pycom12652g00050

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012652
OG0005468
Physical Location & Seq
Reverse (-)
45573 .. 48204
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12652g00050.1

Sequence Viewer

Length: 2229 bp
ATGGCCGCCAATCCCATTTCTCAGAGACGAGCATTGGCACTCCTTAGACAGCAATTCTCCAGCCCTATCCTCAACTCTCAGCCAGCTTCTCTTCTCAGGTGCCGTTCCTCTCAGAAAACCCAGAACCACAAACACGATGAACAACTGCAGAACAATACCCAACGACCCAAATTGTCAACCCCCAAAACCAAAAGGGCTAAGGATATGGCCAAGCTTATCGACTCCATGCCGTGGTCAACTGAGCTCGAATCTTCTCTCTCTACAATCGCTTCCTCTCTCTCCAAAACGACAGTGCACCAAACTCTCCACCTTATCAAAGCCCCCAGCAAAGCCCTTCAATTTTTCAAGTGGGCGGAGGTAATGGGTTTCTCTCACAATGACCAATCCTACTTCTTGATGCTTGAAATCTTGGGTCACAATCGAAATCTCAATGCTGCTCGAAATTTCTTGTTTTCGATTGAAAAGAAGTCTAATGGGGCGGTTAAGCTCGAGGACCGGTTCTTTAATAGCTTGATAAGGAACTACGGTCGAGCTGGGCTGTTCCAAGAGTCTATAAAGCTTTTTAGTACAATGAAGTCGATTGGTATTTCGCCTTCTGTTGTTTCGTTCAATAGTCTGCTGTCGATTTTGCTTAGAAAGGGGCGGACCAATATGGCCAAGAATGTGTATGATGAAATGGTTAGTATGTATGGAGCTACACCGGATACTTGTACGTTTAATACATTGATTAGAGGGTTTTGTATGAACTCCATGGTTGATGAAGGTTTTCGCTTTTTCAAAGAAATGTCACGTTTCAAATGTGATCCGGATGTCATTACCTATAATACTCTTGTTGATGGGTTGTGTAGAGCAGGGAAGGTTGGTATTGCCCGTAATGTGGTGAAGGGCATGAATAAGAGAAGTGCAGATTTGAATCCAAATATTGTTACTTATACGACCTTGATTAGAGGTTATTGTATGAAACAAGAGATTGATGAGGCTTTATGTGTTTTGGAGGAGATGACTAGCCAAGGGTTGAAACCAAATGGAATTACCTGCAATACTCTAATTAAGGGGCTTTGTGAAGCGCATAAGCTAGATAAGATAAAAGACGTTTTGGAAGGAACGATGAGCGGTGGAGAATTTAGTCCTGATACTTGTACGTTTAACACACTGATGCATTCGCATTGCAATGCAGGAAACTTGGATGAAGCATTGAAGGTTTTTGCAAAGATGTCAGAGCTAAAGGTCCCACCAGATTCAGCTACATACAGTGTTTTGATATGCAGTTTGTGTCAACGAGGGGATTACAGTAGAGCAGAGGAGTTGTTCGATGAGCTATCAAAGAAAGAAATACTGTTAAGTGATGACGGTAGTAAGCCTCTTGTTGCCTCTTATAACCCTATTTTTGAGTATTTGTGTAGTAAGGGGAAAATGAAAAAGGCCGAGGCAGTGTTTAGGCAGCTAATGAGAAGAGGAACGCAAGACCCTGTGTCCTATAAGACTTTGATCATGGGCCATTGTAAGGAAGGTACATTTGAAGCCGGGTATGAACTCTTAGTCTGGATGCTTAGAAGGGATTTTGTGCCTGATGTCGAAATTTACGAATCCTTGATTGGTGGTCTCTTGCAGAAGGGTAAGGCCCTTCTTGCTCAACAGATGCTAGAGAAGATGCTGAAAAGCTCTCATCTCCCAAAAACGTCTACATTCCATTGTATACTTGCAGAACTTTTAGAAAAGAATTGTGCCCTTGAATCTGCCAATTGTGTTATTTTAATGCTGGAGAGAAAAATTAGACAAAATATTAATCTCTCAACACATCTTGTAAGGCTACTTTTCAGCAGTGGGTTGCGTGACAAAGCATTTGAGATTGTAGGGATGCTCCATGAGAATGGATACTCAATTAAAATGGAAGAAGTGGTTGATTTTCTTTGCCGGAGAAGAAAGCTGTTAGACGCATGCAAAATGTTGCAATTCAGTCTGCAGAAGCATCAAAGTGTCAGTATTGATATTTTCAACCAAGTCATCGAAGGGCTTTGTAATATCAATAAACCTTCAGAAGCATTCGGCTTGTACTATGAACTGGTTGAGAATGCAGGCTATCAGCAATTTCCGTGCTTAGGATCCTTAAAATCTGCACTGGAGATTGCAGGAAAATCAGTTGAAGCTGAGTTTCTATCTAAAAGGATGCCAGGAGAGCAGTCGCTGGACAAATCCCAGAGGTTGAGACCTCAACTAGGAGCATGCTGA

Protein Analysis

743

Amino Acids

83.52

Weight (kDa)

8.95

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 168 - 207 2.9e-07 PPR repeat family
PPR_3 PF13812 186 - 244 3.8e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 198 - 247 9.1e-13 PPR repeat family
PPR_1 PF12854 231 - 263 1.4e-10 PPR repeat
PPR_2 PF13041 234 - 283 2.2e-17 PPR repeat family
PPR PF01535 238 - 267 1.2e-06 PPR repeat
PPR_long PF17177 249 - 367 7.6e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 259 - 316 2.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 267 - 297 4.2e-11 PPR repeat
PPR_2 PF13041 269 - 303 6.1e-06 PPR repeat family
TPR_24 PF23276 283 - 404 3.7e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 302 - 351 1.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 304 - 335 4.8e-10 PPR repeat
PPR_2 PF13041 306 - 354 4.5e-17 PPR repeat family
PPR PF01535 309 - 339 9e-07 PPR repeat
PPR_3 PF13812 330 - 386 2.3e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 374 - 421 1.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 375 - 406 5.4e-11 PPR repeat
PPR_2 PF13041 377 - 426 1.5e-16 PPR repeat family
PPR PF01535 381 - 408 1.2e-06 PPR repeat
PPR_1 PF12854 410 - 439 1.6e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1377
Acc36I ACCTGC 1 cut(s) 1043
AccB1I GGYRCC 1 cut(s) 99
AccB7I CCANNNNNTGG 1 cut(s) 231
AccBSI CCGCTC 1 cut(s) 1113
AccI GTMKAC 2 cut(s) 1682, 1696
AccIII TCCGGA 1 cut(s) 805
AciI CCGC 5 cut(s) 6, 353, 479, 643, 1113
AclWI GGATC 3 cut(s) 797, 2097, 2110
AcoI YGGCCR 3 cut(s) 3, 207, 654
AcsI RAATTY 3 cut(s) 442, 1121, 1578
AcuI CTGAAG 1 cut(s) 2019
AfaI GTAC 5 cut(s) 568, 712, 1141, 1513, 2054
AfiI CCNNNNNNNGG 5 cut(s) 231, 877, 1504, 2099, 2216
AgeI ACCGGT 1 cut(s) 495
AhdI GACNNNNNGTC 1 cut(s) 1471
AjnI CCWGG 1 cut(s) 2170
AjuI GAANNNNNNNTTGG 2 cut(s) 1578, 1610
Alw21I GWGCWC 2 cut(s) 246, 297
Alw26I GTCTC 3 cut(s) 19, 1607, 2200
Alw44I GTGCAC 1 cut(s) 293
AlwI GGATC 3 cut(s) 797, 2097, 2110
AlwNI CAGNNNCTG 1 cut(s) 2185
Ama87I CYCGRG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 805
AoxI GGCC 6 cut(s) 3, 207, 654, 1422, 1495, 1620
ApaLI GTGCAC 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 434, 1441
ApoI RAATTY 3 cut(s) 442, 1121, 1578
ArsI GACNNNNNNTTYG 2 cut(s) 1826, 1858
AseI ATTAAT 1 cut(s) 1785
AsiGI ACCGGT 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 765
AspLEI GCGC 1 cut(s) 1069
AspS9I GGNCC 5 cut(s) 493, 645, 1228, 1495, 1621
AsuC2I CCSGG 1 cut(s) 1525
AsuHPI GGTGA 1 cut(s) 892
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 3 cut(s) 493, 645, 1228
BaeGI GKGCMC 2 cut(s) 297, 1729
BalI TGGCCA 2 cut(s) 209, 656
BamHI GGATCC 1 cut(s) 2102
BanI GGYRCC 1 cut(s) 99
BanII GRGCYC 1 cut(s) 246
BarI GAAGNNNNNNTAC 2 cut(s) 577, 609
Bbv12I GWGCWC 2 cut(s) 246, 297
BbvI GCAGC 2 cut(s) 421, 1453
BccI CCATC 1 cut(s) 830
BceAI ACGGC 2 cut(s) 87, 214
BciT130I CCWGG 1 cut(s) 2172
BciVI GTATCC 2 cut(s) 697, 1868
BclI TGATCA 1 cut(s) 1488
BcnI CCSGG 1 cut(s) 1525
BcoDI GTCTC 3 cut(s) 19, 1607, 2200
BfaI CTAG 4 cut(s) 1005, 1076, 1643, 2216
BfmI CTRYAG 2 cut(s) 146, 1961
BfuAI ACCTGC 1 cut(s) 1043
BfuI GTATCC 2 cut(s) 697, 1868
BisI GCNGC 3 cut(s) 6, 435, 1442
BlsI GCNGC 3 cut(s) 7, 436, 1443
Bme1390I CCNGG 2 cut(s) 1525, 2172
Bme18I GGWCC 3 cut(s) 493, 645, 1228
BmeRI GACNNNNNGTC 1 cut(s) 1471
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 5 cut(s) 493, 645, 1228, 1495, 1621
BmiI GGNNCC 3 cut(s) 101, 1230, 2104
BmrFI CCNGG 2 cut(s) 1525, 2172
BmsI GCATC 8 cut(s) 387, 1146, 1536, 1629, 1641, 1848, 1978, 2157
BpmI CTGGAG 3 cut(s) 43, 1781, 2141
Bpu10I CCTNAGC 2 cut(s) 198, 2098
BpuMI CCSGG 1 cut(s) 1525
BsaBI GATNNNNATC 1 cut(s) 912
BsaI GGTCTC 2 cut(s) 1607, 2200
BsaJI CCNNGG 4 cut(s) 230, 750, 1009, 1425
BsaWI WCCGGW 3 cut(s) 495, 700, 805
Bsc4I CCNNNNNNNGG 5 cut(s) 231, 877, 1504, 2099, 2216
Bse118I RCCGGY 1 cut(s) 495
Bse1I ACTGG 2 cut(s) 2067, 2124
Bse3DI GCAATG 2 cut(s) 1165, 1177
Bse8I GATNNNNATC 1 cut(s) 912
BseAI TCCGGA 1 cut(s) 805
BseBI CCWGG 1 cut(s) 2172
BseDI CCNNGG 4 cut(s) 230, 750, 1009, 1425
BseGI GGATG 5 cut(s) 814, 1192, 1551, 1863, 2172
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 5 cut(s) 231, 877, 1504, 2099, 2216
BseMI GCAATG 2 cut(s) 1165, 1177
BseMII CTCAG 6 cut(s) 35, 92, 109, 125, 231, 2139
BseNI ACTGG 2 cut(s) 2067, 2124
BseRI GAGGAG 2 cut(s) 1010, 1316
BseSI GKGCMC 2 cut(s) 297, 1729
BseXI GCAGC 2 cut(s) 421, 1453
BseYI CCCAGC 2 cut(s) 323, 533
BsgI GTGCAG 2 cut(s) 924, 2100
Bsh1285I CGRYCG 1 cut(s) 529
BshFI GGCC 6 cut(s) 5, 209, 656, 1424, 1497, 1622
BshNI GGYRCC 1 cut(s) 99
BshTI ACCGGT 1 cut(s) 495
BsiEI CGRYCG 1 cut(s) 529
BsiHKAI GWGCWC 2 cut(s) 246, 297
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 5 cut(s) 496, 701, 806, 1524, 1915
BslFI GGGAC 1 cut(s) 1214
BslI CCNNNNNNNGG 5 cut(s) 231, 877, 1504, 2099, 2216
BsmAI GTCTC 3 cut(s) 19, 1607, 2200
BsmBI CGTCTC 1 cut(s) 19
BsmFI GGGAC 1 cut(s) 1214
BsmI GAATGC 3 cut(s) 1159, 2042, 2077
BsnI GGCC 6 cut(s) 5, 209, 656, 1424, 1497, 1622
Bso31I GGTCTC 2 cut(s) 1607, 2200
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 246, 297, 1729
Bsp13I TCCGGA 1 cut(s) 805
Bsp143I GATC 3 cut(s) 802, 1488, 2102
Bsp19I CCATGG 1 cut(s) 750
BspACI CCGC 5 cut(s) 6, 353, 479, 643, 1113
BspANI GGCC 6 cut(s) 5, 209, 656, 1424, 1497, 1622
BspCNI CTCAG 6 cut(s) 34, 91, 108, 124, 232, 2140
BspEI TCCGGA 1 cut(s) 805
BspLI GGNNCC 3 cut(s) 101, 1230, 2104
BspMAI CTGCAG 2 cut(s) 150, 1965
BspMI ACCTGC 1 cut(s) 1043
BspPI GGATC 3 cut(s) 797, 2097, 2110
BspT107I GGYRCC 1 cut(s) 99
BspTNI GGTCTC 2 cut(s) 1607, 2200
BsrBI CCGCTC 1 cut(s) 1113
BsrDI GCAATG 2 cut(s) 1165, 1177
BsrFI RCCGGY 1 cut(s) 495
BsrI ACTGG 2 cut(s) 2067, 2124
BssAI RCCGGY 1 cut(s) 495
BssECI CCNNGG 4 cut(s) 230, 750, 1009, 1425
BssMI GATC 3 cut(s) 802, 1488, 2102
BssNAI GTATAC 1 cut(s) 1697
BssT1I CCWWGG 2 cut(s) 750, 1009
Bst1107I GTATAC 1 cut(s) 1697
Bst2UI CCWGG 1 cut(s) 2172
Bst4CI ACNGT 6 cut(s) 292, 527, 1253, 1292, 1338, 1352
Bst6I CTCTTC 2 cut(s) 96, 1447
BstC8I GCNNGC 4 cut(s) 84, 1939, 2077, 2224
BstDSI CCRYGG 2 cut(s) 230, 750
BstENI CCTNNNNNAGG 1 cut(s) 2214
BstF5I GGATG 5 cut(s) 814, 1192, 1551, 1863, 2172
BstHHI GCGC 1 cut(s) 1069
BstKTI GATC 3 cut(s) 805, 1491, 2105
BstMAI GTCTC 3 cut(s) 19, 1607, 2200
BstMBI GATC 3 cut(s) 802, 1488, 2102
BstMCI CGRYCG 1 cut(s) 529
BstMWI GCNNNNNNNGC 2 cut(s) 1628, 2176
BstNI CCWGG 1 cut(s) 2172
BstNSI RCATGY 2 cut(s) 1941, 2226
BstSCI CCNGG 2 cut(s) 1523, 2170
BstSFI CTRYAG 2 cut(s) 146, 1961
BstSLI GKGCMC 2 cut(s) 297, 1729
BstV1I GCAGC 2 cut(s) 421, 1453
BstX2I RGATCY 1 cut(s) 2102
BstXI CCANNNNNNTGG 1 cut(s) 1871
BstYI RGATCY 1 cut(s) 2102
BstZ17I GTATAC 1 cut(s) 1697
BsuI GTATCC 2 cut(s) 697, 1868
BsuRI GGCC 6 cut(s) 5, 209, 656, 1424, 1497, 1622
BtgI CCRYGG 2 cut(s) 230, 750
BtsCI GGATG 5 cut(s) 814, 1192, 1551, 1863, 2172
BtsI GCAGTG 2 cut(s) 1437, 1828
BtsIMutI CAGTG 6 cut(s) 297, 1151, 1258, 1437, 1828, 2117
BveI ACCTGC 1 cut(s) 1043
Cac8I GCNNGC 4 cut(s) 84, 1939, 2077, 2224
CaiI CAGNNNCTG 1 cut(s) 2185
CfoI GCGC 1 cut(s) 1069
Cfr10I RCCGGY 1 cut(s) 495
Cfr13I GGNCC 5 cut(s) 493, 645, 1228, 1495, 1621
CseI GACGC 1 cut(s) 1943
Csp6I GTAC 5 cut(s) 567, 711, 1140, 1512, 2053
CspAI ACCGGT 1 cut(s) 495
CviAII CATG 7 cut(s) 226, 751, 889, 1492, 1865, 1938, 2223
CviQI GTAC 5 cut(s) 567, 711, 1140, 1512, 2053
DpnI GATC 3 cut(s) 804, 1490, 2104
DpnII GATC 3 cut(s) 802, 1488, 2102
DriI GACNNNNNGTC 1 cut(s) 1471
EaeI YGGCCR 3 cut(s) 3, 207, 654
Eam1104I CTCTTC 2 cut(s) 96, 1447
Eam1105I GACNNNNNGTC 1 cut(s) 1471
EarI CTCTTC 2 cut(s) 96, 1447
EciI GGCGGA 2 cut(s) 368, 658
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 750, 1009
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 2 cut(s) 1607, 2200
Eco47I GGWCC 3 cut(s) 493, 645, 1228
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 2019
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 2214
EcoO109I RGGNCCY 2 cut(s) 1228, 1621
EcoRII CCWGG 1 cut(s) 2170
EcoT14I CCWWGG 2 cut(s) 750, 1009
EcoT22I ATGCAT 1 cut(s) 1161
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 2 cut(s) 750, 1009
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 7 cut(s) 229, 754, 892, 1495, 1868, 1941, 2226
FaqI GGGAC 1 cut(s) 1214
FatI CATG 7 cut(s) 225, 750, 888, 1491, 1864, 1937, 2222
FbaI TGATCA 1 cut(s) 1488
FblI GTMKAC 2 cut(s) 1682, 1696
Fnu4HI GCNGC 3 cut(s) 6, 435, 1442
FokI GGATG 5 cut(s) 821, 1199, 1558, 1870, 2179
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 6, 435, 1442
FspBI CTAG 4 cut(s) 1005, 1076, 1643, 2216
GlaI GCGC 1 cut(s) 1068
GluI GCNGC 3 cut(s) 6, 435, 1442
GsaI CCCAGC 2 cut(s) 327, 537
GsuI CTGGAG 3 cut(s) 43, 1781, 2141
HaeIII GGCC 6 cut(s) 5, 209, 656, 1424, 1497, 1622
HapII CCGG 5 cut(s) 496, 701, 806, 1524, 1915
HgaI GACGC 1 cut(s) 1943
HhaI GCGC 1 cut(s) 1069
Hin1II CATG 7 cut(s) 229, 754, 892, 1495, 1868, 1941, 2226
Hin6I GCGC 1 cut(s) 1067
HinP1I GCGC 1 cut(s) 1067
HincII GTYRAC 3 cut(s) 177, 237, 1277
HindII GTYRAC 3 cut(s) 177, 237, 1277
HindIII AAGCTT 2 cut(s) 212, 557
HinfI GANTC 7 cut(s) 221, 248, 548, 913, 1238, 1586, 1733
HpaII CCGG 5 cut(s) 496, 701, 806, 1524, 1915
HphI GGTGA 1 cut(s) 892
Hpy166II GTNNAC 6 cut(s) 177, 237, 295, 1277, 1683, 1697
Hpy188I TCNGA 4 cut(s) 24, 114, 1219, 2038
Hpy188III TCNNGA 4 cut(s) 394, 806, 1130, 1543
Hpy8I GTNNAC 6 cut(s) 177, 237, 295, 1277, 1683, 1697
HpyCH4III ACNGT 6 cut(s) 292, 527, 1253, 1292, 1338, 1352
HpyCH4IV ACGT 5 cut(s) 713, 790, 1092, 1142, 1679
HpyF10VI GCNNNNNNNGC 2 cut(s) 1628, 2176
HpySE526I ACGT 5 cut(s) 713, 790, 1092, 1142, 1679
Hsp92II CATG 7 cut(s) 229, 754, 892, 1495, 1868, 1941, 2226
HspAI GCGC 1 cut(s) 1067
Kpn2I TCCGGA 1 cut(s) 805
Ksp22I TGATCA 1 cut(s) 1488
Kzo9I GATC 3 cut(s) 802, 1488, 2102
LmnI GCTCC 3 cut(s) 692, 1866, 2219
Lsp1109I GCAGC 2 cut(s) 421, 1453
LweI GCATC 8 cut(s) 387, 1146, 1536, 1629, 1641, 1848, 1978, 2157
MaeI CTAG 4 cut(s) 1005, 1076, 1643, 2216
MaeII ACGT 5 cut(s) 713, 790, 1092, 1142, 1679
MaeIII GTNAC 4 cut(s) 413, 786, 925, 1832
MalI GATC 3 cut(s) 804, 1490, 2104
MbiI CCGCTC 1 cut(s) 1113
MboI GATC 3 cut(s) 802, 1488, 2102
MboII GAAGA 6 cut(s) 83, 243, 1464, 1660, 1904, 1932
MfeI CAATTG 1 cut(s) 1741
MflI RGATCY 1 cut(s) 2102
MhlI GDGCHC 3 cut(s) 246, 297, 1729
MlsI TGGCCA 2 cut(s) 209, 656
MluNI TGGCCA 2 cut(s) 209, 656
MlyI GAGTC 2 cut(s) 215, 557
Mox20I TGGCCA 2 cut(s) 209, 656
Mph1103I ATGCAT 1 cut(s) 1161
MroI TCCGGA 1 cut(s) 805
MroXI GAANNNNTTC 1 cut(s) 765
MscI TGGCCA 2 cut(s) 209, 656
MslI CAYNNNNRTG 4 cut(s) 1154, 1170, 1869, 1974
Msp20I TGGCCA 2 cut(s) 209, 656
MspI CCGG 5 cut(s) 496, 701, 806, 1524, 1915
MspR9I CCNGG 2 cut(s) 1525, 2172
MunI CAATTG 1 cut(s) 1741
Mva1269I GAATGC 3 cut(s) 1159, 2042, 2077
MvaI CCWGG 1 cut(s) 2172
MwoI GCNNNNNNNGC 2 cut(s) 1628, 2176
NciI CCSGG 1 cut(s) 1525
NcoI CCATGG 1 cut(s) 750
NdeII GATC 3 cut(s) 802, 1488, 2102
NlaIII CATG 7 cut(s) 229, 754, 892, 1495, 1868, 1941, 2226
NlaIV GGNNCC 3 cut(s) 101, 1230, 2104
NmeAIII GCCGAG 1 cut(s) 1450
NmuCI GTSAC 3 cut(s) 413, 786, 1832
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 2 cut(s) 1941, 2226
PaeI GCATGC 2 cut(s) 1941, 2226
PaeR7I CTCGAG 1 cut(s) 488
PcsI WCGNNNNNNNCGW 1 cut(s) 1581
PctI GAATGC 3 cut(s) 1159, 2042, 2077
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 5 cut(s) 248, 913, 1238, 1586, 1733
PflMI CCANNNNNTGG 1 cut(s) 231
PinAI ACCGGT 1 cut(s) 495
PkrI GCNGC 3 cut(s) 7, 436, 1443
PleI GAGTC 2 cut(s) 215, 556
PpsI GAGTC 2 cut(s) 215, 556
PpuMI RGGWCCY 1 cut(s) 1228
PshBI ATTAAT 1 cut(s) 1785
PsiI TTATAA 1 cut(s) 1377
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 1228
Psp6I CCWGG 1 cut(s) 2170
PspFI CCCAGC 2 cut(s) 323, 533
PspGI CCWGG 1 cut(s) 2170
PspN4I GGNNCC 3 cut(s) 101, 1230, 2104
PspPI GGNCC 5 cut(s) 493, 645, 1228, 1495, 1621
PspPPI RGGWCCY 1 cut(s) 1228
PspXI VCTCGAGB 1 cut(s) 488
PstI CTGCAG 2 cut(s) 150, 1965
PstNI CAGNNNCTG 1 cut(s) 2185
PsuI RGATCY 1 cut(s) 2102
RsaI GTAC 5 cut(s) 568, 712, 1141, 1513, 2054
RsaNI GTAC 5 cut(s) 567, 711, 1140, 1512, 2053
RseI CAYNNNNRTG 4 cut(s) 1154, 1170, 1869, 1974
SacI GAGCTC 1 cut(s) 246
SatI GCNGC 3 cut(s) 6, 435, 1442
Sau3AI GATC 3 cut(s) 802, 1488, 2102
Sau96I GGNCC 5 cut(s) 493, 645, 1228, 1495, 1621
SchI GAGTC 2 cut(s) 215, 557
ScrFI CCNGG 2 cut(s) 1525, 2172
SduI GDGCHC 3 cut(s) 246, 297, 1729
SfaNI GCATC 8 cut(s) 387, 1146, 1536, 1629, 1641, 1848, 1978, 2157
SfcI CTRYAG 2 cut(s) 146, 1961
Sfr274I CTCGAG 1 cut(s) 488
SinI GGWCC 3 cut(s) 493, 645, 1228
SlaI CTCGAG 1 cut(s) 488
SmiMI CAYNNNNRTG 4 cut(s) 1154, 1170, 1869, 1974
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
SphI GCATGC 2 cut(s) 1941, 2226
SsiI CCGC 5 cut(s) 6, 353, 479, 643, 1113
SspI AATATT 2 cut(s) 922, 1783
SspMI CTAG 4 cut(s) 1005, 1076, 1643, 2216
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 1523, 2170
StyI CCWWGG 2 cut(s) 750, 1009
TaaI ACNGT 6 cut(s) 292, 527, 1253, 1292, 1338, 1352
TaiI ACGT 5 cut(s) 716, 793, 1095, 1145, 1682
TatI WGTACW 2 cut(s) 566, 2052
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 5 cut(s) 248, 913, 1238, 1586, 1733
TscAI CASTG 6 cut(s) 297, 1158, 1258, 1437, 1828, 2124
TseFI GTSAC 3 cut(s) 413, 786, 1832
TseI GCWGC 2 cut(s) 434, 1441
Tsp45I GTSAC 3 cut(s) 413, 786, 1832
TspGWI ACGGA 1 cut(s) 2082
TspRI CASTG 6 cut(s) 297, 1158, 1258, 1437, 1828, 2124
Van91I CCANNNNNTGG 1 cut(s) 231
VneI GTGCAC 1 cut(s) 293
VpaK11BI GGWCC 3 cut(s) 493, 645, 1228
VspI ATTAAT 1 cut(s) 1785
XagI CCTNNNNNAGG 1 cut(s) 2214
XapI RAATTY 3 cut(s) 442, 1121, 1578
XceI RCATGY 2 cut(s) 1941, 2226
XhoI CTCGAG 1 cut(s) 488
XmiI GTMKAC 2 cut(s) 1682, 1696
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 4 cut(s) 1005, 1076, 1643, 2216
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.