Rmu_sc0001453.1_g000003

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001453.1
OG0004489
Physical Location & Seq
Forward (+)
23203 .. 29177
5975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001453.1_g000003.1.cds

Sequence Viewer

Length: 2679 bp
atgacggactttgagccgctgcaacagaagccggaatcggccgatgcctgcggtgatttcgagactggatatgaggaattcatgcgtgggcacttggatgaatgtatgtcgtttgcttcatgtagctctccgcggaatcctgatgatgaggacgatgagggggagcagcttgttcggaggagacggaggttggatttggaggggcatgatttggcggagtcgtctgctgctgcgcggcgccaccactcgcggattttgaccagttgggcggcgcggcaggcgcaggagatgattaccaccattgagaggaggaatcgggagtcggagctgatggcgcttgcggggttgcatacggtttcgatgctcgactcctccttcttgagggagtcacagtccccgacgtcgaggcggcaggggactgtggagaggccgagcactcaggcgtctgcaattttgcaaatgtggcgggagctggaggatgagcatttgttgaatagagcacgtgggagggtgagggaaaggttgaggcagcagaggaatgttgagtctaatactaatgagttgaacacaaatagatctgatagtcgggggagtgagaatcctgggagtttggtggatgcgagtgagagtgaaaatgagtatgggacgtggtcacatgatcagatgggttcaccaaacgaacgaggtgataataatgcatctagcagggagcaatctcccgatcttggcgaagttgagagggagagagtgaggcagattgtacggggatggatggaaactggcattactgatcattcatccaatgttgctccaagaaatagtagtcctagggcagagtggcttggtgagacggagcgggaaagggtgagaatcgtccgggagtgggtgcaaatggctagtcagcagagaggaggtcgtggaggccgacgggaagatcacgtcaccaatggtggtgctcaagttggtatagtgcgtgatgagtcagctgctgaccaagaagagagccaaccagagcacattcgccgggatatgttgaggttgcgtggaagacaagctataattgatttgcttgtgagaattgagagggaaagacaaagggagctccagtctttggtggagcatcgagctgtctctgattttgctcatcgcaaccgcattcagtcattactcagaggtagattcttgcgaaatgaaaggcctattgaagaagaaagaccaccttctatagccgcgggtgaattagtccaattgaggcagagacacactgtttctggtttaagaagcgatcatacccaaacaatcgcttctcaggatattcagcaggaaaaccgtgagcaatcccagcctgggagcgagggaggtgacaatcagttccctaatcaattggggaacttggataatgacacagctgtcgagcgacttgattggcaagaaactgctaatcaaggtgggaattggcaggaaccaattgctgaagatgagagacgtaactgggagcagacaacttttgatcaatttaacgattggagggaaggtaatgctgaagatatagttggaaattggcaagaaagttctgtcaataattggcctcagggaactgcgcgaaatgctgatgaagaaacaggtcatcaacaagaagctcagggtacatggcgtgagaatagttctcgggaagctgttgagaattggtcagaaagaccttcaggtcctgtgagaaatcgtcgttctggctcagttagaagattcaatagatttagtccaccagatgatgataccgtgtacagtatggaactcagggaacttctgagcaggagaagtgtttcgaatcttcttcgtagtggatttcgtgaaagtctggaccagctgatacaatcatatgttgaaaggcaaagtcgtgctcccattgattgggatttgcatagaaacttgccaacgccaactccggcttcaccagtgcaggaccaggaccgacagagggatgagctgaatgaagatcagcatgatgccatcaacagaccttcacgtgttctaccatctccaccagttcccccaccacaaccattatggcatcaggacttgcataacactggctggtctcgccatagcatgcatcgttcagaaattgaatgggagatgtttaatgatctcagatcagacatggcaagactccagcaaggcatgagccacatgcaaaggatgttggaggcctgcatggacatgcaattagagttgcaacgttctgtcagacaggaagtctcggcggctctcaatcgttcgcctggtgaaaaagggtcaagtgctgagacatcagaggatggttctaaatggggtcatgtaagaaaaggaacttgttgtgtttgttgtgatagtcacatcgattctctcttgtatagatgcggccatatgtgtacatgttcaaaatgtgcaaacgaattggttcgtggtggaggcaagtgcccactttgtcgagcgcctatagttgagaacacccacaccaatcctcctcaaaatcatccggaggggggcaccgggggagattatatcgatgggagcgagtacctcgccgtcgctgacgaggatcattacattaacaagcacaatttccggtgcgcagcctcatcaatgccgccaccaccacccaacacccaattctcttctccatacttctatcaactttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

892

Amino Acids

102.1

Weight (kDa)

5.36

Isoelectric Point (pI)

69.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1409, 1704
AatII GACGTC 1 cut(s) 404
Acc16I TGCGCA 1 cut(s) 2611
AccB1I GGYRCC 2 cut(s) 237, 2525
AccB7I CCANNNNNTGG 2 cut(s) 1111, 1908
AccBSI CCGCTC 1 cut(s) 856
AccII CGCG 6 cut(s) 133, 235, 250, 274, 1232, 1603
AccIII TCCGGA 1 cut(s) 2515
AclI AACGTT 1 cut(s) 2236
AclWI GGATC 1 cut(s) 2586
AcoI YGGCCR 2 cut(s) 39, 2398
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 3 cut(s) 1494, 1563, 1686
AcvI CACGTG 2 cut(s) 503, 2024
AcyI GRCGYC 3 cut(s) 238, 401, 443
AfaI GTAC 5 cut(s) 762, 1648, 1781, 2410, 2558
AfiI CCNNNNNNNGG 9 cut(s) 306, 381, 725, 883, 1111, 1347, 1908, 1975, 2522
AflIII ACRYGT 2 cut(s) 2023, 2411
AgsI TTSAA 7 cut(s) 493, 565, 1205, 1747, 1883, 2126, 2418
AhdI GACNNNNNGTC 1 cut(s) 2252
AjiI CACGTC 2 cut(s) 648, 940
AjnI CCWGG 4 cut(s) 601, 1345, 1962, 2278
AjuI GAANNNNNNNTTGG 6 cut(s) 1536, 1568, 1930, 1962, 2633, 2665
Alw21I GWGCWC 6 cut(s) 437, 502, 958, 1017, 1104, 1900
Alw26I GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2100, 2260, 2297
AlwI GGATC 1 cut(s) 2586
AlwNI CAGNNNCTG 2 cut(s) 989, 1709
Ama87I CYCGRG 1 cut(s) 1668
Aor13HI TCCGGA 1 cut(s) 2515
AoxI GGCC 7 cut(s) 39, 428, 922, 1196, 1586, 2205, 2398
ApeKI GCWGC 7 cut(s) 19, 166, 227, 230, 529, 986, 2612
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 2 cut(s) 883, 915
Asp700I GAANNNNTTC 2 cut(s) 1819, 2436
AspA2I CCTAGG 1 cut(s) 827
AspLEI GCGC 8 cut(s) 235, 240, 274, 283, 337, 1603, 2473, 2612
AspS9I GGNCC 4 cut(s) 1706, 1858, 1960, 1966
AsuC2I CCSGG 3 cut(s) 878, 1025, 2530
AsuII TTCGAA 1 cut(s) 1823
AvaI CYCGRG 1 cut(s) 1668
AvaII GGWCC 4 cut(s) 1706, 1858, 1960, 1966
AvrII CCTAGG 1 cut(s) 827
AxyI CCTNAGG 1 cut(s) 1590
BaeGI GKGCMC 3 cut(s) 93, 2459, 2528
BanI GGYRCC 2 cut(s) 237, 2525
BanII GRGCYC 1 cut(s) 1104
BarI GAAGNNNNNNTAC 2 cut(s) 1273, 1305
BbrPI CACGTG 2 cut(s) 503, 2024
BbsI GAAGAC 1 cut(s) 1054
Bbv12I GWGCWC 6 cut(s) 437, 502, 958, 1017, 1104, 1900
BbvI GCAGC 7 cut(s) 6, 178, 214, 217, 541, 973, 2624
BccI CCATC 8 cut(s) 325, 658, 762, 766, 2015, 2041, 2309, 2540
BceAI ACGGC 1 cut(s) 2549
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 4 cut(s) 603, 1347, 1964, 2280
BclI TGATCA 3 cut(s) 658, 790, 1510
BcnI CCSGG 3 cut(s) 878, 1025, 2530
BcoDI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2100, 2260, 2297
BfaI CTAG 3 cut(s) 702, 828, 897
BfmI CTRYAG 2 cut(s) 1224, 2475
BfoI RGCGCY 3 cut(s) 241, 338, 2474
BglII AGATCT 1 cut(s) 575
BlnI CCTAGG 1 cut(s) 827
Bme1390I CCNGG 7 cut(s) 603, 878, 1025, 1347, 1964, 2280, 2530
Bme18I GGWCC 4 cut(s) 1706, 1858, 1960, 1966
BmeRI GACNNNNNGTC 1 cut(s) 2252
BmeT110I CYCGRG 1 cut(s) 1668
BmgBI CACGTC 2 cut(s) 648, 940
BmgT120I GGNCC 4 cut(s) 1706, 1858, 1960, 1966
BmiI GGNNCC 3 cut(s) 239, 1464, 2527
BmrFI CCNGG 7 cut(s) 603, 878, 1025, 1347, 1964, 2280, 2530
BmrI ACTGGG 1 cut(s) 1501
BmsI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 1993, 2077, 2119, 2384
BmuI ACTGGG 1 cut(s) 1501
BpiI GAAGAC 1 cut(s) 1054
BpmI CTGGAG 3 cut(s) 494, 1088, 2153
Bpu10I CCTNAGC 1 cut(s) 1641
Bpu14I TTCGAA 1 cut(s) 1823
BpuEI CTTGAG 2 cut(s) 400, 942
BpuMI CCSGG 3 cut(s) 878, 1025, 2530
Bsa29I ATCGAT 2 cut(s) 2376, 2544
BsaAI YACGTR 2 cut(s) 503, 2024
BsaHI GRCGYC 3 cut(s) 238, 401, 443
BsaI GGTCTC 1 cut(s) 2100
BsaJI CCNNGG 6 cut(s) 131, 602, 827, 1230, 1346, 2529
BsaWI WCCGGW 2 cut(s) 2515, 2604
Bsc4I CCNNNNNNNGG 9 cut(s) 306, 381, 725, 883, 1111, 1347, 1908, 1975, 2522
Bse1I ACTGG 8 cut(s) 70, 261, 784, 1105, 1496, 1952, 2042, 2092
Bse21I CCTNAGG 1 cut(s) 1590
BseAI TCCGGA 1 cut(s) 2515
BseBI CCWGG 4 cut(s) 603, 1347, 1964, 2280
BseCI ATCGAT 2 cut(s) 2376, 2544
BseDI CCNNGG 6 cut(s) 131, 602, 827, 1230, 1346, 2529
BseLI CCNNNNNNNGG 9 cut(s) 306, 381, 725, 883, 1111, 1347, 1908, 1975, 2522
BseNI ACTGG 8 cut(s) 70, 261, 784, 1105, 1496, 1952, 2042, 2092
BseRI GAGGAG 5 cut(s) 193, 322, 361, 924, 2493
BseSI GKGCMC 3 cut(s) 93, 2459, 2528
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 7 cut(s) 6, 178, 214, 217, 541, 973, 2624
BseYI CCCAGC 1 cut(s) 1341
BsgI GTGCAG 1 cut(s) 1976
Bsh1236I CGCG 6 cut(s) 133, 235, 250, 274, 1232, 1603
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 7 cut(s) 41, 430, 924, 1198, 1588, 2207, 2400
BshNI GGYRCC 2 cut(s) 237, 2525
BshVI ATCGAT 2 cut(s) 2376, 2544
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 6 cut(s) 437, 502, 958, 1017, 1104, 1900
BsiHKCI CYCGRG 1 cut(s) 1668
BsiSI CCGG 7 cut(s) 32, 877, 1024, 1943, 2516, 2529, 2605
BslFI GGGAC 3 cut(s) 379, 430, 658
BslI CCNNNNNNNGG 9 cut(s) 306, 381, 725, 883, 1111, 1347, 1908, 1975, 2522
BsmAI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2100, 2260, 2297
BsmBI CGTCTC 3 cut(s) 175, 842, 1477
BsmFI GGGAC 3 cut(s) 379, 430, 658
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 7 cut(s) 41, 430, 924, 1198, 1588, 2207, 2400
Bso31I GGTCTC 1 cut(s) 2100
BsoBI CYCGRG 1 cut(s) 1668
Bsp119I TTCGAA 1 cut(s) 1823
Bsp1286I GDGCHC 9 cut(s) 93, 437, 502, 958, 1017, 1104, 1900, 2459, 2528
Bsp13I TCCGGA 1 cut(s) 2515
Bsp1407I TGTACA 2 cut(s) 1779, 2408
BspANI GGCC 7 cut(s) 41, 430, 924, 1198, 1588, 2207, 2400
BspDI ATCGAT 2 cut(s) 2376, 2544
BspEI TCCGGA 1 cut(s) 2515
BspFNI CGCG 6 cut(s) 133, 235, 250, 274, 1232, 1603
BspLI GGNNCC 3 cut(s) 239, 1464, 2527
BspPI GGATC 1 cut(s) 2586
BspT104I TTCGAA 1 cut(s) 1823
BspT107I GGYRCC 2 cut(s) 237, 2525
BspTNI GGTCTC 1 cut(s) 2100
BsrBI CCGCTC 1 cut(s) 856
BsrGI TGTACA 2 cut(s) 1779, 2408
BsrI ACTGG 8 cut(s) 70, 261, 784, 1105, 1496, 1952, 2042, 2092
BssECI CCNNGG 6 cut(s) 131, 602, 827, 1230, 1346, 2529
BssNI GRCGYC 3 cut(s) 238, 401, 443
BssT1I CCWWGG 1 cut(s) 827
Bst2UI CCWGG 4 cut(s) 603, 1347, 1964, 2280
Bst4CI ACNGT 7 cut(s) 355, 393, 421, 1267, 1331, 1777, 1784
Bst6I CTCTTC 2 cut(s) 993, 2659
BstACI GRCGYC 3 cut(s) 238, 401, 443
BstAUI TGTACA 2 cut(s) 1779, 2408
BstBAI YACGTR 2 cut(s) 503, 2024
BstBI TTCGAA 1 cut(s) 1823
BstC8I GCNNGC 5 cut(s) 49, 279, 339, 2108, 2209
BstDSI CCRYGG 2 cut(s) 131, 1230
BstENI CCTNNNNNAGG 1 cut(s) 379
BstFNI CGCG 6 cut(s) 133, 235, 250, 274, 1232, 1603
BstH2I RGCGCY 3 cut(s) 241, 338, 2474
BstHHI GCGC 8 cut(s) 235, 240, 274, 283, 337, 1603, 2473, 2612
BstMAI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1477, 2100, 2260, 2297
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 8 cut(s) 28, 278, 280, 334, 463, 1342, 1607, 2097
BstNI CCWGG 4 cut(s) 603, 1347, 1964, 2280
BstNSI RCATGY 4 cut(s) 2110, 2191, 2221, 2415
BstSCI CCNGG 7 cut(s) 601, 876, 1023, 1345, 1962, 2278, 2528
BstSFI CTRYAG 2 cut(s) 1224, 2475
BstSLI GKGCMC 3 cut(s) 93, 2459, 2528
BstUI CGCG 6 cut(s) 133, 235, 250, 274, 1232, 1603
BstV1I GCAGC 7 cut(s) 6, 178, 214, 217, 541, 973, 2624
BstV2I GAAGAC 1 cut(s) 1054
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 2 cut(s) 2376, 2544
Bsu36I CCTNAGG 1 cut(s) 1590
BsuRI GGCC 7 cut(s) 41, 430, 924, 1198, 1588, 2207, 2400
BsuTUI ATCGAT 2 cut(s) 2376, 2544
BtgI CCRYGG 2 cut(s) 131, 1230
BtgZI GCGATG 1 cut(s) 1130
BtrI CACGTC 2 cut(s) 648, 940
BtsIMutI CAGTG 3 cut(s) 1263, 1959, 2085
Cac8I GCNNGC 5 cut(s) 49, 279, 339, 2108, 2209
CaiI CAGNNNCTG 2 cut(s) 989, 1709
CfoI GCGC 8 cut(s) 235, 240, 274, 283, 337, 1603, 2473, 2612
Cfr13I GGNCC 4 cut(s) 1706, 1858, 1960, 1966
Cfr42I CCGCGG 2 cut(s) 134, 1233
ClaI ATCGAT 2 cut(s) 2376, 2544
CseI GACGC 1 cut(s) 432
Csp6I GTAC 5 cut(s) 761, 1647, 1780, 2409, 2557
CviQI GTAC 5 cut(s) 761, 1647, 1780, 2409, 2557
DinI GGCGCC 1 cut(s) 239
DrdI GACNNNNNNGTC 2 cut(s) 1409, 1704
DriI GACNNNNNGTC 1 cut(s) 2252
DseDI GACNNNNNNGTC 2 cut(s) 1409, 1704
EaeI YGGCCR 2 cut(s) 39, 2398
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 2 cut(s) 993, 2659
Eam1105I GACNNNNNGTC 1 cut(s) 2252
EarI CTCTTC 2 cut(s) 993, 2659
EciI GGCGGA 1 cut(s) 230
Ecl136II GAGCTC 1 cut(s) 1102
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 827
Eco147I AGGCCT 2 cut(s) 1198, 2207
Eco24I GRGCYC 1 cut(s) 1104
Eco31I GGTCTC 1 cut(s) 2100
Eco47I GGWCC 4 cut(s) 1706, 1858, 1960, 1966
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 1102
Eco57I CTGAAG 3 cut(s) 1494, 1563, 1686
Eco72I CACGTG 2 cut(s) 503, 2024
Eco81I CCTNAGG 1 cut(s) 1590
Eco88I CYCGRG 1 cut(s) 1668
EcoICRI GAGCTC 1 cut(s) 1102
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 1706
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 4 cut(s) 601, 1345, 1962, 2278
EcoT14I CCWWGG 1 cut(s) 827
EcoT22I ATGCAT 2 cut(s) 700, 2112
EcoT38I GRGCYC 1 cut(s) 1104
EgeI GGCGCC 1 cut(s) 239
EheI GGCGCC 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 827
Esp3I CGTCTC 3 cut(s) 175, 842, 1477
FalI AAGNNNNNCTT 2 cut(s) 1204, 1236
FaqI GGGAC 3 cut(s) 379, 430, 658
FauI CCCGC 4 cut(s) 334, 459, 849, 1225
FauNDI CATATG 2 cut(s) 1876, 2403
FbaI TGATCA 3 cut(s) 658, 790, 1510
FriOI GRGCYC 1 cut(s) 1104
FspBI CTAG 3 cut(s) 702, 828, 897
FspI TGCGCA 1 cut(s) 2611
GlaI GCGC 8 cut(s) 234, 239, 273, 282, 336, 1602, 2472, 2611
GsaI CCCAGC 1 cut(s) 1345
GsuI CTGGAG 3 cut(s) 494, 1088, 2153
HaeII RGCGCY 3 cut(s) 241, 338, 2474
HaeIII GGCC 7 cut(s) 41, 430, 924, 1198, 1588, 2207, 2400
HapII CCGG 7 cut(s) 32, 877, 1024, 1943, 2516, 2529, 2605
HgaI GACGC 1 cut(s) 432
HhaI GCGC 8 cut(s) 235, 240, 274, 283, 337, 1603, 2473, 2612
Hin1I GRCGYC 3 cut(s) 238, 401, 443
Hin6I GCGC 8 cut(s) 233, 238, 272, 281, 335, 1601, 2471, 2610
HinP1I GCGC 8 cut(s) 233, 238, 272, 281, 335, 1601, 2471, 2610
HpaII CCGG 7 cut(s) 32, 877, 1024, 1943, 2516, 2529, 2605
Hpy166II GTNNAC 4 cut(s) 671, 1760, 1780, 2409
Hpy8I GTNNAC 4 cut(s) 671, 1760, 1780, 2409
Hpy99I CGWCG 5 cut(s) 403, 406, 930, 1725, 2570
HpyAV CCTTC 5 cut(s) 385, 1230, 1526, 1710, 2028
HpyCH4III ACNGT 7 cut(s) 355, 393, 421, 1267, 1331, 1777, 1784
HpyCH4IV ACGT 7 cut(s) 401, 502, 647, 939, 1486, 2023, 2236
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 278, 280, 334, 463, 1342, 1607, 2097
HpySE526I ACGT 7 cut(s) 401, 502, 647, 939, 1486, 2023, 2236
Hsp92I GRCGYC 3 cut(s) 238, 401, 443
HspAI GCGC 8 cut(s) 233, 238, 272, 281, 335, 1601, 2471, 2610
KasI GGCGCC 1 cut(s) 237
Kpn2I TCCGGA 1 cut(s) 2515
Ksp22I TGATCA 3 cut(s) 658, 790, 1510
KspI CCGCGG 2 cut(s) 134, 1233
Lsp1109I GCAGC 7 cut(s) 6, 178, 214, 217, 541, 973, 2624
LweI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 1993, 2077, 2119, 2384
MaeI CTAG 3 cut(s) 702, 828, 897
MaeII ACGT 7 cut(s) 401, 502, 647, 939, 1486, 2023, 2236
MaeIII GTNAC 6 cut(s) 387, 651, 940, 1361, 1487, 2369
MbiI CCGCTC 1 cut(s) 856
MfeI CAATTG 3 cut(s) 1247, 1382, 1467
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 9 cut(s) 93, 437, 502, 958, 1017, 1104, 1900, 2459, 2528
Mly113I GGCGCC 1 cut(s) 238
MlyI GAGTC 7 cut(s) 227, 329, 362, 395, 554, 989, 2160
MmeI TCCRAC 4 cut(s) 171, 303, 1534, 2181
Mph1103I ATGCAT 2 cut(s) 700, 2112
MroI TCCGGA 1 cut(s) 2515
MroXI GAANNNNTTC 2 cut(s) 1819, 2436
MseI TTAA 5 cut(s) 1277, 1518, 2139, 2589, 2677
MslI CAYNNNNRTG 2 cut(s) 96, 2216
MspA1I CMGCKG 6 cut(s) 19, 133, 986, 1232, 1409, 1864
MspI CCGG 7 cut(s) 32, 877, 1024, 1943, 2516, 2529, 2605
MspR9I CCNGG 7 cut(s) 603, 878, 1025, 1347, 1964, 2280, 2530
MteI GCGCNGCGC 1 cut(s) 236
MunI CAATTG 3 cut(s) 1247, 1382, 1467
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 4 cut(s) 603, 1347, 1964, 2280
MvnI CGCG 6 cut(s) 133, 235, 250, 274, 1232, 1603
MwoI GCNNNNNNNGC 8 cut(s) 28, 278, 280, 334, 463, 1342, 1607, 2097
NarI GGCGCC 1 cut(s) 238
NciI CCSGG 3 cut(s) 878, 1025, 2530
NdeI CATATG 2 cut(s) 1876, 2403
NlaIV GGNNCC 3 cut(s) 239, 1464, 2527
NmeAIII GCCGAG 2 cut(s) 456, 2237
NmuCI GTSAC 5 cut(s) 387, 651, 940, 1361, 2369
NsbI TGCGCA 1 cut(s) 2611
NsiI ATGCAT 2 cut(s) 700, 2112
NspI RCATGY 4 cut(s) 2110, 2191, 2221, 2415
NspV TTCGAA 1 cut(s) 1823
PaeI GCATGC 1 cut(s) 2110
PceI AGGCCT 2 cut(s) 1198, 2207
PciI ACATGT 1 cut(s) 2411
PcsI WCGNNNNNNNCGW 2 cut(s) 922, 2550
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 2 cut(s) 1819, 2436
PfeI GAWTC 9 cut(s) 35, 136, 313, 598, 870, 1179, 1743, 1825, 2378
PflFI GACNNNGTC 1 cut(s) 649
PflMI CCANNNNNTGG 2 cut(s) 1111, 1908
PfoI TCCNGGA 1 cut(s) 876
PleI GAGTC 7 cut(s) 226, 328, 362, 394, 553, 988, 2160
PluTI GGCGCC 1 cut(s) 241
PmaCI CACGTG 2 cut(s) 503, 2024
PmlI CACGTG 2 cut(s) 503, 2024
PpsI GAGTC 7 cut(s) 226, 328, 362, 394, 553, 988, 2160
Ppu21I YACGTR 2 cut(s) 503, 2024
PpuMI RGGWCCY 1 cut(s) 1706
PscI ACATGT 1 cut(s) 2411
Psp124BI GAGCTC 1 cut(s) 1104
Psp1406I AACGTT 1 cut(s) 2236
Psp5II RGGWCCY 1 cut(s) 1706
Psp6I CCWGG 4 cut(s) 601, 1345, 1962, 2278
PspCI CACGTG 2 cut(s) 503, 2024
PspFI CCCAGC 1 cut(s) 1341
PspGI CCWGG 4 cut(s) 601, 1345, 1962, 2278
PspN4I GGNNCC 3 cut(s) 239, 1464, 2527
PspPI GGNCC 4 cut(s) 1706, 1858, 1960, 1966
PspPPI RGGWCCY 1 cut(s) 1706
PstNI CAGNNNCTG 2 cut(s) 989, 1709
PsuI RGATCY 1 cut(s) 575
PsyI GACNNNGTC 1 cut(s) 649
PvuII CAGCTG 3 cut(s) 986, 1409, 1864
RsaI GTAC 5 cut(s) 762, 1648, 1781, 2410, 2558
RsaNI GTAC 5 cut(s) 761, 1647, 1780, 2409, 2557
RseI CAYNNNNRTG 2 cut(s) 96, 2216
SacI GAGCTC 1 cut(s) 1104
SacII CCGCGG 2 cut(s) 134, 1233
SaqAI TTAA 5 cut(s) 1277, 1518, 2139, 2589, 2677
Sau96I GGNCC 4 cut(s) 1706, 1858, 1960, 1966
SchI GAGTC 7 cut(s) 227, 329, 362, 395, 554, 989, 2160
ScrFI CCNGG 7 cut(s) 603, 878, 1025, 1347, 1964, 2280, 2530
SduI GDGCHC 9 cut(s) 93, 437, 502, 958, 1017, 1104, 1900, 2459, 2528
SfaNI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 1993, 2077, 2119, 2384
SfcI CTRYAG 2 cut(s) 1224, 2475
SfoI GGCGCC 1 cut(s) 239
Sfr303I CCGCGG 2 cut(s) 134, 1233
SfuI TTCGAA 1 cut(s) 1823
SgrBI CCGCGG 2 cut(s) 134, 1233
SinI GGWCC 4 cut(s) 1706, 1858, 1960, 1966
SmiMI CAYNNNNRTG 2 cut(s) 96, 2216
SmlI CTYRAG 2 cut(s) 379, 957
SmoI CTYRAG 2 cut(s) 379, 957
SphI GCATGC 1 cut(s) 2110
SseBI AGGCCT 2 cut(s) 1198, 2207
SspDI GGCGCC 1 cut(s) 237
SspMI CTAG 3 cut(s) 702, 828, 897
SstI GAGCTC 1 cut(s) 1104
StuI AGGCCT 2 cut(s) 1198, 2207
StyD4I CCNGG 7 cut(s) 601, 876, 1023, 1345, 1962, 2278, 2528
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 7 cut(s) 355, 393, 421, 1267, 1331, 1777, 1784
TaiI ACGT 7 cut(s) 404, 505, 650, 942, 1489, 2026, 2239
TaqII GACCGA 1 cut(s) 1983
TatI WGTACW 2 cut(s) 1779, 2408
TauI GCSGC 9 cut(s) 19, 238, 272, 277, 412, 1232, 2264, 2400, 2629
TfiI GAWTC 9 cut(s) 35, 136, 313, 598, 870, 1179, 1743, 1825, 2378
Tru1I TTAA 5 cut(s) 1277, 1518, 2139, 2589, 2677
Tru9I TTAA 5 cut(s) 1277, 1518, 2139, 2589, 2677
TscAI CASTG 3 cut(s) 1270, 1959, 2092
TseFI GTSAC 5 cut(s) 387, 651, 940, 1361, 2369
TseI GCWGC 7 cut(s) 19, 166, 227, 230, 529, 986, 2612
Tsp45I GTSAC 5 cut(s) 387, 651, 940, 1361, 2369
TspDTI ATGAA 7 cut(s) 70, 108, 114, 786, 1206, 1629, 2004
TspGWI ACGGA 3 cut(s) 20, 199, 866
TspRI CASTG 3 cut(s) 1270, 1959, 2092
Tth111I GACNNNGTC 1 cut(s) 649
Van91I CCANNNNNTGG 2 cut(s) 1111, 1908
VpaK11BI GGWCC 4 cut(s) 1706, 1858, 1960, 1966
XagI CCTNNNNNAGG 1 cut(s) 379
XapI RAATTY 1 cut(s) 77
XceI RCATGY 4 cut(s) 2110, 2191, 2221, 2415
XcmI CCANNNNNNNNNTGG 1 cut(s) 2061
XmaJI CCTAGG 1 cut(s) 827
XmnI GAANNNNTTC 2 cut(s) 1819, 2436
XspI CTAG 3 cut(s) 702, 828, 897
ZraI GACGTC 1 cut(s) 402
Zsp2I ATGCAT 2 cut(s) 700, 2112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.