Rw1G016590

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005468
Physical Location & Seq
Forward (+)
36741089 .. 36746509
5421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016590.1

Sequence Viewer

Length: 2283 bp
ATGGCCGCCAAACCCATTCCCCGGCGACCAGCCTTGACAGTTCTCCGAGAGCAATTCCTCAGACCCAACCGCAACTCCAAACCTTCTCCTCCTCCTCTCAGGTCCTCCGATGCTGAAAACCCCGAAAAGCCCAAATCCCAAGGCAGCAAAACCGCCAAAGCCATGTCGTCGCTCATCAACTCCAACCCATGGTCATCTCGCCTCCAGTCTTCTCTCTCTTCTCTCTCTCCCTCCATCTCCACCACCACCGTCTCCCAAACCCTCCGCCGCATCAAAACCCCAACCCAAGCCCTCAAATTCTTCAACTGGGCCGAAGCAATGGGCTTCTCCCACAACCCCCAATCCTTCTTCATGTTGCTTGAGATTTTCGGCCGGAACCGGAATCTCAATGCCGCTAGAAATCTCTTGTTTTCGATTGAGAAGAGGTCCAATGGGAAGGTTAAGCTCGAGGACCGGTTTTTTAATAGCTTGATTAGGAGCTATGGGGAAGCAGGGTTGTTCCAGGAGTCTGTAAAGCTTTTTGGGACGATGAAGTCAATGGGGATATCGGCTTCTGTTGTTACTTTCAATAGTTTGCTTTCGATTTTGCTTAAGAGGGGTAGGACTAGTATGGCTAAGAATGTTTATGATGAAATGCTTGGGATGTATGGAGTTACGCCCGACACGCATACGTTTAATATTTTGATTAGGGGGTTCTGTATGAACTCCATGGTTGATGAGGGTTTTCATTATTTTAAGGAAATGTCAAGGTTGAAATGTGAGCCGGATGTGGTTACTTATAATACGATTGTTGATGGGTTGTGTAGAGCAGGGAAGGTTGAGATTGCGCGTAATGTGGTGAAGGGGATGAGGAAGAGAAGTGGGGAGTTGAATCCGAATGTTGTTACTTATACGACATTGATTAGAGGGTATTGTGTGAAAGAAGATGTTGATGAGGCTTTGTGTGTTTTGGAGGAGATGACTAGTCAAGGGTTGAAACCAAATGAAATTACCTATAATACTCTGCTTAAAGGGCTTTGTGAAGCAAAGAAGCTAGATAAGGTGAAAGAAGTCTTGGAGGGAACAATGAGAGGTGAAGGATTTACTCCTGATACGTGCACGTTCAACACGCTCATGCATTCGCATTGCAATGCAGGAAACGTAGAGGAAGCATTGAAGGTTTTCACAATGATGTCAGAGTTACAGGTCCCTCCGGATTCAGCGACATACAGCGTTTTGATACGCAGTTTGTGTCAGCAAAGAGATTATGGTAAAGCTGAGGAGTTGTTTGATGAGCTATCCAAGAAACAAATCTTGTTAAGTGATCGTGGCTGTACACCTGTTGTTGCCTCATACAAACCTATCTTTGAGTACTTATGTAGTAATGGGAGAACTAAGAAAGCAGATGAAGTGTTTAGGCAGCTACTGAAAAGAGGAACACAAGATCCTCCATCCTACAAGACTTTGATCATGGGCTACTGTAGGGAAGGCACATTTGAAGCTGGGTACAAACTTCTAGTCTTGATGCTTAGAAGGGATTTTGTTCCTGATGTCGAGATTTATGATTCCTTGATTGATGGTCTGTTGGAAAAGGGTAAGCCCCTTCTTGCACAACAGACTCTAGAGAAGATGCTGAAGAGCTCTCATCTCCCAAAAACATCTACTTTCCATTCTATACTGGCAGCTCTTTTAGAAATGCATTGTGCCCGTGAATCTGCAATCCTTGTTATTTTAATGTTGGAAAAAAACATTAGACAAAATATTAATCTCTCAACAGATCTTATAAGGCTCCTTTTCAGTGAGGGAATGCGAGATAAAGCATTTGAGATTGTAGGGATGCTGCATGATAACGGATACTCGATTAAAATAGAAGAAGTGGTCGAGTTCCTTTGCCAGATTGGAAAGCTATTAGAGGCATGTGAAATGTTGCAATTTAGTTTGCGGAAGCAAGAAAATGTCAGTATTGATACTTTTAACCAAGTTATATTAGGGCTATGTGATATCAATAAACTTCGAGAAGCGTTTGGCTTGTACTATCTACTGATTGAGAATGGCGACCATCAGCAGTTGCCGTGCTTAAATCATCTAAAATCTGCCCTAGAGGTTGCAGGTAGATCAGTTGAAGCTGACTTTGTCTCTAAAAGGATGCCAAAACATCAGCTGGTGGACAAATCTGGGAAGTCAAGACCTCAACTCATATCAAGACCTTCTCAGTTGCCCAAAGATTCTGGTTATGTGAAATTTGGACATAACAGAGAAGACAATGGAGTAGTGCGTCATGAATGGGCCTCCGCAGCTCAGTGA

Protein Analysis

760

Amino Acids

85.7

Weight (kDa)

8.99

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 154 - 193 9.5e-07 PPR repeat family
PPR_2 PF13041 185 - 233 2.5e-13 PPR repeat family
PPR_1 PF12854 217 - 249 4.1e-08 PPR repeat
PPR_2 PF13041 220 - 269 3.8e-16 PPR repeat family
PPR PF01535 224 - 253 8.9e-06 PPR repeat
PPR_long PF17177 235 - 355 1.5e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 253 - 284 2.2e-12 PPR repeat
PPR_2 PF13041 255 - 288 1.6e-07 PPR repeat family
TPR_24 PF23276 269 - 353 1.7e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 290 - 321 5.7e-11 PPR repeat
PPR_3 PF13812 290 - 337 7.8e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 292 - 340 1.7e-19 PPR repeat family
PPR PF01535 295 - 325 1.2e-07 PPR repeat
PPR_3 PF13812 316 - 373 8.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 324 - 352 1e-07 PPR repeat
PPR_3 PF13812 356 - 408 5.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 360 - 391 9.5e-11 PPR repeat
PPR_2 PF13041 363 - 412 1.2e-16 PPR repeat family
PPR PF01535 367 - 394 2.9e-06 PPR repeat
PPR_1 PF12854 396 - 425 4.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 780, 1763
AasI GACNNNNNNGTC 1 cut(s) 532
Acc36I ACCTGC 1 cut(s) 2078
AccB7I CCANNNNNTGG 1 cut(s) 189
AccII CGCG 1 cut(s) 829
AccIII TCCGGA 1 cut(s) 1192
AciI CCGC 8 cut(s) 6, 70, 153, 265, 268, 393, 1921, 2271
AclWI GGATC 1 cut(s) 1418
AcoI YGGCCR 2 cut(s) 3, 370
AcsI RAATTY 2 cut(s) 296, 2219
AcuI CTGAAG 1 cut(s) 1634
AfaI GTAC 4 cut(s) 1315, 1352, 1487, 2012
AfiI CCNNNNNNNGG 2 cut(s) 21, 189
AflII CTTAAG 1 cut(s) 590
AgeI ACCGGT 1 cut(s) 453
AgsI TTSAA 9 cut(s) 304, 568, 754, 871, 976, 1105, 1156, 1478, 2102
AhlI ACTAGT 2 cut(s) 605, 962
AjnI CCWGG 1 cut(s) 501
AjuI GAANNNNNNNTTGG 2 cut(s) 1037, 1069
AloI GAACNNNNNNTCC 2 cut(s) 1408, 1440
Alw21I GWGCWC 2 cut(s) 1100, 1622
Alw26I GTCTC 2 cut(s) 256, 2119
Alw44I GTGCAC 1 cut(s) 1096
AlwI GGATC 1 cut(s) 1418
AlwNI CAGNNNCTG 1 cut(s) 1405
Ama87I CYCGRG 1 cut(s) 446
Aor13HI TCCGGA 1 cut(s) 1192
AoxI GGCC 4 cut(s) 3, 309, 370, 2265
ApaLI GTGCAC 1 cut(s) 1096
ApeKI GCWGC 5 cut(s) 144, 1399, 1661, 1819, 2273
ApoI RAATTY 2 cut(s) 296, 2219
ArsI GACNNNNNNTTYG 2 cut(s) 1482, 1514
AseI ATTAAT 1 cut(s) 1743
AsiGI ACCGGT 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 1160
AspLEI GCGC 1 cut(s) 829
AspS9I GGNCC 6 cut(s) 102, 309, 426, 451, 1186, 2265
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 3 cut(s) 850, 1054, 1085
AvaI CYCGRG 1 cut(s) 446
AvaII GGWCC 4 cut(s) 102, 426, 451, 1186
BaeGI GKGCMC 2 cut(s) 1100, 1687
BanII GRGCYC 1 cut(s) 1622
BbsI GAAGAC 2 cut(s) 201, 2244
Bbv12I GWGCWC 2 cut(s) 1100, 1622
BbvCI CCTCAGC 1 cut(s) 1257
BbvI GCAGC 4 cut(s) 156, 1411, 1673, 1806
BccI CCATC 5 cut(s) 242, 788, 1438, 1550, 2046
BceAI ACGGC 1 cut(s) 2035
BciT130I CCWGG 1 cut(s) 503
BciVI GTATCC 1 cut(s) 1826
BclI TGATCA 1 cut(s) 1446
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 2 cut(s) 256, 2119
BcuI ACTAGT 2 cut(s) 605, 962
BfaI CTAG 7 cut(s) 396, 606, 963, 1034, 1496, 1601, 2078
BfmI CTRYAG 1 cut(s) 1459
BfrI CTTAAG 1 cut(s) 590
BfuAI ACCTGC 1 cut(s) 2078
BfuI GTATCC 1 cut(s) 1826
BglII AGATCT 1 cut(s) 1756
BisI GCNGC 8 cut(s) 6, 145, 268, 393, 1400, 1662, 1820, 2274
BlsI GCNGC 8 cut(s) 7, 146, 269, 394, 1401, 1663, 1821, 2275
BmcAI AGTACT 1 cut(s) 1352
Bme1390I CCNGG 2 cut(s) 22, 503
Bme18I GGWCC 4 cut(s) 102, 426, 451, 1186
BmeT110I CYCGRG 1 cut(s) 446
BmgT120I GGNCC 6 cut(s) 102, 309, 426, 451, 1186, 2265
BmiI GGNNCC 3 cut(s) 377, 1188, 1769
BmrFI CCNGG 2 cut(s) 22, 503
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 6 cut(s) 100, 279, 1494, 1599, 1806, 2115
BmuI ACTGGG 1 cut(s) 316
BpiI GAAGAC 2 cut(s) 201, 2244
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 1257
BpuEI CTTGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 1095
BsaJI CCNNGG 4 cut(s) 20, 139, 188, 708
BsaWI WCCGGW 3 cut(s) 378, 453, 1192
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 189
Bse118I RCCGGY 1 cut(s) 453
Bse1I ACTGG 3 cut(s) 205, 311, 1662
Bse3DI GCAATG 3 cut(s) 324, 1123, 1135
BseAI TCCGGA 1 cut(s) 1192
BseBI CCWGG 1 cut(s) 503
BseDI CCNNGG 4 cut(s) 20, 139, 188, 708
BseGI GGATG 6 cut(s) 648, 772, 852, 1430, 1821, 2130
BseLI CCNNNNNNNGG 2 cut(s) 21, 189
BseMI GCAATG 3 cut(s) 324, 1123, 1135
BseMII CTCAG 4 cut(s) 73, 112, 1248, 2204
BseNI ACTGG 3 cut(s) 205, 311, 1662
BseRI GAGGAG 5 cut(s) 78, 81, 84, 968, 1274
BseSI GKGCMC 2 cut(s) 1100, 1687
BseX3I CGGCCG 1 cut(s) 370
BseXI GCAGC 4 cut(s) 156, 1411, 1673, 1806
BseYI CCCAGC 1 cut(s) 1481
Bsh1236I CGCG 1 cut(s) 829
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 4 cut(s) 5, 311, 372, 2267
BshTI ACCGGT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 373
BsiHKAI GWGCWC 2 cut(s) 1100, 1622
BsiHKCI CYCGRG 1 cut(s) 446
BsiSI CCGG 6 cut(s) 22, 373, 379, 454, 764, 1193
BslFI GGGAC 2 cut(s) 538, 1172
BslI CCNNNNNNNGG 2 cut(s) 21, 189
BsmAI GTCTC 2 cut(s) 256, 2119
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 2 cut(s) 538, 1172
BsmI GAATGC 2 cut(s) 1117, 1791
BsnI GGCC 4 cut(s) 5, 311, 372, 2267
BsoBI CYCGRG 1 cut(s) 446
Bsp1286I GDGCHC 3 cut(s) 1100, 1622, 1687
Bsp13I TCCGGA 1 cut(s) 1192
Bsp1407I TGTACA 1 cut(s) 1313
Bsp143I GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
Bsp19I CCATGG 2 cut(s) 188, 708
BspACI CCGC 8 cut(s) 6, 70, 153, 265, 268, 393, 1921, 2271
BspANI GGCC 4 cut(s) 5, 311, 372, 2267
BspCNI CTCAG 4 cut(s) 72, 111, 1249, 2203
BspEI TCCGGA 1 cut(s) 1192
BspFNI CGCG 1 cut(s) 829
BspHI TCATGA 1 cut(s) 2257
BspLI GGNNCC 3 cut(s) 377, 1188, 1769
BspMI ACCTGC 1 cut(s) 2078
BspPI GGATC 1 cut(s) 1418
BspQI GCTCTTC 1 cut(s) 1610
BspTI CTTAAG 1 cut(s) 590
BsrDI GCAATG 3 cut(s) 324, 1123, 1135
BsrFI RCCGGY 1 cut(s) 453
BsrGI TGTACA 1 cut(s) 1313
BsrI ACTGG 3 cut(s) 205, 311, 1662
BssAI RCCGGY 1 cut(s) 453
BssECI CCNNGG 4 cut(s) 20, 139, 188, 708
BssMI GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
BssT1I CCWWGG 3 cut(s) 139, 188, 708
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 3 cut(s) 40, 250, 1460
Bst6I CTCTTC 4 cut(s) 223, 416, 848, 1610
BstAFI CTTAAG 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 1313
BstBAI YACGTR 1 cut(s) 1095
BstDEI CTNAG 8 cut(s) 59, 98, 615, 1257, 1374, 1508, 2190, 2277
BstDSI CCRYGG 2 cut(s) 188, 708
BstF5I GGATG 6 cut(s) 648, 772, 852, 1430, 1821, 2130
BstFNI CGCG 1 cut(s) 829
BstHHI GCGC 1 cut(s) 829
BstKTI GATC 5 cut(s) 1306, 1426, 1449, 1759, 2096
BstMAI GTCTC 2 cut(s) 256, 2119
BstMBI GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 3 cut(s) 664, 1012, 2273
BstNI CCWGG 1 cut(s) 503
BstNSI RCATGY 1 cut(s) 1899
BstSCI CCNGG 2 cut(s) 20, 501
BstSFI CTRYAG 1 cut(s) 1459
BstSLI GKGCMC 2 cut(s) 1100, 1687
BstUI CGCG 1 cut(s) 829
BstV1I GCAGC 4 cut(s) 156, 1411, 1673, 1806
BstV2I GAAGAC 2 cut(s) 201, 2244
BstX2I RGATCY 2 cut(s) 1423, 1756
BstYI RGATCY 2 cut(s) 1423, 1756
BstZI CGGCCG 1 cut(s) 370
BsuI GTATCC 1 cut(s) 1826
BsuRI GGCC 4 cut(s) 5, 311, 372, 2267
BtgI CCRYGG 2 cut(s) 188, 708
BtsCI GGATG 6 cut(s) 648, 772, 852, 1430, 1821, 2130
BtsIMutI CAGTG 1 cut(s) 1783
BveI ACCTGC 1 cut(s) 2078
CaiI CAGNNNCTG 1 cut(s) 1405
CciI TCATGA 1 cut(s) 2257
CfoI GCGC 1 cut(s) 829
Cfr10I RCCGGY 1 cut(s) 453
Cfr13I GGNCC 6 cut(s) 102, 309, 426, 451, 1186, 2265
CseI GACGC 1 cut(s) 2243
Csp6I GTAC 4 cut(s) 1314, 1351, 1486, 2011
CspAI ACCGGT 1 cut(s) 453
CviAII CATG 9 cut(s) 163, 189, 352, 709, 1114, 1450, 1823, 1896, 2258
CviQI GTAC 4 cut(s) 1314, 1351, 1486, 2011
DdeI CTNAG 8 cut(s) 59, 98, 615, 1257, 1374, 1508, 2190, 2277
DpnI GATC 5 cut(s) 1305, 1425, 1448, 1758, 2095
DpnII GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
DrdI GACNNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 532
EaeI YGGCCR 2 cut(s) 3, 370
EagI CGGCCG 1 cut(s) 370
Eam1104I CTCTTC 4 cut(s) 223, 416, 848, 1610
EarI CTCTTC 4 cut(s) 223, 416, 848, 1610
EciI GGCGGA 1 cut(s) 254
Ecl136II GAGCTC 1 cut(s) 1620
EclXI CGGCCG 1 cut(s) 370
Eco130I CCWWGG 3 cut(s) 139, 188, 708
Eco24I GRGCYC 1 cut(s) 1622
Eco32I GATATC 2 cut(s) 546, 1981
Eco47I GGWCC 4 cut(s) 102, 426, 451, 1186
Eco52I CGGCCG 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 1620
Eco57I CTGAAG 1 cut(s) 1634
Eco88I CYCGRG 1 cut(s) 446
EcoICRI GAGCTC 1 cut(s) 1620
EcoO109I RGGNCCY 2 cut(s) 102, 1186
EcoRII CCWGG 1 cut(s) 501
EcoRV GATATC 2 cut(s) 546, 1981
EcoT14I CCWWGG 3 cut(s) 139, 188, 708
EcoT22I ATGCAT 2 cut(s) 1119, 1680
EcoT38I GRGCYC 1 cut(s) 1622
ErhI CCWWGG 3 cut(s) 139, 188, 708
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 9 cut(s) 166, 192, 355, 712, 1117, 1453, 1826, 1899, 2261
FaqI GGGAC 2 cut(s) 538, 1172
FatI CATG 9 cut(s) 162, 188, 351, 708, 1113, 1449, 1822, 1895, 2257
FbaI TGATCA 1 cut(s) 1446
Fnu4HI GCNGC 8 cut(s) 6, 145, 268, 393, 1400, 1662, 1820, 2274
FokI GGATG 6 cut(s) 655, 779, 859, 1417, 1828, 2137
FriOI GRGCYC 1 cut(s) 1622
Fsp4HI GCNGC 8 cut(s) 6, 145, 268, 393, 1400, 1662, 1820, 2274
FspBI CTAG 7 cut(s) 396, 606, 963, 1034, 1496, 1601, 2078
GlaI GCGC 1 cut(s) 828
GluI GCNGC 8 cut(s) 6, 145, 268, 393, 1400, 1662, 1820, 2274
GsaI CCCAGC 1 cut(s) 1485
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 4 cut(s) 5, 311, 372, 2267
HapII CCGG 6 cut(s) 22, 373, 379, 454, 764, 1193
HgaI GACGC 1 cut(s) 2243
HhaI GCGC 1 cut(s) 829
Hin1II CATG 9 cut(s) 166, 192, 355, 712, 1117, 1453, 1826, 1899, 2261
Hin6I GCGC 1 cut(s) 827
HinP1I GCGC 1 cut(s) 827
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 8 cut(s) 382, 506, 871, 1196, 1544, 1597, 1691, 2204
HpaII CCGG 6 cut(s) 22, 373, 379, 454, 764, 1193
HphI GGTGA 3 cut(s) 850, 1054, 1085
Hpy166II GTNNAC 3 cut(s) 1098, 1316, 2146
Hpy188I TCNGA 5 cut(s) 47, 62, 109, 876, 1177
Hpy8I GTNNAC 3 cut(s) 1098, 1316, 2146
Hpy99I CGWCG 1 cut(s) 172
HpyCH4III ACNGT 3 cut(s) 40, 250, 1460
HpyCH4IV ACGT 4 cut(s) 671, 1094, 1100, 1140
HpyF10VI GCNNNNNNNGC 3 cut(s) 664, 1012, 2273
HpyF3I CTNAG 8 cut(s) 59, 98, 615, 1257, 1374, 1508, 2190, 2277
HpySE526I ACGT 4 cut(s) 671, 1094, 1100, 1140
Hsp92II CATG 9 cut(s) 166, 192, 355, 712, 1117, 1453, 1826, 1899, 2261
HspAI GCGC 1 cut(s) 827
Kpn2I TCCGGA 1 cut(s) 1192
Ksp22I TGATCA 1 cut(s) 1446
Kzo9I GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
LguI GCTCTTC 1 cut(s) 1610
LmnI GCTCC 2 cut(s) 477, 1773
Lsp1109I GCAGC 4 cut(s) 156, 1411, 1673, 1806
LweI GCATC 6 cut(s) 100, 279, 1494, 1599, 1806, 2115
MaeI CTAG 7 cut(s) 396, 606, 963, 1034, 1496, 1601, 2078
MaeII ACGT 4 cut(s) 671, 1094, 1100, 1140
MaeIII GTNAC 5 cut(s) 559, 652, 772, 883, 1179
MalI GATC 5 cut(s) 1305, 1425, 1448, 1758, 2095
MboI GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
MflI RGATCY 2 cut(s) 1423, 1756
MhlI GDGCHC 3 cut(s) 1100, 1622, 1687
MluCI AATT 5 cut(s) 53, 296, 987, 1910, 2219
MlyI GAGTC 2 cut(s) 515, 1591
MmeI TCCRAC 3 cut(s) 207, 1545, 1698
Mph1103I ATGCAT 2 cut(s) 1119, 1680
MroI TCCGGA 1 cut(s) 1192
MroXI GAANNNNTTC 1 cut(s) 1160
MslI CAYNNNNRTG 3 cut(s) 1112, 1128, 1169
MspA1I CMGCKG 1 cut(s) 2140
MspCI CTTAAG 1 cut(s) 590
MspI CCGG 6 cut(s) 22, 373, 379, 454, 764, 1193
MspR9I CCNGG 2 cut(s) 22, 503
Mva1269I GAATGC 2 cut(s) 1117, 1791
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 829
MwoI GCNNNNNNNGC 3 cut(s) 664, 1012, 2273
NciI CCSGG 1 cut(s) 22
NcoI CCATGG 2 cut(s) 188, 708
NdeII GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
NlaIII CATG 9 cut(s) 166, 192, 355, 712, 1117, 1453, 1826, 1899, 2261
NlaIV GGNNCC 3 cut(s) 377, 1188, 1769
NsiI ATGCAT 2 cut(s) 1119, 1680
NspI RCATGY 1 cut(s) 1899
PaeR7I CTCGAG 1 cut(s) 446
PagI TCATGA 1 cut(s) 2257
PciSI GCTCTTC 1 cut(s) 1610
PctI GAATGC 2 cut(s) 1117, 1791
PdmI GAANNNNTTC 1 cut(s) 1160
PfeI GAWTC 6 cut(s) 382, 871, 1196, 1544, 1691, 2204
PflFI GACNNNGTC 1 cut(s) 2111
PflMI CCANNNNNTGG 1 cut(s) 189
PfoI TCCNGGA 1 cut(s) 501
PinAI ACCGGT 1 cut(s) 453
PkrI GCNGC 8 cut(s) 7, 146, 269, 394, 1401, 1663, 1821, 2275
PleI GAGTC 2 cut(s) 514, 1591
PpsI GAGTC 2 cut(s) 514, 1591
Ppu21I YACGTR 1 cut(s) 1095
PpuMI RGGWCCY 2 cut(s) 102, 1186
PshBI ATTAAT 1 cut(s) 1743
PsiI TTATAA 2 cut(s) 780, 1763
Psp124BI GAGCTC 1 cut(s) 1622
Psp5II RGGWCCY 2 cut(s) 102, 1186
Psp6I CCWGG 1 cut(s) 501
PspFI CCCAGC 1 cut(s) 1481
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 3 cut(s) 377, 1188, 1769
PspPI GGNCC 6 cut(s) 102, 309, 426, 451, 1186, 2265
PspPPI RGGWCCY 2 cut(s) 102, 1186
PspXI VCTCGAGB 1 cut(s) 446
PstNI CAGNNNCTG 1 cut(s) 1405
PsuI RGATCY 2 cut(s) 1423, 1756
PsyI GACNNNGTC 1 cut(s) 2111
PvuII CAGCTG 1 cut(s) 2140
RsaI GTAC 4 cut(s) 1315, 1352, 1487, 2012
RsaNI GTAC 4 cut(s) 1314, 1351, 1486, 2011
RseI CAYNNNNRTG 3 cut(s) 1112, 1128, 1169
SacI GAGCTC 1 cut(s) 1622
SapI GCTCTTC 1 cut(s) 1610
SatI GCNGC 8 cut(s) 6, 145, 268, 393, 1400, 1662, 1820, 2274
Sau3AI GATC 5 cut(s) 1303, 1423, 1446, 1756, 2093
Sau96I GGNCC 6 cut(s) 102, 309, 426, 451, 1186, 2265
ScaI AGTACT 1 cut(s) 1352
SchI GAGTC 2 cut(s) 515, 1591
ScrFI CCNGG 2 cut(s) 22, 503
SduI GDGCHC 3 cut(s) 1100, 1622, 1687
SfaNI GCATC 6 cut(s) 100, 279, 1494, 1599, 1806, 2115
SfcI CTRYAG 1 cut(s) 1459
Sfr274I CTCGAG 1 cut(s) 446
SinI GGWCC 4 cut(s) 102, 426, 451, 1186
SlaI CTCGAG 1 cut(s) 446
SmiMI CAYNNNNRTG 3 cut(s) 1112, 1128, 1169
SmlI CTYRAG 3 cut(s) 359, 446, 590
SmoI CTYRAG 3 cut(s) 359, 446, 590
SpeI ACTAGT 2 cut(s) 605, 962
Sse9I AATT 5 cut(s) 53, 296, 987, 1910, 2219
SsiI CCGC 8 cut(s) 6, 70, 153, 265, 268, 393, 1921, 2271
SspI AATATT 2 cut(s) 679, 1741
SspMI CTAG 7 cut(s) 396, 606, 963, 1034, 1496, 1601, 2078
SstI GAGCTC 1 cut(s) 1622
StyD4I CCNGG 2 cut(s) 20, 501
StyI CCWWGG 3 cut(s) 139, 188, 708
TaaI ACNGT 3 cut(s) 40, 250, 1460
TaiI ACGT 4 cut(s) 674, 1097, 1103, 1143
TaqI TCGA 7 cut(s) 413, 447, 581, 1533, 1838, 1860, 1993
TasI AATT 5 cut(s) 53, 296, 987, 1910, 2219
TatI WGTACW 3 cut(s) 1313, 1350, 2010
TauI GCSGC 3 cut(s) 8, 270, 395
TfiI GAWTC 6 cut(s) 382, 871, 1196, 1544, 1691, 2204
TscAI CASTG 1 cut(s) 1783
TseI GCWGC 5 cut(s) 144, 1399, 1661, 1819, 2273
TspDTI ATGAA 8 cut(s) 340, 545, 645, 716, 716, 999, 1401, 2274
TspGWI ACGGA 1 cut(s) 1845
TspRI CASTG 1 cut(s) 1783
Tth111I GACNNNGTC 1 cut(s) 2111
Van91I CCANNNNNTGG 1 cut(s) 189
Vha464I CTTAAG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 1096
VpaK11BI GGWCC 4 cut(s) 102, 426, 451, 1186
VspI ATTAAT 1 cut(s) 1743
XapI RAATTY 2 cut(s) 296, 2219
XbaI TCTAGA 1 cut(s) 1600
XceI RCATGY 1 cut(s) 1899
XhoI CTCGAG 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 1160
XspI CTAG 7 cut(s) 396, 606, 963, 1034, 1496, 1601, 2078
ZrmI AGTACT 1 cut(s) 1352
Zsp2I ATGCAT 2 cut(s) 1119, 1680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.