MD02G1254600.v1.1

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009713
Physical Location & Seq
Forward (+)
30624666 .. 30630552
5887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1254600.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGCATTCTCGAAAGCATGAGAATTTCAATAAGCAATCCCCAGCTTCTAGATTACATGCGGTGGGTCCCGACCCGTATAGTAATAGTGGTAGGAGGAGTAATATGCAACTCGAGGCTGCTGATCAGTCTAGTGAGAGGAGTTTAAGCCCTCGCGGATTGCCAGGGGATGTGGGTTTGAGTCAAAGGACTGATTCTATTGGAAGGAGTGACCATGGTTGGCATTTGGGTGGTAGAAGGACTGATCGGGTGAGGCGGAGATCAAGGTCAACGTCACCGCCACTGCCTTTTGGCGGCATTCAGAAAAGGTCACATTTTGATGAAGGAGTTGGGGTTATGCATAGGAATTATTCGCCAACTCCACTTGCACCTGTTCCTTTGGAGTTACAACACAGACCTGAACTTGTAGAGCCTGCAAAATATAGTGTGAATGATGATTCAAATAGTAGACGAGTTTACGGGTCTGAGAACAATGATTCTAAAATCATCAATGAGGAAATGAATGGAAGCAGATTGTCAGTTGGCGGTACACATGGGACATTGAGTCAGAAATCAATGCATGTGGAAGATGGTGCGGTAAGAGGATTAAAATCCACACTAATTCAAGATAATACTGCACTAGGAATATATTGGCCACCTCCAGACCTGCATCCTGTGATAGCTCCTGCAAAAGATAGTGAACACCTACCATCATCATCACGGAGTATGAACTTACCCCGTTTTGAGCATGGAAGGCCTCGGTATACGGATCCTGTTGCTTTGGATAGATTACCAGTGACAGAATCCTACAAAGGAGAGAAACCCATTCTTACTTCAAGGGATGGTTTGTACCCCATGGTGTCAAGTGCTCATTATACGGACTTTCGTCCTAGCTCCTCCACAGACGTAAGGAGTGAATTTCAGGATTCTTATATGGGTGGCCCACACTTACCTTCAATGGATGAATTTTCAAGTAGTAGACGGTTCATTAATGCATATAGACAAAGGCCACTAGCAGATTATCCTAGAGATCCTGGTTCTGGCAAGAGGAACCTGTCAACTTACCAGAGTTATAGTCCTAACAGAGGTGAGCATGCAGATTATTTTTACCCCAAATCAAGGGGAATGCCAGTTGATGATCGCAGATATCCATCTGATGACTTAAATAAAATCATGCCTCCACGAACACAACTTGACTATGATAGTACTCAAATGGTTTATAACCATCAAAATTTGTCAAGACCTAGTATTATGAACCCTGTTATGGATAGAATGGATGATACTGAGGAATTTTCTGGAAATTCAAGAAAGGGCATTATGCTGAATAATCCTACTTTGCAAAGGCAATCTTTATTAGACTACCCTGATTCAAGAAGAATATCAGAGACATCAAAGCATGGTGTGGAATATTCAGGTTCAGGACGCACACATGTCAGTTTAGGAAGGAGAATGTCACAAGATTATGAACTATCCCATTTCAGGGCATCACAAGATTTTCAGGTCGCACATCAGAAAGAAGATTATGGTTTTGAAAGAGATGTTAATATGAAGTATCAGGACAGGCCGTCTTCTGTGTCAAAATATGACTCAGAGATGAGTGGGCATCCTGCTGGAATGCAGATTATGAGAGAGGAGCTTGGAATATATGAACCATCAGATAGGATGCTTAAAAGAAACTATGCTACTGAAGAAGGTATGAGTACACATAATCCTAGAACTATTGGGTCTAGTAAGTGGACATCTAGAGAATTCCAAGTTTCATATGAAAGCGGTGAAGAGTGGAATGATGGAGATTTAGGCAGTTATTCATCTGCATCAGCTGGATTTGACCATGATAGATACAGTAAGGCTGAGAGGGTATATGTTGGGCACCGTCATGGTGAATATGAATATGATGATTGGTTGCCATCTCAACACTCTTTTGAACATGCACAAATGCATTCAGTCAGATTCTATAAACACGGTGATCGATATATAAAGGGTCATCGAAATTCTGGTCCATTAAGTAGGCATAAGGCGCATCACGCTGATATAAAAAGCAGTGTTCACAAACAGCACCGAGTTTGGAAAAGACATTATAATTATTTAGAAGATGTCCACGCAAGTGATGGTACTGATGTTGATCAATCAGAAAATGGGCTGAGCTCTGCACGTCCTGAGCCGTCCGAGGACTCTGAGGAGTTTATGCAAATGGTAAATGAAGCCTTTTTAACATTTTCTAAAAAGCTAAATATGAACTCGGCTGTTCGGAGAAGATACAAGGAACAAGGGAAGGCTGGTACTTTGTTCTGCATCGTATGTGGCCGAAGTTTGTCGAAGGAGTTCATGGACACTCAACGCTTGGTAAGACACGCATATATGTCTCACAGAGTTGGGCTGAGGGCACAGCACTTGGGTCTTCTAAAAGCAGTTTGTGTTCTGTTGGGGTGGAGTACTGTTGTCCCCCCTGACACAGTCACATGGGCTCCCCAGGTCTTGCCCAAGGCTGAAGCTTTGGCTCAGAAGGAAGATTTGATTCTCTGGCCTCCTGTGATTGTTGTTCACAATATTTCTATGTCAGACAACAATCCTCAAAGTTGGAAGGTTGTTTCCATGGAGGCGCTCGAGGCTTTTCTAAGAAGTAATGGTTTAATCAAGGGAAGAATCAAGATTTGCCTAGGGAAGCCTGCTGACCAAAGTGTTTTGGTGGTGAAGTTCTTGGGAACTTTTACTGGGCTTGGGGACGCAGAGAGGATTCAGAAGCATTTTGCAGAGCATCAGCGTGGCAGAGTGGATTTTGAGCGAGCGACGTCCTCAAATGGAAAAATTGTGGAAGCAGCAATGCAGGGAGACAACGCAGAGGAGCGATTTCTATATGGGTACATGGGCATTGTAGAGGACTTGGATAAGGTGGATTTCCATACCAGGAGCTGGACTGTGATAAAGAGCAAGAAGGAGATTCAGGACCTGGCCAATGCTCCCGTCAAACCTGATGAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

986

Amino Acids

111.94

Weight (kDa)

8.28

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 828 - 954 2e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1197, 2055
AatII GACGTC 1 cut(s) 2768
Acc36I ACCTGC 1 cut(s) 651
AccB1I GGYRCC 1 cut(s) 1845
AccB7I CCANNNNNTGG 1 cut(s) 2886
AccI GTMKAC 3 cut(s) 445, 740, 955
AccII CGCG 1 cut(s) 153
AciI CCGC 8 cut(s) 59, 153, 253, 275, 291, 522, 572, 1746
AclWI GGATC 3 cut(s) 740, 753, 1001
AcoI YGGCCR 3 cut(s) 629, 2278, 2925
AcsI RAATTY 8 cut(s) 22, 893, 941, 1207, 1265, 1276, 1724, 1966
AcuI CTGAAG 2 cut(s) 1683, 2484
AcyI GRCGYC 1 cut(s) 2765
AfaI GTAC 8 cut(s) 526, 827, 1183, 1678, 2089, 2257, 2410, 2837
AfiI CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1095, 1240, 1455, 1456, 2886
AflIII ACRYGT 1 cut(s) 1405
AhdI GACNNNNNGTC 2 cut(s) 540, 1540
AjiI CACGTC 1 cut(s) 2129
AjnI CCWGG 5 cut(s) 160, 1009, 2445, 2879, 2922
AleI CACNNNNGTG 1 cut(s) 2079
AluBI AGCT 9 cut(s) 44, 659, 870, 1612, 1796, 2121, 2203, 2468, 2886
AluI AGCT 9 cut(s) 44, 659, 870, 1612, 1796, 2121, 2203, 2468, 2886
Alw21I GWGCWC 2 cut(s) 847, 2123
Alw26I GTCTC 3 cut(s) 1355, 2343, 2799
AlwI GGATC 3 cut(s) 740, 753, 1001
AlwNI CAGNNNCTG 2 cut(s) 2886, 2923
Ama87I CYCGRG 2 cut(s) 110, 2579
AoxI GGCC 8 cut(s) 629, 731, 916, 983, 1538, 2278, 2498, 2925
ApeKI GCWGC 2 cut(s) 116, 2792
ApoI RAATTY 8 cut(s) 22, 893, 941, 1207, 1265, 1276, 1724, 1966
ArsI GACNNNNNNTTYG 2 cut(s) 1957, 1989
AseI ATTAAT 1 cut(s) 966
Asp700I GAANNNNTTC 1 cut(s) 2297
AspA2I CCTAGG 1 cut(s) 2632
AspLEI GCGC 2 cut(s) 1996, 2578
AspS9I GGNCC 4 cut(s) 65, 917, 1973, 2920
AsuHPI GGTGA 7 cut(s) 259, 264, 1076, 1760, 1868, 1952, 2677
AvaI CYCGRG 2 cut(s) 110, 2579
AvaII GGWCC 3 cut(s) 65, 1973, 2920
AvrII CCTAGG 1 cut(s) 2632
BaeGI GKGCMC 2 cut(s) 1848, 2362
BaeI ACNNNNGTAYC 2 cut(s) 2079, 2112
BalI TGGCCA 2 cut(s) 631, 2927
BamHI GGATCC 1 cut(s) 745
BanI GGYRCC 1 cut(s) 1845
BanII GRGCYC 2 cut(s) 2123, 2443
BbsI GAAGAC 2 cut(s) 1536, 2366
Bbv12I GWGCWC 2 cut(s) 847, 2123
BbvCI CCTCAGC 1 cut(s) 2354
BbvI GCAGC 2 cut(s) 103, 2804
BccI CCATC 9 cut(s) 560, 694, 812, 1135, 1209, 1636, 1757, 1891, 2078
BceAI ACGGC 2 cut(s) 1525, 2122
BciT130I CCWGG 5 cut(s) 162, 1011, 2447, 2881, 2924
BclI TGATCA 2 cut(s) 121, 2098
BcoDI GTCTC 3 cut(s) 1355, 2343, 2799
BfoI RGCGCY 1 cut(s) 2579
BfuAI ACCTGC 1 cut(s) 651
BisI GCNGC 3 cut(s) 117, 292, 2793
BlnI CCTAGG 1 cut(s) 2632
BlpI GCTNAGC 1 cut(s) 2117
BlsI GCNGC 3 cut(s) 118, 293, 2794
BmcAI AGTACT 2 cut(s) 1183, 2410
Bme1390I CCNGG 5 cut(s) 162, 1011, 2447, 2881, 2924
Bme18I GGWCC 3 cut(s) 65, 1973, 2920
BmeRI GACNNNNNGTC 2 cut(s) 540, 1540
BmeT110I CYCGRG 2 cut(s) 110, 2579
BmgBI CACGTC 1 cut(s) 2129
BmgT120I GGNCC 4 cut(s) 65, 917, 1973, 2920
BmiI GGNNCC 6 cut(s) 66, 67, 747, 1028, 1847, 2442
BmrFI CCNGG 5 cut(s) 162, 1011, 2447, 2881, 2924
BmrI ACTGGG 1 cut(s) 2697
BmsI GCATC 8 cut(s) 655, 1469, 1588, 1629, 1799, 2005, 2277, 2740
BmuI ACTGGG 1 cut(s) 2697
BoxI GACNNNNGTC 1 cut(s) 861
BpiI GAAGAC 2 cut(s) 1536, 2366
BpmI CTGGAG 1 cut(s) 621
Bpu10I CCTNAGC 2 cut(s) 2133, 2354
Bpu1102I GCTNAGC 1 cut(s) 2117
Bsa29I ATCGAT 1 cut(s) 1945
BsaBI GATNNNNATC 3 cut(s) 586, 1126, 2097
BsaHI GRCGYC 1 cut(s) 2765
BsaJI CCNNGG 9 cut(s) 161, 211, 734, 831, 2142, 2445, 2457, 2568, 2632
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 2425, 2455
Bsc4I CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1095, 1240, 1455, 1456, 2886
Bse1I ACTGG 3 cut(s) 770, 1106, 2692
Bse3DI GCAATG 1 cut(s) 2802
Bse8I GATNNNNATC 3 cut(s) 586, 1126, 2097
BseBI CCWGG 5 cut(s) 162, 1011, 2447, 2881, 2924
BseCI ATCGAT 1 cut(s) 1945
BseDI CCNNGG 9 cut(s) 161, 211, 734, 831, 2142, 2445, 2457, 2568, 2632
BseGI GGATG 7 cut(s) 172, 646, 823, 943, 1258, 1579, 1644
BseJI GATNNNNATC 3 cut(s) 586, 1126, 2097
BseLI CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1095, 1240, 1455, 1456, 2886
BseMI GCAATG 1 cut(s) 2802
BseMII CTCAG 9 cut(s) 453, 1251, 1578, 1818, 2108, 2124, 2142, 2345, 2489
BseNI ACTGG 3 cut(s) 770, 1106, 2692
BseRI GAGGAG 6 cut(s) 109, 151, 862, 1622, 2168, 2831
BseSI GKGCMC 2 cut(s) 1848, 2362
BseXI GCAGC 2 cut(s) 103, 2804
BseYI CCCAGC 1 cut(s) 40
BsgI GTGCAG 2 cut(s) 597, 2109
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 8 cut(s) 631, 733, 918, 985, 1540, 2280, 2500, 2927
BshNI GGYRCC 1 cut(s) 1845
BshVI ATCGAT 1 cut(s) 1945
BsiHKAI GWGCWC 2 cut(s) 847, 2123
BsiHKCI CYCGRG 2 cut(s) 110, 2579
BslFI GGGAC 4 cut(s) 51, 547, 2402, 2711
BslI CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1095, 1240, 1455, 1456, 2886
BsmAI GTCTC 3 cut(s) 1355, 2343, 2799
BsmFI GGGAC 4 cut(s) 51, 547, 2402, 2711
BsmI GAATGC 5 cut(s) 4, 294, 1107, 1596, 1915
BsnI GGCC 8 cut(s) 631, 733, 918, 985, 1540, 2280, 2500, 2927
BsoBI CYCGRG 2 cut(s) 110, 2579
Bsp1286I GDGCHC 5 cut(s) 847, 1848, 2123, 2362, 2443
Bsp143I GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
Bsp1720I GCTNAGC 1 cut(s) 2117
Bsp19I CCATGG 3 cut(s) 211, 831, 2568
BspACI CCGC 8 cut(s) 59, 153, 253, 275, 291, 522, 572, 1746
BspANI GGCC 8 cut(s) 631, 733, 918, 985, 1540, 2280, 2500, 2927
BspCNI CTCAG 9 cut(s) 454, 1252, 1577, 1819, 2109, 2125, 2143, 2346, 2488
BspDI ATCGAT 1 cut(s) 1945
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 6 cut(s) 66, 67, 747, 1028, 1847, 2442
BspMI ACCTGC 1 cut(s) 651
BspPI GGATC 3 cut(s) 740, 753, 1001
BspT107I GGYRCC 1 cut(s) 1845
BsrDI GCAATG 1 cut(s) 2802
BsrI ACTGG 3 cut(s) 770, 1106, 2692
BssECI CCNNGG 9 cut(s) 161, 211, 734, 831, 2142, 2445, 2457, 2568, 2632
BssMI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
BssNAI GTATAC 1 cut(s) 741
BssNI GRCGYC 1 cut(s) 2765
BssT1I CCWWGG 5 cut(s) 211, 831, 2457, 2568, 2632
Bst1107I GTATAC 1 cut(s) 741
Bst2UI CCWGG 5 cut(s) 162, 1011, 2447, 2881, 2924
Bst4CI ACNGT 7 cut(s) 960, 1820, 1850, 1940, 2413, 2431, 2893
Bst6I CTCTTC 1 cut(s) 1746
BstACI GRCGYC 1 cut(s) 2765
BstC8I GCNNGC 4 cut(s) 411, 1071, 2643, 2760
BstDSI CCRYGG 3 cut(s) 211, 831, 2568
BstENI CCTNNNNNAGG 1 cut(s) 1059
BstF5I GGATG 7 cut(s) 172, 646, 823, 943, 1258, 1579, 1644
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 2579
BstHHI GCGC 2 cut(s) 1996, 2578
BstKTI GATC 8 cut(s) 124, 244, 260, 748, 1009, 1117, 1945, 2101
BstMAI GTCTC 3 cut(s) 1355, 2343, 2799
BstMBI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
BstMWI GCNNNNNNNGC 4 cut(s) 730, 1993, 2000, 2582
BstNI CCWGG 5 cut(s) 162, 1011, 2447, 2881, 2924
BstNSI RCATGY 5 cut(s) 59, 560, 1073, 1409, 1907
BstPAI GACNNNNGTC 1 cut(s) 861
BstSCI CCNGG 5 cut(s) 160, 1009, 2445, 2879, 2922
BstSLI GKGCMC 2 cut(s) 1848, 2362
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 103, 2804
BstV2I GAAGAC 2 cut(s) 1536, 2366
BstX2I RGATCY 2 cut(s) 745, 1006
BstYI RGATCY 2 cut(s) 745, 1006
BstZ17I GTATAC 1 cut(s) 741
Bsu15I ATCGAT 1 cut(s) 1945
BsuRI GGCC 8 cut(s) 631, 733, 918, 985, 1540, 2280, 2500, 2927
BsuTUI ATCGAT 1 cut(s) 1945
BtgI CCRYGG 3 cut(s) 211, 831, 2568
BtrI CACGTC 1 cut(s) 2129
BtsCI GGATG 7 cut(s) 172, 646, 823, 943, 1258, 1579, 1644
BtsI GCAGTG 2 cut(s) 278, 2023
BtsIMutI CAGTG 3 cut(s) 278, 777, 2023
BveI ACCTGC 1 cut(s) 651
Cac8I GCNNGC 4 cut(s) 411, 1071, 2643, 2760
CaiI CAGNNNCTG 2 cut(s) 2886, 2923
CfoI GCGC 2 cut(s) 1996, 2578
Cfr13I GGNCC 4 cut(s) 65, 917, 1973, 2920
ClaI ATCGAT 1 cut(s) 1945
CseI GACGC 2 cut(s) 1407, 2708
Csp6I GTAC 8 cut(s) 525, 826, 1182, 1677, 2088, 2256, 2409, 2836
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviQI GTAC 8 cut(s) 525, 826, 1182, 1677, 2088, 2256, 2409, 2836
DpnI GATC 8 cut(s) 123, 243, 259, 747, 1008, 1116, 1944, 2100
DpnII GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
DriI GACNNNNNGTC 2 cut(s) 540, 1540
EaeI YGGCCR 3 cut(s) 629, 2278, 2925
Eam1104I CTCTTC 1 cut(s) 1746
Eam1105I GACNNNNNGTC 2 cut(s) 540, 1540
EarI CTCTTC 1 cut(s) 1746
EciI GGCGGA 1 cut(s) 268
Ecl136II GAGCTC 1 cut(s) 2121
Eco130I CCWWGG 5 cut(s) 211, 831, 2457, 2568, 2632
Eco147I AGGCCT 1 cut(s) 733
Eco24I GRGCYC 2 cut(s) 2123, 2443
Eco32I GATATC 1 cut(s) 1124
Eco47I GGWCC 3 cut(s) 65, 1973, 2920
Eco53kI GAGCTC 1 cut(s) 2121
Eco57I CTGAAG 2 cut(s) 1683, 2484
Eco88I CYCGRG 2 cut(s) 110, 2579
EcoICRI GAGCTC 1 cut(s) 2121
EcoNI CCTNNNNNAGG 1 cut(s) 1059
EcoO109I RGGNCCY 2 cut(s) 65, 2920
EcoRI GAATTC 1 cut(s) 1724
EcoRII CCWGG 5 cut(s) 160, 1009, 2445, 2879, 2922
EcoRV GATATC 1 cut(s) 1124
EcoT14I CCWWGG 5 cut(s) 211, 831, 2457, 2568, 2632
EcoT22I ATGCAT 5 cut(s) 6, 339, 558, 973, 1917
EcoT38I GRGCYC 2 cut(s) 2123, 2443
ErhI CCWWGG 5 cut(s) 211, 831, 2457, 2568, 2632
FalI AAGNNNNNCTT 2 cut(s) 1309, 1341
FaqI GGGAC 4 cut(s) 51, 547, 2402, 2711
FauNDI CATATG 1 cut(s) 1738
FbaI TGATCA 2 cut(s) 121, 2098
FblI GTMKAC 3 cut(s) 445, 740, 955
Fnu4HI GCNGC 3 cut(s) 117, 292, 2793
FokI GGATG 7 cut(s) 179, 633, 830, 950, 1265, 1566, 1651
FriOI GRGCYC 2 cut(s) 2123, 2443
Fsp4HI GCNGC 3 cut(s) 117, 292, 2793
GlaI GCGC 2 cut(s) 1995, 2577
GluI GCNGC 3 cut(s) 117, 292, 2793
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 621
HaeII RGCGCY 1 cut(s) 2579
HaeIII GGCC 8 cut(s) 631, 733, 918, 985, 1540, 2280, 2500, 2927
HgaI GACGC 2 cut(s) 1407, 2708
HhaI GCGC 2 cut(s) 1996, 2578
Hin1I GRCGYC 1 cut(s) 2765
Hin6I GCGC 2 cut(s) 1994, 2576
HinP1I GCGC 2 cut(s) 1994, 2576
HincII GTYRAC 2 cut(s) 267, 1035
HindII GTYRAC 2 cut(s) 267, 1035
HindIII AAGCTT 1 cut(s) 2466
HphI GGTGA 7 cut(s) 259, 264, 1076, 1760, 1868, 1952, 2677
Hpy99I CGWCG 1 cut(s) 2767
HpyCH4III ACNGT 7 cut(s) 960, 1820, 1850, 1940, 2413, 2431, 2893
HpyCH4IV ACGT 4 cut(s) 269, 882, 2128, 2765
HpyF10VI GCNNNNNNNGC 4 cut(s) 730, 1993, 2000, 2582
HpySE526I ACGT 4 cut(s) 269, 882, 2128, 2765
Hsp92I GRCGYC 1 cut(s) 2765
HspAI GCGC 2 cut(s) 1994, 2576
KflI GGGWCCC 1 cut(s) 65
Ksp22I TGATCA 2 cut(s) 121, 2098
Kzo9I GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
LmnI GCTCC 7 cut(s) 664, 875, 1609, 2446, 2818, 2883, 2938
Lsp1109I GCAGC 2 cut(s) 103, 2804
LweI GCATC 8 cut(s) 655, 1469, 1588, 1629, 1799, 2005, 2277, 2740
MaeII ACGT 4 cut(s) 269, 882, 2128, 2765
MaeIII GTNAC 7 cut(s) 206, 270, 306, 381, 772, 1428, 2431
MalI GATC 8 cut(s) 123, 243, 259, 747, 1008, 1116, 1944, 2100
MboI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
MflI RGATCY 2 cut(s) 745, 1006
MhlI GDGCHC 5 cut(s) 847, 1848, 2123, 2362, 2443
MlsI TGGCCA 2 cut(s) 631, 2927
MluNI TGGCCA 2 cut(s) 631, 2927
MlyI GAGTC 4 cut(s) 187, 550, 1556, 2141
MmeI TCCRAC 1 cut(s) 2534
Mox20I TGGCCA 2 cut(s) 631, 2927
Mph1103I ATGCAT 5 cut(s) 6, 339, 558, 973, 1917
MroXI GAANNNNTTC 1 cut(s) 2297
MscI TGGCCA 2 cut(s) 631, 2927
MseI TTAA 9 cut(s) 143, 584, 966, 1139, 1518, 1644, 1979, 2186, 2606
MslI CAYNNNNRTG 5 cut(s) 225, 315, 1851, 2079, 2736
Msp20I TGGCCA 2 cut(s) 631, 2927
MspA1I CMGCKG 1 cut(s) 1796
MspR9I CCNGG 5 cut(s) 162, 1011, 2447, 2881, 2924
Mva1269I GAATGC 5 cut(s) 4, 294, 1107, 1596, 1915
MvaI CCWGG 5 cut(s) 162, 1011, 2447, 2881, 2924
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 4 cut(s) 730, 1993, 2000, 2582
NcoI CCATGG 3 cut(s) 211, 831, 2568
NdeI CATATG 1 cut(s) 1738
NdeII GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
NlaIV GGNNCC 6 cut(s) 66, 67, 747, 1028, 1847, 2442
NmeAIII GCCGAG 1 cut(s) 2195
NmuCI GTSAC 6 cut(s) 206, 270, 306, 772, 1428, 2431
NsiI ATGCAT 5 cut(s) 6, 339, 558, 973, 1917
NspI RCATGY 5 cut(s) 59, 560, 1073, 1409, 1907
OliI CACNNNNGTG 1 cut(s) 2079
PaeI GCATGC 1 cut(s) 1073
PaeR7I CTCGAG 2 cut(s) 110, 2579
PceI AGGCCT 1 cut(s) 733
PciI ACATGT 1 cut(s) 1405
PctI GAATGC 5 cut(s) 4, 294, 1107, 1596, 1915
PdmI GAANNNNTTC 1 cut(s) 2297
PflFI GACNNNGTC 1 cut(s) 2429
PflMI CCANNNNNTGG 1 cut(s) 2886
PkrI GCNGC 3 cut(s) 118, 293, 2794
PleI GAGTC 4 cut(s) 186, 549, 1556, 2141
PpsI GAGTC 4 cut(s) 186, 549, 1556, 2141
PpuMI RGGWCCY 2 cut(s) 65, 2920
PscI ACATGT 1 cut(s) 1405
PshAI GACNNNNGTC 1 cut(s) 861
PshBI ATTAAT 1 cut(s) 966
PsiI TTATAA 2 cut(s) 1197, 2055
Psp124BI GAGCTC 1 cut(s) 2123
Psp5II RGGWCCY 2 cut(s) 65, 2920
Psp6I CCWGG 5 cut(s) 160, 1009, 2445, 2879, 2922
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 5 cut(s) 160, 1009, 2445, 2879, 2922
PspN4I GGNNCC 6 cut(s) 66, 67, 747, 1028, 1847, 2442
PspPI GGNCC 4 cut(s) 65, 917, 1973, 2920
PspPPI RGGWCCY 2 cut(s) 65, 2920
PspXI VCTCGAGB 2 cut(s) 110, 2579
PstNI CAGNNNCTG 2 cut(s) 2886, 2923
PsuI RGATCY 2 cut(s) 745, 1006
PsyI GACNNNGTC 1 cut(s) 2429
PvuII CAGCTG 1 cut(s) 1796
RsaI GTAC 8 cut(s) 526, 827, 1183, 1678, 2089, 2257, 2410, 2837
RsaNI GTAC 8 cut(s) 525, 826, 1182, 1677, 2088, 2256, 2409, 2836
RseI CAYNNNNRTG 5 cut(s) 225, 315, 1851, 2079, 2736
SacI GAGCTC 1 cut(s) 2123
SaqAI TTAA 9 cut(s) 143, 584, 966, 1139, 1518, 1644, 1979, 2186, 2606
SatI GCNGC 3 cut(s) 117, 292, 2793
Sau3AI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1942, 2098
Sau96I GGNCC 4 cut(s) 65, 917, 1973, 2920
ScaI AGTACT 2 cut(s) 1183, 2410
SchI GAGTC 4 cut(s) 187, 550, 1556, 2141
ScrFI CCNGG 5 cut(s) 162, 1011, 2447, 2881, 2924
SduI GDGCHC 5 cut(s) 847, 1848, 2123, 2362, 2443
SfaNI GCATC 8 cut(s) 655, 1469, 1588, 1629, 1799, 2005, 2277, 2740
Sfr274I CTCGAG 2 cut(s) 110, 2579
SinI GGWCC 3 cut(s) 65, 1973, 2920
SlaI CTCGAG 2 cut(s) 110, 2579
SmiMI CAYNNNNRTG 5 cut(s) 225, 315, 1851, 2079, 2736
SmlI CTYRAG 2 cut(s) 110, 2579
SmoI CTYRAG 2 cut(s) 110, 2579
SphI GCATGC 1 cut(s) 1073
SseBI AGGCCT 1 cut(s) 733
SsiI CCGC 8 cut(s) 59, 153, 253, 275, 291, 522, 572, 1746
SspI AATATT 2 cut(s) 1385, 2524
SstI GAGCTC 1 cut(s) 2123
StuI AGGCCT 1 cut(s) 733
StyD4I CCNGG 5 cut(s) 160, 1009, 2445, 2879, 2922
StyI CCWWGG 5 cut(s) 211, 831, 2457, 2568, 2632
TaaI ACNGT 7 cut(s) 960, 1820, 1850, 1940, 2413, 2431, 2893
TaiI ACGT 4 cut(s) 272, 885, 2131, 2768
TaqI TCGA 6 cut(s) 10, 111, 1945, 1963, 2291, 2580
TatI WGTACW 3 cut(s) 1181, 1676, 2408
TauI GCSGC 1 cut(s) 294
Tru1I TTAA 9 cut(s) 143, 584, 966, 1139, 1518, 1644, 1979, 2186, 2606
Tru9I TTAA 9 cut(s) 143, 584, 966, 1139, 1518, 1644, 1979, 2186, 2606
TscAI CASTG 3 cut(s) 285, 777, 2023
TseFI GTSAC 6 cut(s) 206, 270, 306, 772, 1428, 2431
TseI GCWGC 2 cut(s) 116, 2792
Tsp45I GTSAC 6 cut(s) 206, 270, 306, 772, 1428, 2431
TspGWI ACGGA 3 cut(s) 712, 758, 869
TspRI CASTG 3 cut(s) 285, 777, 2023
Tth111I GACNNNGTC 1 cut(s) 2429
Van91I CCANNNNNTGG 1 cut(s) 2886
VpaK11BI GGWCC 3 cut(s) 65, 1973, 2920
VspI ATTAAT 1 cut(s) 966
XagI CCTNNNNNAGG 1 cut(s) 1059
XapI RAATTY 8 cut(s) 22, 893, 941, 1207, 1265, 1276, 1724, 1966
XbaI TCTAGA 2 cut(s) 47, 1718
XceI RCATGY 5 cut(s) 59, 560, 1073, 1409, 1907
XcmI CCANNNNNNNNNTGG 1 cut(s) 2081
XhoI CTCGAG 2 cut(s) 110, 2579
XmaJI CCTAGG 1 cut(s) 2632
XmiI GTMKAC 3 cut(s) 445, 740, 955
XmnI GAANNNNTTC 1 cut(s) 2297
ZraI GACGTC 1 cut(s) 2766
ZrmI AGTACT 2 cut(s) 1183, 2410
Zsp2I ATGCAT 5 cut(s) 6, 339, 558, 973, 1917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.