pycom02g20820

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004489
Physical Location & Seq
Reverse (-)
18845735 .. 18851908
6174 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20820.2

Sequence Viewer

Length: 2523 bp
ATGATGGATTTTGAGCCGTTACAACAGAAACCAGAATCCGCTGATGCCTGTGCCGATTTTGAGCGTGGATTTGAGGAATTCATGCGTGGGCACTTGGATGAATGTATGTCATTTGCATCATGTAGTTCTCCTCGTAATCCTGATGATGAAGATGACGAGGGTGAGCAGCTTGTGCGGAGGAGACCCAGGTTGGATTTGGAGGGTGATGATTTAGCAGAGTCATCTGCTGCACGGTGCCACCACTCGCGGATTTTGAGCCGTTGGGCTGCGCGGCAGGCGCAGGAGATGATAACAACGATTGAGAGGAGGAATCGTGAATCAGAGTTGATGGCACTTGCAGGGCTGCACACGGTTTCAATGCTTGACTCCTCATTCTTGAGGGAGTCGCAGTCTCCTACCTCAAGGCGGCAGGGGGCTGTGGAGAGGCCAAGCACTCAGGCATCTGCAATATTGCAAATGTGGAGGGAGTTAGAGGATGAGCATGTGTTGGATAGAGCGCGTGAAAGAGTGAGGGAAAGGTTAAGGCATAGGAGGAGTGTTGAGGCTAATACTAATGAGTCAACCACAAATATGTCTGAAAGTCGGGGGACTGAGAATCAGGGTAGTTTGGCAGATGCAAGTGAGAGTGAGAATGAGTTTGGAACTTGGTCACATGATCGAATAGGTTCACAACGTGAACATGGTGCTAATGACATTTCTAGTAGAGAGCAATCTCCTGATCTTGGCGAAGTCGAGAGGGAGAGAGTGAGACAGATTGTACGGGGATGGATGGAAACTGGGATTAGTGATCATTCGTCCAATGTTGCTCAGAGAAACACTAGTCCCAGATCAGAGTGGCTTGGTGAGACAGAGCGTGAAAGGGTGAGAATTGTTCGTGAGTGGGTACAAATGGCAAGTCAGCAAAGAGGAGCTCGTGGGGGCCAGAGGGATGAACAAGTCGCAAGTGGCGGTGCTCAAGTTGATCGAGCTCGTGATGGGACGGTTACTGACCATGAAGAAGGCCAAGCAGACCACATTCGCAGGGACATGCGGAGATTGCGTGGAAGACAAGCTCTAATTGATTTGCTTGTAAGGATTGAGAGGGAACGACAAAGGGAGCTCGAGTGTTTGGTGGAGCATCGGGCAGTCTCTGATTTTGCTCATCGTAACCGCATTCAGTCATTACTCAGAGGTAGATTCCTGCGAAATGAAAGACCTGTTGAAGAAGAAAGACCGCCTTCCGTGGCTGCTGGTGAATTAGTTCAGTTGAGACAGAGACACACAGTTTCTGGTTTAAGGGAAGGGTTCCGCTCCAGATTAGAAAATATTGTTCGTGGTCAAGATGTCCAACAGGAAAATAATGATCAATTGCAGCCTGGCAGTCAGGCAACTGACATCAATCAGTTGCCAGATCAAACGGTTAGCTTGGAGAGCAACACTACTGATGAGCTTCTAGATTGGCAAGAAACTACTAATCAAGGAGGGAATTGGCAGGAACCAGTGGCTGAAAATGACACACGAAACTGGCAGCAGCCAACCTTTGGTCAATTGAATGAATGGAGAGATGGTAATTCCGAAGATACGGCTGAAAATTGGCAAGGAAATTCAGTCGATAATTGGCCTGAGGAAACAACCAGAAACGTTGATGGAGAAGCAGATCATCGACAAGAAGCCCAGGGAATTTGGCATGAGAATGGTACTCGGGAGGCTGTTGCAAATTGGGCCGAAGGGCCTTCTGGTCCAGTGAGAAATCGCCGCTCCGTTCCAATTAGAAGATTCAATAGATTTCATCCGCCAGATGATGATAATGTGTACAGCATGGAACTCAGGGAACTTCTGAGCAGGAGAAGTGTCTCTAATCTTCTTCGCAGTGGGTTCCGTGAAAGTCTGGACCAACTGATTCAGTCATATGTTGAAAGGCGAAGTCATGCTCCCATTGATTGGGAATTGCATAGAAACTTGCCAACACCAACTCCAGCTTCACCAGAGCATGATCAGGAGCAACAGCGGGATGAGCAGAATGAAGATCAACGTGATGCCATCAACAGACCTTCACTTGTTCTACCATCTCCACCAGTGCCCCCACCACAGCCATTATGGCATCAGGACTTACATCAAACAGGCTGGTCTCGTCATAGCATGCATCGTTCAGAAATTGAATGGGAGATGATTAATGATCTCAGAGCAGACATGGCAAGACTACAGCAAGGCATGAGTCACATGCAGAGGATGTTGGAGGCCTGCATGGATATGCAATTGGAGTTGCAGCGATCTGTCAGACAGGAAGTCTCTGCAGCTTTGAATCGTTCATCTGGTGAAAAAGGTTTGGGCGCTGAGACATCAGAGGATGGGTCCAAATGGGGTCATGTGAGAAAAGGGACCTGTTGTGTGTGTTGTGATAGTCAAATCGATTCTCTCTTGTACAGATGCGGGCACATGTGCACTTGTTCAAAATGTGCGAACGAGTTGATTCGTGGCGGAGGGAAGTGCCCACTTTGTCGAGCACCGATTGTTGAGGTGATTCGAGCATACTCCATACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

841

Amino Acids

96.48

Weight (kDa)

5.34

Isoelectric Point (pI)

63.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 234
AccB7I CCANNNNNTGG 2 cut(s) 1520, 1920
AccBSI CCGCTC 2 cut(s) 1290, 1737
AccII CGCG 3 cut(s) 247, 271, 499
AclI AACGTT 1 cut(s) 1620
AcsI RAATTY 3 cut(s) 77, 1582, 1659
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 5 cut(s) 759, 885, 1678, 1793, 2402
AfiI CCNNNNNNNGG 4 cut(s) 405, 722, 1520, 1920
AflIII ACRYGT 1 cut(s) 2415
AgsI TTSAA 8 cut(s) 357, 1202, 1531, 1759, 1895, 2138, 2281, 2430
AhdI GACNNNNNGTC 1 cut(s) 2264
AhlI ACTAGT 1 cut(s) 818
AjnI CCWGG 3 cut(s) 185, 1354, 1653
AjuI GAANNNNNNNTTGG 2 cut(s) 1942, 1974
AluBI AGCT 9 cut(s) 169, 911, 968, 1052, 1099, 1404, 1429, 1958, 2276
AluI AGCT 9 cut(s) 169, 911, 968, 1052, 1099, 1404, 1429, 1958, 2276
Alw21I GWGCWC 6 cut(s) 913, 955, 970, 1101, 2423, 2485
Alw44I GTGCAC 1 cut(s) 2419
AlwNI CAGNNNCTG 3 cut(s) 1130, 1268, 1484
Ama87I CYCGRG 2 cut(s) 1100, 1680
AoxI GGCC 7 cut(s) 425, 919, 1000, 1598, 1701, 1709, 2217
ApaLI GTGCAC 1 cut(s) 2419
ApoI RAATTY 3 cut(s) 77, 1582, 1659
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AseI ATTAAT 1 cut(s) 2151
Asp700I GAANNNNTTC 2 cut(s) 664, 1239
AspLEI GCGC 4 cut(s) 271, 280, 499, 2312
AspS9I GGNCC 7 cut(s) 919, 1701, 1709, 1718, 1870, 2331, 2358
AsuHPI GGTGA 8 cut(s) 173, 215, 854, 874, 1244, 1953, 2306, 2509
AvaI CYCGRG 2 cut(s) 1100, 1680
AvaII GGWCC 4 cut(s) 1718, 1870, 2331, 2358
AxyI CCTNAGG 1 cut(s) 1602
BaeGI GKGCMC 5 cut(s) 93, 2061, 2415, 2423, 2471
BanI GGYRCC 1 cut(s) 234
BanII GRGCYC 3 cut(s) 913, 970, 1101
BauI CACGAG 2 cut(s) 912, 969
BbsI GAAGAC 1 cut(s) 1051
Bbv12I GWGCWC 6 cut(s) 913, 955, 970, 1101, 2423, 2485
BccI CCATC 9 cut(s) 322, 759, 763, 968, 1538, 1619, 2027, 2053, 2321
BceAI ACGGC 2 cut(s) 243, 1578
BciT130I CCWGG 3 cut(s) 187, 1356, 1655
BclI TGATCA 3 cut(s) 787, 1342, 1972
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 3 cut(s) 699, 819, 1433
BfmI CTRYAG 2 cut(s) 2180, 2271
BfoI RGCGCY 1 cut(s) 2313
BglI GCCNNNNNGGC 1 cut(s) 2077
Bme1390I CCNGG 3 cut(s) 187, 1356, 1655
Bme18I GGWCC 4 cut(s) 1718, 1870, 2331, 2358
BmeRI GACNNNNNGTC 1 cut(s) 2264
BmeT110I CYCGRG 2 cut(s) 1100, 1680
BmgT120I GGNCC 7 cut(s) 919, 1701, 1709, 1718, 1870, 2331, 2358
BmiI GGNNCC 7 cut(s) 236, 920, 1286, 1476, 1856, 2332, 2359
BmrFI CCNGG 3 cut(s) 187, 1356, 1655
BmrI ACTGGG 1 cut(s) 786
BmsI GCATC 9 cut(s) 34, 125, 449, 604, 1126, 2005, 2089, 2131, 2396
BmuI ACTGGG 1 cut(s) 786
BpiI GAAGAC 1 cut(s) 1051
BpmI CTGGAG 2 cut(s) 1276, 1938
BpuEI CTTGAG 3 cut(s) 385, 397, 939
Bsa29I ATCGAT 1 cut(s) 2388
BsaI GGTCTC 2 cut(s) 175, 2112
BsaJI CCNNGG 4 cut(s) 185, 1221, 1653, 1654
Bsc4I CCNNNNNNNGG 4 cut(s) 405, 722, 1520, 1920
Bse1I ACTGG 5 cut(s) 781, 1478, 1508, 1721, 2054
Bse21I CCTNAGG 1 cut(s) 1602
BseBI CCWGG 3 cut(s) 187, 1356, 1655
BseCI ATCGAT 1 cut(s) 2388
BseDI CCNNGG 4 cut(s) 185, 1221, 1653, 1654
BseGI GGATG 9 cut(s) 103, 481, 770, 774, 934, 1768, 1996, 2214, 2332
BseLI CCNNNNNNNGG 4 cut(s) 405, 722, 1520, 1920
BseMII CTCAG 9 cut(s) 449, 582, 821, 1180, 1593, 1808, 1819, 2173, 2304
BseNI ACTGG 5 cut(s) 781, 1478, 1508, 1721, 2054
BseRI GAGGAG 6 cut(s) 120, 193, 319, 358, 547, 921
BseSI GKGCMC 5 cut(s) 93, 2061, 2415, 2423, 2471
BsgI GTGCAG 2 cut(s) 213, 329
Bsh1236I CGCG 3 cut(s) 247, 271, 499
BshFI GGCC 7 cut(s) 427, 921, 1002, 1600, 1703, 1711, 2219
BshNI GGYRCC 1 cut(s) 234
BshVI ATCGAT 1 cut(s) 2388
BsiHKAI GWGCWC 6 cut(s) 913, 955, 970, 1101, 2423, 2485
BsiHKCI CYCGRG 2 cut(s) 1100, 1680
BslFI GGGAC 5 cut(s) 601, 807, 991, 1037, 2371
BslI CCNNNNNNNGG 4 cut(s) 405, 722, 1520, 1920
BsmFI GGGAC 5 cut(s) 601, 807, 991, 1037, 2371
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 7 cut(s) 427, 921, 1002, 1600, 1703, 1711, 2219
Bso31I GGTCTC 2 cut(s) 175, 2112
BsoBI CYCGRG 2 cut(s) 1100, 1680
Bsp1407I TGTACA 2 cut(s) 1791, 2400
BspANI GGCC 7 cut(s) 427, 921, 1002, 1600, 1703, 1711, 2219
BspCNI CTCAG 9 cut(s) 448, 583, 820, 1179, 1594, 1809, 1818, 2172, 2305
BspDI ATCGAT 1 cut(s) 2388
BspFNI CGCG 3 cut(s) 247, 271, 499
BspLI GGNNCC 7 cut(s) 236, 920, 1286, 1476, 1856, 2332, 2359
BspMAI CTGCAG 1 cut(s) 2275
BspT107I GGYRCC 1 cut(s) 234
BspTNI GGTCTC 2 cut(s) 175, 2112
BsrBI CCGCTC 2 cut(s) 1290, 1737
BsrGI TGTACA 2 cut(s) 1791, 2400
BsrI ACTGG 5 cut(s) 781, 1478, 1508, 1721, 2054
BssECI CCNNGG 4 cut(s) 185, 1221, 1653, 1654
BssSI CACGAG 2 cut(s) 912, 969
Bst2BI CACGAG 2 cut(s) 912, 969
Bst2UI CCWGG 3 cut(s) 187, 1356, 1655
Bst4CI ACNGT 6 cut(s) 234, 352, 982, 1264, 1399, 2520
BstAPI GCANNNNNTGC 1 cut(s) 172
BstAUI TGTACA 2 cut(s) 1791, 2400
BstC8I GCNNGC 4 cut(s) 276, 2120, 2221, 2411
BstDEI CTNAG 9 cut(s) 435, 591, 807, 1166, 1602, 1805, 1817, 2159, 2313
BstDSI CCRYGG 1 cut(s) 1221
BstF5I GGATG 9 cut(s) 103, 481, 770, 774, 934, 1768, 1996, 2214, 2332
BstFNI CGCG 3 cut(s) 247, 271, 499
BstH2I RGCGCY 1 cut(s) 2313
BstHHI GCGC 4 cut(s) 271, 280, 499, 2312
BstMWI GCNNNNNNNGC 9 cut(s) 172, 275, 277, 1036, 1410, 1700, 1993, 2077, 2171
BstNI CCWGG 3 cut(s) 187, 1356, 1655
BstNSI RCATGY 5 cut(s) 485, 1030, 2122, 2203, 2419
BstSCI CCNGG 3 cut(s) 185, 1354, 1653
BstSFI CTRYAG 2 cut(s) 2180, 2271
BstSLI GKGCMC 5 cut(s) 93, 2061, 2415, 2423, 2471
BstUI CGCG 3 cut(s) 247, 271, 499
BstV2I GAAGAC 1 cut(s) 1051
Bsu15I ATCGAT 1 cut(s) 2388
Bsu36I CCTNAGG 1 cut(s) 1602
BsuRI GGCC 7 cut(s) 427, 921, 1002, 1600, 1703, 1711, 2219
BsuTUI ATCGAT 1 cut(s) 2388
BtgI CCRYGG 1 cut(s) 1221
BtsCI GGATG 9 cut(s) 103, 481, 770, 774, 934, 1768, 1996, 2214, 2332
BtsI GCAGTG 1 cut(s) 1855
BtsIMutI CAGTG 4 cut(s) 1485, 1728, 1855, 2061
Cac8I GCNNGC 4 cut(s) 276, 2120, 2221, 2411
CaiI CAGNNNCTG 3 cut(s) 1130, 1268, 1484
CfoI GCGC 4 cut(s) 271, 280, 499, 2312
Cfr13I GGNCC 7 cut(s) 919, 1701, 1709, 1718, 1870, 2331, 2358
ClaI ATCGAT 1 cut(s) 2388
Csp6I GTAC 5 cut(s) 758, 884, 1677, 1792, 2401
CviQI GTAC 5 cut(s) 758, 884, 1677, 1792, 2401
DdeI CTNAG 9 cut(s) 435, 591, 807, 1166, 1602, 1805, 1817, 2159, 2313
DraIII CACNNNGTG 1 cut(s) 674
DriI GACNNNNNGTC 1 cut(s) 2264
Eam1105I GACNNNNNGTC 1 cut(s) 2264
EciI GGCGGA 2 cut(s) 1761, 2472
Ecl136II GAGCTC 3 cut(s) 911, 968, 1099
Eco147I AGGCCT 1 cut(s) 2219
Eco24I GRGCYC 3 cut(s) 913, 970, 1101
Eco31I GGTCTC 2 cut(s) 175, 2112
Eco47I GGWCC 4 cut(s) 1718, 1870, 2331, 2358
Eco53kI GAGCTC 3 cut(s) 911, 968, 1099
Eco81I CCTNAGG 1 cut(s) 1602
Eco88I CYCGRG 2 cut(s) 1100, 1680
EcoICRI GAGCTC 3 cut(s) 911, 968, 1099
EcoO109I RGGNCCY 2 cut(s) 1709, 2358
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 3 cut(s) 185, 1354, 1653
EcoT22I ATGCAT 1 cut(s) 2124
EcoT38I GRGCYC 3 cut(s) 913, 970, 1101
FalI AAGNNNNNCTT 2 cut(s) 1201, 1233
FaqI GGGAC 5 cut(s) 601, 807, 991, 1037, 2371
FauI CCCGC 2 cut(s) 1980, 2402
FauNDI CATATG 1 cut(s) 1888
FbaI TGATCA 3 cut(s) 787, 1342, 1972
FokI GGATG 9 cut(s) 110, 488, 777, 781, 941, 1755, 2003, 2221, 2339
FriOI GRGCYC 3 cut(s) 913, 970, 1101
FspBI CTAG 3 cut(s) 699, 819, 1433
GlaI GCGC 4 cut(s) 270, 279, 498, 2311
GsuI CTGGAG 2 cut(s) 1276, 1938
HaeII RGCGCY 1 cut(s) 2313
HaeIII GGCC 7 cut(s) 427, 921, 1002, 1600, 1703, 1711, 2219
HhaI GCGC 4 cut(s) 271, 280, 499, 2312
Hin6I GCGC 4 cut(s) 269, 278, 497, 2310
HinP1I GCGC 4 cut(s) 269, 278, 497, 2310
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HphI GGTGA 8 cut(s) 173, 215, 854, 874, 1244, 1953, 2306, 2509
Hpy166II GTNNAC 5 cut(s) 561, 668, 677, 1792, 2421
Hpy8I GTNNAC 5 cut(s) 561, 668, 677, 1792, 2421
HpyAV CCTTC 6 cut(s) 992, 1227, 1274, 1700, 1722, 2040
HpyCH4III ACNGT 6 cut(s) 234, 352, 982, 1264, 1399, 2520
HpyCH4IV ACGT 3 cut(s) 673, 1620, 2011
HpyF10VI GCNNNNNNNGC 9 cut(s) 172, 275, 277, 1036, 1410, 1700, 1993, 2077, 2171
HpyF3I CTNAG 9 cut(s) 435, 591, 807, 1166, 1602, 1805, 1817, 2159, 2313
HpySE526I ACGT 3 cut(s) 673, 1620, 2011
HspAI GCGC 4 cut(s) 269, 278, 497, 2310
Ksp22I TGATCA 3 cut(s) 787, 1342, 1972
LmnI GCTCC 7 cut(s) 908, 1096, 1114, 1295, 1742, 1915, 1978
LweI GCATC 9 cut(s) 34, 125, 449, 604, 1126, 2005, 2089, 2131, 2396
MaeI CTAG 3 cut(s) 699, 819, 1433
MaeII ACGT 3 cut(s) 673, 1620, 2011
MaeIII GTNAC 5 cut(s) 18, 648, 982, 1145, 2195
MbiI CCGCTC 2 cut(s) 1290, 1737
MfeI CAATTG 3 cut(s) 1346, 1526, 2234
MlyI GAGTC 5 cut(s) 227, 359, 392, 566, 2203
MmeI TCCRAC 4 cut(s) 171, 468, 1351, 2193
Mph1103I ATGCAT 1 cut(s) 2124
MroXI GAANNNNTTC 2 cut(s) 664, 1239
MseI TTAA 3 cut(s) 521, 1274, 2151
MslI CAYNNNNRTG 4 cut(s) 96, 569, 1671, 2228
MspA1I CMGCKG 2 cut(s) 41, 1987
MspR9I CCNGG 3 cut(s) 187, 1356, 1655
MunI CAATTG 3 cut(s) 1346, 1526, 2234
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 3 cut(s) 187, 1356, 1655
MvnI CGCG 3 cut(s) 247, 271, 499
MwoI GCNNNNNNNGC 9 cut(s) 172, 275, 277, 1036, 1410, 1700, 1993, 2077, 2171
NdeI CATATG 1 cut(s) 1888
NlaIV GGNNCC 7 cut(s) 236, 920, 1286, 1476, 1856, 2332, 2359
NmuCI GTSAC 2 cut(s) 648, 2195
NsiI ATGCAT 1 cut(s) 2124
NspI RCATGY 5 cut(s) 485, 1030, 2122, 2203, 2419
PaeI GCATGC 1 cut(s) 2122
PaeR7I CTCGAG 1 cut(s) 1100
PasI CCCWGGG 1 cut(s) 1654
PceI AGGCCT 1 cut(s) 2219
PciI ACATGT 1 cut(s) 2415
PctI GAATGC 1 cut(s) 1152
PdmI GAANNNNTTC 2 cut(s) 664, 1239
PflMI CCANNNNNTGG 2 cut(s) 1520, 1920
PleI GAGTC 5 cut(s) 226, 359, 391, 565, 2202
PpsI GAGTC 5 cut(s) 226, 359, 391, 565, 2202
PpuMI RGGWCCY 1 cut(s) 2358
PscI ACATGT 1 cut(s) 2415
PshBI ATTAAT 1 cut(s) 2151
Psp124BI GAGCTC 3 cut(s) 913, 970, 1101
Psp1406I AACGTT 1 cut(s) 1620
Psp5II RGGWCCY 1 cut(s) 2358
Psp6I CCWGG 3 cut(s) 185, 1354, 1653
PspGI CCWGG 3 cut(s) 185, 1354, 1653
PspN4I GGNNCC 7 cut(s) 236, 920, 1286, 1476, 1856, 2332, 2359
PspPI GGNCC 7 cut(s) 919, 1701, 1709, 1718, 1870, 2331, 2358
PspPPI RGGWCCY 1 cut(s) 2358
PspXI VCTCGAGB 1 cut(s) 1100
PstI CTGCAG 1 cut(s) 2275
PstNI CAGNNNCTG 3 cut(s) 1130, 1268, 1484
RsaI GTAC 5 cut(s) 759, 885, 1678, 1793, 2402
RsaNI GTAC 5 cut(s) 758, 884, 1677, 1792, 2401
RseI CAYNNNNRTG 4 cut(s) 96, 569, 1671, 2228
SacI GAGCTC 3 cut(s) 913, 970, 1101
SaqAI TTAA 3 cut(s) 521, 1274, 2151
Sau96I GGNCC 7 cut(s) 919, 1701, 1709, 1718, 1870, 2331, 2358
SchI GAGTC 5 cut(s) 227, 359, 392, 566, 2203
ScrFI CCNGG 3 cut(s) 187, 1356, 1655
SfaNI GCATC 9 cut(s) 34, 125, 449, 604, 1126, 2005, 2089, 2131, 2396
SfcI CTRYAG 2 cut(s) 2180, 2271
Sfr274I CTCGAG 1 cut(s) 1100
SinI GGWCC 4 cut(s) 1718, 1870, 2331, 2358
SlaI CTCGAG 1 cut(s) 1100
SmiMI CAYNNNNRTG 4 cut(s) 96, 569, 1671, 2228
SmlI CTYRAG 4 cut(s) 376, 400, 954, 1100
SmoI CTYRAG 4 cut(s) 376, 400, 954, 1100
SpeI ACTAGT 1 cut(s) 818
SphI GCATGC 1 cut(s) 2122
SseBI AGGCCT 1 cut(s) 2219
SspI AATATT 2 cut(s) 450, 1306
SspMI CTAG 3 cut(s) 699, 819, 1433
SstI GAGCTC 3 cut(s) 913, 970, 1101
StuI AGGCCT 1 cut(s) 2219
StyD4I CCNGG 3 cut(s) 185, 1354, 1653
TaaI ACNGT 6 cut(s) 234, 352, 982, 1264, 1399, 2520
TaiI ACGT 3 cut(s) 676, 1623, 2014
TaqI TCGA 9 cut(s) 658, 732, 964, 1101, 1590, 1642, 2388, 2479, 2503
TatI WGTACW 2 cut(s) 1791, 2400
TauI GCSGC 3 cut(s) 274, 409, 1737
Tru1I TTAA 3 cut(s) 521, 1274, 2151
Tru9I TTAA 3 cut(s) 521, 1274, 2151
TscAI CASTG 4 cut(s) 1485, 1728, 1855, 2061
TseFI GTSAC 2 cut(s) 648, 2195
Tsp45I GTSAC 2 cut(s) 648, 2195
TspGWI ACGGA 3 cut(s) 1210, 1729, 1847
TspRI CASTG 4 cut(s) 1485, 1728, 1855, 2061
Van91I CCANNNNNTGG 2 cut(s) 1520, 1920
VneI GTGCAC 1 cut(s) 2419
VpaK11BI GGWCC 4 cut(s) 1718, 1870, 2331, 2358
VspI ATTAAT 1 cut(s) 2151
XapI RAATTY 3 cut(s) 77, 1582, 1659
XbaI TCTAGA 1 cut(s) 1432
XceI RCATGY 5 cut(s) 485, 1030, 2122, 2203, 2419
XcmI CCANNNNNNNNNTGG 2 cut(s) 193, 2073
XhoI CTCGAG 1 cut(s) 1100
XmnI GAANNNNTTC 2 cut(s) 664, 1239
XspI CTAG 3 cut(s) 699, 819, 1433
Zsp2I ATGCAT 1 cut(s) 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.