspBI CTAG 7 cut(s) 393, 603, 960, 1031, 1493, 1598, 2075
GlaI GCGC 2 cut(s) 825, 2194
GluI GCNGC 7 cut(s) 6, 142, 265, 390, 1397, 1659, 1817
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 2196
HaeIII GGCC 3 cut(s) 5, 308, 369
HapII CCGG 7 cut(s) 22, 370, 376, 451, 656, 761, 1190
HgaI GACGC 1 cut(s) 153
HhaI GCGC 2 cut(s) 826, 2195
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 7 cut(s) 163, 352, 709, 1114, 1450, 1823, 1896
Hin6I GCGC 2 cut(s) 824, 2193
HinP1I GCGC 2 cut(s) 824, 2193
HindIII AAGCTT 3 cut(s) 512, 1696, 1985
HinfI GANTC 8 cut(s) 311, 379, 503, 868, 1193, 1541, 1594, 1688
HpaII CCGG 7 cut(s) 22, 370, 376, 451, 656, 761, 1190
HphI GGTGA 4 cut(s) 188, 847, 1051, 1082
Hpy166II GTNNAC 3 cut(s) 1095, 1313, 2143
Hpy188I TCNGA 5 cut(s) 47, 62, 115, 873, 1174
Hpy188III TCNNGA 6 cut(s) 1085, 1190, 1523, 1598, 1991, 2160
Hpy8I GTNNAC 3 cut(s) 1095, 1313, 2143
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 9 cut(s) 93, 352, 427, 805, 832, 1067, 1147, 1503, 1589
HpyCH4III ACNGT 4 cut(s) 40, 247, 747, 1457
HpyCH4IV ACGT 3 cut(s) 668, 1091, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 661, 1009, 2237
HpyF3I CTNAG 7 cut(s) 59, 104, 612, 1138, 1254, 1371, 1505
HpySE526I ACGT 3 cut(s) 668, 1091, 1097
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 7 cut(s) 163, 352, 709, 1114, 1450, 1823, 1896
HspAI GCGC 2 cut(s) 824, 2193
Kpn2I TCCGGA 1 cut(s) 1189
Ksp22I TGATCA 1 cut(s) 1443
Kzo9I GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
LguI GCTCTTC 1 cut(s) 1607
LmnI GCTCC 1 cut(s) 474
Lsp1109I GCAGC 3 cut(s) 153, 1408, 1803
LweI GCATC 4 cut(s) 106, 276, 1596, 1803
MaeI CTAG 7 cut(s) 393, 603, 960, 1031, 1493, 1598, 2075
MaeII ACGT 3 cut(s) 668, 1091, 1097
MaeIII GTNAC 6 cut(s) 556, 649, 741, 769, 880, 1176
MalI GATC 6 cut(s) 362, 1302, 1422, 1445, 1755, 2092
MbiI CCGCTC 1 cut(s) 1661
MboI GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
MflI RGATCY 3 cut(s) 360, 1420, 1753
MhlI GDGCHC 4 cut(s) 1097, 1455, 1619, 1684
MluCI AATT 2 cut(s) 53, 984
MlyI GAGTC 2 cut(s) 512, 1588
MmeI TCCRAC 3 cut(s) 204, 1542, 1695
Mph1103I ATGCAT 2 cut(s) 1116, 1677
MroI TCCGGA 1 cut(s) 1189
MroXI GAANNNNTTC 1 cut(s) 1157
MslI CAYNNNNRTG 3 cut(s) 1109, 1125, 1166
MspA1I CMGCKG 1 cut(s) 2137
MspCI CTTAAG 1 cut(s) 587
MspI CCGG 7 cut(s) 22, 370, 376, 451, 656, 761, 1190
MspR9I CCNGG 2 cut(s) 22, 500
Mva1269I GAATGC 2 cut(s) 1114, 2127
MvaI CCWGG 1 cut(s) 500
MvnI CGCG 1 cut(s) 826
MwoI GCNNNNNNNGC 3 cut(s) 661, 1009, 2237
NciI CCSGG 1 cut(s) 22
NcoI CCATGG 2 cut(s) 159, 705
NdeII GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
NlaIII CATG 7 cut(s) 163, 352, 709, 1114, 1450, 1823, 1896
NlaIV GGNNCC 2 cut(s) 374, 1185
NmuCI GTSAC 1 cut(s) 741
NsiI ATGCAT 2 cut(s) 1116, 1677
NspI RCATGY 1 cut(s) 1896
PaeR7I CTCGAG 1 cut(s) 443
PciSI GCTCTTC 1 cut(s) 1607
PctI GAATGC 2 cut(s) 1114, 2127
PdmI GAANNNNTTC 1 cut(s) 1157
PfeI GAWTC 6 cut(s) 311, 379, 868, 1193, 1541, 1688
PflFI GACNNNGTC 1 cut(s) 2108
PflMI CCANNNNNTGG 1 cut(s) 186
PfoI TCCNGGA 1 cut(s) 498
PinAI ACCGGT 1 cut(s) 450
PkrI GCNGC 7 cut(s) 7, 143, 266, 391, 1398, 1660, 1818
PleI GAGTC 2 cut(s) 511, 1588
PpsI GAGTC 2 cut(s) 511, 1588
Ppu21I YACGTR 1 cut(s) 1092
PpuMI RGGWCCY 2 cut(s) 108, 1183
PsiI TTATAA 2 cut(s) 777, 1760
Psp124BI GAGCTC 1 cut(s) 1619
Psp5II RGGWCCY 2 cut(s) 108, 1183
Psp6I CCWGG 1 cut(s) 498
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 374, 1185
PspPI GGNCC 5 cut(s) 108, 306, 423, 448, 1183
PspPPI RGGWCCY 2 cut(s) 108, 1183
PspXI VCTCGAGB 1 cut(s) 443
PstI CTGCAG 2 cut(s) 1920, 2233
PstNI CAGNNNCTG 1 cut(s) 1402
PsuI RGATCY 3 cut(s) 360, 1420, 1753
PsyI GACNNNGTC 1 cut(s) 2108
PvuII CAGCTG 1 cut(s) 2137
RsaI GTAC 3 cut(s) 1312, 1349, 2009
RsaNI GTAC 3 cut(s) 1311, 1348, 2008
RseI CAYNNNNRTG 3 cut(s) 1109, 1125, 1166
SacI GAGCTC 1 cut(s) 1619
SapI GCTCTTC 1 cut(s) 1607
SatI GCNGC 7 cut(s) 6, 142, 265, 390, 1397, 1659, 1817
Sau3AI GATC 6 cut(s) 360, 1300, 1420, 1443, 1753, 2090
Sau96I GGNCC 5 cut(s) 108, 306, 423, 448, 1183
ScaI AGTACT 1 cut(s) 1349
SchI GAGTC 2 cut(s) 512, 1588
ScrFI CCNGG 2 cut(s) 22, 500
SduI GDGCHC 4 cut(s) 1097, 1455, 1619, 1684
SfaNI GCATC 4 cut(s) 106, 276, 1596, 1803
SfcI CTRYAG 3 cut(s) 1456, 1916, 2229
Sfr274I CTCGAG 1 cut(s) 443
SinI GGWCC 4 cut(s) 108, 423, 448, 1183
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 3 cut(s) 1109, 1125, 1166
SmlI CTYRAG 4 cut(s) 290, 356, 443, 587
SmoI CTYRAG 4 cut(s) 290, 356, 443, 587
SpeI ACTAGT 2 cut(s) 602, 959
Sse9I AATT 2 cut(s) 53, 984
SsiI CCGC 7 cut(s) 6, 70, 150, 262, 265, 390, 1659
SspI AATATT 3 cut(s) 676, 1738, 1902
SspMI CTAG 7 cut(s) 393, 603, 960, 1031, 1493, 1598, 2075
SstI GAGCTC 1 cut(s) 1619
StyD4I CCNGG 2 cut(s) 20, 498
StyI CCWWGG 3 cut(s) 136, 159, 705
TaaI ACNGT 4 cut(s) 40, 247, 747, 1457
TaiI ACGT 3 cut(s) 671, 1094, 1100
TaqI TCGA 5 cut(s) 410, 444, 578, 1835, 1990
TasI AATT 2 cut(s) 53, 984
TatI WGTACW 3 cut(s) 1310, 1347, 2007
TauI GCSGC 4 cut(s) 8, 267, 392, 1661
TfiI GAWTC 6 cut(s) 311, 379, 868, 1193, 1541, 1688
TscAI CASTG 3 cut(s) 1460, 1780, 2238
TseFI GTSAC 1 cut(s) 741
TseI GCWGC 3 cut(s) 141, 1396, 1816
Tsp45I GTSAC 1 cut(s) 741
TspDTI ATGAA 8 cut(s) 337, 542, 642, 713, 713, 996, 1398, 2028
TspGWI ACGGA 1 cut(s) 1842
TspRI CASTG 3 cut(s) 1460, 1780, 2238
Tth111I GACNNNGTC 1 cut(s) 2108
Van91I CCANNNNNTGG 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 587
VneI GTGCAC 1 cut(s) 1093
VpaK11BI GGWCC 4 cut(s) 108, 423, 448, 1183
XbaI TCTAGA 1 cut(s) 1597
XceI RCATGY 1 cut(s) 1896
XhoI CTCGAG 1 cut(s) 443
XmnI GAANNNNTTC 1 cut(s) 1157
XspI CTAG 7 cut(s) 393, 603, 960, 1031, 1493, 1598, 2075
ZrmI AGTACT 1 cut(s) 1349
Zsp2I ATGCAT 2 cut(s) 1116, 1677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.