MD02G1237400.v1.1

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004489
Physical Location & Seq
Reverse (-)
28489436 .. 28496386
6951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1237400.v1.1.491

Sequence Viewer

Length: 2604 bp
ATGACGGATTTTGAGCCGTTACAACAGAAACCAGAATCCGCTGATGCCTGTGCCGATTTTGAGCGTGGATTTGAGGAATTCATGCGGGGGCACTTGGATGAATGTATGTCATTTACATCATGTAGTTCTCCTCGTAATCCTGATGATGAAGATGACGAGGGTGAGCAGCTTGTGCGGAGGAGACGCAGGTTGGATTTGGAGGGCGATGATTTAGCAGAGTCATCTGCTGCGCGGTGCCACCACTCGCGGATTTTGAGCCGTTGGGCTGCGCGGCAGGCACAGGAGATGATAACAACGATTGAGAGGAGGAATCGTGAATCAGAGTTGATGGCACTTGCAGGGCTGCACACGGTTTCAATGCTTGACTCCTCATTCTTGAGGGAGTCGCAGTCTCCTACCTCAAGGCGGCAGGGGGCTGTGGAGAGGCCAAGCACTCAGGCATCTGCAATATTGCAAATGTGGAGGGAGTTAGAGGATGAGCATGTGTTGGATAGAGCGCGTGAAAGAGTGAGGGAAAGGTTGAGGCATAGGAGGAGTGTTGAGGCTAATACTAATGAGTCCACCACAAATATGTCTGAAAGTCGGGGGACTGAGAATCAGGGTAGTTTGGCGGATGCAAGTGAGAGTGAGAATGAGTTTGGAACTTGGTCACATGATCAAATAGGTTCACAACGTGAACATGGTGCTAATGACGTTTCTAGTAGAGAGCAATCTCCTGATCTTGGCGAAGTCGAGAGGGAGAGAGTGAGGCAGATTGTACGGGGATGGATGGAAACTGGGATTAGTGATCATTCGTCCAATGTTGCTCAGAGAAACACTAGTCCCAGATCAGAGTGGCTTGGTGAGACAGAGCGTGAAAGGGTGAGAATTGTACGTGAGTGGGTACAAATGGCAAGTCAGCAAAGAGGAGCTCGTGGGGGCCACAGGGATGATCAAGTTGCCAGTGGCGGTGCTCAAATTGATCGAGCTCATGATGGGACGGTTACTGACCATGAAGAAGGCCAAGCAGACCACATTCGCAGGGACATGCGGAGATTGCGTGGAAGACAAGCTCTAATTGATTTGCTTGTAAGGATTGAGAGGGAACGACAAAGGGAGCTCGAGTGTTTGGTGGAGCATCGGGCAGTCTCTGATTTTGCTCATCGTAACCGCATTCAGTCATTACTCAGAGGTAGATTCCTGCGAAATGAAAGACCTGTTGAAGAAGAAAGACCGCCTTCCATGGCTGCTGGTGAATTAGTTCAGTTGAGGCAGAGACACACAGTTTCTGGTTTAAGGGAAGGGTTCCGCTCCACGTTAGAAAATATTGTTCGTGGTCAAGTTGGCAGCCAAACTGATTCTACAACTAATGGTAATATTAATGATTTTAGAAGCGAGTATACTCAAACAAGTGCTTCACAGGATGTCCAACAGGAAAATAATGATCAATTGCAGCCTGGCAGTCAGGCAACTGACATCAATCAGTTGCCAGATCAAACGGGTAGCTTGGAGAGCAACACTACTGATGAGCTTCTAGATTGGCAAGAAACTACTAATCAAGGAGGGAATTGGCAGGAACCAGTGGCCGAAAATGACACACGAAACTGGCAGCAGCCAACCTTTGGTCAATTGAATGAATGGAGAGATGGTAATTCCGAAGATACGGCTGAAAATTGGCAAGGAAATTCAGTCGATAATTGGCCTGAGGAAACAACGAGAAACGTTGATGGAGAAGCAGATCATCGACAAGAAGCCCGGAGAATTTGGCATGAGAATGGTACTCGGGAGGCTGTTGGAAATTGGGCCGCAGGGCCTTCTGGTCCAGTGAGAAATCGCCGCTCCGTTCCAATTAGAAGATTCAATAGATTTCATCCGCCAGATGATGATAATGTGTACAGCATGGAACTCAGGGAACTTCTGAGCAGGAGAAGTGTCTCTAATCTTCTTCGCAGTGGGTTCCGTGAAAGTCTGGACCAACTGATTCAGTCATATGTTGAAAGGCGAAGTCATGCTCCCATTGATTGGGAATTGCATAGAAACTTGCCAACACCAACTCCAGCTTCACCAGAGCATGATCAGGAGCAACAGCGGGATGAGCAGAATGAAGATCAACATGATGCCATCAACAGACCTTCACTTGTTCTACCATCTCCACCAGTGCCCCCACCACAGCCATTATGGCATCAGGACTTACATCACACAGGCTGGTCTCGTCATAGCATGCATCGTTCAGAAATTGAATGGGAGATGATTAATGATCTCAGAGCAGACATGGCAAGACTTCAGCAAGGCATGAGTCACATGCAGAGGATGTTGGAGGCCTGCATGGATATGCAATTGGAGTTGCAACGATCTGTCAGACAGGAAGTCTCTGCAGCTTTGAATCGTTCATCTGGTGAAAAAGGTTTGGGCGCTGAGACATCAGAGGATGGGTCCAAATGGGGTCATGTGAGAAAAGGGACCTGTTGTGTGTGTTGTGATAGTCAAATCGATTCTCTCTTGTACAGATGCGGGCACATGTGCACTTGTTCAAAATGTGCGAACGAGTTGATTCGTGGCGGAGGGAAGTGCCCACTTTGTCGGGCACCGATTGTTGAGGTGATTCGAGCATACTCCATACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

868

Amino Acids

99.36

Weight (kDa)

5.38

Isoelectric Point (pI)

64.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 812 - 859 1.6e-11 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 177
AccB1I GGYRCC 2 cut(s) 234, 2563
AccB7I CCANNNNNTGG 2 cut(s) 1601, 2001
AccBSI CCGCTC 2 cut(s) 1290, 1818
AccI GTMKAC 1 cut(s) 1379
AccII CGCG 4 cut(s) 232, 247, 271, 499
AclI AACGTT 1 cut(s) 1701
AcoI YGGCCR 1 cut(s) 1563
AcsI RAATTY 3 cut(s) 77, 1663, 1740
AcuI CTGAAG 1 cut(s) 2246
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 6 cut(s) 759, 873, 885, 1759, 1874, 2483
AfiI CCNNNNNNNGG 4 cut(s) 405, 722, 1601, 2001
AflIII ACRYGT 1 cut(s) 2496
AgsI TTSAA 8 cut(s) 357, 1202, 1612, 1840, 1976, 2219, 2362, 2511
AhdI GACNNNNNGTC 1 cut(s) 2345
AhlI ACTAGT 1 cut(s) 818
AjnI CCWGG 1 cut(s) 1435
AjuI GAANNNNNNNTTGG 2 cut(s) 2023, 2055
AluBI AGCT 9 cut(s) 169, 911, 968, 1052, 1099, 1485, 1510, 2039, 2357
AluI AGCT 9 cut(s) 169, 911, 968, 1052, 1099, 1485, 1510, 2039, 2357
Alw21I GWGCWC 5 cut(s) 913, 955, 970, 1101, 2504
Alw26I GTCTC 9 cut(s) 175, 396, 839, 1132, 1249, 1918, 2193, 2353, 2390
Alw44I GTGCAC 1 cut(s) 2500
AlwNI CAGNNNCTG 2 cut(s) 1130, 1268
Ama87I CYCGRG 2 cut(s) 1100, 1761
AoxI GGCC 8 cut(s) 425, 919, 1000, 1563, 1679, 1782, 1790, 2298
ApaLI GTGCAC 1 cut(s) 2500
ApoI RAATTY 3 cut(s) 77, 1663, 1740
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AseI ATTAAT 2 cut(s) 1359, 2232
Asp700I GAANNNNTTC 1 cut(s) 1239
AspLEI GCGC 4 cut(s) 232, 271, 499, 2393
AspS9I GGNCC 7 cut(s) 919, 1782, 1790, 1799, 1951, 2412, 2439
AsuC2I CCSGG 1 cut(s) 1735
AsuHPI GGTGA 7 cut(s) 173, 854, 874, 1244, 2034, 2387, 2590
AvaI CYCGRG 2 cut(s) 1100, 1761
AvaII GGWCC 4 cut(s) 1799, 1951, 2412, 2439
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 6 cut(s) 93, 2142, 2496, 2504, 2552, 2566
BanI GGYRCC 2 cut(s) 234, 2563
BanII GRGCYC 3 cut(s) 913, 970, 1101
BarI GAAGNNNNNNTAC 2 cut(s) 1363, 1395
BauI CACGAG 1 cut(s) 912
BbsI GAAGAC 1 cut(s) 1051
Bbv12I GWGCWC 5 cut(s) 913, 955, 970, 1101, 2504
BccI CCATC 9 cut(s) 322, 759, 763, 968, 1619, 1700, 2108, 2134, 2402
BceAI ACGGC 2 cut(s) 243, 1659
BciT130I CCWGG 1 cut(s) 1437
BclI TGATCA 5 cut(s) 655, 787, 931, 1423, 2053
BcnI CCSGG 1 cut(s) 1735
BcoDI GTCTC 9 cut(s) 175, 396, 839, 1132, 1249, 1918, 2193, 2353, 2390
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 3 cut(s) 699, 819, 1514
BfmI CTRYAG 1 cut(s) 2352
BfoI RGCGCY 1 cut(s) 2394
BfuAI ACCTGC 1 cut(s) 177
BglI GCCNNNNNGGC 1 cut(s) 2158
Bme1390I CCNGG 2 cut(s) 1437, 1735
Bme18I GGWCC 4 cut(s) 1799, 1951, 2412, 2439
BmeRI GACNNNNNGTC 1 cut(s) 2345
BmeT110I CYCGRG 2 cut(s) 1100, 1761
BmgT120I GGNCC 7 cut(s) 919, 1782, 1790, 1799, 1951, 2412, 2439
BmiI GGNNCC 8 cut(s) 236, 920, 1286, 1557, 1937, 2413, 2440, 2565
BmrFI CCNGG 2 cut(s) 1437, 1735
BmrI ACTGGG 1 cut(s) 786
BmsI GCATC 8 cut(s) 34, 449, 604, 1126, 2086, 2170, 2212, 2477
BmuI ACTGGG 1 cut(s) 786
BpiI GAAGAC 1 cut(s) 1051
BpmI CTGGAG 1 cut(s) 2019
BpuEI CTTGAG 2 cut(s) 385, 397
BpuMI CCSGG 1 cut(s) 1735
Bsa29I ATCGAT 1 cut(s) 2469
BsaAI YACGTR 1 cut(s) 875
BsaI GGTCTC 1 cut(s) 2193
BsaJI CCNNGG 1 cut(s) 1221
Bsc4I CCNNNNNNNGG 4 cut(s) 405, 722, 1601, 2001
Bse1I ACTGG 6 cut(s) 781, 942, 1559, 1589, 1802, 2135
Bse21I CCTNAGG 1 cut(s) 1683
BseBI CCWGG 1 cut(s) 1437
BseCI ATCGAT 1 cut(s) 2469
BseDI CCNNGG 1 cut(s) 1221
BseLI CCNNNNNNNGG 4 cut(s) 405, 722, 1601, 2001
BseMII CTCAG 9 cut(s) 449, 582, 821, 1180, 1674, 1889, 1900, 2254, 2385
BseNI ACTGG 6 cut(s) 781, 942, 1559, 1589, 1802, 2135
BseRI GAGGAG 6 cut(s) 120, 193, 319, 358, 547, 921
BseSI GKGCMC 6 cut(s) 93, 2142, 2496, 2504, 2552, 2566
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 4 cut(s) 232, 247, 271, 499
BshFI GGCC 8 cut(s) 427, 921, 1002, 1565, 1681, 1784, 1792, 2300
BshNI GGYRCC 2 cut(s) 234, 2563
BshVI ATCGAT 1 cut(s) 2469
BsiHKAI GWGCWC 5 cut(s) 913, 955, 970, 1101, 2504
BsiHKCI CYCGRG 2 cut(s) 1100, 1761
BsiSI CCGG 1 cut(s) 1735
BslFI GGGAC 5 cut(s) 601, 807, 991, 1037, 2452
BslI CCNNNNNNNGG 4 cut(s) 405, 722, 1601, 2001
BsmAI GTCTC 9 cut(s) 175, 396, 839, 1132, 1249, 1918, 2193, 2353, 2390
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 5 cut(s) 601, 807, 991, 1037, 2452
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 8 cut(s) 427, 921, 1002, 1565, 1681, 1784, 1792, 2300
Bso31I GGTCTC 1 cut(s) 2193
BsoBI CYCGRG 2 cut(s) 1100, 1761
Bsp1407I TGTACA 2 cut(s) 1872, 2481
Bsp19I CCATGG 1 cut(s) 1221
BspANI GGCC 8 cut(s) 427, 921, 1002, 1565, 1681, 1784, 1792, 2300
BspCNI CTCAG 9 cut(s) 448, 583, 820, 1179, 1675, 1890, 1899, 2253, 2386
BspDI ATCGAT 1 cut(s) 2469
BspFNI CGCG 4 cut(s) 232, 247, 271, 499
BspHI TCATGA 1 cut(s) 970
BspLI GGNNCC 8 cut(s) 236, 920, 1286, 1557, 1937, 2413, 2440, 2565
BspMAI CTGCAG 1 cut(s) 2356
BspMI ACCTGC 1 cut(s) 177
BspT107I GGYRCC 2 cut(s) 234, 2563
BspTNI GGTCTC 1 cut(s) 2193
BsrBI CCGCTC 2 cut(s) 1290, 1818
BsrGI TGTACA 2 cut(s) 1872, 2481
BsrI ACTGG 6 cut(s) 781, 942, 1559, 1589, 1802, 2135
BssECI CCNNGG 1 cut(s) 1221
BssNAI GTATAC 1 cut(s) 1380
BssSI CACGAG 1 cut(s) 912
BssT1I CCWWGG 1 cut(s) 1221
Bst1107I GTATAC 1 cut(s) 1380
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 1 cut(s) 1437
Bst4CI ACNGT 4 cut(s) 352, 982, 1264, 2601
BstAPI GCANNNNNTGC 1 cut(s) 172
BstAUI TGTACA 2 cut(s) 1872, 2481
BstBAI YACGTR 1 cut(s) 875
BstC8I GCNNGC 4 cut(s) 276, 2201, 2302, 2492
BstDEI CTNAG 9 cut(s) 435, 591, 807, 1166, 1683, 1886, 1898, 2240, 2394
BstDSI CCRYGG 1 cut(s) 1221
BstFNI CGCG 4 cut(s) 232, 247, 271, 499
BstH2I RGCGCY 1 cut(s) 2394
BstHHI GCGC 4 cut(s) 232, 271, 499, 2393
BstMAI GTCTC 9 cut(s) 175, 396, 839, 1132, 1249, 1918, 2193, 2353, 2390
BstMWI GCNNNNNNNGC 7 cut(s) 172, 275, 1036, 1491, 2074, 2158, 2252
BstNI CCWGG 1 cut(s) 1437
BstNSI RCATGY 5 cut(s) 485, 1030, 2203, 2284, 2500
BstSCI CCNGG 2 cut(s) 1435, 1733
BstSFI CTRYAG 1 cut(s) 2352
BstSLI GKGCMC 6 cut(s) 93, 2142, 2496, 2504, 2552, 2566
BstUI CGCG 4 cut(s) 232, 247, 271, 499
BstV2I GAAGAC 1 cut(s) 1051
BstZ17I GTATAC 1 cut(s) 1380
Bsu15I ATCGAT 1 cut(s) 2469
Bsu36I CCTNAGG 1 cut(s) 1683
BsuRI GGCC 8 cut(s) 427, 921, 1002, 1565, 1681, 1784, 1792, 2300
BsuTUI ATCGAT 1 cut(s) 2469
BtgI CCRYGG 1 cut(s) 1221
BtgZI GCGATG 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 1936
BtsIMutI CAGTG 5 cut(s) 949, 1566, 1809, 1936, 2142
BveI ACCTGC 1 cut(s) 177
Cac8I GCNNGC 4 cut(s) 276, 2201, 2302, 2492
CaiI CAGNNNCTG 2 cut(s) 1130, 1268
CciI TCATGA 1 cut(s) 970
CfoI GCGC 4 cut(s) 232, 271, 499, 2393
Cfr13I GGNCC 7 cut(s) 919, 1782, 1790, 1799, 1951, 2412, 2439
ClaI ATCGAT 1 cut(s) 2469
CseI GACGC 1 cut(s) 192
Csp6I GTAC 6 cut(s) 758, 872, 884, 1758, 1873, 2482
CviQI GTAC 6 cut(s) 758, 872, 884, 1758, 1873, 2482
DdeI CTNAG 9 cut(s) 435, 591, 807, 1166, 1683, 1886, 1898, 2240, 2394
DraIII CACNNNGTG 1 cut(s) 674
DriI GACNNNNNGTC 1 cut(s) 2345
EaeI YGGCCR 1 cut(s) 1563
Eam1105I GACNNNNNGTC 1 cut(s) 2345
EciI GGCGGA 3 cut(s) 626, 1842, 2553
Ecl136II GAGCTC 3 cut(s) 911, 968, 1099
Eco130I CCWWGG 1 cut(s) 1221
Eco147I AGGCCT 1 cut(s) 2300
Eco24I GRGCYC 3 cut(s) 913, 970, 1101
Eco31I GGTCTC 1 cut(s) 2193
Eco47I GGWCC 4 cut(s) 1799, 1951, 2412, 2439
Eco53kI GAGCTC 3 cut(s) 911, 968, 1099
Eco57I CTGAAG 1 cut(s) 2246
Eco81I CCTNAGG 1 cut(s) 1683
Eco88I CYCGRG 2 cut(s) 1100, 1761
EcoICRI GAGCTC 3 cut(s) 911, 968, 1099
EcoO109I RGGNCCY 2 cut(s) 1790, 2439
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 1435
EcoT14I CCWWGG 1 cut(s) 1221
EcoT22I ATGCAT 1 cut(s) 2205
EcoT38I GRGCYC 3 cut(s) 913, 970, 1101
ErhI CCWWGG 1 cut(s) 1221
Esp3I CGTCTC 1 cut(s) 175
FalI AAGNNNNNCTT 2 cut(s) 1201, 1233
FaqI GGGAC 5 cut(s) 601, 807, 991, 1037, 2452
FauI CCCGC 3 cut(s) 78, 2061, 2483
FauNDI CATATG 1 cut(s) 1969
FbaI TGATCA 5 cut(s) 655, 787, 931, 1423, 2053
FblI GTMKAC 1 cut(s) 1379
FriOI GRGCYC 3 cut(s) 913, 970, 1101
FspBI CTAG 3 cut(s) 699, 819, 1514
GlaI GCGC 4 cut(s) 231, 270, 498, 2392
GsuI CTGGAG 1 cut(s) 2019
HaeII RGCGCY 1 cut(s) 2394
HaeIII GGCC 8 cut(s) 427, 921, 1002, 1565, 1681, 1784, 1792, 2300
HapII CCGG 1 cut(s) 1735
HgaI GACGC 1 cut(s) 192
HhaI GCGC 4 cut(s) 232, 271, 499, 2393
Hin6I GCGC 4 cut(s) 230, 269, 497, 2391
HinP1I GCGC 4 cut(s) 230, 269, 497, 2391
HpaII CCGG 1 cut(s) 1735
HphI GGTGA 7 cut(s) 173, 854, 874, 1244, 2034, 2387, 2590
Hpy166II GTNNAC 6 cut(s) 561, 668, 677, 1380, 1873, 2502
Hpy8I GTNNAC 6 cut(s) 561, 668, 677, 1380, 1873, 2502
HpyAV CCTTC 5 cut(s) 992, 1227, 1274, 1803, 2121
HpyCH4III ACNGT 4 cut(s) 352, 982, 1264, 2601
HpyCH4IV ACGT 5 cut(s) 673, 693, 874, 1295, 1701
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 275, 1036, 1491, 2074, 2158, 2252
HpyF3I CTNAG 9 cut(s) 435, 591, 807, 1166, 1683, 1886, 1898, 2240, 2394
HpySE526I ACGT 5 cut(s) 673, 693, 874, 1295, 1701
HspAI GCGC 4 cut(s) 230, 269, 497, 2391
Ksp22I TGATCA 5 cut(s) 655, 787, 931, 1423, 2053
LmnI GCTCC 7 cut(s) 908, 1096, 1114, 1295, 1823, 1996, 2059
LweI GCATC 8 cut(s) 34, 449, 604, 1126, 2086, 2170, 2212, 2477
MaeI CTAG 3 cut(s) 699, 819, 1514
MaeII ACGT 5 cut(s) 673, 693, 874, 1295, 1701
MaeIII GTNAC 5 cut(s) 18, 648, 982, 1145, 2276
MbiI CCGCTC 2 cut(s) 1290, 1818
MfeI CAATTG 3 cut(s) 1427, 1607, 2315
MlyI GAGTC 5 cut(s) 227, 359, 392, 566, 2284
MmeI TCCRAC 5 cut(s) 171, 468, 1432, 1753, 2274
Mph1103I ATGCAT 1 cut(s) 2205
MroXI GAANNNNTTC 1 cut(s) 1239
MseI TTAA 3 cut(s) 1274, 1359, 2232
MslI CAYNNNNRTG 5 cut(s) 96, 569, 927, 1752, 2309
MspA1I CMGCKG 2 cut(s) 41, 2068
MspI CCGG 1 cut(s) 1735
MspR9I CCNGG 2 cut(s) 1437, 1735
MunI CAATTG 3 cut(s) 1427, 1607, 2315
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 1 cut(s) 1437
MvnI CGCG 4 cut(s) 232, 247, 271, 499
MwoI GCNNNNNNNGC 7 cut(s) 172, 275, 1036, 1491, 2074, 2158, 2252
NciI CCSGG 1 cut(s) 1735
NcoI CCATGG 1 cut(s) 1221
NdeI CATATG 1 cut(s) 1969
NlaIV GGNNCC 8 cut(s) 236, 920, 1286, 1557, 1937, 2413, 2440, 2565
NmuCI GTSAC 2 cut(s) 648, 2276
NsiI ATGCAT 1 cut(s) 2205
NspI RCATGY 5 cut(s) 485, 1030, 2203, 2284, 2500
PaeI GCATGC 1 cut(s) 2203
PaeR7I CTCGAG 1 cut(s) 1100
PagI TCATGA 1 cut(s) 970
PceI AGGCCT 1 cut(s) 2300
PciI ACATGT 1 cut(s) 2496
PctI GAATGC 1 cut(s) 1152
PdmI GAANNNNTTC 1 cut(s) 1239
PflMI CCANNNNNTGG 2 cut(s) 1601, 2001
PleI GAGTC 5 cut(s) 226, 359, 391, 565, 2283
PpsI GAGTC 5 cut(s) 226, 359, 391, 565, 2283
Ppu21I YACGTR 1 cut(s) 875
PpuMI RGGWCCY 1 cut(s) 2439
PscI ACATGT 1 cut(s) 2496
PshBI ATTAAT 2 cut(s) 1359, 2232
Psp124BI GAGCTC 3 cut(s) 913, 970, 1101
Psp1406I AACGTT 1 cut(s) 1701
Psp5II RGGWCCY 1 cut(s) 2439
Psp6I CCWGG 1 cut(s) 1435
PspGI CCWGG 1 cut(s) 1435
PspN4I GGNNCC 8 cut(s) 236, 920, 1286, 1557, 1937, 2413, 2440, 2565
PspPI GGNCC 7 cut(s) 919, 1782, 1790, 1799, 1951, 2412, 2439
PspPPI RGGWCCY 1 cut(s) 2439
PspXI VCTCGAGB 1 cut(s) 1100
PstI CTGCAG 1 cut(s) 2356
PstNI CAGNNNCTG 2 cut(s) 1130, 1268
RsaI GTAC 6 cut(s) 759, 873, 885, 1759, 1874, 2483
RsaNI GTAC 6 cut(s) 758, 872, 884, 1758, 1873, 2482
RseI CAYNNNNRTG 5 cut(s) 96, 569, 927, 1752, 2309
SacI GAGCTC 3 cut(s) 913, 970, 1101
SaqAI TTAA 3 cut(s) 1274, 1359, 2232
Sau96I GGNCC 7 cut(s) 919, 1782, 1790, 1799, 1951, 2412, 2439
SchI GAGTC 5 cut(s) 227, 359, 392, 566, 2284
ScrFI CCNGG 2 cut(s) 1437, 1735
SfaNI GCATC 8 cut(s) 34, 449, 604, 1126, 2086, 2170, 2212, 2477
SfcI CTRYAG 1 cut(s) 2352
Sfr274I CTCGAG 1 cut(s) 1100
SinI GGWCC 4 cut(s) 1799, 1951, 2412, 2439
SlaI CTCGAG 1 cut(s) 1100
SmiMI CAYNNNNRTG 5 cut(s) 96, 569, 927, 1752, 2309
SmlI CTYRAG 3 cut(s) 376, 400, 1100
SmoI CTYRAG 3 cut(s) 376, 400, 1100
SpeI ACTAGT 1 cut(s) 818
SphI GCATGC 1 cut(s) 2203
SseBI AGGCCT 1 cut(s) 2300
SspI AATATT 3 cut(s) 450, 1306, 1357
SspMI CTAG 3 cut(s) 699, 819, 1514
SstI GAGCTC 3 cut(s) 913, 970, 1101
StuI AGGCCT 1 cut(s) 2300
StyD4I CCNGG 2 cut(s) 1435, 1733
StyI CCWWGG 1 cut(s) 1221
TaaI ACNGT 4 cut(s) 352, 982, 1264, 2601
TaiI ACGT 5 cut(s) 676, 696, 877, 1298, 1704
TaqI TCGA 7 cut(s) 732, 964, 1101, 1671, 1723, 2469, 2584
TatI WGTACW 2 cut(s) 1872, 2481
TauI GCSGC 4 cut(s) 274, 409, 1787, 1818
Tru1I TTAA 3 cut(s) 1274, 1359, 2232
Tru9I TTAA 3 cut(s) 1274, 1359, 2232
TscAI CASTG 5 cut(s) 949, 1566, 1809, 1936, 2142
TseFI GTSAC 2 cut(s) 648, 2276
Tsp45I GTSAC 2 cut(s) 648, 2276
TspDTI ATGAA 9 cut(s) 70, 114, 162, 1008, 1203, 1629, 1838, 2097, 2358
TspGWI ACGGA 3 cut(s) 20, 1810, 1928
TspRI CASTG 5 cut(s) 949, 1566, 1809, 1936, 2142
Van91I CCANNNNNTGG 2 cut(s) 1601, 2001
VneI GTGCAC 1 cut(s) 2500
VpaK11BI GGWCC 4 cut(s) 1799, 1951, 2412, 2439
VspI ATTAAT 2 cut(s) 1359, 2232
XapI RAATTY 3 cut(s) 77, 1663, 1740
XbaI TCTAGA 1 cut(s) 1513
XceI RCATGY 5 cut(s) 485, 1030, 2203, 2284, 2500
XcmI CCANNNNNNNNNTGG 1 cut(s) 2154
XhoI CTCGAG 1 cut(s) 1100
XmiI GTMKAC 1 cut(s) 1379
XmnI GAANNNNTTC 1 cut(s) 1239
XspI CTAG 3 cut(s) 699, 819, 1514
Zsp2I ATGCAT 1 cut(s) 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.