pycom07g05860

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004489
Physical Location & Seq
Forward (+)
5142253 .. 5147808
5556 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g05860.6

Sequence Viewer

Length: 2523 bp
ATGACGGATTTTGAGCCGTTACAACAGAAGCCGGAATCCGCTGATGCCTGTGCCGATTTTGAGCGTGGATTTGAGGAATTCATGCGGGGGCACTTGGATGAATGTATGTCATTTGCATCATGTAGTTCTCCTCGTAATCCTGATGATGAAGATGACGAGGGTGAGCAGCTTGTGCGGAGGAGACGGAGGTTGGATTTGGAGGGTGACGATTTAGCAGAGTCATCCGCTGCACGGCGACACCACTCGCGGATCCTGAGCCGTTGGGCTGCGCAGCAGGCGCAGGAGATGATAACAACCATTGAGAGGAGGAATCGTGAATCAGAGTTGATGGCACTTGCAGGCCTGCACACGGTTTCGATGCTTGACTCCTCGTTCTTGAGGGAGTCACAGTCTCCTACCTCAAGGCGGCAGGGGGCTGTTGAGAGGCCAAGTACTCAGGCATCTGCAATATTGCAAATGTGGAGGGAATTAGAGGATGAGCATGTGTTGAATAGAGCGCGTGAAAGAGTGAGGGAAAGGTTGAGGCATAGGAGGAGTGTTGAGTCTAATACTAATGAGTCGACCACAAATATGTCTGATAGTCGGGGGAGTGAGAATCAAGGTAGTTTGGCGGACGCAAGTGAGAGTGAGAATGAGTTTGGAACTTGGTCACATGATCAAATAGGTTCACAACGTGAACACGGTGCCAATGACGTGTCTAGTAGAGAGCAATCTCCTGATCTTGGTGAAGTGGAGAGGGAGAGAGTGAGTCAGATTGTACGGGGATGGATGGAAACTGGCATTGGTGATCGTTCATCCAATGTTGCTCAGAGAAACACTAATCCCAGAGCAGAGTGGCTTGGTGAGACAGAGCGTGAAAGGGTGAGAATTGTTCGTGAGTGGGTGCAAATGGCAAGTCAGCAACGAGGAGCTCGTGGGGGCCAGAGGGATGATCAAGTTGCCAGTGGTGGTGCTCAAGTTGATCGAGCTCGTGACGGGACAGCTGCTGACCATGAAGAAGGCCAACCAGACCACATTCGCAGGGACATGCGGAGATTGCGTGGAAGACAAGCTCTAATTGATTTGCTTGTAAGGATTGAGAGGGAACGACAAAGGGAGCTCGATTGTTTGGTGGAGCATCGGGCTGTCTCTGATTTTGCTCATCGAAACCGCATTCAGTCATTACTCAGAGGTAGATTCCTGCGCAATGAAAGACCTATTGAAGAAGAAAGACCACCTTCCATGGCTGCTGGTGAATTAGTCCAGTTGAGACAGCGACACACTGTTTCTGGTTTAAGGGAAGGGTTCCGCTACAGATTAGAAAATATTGTTCGTGGTCAGGATGTCCAACAGGAAAGTAATGATCAATTGCAGCCTGGTAGTCAGGCAACTGACATCAATCAGTTGCCTGATCAAATGGGAAGCTTGGAGAGCAACACTACTGATGAGCGTCAAGATTGGCAAGAAACTGCTAATCAAGGAGGGAATTGGCAGGAACCAGTTGCTGAAGATGAGACACGAAACTTGGAGCAGCCAACTTTCGGTCAGTTGAATGAATGGAGAAATGGTAATGCCGAAGATACGGCTGAAAATTGGCAAGGAAATTCAGTCGATAATTGGCCTGAGGAAACACCCAGAAATGTTGATGGAGAAGCAGATAATCGACAAGAAGCCCAGGGAATGTGGCATGAGAATGGTACTCGGGATGCTGTTGGAAATTGGACCGAAGGGCCTTCTGGTCCCATGAGAAATCGCCGTTCAGTTCCAATTAGAAGATTCAATAGATTTCATCCGCCAGATGATGATAATGTGTACAGCATGGAACTCAGGGAACTTCTGAGCAGGAGAAGTGTCTCTAATCTTCTTCGCAGTGGGTTCCGTGAAAGTCTGGACCAACTGATTCAGTCATATGTTGAAAGGCAAAGTCATGCTCCCATTGATTGGGATTTGCATAGAAACTTGCCAACACCAATTCCAGCTTCACCAGATCATGATCAGGAGCAACAGCGGGATGGGCAGAATGACGATCAACATGATGCCATTAACAGACCTTCACTTGTTCTACCATCTCCACCAGTGCCCCCACCACAGCCATTATGGCATCAGGACCTACATCACACAGGCTGGTCTCGTCATAGCATGCATCGTTCAGAAATTGAATGGGAGATGATTAATGATCTCAGAGGAGACATGGGTAGACTCCAGCAAGGCATGAGTCACATGCAGAGGATGTTGGAGGCCTGCATGGATATGCAATTGGAGTTGCAACGTTCTGTTAGACAGGAAGTCTCTGCAGCTTTGAATCGTTCATCTGGTGAAAAAGGCTTGGGTGCTGAGACATCAGAGGATGGGTCCAAATGGGGTCATGTGAGAAAAGGGACCTGTTGTGTTTGTTGTGATAGTCAAATTGATTCTCTCTTGTACAGATGCGGGCACATGTGCACTTGTTCAAAATGTGCAAACGAGTTGATTCGTGGCGGAGGGAAGTGCCCGCTTTGTCGAGCACCGATTGTTGAGGTGATTCGAGCATACTCCATACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

841

Amino Acids

96.24

Weight (kDa)

5.33

Isoelectric Point (pI)

62.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 270, 1184
AccB1I GGYRCC 1 cut(s) 683
AccB7I CCANNNNNTGG 1 cut(s) 1920
AccI GTMKAC 2 cut(s) 560, 2176
AccII CGCG 2 cut(s) 247, 499
AclI AACGTT 1 cut(s) 2248
AclWI GGATC 2 cut(s) 244, 257
AcsI RAATTY 2 cut(s) 77, 1582
AcuI CTGAAG 1 cut(s) 1506
AdeI CACNNNGTG 1 cut(s) 674
AfaI GTAC 5 cut(s) 433, 759, 1678, 1793, 2402
AfiI CCNNNNNNNGG 7 cut(s) 231, 303, 349, 405, 722, 1520, 1920
AflIII ACRYGT 2 cut(s) 693, 2415
AgsI TTSAA 8 cut(s) 490, 1202, 1531, 1759, 1895, 2138, 2281, 2430
AhdI GACNNNNNGTC 1 cut(s) 2264
AjiI CACGTC 1 cut(s) 694
AjnI CCWGG 2 cut(s) 1354, 1653
AjuI GAANNNNNNNTTGG 4 cut(s) 765, 797, 1942, 1974
AluBI AGCT 9 cut(s) 169, 911, 968, 983, 1052, 1099, 1404, 1958, 2276
AluI AGCT 9 cut(s) 169, 911, 968, 983, 1052, 1099, 1404, 1958, 2276
Alw21I GWGCWC 6 cut(s) 913, 955, 970, 1101, 2423, 2485
Alw44I GTGCAC 1 cut(s) 2419
AlwI GGATC 2 cut(s) 244, 257
AlwNI CAGNNNCTG 2 cut(s) 986, 1484
Ama87I CYCGRG 1 cut(s) 1680
AoxI GGCC 7 cut(s) 340, 425, 919, 1000, 1598, 1709, 2217
ApaLI GTGCAC 1 cut(s) 2419
ApeKI GCWGC 9 cut(s) 166, 227, 266, 271, 983, 1226, 1351, 1510, 2273
ApoI RAATTY 2 cut(s) 77, 1582
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AseI ATTAAT 1 cut(s) 2151
AspLEI GCGC 4 cut(s) 271, 280, 499, 1185
AspS9I GGNCC 8 cut(s) 919, 1701, 1709, 1718, 1870, 2086, 2331, 2358
AvaI CYCGRG 1 cut(s) 1680
AvaII GGWCC 6 cut(s) 1701, 1718, 1870, 2086, 2331, 2358
AxyI CCTNAGG 1 cut(s) 1602
BaeGI GKGCMC 5 cut(s) 93, 2061, 2415, 2423, 2471
BamHI GGATCC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 683
BanII GRGCYC 3 cut(s) 913, 970, 1101
BauI CACGAG 2 cut(s) 912, 969
BbsI GAAGAC 1 cut(s) 1051
Bbv12I GWGCWC 6 cut(s) 913, 955, 970, 1101, 2423, 2485
BbvI GCAGC 9 cut(s) 178, 214, 253, 283, 970, 1213, 1363, 1522, 2285
BccI CCATC 7 cut(s) 322, 759, 763, 1619, 1985, 2053, 2321
BceAI ACGGC 4 cut(s) 243, 248, 1578, 1719
BciT130I CCWGG 2 cut(s) 1356, 1655
BclI TGATCA 5 cut(s) 655, 931, 1342, 1390, 1972
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 2 cut(s) 1291, 2271
BglI GCCNNNNNGGC 1 cut(s) 2077
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 2 cut(s) 1356, 1655
Bme18I GGWCC 6 cut(s) 1701, 1718, 1870, 2086, 2331, 2358
BmeRI GACNNNNNGTC 1 cut(s) 2264
BmeT110I CYCGRG 1 cut(s) 1680
BmgBI CACGTC 1 cut(s) 694
BmgT120I GGNCC 8 cut(s) 919, 1701, 1709, 1718, 1870, 2086, 2331, 2358
BmiI GGNNCC 9 cut(s) 251, 685, 920, 1286, 1476, 1720, 1856, 2332, 2359
BmrFI CCNGG 2 cut(s) 1356, 1655
BpiI GAAGAC 1 cut(s) 1051
BpmI CTGGAG 1 cut(s) 2165
Bpu10I CCTNAGC 1 cut(s) 254
BpuEI CTTGAG 3 cut(s) 385, 397, 939
BsaBI GATNNNNATC 1 cut(s) 1971
BsaI GGTCTC 1 cut(s) 2112
BsaJI CCNNGG 3 cut(s) 1221, 1653, 1654
BsaXI ACNNNNNCTCC 8 cut(s) 298, 328, 374, 404, 1024, 1054, 1531, 1561
Bsc4I CCNNNNNNNGG 7 cut(s) 231, 303, 349, 405, 722, 1520, 1920
Bse1I ACTGG 5 cut(s) 781, 942, 1243, 1478, 2054
Bse21I CCTNAGG 1 cut(s) 1602
Bse3DI GCAATG 1 cut(s) 1192
Bse8I GATNNNNATC 1 cut(s) 1971
BseBI CCWGG 2 cut(s) 1356, 1655
BseDI CCNNGG 3 cut(s) 1221, 1653, 1654
BseJI GATNNNNATC 1 cut(s) 1971
BseLI CCNNNNNNNGG 7 cut(s) 231, 303, 349, 405, 722, 1520, 1920
BseMI GCAATG 1 cut(s) 1192
BseMII CTCAG 9 cut(s) 245, 449, 821, 1180, 1593, 1808, 1819, 2173, 2304
BseNI ACTGG 5 cut(s) 781, 942, 1243, 1478, 2054
BseRI GAGGAG 7 cut(s) 120, 193, 319, 358, 547, 921, 2178
BseSI GKGCMC 5 cut(s) 93, 2061, 2415, 2423, 2471
BseXI GCAGC 9 cut(s) 178, 214, 253, 283, 970, 1213, 1363, 1522, 2285
BsgI GTGCAG 2 cut(s) 213, 329
Bsh1236I CGCG 2 cut(s) 247, 499
BshFI GGCC 7 cut(s) 342, 427, 921, 1002, 1600, 1711, 2219
BshNI GGYRCC 1 cut(s) 683
BsiHKAI GWGCWC 6 cut(s) 913, 955, 970, 1101, 2423, 2485
BsiHKCI CYCGRG 1 cut(s) 1680
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 4 cut(s) 991, 1037, 1704, 2371
BslI CCNNNNNNNGG 7 cut(s) 231, 303, 349, 405, 722, 1520, 1920
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 4 cut(s) 991, 1037, 1704, 2371
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 7 cut(s) 342, 427, 921, 1002, 1600, 1711, 2219
Bso31I GGTCTC 1 cut(s) 2112
BsoBI CYCGRG 1 cut(s) 1680
Bsp1407I TGTACA 2 cut(s) 1791, 2400
Bsp19I CCATGG 1 cut(s) 1221
BspANI GGCC 7 cut(s) 342, 427, 921, 1002, 1600, 1711, 2219
BspCNI CTCAG 9 cut(s) 246, 448, 820, 1179, 1594, 1809, 1818, 2172, 2305
BspFNI CGCG 2 cut(s) 247, 499
BspHI TCATGA 1 cut(s) 1969
BspLI GGNNCC 9 cut(s) 251, 685, 920, 1286, 1476, 1720, 1856, 2332, 2359
BspMAI CTGCAG 1 cut(s) 2275
BspPI GGATC 2 cut(s) 244, 257
BspT107I GGYRCC 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 2112
BsrDI GCAATG 1 cut(s) 1192
BsrGI TGTACA 2 cut(s) 1791, 2400
BsrI ACTGG 5 cut(s) 781, 942, 1243, 1478, 2054
BssECI CCNNGG 3 cut(s) 1221, 1653, 1654
BssSI CACGAG 2 cut(s) 912, 969
BssT1I CCWWGG 1 cut(s) 1221
Bst2BI CACGAG 2 cut(s) 912, 969
Bst2UI CCWGG 2 cut(s) 1356, 1655
Bst4CI ACNGT 5 cut(s) 352, 390, 683, 1264, 2520
BstAPI GCANNNNNTGC 1 cut(s) 172
BstAUI TGTACA 2 cut(s) 1791, 2400
BstC8I GCNNGC 7 cut(s) 276, 340, 344, 2120, 2221, 2411, 2471
BstDEI CTNAG 9 cut(s) 254, 435, 807, 1166, 1602, 1805, 1817, 2159, 2313
BstDSI CCRYGG 1 cut(s) 1221
BstFNI CGCG 2 cut(s) 247, 499
BstHHI GCGC 4 cut(s) 271, 280, 499, 1185
BstMWI GCNNNNNNNGC 7 cut(s) 172, 275, 277, 1036, 1410, 1993, 2077
BstNI CCWGG 2 cut(s) 1356, 1655
BstNSI RCATGY 5 cut(s) 485, 1030, 2122, 2203, 2419
BstSCI CCNGG 2 cut(s) 1354, 1653
BstSFI CTRYAG 2 cut(s) 1291, 2271
BstSLI GKGCMC 5 cut(s) 93, 2061, 2415, 2423, 2471
BstUI CGCG 2 cut(s) 247, 499
BstV1I GCAGC 9 cut(s) 178, 214, 253, 283, 970, 1213, 1363, 1522, 2285
BstV2I GAAGAC 1 cut(s) 1051
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
Bsu36I CCTNAGG 1 cut(s) 1602
BsuRI GGCC 7 cut(s) 342, 427, 921, 1002, 1600, 1711, 2219
BtgI CCRYGG 1 cut(s) 1221
BtrI CACGTC 1 cut(s) 694
BtsI GCAGTG 1 cut(s) 1855
BtsIMutI CAGTG 4 cut(s) 949, 1260, 1855, 2061
Cac8I GCNNGC 7 cut(s) 276, 340, 344, 2120, 2221, 2411, 2471
CaiI CAGNNNCTG 2 cut(s) 986, 1484
CciI TCATGA 1 cut(s) 1969
CfoI GCGC 4 cut(s) 271, 280, 499, 1185
Cfr13I GGNCC 8 cut(s) 919, 1701, 1709, 1718, 1870, 2086, 2331, 2358
CseI GACGC 2 cut(s) 623, 1418
Csp6I GTAC 5 cut(s) 432, 758, 1677, 1792, 2401
CviQI GTAC 5 cut(s) 432, 758, 1677, 1792, 2401
DdeI CTNAG 9 cut(s) 254, 435, 807, 1166, 1602, 1805, 1817, 2159, 2313
DraIII CACNNNGTG 1 cut(s) 674
DriI GACNNNNNGTC 1 cut(s) 2264
Eam1105I GACNNNNNGTC 1 cut(s) 2264
EciI GGCGGA 3 cut(s) 626, 1761, 2472
Ecl136II GAGCTC 3 cut(s) 911, 968, 1099
Eco130I CCWWGG 1 cut(s) 1221
Eco147I AGGCCT 2 cut(s) 342, 2219
Eco24I GRGCYC 3 cut(s) 913, 970, 1101
Eco31I GGTCTC 1 cut(s) 2112
Eco47I GGWCC 6 cut(s) 1701, 1718, 1870, 2086, 2331, 2358
Eco53kI GAGCTC 3 cut(s) 911, 968, 1099
Eco57I CTGAAG 1 cut(s) 1506
Eco81I CCTNAGG 1 cut(s) 1602
Eco88I CYCGRG 1 cut(s) 1680
EcoICRI GAGCTC 3 cut(s) 911, 968, 1099
EcoO109I RGGNCCY 3 cut(s) 1709, 2086, 2358
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 2 cut(s) 1354, 1653
EcoT14I CCWWGG 1 cut(s) 1221
EcoT22I ATGCAT 1 cut(s) 2124
EcoT38I GRGCYC 3 cut(s) 913, 970, 1101
ErhI CCWWGG 1 cut(s) 1221
Esp3I CGTCTC 1 cut(s) 175
FalI AAGNNNNNCTT 2 cut(s) 1201, 1233
FaqI GGGAC 4 cut(s) 991, 1037, 1704, 2371
FauI CCCGC 4 cut(s) 78, 1980, 2402, 2478
FauNDI CATATG 1 cut(s) 1888
FbaI TGATCA 5 cut(s) 655, 931, 1342, 1390, 1972
FblI GTMKAC 2 cut(s) 560, 2176
FriOI GRGCYC 3 cut(s) 913, 970, 1101
FspBI CTAG 1 cut(s) 699
FspI TGCGCA 2 cut(s) 270, 1184
GlaI GCGC 4 cut(s) 270, 279, 498, 1184
GsuI CTGGAG 1 cut(s) 2165
HaeIII GGCC 7 cut(s) 342, 427, 921, 1002, 1600, 1711, 2219
HapII CCGG 1 cut(s) 32
HgaI GACGC 2 cut(s) 623, 1418
HhaI GCGC 4 cut(s) 271, 280, 499, 1185
Hin6I GCGC 4 cut(s) 269, 278, 497, 1183
HinP1I GCGC 4 cut(s) 269, 278, 497, 1183
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HindIII AAGCTT 1 cut(s) 1402
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 6 cut(s) 561, 668, 677, 1792, 2177, 2421
Hpy8I GTNNAC 6 cut(s) 561, 668, 677, 1792, 2177, 2421
HpyAV CCTTC 6 cut(s) 992, 1227, 1274, 1700, 1722, 2040
HpyCH4III ACNGT 5 cut(s) 352, 390, 683, 1264, 2520
HpyCH4IV ACGT 3 cut(s) 673, 693, 2248
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 275, 277, 1036, 1410, 1993, 2077
HpyF3I CTNAG 9 cut(s) 254, 435, 807, 1166, 1602, 1805, 1817, 2159, 2313
HpySE526I ACGT 3 cut(s) 673, 693, 2248
HspAI GCGC 4 cut(s) 269, 278, 497, 1183
Ksp22I TGATCA 5 cut(s) 655, 931, 1342, 1390, 1972
LmnI GCTCC 6 cut(s) 908, 1096, 1114, 1507, 1915, 1978
Lsp1109I GCAGC 9 cut(s) 178, 214, 253, 283, 970, 1213, 1363, 1522, 2285
MaeI CTAG 1 cut(s) 699
MaeII ACGT 3 cut(s) 673, 693, 2248
MaeIII GTNAC 6 cut(s) 18, 203, 384, 648, 971, 2195
MfeI CAATTG 2 cut(s) 1346, 2234
MflI RGATCY 1 cut(s) 249
MlyI GAGTC 8 cut(s) 227, 359, 392, 551, 566, 757, 2172, 2203
MmeI TCCRAC 4 cut(s) 171, 1351, 1672, 2193
Mph1103I ATGCAT 1 cut(s) 2124
MseI TTAA 3 cut(s) 1274, 2022, 2151
MslI CAYNNNNRTG 4 cut(s) 96, 569, 1671, 2228
MspA1I CMGCKG 4 cut(s) 41, 227, 983, 1987
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 1356, 1655
MunI CAATTG 2 cut(s) 1346, 2234
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 2 cut(s) 1356, 1655
MvnI CGCG 2 cut(s) 247, 499
MwoI GCNNNNNNNGC 7 cut(s) 172, 275, 277, 1036, 1410, 1993, 2077
NcoI CCATGG 1 cut(s) 1221
NdeI CATATG 1 cut(s) 1888
NlaIV GGNNCC 9 cut(s) 251, 685, 920, 1286, 1476, 1720, 1856, 2332, 2359
NmuCI GTSAC 5 cut(s) 203, 384, 648, 971, 2195
NsbI TGCGCA 2 cut(s) 270, 1184
NsiI ATGCAT 1 cut(s) 2124
NspI RCATGY 5 cut(s) 485, 1030, 2122, 2203, 2419
PaeI GCATGC 1 cut(s) 2122
PagI TCATGA 1 cut(s) 1969
PasI CCCWGGG 1 cut(s) 1654
PceI AGGCCT 2 cut(s) 342, 2219
PciI ACATGT 1 cut(s) 2415
PcsI WCGNNNNNNNCGW 1 cut(s) 910
PctI GAATGC 1 cut(s) 1152
PflMI CCANNNNNTGG 1 cut(s) 1920
PleI GAGTC 8 cut(s) 226, 359, 391, 550, 565, 756, 2172, 2202
PpsI GAGTC 8 cut(s) 226, 359, 391, 550, 565, 756, 2172, 2202
PpuMI RGGWCCY 2 cut(s) 2086, 2358
PscI ACATGT 1 cut(s) 2415
PshBI ATTAAT 1 cut(s) 2151
Psp124BI GAGCTC 3 cut(s) 913, 970, 1101
Psp1406I AACGTT 1 cut(s) 2248
Psp5II RGGWCCY 2 cut(s) 2086, 2358
Psp6I CCWGG 2 cut(s) 1354, 1653
PspGI CCWGG 2 cut(s) 1354, 1653
PspN4I GGNNCC 9 cut(s) 251, 685, 920, 1286, 1476, 1720, 1856, 2332, 2359
PspPI GGNCC 8 cut(s) 919, 1701, 1709, 1718, 1870, 2086, 2331, 2358
PspPPI RGGWCCY 2 cut(s) 2086, 2358
PstI CTGCAG 1 cut(s) 2275
PstNI CAGNNNCTG 2 cut(s) 986, 1484
PsuI RGATCY 1 cut(s) 249
PvuII CAGCTG 1 cut(s) 983
RsaI GTAC 5 cut(s) 433, 759, 1678, 1793, 2402
RsaNI GTAC 5 cut(s) 432, 758, 1677, 1792, 2401
RseI CAYNNNNRTG 4 cut(s) 96, 569, 1671, 2228
SacI GAGCTC 3 cut(s) 913, 970, 1101
SalI GTCGAC 1 cut(s) 559
SaqAI TTAA 3 cut(s) 1274, 2022, 2151
Sau96I GGNCC 8 cut(s) 919, 1701, 1709, 1718, 1870, 2086, 2331, 2358
ScaI AGTACT 1 cut(s) 433
SchI GAGTC 8 cut(s) 227, 359, 392, 551, 566, 757, 2172, 2203
ScrFI CCNGG 2 cut(s) 1356, 1655
SfcI CTRYAG 2 cut(s) 1291, 2271
SinI GGWCC 6 cut(s) 1701, 1718, 1870, 2086, 2331, 2358
SmiMI CAYNNNNRTG 4 cut(s) 96, 569, 1671, 2228
SmlI CTYRAG 3 cut(s) 376, 400, 954
SmoI CTYRAG 3 cut(s) 376, 400, 954
SphI GCATGC 1 cut(s) 2122
SseBI AGGCCT 2 cut(s) 342, 2219
SspI AATATT 2 cut(s) 450, 1306
SspMI CTAG 1 cut(s) 699
SstI GAGCTC 3 cut(s) 913, 970, 1101
StuI AGGCCT 2 cut(s) 342, 2219
StyD4I CCNGG 2 cut(s) 1354, 1653
StyI CCWWGG 1 cut(s) 1221
TaaI ACNGT 5 cut(s) 352, 390, 683, 1264, 2520
TaiI ACGT 3 cut(s) 676, 696, 2251
TaqI TCGA 9 cut(s) 356, 560, 964, 1101, 1144, 1590, 1642, 2479, 2503
TaqII GACCGA 2 cut(s) 1511, 1718
TatI WGTACW 3 cut(s) 431, 1791, 2400
TauI GCSGC 1 cut(s) 409
Tru1I TTAA 3 cut(s) 1274, 2022, 2151
Tru9I TTAA 3 cut(s) 1274, 2022, 2151
TscAI CASTG 4 cut(s) 949, 1267, 1855, 2061
TseFI GTSAC 5 cut(s) 203, 384, 648, 971, 2195
TseI GCWGC 9 cut(s) 166, 227, 266, 271, 983, 1226, 1351, 1510, 2273
Tsp45I GTSAC 5 cut(s) 203, 384, 648, 971, 2195
TspDTI ATGAA 9 cut(s) 70, 114, 162, 783, 1008, 1203, 1548, 1757, 2277
TspGWI ACGGA 3 cut(s) 20, 199, 1847
TspRI CASTG 4 cut(s) 949, 1267, 1855, 2061
Van91I CCANNNNNTGG 1 cut(s) 1920
VneI GTGCAC 1 cut(s) 2419
VpaK11BI GGWCC 6 cut(s) 1701, 1718, 1870, 2086, 2331, 2358
VspI ATTAAT 1 cut(s) 2151
XapI RAATTY 2 cut(s) 77, 1582
XceI RCATGY 5 cut(s) 485, 1030, 2122, 2203, 2419
XcmI CCANNNNNNNNNTGG 1 cut(s) 2073
XmiI GTMKAC 2 cut(s) 560, 2176
XspI CTAG 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 433
Zsp2I ATGCAT 1 cut(s) 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.