RchiOBHm_Chr1g0346171

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005468
Physical Location & Seq
Forward (+)
38310922 .. 38316862
5941 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57245

Sequence Viewer

Length: 2172 bp
ATGGCGGCCATACCCATTTCCCGGCGACCAGCCTTGACAGTTCTCCGAGAGCAATTCCTCAGACCCAACCGCAACTCCAAACCTCCTCCTCCTCCTCCTCTCAGGTCCTCCGATGCCGAAAAGCCCAAATCCCAAGGCAGCAAAACCGCCAAAGCCATGGCGTCGCTCATCAACTCCAACCCATGGTCATCTCGCCTCCAGTCTTCTCTCTCTTCTCTCTCTCCCTCCATCTCCACCACCACCGTCTCCCAAACCCTCCGCCGCATCAAAACCCCAACCCAAGCCCTCAAATTCTTCAACTGGGCCGAATCAATGGGCTTCTCCCACAACGCCCAATCCTTCTTCATGTTGCTTGAGATTTTCGGCCGGAACCGGAATCTCAATGCCGCTAGAAATCTCTTGTTTTCAATTGAGAAGAGGTCCAATGGGAAGGTTAAGCTCGAGGACCGGTTTTTTAATAGCTTGATTAGGAGCTATGGGGAAGCAGGGTTGTTTCAGGAGTCTGTAAAGCTTTTTGGGACGATGAAGTCGATGGGGATATCGGCTTCTGTTGTTAGTTTCAATAGTTTGCTTTCGATTTTGCTTAAGAGGGGAAGGACTAGTATGGCTAAGAATGTTTATGATGAAATGCTTGGGATGTATGGAGTTACGCCCGATACGCATACGTTTAATATTTTGATTAGGGGGTTCTGTATGAACTCCATGGTTGATGAGGGTTTTCATTATTTTAAGGAAATGTCAAGGTTGAAATGTGAGCCGGATGTGGTTACTTATAATACGATTGTTGATGGGTTGTGTAGAGCAGGGAAGGTTGAGATTGCGCGAAATGTGGTGAAGGGGATGAGGAAGAGAAGTGGGGAGTTGAATCCGAATGTTGTTACTTATACGACATTGATTAGAGGGTATTGTGTGAAAGAAGATGTTGATGAGGCTTTGTGTGTTTTGGAGGAGATGGCTAGTCAAGGGTTGAAACCAAATGATATTACCTATAATACTCTGCTTAAAGGGCTTTGTGAAGCAAAGAAGCTAGATAAGATCAAAGAAGTCTTGGAGGGAACAATGAGAGGTGAAGGATTTACTCCTAATACGTGTACATTCAACACACTGATGCATTCGCATTGCAATGCAGGAAACTTAGAGGAAGCATTGAAGGTTTTCACAATGATGTCAGAGTTACAGGTCCCTTCAGACTCAGCTACATATAGTGTTTTGATACGCAGTTTGTGTCAGCGAAGAGATTATGGTAAAGCTGAGGAGTTGTTTGATGAGCTATCCAAGAAACAAATCTTGTTAAGTGATCGTGGCTGTACACCTGTTGTTGCCTCATACAAACCTATCTTTGAGTACTTATGTAGTAATGGGAGAACTAAGAAAGCAGATGAAGTGTTTAGGCAGCTACTGAAAAGAGGAACACAAGATCCTCCATCCTACAAGACTTTGATCATGGGCCACTGTAGGGAAGGCACATTCGAAGCTGGGTACAAACTTCTAGTCTTGATGCTTAGAAGGGATTTTGTTCCTGATGTTGAGATTTATGATTCCTTGATTGATGGTCTGTTGGAAAAGGGTAAGCCCCTTCTTGCACAACAGACTCTAGAGAAGATGCTGAAGAGCTCTCATCTCCCAAAAACATCTACTTTCCATTCTATACTGGCAGCTCTTTTAGAAATGCATTGTGCCCGTGAATCTGCCAGCTTTGTTATTTTAATGTTGGAGAAAAACATTAGACAAAATATTAACCTCTCCACAGATCTTGTAAGGCTTCTTTTCAGTGAGGGATTGCGAGATAAAGCATTTGAGATTGTAGGGATGCTGCATGATAATGGATACTTGATTAAAATAGAAGAAGTGGTCAGTTTCCTTTACCAGAGCAGAAAGTTAATAGAGGCATGTGAAATATTGCGATTTAGTCTGCGCAAGCAAGCAAATGTCAGTATTGATATATTTGACCAAGTTCTATTAGGGCTATGTGATATCAATAAGCTTCGAGAAGCATTTGGCTTGTACTATGAACTGATTGAGAATGGTGACCACCGGCAGTTGCCGTGCTTACATCATTTAAAGTCTTCCCTAGAGGTTGCAGGTAGATCAGTTGAAGCTGACTTTGTCTCTAAAAGAATGCCAAAACATCAGCTGGTGGACAAATTTGGGAAGTCGAGACCTCAACTATGA

Protein Analysis

723

Amino Acids

81.65

Weight (kDa)

9.18

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 183 - 231 7.4e-13 PPR repeat family
PPR_long PF17177 203 - 353 1.1e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 215 - 247 3.8e-08 PPR repeat
PPR_2 PF13041 218 - 267 3.6e-16 PPR repeat family
PPR PF01535 222 - 251 8.4e-06 PPR repeat
PPR_3 PF13812 243 - 300 2.2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 251 - 282 2.1e-12 PPR repeat
PPR_2 PF13041 253 - 286 1.5e-07 PPR repeat family
TPR_24 PF23276 267 - 351 1.4e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 288 - 318 6.9e-11 PPR repeat
PPR_3 PF13812 288 - 335 1.4e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 290 - 338 3.7e-19 PPR repeat family
PPR PF01535 293 - 322 2e-07 PPR repeat
PPR_3 PF13812 314 - 371 5.1e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 322 - 345 3.4e-07 PPR repeat
PPR_1 PF12854 358 - 389 1.8e-10 PPR repeat
PPR_2 PF13041 361 - 410 5.2e-15 PPR repeat family
PPR PF01535 365 - 392 3.1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
AasI GACNNNNNNGTC 1 cut(s) 526
Acc16I TGCGCA 1 cut(s) 1916
Acc36I ACCTGC 1 cut(s) 2072
AccB7I CCANNNNNTGG 1 cut(s) 183
AccII CGCG 1 cut(s) 823
AciI CCGC 6 cut(s) 5, 70, 147, 259, 262, 387
AclWI GGATC 1 cut(s) 1412
AcoI YGGCCR 2 cut(s) 6, 364
AcsI RAATTY 2 cut(s) 290, 2144
AcuI CTGAAG 2 cut(s) 1170, 1628
AcyI GRCGYC 1 cut(s) 161
AfaI GTAC 5 cut(s) 1093, 1309, 1346, 1481, 2006
AfiI CCNNNNNNNGG 3 cut(s) 21, 183, 1456
AflII CTTAAG 1 cut(s) 584
AflIII ACRYGT 1 cut(s) 1088
AgeI ACCGGT 1 cut(s) 447
AgsI TTSAA 9 cut(s) 298, 408, 562, 748, 865, 970, 1099, 1150, 2096
AhlI ACTAGT 1 cut(s) 599
AloI GAACNNNNNNTCC 2 cut(s) 1402, 1434
Alw21I GWGCWC 1 cut(s) 1616
Alw26I GTCTC 3 cut(s) 250, 2113, 2152
AlwI GGATC 1 cut(s) 1412
AlwNI CAGNNNCTG 1 cut(s) 1399
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 4 cut(s) 6, 303, 364, 1447
ApeKI GCWGC 4 cut(s) 138, 1393, 1655, 1813
ApoI RAATTY 2 cut(s) 290, 2144
ArsI GACNNNNNNTTYG 2 cut(s) 1476, 1508
AsiGI ACCGGT 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 1154
AspLEI GCGC 2 cut(s) 823, 1917
AspS9I GGNCC 6 cut(s) 105, 303, 420, 445, 1180, 1447
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 3 cut(s) 844, 1079, 2039
AsuII TTCGAA 1 cut(s) 1470
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 4 cut(s) 105, 420, 445, 1180
BaeGI GKGCMC 1 cut(s) 1681
BanII GRGCYC 1 cut(s) 1616
BbsI GAAGAC 2 cut(s) 195, 2058
Bbv12I GWGCWC 1 cut(s) 1616
BbvCI CCTCAGC 1 cut(s) 1251
BbvI GCAGC 4 cut(s) 150, 1405, 1667, 1800
BccI CCATC 6 cut(s) 236, 526, 782, 946, 1432, 1544
BceAI ACGGC 1 cut(s) 2029
BciVI GTATCC 1 cut(s) 1820
BclI TGATCA 1 cut(s) 1440
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 3 cut(s) 250, 2113, 2152
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 7 cut(s) 390, 600, 957, 1028, 1490, 1595, 2072
BfmI CTRYAG 1 cut(s) 1453
BfrI CTTAAG 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 2072
BfuI GTATCC 1 cut(s) 1820
BglII AGATCT 1 cut(s) 1750
BisI GCNGC 7 cut(s) 6, 139, 262, 387, 1394, 1656, 1814
BlsI GCNGC 7 cut(s) 7, 140, 263, 388, 1395, 1657, 1815
BmcAI AGTACT 1 cut(s) 1346
Bme1390I CCNGG 1 cut(s) 22
Bme18I GGWCC 4 cut(s) 105, 420, 445, 1180
BmeT110I CYCGRG 1 cut(s) 440
BmgT120I GGNCC 6 cut(s) 105, 303, 420, 445, 1180, 1447
BmiI GGNNCC 2 cut(s) 371, 1182
BmrFI CCNGG 1 cut(s) 22
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 6 cut(s) 103, 273, 1098, 1488, 1593, 1800
BmuI ACTGGG 1 cut(s) 310
BpiI GAAGAC 2 cut(s) 195, 2058
BpmI CTGGAG 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 1251
Bpu14I TTCGAA 1 cut(s) 1470
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 1089
BsaHI GRCGYC 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 2152
BsaJI CCNNGG 4 cut(s) 133, 156, 182, 702
BsaWI WCCGGW 2 cut(s) 372, 447
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 183, 1456
Bse118I RCCGGY 2 cut(s) 447, 2034
Bse1I ACTGG 3 cut(s) 199, 305, 1656
Bse3DI GCAATG 2 cut(s) 1117, 1129
BseDI CCNNGG 4 cut(s) 133, 156, 182, 702
BseGI GGATG 5 cut(s) 642, 766, 846, 1424, 1815
BseLI CCNNNNNNNGG 3 cut(s) 21, 183, 1456
BseMI GCAATG 2 cut(s) 1117, 1129
BseMII CTCAG 4 cut(s) 73, 115, 1206, 1242
BseNI ACTGG 3 cut(s) 199, 305, 1656
BseRI GAGGAG 7 cut(s) 75, 78, 81, 84, 87, 962, 1268
BseSI GKGCMC 1 cut(s) 1681
BseX3I CGGCCG 1 cut(s) 364
BseXI GCAGC 4 cut(s) 150, 1405, 1667, 1800
BseYI CCCAGC 1 cut(s) 1475
Bsh1236I CGCG 1 cut(s) 823
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 4 cut(s) 8, 305, 366, 1449
BshTI ACCGGT 1 cut(s) 447
BsiEI CGRYCG 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 1616
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 6 cut(s) 22, 367, 373, 448, 758, 2035
BslFI GGGAC 2 cut(s) 532, 1166
BslI CCNNNNNNNGG 3 cut(s) 21, 183, 1456
BsmAI GTCTC 3 cut(s) 250, 2113, 2152
BsmBI CGTCTC 1 cut(s) 250
BsmFI GGGAC 2 cut(s) 532, 1166
BsmI GAATGC 2 cut(s) 1111, 2124
BsnI GGCC 4 cut(s) 8, 305, 366, 1449
Bso31I GGTCTC 1 cut(s) 2152
BsoBI CYCGRG 1 cut(s) 440
Bsp119I TTCGAA 1 cut(s) 1470
Bsp1286I GDGCHC 2 cut(s) 1616, 1681
Bsp1407I TGTACA 2 cut(s) 1091, 1307
Bsp143I GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
Bsp19I CCATGG 3 cut(s) 156, 182, 702
BspACI CCGC 6 cut(s) 5, 70, 147, 259, 262, 387
BspANI GGCC 4 cut(s) 8, 305, 366, 1449
BspCNI CTCAG 4 cut(s) 72, 114, 1205, 1243
BspFNI CGCG 1 cut(s) 823
BspLI GGNNCC 2 cut(s) 371, 1182
BspMI ACCTGC 1 cut(s) 2072
BspPI GGATC 1 cut(s) 1412
BspQI GCTCTTC 1 cut(s) 1604
BspT104I TTCGAA 1 cut(s) 1470
BspTI CTTAAG 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 2152
BsrDI GCAATG 2 cut(s) 1117, 1129
BsrFI RCCGGY 2 cut(s) 447, 2034
BsrGI TGTACA 2 cut(s) 1091, 1307
BsrI ACTGG 3 cut(s) 199, 305, 1656
BssAI RCCGGY 2 cut(s) 447, 2034
BssECI CCNNGG 4 cut(s) 133, 156, 182, 702
BssMI GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
BssNI GRCGYC 1 cut(s) 161
BssT1I CCWWGG 4 cut(s) 133, 156, 182, 702
Bst4CI ACNGT 3 cut(s) 40, 244, 1454
Bst6I CTCTTC 5 cut(s) 217, 410, 842, 1228, 1604
BstACI GRCGYC 1 cut(s) 161
BstAFI CTTAAG 1 cut(s) 584
BstAUI TGTACA 2 cut(s) 1091, 1307
BstBAI YACGTR 1 cut(s) 1089
BstBI TTCGAA 1 cut(s) 1470
BstC8I GCNNGC 3 cut(s) 1693, 1919, 1923
BstDEI CTNAG 8 cut(s) 59, 101, 609, 1135, 1192, 1251, 1368, 1502
BstDSI CCRYGG 3 cut(s) 156, 182, 702
BstEII GGTNACC 1 cut(s) 2027
BstF5I GGATG 5 cut(s) 642, 766, 846, 1424, 1815
BstFNI CGCG 1 cut(s) 823
BstHHI GCGC 2 cut(s) 823, 1917
BstKTI GATC 6 cut(s) 1038, 1300, 1420, 1443, 1753, 2090
BstMAI GTCTC 3 cut(s) 250, 2113, 2152
BstMBI GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 2 cut(s) 658, 1006
BstNSI RCATGY 1 cut(s) 1893
BstPI GGTNACC 1 cut(s) 2027
BstSCI CCNGG 1 cut(s) 20
BstSFI CTRYAG 1 cut(s) 1453
BstSLI GKGCMC 1 cut(s) 1681
BstUI CGCG 1 cut(s) 823
BstV1I GCAGC 4 cut(s) 150, 1405, 1667, 1800
BstV2I GAAGAC 2 cut(s) 195, 2058
BstX2I RGATCY 2 cut(s) 1417, 1750
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 2 cut(s) 1417, 1750
BstZI CGGCCG 1 cut(s) 364
BsuI GTATCC 1 cut(s) 1820
BsuRI GGCC 4 cut(s) 8, 305, 366, 1449
BtgI CCRYGG 3 cut(s) 156, 182, 702
BtsCI GGATG 5 cut(s) 642, 766, 846, 1424, 1815
BtsIMutI CAGTG 3 cut(s) 1103, 1450, 1777
BveI ACCTGC 1 cut(s) 2072
Cac8I GCNNGC 3 cut(s) 1693, 1919, 1923
CaiI CAGNNNCTG 1 cut(s) 1399
CfoI GCGC 2 cut(s) 823, 1917
Cfr10I RCCGGY 2 cut(s) 447, 2034
Cfr13I GGNCC 6 cut(s) 105, 303, 420, 445, 1180, 1447
CseI GACGC 1 cut(s) 150
Csp6I GTAC 5 cut(s) 1092, 1308, 1345, 1480, 2005
CspAI ACCGGT 1 cut(s) 447
CspCI CAANNNNNGTGG 2 cut(s) 1735, 1770
CviAII CATG 7 cut(s) 157, 183, 346, 703, 1444, 1817, 1890
CviQI GTAC 5 cut(s) 1092, 1308, 1345, 1480, 2005
DdeI CTNAG 8 cut(s) 59, 101, 609, 1135, 1192, 1251, 1368, 1502
DpnI GATC 6 cut(s) 1037, 1299, 1419, 1442, 1752, 2089
DpnII GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
DraI TTTAAA 1 cut(s) 2061
DrdI GACNNNNNNGTC 1 cut(s) 526
DseDI GACNNNNNNGTC 1 cut(s) 526
EaeI YGGCCR 2 cut(s) 6, 364
EagI CGGCCG 1 cut(s) 364
Eam1104I CTCTTC 5 cut(s) 217, 410, 842, 1228, 1604
EarI CTCTTC 5 cut(s) 217, 410, 842, 1228, 1604
EciI GGCGGA 1 cut(s) 248
Ecl136II GAGCTC 1 cut(s) 1614
EclXI CGGCCG 1 cut(s) 364
Eco130I CCWWGG 4 cut(s) 133, 156, 182, 702
Eco24I GRGCYC 1 cut(s) 1616
Eco31I GGTCTC 1 cut(s) 2152
Eco32I GATATC 2 cut(s) 540, 1975
Eco47I GGWCC 4 cut(s) 105, 420, 445, 1180
Eco52I CGGCCG 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 1614
Eco57I CTGAAG 2 cut(s) 1170, 1628
Eco88I CYCGRG 1 cut(s) 440
Eco91I GGTNACC 1 cut(s) 2027
EcoICRI GAGCTC 1 cut(s) 1614
EcoO109I RGGNCCY 2 cut(s) 105, 1180
EcoO65I GGTNACC 1 cut(s) 2027
EcoRV GATATC 2 cut(s) 540, 1975
EcoT14I CCWWGG 4 cut(s) 133, 156, 182, 702
EcoT22I ATGCAT 2 cut(s) 1113, 1674
EcoT38I GRGCYC 1 cut(s) 1616
ErhI CCWWGG 4 cut(s) 133, 156, 182, 702
Esp3I CGTCTC 1 cut(s) 250
FaeI CATG 7 cut(s) 160, 186, 349, 706, 1447, 1820, 1893
FaqI GGGAC 2 cut(s) 532, 1166
FatI CATG 7 cut(s) 156, 182, 345, 702, 1443, 1816, 1889
FbaI TGATCA 1 cut(s) 1440
Fnu4HI GCNGC 7 cut(s) 6, 139, 262, 387, 1394, 1656, 1814
FokI GGATG 5 cut(s) 649, 773, 853, 1411, 1822
FriOI GRGCYC 1 cut(s) 1616
Fsp4HI GCNGC 7 cut(s) 6, 139, 262, 387, 1394, 1656, 1814
FspBI CTAG 7 cut(s) 390, 600, 957, 1028, 1490, 1595, 2072
FspI TGCGCA 1 cut(s) 1916
GlaI GCGC 2 cut(s) 822, 1916
GluI GCNGC 7 cut(s) 6, 139, 262, 387, 1394, 1656, 1814
GsaI CCCAGC 1 cut(s) 1479
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 4 cut(s) 8, 305, 366, 1449
HapII CCGG 6 cut(s) 22, 367, 373, 448, 758, 2035
HgaI GACGC 1 cut(s) 150
HhaI GCGC 2 cut(s) 823, 1917
Hin1I GRCGYC 1 cut(s) 161
Hin1II CATG 7 cut(s) 160, 186, 349, 706, 1447, 1820, 1893
Hin6I GCGC 2 cut(s) 821, 1915
HinP1I GCGC 2 cut(s) 821, 1915
HindIII AAGCTT 2 cut(s) 509, 1982
HinfI GANTC 8 cut(s) 308, 376, 500, 865, 1190, 1538, 1591, 1685
HpaII CCGG 6 cut(s) 22, 367, 373, 448, 758, 2035
HphI GGTGA 3 cut(s) 844, 1079, 2039
Hpy166II GTNNAC 3 cut(s) 1092, 1310, 2140
Hpy188I TCNGA 6 cut(s) 47, 62, 112, 870, 1171, 1189
Hpy188III TCNNGA 6 cut(s) 497, 1495, 1520, 1595, 1988, 2157
Hpy8I GTNNAC 3 cut(s) 1092, 1310, 2140
Hpy99I CGWCG 1 cut(s) 166
HpyCH4III ACNGT 3 cut(s) 40, 244, 1454
HpyCH4IV ACGT 2 cut(s) 665, 1088
HpyCH4V TGCA 7 cut(s) 1111, 1122, 1127, 1583, 1672, 1816, 2081
HpyF10VI GCNNNNNNNGC 2 cut(s) 658, 1006
HpyF3I CTNAG 8 cut(s) 59, 101, 609, 1135, 1192, 1251, 1368, 1502
HpySE526I ACGT 2 cut(s) 665, 1088
Hsp92I GRCGYC 1 cut(s) 161
Hsp92II CATG 7 cut(s) 160, 186, 349, 706, 1447, 1820, 1893
HspAI GCGC 2 cut(s) 821, 1915
Ksp22I TGATCA 1 cut(s) 1440
Kzo9I GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
LguI GCTCTTC 1 cut(s) 1604
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 4 cut(s) 150, 1405, 1667, 1800
LweI GCATC 6 cut(s) 103, 273, 1098, 1488, 1593, 1800
MaeI CTAG 7 cut(s) 390, 600, 957, 1028, 1490, 1595, 2072
MaeII ACGT 2 cut(s) 665, 1088
MaeIII GTNAC 5 cut(s) 646, 766, 877, 1173, 2027
MalI GATC 6 cut(s) 1037, 1299, 1419, 1442, 1752, 2089
MboI GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
MfeI CAATTG 1 cut(s) 408
MflI RGATCY 2 cut(s) 1417, 1750
MhlI GDGCHC 2 cut(s) 1616, 1681
MluCI AATT 4 cut(s) 53, 290, 408, 2144
MlyI GAGTC 3 cut(s) 509, 1184, 1585
MmeI TCCRAC 3 cut(s) 201, 1539, 1692
Mph1103I ATGCAT 2 cut(s) 1113, 1674
MroXI GAANNNNTTC 1 cut(s) 1154
MslI CAYNNNNRTG 4 cut(s) 1106, 1122, 1163, 1821
MspA1I CMGCKG 1 cut(s) 2134
MspCI CTTAAG 1 cut(s) 584
MspI CCGG 6 cut(s) 22, 367, 373, 448, 758, 2035
MspR9I CCNGG 1 cut(s) 22
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 2 cut(s) 1111, 2124
MvnI CGCG 1 cut(s) 823
MwoI GCNNNNNNNGC 2 cut(s) 658, 1006
NciI CCSGG 1 cut(s) 22
NcoI CCATGG 3 cut(s) 156, 182, 702
NdeII GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
NlaIII CATG 7 cut(s) 160, 186, 349, 706, 1447, 1820, 1893
NlaIV GGNNCC 2 cut(s) 371, 1182
NmuCI GTSAC 1 cut(s) 2027
NsbI TGCGCA 1 cut(s) 1916
NsiI ATGCAT 2 cut(s) 1113, 1674
NspI RCATGY 1 cut(s) 1893
NspV TTCGAA 1 cut(s) 1470
PaeR7I CTCGAG 1 cut(s) 440
PciSI GCTCTTC 1 cut(s) 1604
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PctI GAATGC 2 cut(s) 1111, 2124
PdmI GAANNNNTTC 1 cut(s) 1154
PfeI GAWTC 5 cut(s) 308, 376, 865, 1538, 1685
PflFI GACNNNGTC 1 cut(s) 2105
PflMI CCANNNNNTGG 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 447
PkrI GCNGC 7 cut(s) 7, 140, 263, 388, 1395, 1657, 1815
PleI GAGTC 3 cut(s) 508, 1184, 1585
PpsI GAGTC 3 cut(s) 508, 1184, 1585
Ppu21I YACGTR 1 cut(s) 1089
PpuMI RGGWCCY 2 cut(s) 105, 1180
PsiI TTATAA 1 cut(s) 774
Psp124BI GAGCTC 1 cut(s) 1616
Psp5II RGGWCCY 2 cut(s) 105, 1180
PspEI GGTNACC 1 cut(s) 2027
PspFI CCCAGC 1 cut(s) 1475
PspN4I GGNNCC 2 cut(s) 371, 1182
PspPI GGNCC 6 cut(s) 105, 303, 420, 445, 1180, 1447
PspPPI RGGWCCY 2 cut(s) 105, 1180
PspXI VCTCGAGB 1 cut(s) 440
PstNI CAGNNNCTG 1 cut(s) 1399
PsuI RGATCY 2 cut(s) 1417, 1750
PsyI GACNNNGTC 1 cut(s) 2105
PvuII CAGCTG 1 cut(s) 2134
RsaI GTAC 5 cut(s) 1093, 1309, 1346, 1481, 2006
RsaNI GTAC 5 cut(s) 1092, 1308, 1345, 1480, 2005
RseI CAYNNNNRTG 4 cut(s) 1106, 1122, 1163, 1821
SacI GAGCTC 1 cut(s) 1616
SapI GCTCTTC 1 cut(s) 1604
SatI GCNGC 7 cut(s) 6, 139, 262, 387, 1394, 1656, 1814
Sau3AI GATC 6 cut(s) 1035, 1297, 1417, 1440, 1750, 2087
Sau96I GGNCC 6 cut(s) 105, 303, 420, 445, 1180, 1447
ScaI AGTACT 1 cut(s) 1346
SchI GAGTC 3 cut(s) 509, 1184, 1585
ScrFI CCNGG 1 cut(s) 22
SduI GDGCHC 2 cut(s) 1616, 1681
SfaNI GCATC 6 cut(s) 103, 273, 1098, 1488, 1593, 1800
SfcI CTRYAG 1 cut(s) 1453
Sfr274I CTCGAG 1 cut(s) 440
SfuI TTCGAA 1 cut(s) 1470
SinI GGWCC 4 cut(s) 105, 420, 445, 1180
SlaI CTCGAG 1 cut(s) 440
SmiMI CAYNNNNRTG 4 cut(s) 1106, 1122, 1163, 1821
SmlI CTYRAG 3 cut(s) 353, 440, 584
SmoI CTYRAG 3 cut(s) 353, 440, 584
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 4 cut(s) 53, 290, 408, 2144
SsiI CCGC 6 cut(s) 5, 70, 147, 259, 262, 387
SspI AATATT 3 cut(s) 673, 1735, 1899
SspMI CTAG 7 cut(s) 390, 600, 957, 1028, 1490, 1595, 2072
SstI GAGCTC 1 cut(s) 1616
StyD4I CCNGG 1 cut(s) 20
StyI CCWWGG 4 cut(s) 133, 156, 182, 702
TaaI ACNGT 3 cut(s) 40, 244, 1454
TaiI ACGT 2 cut(s) 668, 1091
TaqI TCGA 6 cut(s) 441, 530, 575, 1470, 1987, 2156
TasI AATT 4 cut(s) 53, 290, 408, 2144
TatI WGTACW 4 cut(s) 1091, 1307, 1344, 2004
TauI GCSGC 3 cut(s) 8, 264, 389
TfiI GAWTC 5 cut(s) 308, 376, 865, 1538, 1685
TscAI CASTG 3 cut(s) 1110, 1457, 1777
TseFI GTSAC 1 cut(s) 2027
TseI GCWGC 4 cut(s) 138, 1393, 1655, 1813
Tsp45I GTSAC 1 cut(s) 2027
TspDTI ATGAA 7 cut(s) 334, 539, 639, 710, 710, 1395, 2025
TspRI CASTG 3 cut(s) 1110, 1457, 1777
Tth111I GACNNNGTC 1 cut(s) 2105
Van91I CCANNNNNTGG 1 cut(s) 183
Vha464I CTTAAG 1 cut(s) 584
VpaK11BI GGWCC 4 cut(s) 105, 420, 445, 1180
XapI RAATTY 2 cut(s) 290, 2144
XbaI TCTAGA 1 cut(s) 1594
XceI RCATGY 1 cut(s) 1893
XhoI CTCGAG 1 cut(s) 440
XmnI GAANNNNTTC 1 cut(s) 1154
XspI CTAG 7 cut(s) 390, 600, 957, 1028, 1490, 1595, 2072
ZrmI AGTACT 1 cut(s) 1346
Zsp2I ATGCAT 2 cut(s) 1113, 1674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.