MD07G1067200.v1.1

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009713
Physical Location & Seq
Reverse (-)
6314739 .. 6320682
5944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1067200.v1.1.491

Sequence Viewer

Length: 2994 bp
ATGCATTCTGGGAAGCATGAAAATTTCAATAAAAAATCTCCAGCTTCTAGATTACATGCCCCCCACCGATTTGAATTGGGTCCTGACCCGTTTAGTAATAGTGGTAGGAGGAGTAATATGCGACTCGAGGCGGCTGATCAGTCTATTGGGAGGAATTTGAGCCCTCGGGGATTGCCAGGGGATGTGGGTTTGAGTCAAAGGACTGATTCTGTTGAAAGGAGGGACTATGGTTGGCATTTGGGTCGTGGAAGGACTGATCGGGTGAGGCGGAGGTCAAGATCCAGGTCACCACTGCAGCCTTTTGGTGACATGCGGAAAAGGCCACATTTTGATGAGGGAGTCGGGGTTTTGCCTAGGAATTATTCGCCACCTCTACTTGCACCTGTTCCTTTGGAGTTGCAACACAGACCTGGATTGGCTGAGCCTGCAATATATAGTGTGAATGACGATTCAAATAGTAGACGAGTTTACGGGTCTGAGAATAATGACTCTAGAATCAGTAATGAGGAATTGAATGGAAGCAGATTGTCAGTTGGTGGTACACATGAGGCATTGAGTAAAAAATCAATGCATGTGGAAGATGGTGCGGTAAGAGGATCAGAACCCGCACTTATCCAAGATAACACTGTACTAGGAACATATTGGCCACCTCCGGACCTGCATCCTGTGACAACCCCTGCAGAAGGCAGTGAACACCTACCATCATCATCACGGACTATGAATGTACGCCACTTTCAGCAAGAAAGGCCTCAGTACATGGATCCTGTTGCTTTGGATAGATTACCAGTGACAGAATCCTACAAAGGAGAGAAACACATTTTTCCTTCAAGGGATGGTTTGTACCCAATGATGTCAAGTGCTCTTAATACGGATTTTCATGCTAGAACCTCCACAGACATAAGGAGTGACTTTCAAGATTCCTACCGAGGTGGCCATCACTTACCTTCTATGGATGAGTTTTCAAGTAGCAGCCGGAAGTTTATAGAGTCCTCGAGCATTAATGCATATAGGCAAAGGCCACCGGCAGATTATTCTAGAGATCCTGATTCTGGCAAGAGGAGTCTGGCAATTTACCAGAGTTATAGTCCTACTAGAGGTGAGCATGATGATTATTATTTTCCCAAATCAAGGGGAATGCCAGTTGATGATCGCGGATATCCATCTGATGACTTACATAAAATGATGCCTCCACGACCTCAACTTGACTATGATCATACTCGAATGGTTTATGAGCATCAAAATTTGTCAAGACCTAGTATTATGAACCCTGTTATGGATAGAATGGATGATACTGAGGGATTTTCTGGAAATTCAAGAAGGGGCATTATGATGAATAATCCTACTTTGCAAAGGCAATCTTTAATAGACTACCCTGATTCAAGAAGAGTATCAGAGACATCAAAGCACGGTGCAGAATATTTGGGTTCAGGACGCACACATGTCAATTTAGGAAGGAGAAATCCACAAGATTATGAACTATCCCATTTCAGGGCATCCCAAGATTTTCAGGTCGCACATCCAAAAGAAGATTATGGTTTTGAAAGAGATGTGAATATGAAGTATCAGGAAAGGCTGTCTCCTGTATCAAAATATGAGTCAGAAATGCGTGGACATCCTGCTGGAATGCAGATTATGAGAGAGGAGCTTGGAATATATGAACCATCAGATAGGATGCTCAAAAGAAACTATGCTACTGAAGAACATATGAGTACACATAATCCTAGAACTATTATGTCTAGTAAGTGGACATCTAGTGAATTCCAAGATTTATATGATAGTGGTGAAGAGTGGAATGATGGAGATGTAGGCAGTTTCTGTACATCTGCATCAGCTGGATTTGATCATGATAGATACAGTAAGGCTAAGAGGGTATTTGTTGGGCGCCGTCATGATGAATATGAATATGATGATTGGTTGCCATCTCAACACTCTTTTGAAAATGCGCAAAGGCATTCAGTCAGATTCTATAAACAAGGTGATCGATATATCAAGGGTCATCAAAAGTCTGGTCCATTAAGTAGGCATAAGCCGCGCCACACTGATATAAAAAGTGGTGTTCACAAACAGCACCGAGTTTGGAAAAGACATGATAGTTATTTAGAAGATGTCCATGCAAGTGATGGTACTGATGTTGATTTATCAGAAAATGGGCTGAGCTCTGCAGGTCCTGAGCCATCCGAGGACTCTGAGGAGTTTATGCAAATGGTAAATGAAGCCTTTTTAACATTTTCTAAAAAGCTAAATATGAACTCGGCTGTTCGGAGAAGATACAAGGAACAAGGAAAGGCTGGAACTTTGTTCTGCATCGTATGTGGCCGAAGCTTGTCGAAGGAGTTCATGGACACTCAGCGCCTGGTAACACATGCATATATGTCTCACAAAGTTGGGCTGAGGGCACAGCACTTGGGTCTTCTAAAAGCAGTTTGTGTTCTGTTGGGATGGAGTACCATTGTCCCCCCTGACACGGTCACTTGGGTTCCCCAGGTCCTGCCCAAGGCAGAAGCTTTGGCTCAGAAGGAAGATTTGATTCTCTGGCCTCCTGTAATTGTTGTCCACAATATTTCTATGTCCGACAACAATCCTGAATGTTGGAAGGTTGTATCCATGGAGGCGCTCGAGGCTTTTCTAAGAAGTAACGGTTTAATCAAGGGAAGAATCAAGATTTGCCTGGGCAAGCCTGCTGACCAAAGTGTTTTGGTGGTGAAGTTCTTGGGAACGTTTACTGGGCTTGGGGACGCGGAGAGGATTCAGAAGCATTTTGCAGAGCATAAGCGTGGCAGAGTAGATTTTGAGCGAGCAACATCCTCGAATGGCAAAATTGTGGAAGCAGCAATGCAGGGAGACAACCCGGAGGAGCGATTTCTATATGGGTATATGGGCATCGTAGAGGACTTGGATAAAGTGGATTTCCATACCAGGAGCTGGACTGTGATAAAGAGCAAGAAGGAGATTCAGGACCTGGCCAATGCTCCCGTCAAACCTGGTGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

998

Amino Acids

113.63

Weight (kDa)

7.79

Isoelectric Point (pI)

58.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 840 - 966 5.8e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1944
Acc36I ACCTGC 2 cut(s) 666, 2153
AccB1I GGYRCC 1 cut(s) 1881
AccB7I CCANNNNNTGG 1 cut(s) 2922
AccI GTMKAC 1 cut(s) 460
AccII CGCG 3 cut(s) 1152, 2032, 2738
AccIII TCCGGA 1 cut(s) 652
AciI CCGC 8 cut(s) 131, 268, 313, 587, 606, 1152, 2030, 2738
AclI AACGTT 1 cut(s) 2717
AclWI GGATC 5 cut(s) 273, 604, 755, 768, 1034
AcoI YGGCCR 4 cut(s) 644, 931, 2314, 2961
AcsI RAATTY 5 cut(s) 22, 154, 1240, 1309, 1757
AcuI CTGAAG 1 cut(s) 1716
AcyI GRCGYC 1 cut(s) 1882
AfaI GTAC 9 cut(s) 541, 630, 726, 755, 842, 1711, 1819, 2125, 2446
AflIII ACRYGT 1 cut(s) 1438
AjnI CCWGG 9 cut(s) 175, 281, 409, 2352, 2481, 2667, 2915, 2958, 2980
AluBI AGCT 8 cut(s) 44, 1645, 1832, 2157, 2239, 2322, 2504, 2922
AluI AGCT 8 cut(s) 44, 1645, 1832, 2157, 2239, 2322, 2504, 2922
Alw21I GWGCWC 2 cut(s) 862, 2159
Alw26I GTCTC 4 cut(s) 1388, 1581, 2379, 2835
AlwI GGATC 5 cut(s) 273, 604, 755, 768, 1034
AlwNI CAGNNNCTG 6 cut(s) 1815, 2168, 2353, 2488, 2922, 2959
Ama87I CYCGRG 4 cut(s) 125, 165, 991, 2615
Aor13HI TCCGGA 1 cut(s) 652
AoxI GGCC 8 cut(s) 320, 644, 746, 931, 1016, 2314, 2534, 2961
ApeKI GCWGC 3 cut(s) 295, 969, 2828
ApoI RAATTY 5 cut(s) 22, 154, 1240, 1309, 1757
ArsI GACNNNNNNTTYG 4 cut(s) 1580, 1612, 1993, 2025
AseI ATTAAT 1 cut(s) 999
Asp700I GAANNNNTTC 1 cut(s) 2333
AspA2I CCTAGG 1 cut(s) 353
AspLEI GCGC 5 cut(s) 1884, 1945, 2034, 2352, 2614
AspS9I GGNCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
AsuC2I CCSGG 1 cut(s) 2849
AsuHPI GGTGA 7 cut(s) 274, 279, 317, 1109, 1793, 1988, 2713
AvaI CYCGRG 4 cut(s) 125, 165, 991, 2615
AvaII GGWCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
AvrII CCTAGG 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 2398
BaeI ACNNNNGTAYC 2 cut(s) 2115, 2148
BalI TGGCCA 3 cut(s) 646, 933, 2963
BamHI GGATCC 1 cut(s) 760
BanI GGYRCC 1 cut(s) 1881
BanII GRGCYC 2 cut(s) 164, 2159
BbsI GAAGAC 1 cut(s) 2402
Bbv12I GWGCWC 2 cut(s) 862, 2159
BbvCI CCTCAGC 1 cut(s) 2390
BbvI GCAGC 3 cut(s) 307, 981, 2840
BceAI ACGGC 1 cut(s) 1869
BcgI CGANNNNNNTGC 4 cut(s) 224, 258, 2787, 2821
BciT130I CCWGG 9 cut(s) 177, 283, 411, 2354, 2483, 2669, 2917, 2960, 2982
BciVI GTATCC 1 cut(s) 2611
BclI TGATCA 3 cut(s) 136, 1210, 1840
BcnI CCSGG 1 cut(s) 2849
BcoDI GTCTC 4 cut(s) 1388, 1581, 2379, 2835
BfmI CTRYAG 3 cut(s) 293, 678, 2160
BfoI RGCGCY 3 cut(s) 1885, 2353, 2615
BfuAI ACCTGC 2 cut(s) 666, 2153
BfuI GTATCC 1 cut(s) 2611
BisI GCNGC 5 cut(s) 132, 296, 970, 2030, 2829
BlnI CCTAGG 1 cut(s) 353
BlpI GCTNAGC 2 cut(s) 420, 2153
BlsI GCNGC 5 cut(s) 133, 297, 971, 2031, 2830
Bme18I GGWCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
BmeT110I CYCGRG 4 cut(s) 125, 165, 991, 2615
BmgT120I GGNCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
BmiI GGNNCC 4 cut(s) 81, 762, 1883, 2478
BmrI ACTGGG 1 cut(s) 2733
BmsI GCATC 8 cut(s) 670, 1173, 1243, 1502, 1662, 1835, 2313, 2889
BmuI ACTGGG 1 cut(s) 2733
BpiI GAAGAC 1 cut(s) 2402
BpmI CTGGAG 1 cut(s) 24
Bpu10I CCTNAGC 2 cut(s) 2169, 2390
Bpu1102I GCTNAGC 2 cut(s) 420, 2153
BpuMI CCSGG 1 cut(s) 2849
Bsa29I ATCGAT 1 cut(s) 1981
BsaBI GATNNNNATC 1 cut(s) 1159
BsaHI GRCGYC 1 cut(s) 1882
BsaJI CCNNGG 9 cut(s) 164, 176, 353, 925, 2178, 2481, 2493, 2604, 2668
BsaWI WCCGGW 1 cut(s) 652
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bse118I RCCGGY 1 cut(s) 1021
Bse1I ACTGG 3 cut(s) 785, 1139, 2728
Bse3DI GCAATG 1 cut(s) 2838
Bse8I GATNNNNATC 1 cut(s) 1159
BseAI TCCGGA 1 cut(s) 652
BseBI CCWGG 9 cut(s) 177, 283, 411, 2354, 2483, 2669, 2917, 2960, 2982
BseCI ATCGAT 1 cut(s) 1981
BseDI CCNNGG 9 cut(s) 164, 176, 353, 925, 2178, 2481, 2493, 2604, 2668
BseJI GATNNNNATC 1 cut(s) 1159
BseMI GCAATG 1 cut(s) 2838
BseNI ACTGG 3 cut(s) 785, 1139, 2728
BseRI GAGGAG 5 cut(s) 124, 1072, 1655, 2204, 2867
BseSI GKGCMC 1 cut(s) 2398
BseXI GCAGC 3 cut(s) 307, 981, 2840
BsgI GTGCAG 1 cut(s) 1431
Bsh1236I CGCG 3 cut(s) 1152, 2032, 2738
BshFI GGCC 8 cut(s) 322, 646, 748, 933, 1018, 2316, 2536, 2963
BshNI GGYRCC 1 cut(s) 1881
BshVI ATCGAT 1 cut(s) 1981
BsiHKAI GWGCWC 2 cut(s) 862, 2159
BsiHKCI CYCGRG 4 cut(s) 125, 165, 991, 2615
BsiSI CCGG 4 cut(s) 653, 973, 1022, 2849
BslFI GGGAC 3 cut(s) 236, 2438, 2747
BsmAI GTCTC 4 cut(s) 1388, 1581, 2379, 2835
BsmFI GGGAC 3 cut(s) 236, 2438, 2747
BsmI GAATGC 4 cut(s) 4, 1140, 1629, 1951
BsnI GGCC 8 cut(s) 322, 646, 748, 933, 1018, 2316, 2536, 2963
BsoBI CYCGRG 4 cut(s) 125, 165, 991, 2615
Bsp1286I GDGCHC 4 cut(s) 164, 862, 2159, 2398
Bsp13I TCCGGA 1 cut(s) 652
Bsp1407I TGTACA 1 cut(s) 1817
Bsp1720I GCTNAGC 2 cut(s) 420, 2153
Bsp19I CCATGG 1 cut(s) 2604
BspACI CCGC 8 cut(s) 131, 268, 313, 587, 606, 1152, 2030, 2738
BspANI GGCC 8 cut(s) 322, 646, 748, 933, 1018, 2316, 2536, 2963
BspDI ATCGAT 1 cut(s) 1981
BspEI TCCGGA 1 cut(s) 652
BspFNI CGCG 3 cut(s) 1152, 2032, 2738
BspHI TCATGA 2 cut(s) 1843, 1888
BspLI GGNNCC 4 cut(s) 81, 762, 1883, 2478
BspMAI CTGCAG 3 cut(s) 297, 682, 2164
BspMI ACCTGC 2 cut(s) 666, 2153
BspPI GGATC 5 cut(s) 273, 604, 755, 768, 1034
BspT107I GGYRCC 1 cut(s) 1881
BsrDI GCAATG 1 cut(s) 2838
BsrFI RCCGGY 1 cut(s) 1021
BsrGI TGTACA 1 cut(s) 1817
BsrI ACTGG 3 cut(s) 785, 1139, 2728
BssAI RCCGGY 1 cut(s) 1021
BssECI CCNNGG 9 cut(s) 164, 176, 353, 925, 2178, 2481, 2493, 2604, 2668
BssNI GRCGYC 1 cut(s) 1882
BssT1I CCWWGG 3 cut(s) 353, 2493, 2604
Bst2UI CCWGG 9 cut(s) 177, 283, 411, 2354, 2483, 2669, 2917, 2960, 2982
Bst4CI ACNGT 6 cut(s) 628, 1409, 1856, 2467, 2639, 2929
Bst6I CTCTTC 2 cut(s) 1378, 1779
BstACI GRCGYC 1 cut(s) 1882
BstAUI TGTACA 1 cut(s) 1817
BstC8I GCNNGC 4 cut(s) 426, 2675, 2679, 2796
BstDSI CCRYGG 1 cut(s) 2604
BstEII GGTNACC 1 cut(s) 285
BstENI CCTNNNNNAGG 3 cut(s) 681, 1092, 2492
BstFNI CGCG 3 cut(s) 1152, 2032, 2738
BstH2I RGCGCY 3 cut(s) 1885, 2353, 2615
BstHHI GCGC 5 cut(s) 1884, 1945, 2034, 2352, 2614
BstMAI GTCTC 4 cut(s) 1388, 1581, 2379, 2835
BstMWI GCNNNNNNNGC 5 cut(s) 319, 425, 745, 2029, 2618
BstNI CCWGG 9 cut(s) 177, 283, 411, 2354, 2483, 2669, 2917, 2960, 2982
BstNSI RCATGY 5 cut(s) 59, 313, 575, 1442, 2366
BstPI GGTNACC 1 cut(s) 285
BstSFI CTRYAG 3 cut(s) 293, 678, 2160
BstSLI GKGCMC 1 cut(s) 2398
BstUI CGCG 3 cut(s) 1152, 2032, 2738
BstV1I GCAGC 3 cut(s) 307, 981, 2840
BstV2I GAAGAC 1 cut(s) 2402
BstX2I RGATCY 3 cut(s) 278, 760, 1039
BstYI RGATCY 3 cut(s) 278, 760, 1039
Bsu15I ATCGAT 1 cut(s) 1981
BsuI GTATCC 1 cut(s) 2611
BsuRI GGCC 8 cut(s) 322, 646, 748, 933, 1018, 2316, 2536, 2963
BsuTUI ATCGAT 1 cut(s) 1981
BtgI CCRYGG 1 cut(s) 2604
BtsI GCAGTG 2 cut(s) 290, 694
BtsIMutI CAGTG 5 cut(s) 290, 624, 694, 792, 2037
BveI ACCTGC 2 cut(s) 666, 2153
Cac8I GCNNGC 4 cut(s) 426, 2675, 2679, 2796
CaiI CAGNNNCTG 6 cut(s) 1815, 2168, 2353, 2488, 2922, 2959
CciI TCATGA 2 cut(s) 1843, 1888
CfoI GCGC 5 cut(s) 1884, 1945, 2034, 2352, 2614
Cfr10I RCCGGY 1 cut(s) 1021
Cfr13I GGNCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
ClaI ATCGAT 1 cut(s) 1981
CseI GACGC 2 cut(s) 1440, 2744
CsiI ACCWGGT 1 cut(s) 2980
Csp6I GTAC 9 cut(s) 540, 629, 725, 754, 841, 1710, 1818, 2124, 2445
CspCI CAANNNNNGTGG 2 cut(s) 555, 590
CviQI GTAC 9 cut(s) 540, 629, 725, 754, 841, 1710, 1818, 2124, 2445
DinI GGCGCC 1 cut(s) 1883
EaeI YGGCCR 4 cut(s) 644, 931, 2314, 2961
Eam1104I CTCTTC 2 cut(s) 1378, 1779
EarI CTCTTC 2 cut(s) 1378, 1779
EciI GGCGGA 1 cut(s) 283
Ecl136II GAGCTC 1 cut(s) 2157
Eco130I CCWWGG 3 cut(s) 353, 2493, 2604
Eco147I AGGCCT 1 cut(s) 748
Eco24I GRGCYC 2 cut(s) 164, 2159
Eco32I GATATC 1 cut(s) 1157
Eco47I GGWCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
Eco53kI GAGCTC 1 cut(s) 2157
Eco57I CTGAAG 1 cut(s) 1716
Eco88I CYCGRG 4 cut(s) 125, 165, 991, 2615
Eco91I GGTNACC 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 2157
EcoNI CCTNNNNNAGG 3 cut(s) 681, 1092, 2492
EcoO109I RGGNCCY 4 cut(s) 80, 2165, 2485, 2956
EcoO65I GGTNACC 1 cut(s) 285
EcoRI GAATTC 1 cut(s) 1757
EcoRII CCWGG 9 cut(s) 175, 281, 409, 2352, 2481, 2667, 2915, 2958, 2980
EcoRV GATATC 1 cut(s) 1157
EcoT14I CCWWGG 3 cut(s) 353, 2493, 2604
EcoT22I ATGCAT 4 cut(s) 6, 573, 1006, 2368
EcoT38I GRGCYC 2 cut(s) 164, 2159
EgeI GGCGCC 1 cut(s) 1883
EheI GGCGCC 1 cut(s) 1883
ErhI CCWWGG 3 cut(s) 353, 2493, 2604
FalI AAGNNNNNCTT 2 cut(s) 1342, 1374
FaqI GGGAC 3 cut(s) 236, 2438, 2747
FauI CCCGC 1 cut(s) 613
FauNDI CATATG 1 cut(s) 1704
FbaI TGATCA 3 cut(s) 136, 1210, 1840
FblI GTMKAC 1 cut(s) 460
Fnu4HI GCNGC 5 cut(s) 132, 296, 970, 2030, 2829
FriOI GRGCYC 2 cut(s) 164, 2159
Fsp4HI GCNGC 5 cut(s) 132, 296, 970, 2030, 2829
FspI TGCGCA 1 cut(s) 1944
GlaI GCGC 5 cut(s) 1883, 1944, 2033, 2351, 2613
GluI GCNGC 5 cut(s) 132, 296, 970, 2030, 2829
GsuI CTGGAG 1 cut(s) 24
HaeII RGCGCY 3 cut(s) 1885, 2353, 2615
HaeIII GGCC 8 cut(s) 322, 646, 748, 933, 1018, 2316, 2536, 2963
HapII CCGG 4 cut(s) 653, 973, 1022, 2849
HgaI GACGC 2 cut(s) 1440, 2744
HhaI GCGC 5 cut(s) 1884, 1945, 2034, 2352, 2614
Hin1I GRCGYC 1 cut(s) 1882
Hin6I GCGC 5 cut(s) 1882, 1943, 2032, 2350, 2612
HinP1I GCGC 5 cut(s) 1882, 1943, 2032, 2350, 2612
HindIII AAGCTT 2 cut(s) 2320, 2502
HpaII CCGG 4 cut(s) 653, 973, 1022, 2849
HphI GGTGA 7 cut(s) 274, 279, 317, 1109, 1793, 1988, 2713
HpyCH4III ACNGT 6 cut(s) 628, 1409, 1856, 2467, 2639, 2929
HpyCH4IV ACGT 1 cut(s) 2717
HpyF10VI GCNNNNNNNGC 5 cut(s) 319, 425, 745, 2029, 2618
HpySE526I ACGT 1 cut(s) 2717
Hsp92I GRCGYC 1 cut(s) 1882
HspAI GCGC 5 cut(s) 1882, 1943, 2032, 2350, 2612
KasI GGCGCC 1 cut(s) 1881
Kpn2I TCCGGA 1 cut(s) 652
Ksp22I TGATCA 3 cut(s) 136, 1210, 1840
LmnI GCTCC 4 cut(s) 1642, 2854, 2919, 2974
Lsp1109I GCAGC 3 cut(s) 307, 981, 2840
LweI GCATC 8 cut(s) 670, 1173, 1243, 1502, 1662, 1835, 2313, 2889
MabI ACCWGGT 1 cut(s) 2980
MaeII ACGT 1 cut(s) 2717
MaeIII GTNAC 8 cut(s) 285, 305, 667, 787, 905, 2356, 2467, 2633
MflI RGATCY 3 cut(s) 278, 760, 1039
MhlI GDGCHC 4 cut(s) 164, 862, 2159, 2398
MlsI TGGCCA 3 cut(s) 646, 933, 2963
MluNI TGGCCA 3 cut(s) 646, 933, 2963
Mly113I GGCGCC 1 cut(s) 1882
MlyI GAGTC 8 cut(s) 117, 202, 348, 482, 995, 1069, 1604, 2177
MmeI TCCRAC 2 cut(s) 2570, 2595
Mox20I TGGCCA 3 cut(s) 646, 933, 2963
Mph1103I ATGCAT 4 cut(s) 6, 573, 1006, 2368
MroI TCCGGA 1 cut(s) 652
MroXI GAANNNNTTC 1 cut(s) 2333
MscI TGGCCA 3 cut(s) 646, 933, 2963
MseI TTAA 6 cut(s) 864, 999, 1361, 2015, 2222, 2642
MslI CAYNNNNRTG 4 cut(s) 330, 1328, 2115, 2772
Msp20I TGGCCA 3 cut(s) 646, 933, 2963
MspA1I CMGCKG 1 cut(s) 1832
MspI CCGG 4 cut(s) 653, 973, 1022, 2849
Mva1269I GAATGC 4 cut(s) 4, 1140, 1629, 1951
MvaI CCWGG 9 cut(s) 177, 283, 411, 2354, 2483, 2669, 2917, 2960, 2982
MvnI CGCG 3 cut(s) 1152, 2032, 2738
MwoI GCNNNNNNNGC 5 cut(s) 319, 425, 745, 2029, 2618
NarI GGCGCC 1 cut(s) 1882
NciI CCSGG 1 cut(s) 2849
NcoI CCATGG 1 cut(s) 2604
NdeI CATATG 1 cut(s) 1704
NlaIV GGNNCC 4 cut(s) 81, 762, 1883, 2478
NmeAIII GCCGAG 1 cut(s) 2231
NmuCI GTSAC 6 cut(s) 285, 305, 667, 787, 905, 2467
NsbI TGCGCA 1 cut(s) 1944
NsiI ATGCAT 4 cut(s) 6, 573, 1006, 2368
NspI RCATGY 5 cut(s) 59, 313, 575, 1442, 2366
PaeR7I CTCGAG 3 cut(s) 125, 991, 2615
PagI TCATGA 2 cut(s) 1843, 1888
PceI AGGCCT 1 cut(s) 748
PciI ACATGT 1 cut(s) 1438
PctI GAATGC 4 cut(s) 4, 1140, 1629, 1951
PdmI GAANNNNTTC 1 cut(s) 2333
PflFI GACNNNGTC 1 cut(s) 2465
PflMI CCANNNNNTGG 1 cut(s) 2922
PkrI GCNGC 5 cut(s) 133, 297, 971, 2031, 2830
PleI GAGTC 8 cut(s) 117, 201, 347, 482, 994, 1068, 1603, 2177
PluTI GGCGCC 1 cut(s) 1885
PpsI GAGTC 8 cut(s) 117, 201, 347, 482, 994, 1068, 1603, 2177
PpuMI RGGWCCY 4 cut(s) 80, 2165, 2485, 2956
PscI ACATGT 1 cut(s) 1438
PshBI ATTAAT 1 cut(s) 999
Psp124BI GAGCTC 1 cut(s) 2159
Psp1406I AACGTT 1 cut(s) 2717
Psp5II RGGWCCY 4 cut(s) 80, 2165, 2485, 2956
Psp6I CCWGG 9 cut(s) 175, 281, 409, 2352, 2481, 2667, 2915, 2958, 2980
PspEI GGTNACC 1 cut(s) 285
PspGI CCWGG 9 cut(s) 175, 281, 409, 2352, 2481, 2667, 2915, 2958, 2980
PspN4I GGNNCC 4 cut(s) 81, 762, 1883, 2478
PspPI GGNCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
PspPPI RGGWCCY 4 cut(s) 80, 2165, 2485, 2956
PspXI VCTCGAGB 3 cut(s) 125, 991, 2615
PstI CTGCAG 3 cut(s) 297, 682, 2164
PstNI CAGNNNCTG 6 cut(s) 1815, 2168, 2353, 2488, 2922, 2959
PsuI RGATCY 3 cut(s) 278, 760, 1039
PsyI GACNNNGTC 1 cut(s) 2465
PvuII CAGCTG 1 cut(s) 1832
RsaI GTAC 9 cut(s) 541, 630, 726, 755, 842, 1711, 1819, 2125, 2446
RsaNI GTAC 9 cut(s) 540, 629, 725, 754, 841, 1710, 1818, 2124, 2445
RseI CAYNNNNRTG 4 cut(s) 330, 1328, 2115, 2772
SacI GAGCTC 1 cut(s) 2159
SaqAI TTAA 6 cut(s) 864, 999, 1361, 2015, 2222, 2642
SatI GCNGC 5 cut(s) 132, 296, 970, 2030, 2829
Sau96I GGNCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
SchI GAGTC 8 cut(s) 117, 202, 348, 482, 995, 1069, 1604, 2177
SduI GDGCHC 4 cut(s) 164, 862, 2159, 2398
SexAI ACCWGGT 1 cut(s) 2980
SfaNI GCATC 8 cut(s) 670, 1173, 1243, 1502, 1662, 1835, 2313, 2889
SfcI CTRYAG 3 cut(s) 293, 678, 2160
SfoI GGCGCC 1 cut(s) 1883
Sfr274I CTCGAG 3 cut(s) 125, 991, 2615
SinI GGWCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
SlaI CTCGAG 3 cut(s) 125, 991, 2615
SmiMI CAYNNNNRTG 4 cut(s) 330, 1328, 2115, 2772
SmlI CTYRAG 3 cut(s) 125, 991, 2615
SmoI CTYRAG 3 cut(s) 125, 991, 2615
SseBI AGGCCT 1 cut(s) 748
SsiI CCGC 8 cut(s) 131, 268, 313, 587, 606, 1152, 2030, 2738
SspDI GGCGCC 1 cut(s) 1881
SspI AATATT 2 cut(s) 1418, 2560
SstI GAGCTC 1 cut(s) 2159
StuI AGGCCT 1 cut(s) 748
StyI CCWWGG 3 cut(s) 353, 2493, 2604
TaaI ACNGT 6 cut(s) 628, 1409, 1856, 2467, 2639, 2929
TaiI ACGT 1 cut(s) 2720
TaqI TCGA 7 cut(s) 126, 992, 1219, 1981, 2327, 2616, 2807
TatI WGTACW 4 cut(s) 628, 753, 1709, 1817
TauI GCSGC 2 cut(s) 134, 2032
Tru1I TTAA 6 cut(s) 864, 999, 1361, 2015, 2222, 2642
Tru9I TTAA 6 cut(s) 864, 999, 1361, 2015, 2222, 2642
TscAI CASTG 5 cut(s) 297, 631, 694, 792, 2044
TseFI GTSAC 6 cut(s) 285, 305, 667, 787, 905, 2467
TseI GCWGC 3 cut(s) 295, 969, 2828
Tsp45I GTSAC 6 cut(s) 285, 305, 667, 787, 905, 2467
TspGWI ACGGA 2 cut(s) 727, 884
TspRI CASTG 5 cut(s) 297, 631, 694, 792, 2044
Tth111I GACNNNGTC 1 cut(s) 2465
Van91I CCANNNNNTGG 1 cut(s) 2922
VpaK11BI GGWCC 6 cut(s) 80, 655, 2009, 2165, 2485, 2956
VspI ATTAAT 1 cut(s) 999
XagI CCTNNNNNAGG 3 cut(s) 681, 1092, 2492
XapI RAATTY 5 cut(s) 22, 154, 1240, 1309, 1757
XbaI TCTAGA 3 cut(s) 47, 491, 1034
XceI RCATGY 5 cut(s) 59, 313, 575, 1442, 2366
XcmI CCANNNNNNNNNTGG 1 cut(s) 2117
XhoI CTCGAG 3 cut(s) 125, 991, 2615
XmaJI CCTAGG 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 460
XmnI GAANNNNTTC 1 cut(s) 2333
Zsp2I ATGCAT 4 cut(s) 6, 573, 1006, 2368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.