Rh1BG113500

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0004489
Physical Location & Seq
Forward (+)
19351196 .. 19356339
5144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG113500.1

Sequence Viewer

Length: 2604 bp
ATGACGGACTTTGAGCCGCTGCAACAGAAGCCGGAATCGGCCGATGCCTGCGGTGATTTCGAGACTGGATATGAGGAATTCATGCGGGGGCACTTGGATGAATGTATGTCATTTGCTTCATGTAGCTCTCCGAGGAATCCTGATGATGAGGACGATGAGGGGGAGCAGCTTGTTCGGAGGAGACGGAGGTTGGATTTGGAGGGGGATGATTTGGCGGAGTCGTCTGCTGCTGCGCGGCGCCACCATTCGCGGATTTTGACCCGTTGGGCGGCGCGGCAGGCGCAGGAGATGATAACCACCATTGAGAGGAGGAATCGGGAGTCGGAGCTGATGGCGCTTGCGGGGTTGCATACGGTTTCGATGCTCGACTCCTCCTTCTTGAGGGAGTCACAGTCCCCGACGTCGAGGCGGCAGGGGACTGTGGAGAGGCCGAGCACTCAGGCGTCTGCAATTTTGCAAATGTGGCGGGAGCTGGAGGATGAGCATTTGTTGAATAGAGCACGTGGGAGGGTGAGGGAAAGGTTGAGGCAGCAGAGGAATGTTGAGTCTAATACTAATGAGTTGACCACAAATAGATCTGATAGTCGGGGGAGTGAGAATCCTGGGAGTTTGGTGGATGCGAGTGAGAGTGAAAATGAGTATGGGACGTGGTCACATGATCAGATGGGTTCACCAAACGAACGAGGTGATAATAATGCATCTAGCAGGGAGCAATCTCCCGATCTTGGCGAAGTTGAGAGGGAGAGAGTGAGGCAGATTGTACGGGGATGGATGGAAACTGGCATTACTGATCATTCATCCAATGTTGCTCCAAGAAATAGTAGTCCTAGGGCAGAGTGGCTTGGTGAGACGGAGCGGGAAAGGGTGAGAATCGTCCGGGAGTGGGTGCAAATGGCTAGTCAGCAGAGAGGAGGTCGTGGAGGCCGACGGGAAGATCACGTCACCAATGGTGGTGCTCAAGTTGGTATAGTGCGTGATGAGTCAGCTGCTGACCAAGAAGAGAGCCAACCAGAGCACATTCGCCGGGATATGTTGAGGTTGCGTGGAAGACAAGCTATAATTGATTTGCTTGTGAGAATTGAGAGGGAAAGACAAAGGGAGCTCCAGTCTTTGGTGGAGCATCGAGCTGTCTCTGATTTTGCTCATCGCAACCGCATTCAGTCATTACTCAGAGGTAGATTCTTGCGAAATGAAAGGCCTATTGAAGAAGAAAGACCACCTTCTATAGCCGCGGGTGAATTAGTCCAATTGAGGCAGAGACACACTGTTTCTGGTTTAAGGGAAGGGTTCCGCTCCAGATTAGAAACTATAGTTCGTGGTCAAGTTGACGGCAATAATGATTCTCCGACTAACAGTAATGTTAATGATTCTAGAAGCGATCATACCCAAACAATCGCTTCTCAGGATATTCAGCAGGAAAACCGTGAGCAATCCCAGCCTGGGAGCGAGGGAGGTGACAATCAGTTCCCTAATCAATTGGGGAACTTGGAAAATGACACAGCTGTCGAGCGACTTGATTGGCAAGAAACTGCTAATCAAGGTGGGAATTGGCAGGAACCAATTGCTGAAGATGAGAGACGTAACTGGGAGCAGACAACTTTTGATCAATTTAACGATTGGAGGGAAGGTAATGCTGAAGATATAGTTGGAAATTGGCAAGAAAGTTCTGTCAATAATTGGCCTCAGGGAACTGCGCGAAATGCTGATGAAGAAACAGGTCATCAACACGAAGCTCAGGGTACATGGCGTGAGAATAGTTCTCGGGAAGCTGTTGAGAATTGGTCAGAAAGACCTTCAGGTCCTGTGAGAAATCGTCGTTCTGGCTCAGTTAGAAGATTCAATAGATTTAGTCCACCAGATGATGATACCGTGTACAGTATGGAACTCAGGGAACTTCTGAGCAGGAGAAGCGTTTCGAATCTTCTTCGTAGTGGATTTCGTGAAAGTCTGGACCAGCTGATACAATCATATGTTGAAAGGCAAAGTCGTGCTCCCATTGATTGGGATTTGCATAGAAACTTGCCAACACCAACTCCGGCTTCACCAGTGCAGGACCAGGACCGACAGAGGGATGAGCTGAATGAAGATCAGCATGATGCCATCAACAGACCTTCACGTGTTCTACCATCTCCACCAGTTCCCCCACCACAACCATTATGGCATCAGGACTTGCATAACACTGGCTGGTCTCGCCATAGCATGCATCGTTCAGAAATTGAATGGGAGATGTTTAATGATCTCAGATCAGACATGGCAAGACTCCAGCAAGGCATGAGCCACATGCAAAGGATGTTGGAGGCCTGCATGGATATGCAATTAGAGTTGCAACGTTCTGTCAGACAGGAAGTCTCGGCGGCTCTCAATCGTTCGCCTGGTGAAAAAGGGTCAAGTGCTGAGACATCAGAGGATGGTTCTAAATGGGGTCATGTAAGAAAAGGAACTTGTTGTGTTTGTTGTGATAGTCACATCGATTCTCTCTTGTATAGATGCGGGCATATGTGTACATGTTCAAAATGTGCAAACGAATTGGTTCGTGGTGGAGGCAAGTGCCCACTTTGTCGAGCGCCTATAGTTGAGGTGATCCGTGCATACTCCATACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

867

Amino Acids

99.42

Weight (kDa)

5.41

Isoelectric Point (pI)

68.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 812 - 859 5.8e-11 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1502, 1797
AatII GACGTC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 2 cut(s) 1111, 2001
AccBSI CCGCTC 2 cut(s) 856, 1293
AccII CGCG 5 cut(s) 235, 250, 274, 1232, 1696
AclI AACGTT 1 cut(s) 2329
AclWI GGATC 1 cut(s) 2575
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 3 cut(s) 1587, 1656, 1779
AcvI CACGTG 2 cut(s) 503, 2117
AcyI GRCGYC 3 cut(s) 238, 401, 443
AfaI GTAC 4 cut(s) 762, 1741, 1874, 2503
AfiI CCNNNNNNNGG 9 cut(s) 268, 306, 381, 725, 883, 1111, 1440, 2001, 2068
AflIII ACRYGT 2 cut(s) 2116, 2504
AgsI TTSAA 6 cut(s) 493, 1205, 1840, 1976, 2219, 2511
AhdI GACNNNNNGTC 1 cut(s) 2345
AjiI CACGTC 2 cut(s) 648, 940
AjnI CCWGG 4 cut(s) 601, 1438, 2055, 2371
AjuI GAANNNNNNNTTGG 4 cut(s) 1629, 1661, 2023, 2055
Alw21I GWGCWC 6 cut(s) 437, 502, 958, 1017, 1104, 1993
Alw26I GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1570, 2193, 2353, 2390
AlwI GGATC 1 cut(s) 2575
AlwNI CAGNNNCTG 2 cut(s) 989, 1802
Ama87I CYCGRG 1 cut(s) 1761
AoxI GGCC 6 cut(s) 39, 428, 922, 1196, 1679, 2298
ApeKI GCWGC 6 cut(s) 19, 166, 227, 230, 529, 986
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 2 cut(s) 883, 915
Asp700I GAANNNNTTC 2 cut(s) 1912, 2529
AspA2I CCTAGG 1 cut(s) 827
AspLEI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1696, 2566
AspS9I GGNCC 4 cut(s) 1799, 1951, 2053, 2059
AsuC2I CCSGG 2 cut(s) 878, 1025
AsuII TTCGAA 1 cut(s) 1916
AvaI CYCGRG 1 cut(s) 1761
AvaII GGWCC 4 cut(s) 1799, 1951, 2053, 2059
AvrII CCTAGG 1 cut(s) 827
AxyI CCTNAGG 1 cut(s) 1683
BaeGI GKGCMC 2 cut(s) 93, 2552
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 1104
BarI GAAGNNNNNNTAC 2 cut(s) 1366, 1398
BbrPI CACGTG 2 cut(s) 503, 2117
BbsI GAAGAC 1 cut(s) 1054
Bbv12I GWGCWC 6 cut(s) 437, 502, 958, 1017, 1104, 1993
BbvI GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
BccI CCATC 7 cut(s) 325, 658, 762, 766, 2108, 2134, 2402
BceAI ACGGC 1 cut(s) 1345
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 4 cut(s) 603, 1440, 2057, 2373
BclI TGATCA 3 cut(s) 658, 790, 1603
BcnI CCSGG 2 cut(s) 878, 1025
BcoDI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1570, 2193, 2353, 2390
BfaI CTAG 4 cut(s) 702, 828, 897, 1371
BfmI CTRYAG 4 cut(s) 1224, 1308, 2568, 2600
BfoI RGCGCY 3 cut(s) 241, 338, 2567
BglII AGATCT 1 cut(s) 575
BlnI CCTAGG 1 cut(s) 827
Bme1390I CCNGG 6 cut(s) 603, 878, 1025, 1440, 2057, 2373
Bme18I GGWCC 4 cut(s) 1799, 1951, 2053, 2059
BmeRI GACNNNNNGTC 1 cut(s) 2345
BmeT110I CYCGRG 1 cut(s) 1761
BmgBI CACGTC 2 cut(s) 648, 940
BmgT120I GGNCC 4 cut(s) 1799, 1951, 2053, 2059
BmiI GGNNCC 3 cut(s) 239, 1289, 1557
BmrFI CCNGG 6 cut(s) 603, 878, 1025, 1440, 2057, 2373
BmrI ACTGGG 1 cut(s) 1594
BmsI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 2086, 2170, 2212, 2477
BmuI ACTGGG 1 cut(s) 1594
BpiI GAAGAC 1 cut(s) 1054
BpmI CTGGAG 4 cut(s) 494, 1088, 1279, 2246
Bpu10I CCTNAGC 1 cut(s) 1734
Bpu14I TTCGAA 1 cut(s) 1916
BpuEI CTTGAG 2 cut(s) 400, 942
BpuMI CCSGG 2 cut(s) 878, 1025
Bsa29I ATCGAT 1 cut(s) 2469
BsaAI YACGTR 2 cut(s) 503, 2117
BsaHI GRCGYC 3 cut(s) 238, 401, 443
BsaI GGTCTC 1 cut(s) 2193
BsaJI CCNNGG 5 cut(s) 131, 602, 827, 1230, 1439
BsaXI ACNNNNNCTCC 8 cut(s) 377, 407, 1091, 1121, 1612, 1642, 2017, 2047
Bsc4I CCNNNNNNNGG 9 cut(s) 268, 306, 381, 725, 883, 1111, 1440, 2001, 2068
Bse1I ACTGG 7 cut(s) 70, 784, 1105, 1589, 2045, 2135, 2185
Bse21I CCTNAGG 1 cut(s) 1683
BseBI CCWGG 4 cut(s) 603, 1440, 2057, 2373
BseCI ATCGAT 1 cut(s) 2469
BseDI CCNNGG 5 cut(s) 131, 602, 827, 1230, 1439
BseLI CCNNNNNNNGG 9 cut(s) 268, 306, 381, 725, 883, 1111, 1440, 2001, 2068
BseNI ACTGG 7 cut(s) 70, 784, 1105, 1589, 2045, 2135, 2185
BseRI GAGGAG 4 cut(s) 193, 322, 361, 924
BseSI GKGCMC 2 cut(s) 93, 2552
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
BseYI CCCAGC 1 cut(s) 1434
BsgI GTGCAG 1 cut(s) 2069
Bsh1236I CGCG 5 cut(s) 235, 250, 274, 1232, 1696
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 6 cut(s) 41, 430, 924, 1198, 1681, 2300
BshNI GGYRCC 1 cut(s) 237
BshVI ATCGAT 1 cut(s) 2469
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 6 cut(s) 437, 502, 958, 1017, 1104, 1993
BsiHKCI CYCGRG 1 cut(s) 1761
BsiSI CCGG 4 cut(s) 32, 877, 1024, 2036
BslFI GGGAC 3 cut(s) 379, 430, 658
BslI CCNNNNNNNGG 9 cut(s) 268, 306, 381, 725, 883, 1111, 1440, 2001, 2068
BsmAI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1570, 2193, 2353, 2390
BsmBI CGTCTC 3 cut(s) 175, 842, 1570
BsmFI GGGAC 3 cut(s) 379, 430, 658
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 6 cut(s) 41, 430, 924, 1198, 1681, 2300
Bso31I GGTCTC 1 cut(s) 2193
BsoBI CYCGRG 1 cut(s) 1761
Bsp119I TTCGAA 1 cut(s) 1916
Bsp1286I GDGCHC 8 cut(s) 93, 437, 502, 958, 1017, 1104, 1993, 2552
Bsp1407I TGTACA 2 cut(s) 1872, 2501
BspANI GGCC 6 cut(s) 41, 430, 924, 1198, 1681, 2300
BspDI ATCGAT 1 cut(s) 2469
BspFNI CGCG 5 cut(s) 235, 250, 274, 1232, 1696
BspLI GGNNCC 3 cut(s) 239, 1289, 1557
BspPI GGATC 1 cut(s) 2575
BspT104I TTCGAA 1 cut(s) 1916
BspT107I GGYRCC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 2193
BsrBI CCGCTC 2 cut(s) 856, 1293
BsrGI TGTACA 2 cut(s) 1872, 2501
BsrI ACTGG 7 cut(s) 70, 784, 1105, 1589, 2045, 2135, 2185
BssECI CCNNGG 5 cut(s) 131, 602, 827, 1230, 1439
BssNI GRCGYC 3 cut(s) 238, 401, 443
BssT1I CCWWGG 1 cut(s) 827
Bst2UI CCWGG 4 cut(s) 603, 1440, 2057, 2373
Bst4CI ACNGT 9 cut(s) 355, 393, 421, 1267, 1355, 1424, 1870, 1877, 2601
Bst6I CTCTTC 1 cut(s) 993
BstACI GRCGYC 3 cut(s) 238, 401, 443
BstAUI TGTACA 2 cut(s) 1872, 2501
BstBAI YACGTR 2 cut(s) 503, 2117
BstBI TTCGAA 1 cut(s) 1916
BstC8I GCNNGC 6 cut(s) 49, 279, 339, 2201, 2302, 2492
BstDSI CCRYGG 1 cut(s) 1230
BstENI CCTNNNNNAGG 1 cut(s) 379
BstFNI CGCG 5 cut(s) 235, 250, 274, 1232, 1696
BstH2I RGCGCY 3 cut(s) 241, 338, 2567
BstHHI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1696, 2566
BstMAI GTCTC 9 cut(s) 56, 175, 842, 1135, 1252, 1570, 2193, 2353, 2390
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 9 cut(s) 28, 278, 280, 334, 463, 1435, 1700, 1908, 2190
BstNI CCWGG 4 cut(s) 603, 1440, 2057, 2373
BstNSI RCATGY 3 cut(s) 2203, 2284, 2508
BstSCI CCNGG 6 cut(s) 601, 876, 1023, 1438, 2055, 2371
BstSFI CTRYAG 4 cut(s) 1224, 1308, 2568, 2600
BstSLI GKGCMC 2 cut(s) 93, 2552
BstUI CGCG 5 cut(s) 235, 250, 274, 1232, 1696
BstV1I GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
BstV2I GAAGAC 1 cut(s) 1054
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 2469
Bsu36I CCTNAGG 1 cut(s) 1683
BsuRI GGCC 6 cut(s) 41, 430, 924, 1198, 1681, 2300
BsuTUI ATCGAT 1 cut(s) 2469
BtgI CCRYGG 1 cut(s) 1230
BtgZI GCGATG 1 cut(s) 1130
BtrI CACGTC 2 cut(s) 648, 940
BtsIMutI CAGTG 3 cut(s) 1263, 2052, 2178
Cac8I GCNNGC 6 cut(s) 49, 279, 339, 2201, 2302, 2492
CaiI CAGNNNCTG 2 cut(s) 989, 1802
CfoI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1696, 2566
Cfr13I GGNCC 4 cut(s) 1799, 1951, 2053, 2059
Cfr42I CCGCGG 1 cut(s) 1233
ClaI ATCGAT 1 cut(s) 2469
CseI GACGC 1 cut(s) 432
Csp6I GTAC 4 cut(s) 761, 1740, 1873, 2502
CviQI GTAC 4 cut(s) 761, 1740, 1873, 2502
DinI GGCGCC 1 cut(s) 239
DrdI GACNNNNNNGTC 2 cut(s) 1502, 1797
DriI GACNNNNNGTC 1 cut(s) 2345
DseDI GACNNNNNNGTC 2 cut(s) 1502, 1797
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 993
Eam1105I GACNNNNNGTC 1 cut(s) 2345
EarI CTCTTC 1 cut(s) 993
EciI GGCGGA 1 cut(s) 230
Ecl136II GAGCTC 1 cut(s) 1102
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 827
Eco147I AGGCCT 2 cut(s) 1198, 2300
Eco24I GRGCYC 1 cut(s) 1104
Eco31I GGTCTC 1 cut(s) 2193
Eco47I GGWCC 4 cut(s) 1799, 1951, 2053, 2059
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 1102
Eco57I CTGAAG 3 cut(s) 1587, 1656, 1779
Eco72I CACGTG 2 cut(s) 503, 2117
Eco81I CCTNAGG 1 cut(s) 1683
Eco88I CYCGRG 1 cut(s) 1761
EcoICRI GAGCTC 1 cut(s) 1102
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 1799
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 4 cut(s) 601, 1438, 2055, 2371
EcoT14I CCWWGG 1 cut(s) 827
EcoT22I ATGCAT 2 cut(s) 700, 2205
EcoT38I GRGCYC 1 cut(s) 1104
EgeI GGCGCC 1 cut(s) 239
EheI GGCGCC 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 827
Esp3I CGTCTC 3 cut(s) 175, 842, 1570
FalI AAGNNNNNCTT 2 cut(s) 1204, 1236
FaqI GGGAC 3 cut(s) 379, 430, 658
FauI CCCGC 6 cut(s) 78, 334, 459, 849, 1225, 2483
FauNDI CATATG 2 cut(s) 1969, 2496
FbaI TGATCA 3 cut(s) 658, 790, 1603
FriOI GRGCYC 1 cut(s) 1104
FspBI CTAG 4 cut(s) 702, 828, 897, 1371
GlaI GCGC 7 cut(s) 234, 239, 273, 282, 336, 1695, 2565
GsaI CCCAGC 1 cut(s) 1438
GsuI CTGGAG 4 cut(s) 494, 1088, 1279, 2246
HaeII RGCGCY 3 cut(s) 241, 338, 2567
HaeIII GGCC 6 cut(s) 41, 430, 924, 1198, 1681, 2300
HapII CCGG 4 cut(s) 32, 877, 1024, 2036
HgaI GACGC 1 cut(s) 432
HhaI GCGC 7 cut(s) 235, 240, 274, 283, 337, 1696, 2566
Hin1I GRCGYC 3 cut(s) 238, 401, 443
Hin6I GCGC 7 cut(s) 233, 238, 272, 281, 335, 1694, 2564
HinP1I GCGC 7 cut(s) 233, 238, 272, 281, 335, 1694, 2564
HincII GTYRAC 2 cut(s) 564, 1327
HindII GTYRAC 2 cut(s) 564, 1327
HpaII CCGG 4 cut(s) 32, 877, 1024, 2036
Hpy166II GTNNAC 6 cut(s) 564, 671, 1327, 1853, 1873, 2502
Hpy8I GTNNAC 6 cut(s) 564, 671, 1327, 1853, 1873, 2502
Hpy99I CGWCG 4 cut(s) 403, 406, 930, 1818
HpyAV CCTTC 6 cut(s) 385, 1230, 1277, 1619, 1803, 2121
HpyCH4III ACNGT 9 cut(s) 355, 393, 421, 1267, 1355, 1424, 1870, 1877, 2601
HpyCH4IV ACGT 7 cut(s) 401, 502, 647, 939, 1579, 2116, 2329
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 278, 280, 334, 463, 1435, 1700, 1908, 2190
HpySE526I ACGT 7 cut(s) 401, 502, 647, 939, 1579, 2116, 2329
Hsp92I GRCGYC 3 cut(s) 238, 401, 443
HspAI GCGC 7 cut(s) 233, 238, 272, 281, 335, 1694, 2564
KasI GGCGCC 1 cut(s) 237
Ksp22I TGATCA 3 cut(s) 658, 790, 1603
KspI CCGCGG 1 cut(s) 1233
Lsp1109I GCAGC 6 cut(s) 6, 178, 214, 217, 541, 973
LweI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 2086, 2170, 2212, 2477
MaeI CTAG 4 cut(s) 702, 828, 897, 1371
MaeII ACGT 7 cut(s) 401, 502, 647, 939, 1579, 2116, 2329
MaeIII GTNAC 6 cut(s) 387, 651, 940, 1454, 1580, 2462
MbiI CCGCTC 2 cut(s) 856, 1293
MfeI CAATTG 3 cut(s) 1247, 1475, 1560
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 8 cut(s) 93, 437, 502, 958, 1017, 1104, 1993, 2552
Mly113I GGCGCC 1 cut(s) 238
MlyI GAGTC 7 cut(s) 227, 329, 362, 395, 554, 989, 2253
MmeI TCCRAC 5 cut(s) 171, 303, 1370, 1627, 2274
Mph1103I ATGCAT 2 cut(s) 700, 2205
MroXI GAANNNNTTC 2 cut(s) 1912, 2529
MseI TTAA 4 cut(s) 1277, 1362, 1611, 2232
MslI CAYNNNNRTG 2 cut(s) 96, 2309
MspA1I CMGCKG 5 cut(s) 19, 986, 1232, 1502, 1957
MspI CCGG 4 cut(s) 32, 877, 1024, 2036
MspR9I CCNGG 6 cut(s) 603, 878, 1025, 1440, 2057, 2373
MteI GCGCNGCGC 1 cut(s) 236
MunI CAATTG 3 cut(s) 1247, 1475, 1560
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 4 cut(s) 603, 1440, 2057, 2373
MvnI CGCG 5 cut(s) 235, 250, 274, 1232, 1696
MwoI GCNNNNNNNGC 9 cut(s) 28, 278, 280, 334, 463, 1435, 1700, 1908, 2190
NarI GGCGCC 1 cut(s) 238
NciI CCSGG 2 cut(s) 878, 1025
NdeI CATATG 2 cut(s) 1969, 2496
NlaIV GGNNCC 3 cut(s) 239, 1289, 1557
NmeAIII GCCGAG 2 cut(s) 456, 2330
NmuCI GTSAC 5 cut(s) 387, 651, 940, 1454, 2462
NsiI ATGCAT 2 cut(s) 700, 2205
NspI RCATGY 3 cut(s) 2203, 2284, 2508
NspV TTCGAA 1 cut(s) 1916
PaeI GCATGC 1 cut(s) 2203
PceI AGGCCT 2 cut(s) 1198, 2300
PciI ACATGT 1 cut(s) 2504
PcsI WCGNNNNNNNCGW 1 cut(s) 922
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 2 cut(s) 1912, 2529
PflFI GACNNNGTC 1 cut(s) 649
PflMI CCANNNNNTGG 2 cut(s) 1111, 2001
PfoI TCCNGGA 1 cut(s) 876
PleI GAGTC 7 cut(s) 226, 328, 362, 394, 553, 988, 2253
PluTI GGCGCC 1 cut(s) 241
PmaCI CACGTG 2 cut(s) 503, 2117
PmlI CACGTG 2 cut(s) 503, 2117
PpsI GAGTC 7 cut(s) 226, 328, 362, 394, 553, 988, 2253
Ppu21I YACGTR 2 cut(s) 503, 2117
PpuMI RGGWCCY 1 cut(s) 1799
PscI ACATGT 1 cut(s) 2504
Psp124BI GAGCTC 1 cut(s) 1104
Psp1406I AACGTT 1 cut(s) 2329
Psp5II RGGWCCY 1 cut(s) 1799
Psp6I CCWGG 4 cut(s) 601, 1438, 2055, 2371
PspCI CACGTG 2 cut(s) 503, 2117
PspFI CCCAGC 1 cut(s) 1434
PspGI CCWGG 4 cut(s) 601, 1438, 2055, 2371
PspN4I GGNNCC 3 cut(s) 239, 1289, 1557
PspPI GGNCC 4 cut(s) 1799, 1951, 2053, 2059
PspPPI RGGWCCY 1 cut(s) 1799
PstNI CAGNNNCTG 2 cut(s) 989, 1802
PsuI RGATCY 1 cut(s) 575
PsyI GACNNNGTC 1 cut(s) 649
PvuII CAGCTG 3 cut(s) 986, 1502, 1957
RsaI GTAC 4 cut(s) 762, 1741, 1874, 2503
RsaNI GTAC 4 cut(s) 761, 1740, 1873, 2502
RseI CAYNNNNRTG 2 cut(s) 96, 2309
SacI GAGCTC 1 cut(s) 1104
SacII CCGCGG 1 cut(s) 1233
SaqAI TTAA 4 cut(s) 1277, 1362, 1611, 2232
Sau96I GGNCC 4 cut(s) 1799, 1951, 2053, 2059
SchI GAGTC 7 cut(s) 227, 329, 362, 395, 554, 989, 2253
ScrFI CCNGG 6 cut(s) 603, 878, 1025, 1440, 2057, 2373
SduI GDGCHC 8 cut(s) 93, 437, 502, 958, 1017, 1104, 1993, 2552
SfaNI GCATC 9 cut(s) 34, 351, 607, 707, 1129, 2086, 2170, 2212, 2477
SfcI CTRYAG 4 cut(s) 1224, 1308, 2568, 2600
SfoI GGCGCC 1 cut(s) 239
Sfr303I CCGCGG 1 cut(s) 1233
SfuI TTCGAA 1 cut(s) 1916
SgrBI CCGCGG 1 cut(s) 1233
SinI GGWCC 4 cut(s) 1799, 1951, 2053, 2059
SmiMI CAYNNNNRTG 2 cut(s) 96, 2309
SmlI CTYRAG 2 cut(s) 379, 957
SmoI CTYRAG 2 cut(s) 379, 957
SphI GCATGC 1 cut(s) 2203
SseBI AGGCCT 2 cut(s) 1198, 2300
SspDI GGCGCC 1 cut(s) 237
SspMI CTAG 4 cut(s) 702, 828, 897, 1371
SstI GAGCTC 1 cut(s) 1104
StuI AGGCCT 2 cut(s) 1198, 2300
StyD4I CCNGG 6 cut(s) 601, 876, 1023, 1438, 2055, 2371
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 9 cut(s) 355, 393, 421, 1267, 1355, 1424, 1870, 1877, 2601
TaiI ACGT 7 cut(s) 404, 505, 650, 942, 1582, 2119, 2332
TaqI TCGA 9 cut(s) 60, 359, 366, 404, 1123, 1506, 1916, 2469, 2560
TaqII GACCGA 1 cut(s) 2076
TatI WGTACW 2 cut(s) 1872, 2501
TauI GCSGC 7 cut(s) 19, 238, 272, 277, 412, 1232, 2357
Tru1I TTAA 4 cut(s) 1277, 1362, 1611, 2232
Tru9I TTAA 4 cut(s) 1277, 1362, 1611, 2232
TscAI CASTG 3 cut(s) 1270, 2052, 2185
TseFI GTSAC 5 cut(s) 387, 651, 940, 1454, 2462
TseI GCWGC 6 cut(s) 19, 166, 227, 230, 529, 986
Tsp45I GTSAC 5 cut(s) 387, 651, 940, 1454, 2462
TspDTI ATGAA 7 cut(s) 70, 108, 114, 786, 1206, 1722, 2097
TspGWI ACGGA 4 cut(s) 20, 199, 866, 2573
TspRI CASTG 3 cut(s) 1270, 2052, 2185
Tth111I GACNNNGTC 1 cut(s) 649
Van91I CCANNNNNTGG 2 cut(s) 1111, 2001
VpaK11BI GGWCC 4 cut(s) 1799, 1951, 2053, 2059
XagI CCTNNNNNAGG 1 cut(s) 379
XapI RAATTY 1 cut(s) 77
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 3 cut(s) 2203, 2284, 2508
XcmI CCANNNNNNNNNTGG 1 cut(s) 2154
XmaJI CCTAGG 1 cut(s) 827
XmnI GAANNNNTTC 2 cut(s) 1912, 2529
XspI CTAG 4 cut(s) 702, 828, 897, 1371
ZraI GACGTC 1 cut(s) 402
Zsp2I ATGCAT 2 cut(s) 700, 2205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.