Rroxscaffold_4G00317840

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009713
Physical Location & Seq
Forward (+)
43399318 .. 43405154
5837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317840.1

Sequence Viewer

Length: 3024 bp
ATGCAACCTCGGAGGCATGAGAGTTTCAATAGACAGCAGCCTCCGGCTTCTAAATTACGTCCCACTTACCGATTCGAATCCGGTGCCAACAACCCTTATAGTACTGGTAGTAGGAGGAGACCTGAGGAGGTACATCGGACTATTAGGAGGAGTTTGAGCCCGCAACAAGATCATCTGGGTGTCAGTCAAAGGAATGTTGCTTCTATTCAGAGGGAGTATGGCTGGCATTTGGGCGGCGGTGGTGGCGGTAGGACTGATGGCGTCCGCCGCAGGTCAACATCCGGGTCACCCCGACTGCTTTTTGGTGAAATGCGAAAAATGTCACAATTTGAGGAGGAGGAGGAGGAGGAGGATGGGGATTTGCGGAGGAGTTATTCACCACCACCACAACCACTGGTACCTGTTCCGGTATCTGTTCCTTTGGAGTTAAAACACAGACGTGATAGCATTGAGGCTGGAAATTATAGTGTCAATGATGATTTGAATAGTAGGAGAGTTTATGGCTCCGAGCATAATGATTTTGGAATCAGTAAAGAAGATTTGAATGAAAGCAGATTGACAGCTGGTGGTAAACATGGGACATTGGGTCAAAAACCAATCCATATGGAAGATAGTGCTGTAAGAGGATCCAAAACCATGTTTATCAAAGGTGACACTGTGCATGAAGCATATCATTCTTCTCCAAGCCTTGGTACTGTTATAACTCAAGGAGCAAGGGGTGGCCACCTACCATCATCAACGCGCAGCATGAGTATACGTCATTCTGACCAAGAAAGGCTTCAGTATGCGGACCCTATATCATTGGATAGATTACCAGTGACAGAAGCCTACAAAGAAGGAAATAAACCCGTTTTTCCTACAAGAGATGGCTTGTATTCCATGATGTCTGGATCTCATTCCAAGAATTATTTTGCTAGCTCCTCAACCAGGAATGAATTCCAAGATTCCTACCAGCATGAGCAGCATATGCCTTCTTTGGAAGAATTTTCAAGAAGTAGTAGAAAACTTAAAGATTCTGTGAGCATTAATGCATATAGGGAAAGGCCGGTAGTAGAGTTTTCTCGAGACCCTGATTCTGGGCAGAAGAATCTGACATTTTACCAGAGAGATAGTCCTACTAGAGTTGAGTATGATGATGATTATTATTTCTACCCAAACTCAAGGGGAATGATATTTGACGATCGTGAGCTTCCATCTGATCACTTACACAAAGTGATGCATCCACGAGTACCACTTGACTATGACCATTCTCTGATGGGTTATGATCATAGAACTGTGTCAAGATCTAGTACCATGCTCCCTGTTGTGGAAAGAATTGATAATACTGAGGATTATTCTGGAAATTCAAGGAAGCGCATTATGCTGAATAATTCTAGTTTAGAAAGGCATACACTATCAGACTACCCTGATATAAGCAGAATATCAAGTGCATCGAAGCAGAGTGGAGAATATATGGGTTCTGGACGCATGCATGACAAGTTTGGAAGGAGAATTTCACAAGATTATGAAACATCGCATTTGGGTGCAGCGCAAGTTTGTCAAATCTCACATCTGAAAGAAGATTATGGTTATGAAAGAGATGGAAACCTGAAGTTTCAGGACAGACAGTGTCCTGTGTCTAAATACGACCCAGAAATGCATAGAAATACTATTAGAATGCAGAGTATGAGAGACAAGCTTGGGGTATATGAACCATCAGATAGGGTGGTGAAAAGAAAATATGTTAATGAAGAGCATGGAAGTGTCCAGAATCCTAGAGAAATTATGTCAGGTAAATGGAATACCTCTAGAGAGTTTCAAGATTTGTATGATAGTGGTGAAGAGTGGAATGGTGAAGATGTAGGATCTATGTATACATCTAGATCAGCAAGATTGGGCCATAATGAACACTATAAGGCCCAGAGGAAATATGCTGGAATAGACCAGTATGATGAATTTGCATCTGATGATCGGCTGCCGTCACAAGACTCTTTGGCAGATGCACAAAGGCACTCAGTTAGATATTATAAACATAGTGATCGGTATGCCAAGGGTCACCAAAACTATGGTTCATTAAGTAGGCATAGGTCACAGCAAGTTGACATAAAAAGTGGCTTTCACAAACAGCACAAAGTTTGGAAGAGAAATGATAATTATCTAGAGGATGTCCACAGAGGTGATGATAATGATGCTGATACATCAGAAAATGGGTTGAGTTCTGCAGCTCCTGAGCCATCAGAGGATTCTGAGGAGTTTATGCAATTGGTACAAGAAGCCTTCCTAACATACTCTAAACAATTAAATATGAACCCTGGTGTTCGGAGAAGATACATGGAACAAGGAAAGGCCGGGACTTTATTCTGCATCGTATGTGGCCGAAGCTTCTCGAAGGAGTTCATGGACACCCAACGATTGGTAACACATGCATTTATGTCTAAAAAAGCTGGATTGAGGACACAACACTTGGGACTTCTCAAAGCAGTTTGTGCTCTGTTGGGGTGGAGTACTGTTATCCCCACTGATATAGTACTATGGGTTCCCCAGGTCTTGCCCAAGGCAGAAGCTTTGGCTCAGAAGGAAGATTTGATTCTTTGGCCTCCAGTCATTATTATCCACAATATTTCTATGTTAGACAACAATCCTGAAAATTGGAAGGTTGTAAGCATTGAAGCCCTTGAGGCTTTTCTAAGAGGTAAAGGTTTAATTAGGGGAAGAATCAAGATGTGCCTGGGGAAGCCTGCTGATCAAAGTGTTGTGTTGGTGAAGTTCTTGGGAACTTTCACTGGGCTTGGGAATGCAGAGAGGATTCAGAAGTATTTTGCAGAGCAGAAACGTGGCAGAGCAGACTTTGTGCAAGCAACGTCCAACAACGGAAAAATTGTGGAAGCAGGAATGCAGGGAGACAGTGTGGAGGAGCGATTTTTACATGGATATATGGGGATTGCGGAGGACTTAGATAAAGTAGATTTTAATACCAGGAACTGGAGTTTGATAAAGAGCAAGAAAGAGATTCAGGACCTGGCCAATGCTCCTGTTAAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

1007

Amino Acids

115.08

Weight (kDa)

7.99

Isoelectric Point (pI)

60.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 853 - 979 1.6e-17 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 701, 2007
Acc36I ACCTGC 1 cut(s) 261
Acc65I GGTACC 1 cut(s) 397
AccB1I GGYRCC 2 cut(s) 83, 397
AccB7I CCANNNNNTGG 3 cut(s) 689, 2392, 2961
AccI GTMKAC 2 cut(s) 754, 1853
AccII CGCG 1 cut(s) 742
AciI CCGC 9 cut(s) 161, 234, 237, 246, 265, 268, 364, 788, 2924
AclWI GGATC 4 cut(s) 621, 634, 898, 1852
AcoI YGGCCR 3 cut(s) 721, 2353, 3000
AcsI RAATTY 5 cut(s) 935, 983, 1342, 1491, 1934
AcuI CTGAAG 2 cut(s) 764, 1610
AcyI GRCGYC 1 cut(s) 261
AdeI CACNNNGTG 1 cut(s) 1213
AfaI GTAC 9 cut(s) 103, 132, 399, 694, 1230, 1291, 2247, 2485, 2508
AfiI CCNNNNNNNGG 6 cut(s) 689, 927, 1076, 1306, 2392, 2961
AgsI TTSAA 7 cut(s) 28, 484, 544, 990, 1347, 1799, 2648
AhdI GACNNNNNGTC 1 cut(s) 585
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 6 cut(s) 926, 2290, 2520, 2706, 2954, 2997
AjuI GAANNNNNNNTTGG 2 cut(s) 762, 794
AleI CACNNNNGTG 2 cut(s) 438, 2154
AluBI AGCT 8 cut(s) 563, 918, 1189, 1678, 2204, 2361, 2423, 2543
AluI AGCT 8 cut(s) 563, 918, 1189, 1678, 2204, 2361, 2423, 2543
Alw21I GWGCWC 1 cut(s) 2470
Alw26I GTCTC 4 cut(s) 112, 1059, 1665, 2874
AlwI GGATC 4 cut(s) 621, 634, 898, 1852
AlwNI CAGNNNCTG 3 cut(s) 2207, 2961, 2998
Ama87I CYCGRG 1 cut(s) 1062
AoxI GGCC 8 cut(s) 721, 1043, 1876, 1896, 2325, 2353, 2573, 3000
ApeKI GCWGC 6 cut(s) 37, 744, 961, 1526, 1954, 2201
ApoI RAATTY 5 cut(s) 935, 983, 1342, 1491, 1934
AseI ATTAAT 1 cut(s) 1026
Asp700I GAANNNNTTC 3 cut(s) 777, 935, 2372
Asp718I GGTACC 1 cut(s) 397
AspLEI GCGC 3 cut(s) 744, 1356, 1531
AspS9I GGNCC 4 cut(s) 790, 1876, 1897, 2995
AsuC2I CCSGG 2 cut(s) 283, 2329
AsuII TTCGAA 1 cut(s) 75
AsuNHI GCTAGC 1 cut(s) 914
AvaI CYCGRG 1 cut(s) 1062
AvaII GGWCC 2 cut(s) 790, 2995
AxyI CCTNAGG 1 cut(s) 123
BalI TGGCCA 2 cut(s) 723, 3002
BamHI GGATCC 1 cut(s) 626
BanI GGYRCC 2 cut(s) 83, 397
BanII GRGCYC 1 cut(s) 161
BauI CACGAG 1 cut(s) 1224
Bbv12I GWGCWC 1 cut(s) 2470
BbvI GCAGC 6 cut(s) 49, 756, 973, 1538, 1941, 2213
BccI CCATC 9 cut(s) 251, 347, 739, 860, 1201, 1249, 1574, 1702, 2221
BceAI ACGGC 1 cut(s) 1942
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 6 cut(s) 928, 2292, 2522, 2708, 2956, 2999
BclI TGATCA 3 cut(s) 1198, 1264, 2722
BcnI CCSGG 2 cut(s) 283, 2329
BcoDI GTCTC 4 cut(s) 112, 1059, 1665, 2874
BfaI CTAG 8 cut(s) 915, 1119, 1287, 1374, 1755, 1788, 1860, 2138
BfmI CTRYAG 1 cut(s) 2199
BfuAI ACCTGC 1 cut(s) 261
BglI GCCNNNNNGGC 1 cut(s) 2657
BglII AGATCT 1 cut(s) 1283
BisI GCNGC 8 cut(s) 38, 235, 268, 745, 962, 1527, 1955, 2202
BlsI GCNGC 8 cut(s) 39, 236, 269, 746, 963, 1528, 1956, 2203
BmcAI AGTACT 3 cut(s) 103, 2485, 2508
Bme1390I CCNGG 8 cut(s) 283, 928, 2292, 2329, 2522, 2708, 2956, 2999
Bme18I GGWCC 2 cut(s) 790, 2995
BmeRI GACNNNNNGTC 1 cut(s) 585
BmeT110I CYCGRG 1 cut(s) 1062
BmgBI CACGTC 1 cut(s) 440
BmgT120I GGNCC 4 cut(s) 790, 1876, 1897, 2995
BmiI GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2517
BmrFI CCNGG 8 cut(s) 283, 928, 2292, 2329, 2522, 2708, 2956, 2999
BmrI ACTGGG 1 cut(s) 2772
BmsI GCATC 7 cut(s) 1206, 1228, 1439, 1949, 1969, 2158, 2352
BmtI GCTAGC 1 cut(s) 918
BmuI ACTGGG 1 cut(s) 2772
BpmI CTGGAG 2 cut(s) 2562, 2983
Bpu10I CCTNAGC 1 cut(s) 2208
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 3 cut(s) 690, 1144, 2675
BpuMI CCSGG 2 cut(s) 283, 2329
BsaBI GATNNNNATC 2 cut(s) 76, 2133
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 2 cut(s) 112, 1059
BsaJI CCNNGG 7 cut(s) 8, 688, 2028, 2290, 2520, 2532, 2707
BsaWI WCCGGW 2 cut(s) 80, 406
BsaXI ACNNNNNCTCC 2 cut(s) 2871, 2901
Bsc4I CCNNNNNNNGG 6 cut(s) 689, 927, 1076, 1306, 2392, 2961
Bse118I RCCGGY 1 cut(s) 1045
Bse1I ACTGG 7 cut(s) 109, 399, 815, 1924, 2579, 2767, 2966
Bse21I CCTNAGG 1 cut(s) 123
Bse8I GATNNNNATC 2 cut(s) 76, 2133
BseBI CCWGG 6 cut(s) 928, 2292, 2522, 2708, 2956, 2999
BseDI CCNNGG 7 cut(s) 8, 688, 2028, 2290, 2520, 2532, 2707
BseGI GGATG 4 cut(s) 278, 358, 1219, 2149
BseJI GATNNNNATC 2 cut(s) 76, 2133
BseLI CCNNNNNNNGG 6 cut(s) 689, 927, 1076, 1306, 2392, 2961
BseMII CTCAG 6 cut(s) 114, 1317, 2007, 2199, 2217, 2564
BseNI ACTGG 7 cut(s) 109, 399, 815, 1924, 2579, 2767, 2966
BseXI GCAGC 6 cut(s) 49, 756, 973, 1538, 1941, 2213
BsgI GTGCAG 1 cut(s) 1545
Bsh1236I CGCG 1 cut(s) 742
Bsh1285I CGRYCG 1 cut(s) 1183
BshFI GGCC 8 cut(s) 723, 1045, 1878, 1898, 2327, 2355, 2575, 3002
BshNI GGYRCC 2 cut(s) 83, 397
BsiEI CGRYCG 1 cut(s) 1183
BsiHKAI GWGCWC 1 cut(s) 2470
BsiHKCI CYCGRG 1 cut(s) 1062
BsiSI CCGG 6 cut(s) 44, 81, 282, 407, 1046, 2328
BslFI GGGAC 4 cut(s) 45, 592, 2344, 2460
BslI CCNNNNNNNGG 6 cut(s) 689, 927, 1076, 1306, 2392, 2961
BsmAI GTCTC 4 cut(s) 112, 1059, 1665, 2874
BsmFI GGGAC 4 cut(s) 45, 592, 2344, 2460
BsmI GAATGC 3 cut(s) 1662, 2779, 2877
BsnI GGCC 8 cut(s) 723, 1045, 1878, 1898, 2327, 2355, 2575, 3002
Bso31I GGTCTC 2 cut(s) 112, 1059
BsoBI CYCGRG 1 cut(s) 1062
Bsp119I TTCGAA 1 cut(s) 75
Bsp1286I GDGCHC 2 cut(s) 161, 2470
BspACI CCGC 9 cut(s) 161, 234, 237, 246, 265, 268, 364, 788, 2924
BspANI GGCC 8 cut(s) 723, 1045, 1878, 1898, 2327, 2355, 2575, 3002
BspCNI CTCAG 6 cut(s) 115, 1318, 2006, 2200, 2218, 2563
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2517
BspMAI CTGCAG 1 cut(s) 2203
BspMI ACCTGC 1 cut(s) 261
BspOI GCTAGC 1 cut(s) 918
BspPI GGATC 4 cut(s) 621, 634, 898, 1852
BspQI GCTCTTC 1 cut(s) 1725
BspT104I TTCGAA 1 cut(s) 75
BspT107I GGYRCC 2 cut(s) 83, 397
BspTNI GGTCTC 2 cut(s) 112, 1059
BsrFI RCCGGY 1 cut(s) 1045
BsrI ACTGG 7 cut(s) 109, 399, 815, 1924, 2579, 2767, 2966
BssAI RCCGGY 1 cut(s) 1045
BssECI CCNNGG 7 cut(s) 8, 688, 2028, 2290, 2520, 2532, 2707
BssNAI GTATAC 2 cut(s) 755, 1854
BssNI GRCGYC 1 cut(s) 261
BssSI CACGAG 1 cut(s) 1224
BssT1I CCWWGG 3 cut(s) 688, 2028, 2532
Bst1107I GTATAC 2 cut(s) 755, 1854
Bst2BI CACGAG 1 cut(s) 1224
Bst2UI CCWGG 6 cut(s) 928, 2292, 2522, 2708, 2956, 2999
Bst4CI ACNGT 6 cut(s) 658, 697, 1276, 1608, 2488, 2885
Bst6I CTCTTC 3 cut(s) 1725, 1815, 2114
BstACI GRCGYC 1 cut(s) 261
BstAPI GCANNNNNTGC 2 cut(s) 967, 2465
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 6 cut(s) 161, 224, 916, 1469, 2718, 2835
BstDEI CTNAG 8 cut(s) 123, 1326, 1993, 2208, 2226, 2550, 2666, 2932
BstEII GGTNACC 2 cut(s) 285, 2033
BstENI CCTNNNNNAGG 1 cut(s) 925
BstF5I GGATG 4 cut(s) 278, 358, 1219, 2149
BstFNI CGCG 1 cut(s) 742
BstHHI GCGC 3 cut(s) 744, 1356, 1531
BstMAI GTCTC 4 cut(s) 112, 1059, 1665, 2874
BstMCI CGRYCG 1 cut(s) 1183
BstMWI GCNNNNNNNGC 7 cut(s) 243, 267, 961, 967, 1360, 2465, 2657
BstNI CCWGG 6 cut(s) 928, 2292, 2522, 2708, 2956, 2999
BstNSI RCATGY 2 cut(s) 1471, 2405
BstPI GGTNACC 2 cut(s) 285, 2033
BstSCI CCNGG 8 cut(s) 281, 926, 2290, 2327, 2520, 2706, 2954, 2997
BstSFI CTRYAG 1 cut(s) 2199
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 6 cut(s) 49, 756, 973, 1538, 1941, 2213
BstX2I RGATCY 4 cut(s) 626, 890, 1283, 1844
BstXI CCANNNNNNTGG 1 cut(s) 2045
BstYI RGATCY 4 cut(s) 626, 890, 1283, 1844
BstZ17I GTATAC 2 cut(s) 755, 1854
Bsu36I CCTNAGG 1 cut(s) 123
BsuRI GGCC 8 cut(s) 723, 1045, 1878, 1898, 2327, 2355, 2575, 3002
BtgZI GCGATG 1 cut(s) 1497
BtrI CACGTC 1 cut(s) 440
BtsCI GGATG 4 cut(s) 278, 358, 1219, 2149
BtsIMutI CAGTG 7 cut(s) 392, 654, 822, 1613, 2496, 2760, 2890
BveI ACCTGC 1 cut(s) 261
Cac8I GCNNGC 6 cut(s) 161, 224, 916, 1469, 2718, 2835
CaiI CAGNNNCTG 3 cut(s) 2207, 2961, 2998
CfoI GCGC 3 cut(s) 744, 1356, 1531
Cfr10I RCCGGY 1 cut(s) 1045
Cfr13I GGNCC 4 cut(s) 790, 1876, 1897, 2995
CseI GACGC 2 cut(s) 250, 1473
Csp6I GTAC 9 cut(s) 102, 131, 398, 693, 1229, 1290, 2246, 2484, 2507
CviQI GTAC 9 cut(s) 102, 131, 398, 693, 1229, 1290, 2246, 2484, 2507
DdeI CTNAG 8 cut(s) 123, 1326, 1993, 2208, 2226, 2550, 2666, 2932
DraIII CACNNNGTG 1 cut(s) 1213
DriI GACNNNNNGTC 1 cut(s) 585
EaeI YGGCCR 3 cut(s) 721, 2353, 3000
Eam1104I CTCTTC 3 cut(s) 1725, 1815, 2114
Eam1105I GACNNNNNGTC 1 cut(s) 585
EarI CTCTTC 3 cut(s) 1725, 1815, 2114
EciI GGCGGA 1 cut(s) 254
Eco130I CCWWGG 3 cut(s) 688, 2028, 2532
Eco24I GRGCYC 1 cut(s) 161
Eco31I GGTCTC 2 cut(s) 112, 1059
Eco47I GGWCC 2 cut(s) 790, 2995
Eco57I CTGAAG 2 cut(s) 764, 1610
Eco81I CCTNAGG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 1062
Eco91I GGTNACC 2 cut(s) 285, 2033
EcoNI CCTNNNNNAGG 1 cut(s) 925
EcoO109I RGGNCCY 1 cut(s) 2995
EcoO65I GGTNACC 2 cut(s) 285, 2033
EcoRI GAATTC 1 cut(s) 935
EcoRII CCWGG 6 cut(s) 926, 2290, 2520, 2706, 2954, 2997
EcoT14I CCWWGG 3 cut(s) 688, 2028, 2532
EcoT22I ATGCAT 5 cut(s) 1033, 1221, 1473, 1641, 2407
EcoT38I GRGCYC 1 cut(s) 161
ErhI CCWWGG 3 cut(s) 688, 2028, 2532
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 4 cut(s) 45, 592, 2344, 2460
FauI CCCGC 1 cut(s) 168
FauNDI CATATG 2 cut(s) 603, 966
FbaI TGATCA 3 cut(s) 1198, 1264, 2722
FblI GTMKAC 2 cut(s) 754, 1853
Fnu4HI GCNGC 8 cut(s) 38, 235, 268, 745, 962, 1527, 1955, 2202
FokI GGATG 4 cut(s) 265, 365, 1206, 2156
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 8 cut(s) 38, 235, 268, 745, 962, 1527, 1955, 2202
FspBI CTAG 8 cut(s) 915, 1119, 1287, 1374, 1755, 1788, 1860, 2138
GlaI GCGC 3 cut(s) 743, 1355, 1530
GluI GCNGC 8 cut(s) 38, 235, 268, 745, 962, 1527, 1955, 2202
GsuI CTGGAG 2 cut(s) 2562, 2983
HaeIII GGCC 8 cut(s) 723, 1045, 1878, 1898, 2327, 2355, 2575, 3002
HapII CCGG 6 cut(s) 44, 81, 282, 407, 1046, 2328
HgaI GACGC 2 cut(s) 250, 1473
HhaI GCGC 3 cut(s) 744, 1356, 1531
Hin1I GRCGYC 1 cut(s) 261
Hin6I GCGC 3 cut(s) 742, 1354, 1529
HinP1I GCGC 3 cut(s) 742, 1354, 1529
HincII GTYRAC 2 cut(s) 276, 2080
HindII GTYRAC 2 cut(s) 276, 2080
HindIII AAGCTT 3 cut(s) 1676, 2359, 2541
HpaII CCGG 6 cut(s) 44, 81, 282, 407, 1046, 2328
Hpy166II GTNNAC 6 cut(s) 276, 572, 755, 1854, 2080, 2149
Hpy8I GTNNAC 6 cut(s) 276, 572, 755, 1854, 2080, 2149
HpyAV CCTTC 7 cut(s) 830, 981, 1479, 2266, 2362, 2548, 2626
HpyCH4III ACNGT 6 cut(s) 658, 697, 1276, 1608, 2488, 2885
HpyCH4IV ACGT 5 cut(s) 58, 439, 757, 2812, 2840
HpyF10VI GCNNNNNNNGC 7 cut(s) 243, 267, 961, 967, 1360, 2465, 2657
HpyF3I CTNAG 8 cut(s) 123, 1326, 1993, 2208, 2226, 2550, 2666, 2932
HpySE526I ACGT 5 cut(s) 58, 439, 757, 2812, 2840
Hsp92I GRCGYC 1 cut(s) 261
HspAI GCGC 3 cut(s) 742, 1354, 1529
KpnI GGTACC 1 cut(s) 401
Ksp22I TGATCA 3 cut(s) 1198, 1264, 2722
LguI GCTCTTC 1 cut(s) 1725
LmnI GCTCC 7 cut(s) 509, 710, 923, 1302, 2209, 2893, 3013
Lsp1109I GCAGC 6 cut(s) 49, 756, 973, 1538, 1941, 2213
LweI GCATC 7 cut(s) 1206, 1228, 1439, 1949, 1969, 2158, 2352
MaeI CTAG 8 cut(s) 915, 1119, 1287, 1374, 1755, 1788, 1860, 2138
MaeII ACGT 5 cut(s) 58, 439, 757, 2812, 2840
MaeIII GTNAC 8 cut(s) 285, 321, 650, 817, 1959, 2033, 2067, 2395
MfeI CAATTG 1 cut(s) 2240
MflI RGATCY 4 cut(s) 626, 890, 1283, 1844
MhlI GDGCHC 2 cut(s) 161, 2470
MlsI TGGCCA 2 cut(s) 723, 3002
MluNI TGGCCA 2 cut(s) 723, 3002
MlyI GAGTC 1 cut(s) 1961
MmeI TCCRAC 1 cut(s) 2868
Mox20I TGGCCA 2 cut(s) 723, 3002
Mph1103I ATGCAT 5 cut(s) 1033, 1221, 1473, 1641, 2407
MroXI GAANNNNTTC 3 cut(s) 777, 935, 2372
MscI TGGCCA 2 cut(s) 723, 3002
MseI TTAA 9 cut(s) 428, 1008, 1026, 1725, 2054, 2279, 2681, 2949, 3015
MslI CAYNNNNRTG 5 cut(s) 177, 438, 1521, 1740, 2154
Msp20I TGGCCA 2 cut(s) 723, 3002
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 6 cut(s) 44, 81, 282, 407, 1046, 2328
MspR9I CCNGG 8 cut(s) 283, 928, 2292, 2329, 2522, 2708, 2956, 2999
MunI CAATTG 1 cut(s) 2240
Mva1269I GAATGC 3 cut(s) 1662, 2779, 2877
MvaI CCWGG 6 cut(s) 928, 2292, 2522, 2708, 2956, 2999
MvnI CGCG 1 cut(s) 742
MwoI GCNNNNNNNGC 7 cut(s) 243, 267, 961, 967, 1360, 2465, 2657
NciI CCSGG 2 cut(s) 283, 2329
NdeI CATATG 2 cut(s) 603, 966
NheI GCTAGC 1 cut(s) 914
NlaIV GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2517
NmuCI GTSAC 7 cut(s) 285, 321, 650, 817, 1959, 2033, 2067
NsiI ATGCAT 5 cut(s) 1033, 1221, 1473, 1641, 2407
NspI RCATGY 2 cut(s) 1471, 2405
NspV TTCGAA 1 cut(s) 75
OliI CACNNNNGTG 2 cut(s) 438, 2154
PaeI GCATGC 1 cut(s) 1471
PaeR7I CTCGAG 1 cut(s) 1062
PciSI GCTCTTC 1 cut(s) 1725
PctI GAATGC 3 cut(s) 1662, 2779, 2877
PdmI GAANNNNTTC 3 cut(s) 777, 935, 2372
PflFI GACNNNGTC 1 cut(s) 1608
PflMI CCANNNNNTGG 3 cut(s) 689, 2392, 2961
PkrI GCNGC 8 cut(s) 39, 236, 269, 746, 963, 1528, 1956, 2203
Ple19I CGATCG 1 cut(s) 1183
PleI GAGTC 1 cut(s) 1961
PpsI GAGTC 1 cut(s) 1961
PpuMI RGGWCCY 1 cut(s) 2995
PshBI ATTAAT 1 cut(s) 1026
PsiI TTATAA 2 cut(s) 701, 2007
Psp5II RGGWCCY 1 cut(s) 2995
Psp6I CCWGG 6 cut(s) 926, 2290, 2520, 2706, 2954, 2997
PspEI GGTNACC 2 cut(s) 285, 2033
PspGI CCWGG 6 cut(s) 926, 2290, 2520, 2706, 2954, 2997
PspN4I GGNNCC 6 cut(s) 85, 399, 505, 628, 792, 2517
PspPI GGNCC 4 cut(s) 790, 1876, 1897, 2995
PspPPI RGGWCCY 1 cut(s) 2995
PsrI GAACNNNNNNTAC 2 cut(s) 2499, 2531
PstI CTGCAG 1 cut(s) 2203
PstNI CAGNNNCTG 3 cut(s) 2207, 2961, 2998
PsuI RGATCY 4 cut(s) 626, 890, 1283, 1844
PsyI GACNNNGTC 1 cut(s) 1608
PvuI CGATCG 1 cut(s) 1183
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 9 cut(s) 103, 132, 399, 694, 1230, 1291, 2247, 2485, 2508
RsaNI GTAC 9 cut(s) 102, 131, 398, 693, 1229, 1290, 2246, 2484, 2507
RseI CAYNNNNRTG 5 cut(s) 177, 438, 1521, 1740, 2154
SapI GCTCTTC 1 cut(s) 1725
SaqAI TTAA 9 cut(s) 428, 1008, 1026, 1725, 2054, 2279, 2681, 2949, 3015
SatI GCNGC 8 cut(s) 38, 235, 268, 745, 962, 1527, 1955, 2202
Sau96I GGNCC 4 cut(s) 790, 1876, 1897, 2995
ScaI AGTACT 3 cut(s) 103, 2485, 2508
SchI GAGTC 1 cut(s) 1961
ScrFI CCNGG 8 cut(s) 283, 928, 2292, 2329, 2522, 2708, 2956, 2999
SduI GDGCHC 2 cut(s) 161, 2470
SfaNI GCATC 7 cut(s) 1206, 1228, 1439, 1949, 1969, 2158, 2352
SfcI CTRYAG 1 cut(s) 2199
Sfr274I CTCGAG 1 cut(s) 1062
SfuI TTCGAA 1 cut(s) 75
SinI GGWCC 2 cut(s) 790, 2995
SlaI CTCGAG 1 cut(s) 1062
SmiMI CAYNNNNRTG 5 cut(s) 177, 438, 1521, 1740, 2154
SmlI CTYRAG 4 cut(s) 705, 1062, 1159, 2654
SmoI CTYRAG 4 cut(s) 705, 1062, 1159, 2654
SphI GCATGC 1 cut(s) 1471
SsiI CCGC 9 cut(s) 161, 234, 237, 246, 265, 268, 364, 788, 2924
SspI AATATT 1 cut(s) 2599
SspMI CTAG 8 cut(s) 915, 1119, 1287, 1374, 1755, 1788, 1860, 2138
StyD4I CCNGG 8 cut(s) 281, 926, 2290, 2327, 2520, 2706, 2954, 2997
StyI CCWWGG 3 cut(s) 688, 2028, 2532
TaaI ACNGT 6 cut(s) 658, 697, 1276, 1608, 2488, 2885
TaiI ACGT 5 cut(s) 61, 442, 760, 2815, 2843
TaqI TCGA 4 cut(s) 75, 1063, 1433, 2366
TatI WGTACW 3 cut(s) 101, 2483, 2506
TauI GCSGC 2 cut(s) 237, 270
Tru1I TTAA 9 cut(s) 428, 1008, 1026, 1725, 2054, 2279, 2681, 2949, 3015
Tru9I TTAA 9 cut(s) 428, 1008, 1026, 1725, 2054, 2279, 2681, 2949, 3015
TscAI CASTG 7 cut(s) 399, 661, 822, 1613, 2503, 2767, 2890
TseFI GTSAC 7 cut(s) 285, 321, 650, 817, 1959, 2033, 2067
TseI GCWGC 6 cut(s) 37, 744, 961, 1526, 1954, 2201
Tsp45I GTSAC 7 cut(s) 285, 321, 650, 817, 1959, 2033, 2067
TspGWI ACGGA 1 cut(s) 2865
TspRI CASTG 7 cut(s) 399, 661, 822, 1613, 2503, 2767, 2890
Tth111I GACNNNGTC 1 cut(s) 1608
Van91I CCANNNNNTGG 3 cut(s) 689, 2392, 2961
VpaK11BI GGWCC 2 cut(s) 790, 2995
VspI ATTAAT 1 cut(s) 1026
XagI CCTNNNNNAGG 1 cut(s) 925
XapI RAATTY 5 cut(s) 935, 983, 1342, 1491, 1934
XbaI TCTAGA 3 cut(s) 1787, 1859, 2137
XceI RCATGY 2 cut(s) 1471, 2405
XhoI CTCGAG 1 cut(s) 1062
XmiI GTMKAC 2 cut(s) 754, 1853
XmnI GAANNNNTTC 3 cut(s) 777, 935, 2372
XspI CTAG 8 cut(s) 915, 1119, 1287, 1374, 1755, 1788, 1860, 2138
ZrmI AGTACT 3 cut(s) 103, 2485, 2508
Zsp2I ATGCAT 5 cut(s) 1033, 1221, 1473, 1641, 2407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.