MD07G1074600.v1.1

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004489
Physical Location & Seq
Forward (+)
7121816 .. 7128140
6325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1074600.v1.1.491

Sequence Viewer

Length: 2604 bp
ATGACGGATTTTGAGCCGTTACAACAGAAGCCGGAATCTGCTGATGCCTGTGCCGATTTTGAGCGTGGATTTGAGGAATTCATGCGGGGGCACTTGGATGAATGTATGTCATTTGCATCATGTAGTTCTCCTCGTAATCCTGATGATGAAGATGACGAGGGTGAGCAGCTTGTGCGGAGGAGACGGAGGTTGGATCTGGAGGGTGATGATTTAGCAGAGTCATCTGCTGCACGGCGACACCACTCGCGGATTCTGAGCCGTTGGGCTGCGCAGCAGGCGCAGGAAATGATAACAACGATTGAGAGGAGGAATCGTGAATCAGAGTTGATGGCACTTGCAGGCCTGCACACGGTTTCGATGCTTGACTCCTCGTTCTTGAGGGAGTCACAGTCTCCTACCTCAAGGCGGCAGGGGGCTGTTGAGAGGCCAAGTACTCAGGCATCAGCAATATTGCAAATGTGGAGGGAATTAGAGGATGAGCATGTGTTGAATAGAGCGCGTGAAAGAGTGAGGGAAAGGTTGAGGCATAGGAGGAGTGTTGAGTCTAATACTAATGACTCGACCACAAATATGTCTGATAGTCGGGGGAGTGAGAATCAGGGTAGTTTGGCGGATGCAAGTGAGAGTGAGAATGAGTTTGGAACTTGGTCACATGATCAAATAGGTTCACAACGTGAACACGGTGCCAATGACGTGTCTAGTAGAGAGCAATCTCCTGATCTTGGTGAAGTGGAGAGGGAGAGAGTGAGTCAGATTGTACGGGGATGGATGGAAACTGGCATTGGTGATCGTTCATCCAATGTTGCTCAGAGAAACACTAGTCCCAGAGCAGAGTGGCTTGGTGAGACAGAGCGTGAAAGGGTGAGAATTGTTCGTGAGTGGGTGCAAATGGCAAGTCAGCAAAGAGGAGCTCGTGGGGGCCAGAGGGATGATCAAGTTGCCAGTGGTGGTGCTCAAGTTGATCGAGCTCGTGACGGGACGGTTGCTGACCATGAAGAAGGCCAACCAGACCACATTCGCAGGGACATGCGGAGATTTCGTGGAAGACAAGCTCTAATTGATTTGCTTGTAAGAATTGAGAGGGAACGACAAAGGGAGCTCGATTGTTTGGTGGAGCATCGGGCTGTCTCTGATTTTGCTCATCGAAACCGCATTCAGTCATTACTCAGAGGTAGATTCCTGCGCAATGAAAGACCTGTTGAAGAAGAAAGACCACCTTCCATGGCTGCTGGTGAATTAGTCCAGTTGAGACAGCGACACACTGTTTCTGGTTTAAGGGAAGGGTTCCGCTACAGATTAGAAAATATTGTTCGTGGTCAGGTTGGCAGCCAAACCGATTCTACGATTAACAGTAATATTAATGAATCTAGAAGTGAGCATACTCAAACAAATGCTTCACAGGATGTCCAACAGGAAAGTAATGATCAATTGCAGCCTGGTAGTCAGGCAACTGACATCAATCAGTTGCCTGATCAAATGGGAAGCTTGGAGAGCAACACTACTGATGAGCGTCAAGATTGGCAAGAAACTGCTAATCAAGGAGGGAATTGGCAGGAACCAGTTGTTGAAGATGAGACACGAAACTTGGAGCAGCCAACTTTCGGTCAGTTGAATGAATGGAGAAATGGTAATGCCGAAGATACGGCTGAAAATTGGCCAGGAAATTCAGTCAATAATTGGCCTGAGGAAACACCCAGAAATGTTGATGGAGAAGCAGATCATCGACAAGAAGCCCAGGGAATTTGGCATGAGAATGGTACTCGGGATGCTGTTGGAAATTGGACCGAAGGGCCTTCTGGTCCCATGAGAAATCGCCGTTCAGTTCCAATTAGAAGATTCAATAGATTTCATCCGCCAGAGGATGATAATGTGTACAGCATGGAACTCAGGGAACTTCTGAGCAGGAGAAGTGTCTCTAATCTTCTTCGGAGTGGGTTCCGTGAAAGTCTGGACCAACTGATTCAGTCATATGTTGAAAGGCAAAGTCATGCTCCCATTGATTGGGATTTGCATAGAAACTTGCCAACACCAATTCCAGCTTCACCAGAGCATGATCAGGAGCAACAGCGGGATGGGCAGAATGAAGATCAACACGATGCCATTAACAGACCTTCACTTGTTCTACCATCTCCACCAGTGCCCCCACCACAGCCATTATGGCACCAGGACCTACATCACACAGGCTGGTCTCGTCATAGCATGCATCGTTCAGAAATTGAATGGGAGATGATTAATGATCTCAGAGCAGACATGGGTAGACTCCAGCAAGGCATGAGTCACATGCAGAGGATGTTGGAGGCCTGCATGGATATGCAATTGGAGTTGCAACGTTCTGTTAGACAGGAAGTCTCCGCAGCTTTGAATCGTTCATCTGGTGAAAAAGGCTTGGGCGCTGAGACATCAGAGGATGGGTCCAAATGGGGTCATGTGAGAAAAGGGACCTGTTGTGTTTGTTGTGATAGTCAAATCGATTCTCTCTTGTACAGATGCGGGCACATGTGCACTTGTTCAAAGTGTGCAAATGAGTTGATTCGTGGCGGAGGGAAGTGCCCACTTTGTCGAGCACCGATTGTTGAGGTGATTCGAGCATACTCCATACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

868

Amino Acids

99.19

Weight (kDa)

5.36

Isoelectric Point (pI)

63.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 812 - 859 1.6e-11 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 270, 1184
AccB1I GGYRCC 2 cut(s) 683, 2160
AccB7I CCANNNNNTGG 1 cut(s) 2001
AccI GTMKAC 1 cut(s) 2257
AccII CGCG 2 cut(s) 247, 499
AclI AACGTT 1 cut(s) 2329
AclWI GGATC 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 1655
AcsI RAATTY 3 cut(s) 77, 1663, 1740
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 5 cut(s) 433, 759, 1759, 1874, 2483
AfiI CCNNNNNNNGG 5 cut(s) 349, 405, 722, 1601, 2001
AflIII ACRYGT 2 cut(s) 693, 2496
AgsI TTSAA 9 cut(s) 490, 1202, 1568, 1612, 1840, 1976, 2219, 2362, 2511
AhdI GACNNNNNGTC 1 cut(s) 2345
AhlI ACTAGT 1 cut(s) 818
AjiI CACGTC 1 cut(s) 694
AjnI CCWGG 4 cut(s) 1435, 1657, 1734, 2163
AjuI GAANNNNNNNTTGG 4 cut(s) 765, 797, 2023, 2055
AluBI AGCT 8 cut(s) 169, 911, 968, 1052, 1099, 1485, 2039, 2357
AluI AGCT 8 cut(s) 169, 911, 968, 1052, 1099, 1485, 2039, 2357
Alw21I GWGCWC 6 cut(s) 913, 955, 970, 1101, 2504, 2566
Alw44I GTGCAC 1 cut(s) 2500
AlwI GGATC 1 cut(s) 201
Ama87I CYCGRG 1 cut(s) 1761
AoxI GGCC 8 cut(s) 340, 425, 919, 1000, 1655, 1679, 1790, 2298
ApaLI GTGCAC 1 cut(s) 2500
ApeKI GCWGC 9 cut(s) 166, 227, 266, 271, 1226, 1326, 1432, 1591, 2354
ApoI RAATTY 3 cut(s) 77, 1663, 1740
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AseI ATTAAT 2 cut(s) 1359, 2232
AspLEI GCGC 5 cut(s) 271, 280, 499, 1185, 2393
AspS9I GGNCC 8 cut(s) 919, 1782, 1790, 1799, 1951, 2167, 2412, 2439
AvaI CYCGRG 1 cut(s) 1761
AvaII GGWCC 6 cut(s) 1782, 1799, 1951, 2167, 2412, 2439
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 5 cut(s) 93, 2142, 2496, 2504, 2552
BalI TGGCCA 1 cut(s) 1657
BanI GGYRCC 2 cut(s) 683, 2160
BanII GRGCYC 3 cut(s) 913, 970, 1101
BarI GAAGNNNNNNTAC 2 cut(s) 1363, 1395
BauI CACGAG 2 cut(s) 912, 969
BbsI GAAGAC 1 cut(s) 1051
Bbv12I GWGCWC 6 cut(s) 913, 955, 970, 1101, 2504, 2566
BbvI GCAGC 9 cut(s) 178, 214, 253, 283, 1213, 1338, 1444, 1603, 2366
BccI CCATC 7 cut(s) 322, 759, 763, 1700, 2066, 2134, 2402
BceAI ACGGC 4 cut(s) 243, 248, 1659, 1800
BciT130I CCWGG 4 cut(s) 1437, 1659, 1736, 2165
BclI TGATCA 5 cut(s) 655, 931, 1423, 1471, 2053
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 3 cut(s) 699, 819, 1368
BfmI CTRYAG 1 cut(s) 1291
BfoI RGCGCY 1 cut(s) 2394
BglI GCCNNNNNGGC 1 cut(s) 2158
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 4 cut(s) 1437, 1659, 1736, 2165
Bme18I GGWCC 6 cut(s) 1782, 1799, 1951, 2167, 2412, 2439
BmeRI GACNNNNNGTC 1 cut(s) 2345
BmeT110I CYCGRG 1 cut(s) 1761
BmgBI CACGTC 1 cut(s) 694
BmgT120I GGNCC 8 cut(s) 919, 1782, 1790, 1799, 1951, 2167, 2412, 2439
BmiI GGNNCC 9 cut(s) 685, 920, 1286, 1557, 1801, 1937, 2162, 2413, 2440
BmrFI CCNGG 4 cut(s) 1437, 1659, 1736, 2165
BpiI GAAGAC 1 cut(s) 1051
BpmI CTGGAG 2 cut(s) 218, 2246
BpuEI CTTGAG 3 cut(s) 385, 397, 939
Bsa29I ATCGAT 1 cut(s) 2469
BsaI GGTCTC 1 cut(s) 2193
BsaJI CCNNGG 3 cut(s) 1221, 1734, 1735
BsaXI ACNNNNNCTCC 8 cut(s) 298, 328, 374, 404, 1024, 1054, 1612, 1642
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 405, 722, 1601, 2001
Bse1I ACTGG 5 cut(s) 781, 942, 1243, 1559, 2135
Bse21I CCTNAGG 1 cut(s) 1683
Bse3DI GCAATG 1 cut(s) 1192
BseBI CCWGG 4 cut(s) 1437, 1659, 1736, 2165
BseCI ATCGAT 1 cut(s) 2469
BseDI CCNNGG 3 cut(s) 1221, 1734, 1735
BseLI CCNNNNNNNGG 5 cut(s) 349, 405, 722, 1601, 2001
BseMI GCAATG 1 cut(s) 1192
BseMII CTCAG 9 cut(s) 245, 449, 821, 1180, 1674, 1889, 1900, 2254, 2385
BseNI ACTGG 5 cut(s) 781, 942, 1243, 1559, 2135
BseRI GAGGAG 6 cut(s) 120, 193, 319, 358, 547, 921
BseSI GKGCMC 5 cut(s) 93, 2142, 2496, 2504, 2552
BseXI GCAGC 9 cut(s) 178, 214, 253, 283, 1213, 1338, 1444, 1603, 2366
BsgI GTGCAG 2 cut(s) 213, 329
Bsh1236I CGCG 2 cut(s) 247, 499
BshFI GGCC 8 cut(s) 342, 427, 921, 1002, 1657, 1681, 1792, 2300
BshNI GGYRCC 2 cut(s) 683, 2160
BshVI ATCGAT 1 cut(s) 2469
BsiHKAI GWGCWC 6 cut(s) 913, 955, 970, 1101, 2504, 2566
BsiHKCI CYCGRG 1 cut(s) 1761
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 5 cut(s) 807, 991, 1037, 1785, 2452
BslI CCNNNNNNNGG 5 cut(s) 349, 405, 722, 1601, 2001
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 5 cut(s) 807, 991, 1037, 1785, 2452
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 8 cut(s) 342, 427, 921, 1002, 1657, 1681, 1792, 2300
Bso31I GGTCTC 1 cut(s) 2193
BsoBI CYCGRG 1 cut(s) 1761
Bsp1407I TGTACA 2 cut(s) 1872, 2481
Bsp19I CCATGG 1 cut(s) 1221
BspANI GGCC 8 cut(s) 342, 427, 921, 1002, 1657, 1681, 1792, 2300
BspCNI CTCAG 9 cut(s) 246, 448, 820, 1179, 1675, 1890, 1899, 2253, 2386
BspDI ATCGAT 1 cut(s) 2469
BspFNI CGCG 2 cut(s) 247, 499
BspLI GGNNCC 9 cut(s) 685, 920, 1286, 1557, 1801, 1937, 2162, 2413, 2440
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 2 cut(s) 683, 2160
BspTNI GGTCTC 1 cut(s) 2193
BsrDI GCAATG 1 cut(s) 1192
BsrGI TGTACA 2 cut(s) 1872, 2481
BsrI ACTGG 5 cut(s) 781, 942, 1243, 1559, 2135
BssECI CCNNGG 3 cut(s) 1221, 1734, 1735
BssSI CACGAG 2 cut(s) 912, 969
BssT1I CCWWGG 1 cut(s) 1221
Bst2BI CACGAG 2 cut(s) 912, 969
Bst2UI CCWGG 4 cut(s) 1437, 1659, 1736, 2165
Bst4CI ACNGT 7 cut(s) 352, 390, 683, 982, 1264, 1352, 2601
BstAPI GCANNNNNTGC 1 cut(s) 172
BstAUI TGTACA 2 cut(s) 1872, 2481
BstC8I GCNNGC 6 cut(s) 276, 340, 344, 2201, 2302, 2492
BstDEI CTNAG 9 cut(s) 254, 435, 807, 1166, 1683, 1886, 1898, 2240, 2394
BstDSI CCRYGG 1 cut(s) 1221
BstFNI CGCG 2 cut(s) 247, 499
BstH2I RGCGCY 1 cut(s) 2394
BstHHI GCGC 5 cut(s) 271, 280, 499, 1185, 2393
BstMWI GCNNNNNNNGC 6 cut(s) 172, 275, 277, 1491, 2074, 2158
BstNI CCWGG 4 cut(s) 1437, 1659, 1736, 2165
BstNSI RCATGY 5 cut(s) 485, 1030, 2203, 2284, 2500
BstSCI CCNGG 4 cut(s) 1435, 1657, 1734, 2163
BstSFI CTRYAG 1 cut(s) 1291
BstSLI GKGCMC 5 cut(s) 93, 2142, 2496, 2504, 2552
BstUI CGCG 2 cut(s) 247, 499
BstV1I GCAGC 9 cut(s) 178, 214, 253, 283, 1213, 1338, 1444, 1603, 2366
BstV2I GAAGAC 1 cut(s) 1051
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 2469
Bsu36I CCTNAGG 1 cut(s) 1683
BsuRI GGCC 8 cut(s) 342, 427, 921, 1002, 1657, 1681, 1792, 2300
BsuTUI ATCGAT 1 cut(s) 2469
BtgI CCRYGG 1 cut(s) 1221
BtrI CACGTC 1 cut(s) 694
BtsIMutI CAGTG 3 cut(s) 949, 1260, 2142
Cac8I GCNNGC 6 cut(s) 276, 340, 344, 2201, 2302, 2492
CfoI GCGC 5 cut(s) 271, 280, 499, 1185, 2393
Cfr13I GGNCC 8 cut(s) 919, 1782, 1790, 1799, 1951, 2167, 2412, 2439
ClaI ATCGAT 1 cut(s) 2469
CseI GACGC 1 cut(s) 1499
Csp6I GTAC 5 cut(s) 432, 758, 1758, 1873, 2482
CviQI GTAC 5 cut(s) 432, 758, 1758, 1873, 2482
DdeI CTNAG 9 cut(s) 254, 435, 807, 1166, 1683, 1886, 1898, 2240, 2394
DraIII CACNNNGTG 1 cut(s) 674
DriI GACNNNNNGTC 1 cut(s) 2345
EaeI YGGCCR 1 cut(s) 1655
Eam1105I GACNNNNNGTC 1 cut(s) 2345
EciI GGCGGA 3 cut(s) 626, 1842, 2553
Ecl136II GAGCTC 3 cut(s) 911, 968, 1099
Eco130I CCWWGG 1 cut(s) 1221
Eco147I AGGCCT 2 cut(s) 342, 2300
Eco24I GRGCYC 3 cut(s) 913, 970, 1101
Eco31I GGTCTC 1 cut(s) 2193
Eco47I GGWCC 6 cut(s) 1782, 1799, 1951, 2167, 2412, 2439
Eco53kI GAGCTC 3 cut(s) 911, 968, 1099
Eco81I CCTNAGG 1 cut(s) 1683
Eco88I CYCGRG 1 cut(s) 1761
EcoICRI GAGCTC 3 cut(s) 911, 968, 1099
EcoO109I RGGNCCY 3 cut(s) 1790, 2167, 2439
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 4 cut(s) 1435, 1657, 1734, 2163
EcoT14I CCWWGG 1 cut(s) 1221
EcoT22I ATGCAT 1 cut(s) 2205
EcoT38I GRGCYC 3 cut(s) 913, 970, 1101
ErhI CCWWGG 1 cut(s) 1221
Esp3I CGTCTC 1 cut(s) 175
FalI AAGNNNNNCTT 2 cut(s) 1201, 1233
FaqI GGGAC 5 cut(s) 807, 991, 1037, 1785, 2452
FauI CCCGC 3 cut(s) 78, 2061, 2483
FauNDI CATATG 1 cut(s) 1969
FbaI TGATCA 5 cut(s) 655, 931, 1423, 1471, 2053
FblI GTMKAC 1 cut(s) 2257
FriOI GRGCYC 3 cut(s) 913, 970, 1101
FspBI CTAG 3 cut(s) 699, 819, 1368
FspI TGCGCA 2 cut(s) 270, 1184
GlaI GCGC 5 cut(s) 270, 279, 498, 1184, 2392
GsuI CTGGAG 2 cut(s) 218, 2246
HaeII RGCGCY 1 cut(s) 2394
HaeIII GGCC 8 cut(s) 342, 427, 921, 1002, 1657, 1681, 1792, 2300
HapII CCGG 1 cut(s) 32
HgaI GACGC 1 cut(s) 1499
HhaI GCGC 5 cut(s) 271, 280, 499, 1185, 2393
Hin6I GCGC 5 cut(s) 269, 278, 497, 1183, 2391
HinP1I GCGC 5 cut(s) 269, 278, 497, 1183, 2391
HindIII AAGCTT 1 cut(s) 1483
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 5 cut(s) 668, 677, 1873, 2258, 2502
Hpy8I GTNNAC 5 cut(s) 668, 677, 1873, 2258, 2502
HpyAV CCTTC 6 cut(s) 992, 1227, 1274, 1781, 1803, 2121
HpyCH4III ACNGT 7 cut(s) 352, 390, 683, 982, 1264, 1352, 2601
HpyCH4IV ACGT 3 cut(s) 673, 693, 2329
HpyF10VI GCNNNNNNNGC 6 cut(s) 172, 275, 277, 1491, 2074, 2158
HpyF3I CTNAG 9 cut(s) 254, 435, 807, 1166, 1683, 1886, 1898, 2240, 2394
HpySE526I ACGT 3 cut(s) 673, 693, 2329
HspAI GCGC 5 cut(s) 269, 278, 497, 1183, 2391
Ksp22I TGATCA 5 cut(s) 655, 931, 1423, 1471, 2053
LmnI GCTCC 6 cut(s) 908, 1096, 1114, 1588, 1996, 2059
Lsp1109I GCAGC 9 cut(s) 178, 214, 253, 283, 1213, 1338, 1444, 1603, 2366
MaeI CTAG 3 cut(s) 699, 819, 1368
MaeII ACGT 3 cut(s) 673, 693, 2329
MaeIII GTNAC 5 cut(s) 18, 384, 648, 971, 2276
MfeI CAATTG 2 cut(s) 1427, 2315
MflI RGATCY 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 1657
MluNI TGGCCA 1 cut(s) 1657
MlyI GAGTC 8 cut(s) 227, 359, 392, 551, 551, 757, 2253, 2284
MmeI TCCRAC 4 cut(s) 171, 1432, 1753, 2274
Mox20I TGGCCA 1 cut(s) 1657
Mph1103I ATGCAT 1 cut(s) 2205
MscI TGGCCA 1 cut(s) 1657
MseI TTAA 5 cut(s) 1274, 1347, 1359, 2103, 2232
MslI CAYNNNNRTG 4 cut(s) 96, 569, 1752, 2309
Msp20I TGGCCA 1 cut(s) 1657
MspA1I CMGCKG 1 cut(s) 2068
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 4 cut(s) 1437, 1659, 1736, 2165
MunI CAATTG 2 cut(s) 1427, 2315
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 4 cut(s) 1437, 1659, 1736, 2165
MvnI CGCG 2 cut(s) 247, 499
MwoI GCNNNNNNNGC 6 cut(s) 172, 275, 277, 1491, 2074, 2158
NcoI CCATGG 1 cut(s) 1221
NdeI CATATG 1 cut(s) 1969
NlaIV GGNNCC 9 cut(s) 685, 920, 1286, 1557, 1801, 1937, 2162, 2413, 2440
NmuCI GTSAC 4 cut(s) 384, 648, 971, 2276
NsbI TGCGCA 2 cut(s) 270, 1184
NsiI ATGCAT 1 cut(s) 2205
NspI RCATGY 5 cut(s) 485, 1030, 2203, 2284, 2500
PaeI GCATGC 1 cut(s) 2203
PasI CCCWGGG 1 cut(s) 1735
PceI AGGCCT 2 cut(s) 342, 2300
PciI ACATGT 1 cut(s) 2496
PctI GAATGC 1 cut(s) 1152
PflMI CCANNNNNTGG 1 cut(s) 2001
PleI GAGTC 8 cut(s) 226, 359, 391, 550, 551, 756, 2253, 2283
PpsI GAGTC 8 cut(s) 226, 359, 391, 550, 551, 756, 2253, 2283
PpuMI RGGWCCY 2 cut(s) 2167, 2439
PscI ACATGT 1 cut(s) 2496
PshBI ATTAAT 2 cut(s) 1359, 2232
Psp124BI GAGCTC 3 cut(s) 913, 970, 1101
Psp1406I AACGTT 1 cut(s) 2329
Psp5II RGGWCCY 2 cut(s) 2167, 2439
Psp6I CCWGG 4 cut(s) 1435, 1657, 1734, 2163
PspGI CCWGG 4 cut(s) 1435, 1657, 1734, 2163
PspN4I GGNNCC 9 cut(s) 685, 920, 1286, 1557, 1801, 1937, 2162, 2413, 2440
PspPI GGNCC 8 cut(s) 919, 1782, 1790, 1799, 1951, 2167, 2412, 2439
PspPPI RGGWCCY 2 cut(s) 2167, 2439
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 5 cut(s) 433, 759, 1759, 1874, 2483
RsaNI GTAC 5 cut(s) 432, 758, 1758, 1873, 2482
RseI CAYNNNNRTG 4 cut(s) 96, 569, 1752, 2309
SacI GAGCTC 3 cut(s) 913, 970, 1101
SaqAI TTAA 5 cut(s) 1274, 1347, 1359, 2103, 2232
Sau96I GGNCC 8 cut(s) 919, 1782, 1790, 1799, 1951, 2167, 2412, 2439
ScaI AGTACT 1 cut(s) 433
SchI GAGTC 8 cut(s) 227, 359, 392, 551, 551, 757, 2253, 2284
ScrFI CCNGG 4 cut(s) 1437, 1659, 1736, 2165
SfcI CTRYAG 1 cut(s) 1291
SinI GGWCC 6 cut(s) 1782, 1799, 1951, 2167, 2412, 2439
SmiMI CAYNNNNRTG 4 cut(s) 96, 569, 1752, 2309
SmlI CTYRAG 3 cut(s) 376, 400, 954
SmoI CTYRAG 3 cut(s) 376, 400, 954
SpeI ACTAGT 1 cut(s) 818
SphI GCATGC 1 cut(s) 2203
SseBI AGGCCT 2 cut(s) 342, 2300
SspI AATATT 3 cut(s) 450, 1306, 1357
SspMI CTAG 3 cut(s) 699, 819, 1368
SstI GAGCTC 3 cut(s) 913, 970, 1101
StuI AGGCCT 2 cut(s) 342, 2300
StyD4I CCNGG 4 cut(s) 1435, 1657, 1734, 2163
StyI CCWWGG 1 cut(s) 1221
TaaI ACNGT 7 cut(s) 352, 390, 683, 982, 1264, 1352, 2601
TaiI ACGT 3 cut(s) 676, 696, 2332
TaqI TCGA 9 cut(s) 356, 560, 964, 1101, 1144, 1723, 2469, 2560, 2584
TaqII GACCGA 2 cut(s) 1592, 1799
TatI WGTACW 3 cut(s) 431, 1872, 2481
TauI GCSGC 1 cut(s) 409
Tru1I TTAA 5 cut(s) 1274, 1347, 1359, 2103, 2232
Tru9I TTAA 5 cut(s) 1274, 1347, 1359, 2103, 2232
TscAI CASTG 3 cut(s) 949, 1267, 2142
TseFI GTSAC 4 cut(s) 384, 648, 971, 2276
TseI GCWGC 9 cut(s) 166, 227, 266, 271, 1226, 1326, 1432, 1591, 2354
Tsp45I GTSAC 4 cut(s) 384, 648, 971, 2276
TspGWI ACGGA 3 cut(s) 20, 199, 1928
TspRI CASTG 3 cut(s) 949, 1267, 2142
Van91I CCANNNNNTGG 1 cut(s) 2001
VneI GTGCAC 1 cut(s) 2500
VpaK11BI GGWCC 6 cut(s) 1782, 1799, 1951, 2167, 2412, 2439
VspI ATTAAT 2 cut(s) 1359, 2232
XapI RAATTY 3 cut(s) 77, 1663, 1740
XbaI TCTAGA 1 cut(s) 1367
XceI RCATGY 5 cut(s) 485, 1030, 2203, 2284, 2500
XcmI CCANNNNNNNNNTGG 1 cut(s) 2154
XmiI GTMKAC 1 cut(s) 2257
XspI CTAG 3 cut(s) 699, 819, 1368
ZrmI AGTACT 1 cut(s) 433
Zsp2I ATGCAT 1 cut(s) 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.