Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0005468

Physical Location & Seq

Genomic Coordinates Reverse (-)

30456661 .. 30462495

Gene Structure 5835 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00308800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2154 bp

ATGGCCGTCAAACCCATTTCCCGGCGACCAGCCTTGACAGTTCTCCGAGAGCAATTCCTCAGACCCAACCGCAACTCCAAACCTTCTTCTCCTCCTCTCAGGTCCTCCGATGCCGAAAACCCCAAATCCCAAGGCAGCAAAACCGCCAAAGCCATGGCGTCGCTCATCAACTCCAAGCCATGGTCATCTCGCCTCCAGTCTTCTCTCTCTTCTCTCTCTCCCTCCATCTCCAACACCACCGTCTCCCAGACCCTCCGCCGCATCAAAACCCCAACCCAGGCCCTCAAATTCTTCAACTGGGCCGAAGCAATGGGCTTCTCCCACAACGCCCAATCCTTCTTCATGTTGCTTGAGATTTTCGGCCGGAACCGGAATCTCAATGCCGCTAGAAATCTCTTGTTTTCGATCGAGAAGAGGTCCAATGGGAAGGTTAAGCTCGAGGACCGGTTTTTTAATAGCTTGATTAGGAGCTATGGGGACGCAGGGTTGTTTCAGGAGTCTGTGAAGCTTTTTGGGACGATGAAGTCTATGGGGATATCGGCTTCTGTTGTTACTTTCAATAGTTTGCTTTCGATTTTGCTTAAGAGGGGTAGGACTAGTATGGCTAAGAATGTTTATGATGAAATGCTTGGAATGTATGGAGTTACGCCGGATACGCATACGTTTAACATTTTGATTAGGGGGTTCTGTATGAACTCCATGGTTGATGAGGGGTTTCATTATTTTAAGGAAATGTCAAGGTTGAAATGTGAGCCGGATGTGGTTACTTATAATACGATTGTTGATGGGTTGTGTAGAGCAGGGAAGGTTGAGATTGCGCGTAATGTGGTTAAGGGTATGAGGAAGAGAAGTGGGGAGTTGAATCCGAATGTTGTTACTTATACCACATTGATTAGAGGGTATTGTGTGAAAGAAGATGTTGATGAGGCTTTGTGTGTTTTGGAGGAGATGACTAGTCAAGGGTTGAAACCAAATGAGATTACCTATAATACTCTGCTTAAAGGGCTTTGTGAAGCAAAGAAGCTAGATAAGGTAAAAGAAGTCTTGGAGGGAACAATGAGAGGTGAGGGATTTACTCCTGATACGTTTACGTTCAACATGCTGATGCATTCGCATTGCAATGCAGGAAACTTAGAGGAAGCATTGAAGGTTTTCACAATGATGTCAGAGTTACAGGTCCCTCCAGATTCAGCTACATACAGCGTTTTGATACGCAGTTTGTGTCAGCGAAGAGATTATAGTAAAGCTGAGGAGTTGTTTGATGAGCTATCCAAGAAGCAAATCTTGTTAAGTGATCGTGGCTGTACACCTGTTGTTGCCTCATACAAACCTATCTTTGAGTACTTATGTAGTAATGGGAGAACTAAGAAAGCAGATGAAGTGTTTAGGCAGCTACTGAAAAGAGGAACACAAGATCCTCCATCCTACAAGACTTTGATCATGGGCCACTGTAGGGAAGGCACATTTGAAGCTGGGTACAAACTCCTAGTCTTGATGCTTAGAAGGGATTTTGTTCCTGATGTCGAGATTTATGATTCCTTGATTGATGGTCTGTTGGAAAAGGGTAAGCCCCTTCTTGCACAACAGACTCTAGAGAAGATGCTGAAGAGCTCTCATCTCCCAAAAACATCTACTTTCCATTCTATACTGGCAGCTCTTTTAGAAATGCATTCTGCCCGTGAATCTGCAAGCTTTGTTATTTTAATGTTGGAGAATAACATTAGACAAAATATTAACCTCTCAACAGATCTTATAAGGCTTCTTTTCAGTGAGGGATTGCGGGATAAAGCATTTGAGATTGTAGGGATGCTGTATGATAACGGATACTCGATTAAAATAGAAGAAGTGGTCAGTTTCCTTTACCAGAGCAGAAAGCTAATAGAGGCATGTGAAATATTGCGATTTACTCTGCGGAAGCAAGAAAATGTCAGTATTGATATATTTGACCAACTGATATTAGGGCTATGTAATATCAATAAGCTTCGAGAAGCATTTGGCTTGTACTATGAACTAATTGAGAATGGCGACCACCAGCAGTTGCTGTGCTTACATCATTTAAAGTCTTCCCTAGAGGTTGCAGGTAGATCAGTTGAAGCTGACTTTGTCTCTAAAAGAATGCCAAAACATCAACTGGTGGACAAATCTGGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Chal_sti_synt_C Chal_sti_synt_N PPR PPR_1 PPR_2 PPR_3

Protein Analysis

717

Amino Acids

81.14

Weight (kDa)

9.1

Isoelectric Point (pI)

45.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	151 - 190	6.8e-07	PPR repeat family
PPR_2	PF13041	182 - 230	2.4e-13	PPR repeat family
PPR_long	PF17177	202 - 352	8.3e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	214 - 246	3.8e-08	PPR repeat
PPR_2	PF13041	217 - 266	3.6e-16	PPR repeat family
PPR	PF01535	221 - 250	8.3e-06	PPR repeat
PPR_1	PF12854	250 - 281	2.1e-12	PPR repeat
PPR_2	PF13041	252 - 285	1.5e-07	PPR repeat family
TPR_24	PF23276	266 - 350	1.4e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	287 - 318	5.3e-11	PPR repeat
PPR_3	PF13812	287 - 334	7.3e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	289 - 337	1.6e-19	PPR repeat family
PPR	PF01535	292 - 322	1.1e-07	PPR repeat
PPR_1	PF12854	321 - 349	9.3e-08	PPR repeat
PPR_3	PF13812	353 - 405	4.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	357 - 388	5.3e-10	PPR repeat
PPR_2	PF13041	360 - 409	6e-16	PPR repeat family
PPR_1	PF12854	393 - 422	3.7e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005468)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02060
fragaria_vesca	FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470
malus_domestica	MD02G1218600.v1.1
prunus_persica	Prupe.2G122500_v2.0.a1
pyrus_communis	pycom12652g00050
rosa_chinensis	RchiOBHm_Chr1g0346171
rosa_laevigata	RLG00000028810
rosa_multiflora	Rmu_sc0003809.1_g000024
rosa_roxburghii	Rroxscaffold_4G00308800
rosa_rugosa	Rorug01G0178600
rosa_samantha	Rh1AG197700 Rh1BG162900 Rh1BG163000 Rh1BG163800 Rh1CG182800 Rh1DG193600 Rh1DG193700 Rh1DG194800
rosa_wichuraiana	Rw1G016310 Rw1G016590 Rw1G016620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	771, 1754
AasI	GACNNNNNNGTC	1 cut(s)	523
Acc36I	ACCTGC	1 cut(s)	2069
AccB7I	CCANNNNNTGG	1 cut(s)	180
AccII	CGCG	1 cut(s)	820
AciI	CCGC	7 cut(s)	70, 144, 256, 259, 384, 1780, 1912
AclWI	GGATC	1 cut(s)	1409
AcoI	YGGCCR	2 cut(s)	3, 361
AcsI	RAATTY	1 cut(s)	287
AcuI	CTGAAG	1 cut(s)	1625
AcyI	GRCGYC	1 cut(s)	158
AfaI	GTAC	4 cut(s)	1306, 1343, 1478, 2003
AfiI	CCNNNNNNNGG	4 cut(s)	21, 180, 277, 1453
AflII	CTTAAG	1 cut(s)	581
AgeI	ACCGGT	1 cut(s)	444
AgsI	TTSAA	9 cut(s)	295, 559, 745, 862, 967, 1096, 1147, 1469, 2093
AhlI	ACTAGT	2 cut(s)	596, 953
AjnI	CCWGG	1 cut(s)	276
AloI	GAACNNNNNNTCC	2 cut(s)	1399, 1431
Alw21I	GWGCWC	1 cut(s)	1613
Alw26I	GTCTC	2 cut(s)	247, 2110
AlwI	GGATC	1 cut(s)	1409
AlwNI	CAGNNNCTG	2 cut(s)	1396, 2041
Ama87I	CYCGRG	1 cut(s)	437
AoxI	GGCC	5 cut(s)	3, 279, 300, 361, 1444
ApeKI	GCWGC	3 cut(s)	135, 1390, 1652
ApoI	RAATTY	1 cut(s)	287
ArsI	GACNNNNNNTTYG	2 cut(s)	1473, 1505
AsiGI	ACCGGT	1 cut(s)	444
Asp700I	GAANNNNTTC	1 cut(s)	1151
AspLEI	GCGC	1 cut(s)	820
AspS9I	GGNCC	7 cut(s)	102, 280, 300, 417, 442, 1177, 1444
AsuC2I	CCSGG	1 cut(s)	22
AsuHPI	GGTGA	1 cut(s)	1076
AvaI	CYCGRG	1 cut(s)	437
AvaII	GGWCC	4 cut(s)	102, 417, 442, 1177
BanII	GRGCYC	1 cut(s)	1613
BbsI	GAAGAC	2 cut(s)	192, 2055
Bbv12I	GWGCWC	1 cut(s)	1613
BbvCI	CCTCAGC	1 cut(s)	1248
BbvI	GCAGC	3 cut(s)	147, 1402, 1664
BccI	CCATC	4 cut(s)	233, 779, 1429, 1541
BciT130I	CCWGG	1 cut(s)	278
BciVI	GTATCC	2 cut(s)	646, 1817
BclI	TGATCA	1 cut(s)	1437
BcnI	CCSGG	1 cut(s)	22
BcoDI	GTCTC	2 cut(s)	247, 2110
BcuI	ACTAGT	2 cut(s)	596, 953
BfaI	CTAG	7 cut(s)	387, 597, 954, 1025, 1487, 1592, 2069
BfmI	CTRYAG	1 cut(s)	1450
BfrI	CTTAAG	1 cut(s)	581
BfuAI	ACCTGC	1 cut(s)	2069
BfuI	GTATCC	2 cut(s)	646, 1817
BglII	AGATCT	1 cut(s)	1747
BisI	GCNGC	5 cut(s)	136, 259, 384, 1391, 1653
BlsI	GCNGC	5 cut(s)	137, 260, 385, 1392, 1654
BmcAI	AGTACT	1 cut(s)	1343
Bme1390I	CCNGG	2 cut(s)	22, 278
Bme18I	GGWCC	4 cut(s)	102, 417, 442, 1177
BmeT110I	CYCGRG	1 cut(s)	437
BmgT120I	GGNCC	7 cut(s)	102, 280, 300, 417, 442, 1177, 1444
BmiI	GGNNCC	2 cut(s)	368, 1179
BmrFI	CCNGG	2 cut(s)	22, 278
BmrI	ACTGGG	1 cut(s)	307
BmsI	GCATC	6 cut(s)	100, 270, 1095, 1485, 1590, 1797
BmuI	ACTGGG	1 cut(s)	307
BpiI	GAAGAC	2 cut(s)	192, 2055
BpmI	CTGGAG	2 cut(s)	179, 1167
Bpu10I	CCTNAGC	1 cut(s)	1248
BpuEI	CTTGAG	1 cut(s)	371
BpuMI	CCSGG	1 cut(s)	22
BsaHI	GRCGYC	1 cut(s)	158
BsaJI	CCNNGG	5 cut(s)	130, 153, 179, 276, 699
BsaWI	WCCGGW	2 cut(s)	369, 444
BsaXI	ACNNNNNCTCC	2 cut(s)	59, 89
Bsc4I	CCNNNNNNNGG	4 cut(s)	21, 180, 277, 1453
Bse118I	RCCGGY	1 cut(s)	444
Bse1I	ACTGG	4 cut(s)	196, 302, 1653, 2136
Bse3DI	GCAATG	3 cut(s)	315, 1114, 1126
BseBI	CCWGG	1 cut(s)	278
BseDI	CCNNGG	5 cut(s)	130, 153, 179, 276, 699
BseGI	GGATG	3 cut(s)	763, 1421, 1812
BseLI	CCNNNNNNNGG	4 cut(s)	21, 180, 277, 1453
BseMI	GCAATG	3 cut(s)	315, 1114, 1126
BseMII	CTCAG	3 cut(s)	73, 112, 1239
BseNI	ACTGG	4 cut(s)	196, 302, 1653, 2136
BseRI	GAGGAG	4 cut(s)	81, 84, 959, 1265
BseX3I	CGGCCG	1 cut(s)	361
BseXI	GCAGC	3 cut(s)	147, 1402, 1664
BseYI	CCCAGC	1 cut(s)	1472
Bsh1236I	CGCG	1 cut(s)	820
Bsh1285I	CGRYCG	2 cut(s)	364, 408
BshFI	GGCC	5 cut(s)	5, 281, 302, 363, 1446
BshTI	ACCGGT	1 cut(s)	444
BsiEI	CGRYCG	2 cut(s)	364, 408
BsiHKAI	GWGCWC	1 cut(s)	1613
BsiHKCI	CYCGRG	1 cut(s)	437
BsiSI	CCGG	6 cut(s)	22, 364, 370, 445, 650, 755
BslFI	GGGAC	3 cut(s)	491, 529, 1163
BslI	CCNNNNNNNGG	4 cut(s)	21, 180, 277, 1453
BsmAI	GTCTC	2 cut(s)	247, 2110
BsmBI	CGTCTC	1 cut(s)	247
BsmFI	GGGAC	3 cut(s)	491, 529, 1163
BsmI	GAATGC	3 cut(s)	1108, 1669, 2121
BsnI	GGCC	5 cut(s)	5, 281, 302, 363, 1446
BsoBI	CYCGRG	1 cut(s)	437
Bsp1286I	GDGCHC	1 cut(s)	1613
Bsp1407I	TGTACA	1 cut(s)	1304
Bsp143I	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
Bsp19I	CCATGG	3 cut(s)	153, 179, 699
BspACI	CCGC	7 cut(s)	70, 144, 256, 259, 384, 1780, 1912
BspANI	GGCC	5 cut(s)	5, 281, 302, 363, 1446
BspCNI	CTCAG	3 cut(s)	72, 111, 1240
BspFNI	CGCG	1 cut(s)	820
BspLI	GGNNCC	2 cut(s)	368, 1179
BspMI	ACCTGC	1 cut(s)	2069
BspPI	GGATC	1 cut(s)	1409
BspQI	GCTCTTC	1 cut(s)	1601
BspTI	CTTAAG	1 cut(s)	581
BsrDI	GCAATG	3 cut(s)	315, 1114, 1126
BsrFI	RCCGGY	1 cut(s)	444
BsrGI	TGTACA	1 cut(s)	1304
BsrI	ACTGG	4 cut(s)	196, 302, 1653, 2136
BssAI	RCCGGY	1 cut(s)	444
BssECI	CCNNGG	5 cut(s)	130, 153, 179, 276, 699
BssMI	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
BssNI	GRCGYC	1 cut(s)	158
BssT1I	CCWWGG	4 cut(s)	130, 153, 179, 699
Bst2UI	CCWGG	1 cut(s)	278
Bst4CI	ACNGT	3 cut(s)	40, 241, 1451
Bst6I	CTCTTC	5 cut(s)	214, 407, 839, 1225, 1601
BstACI	GRCGYC	1 cut(s)	158
BstAFI	CTTAAG	1 cut(s)	581
BstAUI	TGTACA	1 cut(s)	1304
BstC8I	GCNNGC	1 cut(s)	1690
BstDEI	CTNAG	7 cut(s)	59, 98, 606, 1132, 1248, 1365, 1499
BstDSI	CCRYGG	3 cut(s)	153, 179, 699
BstF5I	GGATG	3 cut(s)	763, 1421, 1812
BstFNI	CGCG	1 cut(s)	820
BstHHI	GCGC	1 cut(s)	820
BstKTI	GATC	6 cut(s)	408, 1297, 1417, 1440, 1750, 2087
BstMAI	GTCTC	2 cut(s)	247, 2110
BstMBI	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
BstMCI	CGRYCG	2 cut(s)	364, 408
BstMWI	GCNNNNNNNGC	2 cut(s)	655, 1003
BstNI	CCWGG	1 cut(s)	278
BstNSI	RCATGY	2 cut(s)	1102, 1890
BstSCI	CCNGG	2 cut(s)	20, 276
BstSFI	CTRYAG	1 cut(s)	1450
BstUI	CGCG	1 cut(s)	820
BstV1I	GCAGC	3 cut(s)	147, 1402, 1664
BstV2I	GAAGAC	2 cut(s)	192, 2055
BstX2I	RGATCY	2 cut(s)	1414, 1747
BstXI	CCANNNNNNTGG	1 cut(s)	154
BstYI	RGATCY	2 cut(s)	1414, 1747
BstZI	CGGCCG	1 cut(s)	361
BsuI	GTATCC	2 cut(s)	646, 1817
BsuRI	GGCC	5 cut(s)	5, 281, 302, 363, 1446
BtgI	CCRYGG	3 cut(s)	153, 179, 699
BtsCI	GGATG	3 cut(s)	763, 1421, 1812
BtsIMutI	CAGTG	2 cut(s)	1447, 1774
BveI	ACCTGC	1 cut(s)	2069
Cac8I	GCNNGC	1 cut(s)	1690
CaiI	CAGNNNCTG	2 cut(s)	1396, 2041
CfoI	GCGC	1 cut(s)	820
Cfr10I	RCCGGY	1 cut(s)	444
Cfr13I	GGNCC	7 cut(s)	102, 280, 300, 417, 442, 1177, 1444
CseI	GACGC	2 cut(s)	147, 488
Csp6I	GTAC	4 cut(s)	1305, 1342, 1477, 2002
CspAI	ACCGGT	1 cut(s)	444
CviAII	CATG	7 cut(s)	154, 180, 343, 700, 1099, 1441, 1887
CviQI	GTAC	4 cut(s)	1305, 1342, 1477, 2002
DdeI	CTNAG	7 cut(s)	59, 98, 606, 1132, 1248, 1365, 1499
DpnI	GATC	6 cut(s)	407, 1296, 1416, 1439, 1749, 2086
DpnII	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
DraI	TTTAAA	1 cut(s)	2058
DrdI	GACNNNNNNGTC	1 cut(s)	523
DseDI	GACNNNNNNGTC	1 cut(s)	523
EaeI	YGGCCR	2 cut(s)	3, 361
EagI	CGGCCG	1 cut(s)	361
Eam1104I	CTCTTC	5 cut(s)	214, 407, 839, 1225, 1601
EarI	CTCTTC	5 cut(s)	214, 407, 839, 1225, 1601
EciI	GGCGGA	1 cut(s)	245
Ecl136II	GAGCTC	1 cut(s)	1611
EclXI	CGGCCG	1 cut(s)	361
Eco130I	CCWWGG	4 cut(s)	130, 153, 179, 699
Eco24I	GRGCYC	1 cut(s)	1613
Eco32I	GATATC	1 cut(s)	537
Eco47I	GGWCC	4 cut(s)	102, 417, 442, 1177
Eco52I	CGGCCG	1 cut(s)	361
Eco53kI	GAGCTC	1 cut(s)	1611
Eco57I	CTGAAG	1 cut(s)	1625
Eco88I	CYCGRG	1 cut(s)	437
EcoICRI	GAGCTC	1 cut(s)	1611
EcoO109I	RGGNCCY	3 cut(s)	102, 280, 1177
EcoRII	CCWGG	1 cut(s)	276
EcoRV	GATATC	1 cut(s)	537
EcoT14I	CCWWGG	4 cut(s)	130, 153, 179, 699
EcoT22I	ATGCAT	2 cut(s)	1110, 1671
EcoT38I	GRGCYC	1 cut(s)	1613
ErhI	CCWWGG	4 cut(s)	130, 153, 179, 699
Esp3I	CGTCTC	1 cut(s)	247
FaeI	CATG	7 cut(s)	157, 183, 346, 703, 1102, 1444, 1890
FalI	AAGNNNNNCTT	2 cut(s)	1268, 1300
FaqI	GGGAC	3 cut(s)	491, 529, 1163
FatI	CATG	7 cut(s)	153, 179, 342, 699, 1098, 1440, 1886
FauI	CCCGC	1 cut(s)	1773
FbaI	TGATCA	1 cut(s)	1437
Fnu4HI	GCNGC	5 cut(s)	136, 259, 384, 1391, 1653
FokI	GGATG	3 cut(s)	770, 1408, 1819
FriOI	GRGCYC	1 cut(s)	1613
Fsp4HI	GCNGC	5 cut(s)	136, 259, 384, 1391, 1653
FspBI	CTAG	7 cut(s)	387, 597, 954, 1025, 1487, 1592, 2069
GlaI	GCGC	1 cut(s)	819
GluI	GCNGC	5 cut(s)	136, 259, 384, 1391, 1653
GsaI	CCCAGC	1 cut(s)	1476
GsuI	CTGGAG	2 cut(s)	179, 1167
HaeIII	GGCC	5 cut(s)	5, 281, 302, 363, 1446
HapII	CCGG	6 cut(s)	22, 364, 370, 445, 650, 755
HgaI	GACGC	2 cut(s)	147, 488
HhaI	GCGC	1 cut(s)	820
Hin1I	GRCGYC	1 cut(s)	158
Hin1II	CATG	7 cut(s)	157, 183, 346, 703, 1102, 1444, 1890
Hin6I	GCGC	1 cut(s)	818
HinP1I	GCGC	1 cut(s)	818
HindIII	AAGCTT	3 cut(s)	506, 1690, 1979
HinfI	GANTC	7 cut(s)	373, 497, 862, 1187, 1535, 1588, 1682
HpaII	CCGG	6 cut(s)	22, 364, 370, 445, 650, 755
HphI	GGTGA	1 cut(s)	1076
Hpy166II	GTNNAC	3 cut(s)	1089, 1307, 2137
Hpy188I	TCNGA	5 cut(s)	47, 62, 109, 867, 1168
Hpy188III	TCNNGA	9 cut(s)	409, 494, 1079, 1184, 1492, 1517, 1525, 1592, 1985
Hpy8I	GTNNAC	3 cut(s)	1089, 1307, 2137
Hpy99I	CGWCG	1 cut(s)	163
HpyAV	CCTTC	8 cut(s)	93, 346, 421, 799, 1141, 1451, 1497, 1583
HpyCH4III	ACNGT	3 cut(s)	40, 241, 1451
HpyCH4IV	ACGT	3 cut(s)	662, 1085, 1091
HpyCH4V	TGCA	7 cut(s)	1108, 1119, 1124, 1580, 1669, 1688, 2078
HpyF10VI	GCNNNNNNNGC	2 cut(s)	655, 1003
HpyF3I	CTNAG	7 cut(s)	59, 98, 606, 1132, 1248, 1365, 1499
HpySE526I	ACGT	3 cut(s)	662, 1085, 1091
Hsp92I	GRCGYC	1 cut(s)	158
Hsp92II	CATG	7 cut(s)	157, 183, 346, 703, 1102, 1444, 1890
HspAI	GCGC	1 cut(s)	818
Ksp22I	TGATCA	1 cut(s)	1437
Kzo9I	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
LguI	GCTCTTC	1 cut(s)	1601
LmnI	GCTCC	1 cut(s)	468
Lsp1109I	GCAGC	3 cut(s)	147, 1402, 1664
LweI	GCATC	6 cut(s)	100, 270, 1095, 1485, 1590, 1797
MaeI	CTAG	7 cut(s)	387, 597, 954, 1025, 1487, 1592, 2069
MaeII	ACGT	3 cut(s)	662, 1085, 1091
MaeIII	GTNAC	5 cut(s)	550, 643, 763, 874, 1170
MalI	GATC	6 cut(s)	407, 1296, 1416, 1439, 1749, 2086
MboI	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
MflI	RGATCY	2 cut(s)	1414, 1747
MhlI	GDGCHC	1 cut(s)	1613
MluCI	AATT	3 cut(s)	53, 287, 2013
MlyI	GAGTC	2 cut(s)	506, 1582
MmeI	TCCRAC	3 cut(s)	255, 1536, 1689
Mph1103I	ATGCAT	2 cut(s)	1110, 1671
MroXI	GAANNNNTTC	1 cut(s)	1151
MslI	CAYNNNNRTG	3 cut(s)	1103, 1119, 1160
MspCI	CTTAAG	1 cut(s)	581
MspI	CCGG	6 cut(s)	22, 364, 370, 445, 650, 755
MspR9I	CCNGG	2 cut(s)	22, 278
Mva1269I	GAATGC	3 cut(s)	1108, 1669, 2121
MvaI	CCWGG	1 cut(s)	278
MvnI	CGCG	1 cut(s)	820
MwoI	GCNNNNNNNGC	2 cut(s)	655, 1003
NciI	CCSGG	1 cut(s)	22
NcoI	CCATGG	3 cut(s)	153, 179, 699
NdeII	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
NlaIII	CATG	7 cut(s)	157, 183, 346, 703, 1102, 1444, 1890
NlaIV	GGNNCC	2 cut(s)	368, 1179
NsiI	ATGCAT	2 cut(s)	1110, 1671
NspI	RCATGY	2 cut(s)	1102, 1890
PaeR7I	CTCGAG	1 cut(s)	437
PciSI	GCTCTTC	1 cut(s)	1601
PctI	GAATGC	3 cut(s)	1108, 1669, 2121
PdmI	GAANNNNTTC	1 cut(s)	1151
PfeI	GAWTC	5 cut(s)	373, 862, 1187, 1535, 1682
PflFI	GACNNNGTC	1 cut(s)	2102
PflMI	CCANNNNNTGG	1 cut(s)	180
PinAI	ACCGGT	1 cut(s)	444
PkrI	GCNGC	5 cut(s)	137, 260, 385, 1392, 1654
Ple19I	CGATCG	1 cut(s)	408
PleI	GAGTC	2 cut(s)	505, 1582
PpsI	GAGTC	2 cut(s)	505, 1582
PpuMI	RGGWCCY	2 cut(s)	102, 1177
PsiI	TTATAA	2 cut(s)	771, 1754
Psp124BI	GAGCTC	1 cut(s)	1613
Psp5II	RGGWCCY	2 cut(s)	102, 1177
Psp6I	CCWGG	1 cut(s)	276
PspFI	CCCAGC	1 cut(s)	1472
PspGI	CCWGG	1 cut(s)	276
PspN4I	GGNNCC	2 cut(s)	368, 1179
PspPI	GGNCC	7 cut(s)	102, 280, 300, 417, 442, 1177, 1444
PspPPI	RGGWCCY	2 cut(s)	102, 1177
PspXI	VCTCGAGB	1 cut(s)	437
PstNI	CAGNNNCTG	2 cut(s)	1396, 2041
PsuI	RGATCY	2 cut(s)	1414, 1747
PsyI	GACNNNGTC	1 cut(s)	2102
PvuI	CGATCG	1 cut(s)	408
RsaI	GTAC	4 cut(s)	1306, 1343, 1478, 2003
RsaNI	GTAC	4 cut(s)	1305, 1342, 1477, 2002
RseI	CAYNNNNRTG	3 cut(s)	1103, 1119, 1160
SacI	GAGCTC	1 cut(s)	1613
SapI	GCTCTTC	1 cut(s)	1601
SatI	GCNGC	5 cut(s)	136, 259, 384, 1391, 1653
Sau3AI	GATC	6 cut(s)	405, 1294, 1414, 1437, 1747, 2084
Sau96I	GGNCC	7 cut(s)	102, 280, 300, 417, 442, 1177, 1444
ScaI	AGTACT	1 cut(s)	1343
SchI	GAGTC	2 cut(s)	506, 1582
ScrFI	CCNGG	2 cut(s)	22, 278
SduI	GDGCHC	1 cut(s)	1613
SfaNI	GCATC	6 cut(s)	100, 270, 1095, 1485, 1590, 1797
SfcI	CTRYAG	1 cut(s)	1450
Sfr274I	CTCGAG	1 cut(s)	437
SinI	GGWCC	4 cut(s)	102, 417, 442, 1177
SlaI	CTCGAG	1 cut(s)	437
SmiMI	CAYNNNNRTG	3 cut(s)	1103, 1119, 1160
SmlI	CTYRAG	3 cut(s)	350, 437, 581
SmoI	CTYRAG	3 cut(s)	350, 437, 581
SpeI	ACTAGT	2 cut(s)	596, 953
Sse9I	AATT	3 cut(s)	53, 287, 2013
SsiI	CCGC	7 cut(s)	70, 144, 256, 259, 384, 1780, 1912
SspI	AATATT	2 cut(s)	1732, 1896
SspMI	CTAG	7 cut(s)	387, 597, 954, 1025, 1487, 1592, 2069
SstI	GAGCTC	1 cut(s)	1613
StyD4I	CCNGG	2 cut(s)	20, 276
StyI	CCWWGG	4 cut(s)	130, 153, 179, 699
TaaI	ACNGT	3 cut(s)	40, 241, 1451
TaiI	ACGT	3 cut(s)	665, 1088, 1094
TaqI	TCGA	7 cut(s)	404, 408, 438, 572, 1524, 1829, 1984
TasI	AATT	3 cut(s)	53, 287, 2013
TatI	WGTACW	3 cut(s)	1304, 1341, 2001
TauI	GCSGC	2 cut(s)	261, 386
TfiI	GAWTC	5 cut(s)	373, 862, 1187, 1535, 1682
TscAI	CASTG	2 cut(s)	1454, 1774
TseI	GCWGC	3 cut(s)	135, 1390, 1652
TspDTI	ATGAA	7 cut(s)	331, 536, 636, 707, 707, 1392, 2022
TspGWI	ACGGA	1 cut(s)	1836
TspRI	CASTG	2 cut(s)	1454, 1774
Tth111I	GACNNNGTC	1 cut(s)	2102
Van91I	CCANNNNNTGG	1 cut(s)	180
Vha464I	CTTAAG	1 cut(s)	581
VpaK11BI	GGWCC	4 cut(s)	102, 417, 442, 1177
XapI	RAATTY	1 cut(s)	287
XbaI	TCTAGA	1 cut(s)	1591
XceI	RCATGY	2 cut(s)	1102, 1890
XhoI	CTCGAG	1 cut(s)	437
XmnI	GAANNNNTTC	1 cut(s)	1151
XspI	CTAG	7 cut(s)	387, 597, 954, 1025, 1487, 1592, 2069
ZrmI	AGTACT	1 cut(s)	1343
Zsp2I	ATGCAT	2 cut(s)	1110, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.