pycom02g21450

XS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009713
Physical Location & Seq
Forward (+)
19617416 .. 19622460
5045 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21450.1

Sequence Viewer

Length: 2961 bp
ATGCATTCTCGGAAGCATGAGAATTTCAATAAGCAATCCCCAGCTTCTAGAGTACATGCGGTTGCTCCCGACCCGTATAGTAATAGTAGTAGGAGGAGTAATATGCAACTCGAGGCTGCTGATCAGTCTAGTGGGAGGAGTTTAAGCCCTCGCGGATTGCCAGGGGATGTGGGTTTGAGTCAAAGGACTGATTCTATTGAAAGGAGTGACCATGGTTGGCATTTGGGTGGTAGAAGGACTGATCGGGTGAGGCGGAGATCAAGGTCAACTTCACCACCTCTGCCTTTTGGCGGCATGCAGAAAAGGCCACATTTTGATGAAGGAGTTGGGGTTATGCATAGGAATTATTCGTCACCTCCACTTGCACCTGTTTCTTCGGAGTTACAACACAGACCTGAACTTGTAGAGCCTGCAAAATATAGAGTGAATGATGATTCAAATAGTAGACGAGTTTACGGGTCGGAGAACAATGATTCTAGAATCAGCAATGAGGAATTGAATGAAAGCAGATTGTCGGTTGGCGTTACACATGGGACATTGAGTCAGAAATCAATGCATGTGGAAGATGGTGCGGTAAGAGGATTAAAATCCACACTTATGCAAGATAATACAGCACTAGGAACATATTGGCCACCTCCAGACCTGCATCCTGTGATAGCTCCTGCAAAAGATTGTGAACACCTACCATCATCATCACGGAGTACAAATTTACCCCATTTTGAGCATGGACGGCCTCAGTATACGGATCCTGTTGCTTTGGATAGATTACCAGTGACAGAATCCTATAAAGGAGAGAAACCCATTCTTACTTCAAGGGATGGTTTGTACCCCATGGTGTCAGGTGCTCATTATACGGATTTTCGTCCTAGCTCCTCCACAGATGTAAGGAGTGAATTTCAGGATTCTTATAGGGGTGGTCCACACTTACCTTCTATGGATGAATTTTCAAGTAGCAGACGGTTCATTAATGCATATAGACAAAGGCCACCAGCAGATTATCCTAGAGATCCTGATTCTGGCAAGGGGAACCTGGCATTTTACCAGAGTTATAGTCCTAACAGAGGTGAGCATGCAGATTTTTTTTACCGCAAATCAAGGGCAATGCCAGTTGATGATCGCAGATATCCATCTGATGACTTACATAAAATGATGCCTCCACGACCACAACTTGACTATGATAGTACTCAAATGGTTTATAACCATCGAAATTTGTCAAGACCTAGTATTATGAACCCTGTTATGGATAGAATGGGTGATACTGAGGAATTTTCTGGAAATTCAAGAAAGGGCATTATGCTGAATAATCCTACTTTGCAGAGGCAACCTTTATTAGACTACCCTGATTCAAGAAGAATATCAGAGACATCAAAGCATGGTGCGGAATATTCAGGTTCAGAATGCACACATGTCAGTTTAGAAAGGAGAATGTCACAAGATTATGAACTATCCCATTTCAGGGCATCACAAGATTTTCAGGTCGCACATCAGAAAGAAGATTATGGTTTTGAAAGAGATGTTAATATGAAGTATCAGGACAGGCTGTCTTCTGTGTCAAAATATGACTCAGAGATGAGTGGGCATCCTGCTGGAATGCAGATTATGAGAGAGGAGCTTGGAATATATGAACCAGCAGATAGGATGCTTAAAAGAAACTACGCTACTGAAGAAGGTATAAGTACACATAATCCTAGAACTATTGTGTCTAGTAAGTGGACATCTAGAGAATTCCAAGATTCATATGAAAGTGGTGAAGAGTGGAATGATGGAGATTTAGGCAGTTATTATACATCTGCATCAGCTGGATTTGACCATGATAGATACAGTAAGGCTGAGAGGGTATATGTTGGGCACCGTCGTGGTGAATATGAATATGATGATTGGTTGCCATCTCAACACTCTTTTGAACATGAACAAATGCATTCAGTCAGATTCTATAAACACGGTGATCGATATATAAAGGGTCATCAAAAATCTGGTCCATTAAGTAGGCATAAGCTGCATACAGCTGATATAAAAAGCAGTGTTCACAAACAGCACCGAGTTTGGAAAAGACATGATAATTATTTAGAAGATGTCCATGTAAGTGATGGTACTGATGTTGATCAATCAGAAAATGGGCTGAACTCTGCAGGTCCTGAGCCATCCGAGGACTCTGAGGAGTTTATGCAAATGGTAAATGAAGCCTTTTTAACATTTTCTAAAAAGCTAAATATGAACTCGGCTGTTCGGAGAAGATACAAGGAACAAGGGAAGGCTGGTACTTTGTTCTGCATTGTATGTGGCCGAAGTTTGTCGAAGGAATTCATGGACACTCAACGCTTGGTAAGACACGCATATATGTCTCACAGAGTTGGGCTGAGGGCTCAGCACTTGGGTCTTCTAAAAGCAGTTTGTGTTCTGTTGGGGTGGAGTACTGTTGTCCCCCCTGACACGGTCACATGGGCTCCCCAGGTCTTGCCCAGGGCTGAAGCTTTGGCTCAGAAGGAAGATTTGATTCTCTGGCCTCCTGTCATTGTTGTTCACAATATTTCTATGTCAGACAACAATCCTCAAAGTTGGAAGGTTGTATCCATGGAGGCACTCGAGGCTTTTCTAAGAAGTAATGGTTTAATCAAGGGAAGAATCAAGATTTGCCTAGGGAAGCCTGCTGACCAAAGTGTTTTGGTGGTGAAGTTCTTGGGAACTTTTACTGGGCTTGGGGACGCAGAGAGGATTCAGAAGCATTTTGCAGAGCATCGGCGTGGCAGAGTGGATTTTGAGCGAGCAACATCCTCGAATGGAAAAATTGTCGAAGCAGCAATGCAGGGAGACAACGCGGAGGAGCGATTTCTATATGGGTACATGGGCATTGTAGAGGACTTGGATAAGGTGGATTTCCATACCAGGAGCTGGACTGTGATAAAGAGCAAGAAGGAGATTCAGGACCTGGCCAATGCTCCCGTCAAACCTGATGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
XS

Protein Analysis

987

Amino Acids

112.41

Weight (kDa)

7.18

Isoelectric Point (pI)

59.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XS PF03468 829 - 955 2.3e-18 XS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1197
Acc36I ACCTGC 2 cut(s) 651, 2120
AccB1I GGYRCC 1 cut(s) 1848
AccB7I CCANNNNNTGG 1 cut(s) 2889
AccI GTMKAC 2 cut(s) 445, 740
AccII CGCG 2 cut(s) 153, 2816
AciI CCGC 8 cut(s) 59, 153, 253, 291, 572, 1087, 1379, 2816
AclWI GGATC 3 cut(s) 740, 753, 1001
AcoI YGGCCR 3 cut(s) 629, 2281, 2928
AcsI RAATTY 9 cut(s) 22, 706, 893, 941, 1207, 1265, 1276, 1724, 2300
AcuI CTGAAG 2 cut(s) 1683, 2487
AfaI GTAC 9 cut(s) 54, 703, 827, 1183, 1678, 2092, 2260, 2413, 2840
AfiI CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1240, 1455, 1456, 2431, 2889
AflIII ACRYGT 1 cut(s) 1405
AhdI GACNNNNNGTC 2 cut(s) 540, 1540
AjnI CCWGG 6 cut(s) 160, 1029, 2448, 2459, 2882, 2925
AleI CACNNNNGTG 1 cut(s) 1854
Alw21I GWGCWC 1 cut(s) 847
Alw26I GTCTC 3 cut(s) 1355, 2346, 2802
AlwI GGATC 3 cut(s) 740, 753, 1001
AlwNI CAGNNNCTG 3 cut(s) 2135, 2889, 2926
Ama87I CYCGRG 2 cut(s) 110, 2582
AoxI GGCC 7 cut(s) 305, 629, 731, 983, 2281, 2501, 2928
ApeKI GCWGC 3 cut(s) 116, 1996, 2795
ApoI RAATTY 9 cut(s) 22, 706, 893, 941, 1207, 1265, 1276, 1724, 2300
ArsI GACNNNNNNTTYG 2 cut(s) 1960, 1992
AseI ATTAAT 1 cut(s) 966
Asp700I GAANNNNTTC 1 cut(s) 2300
AspA2I CCTAGG 1 cut(s) 2635
AspS9I GGNCC 4 cut(s) 917, 1976, 2132, 2923
AsuHPI GGTGA 9 cut(s) 259, 264, 345, 1076, 1265, 1760, 1871, 1955, 2680
AvaI CYCGRG 2 cut(s) 110, 2582
AvaII GGWCC 4 cut(s) 917, 1976, 2132, 2923
AvrII CCTAGG 1 cut(s) 2635
BaeGI GKGCMC 1 cut(s) 1851
BaeI ACNNNNGTAYC 2 cut(s) 2082, 2115
BalI TGGCCA 2 cut(s) 631, 2930
BamHI GGATCC 1 cut(s) 745
BanI GGYRCC 1 cut(s) 1848
BanII GRGCYC 2 cut(s) 2365, 2446
BbsI GAAGAC 2 cut(s) 1536, 2369
Bbv12I GWGCWC 1 cut(s) 847
BbvCI CCTCAGC 1 cut(s) 2357
BbvI GCAGC 3 cut(s) 103, 1983, 2807
BccI CCATC 9 cut(s) 560, 694, 812, 1135, 1209, 1757, 1894, 2081, 2149
BceAI ACGGC 1 cut(s) 746
BcgI CGANNNNNNTGC 2 cut(s) 2754, 2788
BciT130I CCWGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
BciVI GTATCC 1 cut(s) 2578
BclI TGATCA 2 cut(s) 121, 2101
BcoDI GTCTC 3 cut(s) 1355, 2346, 2802
BfmI CTRYAG 1 cut(s) 2127
BfuAI ACCTGC 2 cut(s) 651, 2120
BfuI GTATCC 1 cut(s) 2578
BisI GCNGC 4 cut(s) 117, 292, 1997, 2796
BlnI CCTAGG 1 cut(s) 2635
BlpI GCTNAGC 1 cut(s) 2364
BlsI GCNGC 4 cut(s) 118, 293, 1998, 2797
BmcAI AGTACT 2 cut(s) 1183, 2413
Bme1390I CCNGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
Bme18I GGWCC 4 cut(s) 917, 1976, 2132, 2923
BmeRI GACNNNNNGTC 2 cut(s) 540, 1540
BmeT110I CYCGRG 2 cut(s) 110, 2582
BmgT120I GGNCC 4 cut(s) 917, 1976, 2132, 2923
BmiI GGNNCC 4 cut(s) 747, 1028, 1850, 2445
BmrFI CCNGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
BmrI ACTGGG 1 cut(s) 2700
BmsI GCATC 7 cut(s) 655, 1140, 1469, 1588, 1629, 1802, 2743
BmuI ACTGGG 1 cut(s) 2700
BpiI GAAGAC 2 cut(s) 1536, 2369
BpmI CTGGAG 1 cut(s) 621
Bpu10I CCTNAGC 2 cut(s) 2136, 2357
Bpu1102I GCTNAGC 1 cut(s) 2364
Bsa29I ATCGAT 1 cut(s) 1948
BsaBI GATNNNNATC 3 cut(s) 586, 1126, 2100
BsaJI CCNNGG 9 cut(s) 161, 211, 831, 2145, 2448, 2459, 2460, 2571, 2635
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 2428, 2458
Bsc4I CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1240, 1455, 1456, 2431, 2889
Bse1I ACTGG 3 cut(s) 770, 1106, 2695
Bse3DI GCAATG 3 cut(s) 493, 1107, 2805
Bse8I GATNNNNATC 3 cut(s) 586, 1126, 2100
BseBI CCWGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
BseCI ATCGAT 1 cut(s) 1948
BseDI CCNNGG 9 cut(s) 161, 211, 831, 2145, 2448, 2459, 2460, 2571, 2635
BseGI GGATG 8 cut(s) 172, 646, 823, 943, 1579, 1644, 2141, 2768
BseJI GATNNNNATC 3 cut(s) 586, 1126, 2100
BseLI CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1240, 1455, 1456, 2431, 2889
BseMI GCAATG 3 cut(s) 493, 1107, 2805
BseMII CTCAG 9 cut(s) 749, 1251, 1578, 1821, 2127, 2145, 2348, 2378, 2492
BseNI ACTGG 3 cut(s) 770, 1106, 2695
BseRI GAGGAG 6 cut(s) 109, 151, 862, 1622, 2171, 2834
BseSI GKGCMC 1 cut(s) 1851
BseXI GCAGC 3 cut(s) 103, 1983, 2807
BseYI CCCAGC 1 cut(s) 40
Bsh1236I CGCG 2 cut(s) 153, 2816
BshFI GGCC 7 cut(s) 307, 631, 733, 985, 2283, 2503, 2930
BshNI GGYRCC 1 cut(s) 1848
BshVI ATCGAT 1 cut(s) 1948
BsiHKAI GWGCWC 1 cut(s) 847
BsiHKCI CYCGRG 2 cut(s) 110, 2582
BslFI GGGAC 3 cut(s) 547, 2405, 2714
BslI CCNNNNNNNGG 8 cut(s) 290, 1016, 1061, 1240, 1455, 1456, 2431, 2889
BsmAI GTCTC 3 cut(s) 1355, 2346, 2802
BsmFI GGGAC 3 cut(s) 547, 2405, 2714
BsmI GAATGC 4 cut(s) 4, 1403, 1596, 1918
BsnI GGCC 7 cut(s) 307, 631, 733, 985, 2283, 2503, 2930
BsoBI CYCGRG 2 cut(s) 110, 2582
Bsp1286I GDGCHC 4 cut(s) 847, 1851, 2365, 2446
Bsp143I GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
Bsp1720I GCTNAGC 1 cut(s) 2364
Bsp19I CCATGG 3 cut(s) 211, 831, 2571
BspACI CCGC 8 cut(s) 59, 153, 253, 291, 572, 1087, 1379, 2816
BspANI GGCC 7 cut(s) 307, 631, 733, 985, 2283, 2503, 2930
BspCNI CTCAG 9 cut(s) 748, 1252, 1577, 1822, 2128, 2146, 2349, 2377, 2491
BspDI ATCGAT 1 cut(s) 1948
BspFNI CGCG 2 cut(s) 153, 2816
BspLI GGNNCC 4 cut(s) 747, 1028, 1850, 2445
BspMAI CTGCAG 1 cut(s) 2131
BspMI ACCTGC 2 cut(s) 651, 2120
BspPI GGATC 3 cut(s) 740, 753, 1001
BspT107I GGYRCC 1 cut(s) 1848
BsrDI GCAATG 3 cut(s) 493, 1107, 2805
BsrI ACTGG 3 cut(s) 770, 1106, 2695
BssECI CCNNGG 9 cut(s) 161, 211, 831, 2145, 2448, 2459, 2460, 2571, 2635
BssMI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
BssNAI GTATAC 1 cut(s) 741
BssT1I CCWWGG 4 cut(s) 211, 831, 2571, 2635
Bst1107I GTATAC 1 cut(s) 741
Bst2UI CCWGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
Bst4CI ACNGT 7 cut(s) 960, 1823, 1853, 1943, 2416, 2434, 2896
Bst6I CTCTTC 1 cut(s) 1746
BstAPI GCANNNNNTGC 1 cut(s) 1996
BstC8I GCNNGC 5 cut(s) 296, 411, 1071, 2646, 2763
BstDSI CCRYGG 3 cut(s) 211, 831, 2571
BstENI CCTNNNNNAGG 1 cut(s) 1059
BstF5I GGATG 8 cut(s) 172, 646, 823, 943, 1579, 1644, 2141, 2768
BstFNI CGCG 2 cut(s) 153, 2816
BstKTI GATC 8 cut(s) 124, 244, 260, 748, 1009, 1117, 1948, 2104
BstMAI GTCTC 3 cut(s) 1355, 2346, 2802
BstMBI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
BstMWI GCNNNNNNNGC 4 cut(s) 304, 730, 1996, 2585
BstNI CCWGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
BstNSI RCATGY 5 cut(s) 59, 298, 560, 1073, 1409
BstSCI CCNGG 6 cut(s) 160, 1029, 2448, 2459, 2882, 2925
BstSFI CTRYAG 1 cut(s) 2127
BstSLI GKGCMC 1 cut(s) 1851
BstUI CGCG 2 cut(s) 153, 2816
BstV1I GCAGC 3 cut(s) 103, 1983, 2807
BstV2I GAAGAC 2 cut(s) 1536, 2369
BstX2I RGATCY 2 cut(s) 745, 1006
BstYI RGATCY 2 cut(s) 745, 1006
BstZ17I GTATAC 1 cut(s) 741
Bsu15I ATCGAT 1 cut(s) 1948
BsuI GTATCC 1 cut(s) 2578
BsuRI GGCC 7 cut(s) 307, 631, 733, 985, 2283, 2503, 2930
BsuTUI ATCGAT 1 cut(s) 1948
BtgI CCRYGG 3 cut(s) 211, 831, 2571
BtsCI GGATG 8 cut(s) 172, 646, 823, 943, 1579, 1644, 2141, 2768
BtsI GCAGTG 1 cut(s) 2026
BtsIMutI CAGTG 2 cut(s) 777, 2026
BveI ACCTGC 2 cut(s) 651, 2120
Cac8I GCNNGC 5 cut(s) 296, 411, 1071, 2646, 2763
CaiI CAGNNNCTG 3 cut(s) 2135, 2889, 2926
Cfr13I GGNCC 4 cut(s) 917, 1976, 2132, 2923
ClaI ATCGAT 1 cut(s) 1948
CseI GACGC 1 cut(s) 2711
Csp6I GTAC 9 cut(s) 53, 702, 826, 1182, 1677, 2091, 2259, 2412, 2839
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviQI GTAC 9 cut(s) 53, 702, 826, 1182, 1677, 2091, 2259, 2412, 2839
DpnI GATC 8 cut(s) 123, 243, 259, 747, 1008, 1116, 1947, 2103
DpnII GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
DriI GACNNNNNGTC 2 cut(s) 540, 1540
EaeI YGGCCR 3 cut(s) 629, 2281, 2928
Eam1104I CTCTTC 1 cut(s) 1746
Eam1105I GACNNNNNGTC 2 cut(s) 540, 1540
EarI CTCTTC 1 cut(s) 1746
EciI GGCGGA 1 cut(s) 268
Eco130I CCWWGG 4 cut(s) 211, 831, 2571, 2635
Eco24I GRGCYC 2 cut(s) 2365, 2446
Eco32I GATATC 1 cut(s) 1124
Eco47I GGWCC 4 cut(s) 917, 1976, 2132, 2923
Eco57I CTGAAG 2 cut(s) 1683, 2487
Eco88I CYCGRG 2 cut(s) 110, 2582
EcoNI CCTNNNNNAGG 1 cut(s) 1059
EcoO109I RGGNCCY 2 cut(s) 2132, 2923
EcoRI GAATTC 2 cut(s) 1724, 2300
EcoRII CCWGG 6 cut(s) 160, 1029, 2448, 2459, 2882, 2925
EcoRV GATATC 1 cut(s) 1124
EcoT14I CCWWGG 4 cut(s) 211, 831, 2571, 2635
EcoT22I ATGCAT 5 cut(s) 6, 339, 558, 973, 1920
EcoT38I GRGCYC 2 cut(s) 2365, 2446
ErhI CCWWGG 4 cut(s) 211, 831, 2571, 2635
FalI AAGNNNNNCTT 2 cut(s) 253, 285
FaqI GGGAC 3 cut(s) 547, 2405, 2714
FauNDI CATATG 1 cut(s) 1738
FbaI TGATCA 2 cut(s) 121, 2101
FblI GTMKAC 2 cut(s) 445, 740
Fnu4HI GCNGC 4 cut(s) 117, 292, 1997, 2796
FokI GGATG 8 cut(s) 179, 633, 830, 950, 1566, 1651, 2128, 2755
FriOI GRGCYC 2 cut(s) 2365, 2446
Fsp4HI GCNGC 4 cut(s) 117, 292, 1997, 2796
GluI GCNGC 4 cut(s) 117, 292, 1997, 2796
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 7 cut(s) 307, 631, 733, 985, 2283, 2503, 2930
HgaI GACGC 1 cut(s) 2711
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HindIII AAGCTT 1 cut(s) 2469
HphI GGTGA 9 cut(s) 259, 264, 345, 1076, 1265, 1760, 1871, 1955, 2680
Hpy99I CGWCG 1 cut(s) 1857
HpyAV CCTTC 9 cut(s) 228, 314, 939, 1661, 2245, 2290, 2476, 2554, 2905
HpyCH4III ACNGT 7 cut(s) 960, 1823, 1853, 1943, 2416, 2434, 2896
HpyF10VI GCNNNNNNNGC 4 cut(s) 304, 730, 1996, 2585
Ksp22I TGATCA 2 cut(s) 121, 2101
Kzo9I GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
LmnI GCTCC 8 cut(s) 70, 664, 875, 1609, 2449, 2821, 2886, 2941
Lsp1109I GCAGC 3 cut(s) 103, 1983, 2807
LweI GCATC 7 cut(s) 655, 1140, 1469, 1588, 1629, 1802, 2743
MaeIII GTNAC 7 cut(s) 206, 351, 381, 523, 772, 1428, 2434
MalI GATC 8 cut(s) 123, 243, 259, 747, 1008, 1116, 1947, 2103
MboI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
MflI RGATCY 2 cut(s) 745, 1006
MhlI GDGCHC 4 cut(s) 847, 1851, 2365, 2446
MlsI TGGCCA 2 cut(s) 631, 2930
MluNI TGGCCA 2 cut(s) 631, 2930
MlyI GAGTC 4 cut(s) 187, 550, 1556, 2144
MmeI TCCRAC 2 cut(s) 441, 2537
Mox20I TGGCCA 2 cut(s) 631, 2930
Mph1103I ATGCAT 5 cut(s) 6, 339, 558, 973, 1920
MroXI GAANNNNTTC 1 cut(s) 2300
MscI TGGCCA 2 cut(s) 631, 2930
MseI TTAA 8 cut(s) 143, 584, 966, 1518, 1644, 1982, 2189, 2609
MslI CAYNNNNRTG 6 cut(s) 225, 315, 596, 1854, 2082, 2739
Msp20I TGGCCA 2 cut(s) 631, 2930
MspA1I CMGCKG 2 cut(s) 1799, 2006
MspR9I CCNGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
Mva1269I GAATGC 4 cut(s) 4, 1403, 1596, 1918
MvaI CCWGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
MvnI CGCG 2 cut(s) 153, 2816
MwoI GCNNNNNNNGC 4 cut(s) 304, 730, 1996, 2585
NcoI CCATGG 3 cut(s) 211, 831, 2571
NdeI CATATG 1 cut(s) 1738
NdeII GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
NlaIV GGNNCC 4 cut(s) 747, 1028, 1850, 2445
NmeAIII GCCGAG 1 cut(s) 2198
NmuCI GTSAC 5 cut(s) 206, 351, 772, 1428, 2434
NsiI ATGCAT 5 cut(s) 6, 339, 558, 973, 1920
NspI RCATGY 5 cut(s) 59, 298, 560, 1073, 1409
OliI CACNNNNGTG 1 cut(s) 1854
PaeI GCATGC 2 cut(s) 298, 1073
PaeR7I CTCGAG 2 cut(s) 110, 2582
PasI CCCWGGG 1 cut(s) 2460
PciI ACATGT 1 cut(s) 1405
PctI GAATGC 4 cut(s) 4, 1403, 1596, 1918
PdmI GAANNNNTTC 1 cut(s) 2300
PflFI GACNNNGTC 1 cut(s) 2432
PflMI CCANNNNNTGG 1 cut(s) 2889
PkrI GCNGC 4 cut(s) 118, 293, 1998, 2797
PleI GAGTC 4 cut(s) 186, 549, 1556, 2144
PpsI GAGTC 4 cut(s) 186, 549, 1556, 2144
PpuMI RGGWCCY 2 cut(s) 2132, 2923
PscI ACATGT 1 cut(s) 1405
PshBI ATTAAT 1 cut(s) 966
PsiI TTATAA 1 cut(s) 1197
Psp5II RGGWCCY 2 cut(s) 2132, 2923
Psp6I CCWGG 6 cut(s) 160, 1029, 2448, 2459, 2882, 2925
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 6 cut(s) 160, 1029, 2448, 2459, 2882, 2925
PspN4I GGNNCC 4 cut(s) 747, 1028, 1850, 2445
PspPI GGNCC 4 cut(s) 917, 1976, 2132, 2923
PspPPI RGGWCCY 2 cut(s) 2132, 2923
PspXI VCTCGAGB 2 cut(s) 110, 2582
PstI CTGCAG 1 cut(s) 2131
PstNI CAGNNNCTG 3 cut(s) 2135, 2889, 2926
PsuI RGATCY 2 cut(s) 745, 1006
PsyI GACNNNGTC 1 cut(s) 2432
PvuII CAGCTG 2 cut(s) 1799, 2006
RsaI GTAC 9 cut(s) 54, 703, 827, 1183, 1678, 2092, 2260, 2413, 2840
RsaNI GTAC 9 cut(s) 53, 702, 826, 1182, 1677, 2091, 2259, 2412, 2839
RseI CAYNNNNRTG 6 cut(s) 225, 315, 596, 1854, 2082, 2739
SaqAI TTAA 8 cut(s) 143, 584, 966, 1518, 1644, 1982, 2189, 2609
SatI GCNGC 4 cut(s) 117, 292, 1997, 2796
Sau3AI GATC 8 cut(s) 121, 241, 257, 745, 1006, 1114, 1945, 2101
Sau96I GGNCC 4 cut(s) 917, 1976, 2132, 2923
ScaI AGTACT 2 cut(s) 1183, 2413
SchI GAGTC 4 cut(s) 187, 550, 1556, 2144
ScrFI CCNGG 6 cut(s) 162, 1031, 2450, 2461, 2884, 2927
SduI GDGCHC 4 cut(s) 847, 1851, 2365, 2446
SfaNI GCATC 7 cut(s) 655, 1140, 1469, 1588, 1629, 1802, 2743
SfcI CTRYAG 1 cut(s) 2127
Sfr274I CTCGAG 2 cut(s) 110, 2582
SinI GGWCC 4 cut(s) 917, 1976, 2132, 2923
SlaI CTCGAG 2 cut(s) 110, 2582
SmiMI CAYNNNNRTG 6 cut(s) 225, 315, 596, 1854, 2082, 2739
SmlI CTYRAG 2 cut(s) 110, 2582
SmoI CTYRAG 2 cut(s) 110, 2582
SphI GCATGC 2 cut(s) 298, 1073
SsiI CCGC 8 cut(s) 59, 153, 253, 291, 572, 1087, 1379, 2816
SspI AATATT 2 cut(s) 1385, 2527
StyD4I CCNGG 6 cut(s) 160, 1029, 2448, 2459, 2882, 2925
StyI CCWWGG 4 cut(s) 211, 831, 2571, 2635
TaaI ACNGT 7 cut(s) 960, 1823, 1853, 1943, 2416, 2434, 2896
TaqI TCGA 7 cut(s) 111, 1204, 1948, 2294, 2583, 2774, 2790
TatI WGTACW 5 cut(s) 52, 701, 1181, 1676, 2411
TauI GCSGC 1 cut(s) 294
Tru1I TTAA 8 cut(s) 143, 584, 966, 1518, 1644, 1982, 2189, 2609
Tru9I TTAA 8 cut(s) 143, 584, 966, 1518, 1644, 1982, 2189, 2609
TscAI CASTG 2 cut(s) 777, 2026
TseFI GTSAC 5 cut(s) 206, 351, 772, 1428, 2434
TseI GCWGC 3 cut(s) 116, 1996, 2795
Tsp45I GTSAC 5 cut(s) 206, 351, 772, 1428, 2434
TspGWI ACGGA 3 cut(s) 712, 758, 869
TspRI CASTG 2 cut(s) 777, 2026
Tth111I GACNNNGTC 1 cut(s) 2432
Van91I CCANNNNNTGG 1 cut(s) 2889
VpaK11BI GGWCC 4 cut(s) 917, 1976, 2132, 2923
VspI ATTAAT 1 cut(s) 966
XagI CCTNNNNNAGG 1 cut(s) 1059
XapI RAATTY 9 cut(s) 22, 706, 893, 941, 1207, 1265, 1276, 1724, 2300
XbaI TCTAGA 3 cut(s) 47, 476, 1718
XceI RCATGY 5 cut(s) 59, 298, 560, 1073, 1409
XcmI CCANNNNNNNNNTGG 2 cut(s) 722, 2084
XhoI CTCGAG 2 cut(s) 110, 2582
XmaJI CCTAGG 1 cut(s) 2635
XmiI GTMKAC 2 cut(s) 445, 740
XmnI GAANNNNTTC 1 cut(s) 2300
ZrmI AGTACT 2 cut(s) 1183, 2413
Zsp2I ATGCAT 5 cut(s) 6, 339, 558, 973, 1920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.