Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0005468

Physical Location & Seq

Genomic Coordinates Forward (+)

38517779 .. 38520728

Gene Structure 2950 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG194800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

ATGGCCGCCAAACCCATTTCCCGGAGACCAGCCTTGACAGTTCTCCGAGAGCAATTCCTCAGACCCAACCGCAACTCCAAACCTTCTCCTCCTCCTCCTCTCAGGTCCTCCGATGCTGAAAAGCCCAAATCCCAAGGAAGCAAAACCGCCAAAGCCGTGGCGTCGCTCATCAACTCCAACCCATGGTCATCTCGCCTCCAGTCTTCTCTCTCTTCTCTCTCTCCCTCCATCTCCACCACCACCGTCTCCCAAACCCTCCGCCGCATCAAAACCCCAACCCAAGCCCTCAAATTCTTCAACTGGGCCGAAGCAATGGGCTTCTCCCACAACCCCCAATCCTTCTTCATGTTGCTTGAGATTTTAGGCCGGAACCGGAATCTCAATGCCGCTAGAAATCTCTTGTTTTCGATTGAGAAGAGGTCCAATGGGAAGGTTAAGCTCGAGGACCGGTTTTTTAATAGCTTGATTAGGAGCTATGGGGAAGCAGGGTTGTTCCAGGAGTCTGTAAAGCTTTTTGGGACGATGAAGTCGATGGGGATATCGGCTTCTGTTGTTAGTTTCAATAGTTTGCTTTCGATTTTGCTTAAGAGGGGTAGGACTAGTAGGGCTAAGAATGTTTATGATGAAATGCTTGGGATGTATGGAGTTACGCCGGATACGCATACGTTTAATATTTTGATTAGGGGGTTCTGTATGAACTCCATGGTTGATGAGGGGTTTCATTATTTTAAGGAAATGTCACGGTTGAAATGTGAGCCGGATGTGGTTACTTATAATACGATTGTGGATGGGTTGTGTAGAGCAGGGAAGGTTGAGATTGCGCGAAATGTGGTGAAGGGTATGAGGAAGAGAAGTGGGGAGTTGAATCCGAATGTTGTTACTTATACGACATTGATTAGAGGGTATTGTGTGAAAGAAGATGTTGATGAGGCTTTGTGTGTTTTGGAGGAGATGACTAGTCAAGGGTTGAAACCAAATGAAATTACCTATAATACTCTGCTTAAAGGGCTTAGTGAAACAAAGAAGCTAGATAAGGTAAAAGAAGTCTTGGAGGGAACAATGAGAGGTGAGGGATTTACTCCTGATACGTGTACGTTCAACATGCTGATGCATTCGCATTGCAATGCAGGAAACTTACAGGAAGCATTGAAGGTTTTCACAATGATGTCAGAGTTACAGGTCCCTCCAGATTCAGCTACATACAGCATTTTGATACGCAGTTTGTGTCAGCGAAGAGATTATGGTAAAGCTGAGGAGTTGTTTGATGAGCTATCCAAGAAACAAATCTTGTTAAGTGATCATGGCTGTACACCTGTTGTTGCCTCATACAAACCTATCTTTGAGTACTTATGTAGTAATGGGAGAACTAAGAAAGCAGATGAAGTGTTTAGGCAGCTACTGAAAAGAGGAACACAAGATCCTCCATCCTACAAGACTTTGATCATGGGCCACTGTAGGGAAGGCACATTCGAAGCTGGGTACAAACTTCTAGTCTTGATGCTTAGAAGGGATTTTGTTCCTGATGTTGAGATTTATGATTCCTTGATTGATGGTCTGTTGGAAAAGGGTAAGCCCCTTCTTGCACAACAGACTCTAGAGAAGATGCTGAAGAGCTCTCATCTCCCAAAAACATCTACTTTCCATTCTATACTGGCAGCTCTTTTAGAAATGCATTGTGCCCGTGAATCTGCCAGCTTTGTTATTTTAATGTTGGAGAAAAACATTAGACAAAATATTAACCTCTCAACAGATCTCGTAAGGCTTCTGTTCAGTGAGAGATTGCGAGATAAAGCATTAGGGATGCTGCATGATAATGGATACTTGATTAAAATAGAAGAAGTGGTCAGTTTCCTTTACCAGAGCAGAAAGTTAATAGAGGCATGTGAAATATTGCGATTTAGTCTGCGCAAGCAAGCAAATGTCAGTATTGATATATTTGACCAAGTTCTATTAGGGCTATGTGATATCAATAAGCTTCGAGAAGCATTTGGCTTGTACTATGAACTGATTGAGAATGGCGACCACCAGCAGTTGCCGTGCTTACATCATTTAAAGTCTTCCCTAGAGGTTGCAGGTAGATCAGTTGAAGCTGACTTTGTCTCTAAAAGAATGCCAAAACATCAGCTGGTGGACAAATTTGGGAAGTCGAGACCTCAACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Chal_sti_synt_C Chal_sti_synt_N PPR PPR_1 PPR_2 PPR_3

Protein Analysis

720

Amino Acids

81.4

Weight (kDa)

9.27

Isoelectric Point (pI)

47.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	183 - 231	1.3e-12	PPR repeat family
PPR_long	PF17177	202 - 353	8.2e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	215 - 247	3.8e-08	PPR repeat
PPR_2	PF13041	218 - 267	3.6e-16	PPR repeat family
PPR	PF01535	222 - 251	8.4e-06	PPR repeat
PPR_3	PF13812	243 - 301	1.6e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	251 - 282	2.1e-12	PPR repeat
PPR_2	PF13041	253 - 286	1.5e-07	PPR repeat family
TPR_24	PF23276	267 - 351	7.8e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	288 - 319	5.3e-11	PPR repeat
PPR_3	PF13812	288 - 335	7.7e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	290 - 338	3.1e-18	PPR repeat family
PPR	PF01535	293 - 323	1.1e-07	PPR repeat
PPR_3	PF13812	314 - 371	1e-10	Pentatricopeptide repeat domain
PPR_3	PF13812	354 - 406	5.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	358 - 389	3e-09	PPR repeat
PPR_2	PF13041	361 - 410	6.1e-16	PPR repeat family
PPR_1	PF12854	394 - 423	7.5e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005468)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02060
fragaria_vesca	FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470 FvH4_7g09470
malus_domestica	MD02G1218600.v1.1
prunus_persica	Prupe.2G122500_v2.0.a1
pyrus_communis	pycom12652g00050
rosa_chinensis	RchiOBHm_Chr1g0346171
rosa_laevigata	RLG00000028810
rosa_multiflora	Rmu_sc0003809.1_g000024
rosa_roxburghii	Rroxscaffold_4G00308800
rosa_rugosa	Rorug01G0178600
rosa_samantha	Rh1AG197700 Rh1BG162900 Rh1BG163000 Rh1BG163800 Rh1CG182800 Rh1DG193600 Rh1DG193700 Rh1DG194800
rosa_wichuraiana	Rw1G016310 Rw1G016590 Rw1G016620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	774
AasI	GACNNNNNNGTC	1 cut(s)	526
Acc16I	TGCGCA	1 cut(s)	1907
Acc36I	ACCTGC	1 cut(s)	2063
AccB7I	CCANNNNNTGG	1 cut(s)	183
AccII	CGCG	1 cut(s)	823
AciI	CCGC	6 cut(s)	6, 70, 147, 259, 262, 387
AclWI	GGATC	1 cut(s)	1412
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	290, 2135
AcuI	CTGAAG	1 cut(s)	1628
AcyI	GRCGYC	1 cut(s)	161
AfaI	GTAC	5 cut(s)	1093, 1309, 1346, 1481, 1997
AfiI	CCNNNNNNNGG	3 cut(s)	21, 183, 1456
AflII	CTTAAG	1 cut(s)	584
AflIII	ACRYGT	1 cut(s)	1088
AgeI	ACCGGT	1 cut(s)	447
AgsI	TTSAA	8 cut(s)	298, 562, 748, 865, 970, 1099, 1150, 2087
AhlI	ACTAGT	2 cut(s)	599, 956
AjnI	CCWGG	1 cut(s)	495
AloI	GAACNNNNNNTCC	2 cut(s)	1402, 1434
Alw21I	GWGCWC	1 cut(s)	1616
Alw26I	GTCTC	4 cut(s)	19, 250, 2104, 2143
AlwI	GGATC	1 cut(s)	1412
AlwNI	CAGNNNCTG	1 cut(s)	1399
Ama87I	CYCGRG	1 cut(s)	440
AoxI	GGCC	4 cut(s)	3, 303, 364, 1447
ApeKI	GCWGC	3 cut(s)	1393, 1655, 1804
ApoI	RAATTY	2 cut(s)	290, 2135
ArsI	GACNNNNNNTTYG	2 cut(s)	1476, 1508
AsiGI	ACCGGT	1 cut(s)	447
Asp700I	GAANNNNTTC	1 cut(s)	1154
AspLEI	GCGC	2 cut(s)	823, 1908
AspS9I	GGNCC	6 cut(s)	105, 303, 420, 445, 1180, 1447
AsuC2I	CCSGG	1 cut(s)	22
AsuHPI	GGTGA	2 cut(s)	844, 1079
AsuII	TTCGAA	1 cut(s)	1470
AvaI	CYCGRG	1 cut(s)	440
AvaII	GGWCC	4 cut(s)	105, 420, 445, 1180
BaeGI	GKGCMC	1 cut(s)	1681
BanII	GRGCYC	1 cut(s)	1616
BbsI	GAAGAC	2 cut(s)	195, 2049
Bbv12I	GWGCWC	1 cut(s)	1616
BbvCI	CCTCAGC	1 cut(s)	1251
BbvI	GCAGC	3 cut(s)	1405, 1667, 1791
BccI	CCATC	5 cut(s)	236, 526, 782, 1432, 1544
BceAI	ACGGC	2 cut(s)	140, 2020
BciT130I	CCWGG	1 cut(s)	497
BciVI	GTATCC	2 cut(s)	649, 1811
BclI	TGATCA	2 cut(s)	1297, 1440
BcnI	CCSGG	1 cut(s)	22
BcoDI	GTCTC	4 cut(s)	19, 250, 2104, 2143
BcuI	ACTAGT	2 cut(s)	599, 956
BfaI	CTAG	7 cut(s)	390, 600, 957, 1028, 1490, 1595, 2063
BfmI	CTRYAG	1 cut(s)	1453
BfrI	CTTAAG	1 cut(s)	584
BfuAI	ACCTGC	1 cut(s)	2063
BfuI	GTATCC	2 cut(s)	649, 1811
BglII	AGATCT	1 cut(s)	1750
BisI	GCNGC	6 cut(s)	6, 262, 387, 1394, 1656, 1805
BlsI	GCNGC	6 cut(s)	7, 263, 388, 1395, 1657, 1806
BmcAI	AGTACT	1 cut(s)	1346
Bme1390I	CCNGG	2 cut(s)	22, 497
Bme18I	GGWCC	4 cut(s)	105, 420, 445, 1180
BmeT110I	CYCGRG	1 cut(s)	440
BmgT120I	GGNCC	6 cut(s)	105, 303, 420, 445, 1180, 1447
BmiI	GGNNCC	2 cut(s)	371, 1182
BmrFI	CCNGG	2 cut(s)	22, 497
BmrI	ACTGGG	1 cut(s)	310
BmsI	GCATC	6 cut(s)	103, 273, 1098, 1488, 1593, 1791
BmuI	ACTGGG	1 cut(s)	310
BpiI	GAAGAC	2 cut(s)	195, 2049
BpmI	CTGGAG	2 cut(s)	182, 1170
Bpu10I	CCTNAGC	1 cut(s)	1251
Bpu14I	TTCGAA	1 cut(s)	1470
BpuEI	CTTGAG	1 cut(s)	374
BpuMI	CCSGG	1 cut(s)	22
BsaAI	YACGTR	1 cut(s)	1089
BsaHI	GRCGYC	1 cut(s)	161
BsaI	GGTCTC	2 cut(s)	19, 2143
BsaJI	CCNNGG	4 cut(s)	133, 156, 182, 702
BsaWI	WCCGGW	2 cut(s)	372, 447
BsaXI	ACNNNNNCTCC	2 cut(s)	59, 89
Bsc4I	CCNNNNNNNGG	3 cut(s)	21, 183, 1456
Bse118I	RCCGGY	1 cut(s)	447
Bse1I	ACTGG	3 cut(s)	199, 305, 1656
Bse3DI	GCAATG	3 cut(s)	318, 1117, 1129
BseBI	CCWGG	1 cut(s)	497
BseDI	CCNNGG	4 cut(s)	133, 156, 182, 702
BseGI	GGATG	5 cut(s)	642, 766, 793, 1424, 1806
BseLI	CCNNNNNNNGG	3 cut(s)	21, 183, 1456
BseMI	GCAATG	3 cut(s)	318, 1117, 1129
BseMII	CTCAG	3 cut(s)	73, 115, 1242
BseNI	ACTGG	3 cut(s)	199, 305, 1656
BseRI	GAGGAG	6 cut(s)	78, 81, 84, 87, 962, 1268
BseSI	GKGCMC	1 cut(s)	1681
BseXI	GCAGC	3 cut(s)	1405, 1667, 1791
BseYI	CCCAGC	1 cut(s)	1475
Bsh1236I	CGCG	1 cut(s)	823
BshFI	GGCC	4 cut(s)	5, 305, 366, 1449
BshTI	ACCGGT	1 cut(s)	447
BsiHKAI	GWGCWC	1 cut(s)	1616
BsiHKCI	CYCGRG	1 cut(s)	440
BsiSI	CCGG	6 cut(s)	22, 367, 373, 448, 653, 758
BslFI	GGGAC	2 cut(s)	532, 1166
BslI	CCNNNNNNNGG	3 cut(s)	21, 183, 1456
BsmAI	GTCTC	4 cut(s)	19, 250, 2104, 2143
BsmBI	CGTCTC	1 cut(s)	250
BsmFI	GGGAC	2 cut(s)	532, 1166
BsmI	GAATGC	2 cut(s)	1111, 2115
BsnI	GGCC	4 cut(s)	5, 305, 366, 1449
Bso31I	GGTCTC	2 cut(s)	19, 2143
BsoBI	CYCGRG	1 cut(s)	440
Bsp119I	TTCGAA	1 cut(s)	1470
Bsp1286I	GDGCHC	2 cut(s)	1616, 1681
Bsp1407I	TGTACA	1 cut(s)	1307
Bsp143I	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
Bsp19I	CCATGG	2 cut(s)	182, 702
BspACI	CCGC	6 cut(s)	6, 70, 147, 259, 262, 387
BspANI	GGCC	4 cut(s)	5, 305, 366, 1449
BspCNI	CTCAG	3 cut(s)	72, 114, 1243
BspFNI	CGCG	1 cut(s)	823
BspLI	GGNNCC	2 cut(s)	371, 1182
BspMI	ACCTGC	1 cut(s)	2063
BspPI	GGATC	1 cut(s)	1412
BspQI	GCTCTTC	1 cut(s)	1604
BspT104I	TTCGAA	1 cut(s)	1470
BspTI	CTTAAG	1 cut(s)	584
BspTNI	GGTCTC	2 cut(s)	19, 2143
BsrDI	GCAATG	3 cut(s)	318, 1117, 1129
BsrFI	RCCGGY	1 cut(s)	447
BsrGI	TGTACA	1 cut(s)	1307
BsrI	ACTGG	3 cut(s)	199, 305, 1656
BssAI	RCCGGY	1 cut(s)	447
BssECI	CCNNGG	4 cut(s)	133, 156, 182, 702
BssMI	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
BssNI	GRCGYC	1 cut(s)	161
BssT1I	CCWWGG	3 cut(s)	133, 182, 702
Bst2UI	CCWGG	1 cut(s)	497
Bst4CI	ACNGT	4 cut(s)	40, 244, 744, 1454
Bst6I	CTCTTC	5 cut(s)	217, 410, 842, 1228, 1604
BstACI	GRCGYC	1 cut(s)	161
BstAFI	CTTAAG	1 cut(s)	584
BstAUI	TGTACA	1 cut(s)	1307
BstBAI	YACGTR	1 cut(s)	1089
BstBI	TTCGAA	1 cut(s)	1470
BstC8I	GCNNGC	3 cut(s)	1693, 1910, 1914
BstDEI	CTNAG	7 cut(s)	59, 101, 609, 1010, 1251, 1368, 1502
BstDSI	CCRYGG	3 cut(s)	156, 182, 702
BstF5I	GGATG	5 cut(s)	642, 766, 793, 1424, 1806
BstFNI	CGCG	1 cut(s)	823
BstHHI	GCGC	2 cut(s)	823, 1908
BstKTI	GATC	5 cut(s)	1300, 1420, 1443, 1753, 2081
BstMAI	GTCTC	4 cut(s)	19, 250, 2104, 2143
BstMBI	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
BstMWI	GCNNNNNNNGC	2 cut(s)	658, 1006
BstNI	CCWGG	1 cut(s)	497
BstNSI	RCATGY	2 cut(s)	1105, 1884
BstSCI	CCNGG	2 cut(s)	20, 495
BstSFI	CTRYAG	1 cut(s)	1453
BstSLI	GKGCMC	1 cut(s)	1681
BstUI	CGCG	1 cut(s)	823
BstV1I	GCAGC	3 cut(s)	1405, 1667, 1791
BstV2I	GAAGAC	2 cut(s)	195, 2049
BstX2I	RGATCY	2 cut(s)	1417, 1750
BstXI	CCANNNNNNTGG	1 cut(s)	157
BstYI	RGATCY	2 cut(s)	1417, 1750
BsuI	GTATCC	2 cut(s)	649, 1811
BsuRI	GGCC	4 cut(s)	5, 305, 366, 1449
BtgI	CCRYGG	3 cut(s)	156, 182, 702
BtsCI	GGATG	5 cut(s)	642, 766, 793, 1424, 1806
BtsIMutI	CAGTG	2 cut(s)	1450, 1777
BveI	ACCTGC	1 cut(s)	2063
Cac8I	GCNNGC	3 cut(s)	1693, 1910, 1914
CaiI	CAGNNNCTG	1 cut(s)	1399
CfoI	GCGC	2 cut(s)	823, 1908
Cfr10I	RCCGGY	1 cut(s)	447
Cfr13I	GGNCC	6 cut(s)	105, 303, 420, 445, 1180, 1447
CseI	GACGC	1 cut(s)	150
Csp6I	GTAC	5 cut(s)	1092, 1308, 1345, 1480, 1996
CspAI	ACCGGT	1 cut(s)	447
CviAII	CATG	8 cut(s)	183, 346, 703, 1102, 1301, 1444, 1808, 1881
CviQI	GTAC	5 cut(s)	1092, 1308, 1345, 1480, 1996
DdeI	CTNAG	7 cut(s)	59, 101, 609, 1010, 1251, 1368, 1502
DpnI	GATC	5 cut(s)	1299, 1419, 1442, 1752, 2080
DpnII	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
DraI	TTTAAA	1 cut(s)	2052
DrdI	GACNNNNNNGTC	1 cut(s)	526
DseDI	GACNNNNNNGTC	1 cut(s)	526
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	5 cut(s)	217, 410, 842, 1228, 1604
EarI	CTCTTC	5 cut(s)	217, 410, 842, 1228, 1604
EciI	GGCGGA	1 cut(s)	248
Ecl136II	GAGCTC	1 cut(s)	1614
Eco130I	CCWWGG	3 cut(s)	133, 182, 702
Eco24I	GRGCYC	1 cut(s)	1616
Eco31I	GGTCTC	2 cut(s)	19, 2143
Eco32I	GATATC	2 cut(s)	540, 1966
Eco47I	GGWCC	4 cut(s)	105, 420, 445, 1180
Eco53kI	GAGCTC	1 cut(s)	1614
Eco57I	CTGAAG	1 cut(s)	1628
Eco88I	CYCGRG	1 cut(s)	440
EcoICRI	GAGCTC	1 cut(s)	1614
EcoO109I	RGGNCCY	2 cut(s)	105, 1180
EcoRII	CCWGG	1 cut(s)	495
EcoRV	GATATC	2 cut(s)	540, 1966
EcoT14I	CCWWGG	3 cut(s)	133, 182, 702
EcoT22I	ATGCAT	2 cut(s)	1113, 1674
EcoT38I	GRGCYC	1 cut(s)	1616
ErhI	CCWWGG	3 cut(s)	133, 182, 702
Esp3I	CGTCTC	1 cut(s)	250
FaeI	CATG	8 cut(s)	186, 349, 706, 1105, 1304, 1447, 1811, 1884
FaqI	GGGAC	2 cut(s)	532, 1166
FatI	CATG	8 cut(s)	182, 345, 702, 1101, 1300, 1443, 1807, 1880
FbaI	TGATCA	2 cut(s)	1297, 1440
Fnu4HI	GCNGC	6 cut(s)	6, 262, 387, 1394, 1656, 1805
FokI	GGATG	5 cut(s)	649, 773, 800, 1411, 1813
FriOI	GRGCYC	1 cut(s)	1616
Fsp4HI	GCNGC	6 cut(s)	6, 262, 387, 1394, 1656, 1805
FspBI	CTAG	7 cut(s)	390, 600, 957, 1028, 1490, 1595, 2063
FspI	TGCGCA	1 cut(s)	1907
GlaI	GCGC	2 cut(s)	822, 1907
GluI	GCNGC	6 cut(s)	6, 262, 387, 1394, 1656, 1805
GsaI	CCCAGC	1 cut(s)	1479
GsuI	CTGGAG	2 cut(s)	182, 1170
HaeIII	GGCC	4 cut(s)	5, 305, 366, 1449
HapII	CCGG	6 cut(s)	22, 367, 373, 448, 653, 758
HgaI	GACGC	1 cut(s)	150
HhaI	GCGC	2 cut(s)	823, 1908
Hin1I	GRCGYC	1 cut(s)	161
Hin1II	CATG	8 cut(s)	186, 349, 706, 1105, 1304, 1447, 1811, 1884
Hin6I	GCGC	2 cut(s)	821, 1906
HinP1I	GCGC	2 cut(s)	821, 1906
HindIII	AAGCTT	2 cut(s)	509, 1973
HinfI	GANTC	7 cut(s)	376, 500, 865, 1190, 1538, 1591, 1685
HpaII	CCGG	6 cut(s)	22, 367, 373, 448, 653, 758
HphI	GGTGA	2 cut(s)	844, 1079
Hpy166II	GTNNAC	3 cut(s)	1092, 1310, 2131
Hpy188I	TCNGA	5 cut(s)	47, 62, 112, 870, 1171
Hpy188III	TCNNGA	7 cut(s)	1082, 1187, 1495, 1520, 1595, 1979, 2148
Hpy8I	GTNNAC	3 cut(s)	1092, 1310, 2131
Hpy99I	CGWCG	1 cut(s)	166
HpyAV	CCTTC	9 cut(s)	93, 349, 424, 802, 829, 1144, 1454, 1500, 1586
HpyCH4III	ACNGT	4 cut(s)	40, 244, 744, 1454
HpyCH4IV	ACGT	3 cut(s)	665, 1088, 1094
HpyCH4V	TGCA	7 cut(s)	1111, 1122, 1127, 1583, 1672, 1807, 2072
HpyF10VI	GCNNNNNNNGC	2 cut(s)	658, 1006
HpyF3I	CTNAG	7 cut(s)	59, 101, 609, 1010, 1251, 1368, 1502
HpySE526I	ACGT	3 cut(s)	665, 1088, 1094
Hsp92I	GRCGYC	1 cut(s)	161
Hsp92II	CATG	8 cut(s)	186, 349, 706, 1105, 1304, 1447, 1811, 1884
HspAI	GCGC	2 cut(s)	821, 1906
Ksp22I	TGATCA	2 cut(s)	1297, 1440
Kzo9I	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
LguI	GCTCTTC	1 cut(s)	1604
LmnI	GCTCC	1 cut(s)	471
Lsp1109I	GCAGC	3 cut(s)	1405, 1667, 1791
LweI	GCATC	6 cut(s)	103, 273, 1098, 1488, 1593, 1791
MaeI	CTAG	7 cut(s)	390, 600, 957, 1028, 1490, 1595, 2063
MaeII	ACGT	3 cut(s)	665, 1088, 1094
MaeIII	GTNAC	5 cut(s)	646, 738, 766, 877, 1173
MalI	GATC	5 cut(s)	1299, 1419, 1442, 1752, 2080
MboI	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
MflI	RGATCY	2 cut(s)	1417, 1750
MhlI	GDGCHC	2 cut(s)	1616, 1681
MluCI	AATT	4 cut(s)	53, 290, 981, 2135
MlyI	GAGTC	2 cut(s)	509, 1585
MmeI	TCCRAC	3 cut(s)	201, 1539, 1692
Mph1103I	ATGCAT	2 cut(s)	1113, 1674
MroXI	GAANNNNTTC	1 cut(s)	1154
MslI	CAYNNNNRTG	4 cut(s)	1106, 1122, 1163, 1812
MspA1I	CMGCKG	1 cut(s)	2125
MspCI	CTTAAG	1 cut(s)	584
MspI	CCGG	6 cut(s)	22, 367, 373, 448, 653, 758
MspR9I	CCNGG	2 cut(s)	22, 497
Mva1269I	GAATGC	2 cut(s)	1111, 2115
MvaI	CCWGG	1 cut(s)	497
MvnI	CGCG	1 cut(s)	823
MwoI	GCNNNNNNNGC	2 cut(s)	658, 1006
NciI	CCSGG	1 cut(s)	22
NcoI	CCATGG	2 cut(s)	182, 702
NdeII	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
NlaIII	CATG	8 cut(s)	186, 349, 706, 1105, 1304, 1447, 1811, 1884
NlaIV	GGNNCC	2 cut(s)	371, 1182
NmuCI	GTSAC	1 cut(s)	738
NsbI	TGCGCA	1 cut(s)	1907
NsiI	ATGCAT	2 cut(s)	1113, 1674
NspI	RCATGY	2 cut(s)	1105, 1884
NspV	TTCGAA	1 cut(s)	1470
PaeR7I	CTCGAG	1 cut(s)	440
PciSI	GCTCTTC	1 cut(s)	1604
PcsI	WCGNNNNNNNCGW	1 cut(s)	527
PctI	GAATGC	2 cut(s)	1111, 2115
PdmI	GAANNNNTTC	1 cut(s)	1154
PfeI	GAWTC	5 cut(s)	376, 865, 1190, 1538, 1685
PflFI	GACNNNGTC	1 cut(s)	2096
PflMI	CCANNNNNTGG	1 cut(s)	183
PfoI	TCCNGGA	2 cut(s)	20, 495
PinAI	ACCGGT	1 cut(s)	447
PkrI	GCNGC	6 cut(s)	7, 263, 388, 1395, 1657, 1806
PleI	GAGTC	2 cut(s)	508, 1585
PpsI	GAGTC	2 cut(s)	508, 1585
Ppu21I	YACGTR	1 cut(s)	1089
PpuMI	RGGWCCY	2 cut(s)	105, 1180
PsiI	TTATAA	1 cut(s)	774
Psp124BI	GAGCTC	1 cut(s)	1616
Psp5II	RGGWCCY	2 cut(s)	105, 1180
Psp6I	CCWGG	1 cut(s)	495
PspFI	CCCAGC	1 cut(s)	1475
PspGI	CCWGG	1 cut(s)	495
PspN4I	GGNNCC	2 cut(s)	371, 1182
PspPI	GGNCC	6 cut(s)	105, 303, 420, 445, 1180, 1447
PspPPI	RGGWCCY	2 cut(s)	105, 1180
PspXI	VCTCGAGB	1 cut(s)	440
PstNI	CAGNNNCTG	1 cut(s)	1399
PsuI	RGATCY	2 cut(s)	1417, 1750
PsyI	GACNNNGTC	1 cut(s)	2096
PvuII	CAGCTG	1 cut(s)	2125
RsaI	GTAC	5 cut(s)	1093, 1309, 1346, 1481, 1997
RsaNI	GTAC	5 cut(s)	1092, 1308, 1345, 1480, 1996
RseI	CAYNNNNRTG	4 cut(s)	1106, 1122, 1163, 1812
SacI	GAGCTC	1 cut(s)	1616
SapI	GCTCTTC	1 cut(s)	1604
SatI	GCNGC	6 cut(s)	6, 262, 387, 1394, 1656, 1805
Sau3AI	GATC	5 cut(s)	1297, 1417, 1440, 1750, 2078
Sau96I	GGNCC	6 cut(s)	105, 303, 420, 445, 1180, 1447
ScaI	AGTACT	1 cut(s)	1346
SchI	GAGTC	2 cut(s)	509, 1585
ScrFI	CCNGG	2 cut(s)	22, 497
SduI	GDGCHC	2 cut(s)	1616, 1681
SfaNI	GCATC	6 cut(s)	103, 273, 1098, 1488, 1593, 1791
SfcI	CTRYAG	1 cut(s)	1453
Sfr274I	CTCGAG	1 cut(s)	440
SfuI	TTCGAA	1 cut(s)	1470
SinI	GGWCC	4 cut(s)	105, 420, 445, 1180
SlaI	CTCGAG	1 cut(s)	440
SmiMI	CAYNNNNRTG	4 cut(s)	1106, 1122, 1163, 1812
SmlI	CTYRAG	3 cut(s)	353, 440, 584
SmoI	CTYRAG	3 cut(s)	353, 440, 584
SpeI	ACTAGT	2 cut(s)	599, 956
Sse9I	AATT	4 cut(s)	53, 290, 981, 2135
SsiI	CCGC	6 cut(s)	6, 70, 147, 259, 262, 387
SspI	AATATT	3 cut(s)	673, 1735, 1890
SspMI	CTAG	7 cut(s)	390, 600, 957, 1028, 1490, 1595, 2063
SstI	GAGCTC	1 cut(s)	1616
StyD4I	CCNGG	2 cut(s)	20, 495
StyI	CCWWGG	3 cut(s)	133, 182, 702
TaaI	ACNGT	4 cut(s)	40, 244, 744, 1454
TaiI	ACGT	3 cut(s)	668, 1091, 1097
TaqI	TCGA	7 cut(s)	407, 441, 530, 575, 1470, 1978, 2147
TasI	AATT	4 cut(s)	53, 290, 981, 2135
TatI	WGTACW	3 cut(s)	1307, 1344, 1995
TauI	GCSGC	3 cut(s)	8, 264, 389
TfiI	GAWTC	5 cut(s)	376, 865, 1190, 1538, 1685
TscAI	CASTG	2 cut(s)	1457, 1777
TseFI	GTSAC	1 cut(s)	738
TseI	GCWGC	3 cut(s)	1393, 1655, 1804
Tsp45I	GTSAC	1 cut(s)	738
TspDTI	ATGAA	8 cut(s)	334, 539, 639, 710, 710, 993, 1395, 2016
TspRI	CASTG	2 cut(s)	1457, 1777
Tth111I	GACNNNGTC	1 cut(s)	2096
Van91I	CCANNNNNTGG	1 cut(s)	183
Vha464I	CTTAAG	1 cut(s)	584
VpaK11BI	GGWCC	4 cut(s)	105, 420, 445, 1180
XapI	RAATTY	2 cut(s)	290, 2135
XbaI	TCTAGA	1 cut(s)	1594
XceI	RCATGY	2 cut(s)	1105, 1884
XhoI	CTCGAG	1 cut(s)	440
XmnI	GAANNNNTTC	1 cut(s)	1154
XspI	CTAG	7 cut(s)	390, 600, 957, 1028, 1490, 1595, 2063
ZrmI	AGTACT	1 cut(s)	1346
Zsp2I	ATGCAT	2 cut(s)	1113, 1674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.