MD02G1262400.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001328
Physical Location & Seq
Forward (+)
31672324 .. 31676648
4325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1262400.v1.1.491

Sequence Viewer

Length: 2382 bp
ATGGCGAACTCACATCTTCTTCTTCTTCTGTTGATATCCTCCCTCTTCTCCGTCCACCACTCAGGTGCTGCTACTGAGTCGGAGTCTGAGAGCCTTCTGAAAATCCAGCAGCTCCTCAACTACCCACCACCTCTAACCCTAGTCAGTTCCAAGGACGGACACTCTTTGTGCAGCGTTGAACCAACTCCTTCCCTCACTCTCTCTTGCTACCAAGGCAACGTTACCCAGCTTCACATTTCAGGCGGCGGAGCTGCCTTCCCTCCGCTGCCGCGTGACTTCTCAACCCACTCTCTCTTCTCCGCTCTCGCCTCCGCCTTCCCAACCCTCAAAGTCCTATCTCTGGTCTCTCTCCGCCTCTGGGGCCCACTCCCTCCCTCCCTCGCCCGTTTATCTTCCCTCGAGATTCTCAACCTCACCTCCAACTACCTCAGCGGCGCCATCCCTCCCCACATTTCCTCCCTCGTCAACCTCCAGACTCTCATCCTCGACCGCAACAACTTCACCGGCCGACTCCCGCCTTCGCTGGCTTCGCTTCCGTCGTTGGCCGTTCTCAGTTTGAACCACAACCTCCTCAACGGCTTTCTCCCGCGTTCTCTGGCATCTCTGCATACCCTCAGAGTCCTCTCTCTCTCCCACAACTTCCTGGCCGGCGAAGTTCCTGACCTCAAAAGCCTCACAAACTTGCAACTTCTTGATTTGGAAGCTAACTATTTCGGGCCTCGTTTTCCAAGCTTACGTTCCAAGAACAAGCTGGTGACCCTTGTGCTCCGAAACAACAAGTTTCGCCTTGGAATCCAGACCGGATTCCGAGGCGGTTTTCAGCTCCAGAGGCTTGACATTTCACTCAATCAATTCGTGGGGCCTTTTTCGCCTTCGTGGCTGTCGCTGCCTTCCATCAATTATCTTGACATTGCCGGAAATAAATTCACCGGAGTGCTCTCCAAGAACATGTCTTGCAATGCTGAGCTTGCTTTTGTGAATTTGTCCTCAAATCTTTTGGCAGGGGACTTGCCCACTTGCCTCAAAAACCGGGGTTCGAAGAGCAGCAGTGTGGTTGTTCTCTATTCCGGGAATTGTTTGACGAATGTGGACCAGAAGCAGCCGCAGCATCCTTCTAACTTGTGCCACAATGAAGCTCTGGCTGTGGAGATACCGCCTTCTGGTGACGAAAAGCGCAAGAGGCTTCATGATAATAAACAAGTTGCATCAAGAGCGGTGGCAGGCATTGTGGGTGCCGCTGCAATTGTTGCTCTGGCTTTCATGGCTGTTAAGTGGTTATACGGCAAACAGCTGCCTGTTAAAAGTCCCAGAACACGGTTGATTAGAGATTCAGCTTCAACAGTCAACACAGCCAAGCTACTCTCGGATGCAAAATATATATCAGAAACAATGAAGCTGGGAGCCAGCATTCCTGCTTATCGAACATTTTCTTTGGAGGAGCTCAAGGAAGCCACGAATGACTTTGATGATTCGACATTACTTGGTGAAGGCAAACATGGACAGATGTATAGAGGGATGCTCACCAACGGAACACTTGTTGCTATCAGAGGCTTGAAAATGAGAAAGAGGCAGGCTCCGCAAGTCTACACGCACCACCTTGAACTGATTTCAAAACTGAGACATAGCCATTTGGTTAGCGCTCTTGGACATTGCTTGGAGTACCATCCCGATGATTCAGGCATCAGCAGAATATTTCTTGTATTTGAGTTTGTTCCAAATGGAACTCTAAGAGGCTGCATTTCTGAAGGACCCCCAGAACGAAAGCTTACTTGGGCGCAGAGAATAGTAGCTGCAATTGGAGTTGCAAAGGGTATTCAGTTTCTGCATACAGGGATTGTTCCTGGTGTAAAATCAAACAATCTCAAGATAACAAATGTCTTATTGGATCACGATCTTCATGTGAAAATAAGCAGTTATAACCTACCTCTTTTAGCTGAAAACAACAGGGGAACGGTGGGTACTACAGTTTCTTCTCCTGCACCGAAAGGAAGCATTCAAACTAGGGTTAACCATGAGTGTAAGAACGACGTTTATGATATCGGAGTAATCTTGCTCGAAATCATTTTGGGGAGGCCTATCATGTTCCAAAATGAAGTTCGTGTTTTGAAAGACCTTTTACAAGTAAGTTTGACCACCGATGACACAGCTCGAAGGAGCATTGTGGATCCAGCGGTGCACAAGGAATGCTCGGATGAATCGCTGAAGACAATGATGGAGATATGTGTGAGATGTCTTTCCGATGAGGCAGCTGATAGGCCTTCAGTCGAAGACATTCTTTGGAATCTGCAGTTTGCAGCGCAGGTTCAGGATTCAGCGAGATATGACTTTTTGAGTAGTAATCAGGGCTCGCCCGTTTCATCACCCCAACTCCAGCTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

794

Amino Acids

86.57

Weight (kDa)

9.08

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 156 - 213 5.4e-08 Leucine rich repeat
Pkinase PF00069 490 - 759 3.7e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 493 - 760 1.1e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1917
Acc36I ACCTGC 1 cut(s) 2290
AccB1I GGYRCC 2 cut(s) 434, 1232
AccBSI CCGCTC 2 cut(s) 302, 1214
AccI GTMKAC 1 cut(s) 1584
AccII CGCG 2 cut(s) 271, 589
AclI AACGTT 1 cut(s) 219
AclWI GGATC 3 cut(s) 1893, 2159, 2172
AcoI YGGCCR 3 cut(s) 505, 543, 645
AcsI RAATTY 2 cut(s) 923, 979
AcuI CTGAAG 3 cut(s) 1764, 2222, 2244
AcyI GRCGYC 1 cut(s) 435
AfaI GTAC 2 cut(s) 1661, 1960
AfeI AGCGCT 1 cut(s) 1639
AfiI CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1314
AflIII ACRYGT 1 cut(s) 948
AgsI TTSAA 8 cut(s) 179, 559, 1338, 1555, 1601, 1611, 1997, 2107
AjnI CCWGG 2 cut(s) 642, 1840
AjuI GAANNNNNNNTTGG 6 cut(s) 721, 753, 1753, 1785, 2079, 2111
AleI CACNNNNGTG 2 cut(s) 63, 932
Alw21I GWGCWC 4 cut(s) 768, 939, 1443, 2178
Alw26I GTCTC 2 cut(s) 349, 1612
Alw44I GTGCAC 1 cut(s) 2174
AlwI GGATC 3 cut(s) 1893, 2159, 2172
AlwNI CAGNNNCTG 2 cut(s) 68, 1822
Ama87I CYCGRG 1 cut(s) 398
Aor51HI AGCGCT 1 cut(s) 1639
AoxI GGCC 8 cut(s) 361, 505, 543, 645, 716, 860, 2072, 2255
ApaI GGGCCC 1 cut(s) 365
ApaLI GTGCAC 1 cut(s) 2174
ApoI RAATTY 2 cut(s) 923, 979
Asp700I GAANNNNTTC 2 cut(s) 1426, 2271
AspLEI GCGC 5 cut(s) 437, 1176, 1640, 1777, 2299
AspS9I GGNCC 6 cut(s) 361, 362, 716, 860, 1090, 1748
AsuC2I CCSGG 2 cut(s) 1031, 1069
AsuHPI GGTGA 8 cut(s) 406, 493, 766, 919, 1175, 1496, 1513, 2352
AsuII TTCGAA 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 2 cut(s) 1090, 1748
BaeGI GKGCMC 2 cut(s) 365, 2178
BaeI ACNNNNGTAYC 2 cut(s) 1950, 1983
BamHI GGATCC 1 cut(s) 2164
BanI GGYRCC 2 cut(s) 434, 1232
BanII GRGCYC 3 cut(s) 365, 1443, 2348
BbsI GAAGAC 2 cut(s) 2210, 2274
Bbv12I GWGCWC 4 cut(s) 768, 939, 1443, 2178
BbvCI CCTCAGC 1 cut(s) 428
BccI CCATC 4 cut(s) 446, 902, 1671, 2206
BceAI ACGGC 3 cut(s) 530, 592, 1297
BciT130I CCWGG 2 cut(s) 644, 1842
BcnI CCSGG 2 cut(s) 1031, 1069
BcoDI GTCTC 2 cut(s) 349, 1612
BfaI CTAG 2 cut(s) 140, 2001
BfmI CTRYAG 2 cut(s) 1962, 2285
BfoI RGCGCY 2 cut(s) 438, 1641
BfuAI ACCTGC 1 cut(s) 2290
BglI GCCNNNNNGGC 2 cut(s) 360, 877
BlpI GCTNAGC 1 cut(s) 963
Bme1390I CCNGG 4 cut(s) 644, 1031, 1069, 1842
Bme18I GGWCC 2 cut(s) 1090, 1748
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 6 cut(s) 361, 362, 716, 860, 1090, 1748
BmiI GGNNCC 9 cut(s) 362, 363, 436, 861, 1234, 1402, 1575, 1750, 2166
BmrFI CCNGG 4 cut(s) 644, 1031, 1069, 1842
BmsI GCATC 6 cut(s) 608, 1117, 1214, 1357, 1506, 1689
BpiI GAAGAC 2 cut(s) 2210, 2274
BplI GAGNNNNNCTC 4 cut(s) 1503, 1535, 1558, 1590
BpmI CTGGAG 3 cut(s) 455, 809, 2354
Bpu10I CCTNAGC 1 cut(s) 428
Bpu1102I GCTNAGC 1 cut(s) 963
Bpu14I TTCGAA 1 cut(s) 1037
BpuEI CTTGAG 2 cut(s) 1427, 1847
BpuMI CCSGG 2 cut(s) 1031, 1069
BsaBI GATNNNNATC 1 cut(s) 1890
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 5 cut(s) 150, 211, 787, 808, 1030
BsaWI WCCGGW 2 cut(s) 800, 929
BsaXI ACNNNNNCTCC 6 cut(s) 401, 431, 440, 470, 2352, 2382
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1314
Bse118I RCCGGY 2 cut(s) 503, 647
Bse3DI GCAATG 3 cut(s) 909, 964, 1648
Bse8I GATNNNNATC 1 cut(s) 1890
BseBI CCWGG 2 cut(s) 644, 1842
BseDI CCNNGG 5 cut(s) 150, 211, 787, 808, 1030
BseGI GGATG 7 cut(s) 438, 480, 1108, 1372, 1521, 1663, 2197
BseJI GATNNNNATC 1 cut(s) 1890
BseLI CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1314
BseMI GCAATG 3 cut(s) 909, 964, 1648
BseMII CTCAG 8 cut(s) 66, 75, 78, 442, 565, 628, 954, 1607
BseRI GAGGAG 3 cut(s) 104, 560, 1451
BseSI GKGCMC 2 cut(s) 365, 2178
BseX3I CGGCCG 1 cut(s) 505
BseYI CCCAGC 2 cut(s) 225, 1396
BsgI GTGCAG 2 cut(s) 190, 1962
Bsh1236I CGCG 2 cut(s) 271, 589
Bsh1285I CGRYCG 2 cut(s) 490, 508
BshFI GGCC 8 cut(s) 363, 507, 545, 647, 718, 862, 2074, 2257
BshNI GGYRCC 2 cut(s) 434, 1232
BsiEI CGRYCG 2 cut(s) 490, 508
BsiHKAI GWGCWC 4 cut(s) 768, 939, 1443, 2178
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 7 cut(s) 504, 648, 801, 915, 930, 1030, 1068
BslFI GGGAC 2 cut(s) 1019, 1290
BslI CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1314
BsmAI GTCTC 2 cut(s) 349, 1612
BsmFI GGGAC 2 cut(s) 1019, 1290
BsmI GAATGC 3 cut(s) 1407, 1992, 2189
BsnI GGCC 8 cut(s) 363, 507, 545, 647, 718, 862, 2074, 2257
Bso31I GGTCTC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 398
Bsp119I TTCGAA 1 cut(s) 1037
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 6 cut(s) 365, 768, 939, 1443, 2178, 2348
Bsp143I GATC 3 cut(s) 1885, 1891, 2164
Bsp1720I GCTNAGC 1 cut(s) 963
BspANI GGCC 8 cut(s) 363, 507, 545, 647, 718, 862, 2074, 2257
BspCNI CTCAG 8 cut(s) 67, 74, 79, 441, 564, 627, 955, 1608
BspFNI CGCG 2 cut(s) 271, 589
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 9 cut(s) 362, 363, 436, 861, 1234, 1402, 1575, 1750, 2166
BspMAI CTGCAG 1 cut(s) 2289
BspMI ACCTGC 1 cut(s) 2290
BspPI GGATC 3 cut(s) 1893, 2159, 2172
BspQI GCTCTTC 1 cut(s) 1034
BspT104I TTCGAA 1 cut(s) 1037
BspT107I GGYRCC 2 cut(s) 434, 1232
BspTNI GGTCTC 1 cut(s) 349
BsrBI CCGCTC 2 cut(s) 302, 1214
BsrDI GCAATG 3 cut(s) 909, 964, 1648
BsrFI RCCGGY 2 cut(s) 503, 647
BssAI RCCGGY 2 cut(s) 503, 647
BssECI CCNNGG 5 cut(s) 150, 211, 787, 808, 1030
BssMI GATC 3 cut(s) 1885, 1891, 2164
BssNI GRCGYC 1 cut(s) 435
BssT1I CCWWGG 3 cut(s) 150, 211, 787
Bst2UI CCWGG 2 cut(s) 644, 1842
Bst4CI ACNGT 4 cut(s) 1317, 1342, 1954, 1966
Bst6I CTCTTC 3 cut(s) 50, 299, 1034
BstACI GRCGYC 1 cut(s) 435
BstAPI GCANNNNNTGC 1 cut(s) 1247
BstBI TTCGAA 1 cut(s) 1037
BstC8I GCNNGC 7 cut(s) 525, 649, 969, 1222, 1405, 1572, 2348
BstDEI CTNAG 9 cut(s) 61, 75, 87, 428, 551, 614, 963, 1616, 1727
BstEII GGTNACC 1 cut(s) 754
BstF5I GGATG 7 cut(s) 438, 480, 1108, 1372, 1521, 1663, 2197
BstFNI CGCG 2 cut(s) 271, 589
BstH2I RGCGCY 2 cut(s) 438, 1641
BstHHI GCGC 5 cut(s) 437, 1176, 1640, 1777, 2299
BstKTI GATC 3 cut(s) 1888, 1894, 2167
BstMAI GTCTC 2 cut(s) 349, 1612
BstMBI GATC 3 cut(s) 1885, 1891, 2164
BstMCI CGRYCG 2 cut(s) 490, 508
BstNI CCWGG 2 cut(s) 644, 1842
BstNSI RCATGY 1 cut(s) 952
BstPI GGTNACC 1 cut(s) 754
BstSCI CCNGG 4 cut(s) 642, 1029, 1067, 1840
BstSFI CTRYAG 2 cut(s) 1962, 2285
BstSLI GKGCMC 2 cut(s) 365, 2178
BstUI CGCG 2 cut(s) 271, 589
BstV2I GAAGAC 2 cut(s) 2210, 2274
BstX2I RGATCY 1 cut(s) 2164
BstYI RGATCY 1 cut(s) 2164
BstZI CGGCCG 1 cut(s) 505
BsuRI GGCC 8 cut(s) 363, 507, 545, 647, 718, 862, 2074, 2257
BtsCI GGATG 7 cut(s) 438, 480, 1108, 1372, 1521, 1663, 2197
BtsI GCAGTG 1 cut(s) 1054
BtsIMutI CAGTG 1 cut(s) 1054
BveI ACCTGC 1 cut(s) 2290
Cac8I GCNNGC 7 cut(s) 525, 649, 969, 1222, 1405, 1572, 2348
CaiI CAGNNNCTG 2 cut(s) 68, 1822
CciI TCATGA 1 cut(s) 1186
CfoI GCGC 5 cut(s) 437, 1176, 1640, 1777, 2299
Cfr10I RCCGGY 2 cut(s) 503, 647
Cfr13I GGNCC 6 cut(s) 361, 362, 716, 860, 1090, 1748
Csp6I GTAC 2 cut(s) 1660, 1959
CspCI CAANNNNNGTGG 2 cut(s) 1197, 1232
CviAII CATG 7 cut(s) 949, 1187, 1261, 1496, 1898, 2012, 2080
CviQI GTAC 2 cut(s) 1660, 1959
DdeI CTNAG 9 cut(s) 61, 75, 87, 428, 551, 614, 963, 1616, 1727
DinI GGCGCC 1 cut(s) 436
DpnI GATC 3 cut(s) 1887, 1893, 2166
DpnII GATC 3 cut(s) 1885, 1891, 2164
EaeI YGGCCR 3 cut(s) 505, 543, 645
EagI CGGCCG 1 cut(s) 505
Eam1104I CTCTTC 3 cut(s) 50, 299, 1034
EarI CTCTTC 3 cut(s) 50, 299, 1034
EciI GGCGGA 3 cut(s) 261, 301, 341
Ecl136II GAGCTC 1 cut(s) 1441
EclXI CGGCCG 1 cut(s) 505
Eco130I CCWWGG 3 cut(s) 150, 211, 787
Eco147I AGGCCT 2 cut(s) 2074, 2257
Eco24I GRGCYC 3 cut(s) 365, 1443, 2348
Eco31I GGTCTC 1 cut(s) 349
Eco32I GATATC 2 cut(s) 36, 2038
Eco47I GGWCC 2 cut(s) 1090, 1748
Eco47III AGCGCT 1 cut(s) 1639
Eco52I CGGCCG 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 1441
Eco57I CTGAAG 3 cut(s) 1764, 2222, 2244
Eco88I CYCGRG 1 cut(s) 398
Eco91I GGTNACC 1 cut(s) 754
EcoICRI GAGCTC 1 cut(s) 1441
EcoO109I RGGNCCY 3 cut(s) 361, 860, 1748
EcoO65I GGTNACC 1 cut(s) 754
EcoRII CCWGG 2 cut(s) 642, 1840
EcoRV GATATC 2 cut(s) 36, 2038
EcoT14I CCWWGG 3 cut(s) 150, 211, 787
EcoT38I GRGCYC 3 cut(s) 365, 1443, 2348
EgeI GGCGCC 1 cut(s) 436
EheI GGCGCC 1 cut(s) 436
ErhI CCWWGG 3 cut(s) 150, 211, 787
FaeI CATG 7 cut(s) 952, 1190, 1264, 1499, 1901, 2015, 2083
FalI AAGNNNNNCTT 2 cut(s) 2259, 2291
FaqI GGGAC 2 cut(s) 1019, 1290
FatI CATG 7 cut(s) 948, 1186, 1260, 1495, 1897, 2011, 2079
FauI CCCGC 2 cut(s) 522, 594
FblI GTMKAC 1 cut(s) 1584
FokI GGATG 7 cut(s) 425, 467, 1095, 1379, 1528, 1650, 2204
FriOI GRGCYC 3 cut(s) 365, 1443, 2348
FspBI CTAG 2 cut(s) 140, 2001
GlaI GCGC 5 cut(s) 436, 1175, 1639, 1776, 2298
GsaI CCCAGC 2 cut(s) 229, 1400
GsuI CTGGAG 3 cut(s) 455, 809, 2354
HaeII RGCGCY 2 cut(s) 438, 1641
HaeIII GGCC 8 cut(s) 363, 507, 545, 647, 718, 862, 2074, 2257
HapII CCGG 7 cut(s) 504, 648, 801, 915, 930, 1030, 1068
HhaI GCGC 5 cut(s) 437, 1176, 1640, 1777, 2299
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 7 cut(s) 952, 1190, 1264, 1499, 1901, 2015, 2083
Hin6I GCGC 5 cut(s) 435, 1174, 1638, 1775, 2297
HinP1I GCGC 5 cut(s) 435, 1174, 1638, 1775, 2297
HincII GTYRAC 3 cut(s) 466, 1345, 2008
HindII GTYRAC 3 cut(s) 466, 1345, 2008
HindIII AAGCTT 2 cut(s) 730, 1763
HpaI GTTAAC 1 cut(s) 2008
HpaII CCGG 7 cut(s) 504, 648, 801, 915, 930, 1030, 1068
HphI GGTGA 8 cut(s) 406, 493, 766, 919, 1175, 1496, 1513, 2352
Hpy166II GTNNAC 7 cut(s) 55, 466, 1090, 1345, 1585, 2008, 2176
Hpy8I GTNNAC 7 cut(s) 55, 466, 1090, 1345, 1585, 2008, 2176
Hpy99I CGWCG 2 cut(s) 541, 2030
HpyCH4III ACNGT 4 cut(s) 1317, 1342, 1954, 1966
HpyCH4IV ACGT 3 cut(s) 219, 736, 2028
HpyF3I CTNAG 9 cut(s) 61, 75, 87, 428, 551, 614, 963, 1616, 1727
HpySE526I ACGT 3 cut(s) 219, 736, 2028
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 7 cut(s) 952, 1190, 1264, 1499, 1901, 2015, 2083
HspAI GCGC 5 cut(s) 435, 1174, 1638, 1775, 2297
KasI GGCGCC 1 cut(s) 434
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
KspAI GTTAAC 1 cut(s) 2008
Kzo9I GATC 3 cut(s) 1885, 1891, 2164
LguI GCTCTTC 1 cut(s) 1034
LmnI GCTCC 8 cut(s) 117, 248, 771, 828, 1400, 1438, 1579, 2154
LweI GCATC 6 cut(s) 608, 1117, 1214, 1357, 1506, 1689
MaeI CTAG 2 cut(s) 140, 2001
MaeII ACGT 3 cut(s) 219, 736, 2028
MaeIII GTNAC 4 cut(s) 220, 272, 754, 1163
MalI GATC 3 cut(s) 1887, 1893, 2166
MbiI CCGCTC 2 cut(s) 302, 1214
MboI GATC 3 cut(s) 1885, 1891, 2164
MfeI CAATTG 2 cut(s) 1242, 1794
MflI RGATCY 1 cut(s) 2164
MhlI GDGCHC 6 cut(s) 365, 768, 939, 1443, 2178, 2348
MluCI AATT 7 cut(s) 851, 898, 923, 979, 1072, 1242, 1794
Mly113I GGCGCC 1 cut(s) 435
MlyI GAGTC 5 cut(s) 86, 92, 469, 504, 627
MmeI TCCRAC 2 cut(s) 60, 444
MroNI GCCGGC 1 cut(s) 647
MroXI GAANNNNTTC 2 cut(s) 1426, 2271
MseI TTAA 3 cut(s) 1269, 1299, 2007
MslI CAYNNNNRTG 3 cut(s) 63, 932, 1668
MspA1I CMGCKG 6 cut(s) 265, 432, 1238, 1291, 2171, 2249
MspI CCGG 7 cut(s) 504, 648, 801, 915, 930, 1030, 1068
MspR9I CCNGG 4 cut(s) 644, 1031, 1069, 1842
MunI CAATTG 2 cut(s) 1242, 1794
Mva1269I GAATGC 3 cut(s) 1407, 1992, 2189
MvaI CCWGG 2 cut(s) 644, 1842
MvnI CGCG 2 cut(s) 271, 589
NaeI GCCGGC 1 cut(s) 649
NarI GGCGCC 1 cut(s) 435
NciI CCSGG 2 cut(s) 1031, 1069
NdeII GATC 3 cut(s) 1885, 1891, 2164
NgoMIV GCCGGC 1 cut(s) 647
NlaIII CATG 7 cut(s) 952, 1190, 1264, 1499, 1901, 2015, 2083
NlaIV GGNNCC 9 cut(s) 362, 363, 436, 861, 1234, 1402, 1575, 1750, 2166
NmuCI GTSAC 3 cut(s) 272, 754, 1163
NspI RCATGY 1 cut(s) 952
NspV TTCGAA 1 cut(s) 1037
OliI CACNNNNGTG 2 cut(s) 63, 932
PaeR7I CTCGAG 1 cut(s) 398
PagI TCATGA 1 cut(s) 1186
PceI AGGCCT 2 cut(s) 2074, 2257
PciI ACATGT 1 cut(s) 948
PciSI GCTCTTC 1 cut(s) 1034
PcsI WCGNNNNNNNCGW 1 cut(s) 536
PctI GAATGC 3 cut(s) 1407, 1992, 2189
PdiI GCCGGC 1 cut(s) 649
PdmI GAANNNNTTC 2 cut(s) 1426, 2271
PfeI GAWTC 9 cut(s) 403, 792, 804, 1328, 1469, 1673, 2195, 2281, 2309
PfoI TCCNGGA 1 cut(s) 1067
PleI GAGTC 5 cut(s) 85, 91, 469, 504, 626
PluTI GGCGCC 1 cut(s) 438
PpsI GAGTC 5 cut(s) 85, 91, 469, 504, 626
PpuMI RGGWCCY 1 cut(s) 1748
PscI ACATGT 1 cut(s) 948
PsiI TTATAA 1 cut(s) 1917
Psp124BI GAGCTC 1 cut(s) 1443
Psp1406I AACGTT 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 1748
Psp6I CCWGG 2 cut(s) 642, 1840
PspEI GGTNACC 1 cut(s) 754
PspFI CCCAGC 2 cut(s) 225, 1396
PspGI CCWGG 2 cut(s) 642, 1840
PspN4I GGNNCC 9 cut(s) 362, 363, 436, 861, 1234, 1402, 1575, 1750, 2166
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 6 cut(s) 361, 362, 716, 860, 1090, 1748
PspPPI RGGWCCY 1 cut(s) 1748
PsrI GAACNNNNNNTAC 2 cut(s) 1942, 1974
PstI CTGCAG 1 cut(s) 2289
PstNI CAGNNNCTG 2 cut(s) 68, 1822
PsuI RGATCY 1 cut(s) 2164
PvuII CAGCTG 2 cut(s) 1291, 2249
RsaI GTAC 2 cut(s) 1661, 1960
RsaNI GTAC 2 cut(s) 1660, 1959
RseI CAYNNNNRTG 3 cut(s) 63, 932, 1668
SacI GAGCTC 1 cut(s) 1443
SapI GCTCTTC 1 cut(s) 1034
SaqAI TTAA 3 cut(s) 1269, 1299, 2007
Sau3AI GATC 3 cut(s) 1885, 1891, 2164
Sau96I GGNCC 6 cut(s) 361, 362, 716, 860, 1090, 1748
SchI GAGTC 5 cut(s) 86, 92, 469, 504, 627
ScrFI CCNGG 4 cut(s) 644, 1031, 1069, 1842
SduI GDGCHC 6 cut(s) 365, 768, 939, 1443, 2178, 2348
SfaNI GCATC 6 cut(s) 608, 1117, 1214, 1357, 1506, 1689
SfcI CTRYAG 2 cut(s) 1962, 2285
SfoI GGCGCC 1 cut(s) 436
Sfr274I CTCGAG 1 cut(s) 398
SfuI TTCGAA 1 cut(s) 1037
SinI GGWCC 2 cut(s) 1090, 1748
SlaI CTCGAG 1 cut(s) 398
SmiMI CAYNNNNRTG 3 cut(s) 63, 932, 1668
SmlI CTYRAG 3 cut(s) 398, 1442, 1862
SmoI CTYRAG 3 cut(s) 398, 1442, 1862
Sse9I AATT 7 cut(s) 851, 898, 923, 979, 1072, 1242, 1794
SseBI AGGCCT 2 cut(s) 2074, 2257
SspDI GGCGCC 1 cut(s) 434
SspI AATATT 1 cut(s) 1692
SspMI CTAG 2 cut(s) 140, 2001
SstI GAGCTC 1 cut(s) 1443
StuI AGGCCT 2 cut(s) 2074, 2257
StyD4I CCNGG 4 cut(s) 642, 1029, 1067, 1840
StyI CCWWGG 3 cut(s) 150, 211, 787
TaaI ACNGT 4 cut(s) 1317, 1342, 1954, 1966
TaiI ACGT 3 cut(s) 222, 739, 2031
TaqI TCGA 8 cut(s) 399, 486, 1037, 1420, 1472, 2055, 2149, 2265
TasI AATT 7 cut(s) 851, 898, 923, 979, 1072, 1242, 1794
TauI GCSGC 5 cut(s) 246, 271, 435, 1105, 1238
TfiI GAWTC 9 cut(s) 403, 792, 804, 1328, 1469, 1673, 2195, 2281, 2309
Tru1I TTAA 3 cut(s) 1269, 1299, 2007
Tru9I TTAA 3 cut(s) 1269, 1299, 2007
TscAI CASTG 1 cut(s) 1054
TseFI GTSAC 3 cut(s) 272, 754, 1163
Tsp45I GTSAC 3 cut(s) 272, 754, 1163
TspDTI ATGAA 8 cut(s) 1146, 1175, 1249, 1406, 1886, 2106, 2208, 2346
TspGWI ACGGA 4 cut(s) 40, 171, 525, 1542
TspRI CASTG 1 cut(s) 1054
VneI GTGCAC 1 cut(s) 2174
VpaK11BI GGWCC 2 cut(s) 1090, 1748
XapI RAATTY 2 cut(s) 923, 979
XceI RCATGY 1 cut(s) 952
XcmI CCANNNNNNNNNTGG 1 cut(s) 748
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 1584
XmnI GAANNNNTTC 2 cut(s) 1426, 2271
XspI CTAG 2 cut(s) 140, 2001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.